CN104719635A - Method for preparing feed additive mixed and fermented by aquatic product leftover and Chinese herbal medicine - Google Patents
Method for preparing feed additive mixed and fermented by aquatic product leftover and Chinese herbal medicine Download PDFInfo
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Abstract
The invention discloses a method for preparing feed additive mixed and fermented by an aquatic product leftover and a Chinese herbal medicine. The method comprises the following steps: mincing the fresh aquatic product leftover for later use, smashing the Chinese herbal medicine and enabling the smashed Chinese herbal medicine to pass through a 60-mesh sieve for later use; mixing the smashed aquatic product leftover and the smashed Chinese herbal medicine, adding a nutriment and water, and mixing uniformly as a fermentation medium; sterilizing and cooling the fermentation medium, inoculating bacillus subtilis HL-1 for producing antimicrobial lipopeptide, performing solid aerobic fermentation, drying and smashing and packaging the fermented product to obtain the feed additive product. The feed additive prepared by the method is rich in antimicrobial lipopeptide, the Chinese herbal medicine effective constituents and a nutrient small peptide, has good growth promotion and disease-resistant performance, can be used for completely substituting the feed antibiotic in the feed industry and has good application prospect.
Description
Technical field
The present invention relates to the preparation method of the feed addictive of a kind of aquatic product leftovers and Chinese herbal medicine mixed culture fermentation, belong to technical field prepared by feed addictive.
Background technology
There are 9.7 ten thousand tons for livestock breeding industry in about 210,000 tons of antibiotic raw materials that current China produces every year, account for gross annual output amount 46.1%.Excessive use antibiotic not only result in the drug resistance of cultivated animals to antibiotic medicine, the resistance against diseases of cultivated animals is declined, and these antibiotic can enter human body with food chain, human body is made inadvertently to produce drug-resistant bacteria, treatment difficulty and expense will be increased once sick, even likely can produce " superbacteria ", occur situation about pasting medical help.Along with improving constantly of people's quality of life, safe and sanitary varieties of food items being comprised to meat product has had higher requirement, also day by day strong to the cry of " quality-assured meat ", " pollution-free food ".Therefore, finding antibiotic green substitute is China's feed and problem demanding prompt solution in cultivation industry development.
Chinese herbal medicine is the rarity in Chinese medicine treasure-house, and Chinese herbal medicine is as feed addictive, starts in Ancient Times in China.Chinese herbal medicine has and has no side effect, not easily causes the characteristic such as resistance, noresidue, and the green substitute as a kind of feeding antibiotic has been widely recognized at feed industry.From the eighties in 20th century, Chinese herbal medicine has been used to make feeding antibiotic substitute, and achieves certain effect.After Chinese herbal medicine undergoes microbial fermentation, its active ingredient is fully discharged, and makes it have more biologically active and curative effect study hotspot especially in recent years.China's feed (2010,419(15): 13-17) document discloses and from different herbal raw material, is separated the aspergillus oryzae obtained ferments to Chinese herbal medicine, and the bacteriostatic activity of fermentation material cooking liquor to Escherichia coli, staphylococcus aureus, proteus vulgaris and salmonella typhimurium comparatively contrasts raising 36.03%, 60.83%, 27.64%, 41.32% respectively.But be just confined to fully allow Chinese herbal medicine effective ingredients discharge to the fermentation of Chinese herbal medicine above, thus improve bacteriostatic activity, but the antibacterial activity of Chinese herbal medicine effective ingredients does not promote.It is that using dosage is bigger than normal that current fermentation of Chinese herbal medicine exists maximum problem.This not only adds the use cost of product, but also affect feed nutrition proportioning and palatability, thus fermentation of Chinese herbal medicine is not still widely used at feed industry.Therefore, be badly in need of the antibacterial activity increasing substantially active ingredient in fermentation of Chinese herbal medicine, reduce the using dosage of fermentation of Chinese herbal medicine at feed industry, to promote that fermentation of Chinese herbal medicine is in the extensive use of feed industry.
