CN104719632A - Feed additive and preparation method thereof - Google Patents
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Abstract
The invention discloses a feed additive and a preparation method thereof, belonging to the field of feed additives. The feed additive comprises the following raw materials in parts by weight: 5-10 parts of a yeast cell wall extract, 25-36 parts of a bacillus subtilis culture, 5-10 parts of an astragalus membranaceus extract, 5-8 parts of a radix bupleuri extract and 2-6 parts of fructo-oligose. The feed additive is small in product use amount, stable in property and safe to use and has no incompatibility with other feed additives, traditional Chinese herbal medicine extracts are reasonably matched with microbial agents, and are matched together with the bacillus subtilis culture with high enzyme yield, digestion and absorption of beneficial components in the traditional Chinese herbal medicines in feed can be effectively prompted, and meanwhile, the energy of various feed resources and the available values of protein can be increased, the production property and the stress property of livestock can be improved, the comprehensive feeding benefits of the livestock can be increased, the daily gain of the livestock and the feed conversion rate can be increased, and the use amount of medicines can be reduced.
Description
Technical field:
The invention belongs to feed additive field, high-efficiency feed additive particularly containing plurality of Chinese composition and preparation method thereof.
Background technology:
Along with the fast development of China's animal husbandry, the application of feed addictive is increasingly extensive, for ensure animal husbandry sound development, meet the demand of people to animal food and made tremendous contribution, but also bring series of problems simultaneously.Because most of additive belongs to the medicine of chemical synthesis class, as hormone, antibiotic etc., while raising livestock and poultry output, livestock products medicine residual increases, and directly endangers the health of the mankind.In modern livestock and poultry cultivation process, in order to prevention and therapy Animal diseases, also the excessive antibiotic product of normal use guarantees animal health.But excessive antibiotic use often causes the quality of animal meat product and product thereof to decline and antibiotic resistance strengthens, and causes human health to be also subject to having a strong impact on of antibiotic problem by the transmission of food chain.People have recognized the negative effect that these chemical synthesis class additive, antibiotic etc. bring gradually.Be derived from more natural natural materials and not only there is good nutritive peculiarity, also there is the effects such as antiviral, anti-inflammatory, anti-oxidant, conditioner body immunity function simultaneously, have broad application prospects.And Chinese herbal medicine is just with the mode of action of its uniqueness, good result, noresidue, without the resistance to the action of a drug and pollution-free and be subject to the favor of numerous scientific research personnel, manufacturer.
At present, the Chinese herbal feed additive major part of putting on market is pulvis or powder.These product processes fall behind, and production equipment is simple and crude, and process simply coarse, kind is single, and using dosage is generally bigger than normal.Not only increase product cost, waste medicine, and have impact on the nutrition-allocated proportion of feed, the existence of these problems also makes Chinese herbal feed additive in the market not meet the basic function principle of " micro-, efficient " this feed addictive, is difficult to realize industrialization, standardization.
Therefore, develop the complex Chinese herbal medicine with scientific matching, successful, the feed addictive of " micro-, efficient " can not only be realized, and for promoting that the development of animal husbandry also has very important significance.
Summary of the invention:
The technical problem that the present invention solves is to provide a kind of high-efficiency feed additive product and preparation method thereof.
A kind of feed addictive, comprises the raw material of following parts by weight: yeast cell wall extract 5-10 part, bacillus subtilis culture 25-36 part, Astragalus Root P.E 5-10 part; Bupleurum extract 5-8 part; FOS 2-6 part.
The production method of described feed addictive comprises the steps:
(1) preparation of Astragalus Root P.E: it is less than 2 millimeters that raw material astragalus membranaceus powder is broken to particle diameter, and the water adding 3-6 times of weight in container mixes, control temperature 70 DEG C-90 DEG C, keep 2-4h, be 5.5-6.8 by lactic acid adjust ph, be cooled to 45-60 DEG C, add mixed enzyme, enzymolysis 2-4h, add the mixture of mixed material 0.5-3 times of w ethanol and propyl alcohol, control temperature to 60 DEG C-78 DEG C, keeps 3-4h, filter, filter vacuum concentrates postlyophilization.
