CN104706678A - Coated lactic acid bacterial microcapsule preparation method - Google Patents
Coated lactic acid bacterial microcapsule preparation method Download PDFInfo
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- CN104706678A CN104706678A CN201310683006.9A CN201310683006A CN104706678A CN 104706678 A CN104706678 A CN 104706678A CN 201310683006 A CN201310683006 A CN 201310683006A CN 104706678 A CN104706678 A CN 104706678A
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Abstract
The present invention discloses a coated lactic acid bacterial microcapsule preparation method, wherein lactic acid bacteria and a pill production auxiliary material are subjected to mixing vulcanization granulation, and then an enteric coating material is used to repeatedly coat so as to prepare the coated lactic acid bacterial microcapsule. With the prepared coated lactic acid bacterial microcapsule of the present invention, the lactic acid bacteria are protected, such that the lactic acid bacteria can safely enter the intestinal tract through the gastric juice, and can be rapidly disintegrated in the intestinal tract and occupy the fixed planting site so as to inhibit the growth of the pathogenic bacteria. The coated lactic acid bacterial microcapsule has excellent storage stability at a room temperature, further has metal ion tolerance and high temperature tolerance, and is suitable for wide application in low-profit feed industry and livestock and poultry breeding production.
Description
Invention field
The present invention relates to a kind of preparation field of active microorganism, specifically relate to a kind of bag by the preparation method of lactobacillus micro-capsule.
Background technology
Antibiotic had once played important function in livestock and poultry cultivation, but the drawbacks such as its drug resistance diffusion brought, drug residue and ecological disruption are also more and more obvious.Along with the reinforcement of people's Consciousness of food security, produce green livestock products crystalline substance in the urgent need to using green feed additive.Lactic acid bacteria class additive for microbe feedstuff is wherein very important one.Lactobacillus is the resident probiotics in animal body, and it can a word used in person's names resisting pathogenic microbes, regulates animal gut microflora balance; Activated immune system, enhancing immunity; In addition, lactobacillus can also prevent livestock and poultry diarrhea, improve poultry weightening finish, plays important physiological function in intestinal.
But because most lactobacillus does not produce spore, resistance is poor, and lactobacillus is easy to inactivation, so had a strong impact on its use value.Such as, the pH of gastric juice is usually below 2.5, and the lactobacillus that this acidity makes the overwhelming majority be ingested is destroyed, and millionth lactobacillus of only having an appointment can live arrival large intestine.When lactobacillus is added on granulate in feedstuff time, common feed granulating temperature is 80 ~ C, only within 5 minutes, can make at this temperature lactobacillus lose 70% ~ 80%.In addition, in Feed Manufacturing and livestock and poultry cultivation, for ease of using, usually lactobacillus is directly added in complete feedstuff or additive premix, because both all exists a large amount of trace element metal ions (as Cu
2+, Fe
2+, Mn
2+, Zn
2+and Ca
2+), the material such as vitamins (as choline chloride) and antibiotic, all can by a series of redox reaction, acid-base value change, change survival and vigor that the modes such as lactobacillus existence microenvironment have influence on lactobacillus.
In order to overcome above problem, solve usually through strengthening the use amount of lactobacillus in feed manufacturing and livestock and poultry breeding industry, but this is not the basic method of dealing with problems, be only a kind of temporarily, remedial measure that larger cost price need be paid.The technology comparatively advocating now use adopts coating measure to lactobacillus, and preparation lactic acid bacteria capsule, protects lactobacillus, thus reduces the impact of outer bound pair lactobacillus.As described in Japan Patent JP9241434, this technology have employed the high temperature higher than 55 DEG C in coating process, sublethal damage is caused to lactobacillus, namely adopts the lactobacillus of this technology coatings can grow in nutritious culture medium, but can not in intestinal field planting and propagation.In Chinese patent CNl276009A, also disclose method lactobacillus being adopted to secondary coating, this method improves the stomach juice-resistant of lactobacillus, but what this method adopted is release controlling coating material, make lactobacillus promptly can not discharge in intestinal and occupy field planting site, thus be unfavorable for the growth suppressing pathogen.This embodiment in poultry production is more obvious, because the intestinal of poultry is very short, only has 3 ~ 4 times that body is long, thus lactobacillus can rapid release in intestinal very necessary.
