CN104082528B - A kind of feed addictive and preparation method thereof - Google Patents
A kind of feed addictive and preparation method thereof Download PDFInfo
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- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
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Abstract
The present invention discloses a kind of feed addictive and preparation method thereof, belongs to feed additive field.Described feed addictive comprises following parts by weight raw material: Astragalus Root P.E 20-35 parts; Radix Codonopsis extract 10-20 parts; Bupleurum extract 10-20 parts; Bacillus subtilis culture 10-25 parts.Product use amount of the present invention is few, stable performance, use safety, with other feed addictive all without incompatibility, adopt Chinese herbal medicine extract and microbial bacterial agent rational proportion, the method utilizing enzymolysis and alcohol dipping to extract improves the recovery rate of the beneficiating ingredient such as amino acid, polysaccharide, trace element in Chinese herbal medicine, coordinate the bacillus subtilis culture of high yield enzyme again, in effective promotion feed, Chinese herbal medicine beneficiating ingredient digests and assimilates; Also improve the energy of all feeds resource and the utilized value of protein simultaneously, enhance the production performance of livestock and poultry, stress the comprehensive benefit of performance and raising, improve animal daily gain and feed conversion rate, the use amount of medicament-saving.
Description
Technical field:
The invention belongs to feed additive field, high-efficiency feed additive particularly containing plurality of Chinese composition and preparation method thereof.
Background technology:
Along with the fast development of China's animal husbandry, the application of feed addictive is increasingly extensive, for ensure animal husbandry sound development, meet the demand of people to animal food and make a great contribution, but also bring series of problems simultaneously.Because most of additive belongs to the medicine of chemical synthesis class, as hormone, antibiotic etc., while raising livestock and poultry output, livestock products medicine residual increases, and directly endangers the health of the mankind; And in modern livestock and poultry cultivation process, in order to prevention and therapy Animal diseases, also the excessive antibiotic product of normal use guarantees animal health, but excessive antibiotic use often causes the quality of animal meat product and product thereof to decline and antibiotic resistance strengthens, and causes human health to be also subject to having a strong impact on of antibiotic problem by the transmission of food chain; People have recognized the negative effect that these chemical synthesis class additive, antibiotic etc. bring gradually.
Be derived from more natural natural materials and not only there is good nutritive peculiarity, also there is the effects such as antiviral, anti-inflammatory, anti-oxidant, conditioner body immunity function simultaneously, have broad application prospects; And Chinese herbal medicine is just with the mode of action of its uniqueness, good result, noresidue, without the resistance to the action of a drug and pollution-free and be subject to the favor of numerous scientific research personnel, manufacturer.
At present, the Chinese herbal feed additive major part of putting on market is pulvis or powder, and its production technology falls behind, and production equipment is simple and crude, process simply coarse, kind is single, and using dosage is generally bigger than normal, this not only adds product cost, waste medicine, and have impact on the nutrition-allocated proportion of feed, the existence of these problems also makes Chinese herbal feed additive in the market not meet the basic function principle of " micro-, efficient " this feed addictive, is difficult to realize industrialization, standardization.
Therefore, develop and there is scientific matching, and successful can realize the feed addictive of " trace, efficient ", for promoting that the development of animal husbandry has very important significance containing multiple Chinese herbal and crude drugs preparations.
Summary of the invention:
The technical problem that the present invention solves is to provide a kind of high-efficiency feed additive product and preparation method thereof.
A kind of feed addictive, comprises the raw material of following parts by weight: Astragalus Root P.E 20-35 parts; Radix Codonopsis extract 10-20 parts; Bupleurum extract 10-20 part; Bacillus subtilis culture 10-25 parts.
The production method of described feed addictive comprises the steps::
(1) preparation of Astragalus Root P.E: raw material astragalus membranaceus powder being broken to particle diameter is less than 2 millimeters, the water adding 3-6 times of weight in container mixes, and control temperature 70 DEG C-90 DEG C keeps 2-4h, be 5.5-6.8 by lactic acid adjust ph, be cooled to 45-60 DEG C, add mixed enzyme, enzymolysis 2-4h, add the mixture of mixed material 0.5-3 times of w ethanol and propyl alcohol, control temperature to 60 DEG C-78 DEG C, keeps 3-4h, filters; Filter vacuum concentrates postlyophilization.
