CN109238993A - The detection method that Determination of Chlorophyll In Seawater content influences underwater optical transmission characteristics - Google Patents
The detection method that Determination of Chlorophyll In Seawater content influences underwater optical transmission characteristics Download PDFInfo
- Publication number
- CN109238993A CN109238993A CN201811430223.6A CN201811430223A CN109238993A CN 109238993 A CN109238993 A CN 109238993A CN 201811430223 A CN201811430223 A CN 201811430223A CN 109238993 A CN109238993 A CN 109238993A
- Authority
- CN
- China
- Prior art keywords
- chlorophyll
- seawater
- sample
- optical transmission
- transmission characteristics
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/3103—Atomic absorption analysis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/38—Diluting, dispersing or mixing samples
Landscapes
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
Abstract
The invention discloses a kind of detection methods that Determination of Chlorophyll In Seawater content influences underwater optical transmission characteristics, sample, spectral detection, spectrum analysis are prepared, compares the intensity of the absorption spectra of the full sea water sample and the seawater sample and the standard absorption peak of transmission spectrum and transmission peaks respectively;The average value for taking spectrum analysis calculates chlorophyll content to the absorptivity and transmissivity of spectrum.Present invention employs the methods of UV, visible light near infrared spectrometer, the absorption spectra and transmission spectrum of sample are measured respectively, the spectral characteristic for analyzing without chlorophyll seawater sample solution and being added chlorophyll seawater sample solution, to obtain influence of the Determination of Chlorophyll In Seawater content for underwater optical transmission characteristics.Accuracy is high, and effect is obvious, chlorophyll content can be precisely calculated to the absorptivity and transmissivity of spectrum, can detect Determination of Chlorophyll In Seawater content influences underwater optical transmission characteristics.
Description
Technical field
The present invention relates to spectrographic detection technology, in particular to a kind of Determination of Chlorophyll In Seawater content is for underwater optical transmission characteristics
The detection method of influence.
Background technique
Currently, seawater pollution problem, than more serious, seawater muddiness caused by marine pollution seriously affects sea-plant and (swims
Plant and seaweed) photosynthesis, to influence the productivity in sea area.Due to containing a large amount of phytoplanktons in seawater, they are
The primary product of light-use.The chlorophyll wherein contained can convert light energy into biological energy source, this is to influence light in seawater
One essential condition of absorption characteristic, so, the content of chlorophyll has a very big impact underwater light propagation.Due to chlorophyll
The scattering of light can be influenced, someone uses the experiment proves that had an impact Phytoplankton Photosynthesis In The Pohai and chlorophyll concentration
The factor can all influence primary productivity of marine ecosystem, so, one kind probing into Determination of Chlorophyll In Seawater content for underwater optical transmission characteristics shadow
Loud method is to get a good eye value.
Currently, there are many influence factor that influence light transmits in the seawater, ocean temperature, salinity, pressure, turbulent flow etc., still
Influence of the content to optical transport for phytoplankton in seawater, especially the content of simulated seawater Determination of Chlorophyll is for optical transport
Influence really rarely have research.Absorption of the chlorophyll content to 200-1200nm range of spectra under effective simulation real sea water environment
With scattering situation, to optical transport in seawater, optic communication has far-reaching influence.
Summary of the invention
Probe into what Determination of Chlorophyll In Seawater content influenced underwater optical transmission characteristics the purpose of the present invention is to provide a kind of
Method analyzes influence of the different depth chlorophyll content to optical transmission characteristics in seawater by optical spectrum detecting method.
The purpose of the present invention is realized using following technical scheme.Determination of Chlorophyll In Seawater content is for underwater optical transmission characteristics
The detection method of influence, its step are as follows:
1) it prepares sample: taking full sea water sample in cuvette, various concentration chlorophyll is respectively configured according to sea water advanced
Seawater sample;
2) spectrum inspection spectral detection: is carried out to the seawater sample using UV, visible light near infrared spectrometer
It surveys;
3) spectrum analysis: standard absorption peak and the transmission of the full sea water sample and the seawater sample are compared respectively
The intensity at peak, if identical, the chlorophyll content in seawater is on underwater optical transmission characteristics without influence;If it is different, then seawater
In chlorophyll content have an impact to underwater optical transmission characteristics;
4) average value for taking step 3) spectrum analysis calculates chlorophyll content to the absorptivity and transmissivity of spectrum.
