CN104738236A - Application of Fuzhuan tea in regulation of animal intestinal flora - Google Patents
Application of Fuzhuan tea in regulation of animal intestinal flora Download PDFInfo
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- CN104738236A CN104738236A CN201310754504.8A CN201310754504A CN104738236A CN 104738236 A CN104738236 A CN 104738236A CN 201310754504 A CN201310754504 A CN 201310754504A CN 104738236 A CN104738236 A CN 104738236A
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Abstract
The invention discloses application of Fuzhuan tea in regulation of animal intestinal flora. The Fuzhuan tea can be used for obviously increasing the number of such probiotics in animal intestines as lactobacillus acetotoleran, butyrivibrio, Luosibairui Escherichia, Roseburia Stanton and Savage and the like and significantly regulating the animal intestinal flora, so as to achieve an effect of reducing blood fat through the functions of the intestinal flora.
Description
Technical field
The present invention relates to the novelty teabag of Fu tea, particularly, relate to Fu tea and regulating the purposes in microbial population of animal intestinal tract.
Background technology
Perch the normal flora of about 104 orders of magnitude in human gastrointestinal tract, its total amount and liver similar.Enteric microorganism contains miscellaneous enzyme system, can participate in a series of physiology courses such as host's energy, material and hereditary information transhipment.Existing many research proves, enteric microorganism can form biological barrier at enteron aisle, by occupation time process, nutrient competition and the undue growth of rejection condition pathogenic bacteria and the invasion of external pathogenic bacteria such as secreted various metabolite and bacteriocin.Increasing evidence shows, the gut flora of structural imbalance has a very important role in the generation, development of obesity, high fat of blood chronic diseases.
But, regulate the research of microbial population of animal intestinal tract aspect still to need deeply at present.
Summary of the invention
The present invention is intended at least to solve one of technical problem existed in prior art.For this reason, one object of the present invention is to propose a kind of means that effectively can regulate microbial population of animal intestinal tract.
In one aspect of the invention, the present invention proposes Fu tea and regulate the purposes in microbial population of animal intestinal tract.According to embodiments of the invention, Fu tea significantly can regulate the gut flora of animal, and then is played effect of reducing blood lipid by the effect of gut flora.Thus, Fu tea is provided can effectively to regulate its gut flora to animal, thus prevention or treatment hyperlipidaemic conditions.
It should be noted that, the animal species of term " animal " indication in expression way " Fu tea is regulating the purposes in microbial population of animal intestinal tract " used in this article is not particularly limited.According to embodiments of the invention, this animal can be any animal with enteron aisle organ, and preferred mammal, more preferably rat, mouse, people, optimum is chosen.In addition, used in this article expression way " prevention or treatment " mainly refers to prevention or treatment high fat of blood.
Term during expression way used herein " provides Fu tea to animal " " provides " and refers to that described animal drinks Fu tea, and the mode of drinking can be that animal is initiatively drunk, and also can be artificial gavage.
Fu tea can be used in regulating microbial population of animal intestinal tract, and then can be played effect of reducing blood lipid by the effect of gut flora.Thus, according to embodiments of the invention, described Fu tea is used for prevention or treatment high fat of blood.
According to embodiments of the invention, drink Fu tea without any side effect, thus the dosage of drinking of Fu tea is not particularly limited, as long as can improve the quantity of the probios such as genus lactubacillus in animal subject enteron aisle, Butyrivibrio, Luo Sibairui Bordetella, Luo Si Bordetella and effectively regulate the gut flora of animal.According to concrete examples more of the present invention, described Fu tea is supplied to described animal with the dosage of 150mg/kg, wherein, the fall apart viable count of capsule bacterium of coronoid process is not less than 4 × 10
4cFU.Thus, in animal subject enteron aisle, the quantity of probio increases obviously, and namely Fu tea regulates the Be very effective of microbial population of animal intestinal tract.
Also it should be noted that, Fu tea of the present invention is regulating the purposes in microbial population of animal intestinal tract, and the present inventor just surprisingly finds through arduous creative work and a large amount of experimental works just.