Described mixed enzyme addition is the 5-10% of mixed material gross weight.
The parts by weight of described mixed enzyme consist of: endo-beta-glucanase 15-25 part, zytase 10-15 part, pentosanase 10-15 part, beta amylase 15-20 part, acid protease 15-20 part.
The mass ratio of described ethanol and propyl alcohol is 1: 1-1.2.
(2) preparation of bupleurum extract: added radix bupleuri 3-6 times of weight absolute ethyl alcohol Soakage extraction after radix bupleuri being pulverized 30-40 mesh sieve, control temperature 30-45 DEG C, adjusting temperature after 2-4 hour is 55-60 DEG C of maintenance 1-2 hour, and extract is concentrated, drying obtains ethanol extract.Add 75-85 DEG C of hot water in radix bupleuri residue after alcohol extract, hot water addition is 2-4 times of radix bupleuri residue weight, and processing time 30-50 minute, extracts 2-3 time continuously, by spraying dry after extract Vacuum Concentration, obtain hot water extract.Above-mentioned ethanol extract and hot water extract are merged drying and crushing, crosses 45 mesh sieves, obtain bupleurum extract.
(3) preparation of bacillus subtilis culture: cultivate bacillus subtilis from inclined-plane switching, the seed liquor after spreading cultivation step by step is transferred in fermentation tank, and control temperature is 37-42 DEG C, aerlbic culture 19-24 hour, and throughput is 2.0m
3/ minute.Ferment complete, after fermentation liquor plate-frame filtering, concentrated, smart filter, freeze drying, obtain bacillus subtilis culture.
The preparation method of described feed addictive is as follows: yeast cell wall extract and FOS pulverized 50-60 mesh sieve, and above-mentioned crushed material and bacillus subtilis culture, Astragalus Root P.E, bupleurum extract are proportionally mixed to get feed addictive.
The bacterial classification that the present invention adopts is as follows:
Bacillus subtilis (Bacillus subtilis) Li-2013-02, this bacterial strain is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on July 15th, 2013 and (is called for short CGMCC, address is: No. 3, No. 1, North Star West Road, Chaoyang District, city of BeiJing, China institute), preserving number is CGMCC No.7926.
Described strain characteristic is that the enzyme activity of product Thermostable α-Amylase is high, heat-resisting, acid resistance is strong.
Thermostable α-Amylase enzyme activity prepared by described bacterial strain is 30000-35000u/ml; Applicable temperature scope is 105-115 DEG C, optimal reactive temperature 110 DEG C, at 110 DEG C of enzymes complete stability alive; Being suitable for pH value in reaction scope is 3.0-7.0, and the enzyme complete stability alive when pH value is 3.0, optimal reaction pH value is 4.2.
Described bacterial strain feature is as follows:
Described bacterial strain colony colour on solid plate is milky, and dry tack free is opaque, neat in edge, for having the aerobic bacteria of motility.Microscopy is elongated rod shape, and Gram's staining is positive.This bacterium can utilize citrate, and nitrate reductase, V-P test into the positive.
Described bacillus subtilis (Bacillus subtilis) Li-2013-02 is produced Thermostable α-Amylase bacillus subtilis Li-2013 by a strain obtains through UV-LiCl-dithyl sulfate Mutation screening, and concrete screening step is as follows:
(1) preparation of bacteria suspension
The mono-bacterium colony of Li-2013 grown after plate streaking is separated is accessed in seed culture medium, 100r/min, after 40 DEG C of cultivation 12h, after getting 1mL medium centrifugal, use brine twice, and resuspended with 9mL physiological saline.
