CN104777108B - The detection means and method of a kind of chlorophyll content - Google Patents
The detection means and method of a kind of chlorophyll content Download PDFInfo
- Publication number
- CN104777108B CN104777108B CN201510059807.7A CN201510059807A CN104777108B CN 104777108 B CN104777108 B CN 104777108B CN 201510059807 A CN201510059807 A CN 201510059807A CN 104777108 B CN104777108 B CN 104777108B
- Authority
- CN
- China
- Prior art keywords
- chlorophyll
- sample
- absorbance
- circuit
- tested
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Abstract
The invention discloses a kind of detection means of chlorophyll content and method, this method includes：Using the chlorophyll solution of various concentrations, chlorophyll detection model is established；Sample to be tested is detected using the incident light of two wavelength, obtains the absorbance of the chlorophyll of the sample to be tested；The absorbance of the chlorophyll of the sample to be tested is substituted into the chlorophyll detection model, obtains the concentration of the chlorophyll of the sample to be tested.This method is respectively 645nm with wavelength and 663nm light irradiation sample, the absorbance under corresponding wavelength can be obtained by the intensity of photoelectric sensor measurement transmitted light, so as to calculate the relative concentration values of chlorophyll.Major advantage is：Analyze speed is fast, measurement range is wide, preparation of samples is simple, does not consume sample, without chemical contamination etc..
Description
Technical field
The present invention relates to technical field of spectral detection, and in particular to the detection means and method of a kind of chlorophyll content.
Background technology
It as the saying goes：" breed fish and first support water ".Want to obtain aquaculture success, it is crucial that regulating and controlling water quality is handled properly.
And the key of regulating and controlling water quality is in aquaculture：Dissolved oxygen, transparency, acid-base value etc..Wherein dissolved oxygen amount is aquiculture animal
Biological element, chlorophyll content decides the dissolved oxygen amount in water body, and chlorophyll is the important composition composition of algae, chlorophyll
Content can also reflect water quality condition to a certain extent.
The method that water body chlorophyll content is measured with spectrophotometer 721 belongs to sampling analysis mensuration, common practice
It is that scene is taken and laboratory is taken back after water sample analyze measure, it is necessary to through several steps such as over-sampling, filtering, extraction, measure.
Such assay method accuracy of detection is higher, and can analyze constituent content.But the preprocessing process of sample is complicated, easily exists
Secondary pollution is brought in operating process；Sample takes back laboratory and needs the regular hour from marine or lake, and chlorophyll exists
In the presence of microorganism, it may be decomposed, it is difficult to accurately reflect the truth in ecosystem Determination of Chlorophyll.
Chlorophyll monitoring system using the type chlorophyll sensors of YSI 6025 as core.The type chlorophyll sensors of YSI 6025
Using living body measurement method, that is, the feature that make use of chlorophyll fluorescence to respond, fluorescence is discharged under specific wavelength light photograph, it is glimmering
Luminous intensity is proportional to chlorophyll concentration.The fluorescence intensity caused by exciting is very faint, therefore the detection method is in actual inspection
It is very high to environmental requirement in survey, easily disturbed by ambient light.Simultaneously because the uncertainty of water sample impurity, causes to examine
Surveying result also can be by a certain degree of interference.
The content of the invention
For in the prior art the defects of, the invention provides a kind of detection means of chlorophyll content and method, the party
Method analyze speed is fast, measurement range is wide.
In a first aspect, the present invention provides a kind of detection method of chlorophyll content, including：
Using the chlorophyll solution of various concentrations, chlorophyll detection model is established；
Sample to be tested is detected using the incident light of two wavelength, obtains the extinction of the chlorophyll of the sample to be tested
Degree；
The absorbance of the chlorophyll of the sample to be tested is substituted into the chlorophyll detection model, obtains the sample to be tested
Chlorophyll concentration.
Optionally, the chlorophyll solution using various concentrations, chlorophyll detection model is established, including：
The standard chlorophyll solution of various concentrations is detected using the light under two wavelength；
Absorbance and chlorophyll concentration corresponding to the light of described two wavelength are calculated respectively；
According to the absorbance and the chlorophyll concentration, chlorophyll detection model is established.
Optionally, described two wavelength are 663nm and 645nm.
Second aspect, present invention also offers a kind of detection means of chlorophyll content, including：Model building module, light
Electric detecting module and detection module, the model building module are arranged in the detection module, the photoelectric detection module with
The detection module is connected；
The model building module, for the chlorophyll solution using various concentrations, establish chlorophyll detection model；
The photoelectric detection module, for being detected using the incident light of two wavelength to sample to be tested, described in acquisition
The absorbance of the chlorophyll of sample to be tested；
The detection module, mould is detected for the absorbance of the chlorophyll of the sample to be tested to be substituted into the chlorophyll
Type, obtain the concentration of the chlorophyll of the sample to be tested.
