CN104820039A - 天麻中天麻素的含量的测定方法 - Google Patents
天麻中天麻素的含量的测定方法 Download PDFInfo
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- CN104820039A CN104820039A CN201510242233.7A CN201510242233A CN104820039A CN 104820039 A CN104820039 A CN 104820039A CN 201510242233 A CN201510242233 A CN 201510242233A CN 104820039 A CN104820039 A CN 104820039A
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- gastrodin
- acetonitrile
- rhizoma gastrodiae
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- PUQSUZTXKPLAPR-UJPOAAIJSA-N Gastrodin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1=CC=C(CO)C=C1 PUQSUZTXKPLAPR-UJPOAAIJSA-N 0.000 title claims abstract description 24
- 238000000034 method Methods 0.000 title abstract description 11
- 241000305491 Gastrodia elata Species 0.000 title abstract 7
- 238000000605 extraction Methods 0.000 claims abstract description 27
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 11
- 238000004128 high performance liquid chromatography Methods 0.000 claims abstract description 8
- 238000000899 pressurised-fluid extraction Methods 0.000 claims abstract description 6
- 239000000843 powder Substances 0.000 claims abstract description 5
- 239000002904 solvent Substances 0.000 claims abstract description 5
- 239000000203 mixture Substances 0.000 claims abstract description 4
- 238000010298 pulverizing process Methods 0.000 claims abstract description 3
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 claims description 39
- PUQSUZTXKPLAPR-KSSYENDESA-N 4-(beta-D-Glucopyranosyloxy) benzyl alcohol Natural products O([C@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1)c1ccc(CO)cc1 PUQSUZTXKPLAPR-KSSYENDESA-N 0.000 claims description 19
- 229930193974 gastrodin Natural products 0.000 claims description 19
- PUQSUZTXKPLAPR-NZEXEKPDSA-N helicidol Natural products O([C@H]1[C@H](O)[C@H](O)[C@@H](O)[C@@H](CO)O1)c1ccc(CO)cc1 PUQSUZTXKPLAPR-NZEXEKPDSA-N 0.000 claims description 19
- 238000003556 assay Methods 0.000 claims description 16
- 241000305492 Gastrodia Species 0.000 claims description 14
- 229910019142 PO4 Inorganic materials 0.000 claims description 14
- 239000007864 aqueous solution Substances 0.000 claims description 14
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims description 14
- 239000010452 phosphate Substances 0.000 claims description 14
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 9
- 239000007788 liquid Substances 0.000 claims description 8
- 239000006228 supernatant Substances 0.000 claims description 7
- 238000001514 detection method Methods 0.000 claims description 6
- 239000000243 solution Substances 0.000 claims description 6
- 238000001704 evaporation Methods 0.000 claims description 3
- 239000002245 particle Substances 0.000 claims description 3
- 239000013595 supernatant sample Substances 0.000 claims description 3
- 238000010926 purge Methods 0.000 claims description 2
- 230000003068 static effect Effects 0.000 claims description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 5
- 238000001035 drying Methods 0.000 abstract description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 abstract 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 abstract 2
- 229910000147 aluminium phosphate Inorganic materials 0.000 abstract 1
- 238000007865 diluting Methods 0.000 abstract 1
- 238000004821 distillation Methods 0.000 abstract 1
- 238000005259 measurement Methods 0.000 abstract 1
- 238000002156 mixing Methods 0.000 abstract 1
- 239000000523 sample Substances 0.000 description 8
- 238000012360 testing method Methods 0.000 description 8
- 239000000706 filtrate Substances 0.000 description 4
- 238000000926 separation method Methods 0.000 description 4