(2) UV-LiCl-dithyl sulfate complex mutation
Bacteria suspension is placed in aseptic flat board, is 30cm in distance, stirs and irradiate 100s under the uviol lamp of power 15w.Bacterium liquid through irradiating is coated lithium chloride flat board after gradient dilution, and contrasts to be coated with flat board without the bacterium liquid dilution of ultraviolet irradiation.Above-mentioned coating is dull and stereotyped uniformly, wrap with the cloth of black or newspaper, put 40 DEG C and cultivate 48h, the flat board growing bacterium colony filters out hydrolysis circle choose preserve to inclined-plane with colony diameter ratio the maximum, bacteria suspension is mixed with after purifying, fully mix with dithyl sulfate stoste after gradient dilution, and in 40 DEG C of concussion process 40min, the bacterium liquid processed is coated lithium chloride flat board after gradient dilution.
(3) primary dcreening operation of high-yield strains
Above-mentioned coating is dull and stereotyped uniformly, and put 40 DEG C and cultivate 48h, on the flat board growing bacterium colony, primary dcreening operation goes out hydrolysis circle and chooses preserve to inclined-plane with colony diameter ratio the greater, obtains three strain bacterium Li-2013-01, Li-2013-02, Li-2013-03 after purifying.
(4) shake flask fermentation sieves again
By the three strain bacterium Li-2013-01 obtained, Li-2013-02, Li-2013-03 carry out shake flask fermentation in the 250mL shaking flask containing 30mL fermentation medium, seed inoculum concentration 10% (V/V), 40 DEG C, 100r/min cultivates 72h, centrifuging and taking fermented supernatant fluid obtains crude enzyme liquid.
(5) enzyme activity determination
The definition of Mei Huo unit: 1mL crude enzyme liquid, in 105 DEG C, under pH4.2 condition, 1min liquefies 1mg soluble starch, is 1 enzyme activity unit, represents with U/mL.
After measured, bacterial strain Li-2013-02 is stable most superior strain, and enzyme is lived and reached 30000U/mL.
Described lithium chloride is dull and stereotyped: starch 1%, peptone 1%, (NH)
2sO
40.4%, K
2hPO
40.8%, CaCl
20.2%, lithium chloride 0.9%, agar 2%.
Described seed culture medium: dusty yeast 0.5%, peptone 1%, soluble starch 1%, NaCl1%.
Described fermentation medium: corn flour 5%-15%, beancake powder 4%-10%, (NH)
2sO
40.4%, K
2hPO
40.8%, CaCl
20.2%.
Described shake flask culture conditions: this bacterium in the 250mL shaking flask containing 30mL fermentation medium, inoculum concentration 10% (V/V), 100r/min, 40 DEG C of fermented and cultured 72h.
Described high-temperature resistant alpha-amylase, its zymologic property is as follows:
(1) this enzyme Acclimation temperature wider range, optimum temperature between 100-110 DEG C, and to be preserved below 110 DEG C, and temperature stability is better, and more than 110 DEG C to preserve long-time temperature stability poor.
(2) this enzyme optimal reaction pH value is 4.2.High enzyme vigor is all had, the enzyme complete stability alive when pH value is 3.0 between pH value 3.0-7.0.
(3) enzymatic activity: by mutant strain Li-2013-02 provided by the present invention, the Thermostable α-Amylase enzyme activity of preparation is 30000-35000U/ml.
Beneficial effect:
The Chinese herb astragalus adopted in the present invention: containing the various trace elements such as saponin, sucrose, polysaccharide, several amino acids, folic acid and selenium, zinc, copper, there is invigorating qi for strengthening superficies, sharp water detumescence, pus draining and toxin expelling, enhanced machine body immunity function, protect the liver, anti-ageing, resisting stress, hypotensive effect.
