CN104397351A - Biological antimicrobial-free feed additive and preparation method thereof - Google Patents
Biological antimicrobial-free feed additive and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a biological antimicrobial-free feed additive and a preparation method thereof, and belongs to the field of animal food. The biological antimicrobial-free feed additive mainly comprises the following components in parts by weight: 28-35 parts of yeast cell wall polysaccharide, 23-30 parts of bacillus licheniformis, 28-45 parts of bacillus subtilis, 17-19 parts of lactobacillus acidophilus, 17-19 parts of clostridium butyricum, 5-10 parts of selenium yeast, 60-70 parts of a coated slow-release acidifying agent, 150-160 parts of wheat bran, 120-150 parts of stone powder and a complex enzyme preparation, wherein the complex enzyme preparation comprises the following raw materials in parts by weight: 7-10 parts of phytase, 7-10 parts of glucanase, 10-15 parts of zytase and 7-10 parts of cellulase. The feed additive provided by the invention can produce various nutrient substances such as beneficial bacteria, complex enzyme and yeast peptides as being subjected to three fermentation processes of liquid tertiary fermentation, solid fermentation and biological feed fermentation, promotes the growth of intestinal villi, enhances the disease-resistant and disease-preventing abilities, and is mainly used for biological anti-free feed.
Description
Technical field
The invention belongs to field of animal feed, specifically, particularly relate to a kind of biological nonreactive feed addictive and preparation method thereof.
Background technology
20th century added antibiotics in feed, brought the develop rapidly of global livestock breeding industry.But the antibiotic feed of the feeding interpolation of Long term Animal, can produce antibody-resistant bacterium in vivo, and causes the problems such as the medicament residue of animal product.Along with the progressively raising of living standards of the people, consumer is transitioned into pursuit quality from pursuit quantity to the demand of animal products, more and more be restricted so add antibiotic way in feed, many countries in area have forbidden adding antibiotic in feed in Europe, North America, Asia etc. at present.Add antibiotic meeting heavy damage animal and bird intestines microbiota, reduce autoimmunity and the anti-stress ability of livestock and poultry, livestock and poultry are made to be in sub-health state for a long time, to most epidemic disease irresistance, and antibiotic can destroy intestinal mucosa, reduce the digestive utilization ratio of feed, affect the speed of growth of livestock and poultry; Antibiotic can remain in a large number in the meat of livestock and poultry, egg product and internal organs, also can be excreted in natural environment along with fecaluria, finally enter human body by food chain, and harm humans is healthy; Antibiotic abuse, causes the antibody-resistant bacterium in environment to produce in a large number, has a strong impact on the curative effect using antibiotic therapy animals and humans epidemic disease.
Summary of the invention
The object of the invention is to overcome above-mentioned defect, a kind of biological nonreactive feed addictive and preparation method thereof is provided, this feed addictive produces the multiple nutrients materials such as beneficial bacterium, complex enzyme, yeast polypeptides through three kinds of zymotechniques of liquid three grade fermemtation, solid fermentation and biological feedstuff, promote intestinal villi growth, strengthen defending and fighting against diseases ability.
Described biological nonreactive feed addictive, comprise the raw material components of following weight portion: yeast cell wall polysaccharide 28-35 part, bacillus licheniformis 23-30 part, bacillus subtilis 28-45 part, lactobacillus acidophilus 17-19 part, clostridium butyricum 17-19 part, yeast selenium 5-10 part, coated slow-release acidulant 60-70 part, wheat bran 150-160 part, stone flour 120-150 part and complex enzyme formulation, described complex enzyme formulation comprises phytase 7-10 part, dextranase 7-10 part, zytase 10-15 part, cellulase 7-10 part.
Described biological nonreactive feed addictive, comprises the raw material components of following preferred weight part: yeast cell wall polysaccharide 30 parts, bacillus licheniformis 25 parts, bacillus subtilis 40 parts, lactobacillus acidophilus 17.5 parts, clostridium butyricum 17.5 parts, yeast selenium 5 parts, coated slow-release acidulant 60 parts, phytase 7.5 parts, dextranase 7.5 parts, zytase 10 parts, cellulase 7.5 parts, 150 parts, wheat bran, stone flour 125 parts.
The preparation method of described biological nonreactive feed addictive, comprises the following steps:
One) multiple probio composite fermentation material is prepared:
(1) microorganism fungus kind is prepared: get bacillus licheniformis, bacillus subtilis, lactobacillus acidophilus, clostridium butyricum as the bacterium that sets out, respectively through domestication, choosing carry, rejuvenation, obtain the object bacterial classification that can reach resistance to pelleting temperature less than 80 DEG C, then the obtained bacteria containing amount that spreads cultivation respectively is 10,000,000,000/ml---the various bacterium liquid of 20,000,000,000/ml, namely obtain bacillus licheniformis kind liquid, bacillus subtilis bacterial classification liquid, clostridium butyricum kind liquid, lactobacillus acidophilus kind liquid.