Beneficial effect of the present invention is:
1, Fu tea of the present invention, all raw materials are taken at nature, it is natural to come from, and not containing any chemicals, have no side effect, safe and reliable; Raw material sources are extensively easy to get, and preparation technology is simple, and storing and application safety, low production cost, is easily esthetically acceptable to the consumers.
2, Fu tea of the present invention all proves without any side effects through animal function test and test drunk by people, has significant reducing blood lipid, regulates the effects such as microbial population of animal intestinal tract.The reduction blood fat of Fu tea energy highly significant of the present invention, can make patient's fasting plasma lipid significantly decline.This Fu tea is suitable for high fat of blood, obese people and elderly population etc., is drunk by daily, from the angle of effecting a permanent cure, by improving lipid-metabolism, improving blood flow, improving body immunity etc., finally reaches the object protected the health.
3, present invention achieves easily, the health-care efficacy such as significant reducing blood lipid, successful, security are good, and steady quality, product can be preserved for a long time, for people's daily health caring provides new selection.
Additional aspect of the present invention and advantage will part provide in the following description, and part will become obvious from the following description, or be recognized by practice of the present invention.
Detailed description of the invention
Embodiments of the invention are described below in detail.Embodiment described below is exemplary, only for explaining the present invention, and can not be interpreted as limitation of the present invention.Unreceipted concrete technology or condition in embodiment, (such as show with reference to J. Pehanorm Brooker etc. according to the technology described by the document in this area or condition, " Molecular Cloning: A Laboratory guide " that Huang Peitang etc. translate, the third edition, Science Press) or carry out according to product description.Agents useful for same or the unreceipted production firm person of instrument, be and by the conventional products of commercial acquisition, such as, can be able to purchase from Illumina company.
In one aspect of the invention, the present invention proposes Fu tea and regulate the purposes in microbial population of animal intestinal tract.According to embodiments of the invention, Fu tea significantly can regulate the gut flora of animal, and then is played effect of reducing blood lipid by the effect of gut flora.Thus, Fu tea is provided can effectively to regulate its gut flora to animal, thus prevention or treatment hyperlipidaemic conditions.
It should be noted that, the animal species of term " animal " indication in expression way " Fu tea is regulating the purposes in microbial population of animal intestinal tract " used in this article is not particularly limited.According to embodiments of the invention, this animal can be any animal with enteron aisle organ, and preferred mammal, more preferably rat, mouse, people, optimum is chosen.In addition, used in this article expression way " prevention or treatment " mainly refers to prevention or treatment high fat of blood.
Term during expression way used herein " provides Fu tea to animal " " provides " and refers to that described animal drinks Fu tea, and the mode of drinking can be that animal is initiatively drunk, and also can be artificial gavage.
Fu tea can be used in regulating microbial population of animal intestinal tract, and then can be played effect of reducing blood lipid by the effect of gut flora.Thus, according to embodiments of the invention, described Fu tea is used for prevention or treatment high fat of blood.
According to embodiments of the invention, drink Fu tea without any side effect, thus the dosage of drinking of Fu tea is not particularly limited, as long as can improve the quantity of the probios such as genus lactubacillus in animal subject enteron aisle, Butyrivibrio, Luo Sibairui Bordetella, Luo Si Bordetella and effectively regulate the gut flora of animal.According to concrete examples more of the present invention, described Fu tea is supplied to described animal with the dosage of 150mg/kg, wherein, the fall apart viable count of capsule bacterium of coronoid process is not less than 4 × 10
4cFU.Thus, in animal subject enteron aisle, the quantity of probio increases obviously, and namely Fu tea regulates the Be very effective of microbial population of animal intestinal tract.