Optionally, the model building module, is specifically included：
The standard chlorophyll solution of various concentrations is detected using the light under two wavelength；
Absorbance and chlorophyll concentration corresponding to the light of described two wavelength are calculated respectively；
According to the absorbance and the chlorophyll concentration, chlorophyll detection model is established.
Optionally, described two wavelength are 663nm and 645nm.
Optionally, the detection module includes：Voltage conversion circuit, light source current stabilization circuit, I-U change-over circuits, filtering are put
Big circuit, A/D change-over circuits, display circuit and storage circuit.
Optionally, the voltage conversion circuit, for being powered by voltage conversion to the detection module.
Optionally, the light source current stabilization circuit, for light source power supply.
Optionally, the storage circuit, the absorbance of the chlorophyll for storing the sample to be tested.
As shown from the above technical solution, the detection means and method of a kind of chlorophyll content provided by the invention, this method
With wavelength it is respectively 645nm and 663nm light irradiation sample, the intensity of transmitted light is measured by photoelectric sensor can obtain pair
The absorbance under wavelength is answered, so as to calculate the relative concentration values of chlorophyll.Major advantage is：Analyze speed is fast, measurement model
Enclose that wide, preparation of samples is simple, does not consume sample, without chemical contamination etc..
Brief description of the drawings
Fig. 1 is a kind of schematic flow sheet of the detection method for chlorophyll content that one embodiment of the invention provides；
Fig. 2 is the structure of the detecting device schematic diagram that one embodiment of the invention provides；
Fig. 3 is the light channel structure design diagram that another embodiment of the present invention provides；
Fig. 4 is the voltage conversion circuit schematic diagram that one embodiment of the invention provides；
Fig. 5 is the light source current stabilization power supply circuit schematic diagram that one embodiment of the invention provides；
Fig. 6 is the I-U change-over circuit schematic diagrames that one embodiment of the invention provides；
Fig. 7 is the filter amplification circuit schematic diagram that one embodiment of the invention provides；
Fig. 8 is the A/D change-over circuit schematic diagrames that one embodiment of the invention provides；
Fig. 9 is the display circuit schematic diagram that one embodiment of the invention provides；
Figure 10 is the serial port circuit and synchronous USB flash disk memory module schematic diagram that one embodiment of the invention provides；
Figure 11 is the Electric signal processing process schematic that one embodiment of the invention provides；
Figure 12 is the schematic flow sheet of one embodiment of the invention detection means detection；
Figure 13 is the measurement procedure schematic diagram that one embodiment of the invention provides；
Figure 14 is the USB flash disk Stored Procedure schematic diagram that one embodiment of the invention provides；
Embodiment
Below in conjunction with the accompanying drawings, the embodiment of invention is further described.Following examples are only used for more clear
Illustrate to Chu technical scheme, and can not be limited the scope of the invention with this.
Fig. 1 shows a kind of schematic flow sheet of the detection method of chlorophyll content provided in an embodiment of the present invention, such as Fig. 1
Shown, this method comprises the following steps：
101st, using the chlorophyll solution of various concentrations, chlorophyll detection model is established；
102nd, sample to be tested is detected using the incident light of two wavelength, obtains the chlorophyll of the sample to be tested
Absorbance；Described two wavelength are 663nm and 645nm.
103rd, the absorbance of the chlorophyll of the sample to be tested is substituted into the chlorophyll detection model, obtained described to be measured
The concentration of the chlorophyll of sample.
The above method is respectively 645nm with wavelength and 663nm light irradiation sample, and transmitted light is measured by photoelectric sensor
Intensity can obtain absorbance under corresponding wavelength, so as to calculate the relative concentration values of chlorophyll.Major advantage is：Point
Analysis speed is fast, measurement range is wide, preparation of samples is simple, does not consume sample, without chemical contamination etc..
The chlorophyll solution of various concentrations is utilized described in above-mentioned steps 101, establishes chlorophyll detection model, including：
1011st, the standard chlorophyll solution of various concentrations is detected using the light under two wavelength；
1012nd, absorbance and chlorophyll concentration corresponding to the light of described two wavelength are calculated respectively；
1013rd, according to the absorbance and the chlorophyll concentration, chlorophyll detection model is established.
The above method has the following advantages that：
(1) transmission principle is used, improves determination efficiency
Traditional chlorophyll detection method generally requires to carry out complicated sample preprocessing, and being carried plus instrument is inconvenient to lead
Cause sample to pass through a very long time from measurement is collected, cause the decomposition of chlorophyll, have impact on measurement accuracy.Nothing of the present invention
Sample preprocessing is needed, can now gather existing measurement.The integrality of sample is ensure that, improves measurement accuracy, and safely without dirt
Dye.