Further, the salinity of the full sea water sample is 3~4%;
Further, it is 3.7mg/m that the chlorophyll content configuration of the seawater sample, which is respectively as follows: seawater surface,3;10 meters
Deep-sea water is 6.72mg/m3;15 meters of deep-sea water are 5.5mg/m3;20 meters of deep-sea water are 3mg/m3;
Further, the gap of the spectrographic detection is 7~9nm;
Further, the range of the spectrographic detection wavelength is 200nm-1400nm.
Present invention employs the methods of spectrophotometer, configure achlorophyllous seawater standard sample solution to be measured and addition
The seawater sample standard solution of various concentration chlorophyll measures the absorption spectra and transmission spectrum of sample respectively, and analysis is without chlorophyll sea
Sample solutions and the spectral characteristic that chlorophyll seawater sample solution is added, to obtain Determination of Chlorophyll In Seawater content for underwater
The influence of optical transmission characteristics.It needs to be repeated as many times in test to reduce error in the method being averaged, has studied chlorophyll and contain
The scattering and absorption to the light of 200nm-1400nm spectrum are measured, experimental spectrum range is wide, and accuracy is high, and effect is obvious, can be accurate
Ground calculates chlorophyll content to the absorptivity and transmissivity of spectrum, can detect Determination of Chlorophyll In Seawater content and Underwater Optical is passed
Broadcast characteristic influence.
Detailed description of the invention
Fig. 1 is detection device schematic diagram of the invention;
In figure: 100- UV, visible light near infrared spectrometer, 1-LED light source, the first convex lens of 2-, 3- pinhole filter
Device, 4- concavees lens, 5- slit, the second convex lens of 6-, 7- cuvette;8- signal receiving processing system, 9- computer.
Specific embodiment
Below in conjunction with drawings and examples, the invention will be further described.Referring to Fig. 1, Determination of Chlorophyll In Seawater of the present invention contains
UV, visible light near infrared spectrometer 100 in the detection method for influencing underwater optical transmission characteristics is measured to be issued by LED light source 1
Light beam passes through the collimation filtering system being made of the first convex lens 2, pinhole filter 3 and concavees lens 4, becomes directional light entrance point
Light grating slit 5 enters in article colorimetric ware 7 using the light beam for being divided into different wave length after the second convex lens 6, and compares
Color ware 7 passes through 9 connection signal receiving processing system 8 of computer.
Its testing procedure is as follows:
1. sample preparation configures the seawater standard solution of 3.5% salinity with sea salt first, then configuration and sample to be tested salt
Seawater sample that is identical but having added various concentration chlorophyll is spent, is then measured with UV, visible light near infrared spectrometer to test sample
The absorption spectra and transmission spectrum of product, the absorption peak and transmission peaks and Strength Changes situation of analysis absorption spectrum and projection spectrum.
2. no chlorophyll seawater sample solution salinity is 3.5%, the seawater sample simulation In The Western Part of The East China Sea edge of chlorophyll is added
It is green to be arranged respectively at addition leaf in 3.5% salinity seawater standard solution for the chlorophyll depth concentration levels of the true water body in bank area
Plain concentration is seawater surface: 3.7mg/m3;10 meters of deep-sea water: 6.72mg/m3;15 meters of deep-sea water: 5.5mg/m3;20 meters of deep-sea water:
3mg/m3These four concentration.
3. using LED light source 1, the first convex lens 2, pinhole filter 3, concavees lens 4, the slit 5, second set gradually
The UV, visible light near infrared spectrometer 100 that convex lens 6 and cuvette 7 are constituted, wherein cuvette 7 passes through computer 9 and letter again
Number receiving processing system 8 connects, and computer 9 uses UVProbe2.33 software.