Meanwhile, the method is not protected the heat-resisting quantity of lactobacillus, shelf-stable.In sum, in these technology existing or obtained lactic acid bacteria capsule, lactic bacteria activity is low, or lactobacillus can not promptly discharge in intestinal, and cost is all higher, is thus unsuitable for the extensive use in the feed industry and livestock and poultry cultivation production of slight diarrhea.
Summary of the invention
The object of the invention is to overcome lactic bacteria activity in the obtained lactic acid bacteria capsule of prior art low, can not promptly discharge in intestinal, and the damage of the multiple harmful factor such as trace element metal ion, choline chloride, antibiotic contained in feedstuff can not be tolerated preferably, the high temperature in feed granulating process can not be tolerated, and the defect that cost is all higher, thus provide that a kind of activity is high, shelf-stable, stability are high, preparation cost is low, can safety gastric juice and the lactobacillus micro-capsule of rapid disintegrate after entering intestinal.
For solving the problem, bag provided by the present invention is by the preparation method of lactobacillus micro-capsule, and it comprises the following steps:
(1) lactobacillus dope is prepared:
By the lactobacillus be separated in healthy animal intestinal, cultivate in the culture medium after access sterilizing, then the lactic acid bacteria culture solution obtained carried out centrifugalize, with sterile saline by be separated obtain precipitate---bacterium mud is diluted to containing 10
9~ 10
11the mixed liquor of cfu/ml viable lactic acid bacteria, and add precipitate---the defatted milk powder of bacterium mud gross weight 3.5 ~ 9.5%, obtains lactobacillus dope;
(2) sulfuration pelletize:
To be equipped with in the pill pot of the rotation of the Fluidizinggraincoatingdrier of pill adjuvant, the lactobacillus dope that spraying process (1) is obtained under the state of pill adjuvant in boiling, after l2-28min, stop the spraying of lactobacillus dope, continue boiling and rotate pill pot 6-9 minute, obtain the spherical particles of diameter 1.1 ~ 1.9mm, in this spherical particles, content of lactic acid bacteria accounts for 22 ~ 29% of spherical particles gross weight;
The process conditions of the Fluidizinggraincoatingdrier used are: compressed air consumption is 0.25 ~ 0.55ml/min, hermetic seal adjust blood pressure is 0.14 ~ 0.18Mpa, atomizing pressure is 0.17 ~ 0.21Mpa, electric current adjust blood pressure is 0.25 ~ 0.55Mpa, material spray speed is 5.1 ~ 14.5ml/min, material spray inlet temperature is 25.5 ~ 94.5 DEG C, and outlet temperature is normal temperature and pressure;
(3) bag quilt:
The spherical particles that step (2) obtains is put into the coating pan of Fluidizinggraincoatingdrier, from coating pan import, to spray into concentration be respectively 1.1 ~ 5.1wt% wall material solution and concentration is simultaneously 1.1 ~ 3.1wt%CaCl
2: solution, material spray inlet temperature is 65.5 ~ 74.5 DEG C, and material spray speed is after 2 ~ 20ml/min, 12-29 minute, just the outlet in Fluidizinggraincoatingdrier obtain through the diameter of bag quilt be the bag of 2.1 ~ 2.9mm by lactobacillus micro-capsule, its outlet temperature is 32 ~ 48 DEG C.
Preferably, described pill adjuvant is a kind of, two or more the mixture in sucrose, maltodextrin, microcrystalline Cellulose, corn starch, glucose or protein sugar.
Preferably, described wall material is sodium alginate, CaCl
2with the one in carrageenan, soybean protein isolate, water-insoluble vegetable oil, two or more mixture.
Preferably, in described step (2), the spraying of lactobacillus dope is 2 times.
