CN104813831B - A kind of indoor fast breeding method of wild barley - Google Patents
A kind of indoor fast breeding method of wild barley Download PDFInfo
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- CN104813831B CN104813831B CN201510211035.4A CN201510211035A CN104813831B CN 104813831 B CN104813831 B CN 104813831B CN 201510211035 A CN201510211035 A CN 201510211035A CN 104813831 B CN104813831 B CN 104813831B
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Abstract
The invention discloses a kind of indoor fast breeding method of wild barley, be sprouting pre-treatment will to be carried out with gibberellin after wild barley seed K cryogenic treatment, sprouted after flushing, the use of nutrient solution is the nutrient solution containing NaCl, seedling will be sprouted carries out illumination cultivation, and culture medium is water planting；Growth of seedling is sprouted after 14～20 days, the seedling for selecting neat and consistent is transplanted to earth culture environment；Poured with the double nutrient solution containing NaCl, illumination cultivation.Beneficial effects of the present invention are：Solve Wild Grass wild barley to sprout indoors slowly, plant is small and weak, slow problem of growing, and can quickly abolish the seed dormancy of wild barley and make its germination rate in 3～5 days reach more than 80%, and it is neat to emerge, seedling grows vigorous in nutrient solution containing NaCl.The method desk research wild barley biological characteristics are sought peace salt-tolerant drought-resistant is eliminating the aspects such as weak, the biology poor repeatability that causes slowly of development of plant strain growth with important value.
Description
Technical field
The present invention relates to plant planting field, and in particular to a kind of indoor fast breeding method of wild barley.
Background technology
Wild barley (Hordeum brevisubulatum (Trin.) Link) belongs to the perennial grass of grass family Hordeum
This plant, as one of most strong halophytes of salt tolerance in grass family Tribe Triticeae, has close relationship to close with barley, wheat
System.The features such as which has yield height, good palatability, is of high nutritive value, by as crops and the advantage of herbage hybrid new breed
Germ plasm resource.Hybrid experiment is carried out with herbages such as lyme grass at present, cultivated production performance height, the strong military supplies of salt-resistance
No. 1 wild barley.But as which belongs to wild germplasm, when being cultivated indoors, maximum challenge is exactly that seed germination rate is low, raw
The problems such as growing slow, plant is small and weak；If not making specially treated, its germination rate is only capable of reaching 30～50% under household condition, length
To 4 leaf phases need 40～50 days, and blade tiny, jaundice, be unfavorable for carrying out scientific research as research material.
Research shows that, used as the wild barley of halophytes, appropriate NaCl can promote the sprouting of wild barley seed, life
Long and photosynthesis, but a kind of based on NaCl currently without developing, it is suitable for quickly sprouting in wild barley room
Send out, strengthen plant strain growth, improve the effective ways of young plant regularity.
Content of the invention
The purpose of the present invention is aiming at above-mentioned defect of the prior art, there is provided a kind of interior of wild barley is quick to train
Educate method.
To achieve these goals, the technical scheme of present invention offer is：A kind of indoor fast breeding method of wild barley,
Comprise the following steps：
1) by wild barley seed impurity elimination, the seed of neat and consistent is selected in -20 DEG C～-10 DEG C 24～48h of K cryogenic treatment, it
Seed is carried out using the gibberellin of 80～200mg/L afterwards and pre-treatment is sprouted, obtain pre-treatment wild barley seed；
2) by step 1) the pre-treatment wild barley seed that obtains, with the kind for being uniformly sprinkling upon seed after distilled water flushing and sprouting box
Sprouted in subrack, the nutrient solution that sprouts in box is the nutrient solution containing 5～20mmol/L NaCl；The indoor conditions of sprouting
For 20 DEG C～26 DEG C, 16h and 16 DEG C～18 DEG C, 8h alternately, dark surrounds, 3～5 days afterwards wild barley seed can sprout
Send out, obtain sprouting seedling；
3) by step 2) the sprouting seedling that obtains, illumination cultivation is carried out, intensity of illumination is 600 μm of ol/ (m2S), cultivate
Medium is water planting, and mill water culture nutrient solution composition is the nutrient solution containing 25～75mmol/L NaCl, and illumination cultivation method is 24 DEG C～26
DEG C, 16h illumination and 15 DEG C～18 DEG C, 8h no light, alternately；
4) after step 3) in sprouting growth of seedling after 14～20 days, plant height has 3～5 leaves to 5cm, selects neat
Consistent seedling is transplanted to earth culture environment；Earth culture environment is one using the organic soil of air permeable humidity retaining, per 3～5 young plants
Group, per group keep 10cm × 10cm distance, soil moisture 70～85%, indoor humidity 30～50%, remaining growth conditions with
Step 3) identical；
5) by step 4) in the seedling transplanted to earth culture environment entered with the double nutrient solution containing 100～300mmol/L NaCl
Row is poured, and intensity of illumination is adjusted to 1000～2000 μm of ol/ (m2S), remaining growth conditions and step 4) identical.