Chlorophyll content detection is carried out using transmission beam method principle, can avoid what is be likely to occur in sampling, preprocessing process
Phenomena such as chlorophyll degradation, other Substances Pollutions, so as to ensure that the accuracy of chlorophyll concentration detection.Due to fluorescent intensity compared with
It is weak, easily disturbed by ambient light when detecting, therefore the optical texture of instrument can be simplified using transmission beam method Cleaning Principle, together
When lowering apparatus environmental requirement when in use, so as to exploitation, making and the use cost of lowering apparatus, popularization to instrument and
Using advantageously.
(2) reliable high-precision water body chlorophyll content detection model is provided
For Accurate Prediction water body chlorophyll content, the present invention directly establishes and using the chlorophyll in spectrophotometric principle
Assay model.First with the standard chlorophyll solution of various concentrations, standard chlorophyll detection model is established.Utilize two
Light under wavelength detects to the standard chlorophyll solution of various concentrations, calculates corresponding absorbance.Survey is utilized afterwards
The absorbance and corresponding chlorophyll concentration obtained, the detection model (R2=0.84) of high-precision chlorophyll concentration is established, and will
The detection model is directly embedded into instrument.Model is demarcated and corrected first with chlorophyll titer during measurement, so
Uniform Sample is measured using the incident light of two different wave lengths afterwards, two are measured respectively using optical texture and independent circuits
The transmissivity of light under individual wavelength, chlorophyll content is finally calculated according to embedded water body chlorophyll concentration detection model.
(3) accuracy of detection is ensured from structure
In order to improve the accuracy of detector, influence of the particle movement to result in water is reduced, is distinguished using upper and lower two-way
The mode of collection measures chlorophyll content, and last chlorophyll content is drawn by averaging.This measuring method is by water sample
Influence of the particulate deposits to chlorophyll content is preferably minimized in microorganism uneven distribution and measurement process, improves measurement essence
Degree.
(4) data provided convenience support instrument
The present invention uses for measuring in real time, in order to preserve historical record and increase amount of storage, there is provided USB flash disk is synchronous
Store function, the needs repeatedly measured are met, facilitate the realization of the calling of data and water quality distribution map in the future.
Detection water body chlorophyll content is to preferably instruct aquaculture personnel to regulate and control water quality, improves production
Amount.
Fig. 2 shows a kind of detection means of chlorophyll content provided in an embodiment of the present invention, including：Model establishes mould
Block, photoelectric detection module and detection module, the model building module are arranged in the detection module, the photodetection mould
Block is connected with the detection module；
The model building module, for the chlorophyll solution using various concentrations, establish chlorophyll detection model；
The photoelectric detection module, for being detected using the incident light of two wavelength to sample to be tested, described in acquisition
The absorbance of the chlorophyll of sample to be tested；Described two wavelength are 663nm and 645nm.
The detection module, mould is detected for the absorbance of the chlorophyll of the sample to be tested to be substituted into the chlorophyll
Type, obtain the concentration of the chlorophyll of the sample to be tested.
The model building module, is specifically included：
The standard chlorophyll solution of various concentrations is detected using the light under two wavelength；
Absorbance and chlorophyll concentration corresponding to the light of described two wavelength are calculated respectively；
According to the absorbance and the chlorophyll concentration, chlorophyll detection model is established.
According to Lambert-Beer laws, under certain condition the absorbance D of certain colored solutions and wherein solute concentration C and
Liquid layer thickness L is directly proportional, i.e.,：In D=aCL formulas, a is proportionality constant.If containing several extinction materials in solution, this mixing
Absorbance of the liquid under a certain wavelength be equal to each component absorbance under respective wavelength summation, here it is absorbance plus and
Property.If determining chlorophyll content, the absorbance A of the water sample at a particular wavelength only need to be determined, and according to chlorophyll a, b in the ripple
Specific absorptivity under long can obtain its concentration.It is used when determining chlorophyll a, b in order to exclude the interference of carotenoid
Maximum absorption band of the monochromatic wavelength selection chlorophyll at feux rouges.Maximum absorption band of the known chlorophyll a, b in red light district
Respectively 663nm and 645nm, it is 663nm and 645nm because choosing sensitive band.
