CN104777245A - Cosmetic oxidative hair dye detection method - Google Patents

Abstract

The invention discloses a cosmetic oxidative hair dye detection method, which aims at five target objectives: p-phenylenediamine HCl, P-aminophenol, toluene-2,5-diamine sulfate, resorcinol, and m-aminophenol. The detection method comprises the following steps: step A, pre-treating a sample; step B, preparing a standard stock solution; step C, preparing a standard work solution; and step D, setting the analysis conditions. The dye components has the characteristics of strong polarity, low volatility, and instability, and the provided detection method can effectively control the uncertain factors and is capable of successfully and precisely finishing the detection.

Description

Oxidative hair dyes detection method in a kind of cosmetics

Technical field

The invention belongs in cosmetics safety detection field, be specifically related to oxidative hair dyes detection method in a kind of cosmetics.

Background technology

Along with improving constantly of living standard, people have started to focus on quality of life and consumption quality.Hair dyeing, as one of beauty treatment mode, has become a kind of pandemic fashion.Hair dye can be divided into oxidized form (persistence), Direct-type (temporary or semi-persistent), metallic salt and natural plant type etc.The hair dye of current use, based on oxidative hair dyes, has that Color is good, tonal variation is wide, a plurality of advantages such as long of holding time.The dye component that oxidative hair dyes uses belongs to aromatic amine compounds more, and this is the compound that a class has carcinogenicity, and thus its safety problem is extensively concerned.The allergic reaction caused by hair dye is very general, and Long-Time Service also may teratogenesis, mutagenesis, carcinogenic and cause endocrine effect etc.The various safety problem existed in view of hair dye and the contactee of huge number and user, various countries pay much attention to the administration of health of hair dye and day-to-day supervision in area.

Summary of the invention

(1) technical matters that will solve

In order to solve the problem, the present invention proposes oxidative hair dyes detection method in a kind of cosmetics is have strong polarity, low volatility and instable feature for dye component, the more rational method of testing of one of proposition.Can effectively control these uncertainties, make test smoothly and complete accurately.

(2) technical scheme

Oxidative hair dyes detection method in a kind of cosmetics, it is characterized in that for p-PDA HCl, P-amino-phenol, Toluene-2,4-diisocyanate, the detection of 5-diamines sulfate, resorcinol, these 5 kinds of objects of m-amino-phenol, this detection method needs 5 increment product; Its concrete steps are as follows:

A. sample pre-treatments: directly take sample 0.5g, adds several beaded glasses successively, 1% sodium sulfite solution 1mL, ethanol 12mL, 1% triethanolamine phosphate buffer (pH=7.7) 12mL; Concussion, makes sample disperse completely in the solution; Ultrasonic extraction 15min; With sample introduction analysis after 0.45 μm of organic membrane filtration;

B. standard reserving solution preparation: these four kinds of standard items ethanol of p-PDA HCl, P-amino-phenol, resorcinol, m-amino-phenol configure ,-18 DEG C of preservations; Toluene-2,4-diisocyanate, 5-diamines sulfate water-ethanol configures sample solution, regulates pH to be alkalescence, add sodium sulphite ,-18 DEG C of preservations with ammoniacal liquor;

C. standard working solution preparation: by standard reserving solution with 1% triethanolamine phosphate buffer (pH=7.7) be diluted to concentration be 0.5,1.0,2.5,5.0, the solution of 10.0ppm, now with the current;

D. analysis condition: SB C18 post (4.6 × 250mm, 5 μm), mobile phase: 1% triethanolamine phosphate buffer (pH=7.7)-acetonitrile=95: 5,1.5mL/min, room temperature is separated, and sample size 10 μ L, DAD detect, p-phenylenediamine and p-aminophenol determined wavelength is 305nm, and all the other are 280nm.

(3) beneficial effect

Oxidative hair dyes detection method in a kind of cosmetics that the present invention proposes, compared with prior art, it has following beneficial effect: the present invention be directed to dye component and have strong polarity, low volatility and instable feature, the more rational method of testing of one of proposition.Can effectively control these uncertainties, make test smoothly and complete accurately.