The green substitute of one as feeding antibiotic, antibacterial peptide usually has heat-resisting, has a broad antifungal spectrum, good water solubility and not easily produces the characteristics such as drug resistance, receives much concern in recent years.Antibacterial lipopeptid be with bacillus subtilis (
bacillus subtilis), bacillus amyloliquefaciens (
bacillus amyloliquefaciens), Bacillus natto (
bacillus natto) etc. a series of fat peptide matters of producing for the bacillus of representative.Antibacterial lipopeptid studies have reported that in the application of feed industry in recent years.Patent CN 101974586 A disclose a kind of bacillus subtilis (
bacillus subtilis) production method of antibacterial lipopeptid and the application in feed.Bacillus subtilis (
bacillus subtilis) after liquid fermentation, concentrated, spraying dry, make a kind of tiring be the antibacterial lipopeptid product of 20000 ~ 300000IU/g to fmbj.In daily ration of broiler by 4000 IU/ kilograms add antibacterial lipopeptids, improve efficiency of feed utilization, and to broiler chicken grow and the enhancing of immunity all has good effect.Although antibacterial lipopeptid has a good application prospect at feed industry, at present because production cost is high, do not obtain practical application at feed industry.Thus, reducing the production cost of antibacterial lipopeptid or the using dosage of antibacterial lipopeptid, thus reduced use cost, is promote that antibacterial lipopeptid applies the required technical problem solved widely at feed industry.
Summary of the invention
The present invention for raw material, prepares a kind of compound feeding antibiotic substitute that be rich in Chinese herbal medicine effective ingredients and antibacterial lipopeptid by the method for solid state fermentation with aquatic product leftovers and Chinese herbal medicine.Use cost is there is high for existing Chinese herbal medicine fermented product and these two kinds of feeding antibiotic substitutes of antibacterial lipopeptid, do not applied on a large scale at feed industry, the object of the invention is not only will to reduce the production cost that antibacterial lipopeptid and Chinese herbal medicine ferment, but also the antibacterial activity of Chinese herbal medicine effective ingredients and antibacterial lipopeptid will be improved, thus reduce antibacterial lipopeptid and the Chinese herbal medicine using dosage at feed industry, to promote that antibacterial lipopeptid and Chinese herbal medicine are in the application widely of feed industry.
In order to solve the problem, the technical solution adopted in the present invention is as follows: the preparation method of the feed addictive of a kind of aquatic product leftovers and Chinese herbal medicine mixed culture fermentation, comprises the steps:
(1) by slant preservation can produce antibacterial lipopeptid bacillus subtilis (
bacillus subtilis) in the LB fluid nutrient medium that cools after being linked into sterilizing of HL-1, in 37 DEG C, 150r/min cultivates 16h, be prepared into bacillus subtilis (
bacillus subtilis) HL-1 seed liquor is for subsequent use;
(2) for subsequent use after fresh aquatic product leftovers being rubbed, cross 60 mesh sieves after being pulverized by Chinese herbal medicine for subsequent use;
(3) aquatic product leftovers of rubbing step (2) obtained and the rear Chinese herbal medicine crossing 60 mesh sieves of pulverizing are that the ratio of 0.5 ~ 1.2:1 mixes in mass ratio, and add nutrients and water mixing, obtain final moisture content and control the fermentation medium in 40% ~ 80% scope;
(4) fermented and cultured of mixing step (3) obtained is based on 121 DEG C of sterilizing 30min;
(5) fermentation medium after sterilizing step (4) obtained is cooled to room temperature, then access bacillus subtilis prepared by step (1) (
bacillus subtilis) HL-1 seed liquor, and mix;
(6) fermentation of raw material: the fermentation medium adding bacterium that step (5) obtains is fermented according to following method: fermentation medium is divided in the fermentation tray of having sterilized, charging thickness is 4cm ~ 8cm, the sterilized wet gauze of fermentation medium surface coverage in tray, again tray is placed in the shelf top fermentation in sterilized solid fermentation room, the temperature that controls environment is at 30 DEG C ~ 32 DEG C, and fermentation time is 40h ~ 50h;
(7) product handling system: fermentation ends, by the product drying obtained after fermentation, after pulverizing and packaging, can obtain the feed addictive finished product of a kind of aquatic product leftovers and Chinese herbal medicine mixed culture fermentation.
In described step (1), the Bacillus subtillis HL-1 of access can produce antibacterial lipopeptid, this bacterium is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on January 20th, 2015, deposit number is CGMCC No.10379, and preservation address is No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City Institute of Microorganism, Academia Sinica.