It is A=lg [I for the absorbance calculation formula under two wave bandss(λ)/Iz(λ)], wherein A is absorbance, Is(λ)
For the transmission value of titer (distilled water), Iz(λ) is the transmission of water sample.Maximum absorption band of the known chlorophyll a, b in red light district
Respectively 663nm and 645nm, know again under wavelength 663nm, specific absorptivity of the chlorophyll a, b in the solution is respectively
82.04 and 9.27, it is respectively 16.75 and 45.6 under wavelength 645nm, relationship below is listed according to additive property principle：
A663=82.04Ca+9.27Cb A645=16.75Ca+45.6Cb
A663 and A645 is respectively absorbance of the chlorophyll solution in wavelength 663nm and 645nm in formula, and Ca, Cb distinguish
For the concentration of chlorophyll a, b, unit mg/L.Solving equations can obtain：
Ca=12.72A663-2.59A645 Cb=22.88A645-4.67A663
Ca, Cb are respectively the concentration of chlorophyll a, b in formula, unit mg/L.Ca is added with Cb to produce chlorophyll always dense
Spend (CT, mg/L):CT=Ca+Cb=20.29A645+8.05A663
It will be located in model insertion single-chip microcomputer in detection means, for being directly invoked during measurement.
Detection means is made up of opticator and control section.Opticator includes 4 LED light sources transmitting of 2 wave bands
Device, it is powered by monolithic processor controlled light source driving circuit, is impinged perpendicularly on after collimation lens is collimated in water,
Gathered accordingly by 4 photodetectors；Control section includes amplifying circuit, filter circuit, A/D change-over circuits, monolithic
Machine control circuit, liquid crystal display circuit and synchronous USB flash disk storage circuit.Detector is lighted successively using single-chip microcomputer control light source,
The single band optical signal that light source is sent is impinged perpendicularly in sample after collimation lens to be measured, by the absorption of sample,
Scattering and transmission, are acquired, optical signal is converted into electric signal by photodetector by corresponding photodetector.Visited through photoelectricity
The electric signal that device is converted into is surveyed to be kept in by 89S52 single-chip microcomputers after the processing such as amplification, filtering, A/D conversions, and with receiving successively
The optical signal come in calculates to be substituted into water body chlorophyll content detection model after absorbance and is calculated together, the result after calculating
Exported by 128*64 liquid crystal displays, while the functions such as USB flash disk storage can be realized.Fig. 2 is system global structure design drawing.
The detection module includes：Voltage conversion circuit, light source current stabilization circuit, I-U change-over circuits, filter amplification circuit,
A/D change-over circuits, display circuit and storage circuit.
Sensor main will be responsible for the driving of light source, the collection of optical signal, the processing of photoelectric conversion and electric signal, amplification,
The work such as collection, transmission.Fig. 2 is the general structure block diagram of sensor.Below will respectively from mechanical structure, hardware circuit design,
Software circuit design etc. describes in detail.
A, sensor mechanical structure designs
A, Fundamentals of Sensors design
Chlorophyll is one of important composition composition of algae, is the important factor for carrying out algae light and effect.Chlorophyll contains
The height of amount is relevant with quality of water environment, directly determines the oxygen content height of water body.Chlorophyll has special optical characteristics.
Most of chlorophyll a and whole chlorophyll bs have the function that to absorb luminous energy, but the leaf of only only a few special state is green
Plain a molecules, just have the function that to convert light energy into electric energy.Therefore we only need according to absorption of the chlorophyll to visible spectrum
Absorbance A of the sample under two specific wavelengths is determined using survey meter, and according to chlorophyll a, b and carotenoid at this
Specific absorptivity under wavelength can obtain its concentration.When determining chlorophyll a, b in order to exclude the interference of carotenoid, institute
With maximum absorption band of the monochromatic wavelength selection chlorophyll at feux rouges.It is added i.e. after obtaining the concentration of chlorophyll a, b respectively
Obtain chlorophyll total concentration.
B, light path system designs
Light path system mainly completes the transmission of optical signal by collimation lens, and optical signal is completed by photodetector
Receive, be mainly made up of two parts, optical signal transmission path and receiving portion.
For the accuracy of detection, it is necessary to select suitable light source and its acquisition mode.According to low-power consumption, narrow bandwidth, just
The principle of formula and the principle of detector are taken, selects the light emitting diode of 2 different-wavebands as incident light source, collimation lens conduct
Transmission channel.Light path design after the collimated lens of light emitting diode as shown in figure 3, impinge perpendicularly in the water sample of sample bottle, warp
Gathered after crossing the absorption, scattering and transmission of water sample by corresponding sensor to complete transformation of the optical signal to electric signal.This detection
Instrument the upper and lower are same structure.
Single-chip microcomputer controls lightening LED lamp 1,2,3,4 successively, respectively by the acquisition of transmission signal of detector 1,2,3,4, adopts every time
Corresponding lamp lights next lamp again after going out after having collected.
C, sensor hardware circuit design
Instrument is powered by a 9V battery after power circuit is changed to each circuit, and four LED light sources are by constant-current source list
Solely power supply.Optical signal is changed into after electric signal, it is necessary to which the progress Electric signal processing work of a series of hardware circuit, mainly includes
I-U change-over circuits, filter amplification circuit, A/D change-over circuits, liquid crystal display circuit and USB flash disk storage circuit.