Further, a kind of indoor fast breeding method of above-mentioned wild barley, the step 1) in seed sprout before
Processing procedure is by after wild barley seed distilled water flushing, is carried out at immersion with Gibberellins solution under 0～4 DEG C of cryogenic conditions
Reason.
Further, the indoor fast breeding method of above-mentioned a kind of wild barley, all nutrient compositions are consisted of：
KNO35.0～7.5mmol/L, NH4H2PO41.5～2.0mmol/L, Ca (NO3)20.4～0.6mmol/L, MgSO40.3～
0.6mmol/L, Fe-Citrate 40～70 μm of ol/L, H3BO380～100 μm of ol/L, MnCl2·4H215～20 μm of ol/ of O
L, ZnSO4·7H2O 1.4～1.8 μm of ol/L, CuSO4·5H2O 0.4～0.7 μm of ol/L, (NH4)6Mo7O24·4H2O 0.5～
0.9μmol/L.
Beneficial effects of the present invention are：A kind of indoor fast breeding method of wild barley that the present invention is provided, solves open country
Raw herbage wild barley is sprouted slowly indoors, and plant is small and weak, and slow problem of growing, the seed that can quickly abolish wild barley are stopped
Sleep and make its germination rate in 3～5 days that more than 80% is reached, and it is neat to emerge, seedling grows vigorous in nutrient solution containing NaCl.
The method is sought peace to desk research wild barley biological characteristics, and plant strain growth is weak, the biology that causes slowly of development eliminating for salt-tolerant drought-resistant
Learn the aspects such as poor repeatability and there is important value.
Description of the drawings
Fig. 1 is processed to Na in wild barley tissue for different NaCl concentration+Concentration affects.
Fig. 2 is processed for different NaCl concentration to be affected on wild barley Net Photosynthetic Rate.
Fig. 3 is the impact of embodiment 1-3 and normal control to wild barley percentage of seedgermination.
Fig. 4 is the impact of plant height after embodiment 1-3 and normal control are grown 30 days to wild barley.
Specific embodiment
Embodiment 1：
A kind of indoor fast breeding method of wild barley, comprises the steps：
1) by wild barley seed impurity elimination, the seed of neat and consistent is selected in -20 DEG C of K cryogenic treatment 48h, wild barley seed is used
Gibberellin after distilled water flushing using 100mg/L carries out the front process of seed sprouting under 0～4 DEG C of cryogenic conditions；
2) by step 1) wild barley seed distilled water is simply rinsed after the pre-treatment that obtains, and uniformly it is sprinkling upon seed afterwards and sprouts
Sprouted in the kind subrack for sending out box, sprouted the nutrient solution that the nutrient solution in box is containing 5mmol/L NaCl；The indoor bar of sprouting
Part is 24/18 DEG C, dark, 3～5 days afterwards wild barley seed can sprout, germination rate can reach 82%；
3) by step 2) sprout the seedling that obtains illumination cultivation is changed to, 25 DEG C, 16h illumination and 16 DEG C, 8h no light, alternately
Carry out, intensity of illumination is 600 μm of ol/ (m2S), culture medium is water planting, and composition is that the nutrition containing 25mmol/L NaCl is molten
Liquid；
4) after step 3) in growth of seedling after 20 days, plant height has 3～5 leaves, about grows to 5cm or so, you can select
The seedling of neat and consistent is transplanted to earth culture environment, should keep earth culture environment using the organic soil of air permeable humidity retaining during transplanting
Earth, every 5 young plant are one group, keep the distance of 10 × 10cm, soil moisture 70%, indoor humidity 40%, remaining growth bar per group
The same step 3) of part；
5) step 4) in transplant after seedling poured with the double nutrient solution containing 100mmol/L NaCl, change illumination strong
Spend for 1500 μm of ol/ (m2S), the same step 4) of remaining growth conditions；Wild barley grows vigorous, high uniformity with this understanding,
Leaf color is strong, can guarantee that the smooth development of correlative study；
Nutrient composition in the present embodiment is set as：7.5mmol/L KNO3, 2.0mmol/L NH4H2PO4,
0.6mmol/L Ca(NO3)2, 0.6mmol/L MgSO4, 70 μm of ol/L Fe-Citrate, 100 μm of ol/L H3BO3, 20 μm of ol/L
MnCl2·4H2O, 1.8 μm of ol/L ZnSO4·7H2O, 0.7 μm of ol/L CuSO4·5H2O, 0.9 μm of ol/L (NH4)6Mo7O24·
4H2O.