1) liquid bacillus licheniformis, bacillus subtilis, clostridium butyricum strain liquid fermentation production process:
Test tube slant bacterial classification through expansion numerous after access eggplant type culture in glassware, 100L first class seed pot is accessed after being cultured to logarithmic phase, 1000L secondary seed tank is accessed again after spreading cultivation, after secondary seed tank is cultured to logarithmic phase, accesses 15000L fermentation tank culture puts tank to during gemma maturing rate more than 95%, namely obtain bacillus licheniformis kind liquid, bacillus subtilis, clostridium butyricum kind liquid.
2) liquid acidophilic's lactobacillus strain liquid fermentation production process:
Test tube slant bacterial classification through expansion numerous after access triangle and shake culture in glassware, 100L first class seed pot is accessed after being cultured to logarithmic phase, 1000L secondary seed tank is accessed again after spreading cultivation, after secondary seed tank is cultured to logarithmic phase, accesses 15000L fermentation tank constant temperature culture puts tank after 45-50 hours, namely obtain lactobacillus acidophilus kind liquid.
(2) the single bacterium fermentation material of microorganism is made:
1) the lichen bacillus ferments material is made
Make the lichen bacillus ferments culture medium: the raw material getting following weight: corn flour: 10-20 part, wheat flour: 5-10 part, wheat bran: 15-30 part, bean cake powder: 8-20 part, Cottonseed Meal powder: 3-8 part, corn germ cake: 8-20 part, yeast extract powder: 1-3 part, corn steep liquor containing 70-90% moisture content: 2-5 part, the molasses containing 10-60% moisture content: 2-8 part; The raw material obtained is added water, stirred, and raw material and the ratio 70-60:30-40 of the weight of the water added, obtain compound material, compound material put into high-pressure steam sterilizing pan, sterilizing 30-35 minutes under 0.1-0.12Mpa air pressure; Then taken the dish out of the pot by compound material, material temperature degree to be composite is down to less than 55 DEG C in an aseptic environment, obtains the lichen bacillus ferments culture medium;
Obtained bacillus licheniformis kind liquid is added in the lichen bacillus ferments culture medium and inoculates, the bacillus licheniformis kind liquid wherein added is 1-3:97-99 with the ratio of the proportion of the lichen bacillus ferments culture medium, be cultivate 2-3 days under the oxygen environment of 35 DEG C-37 DEG C in temperature, treat that viable count is greater than 1x108/gram, when xylanase activity is greater than 1.2X108IU/g, water content <15% is dried to again under the environment of temperature less than 55 DEG C, then pack, keep in Dark Place, obtain the lichen bacillus ferments material;
2) fermentation of bacillus subtilis material is made
Make fermentation of bacillus subtilis culture medium: the raw material getting following weight: corn flour: 10-20 part, wheat flour: 5-10 part, wheat bran: 15-30 part, bean cake powder: 8-20 part, Cottonseed Meal powder: 3-8 part, corn germ cake: 8-20 part, yeast extract powder: 1-3 part, corn steep liquor containing 70-90% moisture content: 2-5 part, the molasses containing 10-60% moisture content: 2-8 part; The raw material obtained is added water, stirred, and raw material and the ratio 70-60:30-40 of the weight of the water added, obtain compound material, compound material put into high-pressure steam sterilizing pan, sterilizing 30-35 minutes under 0.1-0.12Mpa air pressure; Then taken the dish out of the pot by compound material, material temperature degree to be composite is down to less than 55 DEG C in an aseptic environment, obtains fermentation of bacillus subtilis culture medium;