Accompanying drawing explanation

Fig. 1 is overall flow schematic diagram of the present invention.

Embodiment

As shown in Figure 1, oxidative hair dyes detection method in a kind of cosmetics, it is characterized in that for p-PDA HCl, P-amino-phenol, Toluene-2,4-diisocyanate, the detection of 5-diamines sulfate, resorcinol, these 5 kinds of objects of m-amino-phenol, this detection method needs 5 increment product; Its concrete steps are as follows:

A. sample pre-treatments: directly take sample 0.5g, adds several beaded glasses successively, 1% sodium sulfite solution 1mL, ethanol 12mL, 1% triethanolamine phosphate buffer (pH=7.7) 12mL; Concussion, makes sample disperse completely in the solution; Ultrasonic extraction 15min; With sample introduction analysis after 0.45 μm of organic membrane filtration;

B. standard reserving solution preparation: these four kinds of standard items ethanol of p-PDA HCl, P-amino-phenol, resorcinol, m-amino-phenol configure ,-18 DEG C of preservations; Toluene-2,4-diisocyanate, 5-diamines sulfate water-ethanol configures sample solution, regulates pH to be alkalescence, add sodium sulphite ,-18 DEG C of preservations with ammoniacal liquor;

C. standard working solution preparation: by standard reserving solution with 1% triethanolamine phosphate buffer (pH=7.7) be diluted to concentration be 0.5,1.0,2.5,5.0, the solution of 10.0ppm, now with the current;

D. analysis condition: SB C18 post (4.6 × 250mm, 5 μm), mobile phase: 1% triethanolamine phosphate buffer (pH=7.7)-acetonitrile=95: 5,1.5mL/min, room temperature is separated, and sample size 10 μ L, DAD detect, p-phenylenediamine and p-aminophenol determined wavelength is 305nm, and all the other are 280nm.

Embodiment recited above is only be described the preferred embodiment of the present invention, not limits the spirit and scope of the present invention.Under the prerequisite not departing from design concept of the present invention; the various modification that this area ordinary person makes technical scheme of the present invention and improvement; all should drop into protection scope of the present invention, the technology contents of request protection of the present invention, all records in detail in the claims.

Claims (1)

1. an oxidative hair dyes detection method in cosmetics, is characterized in that for p-PDA HCl, P-amino-phenol, Toluene-2,4-diisocyanate, the detection of 5-diamines sulfate, resorcinol, these 5 kinds of objects of m-amino-phenol, and this detection method needs 5 increment product; Its concrete steps are as follows:

A. sample pre-treatments: directly take sample 0.5g, adds several beaded glasses successively, 1% sodium sulfite solution 1mL, ethanol 12mL, 1% triethanolamine phosphate buffer (pH=7.7) 12mL; Concussion, makes sample disperse completely in the solution; Ultrasonic extraction 15min; With sample introduction analysis after 0.45 μm of organic membrane filtration;

B. standard reserving solution preparation: these four kinds of standard items ethanol of p-PDA HCl, P-amino-phenol, resorcinol, m-amino-phenol configure ,-18 DEG C of preservations; Toluene-2,4-diisocyanate, 5-diamines sulfate water-ethanol configures sample solution, regulates pH to be alkalescence, add sodium sulphite ,-18 DEG C of preservations with ammoniacal liquor;

C. standard working solution preparation: by standard reserving solution with 1% triethanolamine phosphate buffer (pH=7.7) be diluted to concentration be 0.5,1.0,2.5,5.0, the solution of 10.0ppm, now with the current;

D. analysis condition: SB C18 post (4.6 × 250mm, 5 μm), mobile phase: 1% triethanolamine phosphate buffer (pH=7.7)-acetonitrile=95: 5,1.5mL/min, room temperature is separated, and sample size 10 μ L, DAD detect, p-phenylenediamine and p-aminophenol determined wavelength is 305nm, and all the other are 280nm.