(1) voltage conversion circuit and light source current stabilization circuit
Power supply used in portable detector is 9 volts of dry cells, and the operational voltage value of each chip is 5 volts in circuit, this
Power-switching circuit is needed to realize.L7805 chips are used to be used for the 9V photovoltaic conversions of battery as 5V chip power supply voltages, figure
4 be voltage conversion circuit figure.The voltage conversion circuit, for being powered by voltage conversion to the detection module.
Light source intensity and spectral ripple can be caused because extraneous power supply is unstable, it should to LED light source using constant-current source electricity
Road powers.Such as Fig. 5, constant current-supplying circuit is formed with LM317.Wherein LM317 input end groundings, according to output end and control terminal it
Between formed 1.25V burning voltages principle, form current stabilization source circuit and provide constant-current source for LED.The light source current stabilization circuit, is used for
To light source power supply.
(2) I-U change-over circuits
Signal is sent by light emitting diode used by due to the system, is transmitted by collimation lens, and signal is fainter,
The electric signal obtained again after the conversion of InGaAs photodetectors is fainter, in addition, being also mingled with this process each
The interference of kind noise, therefore it is the I- realized by CA3140 to have selected the integrated operational amplifier CA3140, Fig. 6 of high input impedance
U change-over circuits, while realizing I-U conversions, realize an enlarging function of small-signal.
(3) filter amplification circuit
The secondary enlarging function of small-signal is realized by LM358, is put signal by the resistance for adjusting R9 potentiometers
It is big 380 times, signal is sent into by AD7705 by the low-pass first order filter of R-C compositions and carries out A/D conversions.
(4) A/D change-over circuits
The analog signal amplified twice is changed into data signal by Fig. 8 A/D change-over circuits, is sent into 89S52 single-chip microcomputer
System is handled, and A/D change-over circuits are mainly serially defeated by a 16 bit resolution, three lines of 2 passage fully differential simulation inputs
The analog-digital converter AD7705 chips entered are realized.
AD7705 can be easily connected with microcontroller, also be compared using 3-line serial interface compatible SP I Q SP I
Parallel interface mode greatlys save CPU I O mouths.AD7705 is complete 16 bit A/D converter.It employs sigma-delta skill
Art, 16 can be obtained without number of bit errors according to output.This point meet very much it is higher to resolution requirement but to change numerical requirements
Not high application, pass through external crystal oscillator, accurate a reference source and a small amount of decoupling capacitor, you can be carried out continuously A D conversions.It is logical
Cross and be connected with REF195 and reference voltage can be made to bring up to 5V, reach requirement.
(5) liquid crystal display circuit
Liquid crystal display circuit uses QC12864B Chinese character image dot matrix lcd modules, can Display of Chinese characters and figure, it is interior
8192 Chinese characters (16*16 dot matrix), 128 characters (8*16 dot matrix) and 62*256 lattice display RAM64 dot matrix are put, point is big
Small 0.48*0.48mm2, point spacing 0.04mm.This circuit design uses serial line interface, by being connected with single-chip microcomputer, realizes effective
Control the correct display of liquid crystal display.Fig. 9 is liquid crystal display circuit figure.
(6) synchronous USB flash disk storage circuit
The storage circuit, the absorbance of the chlorophyll for storing the sample to be tested.What USB flash disk storage was selected is Nanjing
The USB flash disk module of Qin Heng electronics corporations production is realized between single-chip microcomputer and memory module as storage device by MAX232 chips
Communication.Figure 10 is serial port circuit and synchronous USB flash disk memory module.
Current signal I0 after being changed through photodetector, by I-U change-over circuits, current signal I0 is changed into voltage
Signal U0, by the amplifying circuit being made up of LM358, voltage signal U0 is changed into U1, and some noise signals are now included in signal
△ U, by the low-pass first order filter being made up of RC, noise signal △ U are removed, obtain U2 (U1- △ U).
Analog signal U2 is changed into data signal U3 by A/D change-over circuits, and data signal U3 is sent into single-chip microcomputer 89S52 processing
Handled in device, final result is shown on 12864B liquid crystal displays, while is stored in by USB flash disk memory module in USB flash disk.Figure 11 is
Electric signal processing process.
C, operational process designs
Software program, which is adopted, to be shown a C language, and mainly realizes light source control function, keyboard identification function, A/D translation functions,
Software filtering function, data processing function, crystal display and USB flash disk store function, figure are main program flow chart, measurement procedure
Figure and USB flash disk storage program flow chart.
The present invention's is simple to operate, is detector detailed operation and data processing method below.