Obtained bacillus subtilis bacterial classification liquid is added in fermentation of bacillus subtilis culture medium and inoculates, the bacillus subtilis bacterial classification liquid wherein added is 1-3:97-99 with the ratio of the proportion of fermentation of bacillus subtilis culture medium, be cultivate 2-3 days under the oxygen environment of 35 DEG C-37 DEG C in temperature, treat that viable count is greater than 1x108/gram, when xylanase activity is greater than 1.2X108IU/g, water content <15% is dried to again under the environment of temperature less than 55 DEG C, then pack, keep in Dark Place, obtain fermentation of bacillus subtilis material;
3) lactobacillus acidophilus fermentation material is made
Make lactobacillus acidophilus culture medium: the raw material getting following weight: corn flour: 10-20 part, wheat flour: 5-10 part, wheat bran: 20-40 part, bean cake powder: 3-8 part, Cottonseed Meal powder: 10-20 part, corn germ cake: 17-27 part, corn steep liquor containing 70-90% moisture content: 2-5 part, molasses containing 10-60% moisture content: 2-8 part, the raw material obtained is added water, stir, the ratio 70-60:30-40 of raw material and the weight of the water added, obtain compound material, compound material is put into high-pressure steam sterilizing pan, sterilizing 30-35 minutes under 0.l-0.12Mpa air pressure, then taken the dish out of the pot by compound material, material temperature degree to be composite is down to less than 55 DEG C in an aseptic environment, obtains fermentation by saccharomyces cerevisiae culture medium,
Obtained lactobacillus acidophilus kind liquid is added in lactobacillus acidophilus fermentation medium and inoculates, the lactobacillus acidophilus kind liquid wherein added is 1-3:97-99 with the ratio of the proportion of lactobacillus acidophilus fermentation medium, be cultivate 2-3 days under the oxygen environment of 35 DEG C-42 DEG C in temperature, when viable count is greater than 1x108/gram, water content <15% is dried to again under the environment of temperature less than 55 DEG C, then pack, keep in Dark Place, obtain lactobacillus acidophilus fermentation material;
4) clostridium butyricum fermentation material is made
Make clostridium butyricum culture medium: the raw material getting following weight: corn flour: 10-20 part, wheat flour: 8-10 part, wheat bran: 15-40 part, bean cake powder: 3-9 part, Cottonseed Meal powder: 10-20 part, corn germ cake: 15-25 part, corn steep liquor containing 70-90% moisture content: 2-5 part, molasses containing 10-60% moisture content: 1-8 part, the raw material obtained is added water, stir, the ratio 70-60:30-40 of raw material and the weight of the water added, obtain compound material, compound material is put into high-pressure steam sterilizing pan, sterilizing 30-35 minutes under 0.l-0.12Mpa air pressure, then taken the dish out of the pot by compound material, material temperature degree to be composite is down to less than 55 DEG C in an aseptic environment, obtains fermentation by saccharomyces cerevisiae culture medium,
Obtained clostridium butyricum kind liquid is added in clostridium butyricum fermentation medium and inoculates, the clostridium butyricum kind liquid wherein added is 1-3:97-99 with the ratio of the proportion of clostridium butyricum fermentation medium, be cultivate 2-3 days under the oxygen environment of 35 DEG C-42 DEG C in temperature, when viable count is greater than 1x108/gram, water content <15% is dried to again under the environment of temperature less than 55 DEG C, then pack, keep in Dark Place, obtain clostridium butyricum fermentation material;
(2) compound microorganism ferments material is configured: by above-mentioned each fermentation material by after following parts by weight proportioning, through fully stirring, mixing; Obtained composite fermentation material, its proportioning is: the lichen bacillus ferments material: 6-10 part, fermentation of bacillus subtilis material: 6-10 part, lactobacillus acidophilus fermentation material: 5-8 part, clostridium butyricum fermentation material: 5-8 part;
(3) antibiotic-free mixed feedstuff is configured: parts by weight mix and blend complex enzyme formulation and multiple probio composite fermentation material being pressed 2-8:98-92, obtained biological nonreactive feed addictive.