Embodiment 2：
Fast breeding method in a kind of wild barley room, identical with 1 step of embodiment, simply have adjusted associated temperature and NaCl
Concentration, mainly comprises the steps：
1) by wild barley seed impurity elimination, the seed of neat and consistent is selected in -18 DEG C of K cryogenic treatment 48h, wild barley seed is used
Carry out the front process of seed sprouting after after distilled water flushing using the gibberellin of 150mg/L under 0～4 DEG C of cryogenic conditions；
2) by step 1) wild barley seed distilled water is simply rinsed after the pre-treatment that obtains, and uniformly it is sprinkling upon seed afterwards and sprouts
Sprouted in the kind subrack for sending out box, sprouted the nutrient solution that the nutrient solution in box is containing 10mmol/L NaCl；The indoor bar of sprouting
Part is 26 DEG C of 16h/16 DEG C of 8h, dark, 3～5 days afterwards wild barley seed can sprout, germination rate can reach 85%；
3) by step 2) sprout the seedling that obtains illumination cultivation is changed to, 24 DEG C, 16h illumination and 15 DEG C, 8h no light, alternately
Carry out, intensity of illumination is 600 μm of ol/ (m2S), culture medium is water planting, and composition is that the nutrition containing 50mmol/L NaCl is molten
Liquid；
4) after step 3) in growth of seedling after 14～20 days, plant height has 3～5 leaves, about grows to 5cm or so, you can
The seedling for selecting neat and consistent is transplanted to earth culture environment；Earth culture environment should be kept during transplanting using the organic matter of air permeable humidity retaining
Soil, is one group per 3～5 young plants, keeps the distance of 10 × 10cm, soil moisture 75%, indoor humidity 45%, its remaining years per group
The same step 3) of elongate member；
5) step 4) in transplant after seedling poured with the double nutrient solution containing 150mmol/L NaCl, change illumination strong
Spend for 2000 μm of ol/ (m2S), the same step 4) of remaining growth conditions；Wild barley grows vigorous, high uniformity with this understanding,
Leaf color is strong, can guarantee that the smooth development of correlative study.
Nutrient composition in the present embodiment is set as：6.0mmol/L KNO3, 1.8mmol/L NH4H2PO4,
0.5mmol/L Ca(NO3)2, 0.5mmol/L MgSO4, 50 μm of ol/L Fe-Citrate, 90 μm of ol/L H3BO3, 20 μm of ol/L
MnCl2·4H2O, 1.5 μm of ol/L ZnSO4·7H2O, 0.5 μm of ol/L CuSO4·5H2O, 0.5 μm of ol/L (NH4)6Mo7O24·
4H2O.