1st, detection means job step
The first step, press【Power supply】Key, open system power supply, liquid crystal display：" in China Agricultural University's precision agriculture research
Edema with the heart involved body chlorophyll content detector ", instrument initialized completion is represented, can be with normal work.
Second step, the sampling bottle of distilled water will be filled, be put into circular groove, press【Demarcation】Key, system will gather four successively
Individual light intensity value, and it is included on liquid crystal display, the data collected are saved in USB flash disk by system automatically by Serial Port Transmission,
Liquid crystal display " in data storage ".After the completion of data transfer, interface display " storage is completed ".If do not plug USB flash disk or USB flash disk does not have
It is plugged, interface can prompt " please insert USB flash disk ".
3rd step, the sampling bottle of water sample will be filled, be put into circular groove, press【Measurement】Key, system will gather four successively
Light intensity value, and it is included on liquid crystal display, it is automatically saved in USB flash disk.
4th step, after the light intensity value for collecting standard solvent and test solvent, its absorbance of system-computed and according to
There is chlorophyll content forecast model, calculate the chlorophyll content of water sample, be shown on liquid crystal display and be saved in USB flash disk.
5th step, is reentered into sample, presses【Measurement】Key measures.
2 chlorophyll levels distribution map implementation methods
Water body chlorophyll content distribution figure is realized using VC method, can sit single-point water body chlorophyll content and GPS
Mark information to combine, realize that single-point water body chlorophyll content is shown, interpolation, plot area measuring and calculating, cell water body chlorophyll content etc.
It is worth line chart generation, decision-making foundation is provided for regulating and controlling water quality.
Said apparatus has advantages below：
1st, the in situ detection of chlorophyll concentration is realized
Using transmission beam method Cleaning Principle, the steps such as sampling in chlorophyll detection, pretreatment, purification are eliminated, at the same it is sharp
Measure the mode of averaging respectively with upper and lower two-way, can effectively realize the in situ detection of water body Determination of Chlorophyll concentration.To water
Water quality in production cultivation plays effective monitoring and directive function.
2nd, the change of monitoring chlorophyll concentration in real time
With reference to upper computer software, the chlorophyll concentration distribution map of different zones in waters can be generated in real time.In combination with
Other meteorological and hydrographic informations, change and the trend of water body Determination of Chlorophyll concentration can be effectively predicted, so as to aquaculture
Play positive directive function.
3rd, it is practical
The chlorophyll concentration distribution map that the present invention is generated by LCD screen and upper computer software can intuitively reflect water outlet
Chlorophyll distribution in body region, effectively can intuitively carry out monitoring water quality and carry out regulating and controlling water quality.Can have for regulating and controlling water quality
The offer decision-making foundation of effect.
In the specification of the present invention, numerous specific details are set forth.It is to be appreciated, however, that embodiments of the invention can be with
Put into practice in the case of these no details.In some instances, known method, structure and skill is not been shown in detail
Art, so as not to obscure the understanding of this description.
Finally it should be noted that：Various embodiments above is merely illustrative of the technical solution of the present invention, rather than its limitations；To the greatest extent
The present invention is described in detail with reference to foregoing embodiments for pipe, it will be understood by those within the art that：Its according to
The technical scheme described in foregoing embodiments can so be modified, either which part or all technical characteristic are entered
Row equivalent substitution；And these modifications or replacement, the essence of appropriate technical solution is departed from various embodiments of the present invention technology
The scope of scheme, it all should cover among the claim of the present invention and the scope of specification.