Aquatic product leftovers in described step (2) is the one in leftovers of tilapia, shrimp head, scallop body, cod leftover bits and pieces, squid leftover bits and pieces, carp leftover bits and pieces, Harengula zunasi leftover bits and pieces, black carp leftover bits and pieces, silver carp leftover bits and pieces, grass carp leftover bits and pieces and stripped tuna leftover bits and pieces; Chinese herbal medicine in described step (2) is a kind of or wherein two or more mixture in the Radix Astragali, honeysuckle, granatum, garden burnet, the coptis, cordate houttuynia, kuh-seng and smilax.
The mixture that nutrients in described step (3) is glucose, potassium chloride, magnesium chloride and dipotassium hydrogen phosphate form; Nutraceutical addition in described step (3) accounts for fermentation medium gross mass percentages with the nutraceutical quality of adding, and is respectively: glucose 0.01% ~ 5%, potassium chloride 0.01% ~ 5%, magnesium chloride 0.01% ~ 5%, dipotassium hydrogen phosphate 0.01% ~ 5%.
The middle bacillus subtilis of described step (5) (
bacillus subtilis) inoculum concentration of HL-1 seed liquor accounts for fermentation medium gross mass percentages with the quality of seed liquor of access, is 0.1% ~ 5%.
The present invention compares with existing technology, has the following advantages.
(1) production cost of the green substitute of feeding antibiotic is reduced.The present invention with produce antibacterial lipopeptid bacillus subtilis (
bacillus subtilis) carry out the method for solid state fermentation, not only can ferment generation antibacterial lipopeptid, but also medium-height grass the effective elements of the medicine is discharged.The fermentation of antibacterial lipopeptid and the fermentation of Chinese herbal medicine unite two into one by the present invention, thus reduce the production cost of the green substitute of feeding antibiotic on the whole.
(2) improve the antibacterial activity of antibacterial lipopeptid and Chinese herbal medicine effective ingredients, thus significantly reduce the using dosage of antibacterial lipopeptid and Chinese herbal medicine effective ingredients.The present invention have selected from thousands of medium-height grass Drug Storage top sieve the Chinese herbal medicine that its active ingredient and antibacterial lipopeptid have collaborative inhibitory effect.The fermentation generation of antibacterial lipopeptid and Chinese herbal medicine are not only fermented and are united two into one by the present invention, and due to the interaction of antibacterial lipopeptid and Chinese herbal medicine effective ingredients, the antibacterial activity of the two all significantly strengthens.This is also the fermentation of single at present Chinese herbal medicine with not available for antibacterial lipopeptid fermenting and producing.Just because of invention increases prepared antibacterial lipopeptid and the antibacterial activity of Chinese herbal medicine effective ingredients, thus the using dosage of the feeding antibiotic substitute prepared by the present invention is greatly reduced, decrease several times to tens times when wherein contained antibacterial lipopeptid is used alone with the actual using dosage ratio of Chinese herbal medicine effective ingredients.
(3) improve growth-promoting effect.In method provided by the invention, aquatic product leftovers is by being degraded into little peptide under the fermentation of microorganism.Little peptide has growth promoting function to letting animals feed, and this has obtained the accreditation of feed industry.Be rich in little peptide, can further improve the growth-promoting effect of green feed additive prepared by the inventive method.
The invention provides the preparation method of the feed addictive of a kind of aquatic product leftovers and Chinese herbal medicine mixed culture fermentation.The present invention not only reduces the production cost of the green substitute of feeding antibiotic; But also greatly reduce the using dosage of lipopeptid and Chinese herbal medicine effective ingredients; And further increase the growth-promoting effect of feeding antibiotic substitute.The culture experiment of the pig of green feed additive in South China of what the present invention obtained be rich in lipopeptid, Chinese herbal medicine effective ingredients and little peptide, chicken, prawn shows, the green feed additive prepared by the inventive method can substitute feeding antibiotic completely, obvious reduction aquaculture cost, obtains significant economic benefit and social benefit.
Detailed description of the invention
In order to understand the present invention better, with regard to the selection of the kind of aquatic product leftovers and Chinese herbal medicine, fermentation parameter and fermentation mode, the invention will be further described in conjunction with the embodiments below, but protection scope of the present invention is not limited thereto.
Embodiment 1: by slant preservation can produce antibacterial lipopeptid bacillus subtilis (
bacillus subtilis) in the LB fluid nutrient medium that cools after being linked into sterilizing of HL-1, in 37 DEG C, 150r/min cultivates 16h, be prepared into bacillus subtilis (
bacillus subtilis) HL-1 seed liquor is for subsequent use; It is for subsequent use after fresh leftovers of tilapia is rubbed; 60 mesh sieves are crossed for subsequent use after being pulverized by the Radix Astragali.By 100 kilograms rub leftovers of tilapia and 100 kilograms pulverize the Radixs Astragali mixing, and add 380 grams of glucose, 400 grams of potassium chloride, 300 grams of magnesium chlorides, 500 grams of dipotassium hydrogen phosphates mix together with 10 kg of water, obtain mixing after fermentation medium; Fermented and cultured after mixing is based on 121 DEG C of sterilizing 30min; Then add in the fermentation medium cooled after sterilizing 1 kilogram of bacillus subtilis (
bacillus subtilis) HL-1 seed liquor, stir and mix; Postvaccinal raw material is divided in the fermentation tray of the alcohol surface sterilization using 75%, charging thickness is 8 centimetres, the sterilized wet gauze of material surface coverage in tray, then tray is placed in the shelf top fermentation in the solid fermentation room of sterilizing, the temperature that controls environment is 30 DEG C, and fermentation time is 50h; After fermentation ends, by the product drying obtained after fermentation, after pulverizing and packaging, namely obtain the feed addictive finished product of a kind of aquatic product leftovers of the present invention and Chinese herbal medicine mixed culture fermentation.
Embodiment 2: by slant preservation can produce antibacterial lipopeptid bacillus subtilis (
bacillus subtilis) in the LB fluid nutrient medium that cools after being linked into sterilizing of HL-1, in 37 DEG C, 150r/min cultivates 16h, be prepared into bacillus subtilis (
bacillus subtilis) HL-1 seed liquor is for subsequent use; It is for subsequent use after fresh shrimp head is rubbed; 60 mesh sieves are crossed for subsequent use after the Radix Astragali, the coptis and garden burnet being pulverized.Composite Chinese herbal (25 kilograms of Radixs Astragali+25 kilograms of coptiss+50 kilograms of garden burnets) mixing that 80 kilograms of shrimp heads rubbed and 100 kilograms are pulverized, and add 380 grams of glucose, 400 grams of potassium chloride, 315 grams of magnesium chlorides, 550 grams of dipotassium hydrogen phosphates mix together with 15 kg of water, obtain mixing after fermentation medium; Fermented and cultured after mixing is based on 121 DEG C of sterilizing 30min; Then add in the fermentation medium cooled after sterilizing 2 kilograms of bacillus subtilises (
bacillus subtilis) HL-1 seed liquor, stir and mix; Postvaccinal raw material is divided in the fermentation tray of the alcohol surface sterilization using 75%, thickness of feeding in tray is 4 centimetres, the wet gauze of material surface coverage sterilizing in tray, again tray is placed in the shelf top fermentation in the solid fermentation room of sterilizing, the temperature that controls environment is 32 DEG C, and fermentation time is 40h; After fermentation ends, by the product drying obtained after fermentation, after pulverizing and packaging, namely obtain the feed addictive finished product of a kind of aquatic product leftovers of the present invention and Chinese herbal medicine mixed culture fermentation.
Embodiment 3: by slant preservation can produce antibacterial lipopeptid bacillus subtilis (
bacillus subtilis) in the LB fluid nutrient medium that cools after being linked into sterilizing of HL-1, in 37 DEG C, 150r/min cultivates 16h, be prepared into bacillus subtilis (
bacillus subtilis) HL-1 seed liquor is for subsequent use; It is for subsequent use after scallop body is rubbed; 60 mesh sieves are crossed for subsequent use after being pulverized by cordate houttuynia.The scallop body that 120 kg is rubbed and 100 kilograms of cordate houttuynias mixing of pulverizing, and add 350 grams of glucose, 150 grams of potassium chloride, 300 grams of magnesium chlorides and, 500 grams of dipotassium hydrogen phosphates and mix together with 8 kg of water, obtain the fermentation medium after mixing; Mixed fermented and cultured is based on 121 DEG C of sterilizing 30min; Then add in the fermentation medium cooled after sterilizing 2 kilograms of bacillus subtilises (
bacillus subtilis) HL-1 seed liquor, stir and mix; Postvaccinal raw material is divided in the fermentation tray of the alcohol surface sterilization using 75%, thickness of feeding in tray is 6 centimetres, the wet gauze of material surface coverage sterilization treatment in tray, again tray is placed in the shelf top fermentation in the solid fermentation room of sterilizing, the temperature that controls environment is at 30 DEG C, and fermentation time is 45h; After fermentation ends, by the product drying obtained after fermentation, after pulverizing and packaging, namely obtain the feed addictive finished product of a kind of aquatic product leftovers of the present invention and Chinese herbal medicine mixed culture fermentation.
Embodiment 4: by slant preservation can produce antibacterial lipopeptid bacillus subtilis (
bacillus subtilis) in the LB fluid nutrient medium that cools after being linked into sterilizing of HL-1, in 37 DEG C, 150r/min cultivates 16h, be prepared into bacillus subtilis (
bacillus subtilis) HL-1 seed liquor is for subsequent use; It is for subsequent use after cod leftover bits and pieces is rubbed; 60 mesh sieves are crossed for subsequent use after being pulverized by granatum.By 110 kilograms rub cod leftover bits and pieces and 100 kilograms pulverize granatums mixing, and add 350 grams of glucose, 255 grams of potassium chloride, 280 grams of magnesium chlorides, 450 grams of dipotassium hydrogen phosphates mix together with 15 kg of water, obtain mixing after fermentation medium; Mixed fermented and cultured is based on 121 DEG C of sterilizing 30min; Then add in the fermentation medium cooled after sterilizing 1 kilogram of bacillus subtilis (
bacillus subtilis) HL-1 seed liquor, stir and mix; Postvaccinal raw material is divided in the fermentation tray of the alcohol surface sterilization using 75%, charging thickness is 8 centimetres, the wet gauze of material surface coverage sterilization treatment in tray, again tray is placed in the shelf top fermentation in the solid fermentation room of sterilizing, the temperature that controls environment is at 31 DEG C, and fermentation time is 46h; After fermentation ends, by the product drying obtained after fermentation, after pulverizing and packaging, namely obtain the feed addictive finished product of a kind of aquatic product leftovers of the present invention and Chinese herbal medicine mixed culture fermentation.
Embodiment 5: by slant preservation can produce antibacterial lipopeptid bacillus subtilis (
bacillus subtilis) in the LB fluid nutrient medium that cools after being linked into sterilizing of HL-1, in 37 DEG C, 150r/min cultivates 16h, be prepared into bacillus subtilis (
bacillus subtilis) HL-1 seed liquor is for subsequent use; It is for subsequent use after Harengula zunasi leftover bits and pieces is rubbed; By kuh-seng and broken rear mistake 60 mesh sieve of the coptis for subsequent use.Harengula zunasi leftover bits and pieces after 80 kilograms are rubbed and 100 kilograms of composite Chinese herbal (70 kilograms of kuh-sengs+30 kilograms of coptiss) mixing of pulverizing, and add 350 grams of glucose, 150 grams of potassium chloride, 290 grams of magnesium chlorides, 500 grams of dipotassium hydrogen phosphates mix together with 10 kg of water, obtain mixing after fermentation medium; Mixed fermented and cultured is based on 121 DEG C of sterilizing 30min; Then add in the fermentation medium cooled after sterilizing 1 kilogram of bacillus subtilis (
bacillus subtilis) HL-1 seed liquor, stir and mix; Postvaccinal raw material is divided in the fermentation tray of the alcohol surface sterilization using 75%, charging thickness is 4 centimetres, the wet gauze of material surface coverage sterilization treatment in tray, again tray is placed in the shelf top fermentation in the solid fermentation room of sterilizing, the temperature that controls environment is at 30 DEG C, and fermentation time is 45h; After fermentation ends, by the product drying obtained after fermentation, after pulverizing and packaging, namely obtain the feed addictive finished product of a kind of aquatic product leftovers of the present invention and Chinese herbal medicine mixed culture fermentation.