Radix bupleuri: the effect with antipyretic analgesic, has trivial solution heat effect to the caused heating such as typhoid fever, paratyphoid vaccine, Escherichia coli liquid, fermented milk, yeast, and animal normal body temperature can be made to decline; Radix bupleuri has calm effect, is used for the treatment of the diseases such as the insomnia and dreamful sleep that interior hot agitation causes; Radix bupleuri has antagonism to central stimulant, therefore has certain therapeutic action to cough; Radix bupleuri also infected by influenza, hepatitis viruse, vaccinia virus, I type poliomyelitis virus, herpesviral is also effective, can also make that phagocytic function strengthens, Natural killer activity strengthens, improve special viral antibody titre, improve the effect that lymphocyte turns core rate.
FOS refers to that 2 ~ 5 fructosyls are chain link, the end group being chain with a glucosyl group, with fructosyl → fructose connecting key for main body framework links the carbohydrate formed.Namely refer to that 1 ~ 4 fructosyl is connected to the mixture of ketose (GF2), Nystose (GF3), GF4 (GF4) and sugarcane fruit six sugar (GF5) that the D-Fructose base of sucrose is formed with β-2,1 key.FOS is a kind of excellent water-soluble dietary fiber, and Bifidobacterium can also be impelled to breed rapidly, thus suppresses the growth of harmful bacteria, safeguards that gut flora balances; In enteron aisle can natural synthetic vitamin B1, B2, B6, B12, nicotinic acid and folic acid, thus improve the function of enteron aisle, improve immunity and premunition.In yeast cell wall extract, the functional form carbohydrate of yeast cells outer wall in conjunction with multiple mycotoxin, can improve animal health by absorbing on poisonous substance and pathogen to cell membrane.
Yeast cell wall extract take saccharomyces cerevisiae as raw material, and through the class fungal extract that the techniques such as breaking-wall cell, enzymolysis, separating-purifying and drying are refined, finished product is generally the light grey powder to Dark grey.Research confirms, yeast cell wall is divided into 3 layers: outer is manna oligosacchride and protein conjugate, and intermediate layer is β-(1,3), β-(1,6) glucan, and internal layer is chitin.Its special configuration can form special complementary structure with multiple mycotoxin, thus is combined firmly with multiple mycotoxin, and is discharged outside animal body by enteron aisle.
Feed addictive use amount of the present invention is few, stable performance, use safety, with other feed addictive all without incompatibility.Adopt the Radix Astragali, bupleurum extract and bacillus subtilis culture rational proportion, the method utilizing enzymolysis and alcohol dipping to extract improves the recovery rate of the beneficiating ingredient such as amino acid, polysaccharide, trace element in Chinese herbal medicine.Utilize bacillus subtilis, consume rapidly the free oxygen in digestion intestinal environment, promote that useful anaerobic bacteria grows, and produce the organic acids such as lactic acid, enteron aisle pH value can be reduced, improve gut flora, thus effectively promote digesting and assimilating of Chinese herbal medicine beneficiating ingredient in feed.Bacillus subtilis thalline can also the digestibility enzyme such as self synthetic proteins enzyme, amylase.Jointly play a role with endogenous enzymes in alimentary canal, thus improve the energy of all feeds resource and the utilized value of protein, enhance the production performance of livestock and poultry, stress the comprehensive benefit of performance and raising, improve animal daily gain and feed conversion rate, saved the use amount of feed.
Chinese herbal medicine in the present invention also has inhibitory action to the staphylococcus aureus of multiple coccus, bacillus and resistance.And bacillus subtilis thalline has growing of energy stimulating animal immune organ, activated lymphocyte, improve immunoglobulin (Ig) and antibody horizontal, strengthen the function of cellular immunity and humoral immunity.The subtilin produced in growth course, polymyxins, nystatin, gramicidins isoreactivity material, have obvious inhibitory action to the conditioned pathogen of pathogenic bacteria or autogenous infection.Therefore, by Chinese herbal medicine extract and bacillus subtilis culture to the interaction of gut flora, considerably reduce the intestines problem of animal, reduce antibiotic use amount, improve livestock and poultry body immunity, improve the security of animal meat product, improve the food utilization efficiency of animal, improving raise benefit, is natural green non-pollution animal feed.