Described mixed enzyme addition is 5-10% of mixed material gross weight.
The parts by weight of described mixed enzyme consist of: endo-beta-glucanase 15-25 part, zytase 10-15 part, pentosanase 10-15 parts, beta amylase 15-20 parts, acid protease 15-20 part.
The mass ratio of described ethanol and propyl alcohol is 1:1-1.2.
(2) preparation of Radix Codonopsis extract: raw material codonopsis pilosula powder being broken to particle diameter is less than 2 millimeters, the water adding 3-6 times of weight in container mixes, control temperature 70 DEG C-90 DEG C, keeps 2-4h, is down to 45-60 DEG C, be 5.5-6.8 by lactic acid adjust ph, add mixed enzyme, enzymolysis 2-4h, add the mixture of mixed material 0.5-3 times of w ethanol and propyl alcohol, control temperature to 60 DEG C-78 DEG C of maintenance 3-4h, filter; Filter vacuum concentrates postlyophilization.
Described mixed enzyme addition is the 5-10% of mixed material gross weight.
The parts by weight of described mixed enzyme consist of: endo-beta-glucanase 10-20 part, outer 1,4 beta-glucanase 10-20 part, beta-glucosidase 10-15 parts, zytase 15-20 part, pentosanase 15-20 part, neutral proteinase 10-15 parts.
The mass ratio of described ethanol and propyl alcohol is 1:1-1.5.
(3) preparation of bupleurum extract: added radix bupleuri 3-6 times of weight absolute ethyl alcohol Soakage extraction after radix bupleuri being pulverized 30-40 mesh sieves, control temperature 30-45 DEG C, adjusting temperature after 2-4 hours is 55-60 DEG C of 1-2 hour, and extract is concentrated, drying obtains ethanol extract; Add 75-85 DEG C of hot water in radix bupleuri residue after alcohol extract, hot water addition is 2-4 times of radix bupleuri residue weight, and processing time 30-50 minute, extracts 2-3 times continuously, by spraying dry after extract Vacuum Concentration, obtains hot water extract; Above-mentioned ethanol extract and hot water extract are merged drying and crushing, crosses 45 mesh sieves, obtain bupleurum extract.
(4) preparation of bacillus subtilis culture: cultivate bacillus subtilis from inclined-plane switching, the seed liquor after spreading cultivation step by step is transferred in fermentation tank, and control temperature is 37-42 DEG C, aerlbic culture 19-24 hours, and throughput is 2.0m
3/ minute; Ferment complete, after fermentation liquor plate-frame filtering, concentrated, smart filter, freeze drying, obtain bacillus subtilis culture.
The bacterial strain of product Thermostable α-Amylase provided by the invention is specially bacillus subtilis (Bacillussubtilis) Li-2013-02.This bacterial strain is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center (be called for short CGMCC, address is: No. 3, No. 1, North Star West Road, Chaoyang District, city of BeiJing, China institute) on July 15th, 2013, and preserving number is CGMCCNo.7926.
The comparatively ratio of greater inequality example of feed addictive of the present invention is: Astragalus Root P.E 25-30 parts; Radix Codonopsis extract 13-18 parts; Bupleurum extract 14-18 parts; Bacillus subtilis culture 15-20 parts.
The preparation method of described feed addictive is as follows: proportionally mixed by above-mentioned constitutive material, pack.
Beneficial effect:
The Chinese herb astragalus adopted in the present invention: containing the various trace elements such as saponin, sucrose, polysaccharide, several amino acids, folic acid and selenium, zinc, copper, there is invigorating qi for strengthening superficies, sharp water detumescence, pus draining and toxin expelling, enhanced machine body immunity function, protect the liver, anti-ageing, resisting stress, hypotensive effect.