Preferably, described water-insoluble vegetable oil is oleum lini, Oleum Gossypii semen, Oleum Brassicae campestris, soybean salad oil, Oleum Helianthi or Semen Maydis oil.
Preferably, described lactobacillus, for tame through certain oxytolerant, has compared with the Lactobacillus plantarum of high viability, streptococcus faecalis, bacillus acidophilus, Lactobacillus fermenti or lactobacillus casei in aerobic operation process.
The invention has the beneficial effects as follows: by the bag obtained by the present invention by lactobacillus micro-capsule have active high, cost is low, high temperature resistant, acidproof, resistance to metal ion and as feed additive can safety gastric juice and in animal intestinal rapid disintegrate release lactobacillus, breed and occupy field planting site, suppress the efficiency of animal pathogenic bacteria growing high, be suitable for the extensive use in the feed industry and livestock and poultry cultivation production of slight diarrhea.
Detailed description of the invention
Embodiment 1
Use a kind of culture medium, it comprises component A, B, C and D, and wherein, described component A is one or more mixture in Carnis Bovis seu Bubali cream or peptone, yeast extract, skim milk/powder, soybean peptide, tryptone, Semen sojae atricolor powder; Described B component is one or more mixture in lactose, glucose, tomato juice, malt extract; Described component C is one or more mixture in tween 80, cysteine, Radix Dauci Sativae juice, potato juice, large Sucus Allii Fistulosi, Pleurotus ostreatus juice; Described component D is NaCI, KH
2p0
4, dibasic ammonium citrate, sodium acetate, MgS0
4in one or more mixture, by this culture medium sterilizing 15 ~ 30min at 121 DEG C, be cooled to room temperature.
From healthy animal intestinal, be separated to a strains of lactic acid bacteria (e.g., Lactobacillus plantarum), access above-mentioned culture medium, cultivate 12 hours at 25-42 DEG C; By culture fluid under 4000r/min condition, centrifugal 30min, obtains lactobacillus bacterium mud;
Diluted by this lactobacillus bacterium mud with sterile saline, add the defatted milk powder of lactobacillus bacterium mud gross weight 5%, obtaining viable bacteria concentration is 10
10the lactobacillus concentrated solution of cfu/ml.
Adopt FLP type Fluidizinggraincoatingdrier, according to sucrose: maltodextrin: microcrystalline Cellulose: the pill adjuvant 2kg that 5:4:1 (weight ratio) forms drops in pill pot, spray into above-mentioned lactobacillus concentrated solution simultaneously, the process conditions of the Fluidizinggraincoatingdrier that the part by weight of pill adjuvant and lactobacillus concentrated solution uses for 1:2 are: compressed air consumption is 0.4m3/min, hermetic seal adjust blood pressure is 0.17Mpa, atomizing pressure is 0.18Mpa, electric current adjust blood pressure is 0.4Mpa, spraying charging rate is 10ml/min, spraying inlet temperature and outlet temperature are room temperature, start FLP type Fluidizinggraincoatingdrier, observe pill situation by equipment form, through 15min, the ball-type lactic acid bacterium particle of obtained diameter 1.1 ~ 1.9mm.
Start coating device, be 70 ~ C in inlet temperature, outlet temperature is at 45 DEG C, sprays into the wall material solution of 2wt% sodium alginate, 1wt% carrageenan, 0.5wt% soybean salad oil and 0.4wt% soybean protein isolate, sprays into 2wt%CaCl from another shower nozzle simultaneously
2solution, charging rate is respectively 8ml/min and 4ml/min, obtained diameter be the bag of 2.1 ~ 2.9 by lactobacillus micro-capsule, its content of lactic acid bacteria accounts for 22% of microcapsule gross weight.