4. firstly, the absorption spectra and transmission of seawater sample solution of the comparison without chlorophyll seawater sample and containing chlorophyll
Spectrum, if it is identical, then the standard absorption peak and transmission peaks of absorption spectra and transmission spectrum and the intensity at peak are compared, if identical,
Chlorophyll content in seawater is on underwater optical transmission characteristics without influence.If it is different, then the chlorophyll content in seawater is to underwater
Optical transmission characteristics have an impact.
The operation principle of the present invention is that: configuration sample to be tested opens computer 9 and UV, visible light near infrared spectrometer
100 work normally;Configure the seawater sample without chlorophyll seawater sample and containing various concentration chlorophyll;It is close by UV, visible light
Infrared spectrophotometer 100 measures absorption spectra and transmission spectrum and Strength Changes;It analyzes absorption spectrum and projects the absorption of spectrum
Peak and transmission peaks and Strength Changes situation obtain the detection comparing result of sample detection.
Claims (5)
1. the detection method that Determination of Chlorophyll In Seawater content influences underwater optical transmission characteristics, which is characterized in that its step are as follows:
1) it prepares sample: taking full sea water sample in cuvette, according to the sea water advanced sea that various concentration chlorophyll is respectively configured
Water sample;
2) spectral detection spectral detection: is carried out to the seawater sample using UV, visible light near infrared spectrometer;
3) spectrum analysis: the standard absorption peak and transmission peaks of the full sea water sample and the seawater sample are compared respectively
Intensity, if identical, the chlorophyll content in seawater is on underwater optical transmission characteristics without influence;If it is different, then in seawater
Chlorophyll content has an impact to underwater optical transmission characteristics;
4) average value for taking step 3) spectrum analysis calculates chlorophyll content to the absorptivity and transmissivity of spectrum.
2. the detection method that Determination of Chlorophyll In Seawater content according to claim 1 influences underwater optical transmission characteristics,
It is characterized in that, the salinity of the full sea water sample is 3~4%.
3. the detection method that Determination of Chlorophyll In Seawater content according to claim 1 influences underwater optical transmission characteristics,
It is characterized in that, it is 3.7mg/m that the chlorophyll content configuration of the seawater sample, which is respectively as follows: seawater surface,3;10 meters of deep-sea water
For 6.72mg/m3;15 meters of deep-sea water are 5.5mg/m3;20 meters of deep-sea water are 3mg/m3。
4. the detection method that Determination of Chlorophyll In Seawater content according to claim 1 influences underwater optical transmission characteristics,
It is characterized in that, the gap of the spectrographic detection is 7~9nm.
5. the detection method that Determination of Chlorophyll In Seawater content according to claim 1 influences underwater optical transmission characteristics,
It is characterized in that, the range of the spectrographic detection wavelength is 200nm-1400nm.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201811430223.6A CN109238993A (en) | 2018-11-28 | 2018-11-28 | The detection method that Determination of Chlorophyll In Seawater content influences underwater optical transmission characteristics |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201811430223.6A CN109238993A (en) | 2018-11-28 | 2018-11-28 | The detection method that Determination of Chlorophyll In Seawater content influences underwater optical transmission characteristics |
Publications (1)
Publication Number | Publication Date |
---|---|
