CN104774807A - 将脐带间充质干细胞诱导分化成少突胶质细胞的方法 - Google Patents
将脐带间充质干细胞诱导分化成少突胶质细胞的方法 Download PDFInfo
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Abstract
Description
序号 | 诱导方式 | 分化比例 |
1(对照) | 不加入诱导物 | 12.02% |
2(对照) | 加入***-1(IGF-1) | 22.14% |
3(根据本发明) | 加入式I所示化合物 | 32.56% |
4(根据本发明) | 加入式II所示化合物 | 35.12% |
5(根据本发明) | 加入式III所示化合物 | 37.78% |
6(根据本发明) | 加入式I、II和III所示化合物1:2:2重量比的混合物 | 43.69% |
Claims (6)
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108624560A (zh) * | 2018-06-01 | 2018-10-09 | 南京艾尔普再生医学科技有限公司 | 一种分化培养基及少突胶质前体细胞的制备方法 |
CN114763532A (zh) * | 2021-01-12 | 2022-07-19 | 内蒙古大学 | 神经干细胞分化为少突胶质细胞的方法、培养基及用途 |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101326281A (zh) * | 2005-10-13 | 2008-12-17 | 人类起源公司 | 用源自胎盘的干细胞制备少突胶质细胞 |
CN102337246A (zh) * | 2011-10-12 | 2012-02-01 | 北京弘润天源生物技术有限公司 | 刺五加苷分化间充质干细胞成少突胶质前体细胞及试剂盒的研制、用于神经***疾病的药物 |
CN102559593A (zh) * | 2011-11-22 | 2012-07-11 | 杭州师范大学 | 一种从人胚胎干细胞分化成神经细胞的方法 |
CN103045531A (zh) * | 2011-10-14 | 2013-04-17 | 北京清美联创干细胞科技有限公司 | 人参皂苷Rd诱导间充质干细胞体外定向分化成肝细胞 |
CN104232573A (zh) * | 2014-09-11 | 2014-12-24 | 安沂华 | 一种生长培养基及其用途和培养脐带间充质干细胞的方法 |
-
2015
- 2015-04-28 CN CN201510207336.XA patent/CN104774807B/zh not_active Expired - Fee Related
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101326281A (zh) * | 2005-10-13 | 2008-12-17 | 人类起源公司 | 用源自胎盘的干细胞制备少突胶质细胞 |
CN102337246A (zh) * | 2011-10-12 | 2012-02-01 | 北京弘润天源生物技术有限公司 | 刺五加苷分化间充质干细胞成少突胶质前体细胞及试剂盒的研制、用于神经***疾病的药物 |
CN103045531A (zh) * | 2011-10-14 | 2013-04-17 | 北京清美联创干细胞科技有限公司 | 人参皂苷Rd诱导间充质干细胞体外定向分化成肝细胞 |
CN102559593A (zh) * | 2011-11-22 | 2012-07-11 | 杭州师范大学 | 一种从人胚胎干细胞分化成神经细胞的方法 |
CN104232573A (zh) * | 2014-09-11 | 2014-12-24 | 安沂华 | 一种生长培养基及其用途和培养脐带间充质干细胞的方法 |
Non-Patent Citations (1)
Title |
---|
庄朋伟 等: "人参皂苷Rg1 促进体外培养神经干细胞增殖的研究", 《中国中药杂志》 * |
Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108624560A (zh) * | 2018-06-01 | 2018-10-09 | 南京艾尔普再生医学科技有限公司 | 一种分化培养基及少突胶质前体细胞的制备方法 |
EP3805374A4 (en) * | 2018-06-01 | 2022-03-23 | Help Stem Cell Innovations Co., Ltd. | DIFFERENTIATION MEDIUM AND OLIGODENDROCYTE PRECURSOR PREPARATION METHOD |
CN108624560B (zh) * | 2018-06-01 | 2022-04-08 | 南京艾尔普再生医学科技有限公司 | 一种分化培养基及少突胶质前体细胞的制备方法 |
CN114763532A (zh) * | 2021-01-12 | 2022-07-19 | 内蒙古大学 | 神经干细胞分化为少突胶质细胞的方法、培养基及用途 |
CN114763532B (zh) * | 2021-01-12 | 2023-12-12 | 内蒙古大学 | 神经干细胞分化为少突胶质细胞的方法、培养基及用途 |
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