For ease of understanding, be described Fu tea of the present invention and preparation method thereof can be applied to below:
In another aspect of the present invention, the present invention proposes a kind of method preparing Fu tea.According to embodiments of the invention, the method comprises step:
Prepare eurotium cristatum spore suspension:
(1) the yellow cleistothecium on Fu-brick tea block is inoculated on Czapek's medium, cultivates 72h in 28 DEG C; (2) be separated cultivating the yellow cleistothecium plate streaking obtained in step (1) 2 ~ 3 times, to obtain the bacterium colony of preliminary purification; (3) bacterium colony of described preliminary purification is placed in sterilized water, uniform spore suspension is made in jolting, and carries out 10 times and increase progressively dilution, makes the dilution spore suspension of 10-3 ~ 10-5; (4) spore suspension obtained in step (3) is carried out flat board coating on blank Cha Shi culture medium, cultivate at being then placed in 28 DEG C, to obtain pure culture bacterium colony; (5) prepare the dilution spore suspension of 10-1 ~ 10-3 according to step (3), and spore concentration is adjusted to 1.5 × 10
6~ 2.0 × 10
6individual/ml; (6) spore suspension obtained in step (5) is inoculated in liquid CZG culture medium, in 28 DEG C, under 150r/min condition, cultivates 96h, to obtain described eurotium cristatum spore suspension.Thereby, it is possible to effectively prepare eurotium cristatum spore suspension.
According to embodiments of the invention, the concentration of described eurotium cristatum spore suspension is not particularly limited, and those skilled in the art can select as the case may be flexibly.According to one embodiment of present invention, the concentration of described eurotium cristatum spore suspension is 1.0 × 10
7-1 × 10
8individual/ml.Thus, be conducive to Hou Xu ?the fermentation of gross tea.
Inoculation fermentation:
Described eurotium cristatum spore suspension is connect and plants in ?gross tea, and ferment, to obtain Fu tea.Thereby, it is possible to effectively prepare the luxuriant Fu tea of golden flower (namely coronoid process falls apart capsule bacterium, and " golden flower " and " coronoid process fall apart capsule bacterium " can exchange use in this article), and method simple, hold easy operation control.
According to embodiments of the invention, described fermentation is at 28 degrees Celsius, carries out under the condition of humidity 70%.Thereby, it is possible to Shi get ?gross tea ferment under optimal temperature, damp condition, be beneficial to coronoid process and fall apart the growth of capsule bacterium.
According to embodiments of the invention, the time of described fermentation is 7 ~ 12 days.You Ci ， ?gross tea can effectively ferment.
In another aspect of the invention, the present invention proposes a kind of Fu tea.According to embodiments of the invention, described Fu tea is prepared by foregoing method.Inventor is surprised to find, and provides Fu tea of the present invention to animal, effectively can regulate the gut flora of animal, and then based on the effect of gut flora, plays effect of reducing blood lipid.
In still another aspect of the invention, the invention allows for a kind of method regulating microbial population of animal intestinal tract.According to embodiments of the invention, the method provides foregoing Fu tea to described animal.According to embodiments of the invention, utilize method of the present invention can significantly improve the quantity of the probios such as genus lactubacillus in animal subject enteron aisle, Butyrivibrio, Luo Sibairui Bordetella, Luo Si Bordetella, the gut flora of effective adjustment animal, and then effectively prevent by the effect of gut flora or treat hyperlipidaemic conditions.
The preparation of embodiment 1: Fu tea
1, the preparation of eurotium cristatum spore suspension
Be inoculated on Czapek's medium with the yellow cleistothecium on aseptic inoculation pin picking Fu-brick tea block, cultivate in 28 DEG C.In time cultivating 72h, a small amount of yellow cleistothecium of picking makes plate streaking and is separated, and after 2 ~ 3 plate streakings, this bacterium obtains preliminary purification.When the colony growth of preliminary purification is in animated period, scrape extracting yellow cleistothecium with oese and put into the sterile vials filling 10ml sterilized water, jolting 20min, make uniform spore suspension and carry out 10 times and increase progressively dilution, make 10
-3~ 10
-5dilution spore suspension.
Draw each dilution spore suspension of 0.1ml respectively with aseptic straw on blank Cha Shi culture medium, carry out flat board coating, cultivate at being then placed in 28 DEG C.Choose the flat board that only length has 1 ~ 2 single bacterium colony, the single bacterium colony on this flat board is exactly the pure culture of this bacterium, observes its colony characteristics, and in conjunction with microscopic examination individual morphology feature.