Claims (5)
- A kind of 1. detection method of chlorophyll content, it is characterised in that including：Using the chlorophyll solution of various concentrations, establish Chlorophyll detection model；Sample to be tested is detected using the incident light of two wavelength, obtains the absorbance of the chlorophyll of the sample to be tested, Described two wavelength are 663nm and 645nm；The absorbance of the chlorophyll of the sample to be tested is substituted into the chlorophyll detection model, obtains the leaf of the sample to be tested Green plain concentration：It is A=lg [I for the absorbance calculation formula under two wave bandss(λ)/Iz(λ)], wherein A is absorbance, Is (λ) be titer transmission value, Iz(λ) is the transmission of water sample；Maximum absorption band of the known chlorophyll a, b in red light district be respectively 663nm and 645nm, know again under wavelength 663nm, specific absorptivity of the chlorophyll a, b in the solution is respectively 82.04 Hes 9.27, it is respectively 16.75 and 45.6 under wavelength 645nm, relationship below is listed according to additive property principle：A663=82.04Ca+9.27Cb A645=16.75Ca+45.6CbA663 and A645 is respectively absorbance of the chlorophyll solution in wavelength 663nm and 645nm in formula, and Ca, Cb are respectively leaf Green plain a, b concentration, unit mg/L；Solving equations can obtain：Ca=12.72A663-2.59A645 Cb=22.88A645-4.67A663Ca, Cb are respectively the concentration of chlorophyll a, b in formula, unit mg/L；Ca is added with Cb and produces chlorophyll total concentrationCT=Ca+Cb=20.29A645+8.05A663,It will be located in model insertion single-chip microcomputer in detection means, for being directly invoked during measurement；Detection means is made up of opticator and control section, and opticator includes 4 LED light source emitters of 2 wave bands, It is powered by monolithic processor controlled light source driving circuit, is impinged perpendicularly on after collimation lens is collimated in water, by 4 Photodetector is gathered accordingly；Control section includes amplifying circuit, filter circuit, A/D change-over circuits, single-chip microcomputer control Circuit, liquid crystal display circuit and synchronous USB flash disk storage circuit；Detector is lighted successively using single-chip microcomputer control light source, light source hair The single band optical signal gone out is impinged perpendicularly in sample after collimation lens to be measured, by the absorption of sample, scattering and Transmission, is acquired, optical signal is converted into electric signal by photodetector by corresponding photodetector；Turn through photodetector The electric signal changed into is by amplifying, filtering, is kept in after A/D conversion process by 89S52 single-chip microcomputers, and the light with receiving successively Signal of change, which goes out after absorbance to substitute into water body chlorophyll content detection model together, to be calculated, and the result after calculating is by 128*64 Liquid crystal display exports, while realizes USB flash disk store function；According to low-power consumption, narrow bandwidth, the principle of portable principle and detector, the light emitting diodes of 2 different-wavebands is selected As incident light source, collimation lens impinges perpendicularly on the water of sample bottle as transmission channel after the collimated lens of light emitting diode In sample, gathered after the absorption, scattering and transmission of water sample by corresponding sensor to complete transformation of the optical signal to electric signal, This detector the upper and lower are same structure；The device that the detection method of the chlorophyll content uses includes：Model building module, photoelectric detection module and detection mould Block, the model building module are arranged in the detection module, and the photoelectric detection module is connected with the detection module；The model building module, for the chlorophyll solution using various concentrations, establish chlorophyll detection model；The photoelectric detection module, for being detected using the incident light of two wavelength to sample to be tested, obtain described to be measured The absorbance of the chlorophyll of sample；The detection module, for the absorbance of the chlorophyll of the sample to be tested to be substituted into the chlorophyll detection model, obtain Take the concentration of the chlorophyll of the sample to be tested.
- 2. according to the method for claim 1, it is characterised in that the detection module includes：Voltage conversion circuit, light source are steady Current circuit, I-U change-over circuits, filter amplification circuit, A/D change-over circuits, display circuit and storage circuit.
- 3. according to the method for claim 2, it is characterised in that the voltage conversion circuit, for being given by voltage conversion The detection module power supply.
- 4. according to the method for claim 2, it is characterised in that the light source current stabilization circuit, for light source power supply.
- 5. according to the method for claim 2, it is characterised in that the storage circuit, for storing the sample to be tested The absorbance of chlorophyll.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510059807.7A CN104777108B (en) | 2015-02-04 | 2015-02-04 | The detection means and method of a kind of chlorophyll content |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510059807.7A CN104777108B (en) | 2015-02-04 | 2015-02-04 | The detection means and method of a kind of chlorophyll content |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104777108A CN104777108A (en) | 2015-07-15 |
| CN104777108B true CN104777108B (en) | 2018-01-19 |
Family
ID=53618715