Embodiment 6: by slant preservation can produce antibacterial lipopeptid bacillus subtilis (
bacillus subtilis) in the LB fluid nutrient medium that cools after being linked into sterilizing of HL-1, in 37 DEG C, 150r/min cultivates 16h, be prepared into bacillus subtilis (
bacillus subtilis) HL-1 seed liquor is for subsequent use; It is for subsequent use after stripped tuna leftover bits and pieces is rubbed; 60 mesh sieves are crossed for subsequent use after smilax and honeysuckle being pulverized.Composite Chinese herbal (50 kilograms of smilaxs+50 kilograms of honeysuckles) mixing that 90 kilograms of stripped tuna leftover bits and pieces rubbed and 100 kilograms are pulverized, and add 480 grams of glucose, 280 grams of potassium chloride, 180 grams of magnesium chlorides, 580 grams of dipotassium hydrogen phosphates mix together with 13 kg of water, obtain mixing after fermentation medium; Mixed fermented and cultured is based on 121 DEG C of sterilizing 30min; Then add in the fermentation medium cooled after sterilizing 1 kilogram of bacillus subtilis (
bacillus subtilis) HL-1 seed liquor, stir and mix; Postvaccinal raw material is divided in the fermentation tray of the alcohol surface sterilization using 75%, charging thickness is 4 centimetres, the wet gauze of material surface coverage sterilization treatment in tray, again tray is placed in the shelf top fermentation in the solid fermentation room of sterilizing, the temperature that controls environment is at 32 DEG C, and fermentation time is 40h; After fermentation ends, by the product drying obtained after fermentation, after pulverizing and packaging, namely obtain the feed addictive finished product of a kind of aquatic product leftovers of the present invention and Chinese herbal medicine mixed culture fermentation.
Claims (7)
1. a preparation method for the feed addictive of aquatic product leftovers and Chinese herbal medicine mixed culture fermentation, is characterized in that: this preparation method comprises the steps:
(1) in the LB fluid nutrient medium cooled after the bacillus subtilis HL-1 that can produce antibacterial lipopeptid of slant preservation being linked into sterilizing, in 37 DEG C, 150r/min cultivates 16h, is prepared into bacillus subtilis HL-1 seed liquor for subsequent use;
(2) for subsequent use after fresh aquatic product leftovers being rubbed, cross 60 mesh sieves after being pulverized by Chinese herbal medicine for subsequent use;
(3) aquatic product leftovers of rubbing step (2) obtained and the rear Chinese herbal medicine crossing 60 mesh sieves of pulverizing are that the ratio of 0.5 ~ 1.2:1 mixes in mass ratio, and add nutrients and water mixing, obtain final moisture content and control the fermentation medium in 40% ~ 80% scope;
(4) fermented and cultured of mixing step (3) obtained is based on 121 DEG C of sterilizing 30min;
(5) fermentation medium after sterilizing step (4) obtained is cooled to room temperature, then accesses the bacillus subtilis HL-1 seed liquor prepared by step (1), and mixes;
(6) fermentation of raw material: the fermentation medium adding bacterium that step (5) obtains is fermented according to following method: fermentation medium is divided in the fermentation tray of having sterilized, charging thickness is 4cm ~ 8cm, the sterilized wet gauze of fermentation medium surface coverage in tray, again tray is placed in the shelf top fermentation in sterilized solid fermentation room, the temperature that controls environment is at 30 DEG C ~ 32 DEG C, and fermentation time is 40h ~ 50h;
(7) product handling system: fermentation ends, by the product drying obtained after fermentation, after pulverizing and packaging, can obtain the feed addictive finished product of a kind of aquatic product leftovers and Chinese herbal medicine mixed culture fermentation.
2. the preparation method of the feed addictive of a kind of aquatic product leftovers according to claim 1 and Chinese herbal medicine mixed culture fermentation, is characterized in that: bacillus subtilis HL-1 used in described step (1) is CGMCC No.10379 at the deposit number at China Committee for Culture Collection of Microorganisms's common micro-organisms center.
3. the preparation method of the feed addictive of a kind of aquatic product leftovers according to claim 1 and Chinese herbal medicine mixed culture fermentation, is characterized in that: the aquatic product leftovers in described step (2) is the one in leftovers of tilapia, shrimp head, scallop body, cod leftover bits and pieces, squid leftover bits and pieces, carp leftover bits and pieces, Harengula zunasi leftover bits and pieces, black carp leftover bits and pieces, silver carp leftover bits and pieces, grass carp leftover bits and pieces and stripped tuna leftover bits and pieces.