Product of the present invention, to the level of production improving China's animal husbandry, improves effective utilization of feed resource, fully realizes aspects such as " micro-, efficient " and all has very high using value.
Detailed description of the invention:
The following examples can make the present invention of those skilled in the art comprehend, but do not limit the present invention in any way.
Embodiment 1:
The preparation of bacillus subtilis culture:
Adopt slant strains to spread cultivation step by step and obtain fermentation of bacillus subtilis liquid;
(1) first order seed is cultivated: bacillus subtilis slant strains accessed in 500 ml shake flasks, culture medium loading amount 100 milliliters, rotary shaker 180 revs/min, cultivation temperature 40 DEG C, incubation time 12 hours;
(2) secondary seed cultivate: by first order seed according to 10% inoculum concentration access in 500 milliliters of secondary seed shaking flasks, condition of culture is identical with first order seed;
(3) three grades of seed culture: secondary seed is accessed in 5000 milliliters of three grades of seed flask with 10% inoculum concentration, culture medium loading amount 1000 milliliters, rotary shaker 100 revs/min, cultivation temperature 40 DEG C, incubation time 12 hours;
(4) first class seed pot is cultivated: the first class seed pot being 150L with 10% inoculum concentration access total measurement (volume) by three grades of seeds, fermentation medium loading amount 100L, cultivation temperature 43 DEG C, mixing speed 100 revs/min, ventilation (V/V) 1: 1, tank pressure 0.05MPa, incubation time 15 hours;
(5) fermented and cultured: it is 1.5 tons of secondary seed tanks that first class seed pot bacterial classification is accessed total measurement (volume) with 10% inoculum concentration, fermentation medium loading amount 1 ton, cultivation temperature 40 DEG C, mixing speed 100 revs/min, ventilation (V/V) 1: 1.5, tank pressure 0.05MPa, incubation time 24 hours.
(6) preparation of culture: ferment complete, obtains bacillus subtilis culture after fermentation liquor plate-frame filtering, concentrated, smart filter, freeze drying.
Described culture medium composition: glucose 6%, yeast extract 1%, peptone 0.2%, CaCO
31%, all the other are water, pH6.8.
Described bacillus subtilis (Bacillus subtilis) preserving number is CGMCC No.7926.
A kind of feed addictive comprises the raw material of following parts by weight: yeast cell wall extract 7 parts, bacillus subtilis culture 20 parts, Astragalus Root P.E 6 parts; FOS 5 parts; Aforementioned proportion is weight fraction ratio; Bacillus subtilis (Bacillussubtilis) Li-2013-02 preserving number is CGMCC No.7926.
Embodiment 2:
A kind of feed addictive comprises the raw material of following parts by weight: yeast cell wall extract 8 parts, bacillus subtilis culture 30 parts, Astragalus Root P.E 9 parts; Bupleurum extract 7 parts; FOS 4 parts, aforementioned proportion is weight fraction ratio.Bacillus subtilis (Bacillus subtilis) Li-2013-02 preserving number is CGMCC No.7926.
Embodiment 3:
Feedstuff additive product production method described in example 2: yeast cell wall extract and FOS pulverized 60 mesh sieves, is proportionally mixed to get feed addictive by above-mentioned crushed material and bacillus subtilis culture, Astragalus Root P.E, bupleurum extract.
The preparation of Astragalus Root P.E:
Raw material astragalus membranaceus powder being broken to particle diameter is less than 2 millimeters, the water adding 5 times of weight in container mixes, and control temperature 85 DEG C keeps 3h, be 6.5 by lactic acid adjust ph, be cooled to 52 DEG C, add mixed enzyme, enzymolysis 3h, add the mixture of mixed material 2 times of w ethanol and propyl alcohol, control temperature to 75 DEG C, keeps 4h, filters; Filter vacuum concentrates postlyophilization.
Described mixed enzyme addition is 7% of mixed material gross weight.
The parts by weight of described mixed enzyme consist of: endo-beta-glucanase 20 parts, zytase 10 parts, pentosanase 12 parts, beta amylase 18 parts, acid protease 17 parts.