Embodiment 3：
Fast breeding method in a kind of wild barley room, identical with 2 step of embodiment, simply have adjusted associated temperature, time
And NaCl concentration, mainly comprise the steps：
1) by wild barley seed impurity elimination, the seed of neat and consistent is selected in -16 DEG C of K cryogenic treatment 24h, wild barley seed is used
Carry out the front process of seed sprouting after after distilled water flushing using the gibberellin of 200mg/L under 0～4 DEG C of cryogenic conditions；
2) by step 1) wild barley seed distilled water is simply rinsed after the pre-treatment that obtains, and uniformly it is sprinkling upon seed afterwards and sprouts
Sprouted in the kind subrack for sending out box, sprouted the nutrient solution that the nutrient solution in box is containing 15mmol/L NaCl；The indoor bar of sprouting
Part is 20 DEG C of 16h/16 DEG C of 8h, dark, 3～5 days afterwards wild barley seed can sprout, germination rate can reach 80%；
3) by step 2) sprout the seedling that obtains illumination cultivation is changed to, 24 DEG C, 16h illumination and 15 DEG C, 8h no light, alternately
Carry out, intensity of illumination is 600 μm of ol/ (m2S), culture medium is water planting, and composition is that the nutrition containing 75mmol/L NaCl is molten
Liquid；
4) after step 3) in growth of seedling after 14～20 days, plant height has 3～5 leaves, about grows to 5cm or so, you can
The seedling for selecting neat and consistent is transplanted to earth culture environment.Earth culture environment should be kept during transplanting using the organic matter of air permeable humidity retaining
Soil, is one group per 3～5 young plants, keeps the distance of 10 × 10cm, soil moisture 70%, indoor humidity 50%, its remaining years per group
The same step 3) of elongate member；
5) step 4) in transplant after seedling poured with the double nutrient solution containing 300mmol/L NaCl, change illumination strong
Spend for 2000 μm of ol/ (m2S), the same step 4) of remaining growth conditions.Wild barley grows vigorous, high uniformity with this understanding,
Leaf color is strong, can guarantee that the smooth development of correlative study.
Nutrient composition in the present embodiment is set as：6.5mmol/L KNO3, 1.8mmol/L NH4H2PO4,
0.6mmol/L Ca(NO3)2, 0.5mmol/L MgSO4, 60 μm of ol/L Fe-Citrate, 90 μm of ol/L H3BO3, 15 μm of ol/L
MnCl2·4H2O, 1.8 μm of ol/L ZnSO4·7H2O, 0.7 μm of ol/L CuSO4·5H2O, 0.9 μm of ol/L (NH4)6Mo7O24·
4H2O.
The normal control refers to not do seed K cryogenic treatment and HORMONE TREATMENT, and condition of culture is also the condition on basis,
The wild barley cultural method of NaCl process is not done, specific as follows：
1) by wild barley seed impurity elimination, seed room temperature treatment 48h of neat and consistent is selected, wild barley seed is rushed with distilled water
The pre-treatment of seed sprouting is carried out after after washing using distilled water at ambient temperature；
2) by step 1) wild barley seed distilled water is simply rinsed after the pre-treatment that obtains, and uniformly it is sprinkling upon seed afterwards and sprouts
Sprouted in the kind subrack for sending out box, sprouted the nutrient solution that the nutrient solution in box is without NaCl；The indoor conditions of sprouting is room
Temperature, dark, wild barley seed can sprouting afterwards within 3～5 days；
3) by step 2) to sprout the seedling that obtains illumination cultivation is changed to, intensity of illumination is 600 μm of ol/ (m2S), the photoperiod
16/8h, room temperature, culture medium are water planting, and composition is the nutritive solution without NaCl；
4) after step 3) in growth of seedling after 20 days, plant height has 3～5 leaves, about grows to 5cm or so, you can select
The seedling of neat and consistent is transplanted to earth culture environment, should keep earth culture environment using the organic soil of air permeable humidity retaining during transplanting
Earth, every 5 young plant are one group, keep the distance of 10 × 10cm, soil moisture 70%, indoor humidity 40%, remaining growth bar per group
The same step 3) of part；
5) step 4) in transplant after seedling poured with the double nutrient solution without NaCl, intensity of illumination be 600 μ
mol/(m2S), the same step 4) of remaining growth conditions；Wild barley grows vigorous with this understanding, and high uniformity, leaf color are strong,
Can guarantee that the smooth development of correlative study；
Wherein, nutrient composition is set as：7.5mmol/L KNO3, 2.0mmol/L NH4H2PO4, 0.6mmol/L Ca
(NO3)2, 0.6mmol/L MgSO4, 70 μm of ol/L Fe-Citrate, 100 μm of ol/L H3BO3, 20 μm of ol/L MnCl2·
4H2O, 1.8 μm of ol/L ZnSO4·7H2O, 0.7 μm of ol/L CuSO4·5H2O, 0.9 μm of ol/L (NH4)6Mo7O24·4H2O.
Finally it should be noted that：The preferred embodiments of the present invention are the foregoing is only, the present invention are not limited to,
Although being described in detail to the present invention with reference to the foregoing embodiments, for a person skilled in the art, which still may be used
To modify to the technical scheme described in foregoing embodiments, or equivalent is carried out to which part technical characteristic.