Embodiment 2
From healthy animal intestinal, be separated to a strains of lactic acid bacteria (e.g., streptococcus faecalis), in the culture medium of access described in embodiment 1, cultivate 12 hours at 25 ~ 42 DEG C; By culture fluid under 4000r/min condition, centrifugal 30min, obtains lactobacillus bacterium mud; Diluted by this lactobacillus bacterium mud with sterile saline, add the defatted milk powder of lactobacillus bacterium mud gross weight 3%, obtaining viable bacteria concentration is 10
9the lactobacillus concentrated solution of cfu/mL.
Adopt FLP type Fluidizinggraincoatingdrier, by microcrystalline Cellulose: sucrose: corn starch: the pill adjuvant 2kg that 2:3:5 (weight ratio) forms drops in pill pot, spray into above-mentioned lactobacillus concentrated solution simultaneously, the part by weight of pill adjuvant and lactobacillus concentrated solution is 1:2, carries out twice spraying; The process conditions of the Fluidizinggraincoatingdrier used are: compressed air consumption is 0.25m
3/ min, hermetic seal adjust blood pressure is 0.14Mpa, and atomizing pressure is 0.17Mpa,
Electric current adjust blood pressure is 0.25Mpa, and spraying charging rate is 5.1ml/min, and spraying import pathogenic wind-warm is 50 DEG C, and outlet temperature is room temperature; Start FLP type Fluidizinggraincoatingdrier, observe pill situation by equipment form, through 10min, the ball-type lactic acid bacterium particle of obtained diameter 1.1 ~ 1.9mm.
Start coating device, be 65 DEG C in inlet temperature, outlet temperature is at 30 DEG C, sprays into the wall material solution of 3wt% sodium alginate, 0.6wt% soybean salad oil and 0.5wt% soybean protein isolate, sprays into 1wt%CaCl from another shower nozzle simultaneously
2solution, charging rate is respectively 4ml/in and 2ml/min, and obtained diameter is the lactobacillus micro-capsule of 2.1-2.9mm, and its content of lactic acid bacteria accounts for 25% of microcapsule gross weight.Embodiment 3
From healthy animal intestinal, be separated to a strains of lactic acid bacteria (e.g., bacillus acidophilus can certainly be Lactobacillus fermenti or lactobacillus casei), in the culture medium of access described in embodiment 1, cultivate 12 hours at 25 ~ 42 DEG C; By culture fluid under 4000r/min condition, centrifugal 30min, obtains lactobacillus bacterium mud; Diluted by this lactobacillus bacterium mud with sterile saline, add the defatted milk powder of lactobacillus bacterium mud gross weight 10%, obtaining viable bacteria concentration is 10
11the lactobacillus concentrated solution of cfu.
Adopt FLP type Fluidizinggraincoatingdrier, according to sucrose: maltodextrin: protein sugar: the pill adjuvant 2kS that 2:3:4 (weight ratio) forms drops in pill pot, spray into above-mentioned lactobacillus concentrated solution simultaneously, the part by weight of pill adjuvant and lactobacillus concentrated solution is 1:3, carries out three sprayings; The process conditions of the Fluidizinggraincoatingdrier used are: compressed air consumption is 0.6m3/min, and hermetic seal adjust blood pressure is 0.20Mpa, and atomizing pressure is 0.22Mpa, electric current adjust blood pressure is 0.6Mpa, spraying charging rate is 15mVmin, and spraying import pathogenic wind-warm is 95 DEG C, and outlet temperature is room temperature; Start FLP type Fluidizinggraincoatingdrier, observe pill situation by equipment form, through 30min, the ball-type lactic acid bacterium particle of obtained diameter 1.1 ~ 1.9mm.Start coating device, be 75 DEG C in inlet temperature, outlet temperature is under 50'C, sprays into 5wt% sodium alginate wall material solution, sprays into 3wt%CaCl from another shower nozzle simultaneously
2solution, charging rate is respectively 20ml/min and 10mi/rain, obtained diameter be the bag of 2.1 ~ 2.9mm by lactobacillus micro-capsule, its content of lactic acid bacteria accounts for 30% of microcapsule gross weight.