Radix Codonopsis: have tonifying spleen and stomach and beneficial lung qi, beneficial gas is with the effect of enriching blood; Radix Codonopsis also has excitation to nervous system, can strengthen Abwehrkraft des Koepers; Can also peripheral vasodilation be made and reduce blood pressure, and can adrenal boosting be suppressed.
Radix bupleuri: the effect with antipyretic analgesic, has trivial solution heat effect to the caused heating such as typhoid fever, paratyphoid vaccine, Escherichia coli liquid, fermented milk, yeast, and animal normal body temperature can be made to decline; Radix bupleuri has calm effect, is used for the treatment of the diseases such as the insomnia and dreamful sleep that interior hot agitation causes; Radix bupleuri has antagonism to central stimulant, therefore has certain therapeutic action to cough; Radix bupleuri also infected by influenza, hepatitis viruse, vaccinia virus, I type poliomyelitis virus, herpesviral is also effective, can also make that phagocytic function strengthens, Natural killer activity strengthens, improve special viral antibody titre, improve the effect that lymphocyte turns core rate.
Feed addictive use amount of the present invention is few, stable performance, use safety, with other feed addictive all without incompatibility, adopt the Radix Astragali, Radix Codonopsis, bupleurum extract and bacillus subtilis culture rational proportion, the method utilizing enzymolysis and alcohol dipping to extract improves the amino acid in Chinese herbal medicine, polysaccharide, the recovery rate of the beneficiating ingredients such as trace element, utilize bacillus subtilis, free oxygen in rapid consumption alimentary canal environment, promote that useful anaerobic bacteria grows, and produce the organic acids such as lactic acid, reduce enteron aisle pH value, improve gut flora, and effectively promote digesting and assimilating of Chinese herbal medicine beneficiating ingredient in feed, simultaneously bacillus subtilis thalline can also the digestibility enzyme such as self synthetic proteins enzyme, amylase, jointly play a role with endogenous enzymes in alimentary canal, thus improve the energy of all feeds resource and the utilized value of protein, enhance the production performance of livestock and poultry, stress the comprehensive benefit of performance and raising, improve animal daily gain and feed conversion rate, save the use amount of feed.
Chinese herbal medicine in the present invention also has inhibitory action to the staphylococcus aureus of multiple coccus, bacillus and resistance, and bacillus subtilis thalline has growing of energy stimulating animal immune organ, activated lymphocyte, improve immunoglobulin (Ig) and antibody horizontal, strengthen the function of cellular immunity and humoral immunity, the subtilin produced in growth course, polymyxins, nystatin, the conditioned pathogen of gramicidins isoreactivity material to pathogenic bacteria or autogenous infection have obvious inhibitory action; Therefore, by Chinese herbal medicine extract and bacillus subtilis culture to the interaction of gut flora, significantly decreasing the intestines problem of animal, reduce antibiotic use amount, improve livestock and poultry body immunity, is natural green non-pollution animal feed.
Containing highly active amylase in the bacillus subtilis culture contained in product, amylase can provide for starch material in feed and effectively decompose utilization, improves food utilization efficiency; The result of use of the enzyme that fire resistant alpha-diastase can effectively improve.Viable bacteria in bacillus subtilis culture, as prebiotic components, also effectively can improve the health of intestine microenvironment, promotes the raising of animal health level.
Product of the present invention, to the level of production improving China's animal husbandry, improves effective utilization of feed resource, fully realizes aspects such as " micro-, efficient " and all has very high using value
Detailed description of the invention:
The following examples can make the present invention of those skilled in the art comprehend, but do not limit the present invention in any way.
Bacterial strain of the present invention screens acquisition by the wild mushroom of acid ground through ultraviolet mutagenesis repeatedly and nitrosoguanidine mutagenesis, and characteristic is that the enzyme activity of product Thermostable α-Amylase is high, heat-resisting, acid resistance is strong.