CN109238993A true CN109238993A (en) | 2019-01-18 |
Family
ID=65074225
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201811430223.6A Pending CN109238993A (en) | 2018-11-28 | 2018-11-28 | The detection method that Determination of Chlorophyll In Seawater content influences underwater optical transmission characteristics |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN109238993A (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN113746544A (en) * | 2021-09-09 | 2021-12-03 | 桂林电子科技大学 | Vertical channel link layering method for non-uniform seawater |
Citations (14)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0915338A2 (en) * | 1997-11-10 | 1999-05-12 | Jeacle Limited | Photometric analysis of water suspensions |
CN2760546Y (en) * | 2004-12-25 | 2006-02-22 | 中国科学院安徽光学精密机械研究所 | Water body chlorophyll thickness measuring apparatus using novel exciting light source |
CN101034055A (en) * | 2007-03-23 | 2007-09-12 | 中国兵器工业集团第五三研究所 | N2O4 corresponding water content standard substance and using method |
CN101858857A (en) * | 2010-05-14 | 2010-10-13 | 同济大学 | Analyzing and measuring method for chlorophyll a of phytoplankton in eutrophic water |
CN102023136A (en) * | 2010-05-21 | 2011-04-20 | 湖北紫鑫生物科技有限公司 | Method for detecting purple sweet potato anthocyanidin pigment by ultraviolet spectrophotometry |
CN102103084A (en) * | 2009-12-18 | 2011-06-22 | 烟台海岸带可持续发展研究所 | Instrument and method for classifying and discriminating algae based on chlorophyll analysis |
CN102638308A (en) * | 2012-02-22 | 2012-08-15 | 大连大学 | Method for analyzing underwater light transmission characteristic |
CN102749313A (en) * | 2012-07-05 | 2012-10-24 | 同济大学 | Remote measuring system for vertical distribution concentration of chlorophyll in seawater |
CN103115879A (en) * | 2013-01-18 | 2013-05-22 | 北京林业大学 | Method for measuring content of chlorophyll a, chlorophyll b and carotenoid in plant live leaf by utilizing absorption spectrum |
CN104089910A (en) * | 2014-06-17 | 2014-10-08 | 广西壮族自治区机械工业研究院 | Apparatus for simultaneously detecting color value and turbidity of finished sugar, and rapid detection method |
CN104777108A (en) * | 2015-02-04 | 2015-07-15 | 中国农业大学 | Detection apparatus and method for content of chlorophyll |
CN105277494A (en) * | 2015-10-15 | 2016-01-27 | 南昌航空大学 | Spectrum detection method for industrial alcohol and edible alcohol |
CN205404410U (en) * | 2016-02-20 | 2016-07-27 | 中国科学院烟台海岸带研究所 | Double -light -path method littoral zone water chlorophyll normal position monitoring devices |
CN107192680A (en) * | 2017-04-18 | 2017-09-22 | 山东省科学院海洋仪器仪表研究所 | A kind of marine biofouling at initial stage monitoring and evaluation method |
-
2018
- 2018-11-28 CN CN201811430223.6A patent/CN109238993A/en active Pending
Patent Citations (14)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0915338A2 (en) * | 1997-11-10 | 1999-05-12 | Jeacle Limited | Photometric analysis of water suspensions |
CN2760546Y (en) * | 2004-12-25 | 2006-02-22 | 中国科学院安徽光学精密机械研究所 | Water body chlorophyll thickness measuring apparatus using novel exciting light source |
CN101034055A (en) * | 2007-03-23 | 2007-09-12 | 中国兵器工业集团第五三研究所 | N2O4 corresponding water content standard substance and using method |
CN102103084A (en) * | 2009-12-18 | 2011-06-22 | 烟台海岸带可持续发展研究所 | Instrument and method for classifying and discriminating algae based on chlorophyll analysis |
CN101858857A (en) * | 2010-05-14 | 2010-10-13 | 同济大学 | Analyzing and measuring method for chlorophyll a of phytoplankton in eutrophic water |
CN102023136A (en) * | 2010-05-21 | 2011-04-20 | 湖北紫鑫生物科技有限公司 | Method for detecting purple sweet potato anthocyanidin pigment by ultraviolet spectrophotometry |
CN102638308A (en) * | 2012-02-22 | 2012-08-15 | 大连大学 | Method for analyzing underwater light transmission characteristic |
CN102749313A (en) * | 2012-07-05 | 2012-10-24 | 同济大学 | Remote measuring system for vertical distribution concentration of chlorophyll in seawater |
CN103115879A (en) * | 2013-01-18 | 2013-05-22 | 北京林业大学 | Method for measuring content of chlorophyll a, chlorophyll b and carotenoid in plant live leaf by utilizing absorption spectrum |