Prepare 10 according to the method described above
-1~ 10
-3dilution spore suspension, then carries out blood counting chamber counting to spore suspension, spore concentration is adjusted to 1.5 × 10
6~ 2.0 × 10
6individual/ml, with pipettor draw 1ml spore suspension be inoculated in prepared be equipped with in the 250ml triangular flask of 99ml liquid CZG culture medium, in 28 DEG C, cultivation 96h is carried out under 150r/min condition, bacterium liquid is splined on blood counting chamber and carries out spore count under an optical microscope, the concentration of adjustment spore is 1.0 × 10
7-1 × 10
8individual/ml, obtains eurotium cristatum spore suspension thus.
Czapek's medium: sodium nitrate 2g/L, dipotassium hydrogen phosphate 1g/L, potassium chloride 0.5g/L, magnesium sulfate 0.5g/L, ferric sulfate 0.01g/L, sucrose 30g/L, agar 15 ~ 20g/L.
CZG culture medium: sucrose 40g/L, dipotassium hydrogen phosphate 1g/L, nitric acid ammonia 3g/L, sodium chloride 50g/L, magnesium sulfate 0.5g/L, agar 15 ~ 20g/L.
2, Dark Green Tea inoculation fermentation
Accurately take 200g Dark Green Tea, inoculation 10ml eurotium cristatum spore suspension, in 28 DEG C, under the condition of humidity 70%, fermentation 7-12 days, then samples according to the regulation of GB8302-87 " tea sampling ", and counting, with Fu-brick tea national standard for criterion of acceptability.
3, pack
The Dark Green Tea fermented is weighed, is pressed into brick tea or directly packs.
Embodiment 2: Fu tea mediator gut flora functional experiment
Regulating intestinal canal flora function human body according to the Ministry of Public Health " health beverages inspection and assessment technical specification (2003) " is tried out experimental evaluation way and is evaluated the Fu tea that embodiment 1 prepares, specific as follows:
1. try out experiment grouping
Experiment grouping sheet tried out by table 1
2. human body tries out experimental technique
Experimenter is divided at random 2 groups, often organizes 20 people, before experimenter tries out, all asepticly take experimenter's ight soil, 16S rDNA checks order inspection gut flora, as the reference of background level.
By the grouping situation of table 1, group 1 takes control group, group 2 takes experimental group, after 4 weeks, takes experimenter's ight soil, and 16SrDNA order-checking inspection gut flora, contrasts with background level, contrast the multiple that its relative abundance increases.
3. gut flora detection method
First fecal sample carries out DNA extraction, V3 ~ V5 region of pcr amplification 16S rDNA, then with 454 platform order-checkings, and order-checking direction V5->V3.Average 7795 tag of each sample initial data, read long about 400bp.The final tag number average out to 3235 for analyzing, reads long about 265bp.
16S analyzes main employing mothur software (http://www.mothur.org/wiki/Mothur_manual), comprises Quality Control, OTU cluster, the analyses such as annotation, and on the basis completing data species taxonomy, analyzes the relative abundance of each species.
4. experimental result
Concrete outcome is in table 2:
The sequencing data abundance contrast of each Pseudomonas of table 2 people gut flora (with try out compared with front background level improve multiple)
As shown in Table 2, taken the experimental group of Fu tea relative to control group, the probio number such as genus lactubacillus, Butyrivibrio, Luo Sibairui Bordetella, Luo Si Bordetella all has obvious increase, shows that Fu tea regulates the Be very effective of microbial population of animal intestinal tract.
Embodiment 3: Fu tea reducing blood lipid human experiment is tested
Auxiliary lipid-lowering function human experiment experimental evaluation way according to the Ministry of Public Health " health food inspection and assessment technical specification (2003) " is evaluated the Fu tea that embodiment 1 prepares, specific as follows:
1, human experiment and test method:
Select 20 example II type people with hyperlipidemia by the principle of voluntariness, the wherein male sex 16 people, women 4 people, the range of age 43-75 year, without complication such as serious conscience kidneys, contrast design after taking in before test-meal.
Experimenter is divided into 2 groups at random, often organizes 10 people, experimenter's former medicine kind, diet control and activity are constant, and experimental group takes Fu tea of the present invention, and control group takes physiological saline, and taking dose is with reference to table 1.
The change of monitoring experimenter blood pressure, stool and urine, body weight, measures blood fat (T-CHOL TC, triglycerides TG and HDL HDL-C).