The Thermostable α-Amylase enzyme activity that bacterial strain produces is 30000-35000u/ml; Applicable temperature scope is 105-115 DEG C, optimal reactive temperature 110 DEG C, at 110 DEG C of enzymes complete stability alive; Being suitable for pH value in reaction scope is 3.0-7.0, and the enzyme complete stability alive when pH value is 3.0, optimal reaction pH value is 4.2.
The bacterial strain of product Thermostable α-Amylase provided by the invention is specially bacillus subtilis (Bacillussubtilis) Li-2013-02.This bacterial strain is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center (be called for short CGMCC, address is: No. 3, No. 1, North Star West Road, Chaoyang District, city of BeiJing, China institute) on July 15th, 2013, and preserving number is CGMCCNo.7926.
Described bacterial strain feature is as follows:
Described bacterial strain colony colour on solid plate is milky, and dry tack free is opaque, neat in edge, for having the aerobic bacteria of motility.Microscopy is elongated rod shape, and Gram's staining is positive.This bacterium can utilize citrate, and nitrate reductase, V-P test into the positive.
Described bacillus subtilis (Bacillussubtilis) Li-2013-02 is obtained through UV-LiCl-dithyl sulfate Mutation screening by the bacillus subtilis Li-2013 producing Thermostable α-Amylase, and concrete screening step is as follows:
(1) preparation of bacteria suspension
The mono-bacterium colony of Li-2013 grown after plate streaking is separated is accessed in seed culture medium, 100r/min, after 40 DEG C of cultivation 12h, after getting 1mL medium centrifugal, use brine twice, and resuspended with 9mL physiological saline.
(2) UV-LiCl-dithyl sulfate complex mutation
Bacteria suspension is placed in aseptic flat board, is 30cm in distance, stirs and irradiate 100s under the uviol lamp of power 15w.Bacterium liquid through irradiating is coated lithium chloride flat board after gradient dilution, and contrasts to be coated with flat board without the bacterium liquid dilution of ultraviolet irradiation.Above-mentioned coating is dull and stereotyped uniformly, wrap with the cloth of black or newspaper, put 40 DEG C and cultivate 48h, the flat board growing bacterium colony filters out hydrolysis circle choose preserve to inclined-plane with colony diameter ratio the maximum, bacteria suspension is mixed with after purifying, fully mix with dithyl sulfate stoste after gradient dilution, and in 40 DEG C of concussion process 40min, the bacterium liquid processed is coated lithium chloride flat board after gradient dilution.
(3) primary dcreening operation of high-yield strains
Above-mentioned coating is dull and stereotyped uniformly, and put 40 DEG C and cultivate 48h, on the flat board growing bacterium colony, primary dcreening operation goes out hydrolysis circle and chooses preserve to inclined-plane with colony diameter ratio the greater, obtains three strain bacterium Li-2013-01, Li-2013-02, Li-2013-03 after purifying
(4) shake flask fermentation sieves again
By the three strain bacterium Li-2013-01 obtained, Li-2013-02, Li-2013-03 carry out shake flask fermentation in the 250mL shaking flask containing 30mL fermentation medium, seed inoculum concentration 10% (V/V), 40 DEG C, 100r/min cultivates 72h, centrifuging and taking fermented supernatant fluid obtains crude enzyme liquid.
(5) enzyme activity determination
The definition of Mei Huo unit: 1mL crude enzyme liquid, in 105 DEG C, under pH4.2 condition, 1min liquefies 1mg soluble starch,
Be 1 enzyme activity unit, represent with U/mL.
After measured, bacterial strain Li-2013-02 is stable most superior strain, and enzyme is lived and reached 30000U/mL.
Described lithium chloride is dull and stereotyped: starch 1%, peptone 1%, (NH)
2sO
40.4%, K
2hPO
40.8%, CaCl
20.2%, lithium chloride 0.9%, agar 2%.
Described seed culture medium: dusty yeast 0.5%, peptone 1%, soluble starch 1%, NaCl1%.
Described fermentation medium: corn flour 5%-15%, beancake powder 4%-10%, (NH)
2sO
40.4%, K
2hPO
40.8%, CaCl
20.2%.