4. the preparation method of the feed addictive of a kind of aquatic product leftovers according to claim 1 and Chinese herbal medicine mixed culture fermentation, is characterized in that: the Chinese herbal medicine in described step (2) is a kind of or wherein two or more mixture in the Radix Astragali, honeysuckle, granatum, garden burnet, the coptis, cordate houttuynia, kuh-seng and smilax.
5. the preparation method of the feed addictive of a kind of aquatic product leftovers according to claim 1 and Chinese herbal medicine mixed culture fermentation, is characterized in that: the mixture that the nutrients in described step (3) is glucose, potassium chloride, magnesium chloride and dipotassium hydrogen phosphate form.
6. the preparation method of the feed addictive of a kind of aquatic product leftovers according to claim 1 and Chinese herbal medicine mixed culture fermentation, it is characterized in that: the nutraceutical addition in described step (3) accounts for fermentation medium gross mass percentages with the nutraceutical quality of adding, be respectively: glucose 0.01% ~ 5%, potassium chloride 0.01% ~ 5%, magnesium chloride 0.01% ~ 5%, dipotassium hydrogen phosphate 0.01% ~ 5%.
7. the preparation method of the feed addictive of a kind of aquatic product leftovers according to claim 1 and Chinese herbal medicine mixed culture fermentation, it is characterized in that: in described step (5), the inoculum concentration of bacillus subtilis HL-1 seed liquor accounts for fermentation medium gross mass percentages with the quality of the seed liquor of access, is 0.1% ~ 5%.
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| CN201510092596.7A CN104719635A (en) | 2015-03-02 | 2015-03-02 | Method for preparing feed additive mixed and fermented by aquatic product leftover and Chinese herbal medicine |
| CN201610106541.1A CN105746881A (en) | 2015-03-02 | 2016-02-27 | Method for preparing substituent of antibiotics for feed by fermenting Chinese herbal medicines through lipopeptide-producing bacillus subtilis |
| CN201610112485.2A CN105707453A (en) | 2015-03-02 | 2016-03-01 | Preparing method of feed antibiotic substitute rich in lipopeptid and Chinese herbal medicine effective ingredients |
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| CN201610106541.1A Pending CN105746881A (en) | 2015-03-02 | 2016-02-27 | Method for preparing substituent of antibiotics for feed by fermenting Chinese herbal medicines through lipopeptide-producing bacillus subtilis |
| CN201610112485.2A Pending CN105707453A (en) | 2015-03-02 | 2016-03-01 | Preparing method of feed antibiotic substitute rich in lipopeptid and Chinese herbal medicine effective ingredients |
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| CN201610112485.2A Pending CN105707453A (en) | 2015-03-02 | 2016-03-01 | Preparing method of feed antibiotic substitute rich in lipopeptid and Chinese herbal medicine effective ingredients |
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| CN109943502B (en) * | 2019-03-04 | 2022-02-01 | 青岛海洋生物医药研究院 | Probiotics, leavening agent and application in Chinese herbal medicine preparation |
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| CN102094054B (en) * | 2010-12-07 | 2013-04-10 | 南京农业大学 | Production method of Bacillus subtilis antimicrobial lipopeptide and application of Bacillus subtilis antimicrobial lipopeptide in piglet feeds |
| CN104719635A (en) * | 2015-03-02 | 2015-06-24 | 广东海洋大学 | Method for preparing feed additive mixed and fermented by aquatic product leftover and Chinese herbal medicine |
| CN105211634A (en) * | 2015-10-13 | 2016-01-06 | 延川县富源康茶业专业合作社 | A kind of regulant for animal's growth and preparation method thereof |
| CN105211616B (en) * | 2015-10-27 | 2020-01-07 | 广东海洋大学 | Comprehensive utilization method of prawn leftovers |
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- 2016-02-27 CN CN201610106541.1A patent/CN105746881A/en active Pending
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| CN105724764B (en) * | 2016-03-09 | 2019-10-29 | 广东海洋大学 | A method of antibacterial green feed additive for promoting growth is prepared using aquatic product protein fermentation |
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| CN105707453A (en) | 2016-06-29 |
| CN105746881A (en) | 2016-07-13 |
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