CN104089910A (en) * | 2014-06-17 | 2014-10-08 | 广西壮族自治区机械工业研究院 | Apparatus for simultaneously detecting color value and turbidity of finished sugar, and rapid detection method |
CN104777108A (en) * | 2015-02-04 | 2015-07-15 | 中国农业大学 | Detection apparatus and method for content of chlorophyll |
CN105277494A (en) * | 2015-10-15 | 2016-01-27 | 南昌航空大学 | Spectrum detection method for industrial alcohol and edible alcohol |
CN205404410U (en) * | 2016-02-20 | 2016-07-27 | 中国科学院烟台海岸带研究所 | Double -light -path method littoral zone water chlorophyll normal position monitoring devices |
CN107192680A (en) * | 2017-04-18 | 2017-09-22 | 山东省科学院海洋仪器仪表研究所 | A kind of marine biofouling at initial stage monitoring and evaluation method |
Non-Patent Citations (4)
Title |
---|
《高技术要览》编委会: "《高技术要览 激光卷》", 31 October 2003, 中国科学技术出版社 * |
史毓阶: "《汉英医学装备科学仪器分类词典》", 31 March 2000, 中国医药科技出版社 * |
崔建升: "分光光度法测定水体中叶绿素a的浓缩方法的改进", 《CA理化检验-化学分册》 * |
贾晓平: "《广东海洋渔业资源调查评估与可持续利用对策》", 30 June 2005, 海军出版社 * |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN113746544A (en) * | 2021-09-09 | 2021-12-03 | 桂林电子科技大学 | Vertical channel link layering method for non-uniform seawater |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN107101943B (en) | Device and method for detecting microalgae activity by optical flow control lens-free holographic imaging | |
Zeng et al. | Development of in situ sensors for chlorophyll concentration measurement | |
CN102967604A (en) | Reflectance spectrum measuring and sampling system and method used for jewel detection | |
CN107688635B (en) | Based on the rear Alga Identify Method to polarization scattering and the device applied to this method | |
CN103149158B (en) | A kind of biprism water quality monitoring optical fiber sensing system | |
CN110907375B (en) | Full-spectrum water quality online monitoring device and method | |
CN108846203A (en) | The method and device of fruit non-destructive testing | |
CN101275912A (en) | Fluid food browning testing method | |
CN102175638A (en) | Device for rapidly and nondestructively detecting component content of yellow rice wine | |
CN115046943B (en) | Water quality detection method, device and system and storage medium | |
Konovalov et al. | Determination of the concentration of mineral particles and suspended organic substance based on their spectral absorption | |
SE540009C2 (en) | Method and apparatus for determining a concentration of a substance in a liquid medium | |
CN109238993A (en) | The detection method that Determination of Chlorophyll In Seawater content influences underwater optical transmission characteristics | |
Ahmed et al. | Separation of fluorescence and elastic scattering from algae in seawater using polarization discrimination | |
CN208076382U (en) | Water body multi-wavelength optical attenuation coefficient measuring device | |
CN110907388A (en) | Oil spill type identification method based on infrared spectroscopic analysis | |
CN106932373A (en) | Total organic carbon optics home position sensing | |
CN108279225A (en) | Phytoplankton & Suspension red tide and its toxicity detection optics home position sensing | |
GB2604677A (en) | A method and system for online detection and identification of ichthyotoxic harmful algal bloom algae | |
CN108414464A (en) | Water body multi-wavelength optical attenuation coefficient measuring device and method | |
NL2034211B1 (en) | Method and system for quantitatively identifying multi-pollution sources of mixed water body | |
CN202002879U (en) | Yellow wine ingredient content detecting device | |
CN101625306B (en) | Device for measuring gas concentration | |
CN110887814A (en) | Underwater turbidity detection method based on spectral analysis | |
Arst et al. | Calculating irradiance penetration into water bodies from the measured beam attenuation coefficient |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20190118 |
|
WD01 | Invention patent application deemed withdrawn after publication |