Described shake flask culture conditions: this bacterium in the 250mL shaking flask containing 30mL fermentation medium, inoculum concentration 10% (V/V), 100r/min, 40 DEG C of fermented and cultured 72h.
Described high-temperature resistant alpha-amylase, its zymologic property is as follows:
(1) this enzyme Acclimation temperature wider range, optimum temperature between 100-110 DEG C, and to be preserved below 110 DEG C, and temperature stability is better, and more than 110 DEG C to preserve long-time temperature stability poor.
(2) this enzyme optimal reaction pH value is 4.2.High enzyme vigor is all had, the enzyme complete stability alive when pH value is 3.0 between pH value 3.0-7.0.
(3) enzymatic activity: by mutant strain Li-2013-02 provided by the present invention, the Thermostable α-Amylase enzyme activity of preparation is 30000-35000U/ml.
Embodiment 1: preparation method is with example 3
A kind of feed addictive comprises the raw material of following parts by weight: Astragalus Root P.E 27 parts; Radix Codonopsis extract 15 parts; Bupleurum extract 16 parts; Bacillus subtilis culture 20 parts.
Embodiment 2:
The preparation of bacillus subtilis culture:
Adopt slant strains to spread cultivation step by step and obtain fermentation of bacillus subtilis liquid;
(1) first order seed is cultivated: bacillus subtilis slant strains accessed in 500 ml shake flasks, culture medium loading amount 100 milliliters, rotary shaker 180 revs/min, cultivation temperature 40 DEG C, incubation time 12 hours;
(2) secondary seed cultivate: by first order seed according to 10% inoculum concentration access in 500 milliliters of secondary seed shaking flasks, condition of culture is identical with first order seed;
(3) three grades of seed culture: secondary seed is accessed in 5000 milliliters of three grades of seed flask with 10% inoculum concentration, culture medium loading amount 1000 milliliters, rotary shaker 100 revs/min, cultivation temperature 40 DEG C, incubation time 12 hours;
(4) first class seed pot is cultivated: the first class seed pot being 150L with 10% inoculum concentration access total measurement (volume) by three grades of seeds, fermentation medium loading amount 100L, cultivation temperature 43 DEG C, mixing speed 100 revs/min, ventilation (V/V) 1:1, tank pressure 0.05Mpa, incubation time 15 hours;
(5) fermented and cultured: it is 1.5 tons of secondary seed tanks that first class seed pot bacterial classification is accessed total measurement (volume) with 10% inoculum concentration, fermentation medium loading amount 1 ton, condition of culture cultivation temperature 40 DEG C, mixing speed 100 revs/min, ventilation (V/V) 1:1.5, tank pressure 0.05Mpa, incubation time 24 hours.
(6) preparation of culture: ferment complete, obtains bacillus subtilis culture after fermentation liquor plate-frame filtering, concentrated, smart filter, freeze drying.
Described culture medium composition: glucose 6%, yeast extract 1%, peptone 0.2%, CaCO31%, pH6.8.
Described bacillus subtilis (Bacillussubtilis) preserving number is CGMCCNo.7926.
Embodiment 3
A kind of feed addictive comprises the raw material of following parts by weight: Astragalus Root P.E 35 parts; Radix Codonopsis extract 10 parts; Bupleurum extract 18 parts; Bacillus subtilis culture 10 parts.
The production method of described feed addictive comprises the steps:
(1) preparation of Astragalus Root P.E: raw material astragalus membranaceus powder being broken to particle diameter is less than 2 millimeters, the water adding 5 times of weight in container mixes, control temperature 80 DEG C, keeps 3h, is cooled to 55 DEG C, be 6 by lactic acid adjust ph, add mixed enzyme, enzymolysis 3h, add the mixture of mixed material 2 times of w ethanol and propyl alcohol, control temperature to 68 DEG C keeps 4h, filters; Filter vacuum concentrates postlyophilization.
Described mixed enzyme addition is 7% of mixed material gross weight.