Embodiment 4
The lactobacillus micro-capsule of preparation in embodiment 1 ~ 3 is carried out in simulated gastric fluid and simulated intestinal fluid the test of stomach juice-resistant performance.
By above-mentioned lactobacillus micro-capsule 5g, respectively in the simulated gastric fluid of 45ml two kinds of different pH, at 37 DEG C, hatch 2h, then proceed in the simulated intestinal fluid of different pH and process 2h, observe the disintegrate situation of microcapsule, the results are shown in table 1.Measure the viable count of lactobacillus, be calculated as follows the survival rate of micro encapsulation lactobacillus, the results are shown in table 2.
Survival rate: (before process viable count)/(after process viable count) X100%
Table 1, the lactobacillus micro-capsule disintegrate situation in simulated gastric fluid and simulated intestinal fluid
The survival rate of lactobacillus in simulated gastric fluid and simulated intestinal fluid of table 2, micro encapsulation
Comparative example is the data being selected from Chinese patent CNl276009A, and its treatment conditions are: in simulated gastric fluid, process 1h, then process 2h in simulated intestinal fluid; And lactobacillus micro-capsule of the present invention processes 2 hours (being longer than comparative example) respectively in gastric juice and intestinal juice, its survival rate is still higher than comparative example.From the result of table 1 and table 2; lactobacillus micro-capsule provided by the invention has carried out coated for protecting to lactobacillus; making it can the acid stronger gastric juice of safety; improve the resistance of lactobacillus; and enter after in intestinal, can disintegrate rapidly, occupy field planting site; and then breed and play its function, thus produce the effect of the growth suppressing pathogen.
The result of table 2 also proves that the stomach juice-resistant of the lactobacillus micro-capsule that lactobacillus micro-capsule provided by the invention provides than existing patent is stronger, even if the processing time comparatively long 1h of reference product in simulated gastric fluid, Lactobacillus Survival is still apparently higher than the survival rate of reference product.This be the present invention compared with existing patent, one of obvious advantage had.
Embodiment 5
The lactobacillus micro-capsule of preparation in embodiment 1-3 is stored 60 days at normal temperatures, measures the survival condition of lactobacillus, the results are shown in Table 3.
The survival rate (%) of micro encapsulation lactobacillus under table 3, storage at normal temperature condition
As shown in Table 3, lactobacillus micro-capsule provided by the invention is highly stable at normal temperatures, and lactic bacteria activity still can be kept after 60 days to be greater than 60%.
Embodiment 6
By in embodiment 1 ~ 3 preparation lactobacillus micro-capsule at normal temperatures with 1% chicken, pig premix mixing storage 60
My god, measure the survival condition of lactobacillus, the results are shown in Table 4 and table 5.
Table 4, to mix Lactobacillus Survival situation of change (%) when storing with the piglet premix containing high concentration trace elements metal ion
Table 5, to mix Lactobacillus Survival situation of change (%) when storing with the chicken premix material containing higher concentration trace element metal ion
Due to the mineral element (as ferrum, copper, zinc, cobalt, manganese) containing high dose in chicken and pig premix, choline chloride and vitamin (female IIVA, VB race) etc., all can there is a series of redox reaction and acid-base value change in them, thus produce certain impact to lactobacillus.The salt of these high concentrations all can make somatic cells dewater simultaneously, causes the physiological drying of thalline and then death.From table 4 and table 5, micro encapsulation lactobacillus provided by the invention and premix material mixing storage 60 days, Lactobacillus Survival is still more than 20%, and the product of non-micro encapsulation was when storage 60 days,
Can't detect viable bacteria.This result demonstrates microencapsulated products provided by the invention and can effectively prevent feedstuff Minerals salt and vitamins to the infringement of lactobacillus.And because the copper ion concentration in 1% high-copper piglet premix is than 1% chicken
Copper ion concentration in premix material is about high 15 times, therefore in 1% pig premix the loss speed of lactobacillus apparently higher than the loss speed in 1% chicken premix material.
Embodiment 7
Granulated under different pelleting temperature by the lactobacillus micro-capsule of preparation in embodiment 1 ~ 3, the survival condition of lactobacillus is in table 6.