2, human experiment result:
About the impact on the blood fat before and after experimenter's test-meal of taking Fu tea of the present invention, compared with before test-meal, the cholesterol of test-meal experimenter after 30 days, triglyceride comparatively all have obvious reduction before test-meal, and HDL comparatively has obvious rising before test-meal, and concrete outcome is in table 3.
Crowd's lipids detection result tried out by table 3
Note: (1) * represents P<0.05; (2) * * represents P<0.01
As shown in Table 3, Fu tea of the present invention has the health care of obvious reducing blood lipid, and to the harmless effect of tested population health.
In the description of this description, specific features, structure, material or feature that the description of reference term " embodiment ", " some embodiments ", " example ", " concrete example " or " some examples " etc. means to describe in conjunction with this embodiment or example are contained at least one embodiment of the present invention or example.In this manual, identical embodiment or example are not necessarily referred to the schematic representation of above-mentioned term.And the specific features of description, structure, material or feature can combine in an appropriate manner in any one or more embodiment or example.
Although illustrate and describe embodiments of the invention, those having ordinary skill in the art will appreciate that: can carry out multiple change, amendment, replacement and modification to these embodiments when not departing from principle of the present invention and aim, scope of the present invention is by claim and equivalents thereof.
Claims (3)
1. Fu tea is regulating the purposes in microbial population of animal intestinal tract.
2. purposes according to claim 1, is characterized in that, described Fu tea is used for prevention or treatment high fat of blood.
3. purposes according to claim 2, is characterized in that, by described Fu tea to be not less than 4 × 10
4the fall apart dosage of capsule bacterium/(kgd) of CFU coronoid process is supplied to described animal.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106011047A (en) * | 2016-05-16 | 2016-10-12 | 陕西文冠果实业集团有限公司 | Preparation method for eurotium cristatum suspension liquid for shinyleaf yellowhorn Fuzhuang tea |
| CN107753527A (en) * | 2016-08-17 | 2018-03-06 | 宋福行 | Fu tea extractions and its application in regulation blood fat and liver fat |
| CN109223834A (en) * | 2018-11-14 | 2019-01-18 | 长沙天赐生物医药科技有限公司 | A kind of bifidobacterium and its application |
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| CN101249112A (en) * | 2008-03-21 | 2008-08-27 | 贺志弘 | Fat-reducing products containing black tea Eurotium Cristatum and preparation thereof |
| CN103300177A (en) * | 2013-06-13 | 2013-09-18 | 彭呈悦 | Method and application for increasing oligomerization wood sugar content in black tea |
| CN103305565A (en) * | 2013-06-13 | 2013-09-18 | 彭呈悦 | Preparation method of eurotium cristatum mycelium extract |
| CN103405757A (en) * | 2013-08-28 | 2013-11-27 | 刘基林 | Composition for health-care product, health-care product and using method thereof |
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2013
- 2013-12-31 CN CN201310754504.8A patent/CN104738236A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101249112A (en) * | 2008-03-21 | 2008-08-27 | 贺志弘 | Fat-reducing products containing black tea Eurotium Cristatum and preparation thereof |
| CN103300177A (en) * | 2013-06-13 | 2013-09-18 | 彭呈悦 | Method and application for increasing oligomerization wood sugar content in black tea |
| CN103305565A (en) * | 2013-06-13 | 2013-09-18 | 彭呈悦 | Preparation method of eurotium cristatum mycelium extract |
| CN103405757A (en) * | 2013-08-28 | 2013-11-27 | 刘基林 | Composition for health-care product, health-care product and using method thereof |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106011047A (en) * | 2016-05-16 | 2016-10-12 | 陕西文冠果实业集团有限公司 | Preparation method for eurotium cristatum suspension liquid for shinyleaf yellowhorn Fuzhuang tea |
| CN107753527A (en) * | 2016-08-17 | 2018-03-06 | 宋福行 | Fu tea extractions and its application in regulation blood fat and liver fat |
| CN109223834A (en) * | 2018-11-14 | 2019-01-18 | 长沙天赐生物医药科技有限公司 | A kind of bifidobacterium and its application |
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