The parts by weight of described mixed enzyme consist of: endo-beta-glucanase 20 parts, zytase 13 parts, pentosanase 12 parts, beta amylase 17 parts, acid protease 18 parts.
The mass ratio of described ethanol and propyl alcohol is 1:1.1.
(2) preparation of Radix Codonopsis extract: raw material codonopsis pilosula powder being broken to particle diameter is less than 2 millimeters, the water adding 5 times of weight in container mixes, control temperature 80 DEG C, keeps 3h, is cooled to 50 DEG C, be 6 by lactic acid adjust ph, add mixed enzyme, enzymolysis 3h, add the mixture of mixed material 3 times of w ethanol and propyl alcohol, control temperature to 68 DEG C keeps 3h, filters; Filter vacuum concentrates postlyophilization.
Described mixed enzyme addition is 7% of mixed material gross weight.
The parts by weight of described mixed enzyme consist of: endo-beta-glucanase 15 parts, outer 1,4 beta-glucanase 15 parts, beta-glucosidase 12 parts, zytase 18 parts, pentosanase 17 parts, neutral proteinase 12 parts.
The mass ratio of described ethanol and propyl alcohol is 1:1.
(3) preparation of bupleurum extract: added radix bupleuri 5 times of weight absolute ethyl alcohol Soakage extraction after radix bupleuri being pulverized 35 mesh sieves, control temperature 38 DEG C, adjust after 3 hours temperature be 60 DEG C 2 hours, extract is concentrated, drying obtains ethanol extract; Add 80 DEG C of hot water in radix bupleuri residue after alcohol extract, hot water addition is 3 times of radix bupleuri residue weight, 40 minutes processing times, extracts 2 times continuously, by spraying dry after extract Vacuum Concentration, obtains hot water extract; Above-mentioned ethanol extract and hot water extract are merged drying and crushing, crosses 45 mesh sieves, obtain bupleurum extract.
(4) bacillus subtilis culture preparation: cultivate bacillus subtilis from inclined-plane switching, the seed liquor after spreading cultivation step by step is transferred in fermentation tank, and control temperature is 40 DEG C, aerlbic culture 22 hours, and throughput is 2.0m
3/ minute; Ferment complete, after fermentation liquor plate-frame filtering, concentrated, smart filter, freeze drying, obtain bacillus subtilis culture.
Product result of use
The result of use test of example 3 feed addictive of the present invention in weanling pig
Test method:
Experimental animal selection plant average weight is the healthy weanling pig 120 of (7.16 ± 0.15) kg, and statistical analysis weight differences is not remarkable.Adopt single-factor Randomized Designs, by 120 healthy weanling pigs, by male and female half and half, be divided into 2 groups (control group and test group), often organize 6 repetitions, each repetition 10 pigs.Test group adds the obtained product of the present invention of embodiment 1, and control group does not add product of the present invention.Feed continuously 30 days, compared with control group, result of the test shows, in weanling pig daily ration, add this product, and the daily gain of piglet improves a lot than control group; Feedstuff-meat ratio has obvious reduction; Diarrhea rate reduces 85.7%.
The result of use test of example 1 feed addictive of the present invention in milking sow
The feeding experiment of 21 days has by a definite date been carried out on certain pig farm.Test adopts single factor test contrast design, random selecting 30 healthy, farrowing head number is close with birth counterpoise, parity is the milking sow of 2 or 3 tires, be divided at random 2 groups (i.e. test group and control groups), often group 15 repetitions.Wherein: test group daily ration adds this product be prepared into by embodiment 1, and control group adds common like product.Test shows: test group can improve 49.5% than control group weight of weaning litter, and piglet head daily gain improves 33.6%, and diarrhea rate reduces 57.3%, and the death rate reduces by 70.3%.
The above results shows that product of the present invention can improve sow and piglet body immunity, reduces antibiotic dosage, increases cultivation quality and benefits.