All any modification, equivalent substitution and improvement that within the spirit and principles in the present invention, is made etc., should be included in the present invention's
Within protection domain.
Claims (2)
1. the indoor fast breeding method of a kind of wild barley, it is characterised in that comprise the following steps：
1）By wild barley seed impurity elimination, the seed of neat and consistent is selected in -20 DEG C～-10 DEG C 24～48h of K cryogenic treatment, make afterwards
Seed is carried out with the gibberellin of 80～200mg/L and sprouts pre-treatment, obtain pre-treatment wild barley seed；
2）By step 1）The pre-treatment wild barley seed for obtaining, with the kind subrack for being uniformly sprinkling upon seed sprouting box after distilled water flushing
On sprouted, seed sprout box in nutrient solution be the nutrient solution containing 5～20mmol/L NaCl；The indoor conditions of sprouting
For 20 DEG C～26 DEG C, 16h and 16 DEG C～18 DEG C, 8h alternately, dark surrounds, 3～5 days afterwards wild barley seed can sprout
Send out, obtain sprouting seedling；
3）By step 2）The sprouting seedling for obtaining, carries out illumination cultivation, and intensity of illumination is 600 μm of ol/ (m2S), culture medium is
Water planting, mill water culture nutrient solution composition are the nutrient solution containing 25～75mmol/L NaCl, and illumination cultivation method is 24 DEG C～26 DEG C, 16h
Illumination and 15 DEG C～18 DEG C, 8h no light, alternately；
4）Treat step 3）In sprouting growth of seedling after 14～20 days, plant height has 3～5 leaves, selects neat and consistent to 5cm
Seedling transplanted to earth culture environment；Earth culture environment is one group using the organic soil of air permeable humidity retaining, per 3～5 young plants, per
Group keeps the distance of 10cm × 10cm, soil moisture 70～85%, indoor humidity 30～50%, remaining growth conditions and step 3）Phase
With；
5）By step 4）Middle transplanting is poured to the seedling of earth culture environment with the nutrient solution containing 100～300 mmol/L NaCl,
Intensity of illumination is adjusted to 1000～2000 μm of ol/（m2S）, remaining growth conditions and step 4）Identical.
2. a kind of indoor fast breeding method of wild barley according to claim 1, it is characterised in that the step 1）In
Seed sprout pretreatment process be by after wild barley seed distilled water flushing, molten with gibberellin under 0～4 DEG C of cryogenic conditions
Liquid carries out immersion treatment.
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| CN106922515A (en) * | 2017-05-09 | 2017-07-07 | 中国农业科学院兰州畜牧与兽药研究所 | A kind of indoor fast breeding method of waste vetch |
| CN108849454A (en) * | 2018-08-03 | 2018-11-23 | 浙江大学 | A method of wheat seeds are quickly bred using the photoperiod |
| CN109197556B (en) * | 2018-09-20 | 2020-08-18 | 塔里木大学 | Barley seedling hydroponic device |
| CN111357588A (en) * | 2020-04-22 | 2020-07-03 | 福建农林大学 | Barley weed prevention seeding method |
| CN113149746A (en) * | 2021-05-19 | 2021-07-23 | 安徽金晟达生物电子科技有限公司 | Nutrient solution for continuous water culture pasture cultivation and preparation method thereof |
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| CN1502226A (en) * | 2002-11-20 | 2004-06-09 | 中国人民解放军军需大学 | Method for breeding salt-resisting short-arn barley |
| US20090246349A1 (en) * | 2005-07-11 | 2009-10-01 | Joanna Louise Mimica | Wheat pigment |
| PT103346B (en) * | 2005-09-13 | 2007-11-05 | Univ Evora | NET MEANS FOR THE PRODUCTION OF CHLAMYDOSPORIA POCHONIA CLAMIDOSPORTS |
| CN101385438A (en) * | 2008-10-29 | 2009-03-18 | 中国科学院东北地理与农业生态研究所 | Screening crop anti reversibility strain |
| CN102106259B (en) * | 2009-12-24 | 2013-07-17 | 上海市农业科学院 | Method for improving salt endurance of cereal crops |
| CN102860159A (en) * | 2012-10-10 | 2013-01-09 | 江苏省农业科学院 | Salt-tolerance determining method for barley at seedling stage |
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