The invention has the beneficial effects as follows:
1, the present invention is through three kinds of zymotechniques of liquid three grade fermemtation, solid fermentation and biological feedstuff, produces the multiple nutrients materials such as beneficial bacterium, complex enzyme, yeast polypeptides, promotes intestinal villi growth, strengthens defending and fighting against diseases ability; The nutrient digestion be conducive in feed absorbs and utilizes, the defending and fighting against diseases ability of improving food conversion ratio, reducing feed cost, strengthening animal.Improve poultry house environment, adjustment micro ecology of gastrointestinal tract balance, the generation reducing the generation of enterobacterial diseases, effectively control and reduce breathing problem, the survival rate reducing the death rate, improve livestock and poultry;
2, the present invention's biological nonreactive feed addictive beneficial bacterium is wide in variety, quantity is large, simultaneously also containing multiple high activity digestive ferments such as dextranase, zytase, cellulase, protease, serve good synergy, animal body immunity can be improved, improve intestine microenvironment, thus improve animal health condition, and improve feed digestion utilization rate, promote fanning economics and meat, Egg Quality;
3, the biological nonreactive feed addictive of the present invention can effectively improve animal body premunition, resisting stress, scavenging free radicals, the diseases such as prevention aftosa, hyperpyrexia disease, swine fever, bird flu, improve efficiency of feed utilization 8 ~ 10%, promote growth of animal, reduce and draw raw material 5 ~ 10%, reduce ight soil 10%; And effectively improve bird draining just undesirable condition, reduce the generation of piglet white scour;
4, edible feed addictive of the present invention, laying hen eggshell color obviously improves, and percentage of damage reduces, and peak period extends, and eliminates chicken feather serviceability rate high, watery diarrhea, and the diarrhoea that especially high heat stress causes obviously reduces; Broiler chicken effectively controls the incidence of the diseases such as enteric bacteria, yellow water sample excrement, fish intestines sample excrement obviously reduce, and chicken group situation is good, and regularity is high, mortality declines, and chicken circle is dry, feather light, toe yellow hat worn by a Taoist priest is red, and drug costs significantly declines, and every chicken drug costs reduces 0.2-0.3 unit; Diarrhea of pigs obviously reduces, and does not digest particle and obviously reduce in ight soil, and parturient sow constipation obviously alleviates;
The principle of the Element release far infrareds such as the alkali ion pumping source 5, utilizing the strong base-weak acid salt such as carbonate, metasilicate to be formed and Ge, Si, body can be made to keep alkalescent body fluid, develop immunitypty and pharmaceutically active, synergistic effect can be played to vaccine, medicine and suppress the effect of virus, without incompatibility.Antistress function, effectively can slow down the stress reaction because group of reloading, turn, disconnected beak, vaccine inoculation etc. cause, especially resist heat stress.
Detailed description of the invention
Embodiment one: described biological nonreactive feed addictive, comprise the raw material components of following weight portion: yeast cell wall polysaccharide 30 parts, bacillus licheniformis (more than 20,000,000,000 units) 25 parts, bacillus subtilis (more than 20,000,000,000 units) 40 parts, lactobacillus acidophilus (more than 20,000,000,000 units) 17.5 parts, clostridium butyricum 17.5 parts, yeast selenium 5 parts, coated slow-release acidulant 60 parts, 150 parts, wheat bran, stone flour 125 parts and complex enzyme formulation, described complex enzyme formulation comprises phytase 7.5 parts, dextranase 7.5 parts, zytase 10 parts, cellulase 7.5 parts.
The adding proportion of additive of the present invention generally in perfect compound feed is 1% ~ 2%; In concentrate feed, adding proportion is 15%-28%.
Embodiment two: described biological nonreactive feed addictive, comprises the raw material components of following weight portion: yeast cell wall polysaccharide 28 parts, bacillus licheniformis 23 parts, bacillus subtilis 28 parts, lactobacillus acidophilus 17 parts, clostridium butyricum 17 parts, yeast selenium 5 parts, coated slow-release acidulant 60 parts, 150 parts, wheat bran, stone flour 120 parts and complex enzyme formulation, described complex enzyme formulation comprises phytase 7 parts, dextranase 7 parts, zytase 10 parts, cellulase 7 parts.
Embodiment three: described biological nonreactive feed addictive, comprises the raw material components of following weight portion: yeast cell wall polysaccharide 35 parts, bacillus licheniformis 30 parts, bacillus subtilis 45 parts, lactobacillus acidophilus 19 parts, clostridium butyricum 19 parts, yeast selenium 10 parts, coated slow-release acidulant 70 parts, 160 parts, wheat bran, stone flour 150 parts and complex enzyme formulation, described complex enzyme formulation comprises phytase 10 parts, dextranase 10 parts, zytase 15 parts, cellulase 10 parts.
The preparation method of described biological nonreactive feed addictive, comprises the following steps:
One) multiple probio composite fermentation material is prepared:
(1) microorganism fungus kind is prepared: get bacillus licheniformis, bacillus subtilis, lactobacillus acidophilus, clostridium butyricum as the bacterium that sets out, respectively through domestication, choosing carry, rejuvenation, obtain the object bacterial classification that can reach resistance to pelleting temperature less than 80 DEG C, then the obtained bacteria containing amount that spreads cultivation respectively is 10,000,000,000/ml---the various bacterium liquid of 20,000,000,000/ml, namely obtain bacillus licheniformis kind liquid, bacillus subtilis bacterial classification liquid, clostridium butyricum kind liquid, lactobacillus acidophilus kind liquid.
1) liquid bacillus licheniformis, bacillus subtilis, clostridium butyricum strain liquid fermentation production process:
Test tube slant bacterial classification through expansion numerous after access eggplant type culture in glassware, 100L first class seed pot is accessed after being cultured to logarithmic phase, 1000L secondary seed tank is accessed again after spreading cultivation, after secondary seed tank is cultured to logarithmic phase, accesses 15000L fermentation tank culture puts tank to during gemma maturing rate more than 95%, namely obtain bacillus licheniformis kind liquid, bacillus subtilis, clostridium butyricum kind liquid.