Claims (6)
1. a feed addictive, comprises the raw material of following parts by weight: Astragalus Root P.E 20-35 part; Radix Codonopsis extract 10-20 part; Bupleurum extract 10-20 part; Bacillus subtilis culture 10-25 part; The preparation method of described bacillus subtilis culture is as follows: cultivate bacillus subtilis from inclined-plane switching, the seed liquor after spreading cultivation step by step is transferred in fermentation tank, and control temperature is 37-42 DEG C, aerlbic culture 19-24 hour, and throughput is 2.0m
3/ minute; Ferment complete, after fermentation liquor plate-frame filtering, concentrated, smart filter, freeze drying, obtain bacillus subtilis culture; Described bacillus subtilis (Bacillussubtilis) preserving number is CGMCCNo.7926; The preparation method of Astragalus Root P.E is as follows:
Raw material astragalus membranaceus powder being broken to particle diameter is less than 2 millimeters, the water adding 3-6 times of weight in container mixes, control temperature 70 DEG C-90 DEG C, keeps 2-4h, be cooled to 45-60 DEG C, be 5.5-6.8 by lactic acid adjust ph, add mixed enzyme, enzymolysis 2-4h, add the mixture of mixed material 0.5-3 times of w ethanol and propyl alcohol, control temperature 60 DEG C-78 DEG C, keeps 3-4h, filters; Filter vacuum concentrates postlyophilization;
Described mixed enzyme addition is the 5-10% of mixed material gross weight;
The parts by weight of described mixed enzyme consist of: endo-beta-glucanase 15-25 part, zytase 10-15 part, pentosanase 10-15 part, beta amylase 15-20 part, acid protease 15-20 part;
The mass ratio of described ethanol and propyl alcohol is 1: 1-1.2.
2. feed addictive as claimed in claim 1, it is characterized in that, the preparation method of Radix Codonopsis extract is as follows:
Raw material codonopsis pilosula powder being broken to particle diameter is less than 2 millimeters, the water adding 3-6 times of weight in container mixes, control temperature 70 DEG C-90 DEG C, keeps 2-4h, be down to 45-60 DEG C, be 5.5-6.8 by lactic acid adjust ph, add mixed enzyme, enzymolysis 2-4h, add the mixture of mixed material 0.5-3 times of w ethanol and propyl alcohol, control temperature to 60 DEG C-78 DEG C, keeps 3-4h, filters; Filter vacuum concentrates postlyophilization;
Described mixed enzyme addition is the 5-10% of mixed material gross weight;
The parts by weight of described mixed enzyme consist of: endo-beta-glucanase 10-20 part, outer 1,4 beta-glucanase 10-20 part, beta-glucosidase 10-15 part, zytase 15-20 part, pentosanase 15-20 part, neutral proteinase 10-15 part;
The mass ratio of described ethanol and propyl alcohol is 1: 1-1.5.
3. feed addictive as claimed in claim 1 or 2, it is characterized in that, the preparation method of bupleurum extract is as follows:
Added radix bupleuri 3-6 times of weight absolute ethyl alcohol Soakage extraction after radix bupleuri being pulverized 30-40 mesh sieve, adjusting temperature after control temperature 30-45 DEG C, 2-4 hour is 55-60 DEG C, 1-2 hour, and extract is concentrated, drying obtains ethanol extract; Add 75-85 DEG C of hot water in radix bupleuri residue after alcohol extract, hot water addition is 2-4 times of radix bupleuri residue weight, and processing time 30-50 minute, extracts 2-3 time continuously, by spraying dry after extract Vacuum Concentration, obtain hot water extract; Above-mentioned ethanol extract and hot water extract are merged drying and crushing, crosses 45 mesh sieves, obtain bupleurum extract.
4. feed addictive as claimed in claim 1 or 2, comprises the raw material of following parts by weight: Astragalus Root P.E 35 parts; Radix Codonopsis extract 10 parts; Bupleurum extract 18 parts; Bacillus subtilis culture 10 parts.