- QXDMQSPYEZFLGF-UHFFFAOYSA-L calcium oxalate Chemical compound [Ca+2].[O-]C(=O)C([O-])=O QXDMQSPYEZFLGF-UHFFFAOYSA-L 0.000 description 3
- 239000013078 crystal Substances 0.000 description 3
- 239000012467 final product Substances 0.000 description 3
- 238000010790 dilution Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 239000011259 mixed solution Substances 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 238000010992 reflux Methods 0.000 description 2
- 239000002023 wood Substances 0.000 description 2
- AFVOYXASQATZMK-UHFFFAOYSA-N 2,3-diacetyloxypropyl acetate hydrate Chemical compound O.CC(=O)OCC(COC(C)=O)OC(C)=O AFVOYXASQATZMK-UHFFFAOYSA-N 0.000 description 1
- ZCYVEMRRCGMTRW-UHFFFAOYSA-N 7553-56-2 Chemical compound [I] ZCYVEMRRCGMTRW-UHFFFAOYSA-N 0.000 description 1
- 229920000832 Cutin Polymers 0.000 description 1
- 241000233855 Orchidaceae Species 0.000 description 1
- 241000293001 Oxytropis besseyi Species 0.000 description 1
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- 241000238370 Sepia Species 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
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- 235000009508 confectionery Nutrition 0.000 description 1
- 239000007799 cork Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 210000002615 epidermis Anatomy 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 229910052740 iodine Inorganic materials 0.000 description 1
- 239000011630 iodine Substances 0.000 description 1
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- 229920001282 polysaccharide Polymers 0.000 description 1
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- 239000013558 reference substance Substances 0.000 description 1
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- Investigating Or Analysing Biological Materials (AREA)
Abstract
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Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105954379A (zh) * | 2016-04-22 | 2016-09-21 | 广西壮族自治区梧州食品药品检验所 | 一种通宣理肺丸中盐酸麻黄碱的萃取方法 |
CN105974006A (zh) * | 2016-04-26 | 2016-09-28 | 广西壮族自治区梧州食品药品检验所 | Ase-hplc测定桔梗中桔梗皂苷的方法 |
CN106770755A (zh) * | 2016-12-15 | 2017-05-31 | 中国中医科学院中药研究所 | 一种鉴定天麻产品的原料为新鲜天麻或干燥天麻的方法 |
CN106732473A (zh) * | 2016-11-14 | 2017-05-31 | 广西民族大学 | 一种酯化修饰的松香基高分子色谱柱分离天麻素的方法 |
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CN1515904A (zh) * | 2003-01-10 | 2004-07-28 | 贵州宏宇药业有限公司 | 一种天麻素含量测定方法 |
CN101149361A (zh) * | 2007-09-06 | 2008-03-26 | 长沙理工大学 | 一种天麻药材的鉴别方法 |
CN101732589A (zh) * | 2010-02-09 | 2010-06-16 | 长沙理工大学 | 天麻生化指纹图谱分类与品质鉴定技术及其应用 |
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CN101972414A (zh) * | 2010-11-05 | 2011-02-16 | 贵州益康制药有限公司 | 一种全天麻胶囊指纹图谱的建立方法及其指纹图谱 |
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CN104569166A (zh) * | 2013-10-16 | 2015-04-29 | 西安千禾药业有限责任公司 | 一种治疗癫痫抽搐、小儿惊风、面肌痉挛的药物组合物痫愈的检测方法 |
CN104306745A (zh) * | 2014-10-31 | 2015-01-28 | 云南永孜堂制药有限公司 | 一种天麻醒脑胶囊的质量控制方法 |
CN104483435A (zh) * | 2014-12-03 | 2015-04-01 | 贵阳德昌祥药业有限公司 | 一种天麻灵芝颗粒的检测方法 |
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Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105954379A (zh) * | 2016-04-22 | 2016-09-21 | 广西壮族自治区梧州食品药品检验所 | 一种通宣理肺丸中盐酸麻黄碱的萃取方法 |
CN105974006A (zh) * | 2016-04-26 | 2016-09-28 | 广西壮族自治区梧州食品药品检验所 | Ase-hplc测定桔梗中桔梗皂苷的方法 |
CN106732473A (zh) * | 2016-11-14 | 2017-05-31 | 广西民族大学 | 一种酯化修饰的松香基高分子色谱柱分离天麻素的方法 |
CN106732473B (zh) * | 2016-11-14 | 2019-10-08 | 广西民族大学 | 一种酯化修饰的松香基高分子色谱柱分离天麻素的方法 |
CN106770755A (zh) * | 2016-12-15 | 2017-05-31 | 中国中医科学院中药研究所 | 一种鉴定天麻产品的原料为新鲜天麻或干燥天麻的方法 |
CN106770755B (zh) * | 2016-12-15 | 2019-02-15 | 中国中医科学院中药研究所 | 一种鉴定天麻产品的原料为新鲜天麻或干燥天麻的方法 |
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