The mass ratio of described ethanol and propyl alcohol is 1: 1.1.
The preparation of bupleurum extract:
Added radix bupleuri 5 times of weight absolute ethyl alcohol Soakage extraction after radix bupleuri being pulverized 40 mesh sieves, control temperature 40 DEG C, adjusting temperature after 3 hours is 57 DEG C of maintenances 2 hours, and extract is concentrated, drying obtains ethanol extract; Add 85 DEG C of hot water in radix bupleuri residue after alcohol extract, hot water addition is 3 times of radix bupleuri residue weight, 45 minutes processing times, extracts 3 times continuously, by spraying dry after extract Vacuum Concentration, obtains hot water extract; Above-mentioned ethanol extract and hot water extract are merged drying and crushing, crosses 45 mesh sieves, obtain bupleurum extract.
The preparation of bacillus subtilis culture: cultivate bacillus subtilis from inclined-plane switching, the seed liquor after spreading cultivation step by step is transferred in fermentation tank, and control temperature is 38 DEG C, aerlbic culture 23 hours, and throughput is 2.0m
3/ minute; Ferment complete, after fermentation liquor plate-frame filtering, concentrated, smart filter, freeze drying, obtain bacillus subtilis culture.
Embodiment 4
Product result of use:
The embodiment of the present invention 2 feed addictive result of use is tested
Test method:
Select age in days, healthy sow that body weight is close, piglet and each 50 of boar, be divided into all at random 2 groups (test group and control groups), test group uses product of the present invention, and control group does not add product of the present invention; The addition of product of the present invention is the 0.5-1.5% of feed consumption, feeds continuously 30 days.Compared with control group, use invention feed addictive can obtain following effect:
(1) number born of sow on average increases by 0.7;
(2) boar sperm number increases by 20% ~ 25%, improves conception rate;
(3), before and after childbirth, prevention of sow constipation number of times reduces more than 35%;
(4) weanling pig weight ratio control group weight average increases by 9%;
(5) antibiotic usage amount decreases 80-90% the piglet phase, and diarrhea rate reduces 85%, and the death rate reduces 70%;
The above results shows that product of the present invention can improve sow, piglet and boar body immunity, reduces antibiotic dosage, increases cultivation quality and benefits.Feed addictive of the present invention is of many uses, not only uses and meat swine rearing, is also applicable to the raising of chicken, duck, ox, sheep and aquatic livestock.
Claims (6)
1. a feed addictive, is characterized in that, comprises the raw material of following parts by weight: yeast cell wall extract 5-10 part, bacillus subtilis culture 25-36 part, Astragalus Root P.E 5-10 part; Bupleurum extract 5-8 part; FOS 2-6 part; Described bacillus subtilis preserving number is CGMCC No.7926.
2. a kind of feed addictive according to claim 1, it is characterized in that, the preparation method of bacillus subtilis culture is as follows:
(1) first order seed is cultivated: bacillus subtilis slant strains accessed in 500 ml shake flasks, culture medium loading amount 100 milliliters, rotary shaker 180 revs/min, cultivation temperature 40 DEG C, incubation time 12 hours;
(2) secondary seed cultivate: by first order seed according to 10% inoculum concentration access in 500 milliliters of secondary seed shaking flasks, condition of culture is identical with first order seed;
(3) three grades of seed culture: secondary seed is accessed in 5000 milliliters of three grades of seed flask with 10% inoculum concentration, culture medium loading amount 1000 milliliters, rotary shaker 100 revs/min, cultivation temperature 40 DEG C, incubation time 12 hours;
(4) first class seed pot is cultivated: the first class seed pot being 150L with 10% inoculum concentration access total measurement (volume) by three grades of seeds, fermentation medium loading amount 100L, cultivation temperature 43 DEG C, mixing speed 100 revs/min, ventilation (V/V) 1: 1, tank pressure 0.05MPa, incubation time 15 hours;
(5) fermented and cultured: it is 1.5 tons of secondary seed tanks that first class seed pot bacterial classification is accessed total measurement (volume) with 10% inoculum concentration, fermentation medium loading amount 1 ton, cultivation temperature 40 DEG C, mixing speed 100 revs/min, ventilation (V/V) 1: 1.5, tank pressure 0.05MPa, incubation time 24 hours;
(6) preparation of culture: ferment complete, obtains bacillus subtilis culture after fermentation liquor plate-frame filtering, concentrated, smart filter, freeze drying;
Described culture medium composition: glucose 6%, yeast extract 1%, peptone 0.2%, CaCO
31%, all the other are water, pH6.8.