5. feed addictive as claimed in claim 1 or 2, the production method of described feed addictive comprises the steps:
The constitutive material of described feed addictive is proportionally mixed, packs;
(1) preparation of Astragalus Root P.E: raw material astragalus membranaceus powder being broken to particle diameter is less than 2 millimeters, the water adding 5 times of weight in container mixes, control temperature 80 DEG C, keeps 3h, is cooled to 55 DEG C, be 6 by lactic acid adjust ph, add mixed enzyme, enzymolysis 3h, add the mixture of mixed material 2 times of w ethanol and propyl alcohol, control temperature to 68 DEG C keeps 4h, filters; Filter vacuum concentrates postlyophilization;
Described mixed enzyme addition is 7% of mixed material gross weight;
The parts by weight of described mixed enzyme consist of: endo-beta-glucanase 20 parts, zytase 13 parts, pentosanase 12 parts, beta amylase 17 parts, acid protease 18 parts;
The mass ratio of described ethanol and propyl alcohol is 1: 1.1;
(2) preparation of Radix Codonopsis extract: raw material codonopsis pilosula powder being broken to particle diameter is less than 2 millimeters, the water adding 5 times of weight in container mixes, control temperature 80 DEG C, keeps 3h, is cooled to 50 DEG C, be 6 by lactic acid adjust ph, add mixed enzyme, enzymolysis 3h, add the mixture of mixed material 3 times of w ethanol and propyl alcohol, control temperature to 68 DEG C keeps 3h, filters; Filter vacuum concentrates postlyophilization;
Described mixed enzyme addition is 7% of mixed material gross weight;
The parts by weight of described mixed enzyme consist of: endo-beta-glucanase 15 parts, outer 1,4 beta-glucanase 15 parts, beta-glucosidase 12 parts, zytase 18 parts, pentosanase 17 parts, neutral proteinase 12 parts;
The mass ratio of described ethanol and propyl alcohol is 1: 1;
(3) preparation of bupleurum extract: added radix bupleuri 5 times of weight absolute ethyl alcohol Soakage extraction after radix bupleuri being pulverized 35 mesh sieves, control temperature 38 DEG C, adjust after 3 hours temperature be 60 DEG C 2 hours, extract is concentrated, drying obtains ethanol extract; Add 80 DEG C of hot water in radix bupleuri residue after alcohol extract, hot water addition is 3 times of radix bupleuri residue weight, 40 minutes processing times, extracts 2 times continuously, by spraying dry after extract Vacuum Concentration, obtains hot water extract; Above-mentioned ethanol extract and hot water extract are merged drying and crushing, crosses 45 mesh sieves, obtain bupleurum extract;
(4) bacillus subtilis culture preparation: cultivate bacillus subtilis from inclined-plane switching, the seed liquor after spreading cultivation step by step is transferred in fermentation tank, and control temperature is 40 DEG C, aerlbic culture 22 hours, and throughput is 2.0m
3/ minute; Ferment complete, after fermentation liquor plate-frame filtering, concentrated, smart filter, freeze drying, obtain bacillus subtilis culture.
6. preparation as arbitrary in claim 1-5 as described in a kind of preparation method of feed addictive, described preparation method is as follows: proportionally mixed by the constitutive material of described feed addictive, pack.
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Effective date of registration: 20160302 Address after: 211600 Jinhu health Road, Jiangsu, No. 32, No. Patentee after: JINHU SHUNXING FARM Address before: 211600 Jinhu health Road, Jiangsu, No. 32, No. Patentee before: Zhu Jianguo |
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| CB03 | Change of inventor or designer information | ||
| CB03 | Change of inventor or designer information |
Inventor after: Sun Xiaofei Inventor after: Wang Wenjuan Inventor after: Wang Baohua Inventor before: Zhu Jianguo |
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| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20171218 Address after: No. 19, No. 19, No. 12, Zhongguancun South Avenue, Haidian District, Beijing Patentee after: Beijing Durun Science and Technology Co., Ltd. Address before: 211600 Jinhu health Road, Jiangsu, No. 32, No. Patentee before: JINHU SHUNXING FARM |