Table 6, pelleting temperature are on the impact [lg (cfu/g)] of microorganism fodder activity
Lg (cfu/g) is the logarithm value of living cells quantity in every gram of feedstuff.As can be seen from Table 6, granulation can cause very big damage to the survival of lactobacillus, and microencapsulated products provided by the invention can significantly improve the survival rate of lactobacillus.Lactobacillus of granulating at 85 DEG C still has more than 3.87 orders of magnitude. viable bacteria exist.
The above is only preferred embodiment of the present invention, and be not restriction the present invention being made to other form, any those skilled in the art may utilize the technology contents of above-mentioned announcement to be changed or be modified as the Equivalent embodiments of equivalent variations.But everyly do not depart from technical solution of the present invention content, any simple modification, equivalent variations and the remodeling done above embodiment according to technical spirit of the present invention, still belong to the protection domain of technical solution of the present invention.
Claims (6)
1. bag is by a preparation method for lactobacillus micro-capsule, and it comprises the following steps:
(1) lactobacillus dope is prepared:
By the lactobacillus be separated in healthy animal intestinal, cultivate in the culture medium after access sterilizing, then the lactic acid bacteria culture solution obtained carried out centrifugalize, with sterile saline by be separated obtain precipitate---bacterium mud is diluted to containing 10
9~ 10
11the mixed liquor of cfu/ml viable lactic acid bacteria, and add precipitate---the defatted milk powder of bacterium mud gross weight 3.5 ~ 9.5%, obtains lactobacillus dope;
(2) sulfuration pelletize:
To be equipped with in the pill pot of the rotation of the Fluidizinggraincoatingdrier of pill adjuvant, the lactobacillus dope that spraying process (1) is obtained under the state of pill adjuvant in boiling, after l2-28min, stop the spraying of lactobacillus dope, continue boiling and rotate pill pot 6-9 minute, obtain the spherical particles of diameter 1.1 ~ 1.9mm, in this spherical particles, content of lactic acid bacteria accounts for 22 ~ 29% of spherical particles gross weight;
The process conditions of the Fluidizinggraincoatingdrier used are: compressed air consumption is 0.25 ~ 0.55ml/min, hermetic seal adjust blood pressure is 0.14 ~ 0.18Mpa, atomizing pressure is 0.17 ~ 0.21Mpa, electric current adjust blood pressure is 0.25 ~ 0.55Mpa, material spray speed is 5.1 ~ 14.5ml/min, material spray inlet temperature is 25.5 ~ 94.5 DEG C, and outlet temperature is normal temperature and pressure;
(3) bag quilt:
The spherical particles that step (2) obtains is put into the coating pan of Fluidizinggraincoatingdrier, from coating pan import, to spray into concentration be respectively 1.1 ~ 5.1wt% wall material solution and concentration is simultaneously 1.1 ~ 3.1wt%CaCl
2: solution, material spray inlet temperature is 65.5 ~ 74.5 DEG C, and material spray speed is after 2 ~ 20ml/min, 12-29 minute, just the outlet in Fluidizinggraincoatingdrier obtain through the diameter of bag quilt be the bag of 2.1 ~ 2.9mm by lactobacillus micro-capsule, its outlet temperature is 32 ~ 48 DEG C.
2. bag according to claim 1 is by the preparation method of lactobacillus micro-capsule, it is characterized in that described pill adjuvant is a kind of, two or more mixture in sucrose, maltodextrin, microcrystalline Cellulose, corn starch, glucose or protein sugar.
3. bag according to claim 1 is by the preparation method of lactobacillus micro-capsule, it is characterized in that described wall material is sodium alginate, CaCl
2with the one in carrageenan, soybean protein isolate, water-insoluble vegetable oil, two or more mixture.
4. bag according to claim 1 is by the preparation method of lactobacillus micro-capsule, it is characterized in that: in described step (2), the spraying of lactobacillus dope is 2 times.