2) liquid acidophilic's lactobacillus strain liquid fermentation production process:
Test tube slant bacterial classification through expansion numerous after access triangle and shake culture in glassware, 100L first class seed pot is accessed after being cultured to logarithmic phase, 1000L secondary seed tank is accessed again after spreading cultivation, after secondary seed tank is cultured to logarithmic phase, accesses 15000L fermentation tank constant temperature culture puts tank after 48 hours, namely obtain lactobacillus acidophilus kind liquid.
(2) the single bacterium fermentation material of microorganism is made:
1) the lichen bacillus ferments material is made
Make the lichen bacillus ferments culture medium: the raw material getting following weight: corn flour: 15 parts, wheat flour: 7 parts, wheat bran: 18 parts, bean cake powder: 12 parts, Cottonseed Meal powder: 5 parts, corn germ cake: 14 parts, yeast extract powder: 2 parts, corn steep liquor containing 70-90% moisture content: 2 parts, the molasses containing 10-60% moisture content: 3 parts; The raw material obtained is added water, stirred, and raw material and the ratio 79:21 of the weight of the water added, obtain compound material, compound material put into high-pressure steam sterilizing pan, sterilizing 30 minutes under 0.1-0.12Mpa air pressure; Then taken the dish out of the pot by compound material, material temperature degree to be composite is down to less than 55 DEG C in an aseptic environment, obtains the lichen bacillus ferments culture medium;
Obtained bacillus licheniformis kind liquid is added in the lichen bacillus ferments culture medium and inoculates, the bacillus licheniformis kind liquid wherein added is 2:98 with the ratio of the proportion of the lichen bacillus ferments culture medium, be cultivate 2-3 days under the oxygen environment of 35 DEG C-37 DEG C in temperature, treat that viable count is greater than 1x10
8individual/gram, xylanase activity is greater than 1.2X10
8during IU/g, then be dried to water content <15% under the environment of temperature less than 55 DEG C, then pack, keep in Dark Place, obtain the lichen bacillus ferments material;
2) fermentation of bacillus subtilis material is made
Make fermentation of bacillus subtilis culture medium: the raw material getting following weight: corn flour: 15 parts, wheat flour: 7 parts, wheat bran: 18 parts, bean cake powder: 12 parts, Cottonseed Meal powder: 5 parts, corn germ cake: 14 parts, yeast extract powder: 2 parts, corn steep liquor containing 70-90% moisture content: 3 parts, the molasses containing 10-60% moisture content: 3 parts; The raw material obtained is added water, stirred, and raw material and the ratio 79:21 of the weight of the water added, obtain compound material, compound material put into high-pressure steam sterilizing pan, sterilizing 30 minutes under 0.1-0.12Mpa air pressure; Then taken the dish out of the pot by compound material, material temperature degree to be composite is down to less than 55 DEG C in an aseptic environment, obtains fermentation of bacillus subtilis culture medium;
Obtained bacillus subtilis bacterial classification liquid is added in fermentation of bacillus subtilis culture medium and inoculates, the bacillus subtilis bacterial classification liquid wherein added is 2:98 with the ratio of the proportion of fermentation of bacillus subtilis culture medium, be cultivate 2-3 days under the oxygen environment of 35 DEG C-37 DEG C in temperature, treat that viable count is greater than 1x10
8individual/gram, xylanase activity is greater than.1.2x10
8during IU/g, then be dried to water content <15% under the environment of temperature less than 55 DEG C, then pack, keep in Dark Place, obtain fermentation of bacillus subtilis material;
3) lactobacillus acidophilus fermentation material is made
Make lactobacillus acidophilus culture medium: the raw material getting following weight: corn flour: 15 parts, wheat flour: 10 parts, wheat bran: 20 parts, bean cake powder: 8 parts, Cottonseed Meal powder: 10 parts, corn germ cake: 17 parts, corn steep liquor containing 70-90% moisture content: 3 parts, molasses containing 10-60% moisture content: 7 parts, the raw material obtained is added water, stirred, and raw material and the ratio 80:20 of the weight of the water added, obtain compound material, compound material is put into high-pressure steam sterilizing pan, sterilizing 30 minutes under 0.l-0.12Mpa air pressure; Then taken the dish out of the pot by compound material, material temperature degree to be composite is down to less than 55 DEG C in an aseptic environment, obtains fermentation by saccharomyces cerevisiae culture medium;