3. a kind of feed addictive according to claim 1, it is characterized in that, the preparation method of Astragalus Root P.E is as follows: raw material astragalus membranaceus powder being broken to particle diameter is less than 2 millimeters, the water adding 3-6 times of weight in container mixes, control temperature 70 DEG C-90 DEG C, keeps 2-4h, be cooled to 45-60 DEG C, be 5.5-6.8 by lactic acid adjust ph, add mixed enzyme, enzymolysis 2-4h, add the mixture of mixed material 0.5-3 times of w ethanol and propyl alcohol, control temperature 60 DEG C-78 DEG C, keeps 3-4h, filters; Filter vacuum concentrates postlyophilization;
Described mixed enzyme addition is the 5-10% of mixed material gross weight;
The parts by weight of described mixed enzyme consist of: endo-beta-glucanase 15-25 part, zytase 10-15 part, pentosanase 10-15 part, beta amylase 15-20 part, acid protease 15-20 part;
The mass ratio of described ethanol and propyl alcohol is 1: 1-1.2.
4. a kind of feed addictive according to claim 1, it is characterized in that, the preparation of bupleurum extract: added radix bupleuri 3-6 times of weight absolute ethyl alcohol Soakage extraction after radix bupleuri being pulverized 30-40 mesh sieve, control temperature 30-45 DEG C, adjusting temperature after 2-4 hour is 55-60 DEG C of maintenance 1-2 hour, and extract is concentrated, drying obtains ethanol extract; Add 75-85 DEG C of hot water in radix bupleuri residue after alcohol extract, hot water addition is 2-4 times of radix bupleuri residue weight, and processing time 30-50 minute, extracts 2-3 time continuously, by spraying dry after extract Vacuum Concentration, obtain hot water extract; Above-mentioned ethanol extract and hot water extract are merged drying and crushing, crosses 45 mesh sieves, obtain bupleurum extract.
5. a kind of feed addictive according to claim 1; It is characterized in that, comprise the raw material of following parts by weight: yeast cell wall extract 8 parts, bacillus subtilis culture 30 parts, Astragalus Root P.E 9 parts; Bupleurum extract 7 parts; FOS 4 parts; Aforementioned proportion is weight fraction ratio; Bacillus subtilis preserving number is CGMCC No.7926.
6. according to the preparation method of the arbitrary described a kind of feed addictive of claim 1 to 5, described preparation method is as follows: yeast cell wall extract and FOS pulverized 50-60 mesh sieve, and above-mentioned crushed material and bacillus subtilis culture, Astragalus Root P.E, bupleurum extract are proportionally mixed to get feed addictive.
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| CN102630825A (en) * | 2012-05-09 | 2012-08-15 | 邵素英 | Compound plant extract feed additive |
| CN103382228A (en) * | 2013-07-31 | 2013-11-06 | 瑞普（天津）生物药业有限公司 | Preparation method for high-activity astragalus polysaccharide |
| CN103404830A (en) * | 2013-08-01 | 2013-11-27 | 宁夏红山河食品有限公司 | Hot pot condiment and preparation method thereof |
| CN103609858A (en) * | 2013-11-20 | 2014-03-05 | 宁夏天地经纬电力设备工程有限公司 | Feed additive and preparation method thereof |
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Application publication date: 20150624 |