5. bag according to claim 1 is by the preparation method of lactobacillus micro-capsule, it is characterized in that: described water-insoluble vegetable oil is oleum lini, Oleum Gossypii semen, Oleum Brassicae campestris, soybean salad oil, Oleum Helianthi or Semen Maydis oil.
6. bag according to claim 1 is by the preparation method of lactobacillus micro-capsule, it is characterized in that: described lactobacillus, for tame through certain oxytolerant, has compared with the Lactobacillus plantarum of high viability, streptococcus faecalis, bacillus acidophilus, Lactobacillus fermenti or lactobacillus casei in aerobic operation process.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106085864A (en) * | 2016-06-15 | 2016-11-09 | 天津农学院 | A kind of Aquatic product feeding micro encapsulation compound micro-ecological preparation and preparation method |
| CN106544299A (en) * | 2016-11-04 | 2017-03-29 | 中北大学 | A kind of composite bacteria agent capable of prevention ruminant oxalism and preparation method and application |
| CN106820155A (en) * | 2016-12-30 | 2017-06-13 | 江西益盟科技有限公司 | Lactic bacteria composition for regulating intestinal canal flora and strengthen immunity and preparation method thereof |
| CN107136322A (en) * | 2017-07-03 | 2017-09-08 | 青岛玛斯特生物技术有限公司 | Special-purpose protecting agent and its application are sprayed after a kind of aquatic feeds |
| CN109717481A (en) * | 2018-12-27 | 2019-05-07 | 广州智特奇生物科技股份有限公司 | A kind of preparation process of coating probiotics |
| CN110521857A (en) * | 2019-08-19 | 2019-12-03 | 南京师盛生态环境研究院有限公司 | A kind of preparation method of high density resistance lactobacillus micro-capsule |
| CN112244153A (en) * | 2020-08-29 | 2021-01-22 | 广州大北农农牧科技有限责任公司 | Preparation method of double-layer coated lactic acid bacteria microcapsule |
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2013
- 2013-12-14 CN CN201310683006.9A patent/CN104706678A/en not_active Withdrawn
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106085864A (en) * | 2016-06-15 | 2016-11-09 | 天津农学院 | A kind of Aquatic product feeding micro encapsulation compound micro-ecological preparation and preparation method |
| CN106544299A (en) * | 2016-11-04 | 2017-03-29 | 中北大学 | A kind of composite bacteria agent capable of prevention ruminant oxalism and preparation method and application |
| CN106544299B (en) * | 2016-11-04 | 2019-07-12 | 中北大学 | A kind of composite bacteria agent and the preparation method and application thereof preventing ruminant oxalism |
| CN106820155A (en) * | 2016-12-30 | 2017-06-13 | 江西益盟科技有限公司 | Lactic bacteria composition for regulating intestinal canal flora and strengthen immunity and preparation method thereof |
| CN107136322A (en) * | 2017-07-03 | 2017-09-08 | 青岛玛斯特生物技术有限公司 | Special-purpose protecting agent and its application are sprayed after a kind of aquatic feeds |
| CN107136322B (en) * | 2017-07-03 | 2018-06-29 | 青岛玛斯特生物技术有限公司 | Special-purpose protecting agent and its application are sprayed after a kind of aquatic feeds |
| CN109717481A (en) * | 2018-12-27 | 2019-05-07 | 广州智特奇生物科技股份有限公司 | A kind of preparation process of coating probiotics |
| CN109717481B (en) * | 2018-12-27 | 2022-04-26 | 广州智特奇生物科技股份有限公司 | Preparation process of coated probiotics |
| CN110521857A (en) * | 2019-08-19 | 2019-12-03 | 南京师盛生态环境研究院有限公司 | A kind of preparation method of high density resistance lactobacillus micro-capsule |
| CN112244153A (en) * | 2020-08-29 | 2021-01-22 | 广州大北农农牧科技有限责任公司 | Preparation method of double-layer coated lactic acid bacteria microcapsule |
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Application publication date: 20150617 |