Obtained lactobacillus acidophilus kind liquid is added in lactobacillus acidophilus fermentation medium and inoculates, the lactobacillus acidophilus kind liquid wherein added is 3:97 with the ratio of the proportion of lactobacillus acidophilus fermentation medium, be cultivate 2-3 days under the oxygen environment of 35 DEG C-42 DEG C in temperature, treat that viable count is greater than 1x10
8individual/gram time, then be dried to water content <15% under the environment of temperature less than 55 DEG C, then pack, keep in Dark Place, obtain lactobacillus acidophilus fermentation material;
4) clostridium butyricum fermentation material is made
Make clostridium butyricum culture medium: the raw material getting following weight: corn flour: 10-20 part, wheat flour: 8-10 part, wheat bran: 15-40 part, bean cake powder: 3-9 part, Cottonseed Meal powder: 10-20 part, corn germ cake: 15-25 part, corn steep liquor containing 70-90% moisture content: 2-5 part, molasses containing 10-60% moisture content: 1-8 part, the raw material obtained is added water, stir, the ratio 70-60:30-40 of raw material and the weight of the water added, obtain compound material, compound material is put into high-pressure steam sterilizing pan, sterilizing 30-35 minutes under 0.l-0.12Mpa air pressure, then taken the dish out of the pot by compound material, material temperature degree to be composite is down to less than 55 DEG C in an aseptic environment, obtains fermentation by saccharomyces cerevisiae culture medium,
Obtained clostridium butyricum kind liquid is added in clostridium butyricum fermentation medium and inoculates, the clostridium butyricum kind liquid wherein added is 1-3:97-99 with the ratio of the proportion of clostridium butyricum fermentation medium, be cultivate 2-3 days under the oxygen environment of 35 DEG C-42 DEG C in temperature, when viable count is greater than 1x108/gram, water content <15% is dried to again under the environment of temperature less than 55 DEG C, then pack, keep in Dark Place, obtain clostridium butyricum fermentation material;
(2) compound microorganism ferments material is configured: by above-mentioned each fermentation material by after following parts by weight proportioning, through fully stirring, mixing; Obtained composite fermentation material, its proportioning is: the lichen bacillus ferments material: 8 parts, fermentation of bacillus subtilis material: 8 parts, lactobacillus acidophilus fermentation material: 5 parts, clostridium butyricum fermentation material: 5 parts;
(3) antibiotic-free mixed feedstuff is configured: parts by weight mix and blend complex enzyme formulation and multiple probio composite fermentation material being pressed 5:95, obtained biological nonreactive feed addictive.
Claims (3)
1. a biological nonreactive feed addictive, it is characterized in that: the raw material components comprising following weight portion: yeast cell wall polysaccharide 28-35 part, bacillus licheniformis 23-30 part, bacillus subtilis 28-45 part, lactobacillus acidophilus 17-19 part, clostridium butyricum 17-19 part, yeast selenium 5-10 part, coated slow-release acidulant 60-70 part, wheat bran 150-160 part, stone flour 120-150 part and complex enzyme formulation, described complex enzyme formulation comprises phytase 7-10 part, dextranase 7-10 part, zytase 10-15 part, cellulase 7-10 part.
2. biological nonreactive feed addictive according to claim 1, is characterized in that: the raw material components comprising following weight portion: yeast cell wall polysaccharide 30 parts, bacillus licheniformis 25 parts, bacillus subtilis 40 parts, lactobacillus acidophilus 17.5 parts, clostridium butyricum 17.5 parts, yeast selenium 5 parts, coated slow-release acidulant 60 parts, phytase 7.5 parts, dextranase 7.5 parts, zytase 10 parts, cellulase 7.5 parts, 150 parts, wheat bran, stone flour 125 parts.
3. a preparation method for biological nonreactive feed addictive according to claim 1, is characterized in that: comprise the following steps:
One) multiple probio composite fermentation material is prepared:
(1) microorganism fungus kind is prepared: get bacillus licheniformis, bacillus subtilis, lactobacillus acidophilus, clostridium butyricum as the bacterium that sets out, respectively through domestication, choosing carry, rejuvenation, obtain the object bacterial classification that can reach resistance to pelleting temperature less than 80 DEG C, then the obtained bacteria containing amount that spreads cultivation respectively is 10,000,000,000/ml---the various bacterium liquid of 20,000,000,000/ml, namely obtain bacillus licheniformis kind liquid, bacillus subtilis bacterial classification liquid, clostridium butyricum kind liquid, lactobacillus acidophilus kind liquid.
1) liquid bacillus licheniformis, bacillus subtilis, clostridium butyricum strain liquid fermentation production process:
Test tube slant bacterial classification through expansion numerous after access eggplant type culture in glassware, 100L first class seed pot is accessed after being cultured to logarithmic phase, 1000L secondary seed tank is accessed again after spreading cultivation, after secondary seed tank is cultured to logarithmic phase, accesses 15000L fermentation tank culture puts tank to during gemma maturing rate more than 95%, namely obtain bacillus licheniformis kind liquid, bacillus subtilis, clostridium butyricum kind liquid.
2) liquid acidophilic's lactobacillus strain liquid fermentation production process:
Test tube slant bacterial classification through expansion numerous after access triangle and shake culture in glassware, 100L first class seed pot is accessed after being cultured to logarithmic phase, 1000L secondary seed tank is accessed again after spreading cultivation, after secondary seed tank is cultured to logarithmic phase, accesses 15000L fermentation tank constant temperature culture puts tank after 45-50 hours, namely obtain lactobacillus acidophilus kind liquid.
(2) the single bacterium fermentation material of microorganism is made:
1) the lichen bacillus ferments material is made
Make the lichen bacillus ferments culture medium: the raw material getting following weight: corn flour: 10-20 part, wheat flour: 5-10 part, wheat bran: 15-30 part, bean cake powder: 8-20 part, Cottonseed Meal powder: 3-8 part, corn germ cake: 8-20 part, yeast extract powder: 1-3 part, corn steep liquor containing 70-90% moisture content: 2-5 part, the molasses containing 10-60% moisture content: 2-8 part; The raw material obtained is added water, stirred, and raw material and the ratio 70-80:30-20 of the weight of the water added, obtain compound material, compound material put into high-pressure steam sterilizing pan, sterilizing 30-35 minutes under 0.1-0.12Mpa air pressure; Then taken the dish out of the pot by compound material, material temperature degree to be composite is down to less than 55 DEG C in an aseptic environment, obtains the lichen bacillus ferments culture medium;
Obtained bacillus licheniformis kind liquid is added in the lichen bacillus ferments culture medium and inoculates, the bacillus licheniformis kind liquid wherein added is 1-3:97-99 with the ratio of the proportion of the lichen bacillus ferments culture medium, be cultivate 2-3 days under the oxygen environment of 35 DEG C-37 DEG C in temperature, treat that viable count is greater than 1x10
8individual/gram, xylanase activity is greater than 1.2X 10
8during IU/g, then be dried to water content <15% under the environment of temperature less than 55 DEG C, then pack, keep in Dark Place, obtain the lichen bacillus ferments material;
2) fermentation of bacillus subtilis material is made
Make fermentation of bacillus subtilis culture medium: the raw material getting following weight: corn flour: 10-20 part, wheat flour: 5-10 part, wheat bran: 15-30 part, bean cake powder: 8-20 part, Cottonseed Meal powder: 3-8 part, corn germ cake: 8-20 part, yeast extract powder: 1-3 part, corn steep liquor containing 70-90% moisture content: 2-5 part, the molasses containing 10-60% moisture content: 2-8 part; The raw material obtained is added water, stirred, and raw material and the ratio 70-80:30-20 of the weight of the water added, obtain compound material, compound material put into high-pressure steam sterilizing pan, sterilizing 30-35 minutes under 0.1-0.12Mpa air pressure; Then taken the dish out of the pot by compound material, material temperature degree to be composite is down to less than 55 DEG C in an aseptic environment, obtains fermentation of bacillus subtilis culture medium;
Obtained bacillus subtilis bacterial classification liquid is added in fermentation of bacillus subtilis culture medium and inoculates, the bacillus subtilis bacterial classification liquid wherein added is 1-3:97-99 with the ratio of the proportion of fermentation of bacillus subtilis culture medium, be cultivate 2-3 days under the oxygen environment of 35 DEG C-37 DEG C in temperature, treat that viable count is greater than 1x108/gram, when xylanase activity is greater than 1.2X 108IU/g, water content <15% is dried to again under the environment of temperature less than 55 DEG C, then pack, keep in Dark Place, obtain fermentation of bacillus subtilis material;
3) lactobacillus acidophilus fermentation material is made
Make lactobacillus acidophilus culture medium: the raw material getting following weight: corn flour: 10-20 part, wheat flour: 5-10 part, wheat bran: 20-40 part, bean cake powder: 3-8 part, Cottonseed Meal powder: 10-20 part, corn germ cake: 17-27 part, corn steep liquor containing 70-90% moisture content: 2-5 part, molasses containing 10-60% moisture content: 2-8 part, the raw material obtained is added water, stir, the ratio 70-60:30-40 of raw material and the weight of the water added, obtain compound material, compound material is put into high-pressure steam sterilizing pan, sterilizing 30-35 minutes under 0.l-0.12Mpa air pressure, then taken the dish out of the pot by compound material, material temperature degree to be composite is down to less than 55 DEG C in an aseptic environment, obtains fermentation by saccharomyces cerevisiae culture medium,
Obtained lactobacillus acidophilus kind liquid is added in lactobacillus acidophilus fermentation medium and inoculates, the lactobacillus acidophilus kind liquid wherein added is 1-3:97-99 with the ratio of the proportion of lactobacillus acidophilus fermentation medium, be cultivate 2-3 days under the oxygen environment of 35 DEG C-42 DEG C in temperature, when viable count is greater than 1x108/gram, water content <15% is dried to again under the environment of temperature less than 55 DEG C, then pack, keep in Dark Place, obtain lactobacillus acidophilus fermentation material;
4) clostridium butyricum fermentation material is made
Make clostridium butyricum culture medium: the raw material getting following weight: corn flour: 10-20 part, wheat flour: 8-10 part, wheat bran: 15-40 part, bean cake powder: 3-9 part, Cottonseed Meal powder: 10-20 part, corn germ cake: 15-25 part, corn steep liquor containing 70-90% moisture content: 2-5 part, molasses containing 10-60% moisture content: 1-8 part, the raw material obtained is added water, stir, the ratio 70-80:30-20 of raw material and the weight of the water added, obtain compound material, compound material is put into high-pressure steam sterilizing pan, sterilizing 30-35 minutes under 0.l-0.12Mpa air pressure, then taken the dish out of the pot by compound material, material temperature degree to be composite is down to less than 55 DEG C in an aseptic environment, obtains fermentation by saccharomyces cerevisiae culture medium,
Obtained clostridium butyricum kind liquid is added in clostridium butyricum fermentation medium and inoculates, the clostridium butyricum kind liquid wherein added is 1-3:97-99 with the ratio of the proportion of clostridium butyricum fermentation medium, be cultivate 2-3 days under the oxygen environment of 35 DEG C-42 DEG C in temperature, when viable count is greater than 1x108/gram, water content <15% is dried to again under the environment of temperature less than 55 DEG C, then pack, keep in Dark Place, obtain clostridium butyricum fermentation material;
(2) compound microorganism ferments material is configured: by above-mentioned each fermentation material by after following parts by weight proportioning, through fully stirring, mixing; Obtained composite fermentation material, its proportioning is: the lichen bacillus ferments material: 6-10 part, fermentation of bacillus subtilis material: 6-10 part, lactobacillus acidophilus fermentation material: 5-8 part, clostridium butyricum fermentation material: 5-8 part;
(3) antibiotic-free mixed feedstuff is configured: parts by weight mix and blend complex enzyme formulation and multiple probio composite fermentation material being pressed 2-8:98-92, obtained biological nonreactive feed addictive.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101828634A (en) * | 2010-04-16 | 2010-09-15 | 湖南创新生物科技有限公司 | Microbial fermentation antibiotic-free feed and preparing method thereof |
| CN102318735A (en) * | 2011-09-23 | 2012-01-18 | 济宁市天瑞通科技开发有限公司 | Antibiotic-free mixed feedstuff and preparation method thereof |
| CN103005210A (en) * | 2012-12-25 | 2013-04-03 | 广州旺大饲料科技有限公司 | Pig feed additive capable of replacing antibiotics, and preparation method and application thereof |
| CN104012796A (en) * | 2014-05-26 | 2014-09-03 | 山东龙海生物科技有限公司 | Biological non-antibiotic complete feed for piglet and preparation method thereof |
-
2014
- 2014-11-24 CN CN201410681227.7A patent/CN104397351A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101828634A (en) * | 2010-04-16 | 2010-09-15 | 湖南创新生物科技有限公司 | Microbial fermentation antibiotic-free feed and preparing method thereof |
| CN102318735A (en) * | 2011-09-23 | 2012-01-18 | 济宁市天瑞通科技开发有限公司 | Antibiotic-free mixed feedstuff and preparation method thereof |
| CN103005210A (en) * | 2012-12-25 | 2013-04-03 | 广州旺大饲料科技有限公司 | Pig feed additive capable of replacing antibiotics, and preparation method and application thereof |
| CN104012796A (en) * | 2014-05-26 | 2014-09-03 | 山东龙海生物科技有限公司 | Biological non-antibiotic complete feed for piglet and preparation method thereof |
Cited By (23)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
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