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510059807.7A Active CN104777108B (en) | 2015-02-04 | 2015-02-04 | The detection means and method of a kind of chlorophyll content |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104777108B (en) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106248591B (en) * | 2016-08-10 | 2022-03-04 | 中国农业科学院烟草研究所 | Device for distinguishing maturity degree of tobacco leaves and distinguishing method thereof |
| CN107228843B (en) * | 2017-05-08 | 2019-05-24 | 绍兴市水环境科学研究院有限公司 | A kind of calibration method applied to fluorescence method chlorophyll a on-line computing model |
| GB2566064A (en) * | 2017-09-01 | 2019-03-06 | Plymouth Marine Laboratory | Chlorophyll absorption meter |
| CN108872108A (en) * | 2018-07-02 | 2018-11-23 | 湖北省农业科学院中药材研究所 | Plant young leaflet tablet pigment detection method |
| CN109238993A (en) * | 2018-11-28 | 2019-01-18 | 南昌航空大学 | The detection method that Determination of Chlorophyll In Seawater content influences underwater optical transmission characteristics |
| WO2021010817A1 (en) | 2019-07-12 | 2021-01-21 | Sime Darby Plantation Intellectual Property Sdn. Bhd. | Apparatus to measure ripeness of oil palm fruitlets via real-time chlorophyll content measurement |
| CN115436307B (en) * | 2022-09-16 | 2023-05-16 | 陕西汉谱迅光电科技有限公司 | Method for nondestructively and rapidly detecting chlorophyll and carotenoid content in plant leaves |
| CN116660206A (en) * | 2023-05-31 | 2023-08-29 | 浙江省农业科学院 | Crop yield estimation method and system |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101655462A (en) * | 2009-09-11 | 2010-02-24 | 中国科学院地理科学与资源研究所 | Apparatus for obtaining water quality information, method and system for recognizing water body eutrophication degree |
| CN103302086A (en) * | 2013-06-03 | 2013-09-18 | 贵州师范大学 | Remediation method of karst mountain mine soil subjected to heavy metal copper pollution |
| CN103353439A (en) * | 2013-07-05 | 2013-10-16 | 浙江大学 | Method for detecting chlorophyll content and biomass of chlorella based on spectrum technology |
| CN103499539A (en) * | 2013-09-25 | 2014-01-08 | 中国农业大学 | Aquaculture turbidity detector and aquaculture turbidity detection method based on optical theory |
-
2015
- 2015-02-04 CN CN201510059807.7A patent/CN104777108B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101655462A (en) * | 2009-09-11 | 2010-02-24 | 中国科学院地理科学与资源研究所 | Apparatus for obtaining water quality information, method and system for recognizing water body eutrophication degree |
| CN103302086A (en) * | 2013-06-03 | 2013-09-18 | 贵州师范大学 | Remediation method of karst mountain mine soil subjected to heavy metal copper pollution |
| CN103353439A (en) * | 2013-07-05 | 2013-10-16 | 浙江大学 | Method for detecting chlorophyll content and biomass of chlorella based on spectrum technology |
| CN103499539A (en) * | 2013-09-25 | 2014-01-08 | 中国农业大学 | Aquaculture turbidity detector and aquaculture turbidity detection method based on optical theory |
Non-Patent Citations (2)
| Title |
|---|
| 11种观赏地被植物引种栽培和耐荫性试验;朱云华等;《金陵科技学院学报》;20070630;第23卷(第02期);第1.3.2节 * |
| 缘管浒苔和羽藻氮、磷营养生理学研究;邵魁双等;《海洋学报》;20110531;第33卷(第3期);第2.5节 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104777108A (en) | 2015-07-15 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104777108B (en) | The detection means and method of a kind of chlorophyll content | |
| CN101975764B (en) | Near infrared spectrum technology-based multiband soil nitrogen detection device and method | |
| CN106198424B (en) | Full-spectrum-based water quality online monitoring device and monitoring method thereof | |
| CN104596990B (en) | Double channel optical fiber turbidimetry method and sensor | |
| CN103499539B (en) | Aquaculture turbidity survey meter and method based on optical principle | |
| CN101556240B (en) | Chlorophyll measuring apparatus based on RGB (red, green and blue) three-primary colors digital signals | |
| CN102519916B (en) | Method and device for on-line detecting concentration of pesticide | |
| CN202939121U (en) | Comprehensive collecting and processing system based on optical characteristics of water body | |
| CN104165853A (en) | Spectroscopy water environment online measuring device | |
| CN103698287A (en) | Dim light signal detection device for detecting pH value of seawater | |
| CN104568946A (en) | Intelligent optical fiber pH sensor | |
| CN104155247B (en) | Water body chlorophyll and turbidity in-situ determination method and determinator | |
| CN101556245B (en) | Chlorophyll measurement method based on RGB digital signal | |
| CN103149180B (en) | Detection method of soil spectral reflectivity and specific conductance | |
| CN105784606A (en) | Optical property based water quality monitoring system | |
| CN105717048A (en) | Water collection and treatment system based on aquaculture water optical characteristics | |
| CN109142224A (en) | A kind of intelligent portable ammonia nitrogen detector | |
| CN205404410U (en) | Double -light -path method littoral zone water chlorophyll normal position monitoring devices | |
| CN204439543U (en) | The main component content measuring system of a kind of emulsion | |
| CN209460124U (en) | A kind of intelligent portable ammonia nitrogen detector | |
| CN205484011U (en) | Processing system is gathered to quality of water based on aquaculture water body optical characteristic | |
| CN206627629U (en) | A kind of simple fish finder based on PSD | |
| CN207439918U (en) | Ocean color monitor | |
| CN105954203A (en) | Amplifying circuit-based optical property water quality monitoring system | |
| CN103822887B (en) | A kind of detection method of laser detection nitrite anions concentration |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| EXSB | Decision made by sipo to initiate substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |