CN104697822B - A kind of expiration sampling bottle available for SPME - Google Patents
A kind of expiration sampling bottle available for SPME Download PDFInfo
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- CN104697822B CN104697822B CN201510044964.0A CN201510044964A CN104697822B CN 104697822 B CN104697822 B CN 104697822B CN 201510044964 A CN201510044964 A CN 201510044964A CN 104697822 B CN104697822 B CN 104697822B
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- bottle
- vacuum valve
- spme
- outlet pipe
- admission line
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Abstract
The present invention relates to a kind of expiration sampling bottle available for SPME, including admission line, the first vacuum valve, bottle, outlet pipe, the second vacuum valve and SPME connectivity port.Admission line is located at bottle one end, and the first vacuum valve is used for sealing admission line；Outlet pipe is located at bottle other end, and the second vacuum valve is used for sealing outlet pipe；SPME connectivity port is located in the middle of bottle, and it mainly stays foraminate thread cap to constitute by threaded end (TE), dottle pin and centre.The expiration sampling bottle air-tightness is good, can be used to gather human exhaled breath, sampling process is convenient, and the main material of sampling bottle is glass, can reduce impurity interference, and can be combined with solid phase micro-extraction technique.
Description
Technical field
The invention belongs to analyze detection field, and in particular to a kind of expiration sampling bottle available for SPME.
Background technology
Containing many kinds of volatile organic matters (VOCs) in human exhaled breath, these materials reflect the metabolic mistake of human body
Journey and health status.The auxiliary diagnosis of disease is carried out by detecting the VOCs in human exhaled breath in recent years increasingly by weight
Depending on.Because the VOCs contents in expiration are very low, generally require to detect again after being enriched with VOCs with solid phase micro-extraction technique.
Traditional method is usually that first human exhaled breath is acquired with sampler bag, then SPME syringe needle is penetrated into sampler bag to exhaling
VOCs in gas is extracted, but sampler bag can also discharge some VOCs, thus can be to the testing result band of specimen of breath
To disturb.
The content of the invention
It is of the present invention to solve the problems, such as：There is provided a kind of glass for the impurity interference that may be brought in reduction expiration sampling extraction process
The expiration sampling bottle of the air-tightness of material well, the sampling bottle can be combined with solid phase micro-extraction technique.
The technical solution of the present invention：A kind of expiration sampling bottle available for SPME, including admission line,
One vacuum valve, bottle, outlet pipe, the second vacuum valve and SPME connectivity port.Admission line is located at bottle one
End, the first vacuum valve is used for sealing admission line, and the first vacuum valve is detachable, and its bonnet carries internal thread, is attached thereto
The glass mouth of pipe carry external screw thread, the opening and closing of admission line are controlled by rotating bonnet；Outlet pipe is located at bottle
Other end, the second vacuum valve is used for sealing outlet pipe, and the second vacuum valve can also be dismantled, the connection with the glass mouth of pipe
Mode is identical with the first vacuum valve, and the opening and closing of outlet pipe are controlled by rotating bonnet；SPME is connected
Port is located in the middle of bottle, and it mainly stays foraminate thread cap to constitute by threaded end (TE), dottle pin and centre.Admission line,
The material of bottle, outlet pipe and threaded end (TE) is glass, and the main material of the first vacuum valve and the second vacuum valve is poly-
Tetrafluoroethene, dottle pin uses pure silica gel material, is disturbed with reducing possible caused impurity.The length of threaded end (TE) is 1.4-
1.6 (can generally take 1.5cm), dottle pin is placed in in female lid, and aperture, thread cap and spiral shell are left in the middle of lid
The line mouth of pipe can tighten sealing.SPME syringe needle can be penetrated in bottle through dottle pin by aperture and extracted.
The use process of the present invention is as follows：Subject is by the disposable blow gun that is connected with admission line toward slowly blowing in bottle
Gas, is exhaled and is entered by admission line in expiration bottle, and original air is discharged from outlet pipe in bottle, blows several implications to ensure sky more
Gas is drained, and is finished in air blowing tighten immediately the first vacuum valve and the second vacuum valve, then the gas in the alveolar of expiration end out
Body is sealed in bottle.The expiration sampling bottle sealed after collection is taken back into laboratory and carries out breath analysis, can be by SPME
Syringe needle is penetrated in bottle through dottle pin by the aperture in the middle of lid and VOCs is extracted.
The advantage of the present invention compared with prior art is as follows：The container of glass material is cleaner, can avoid traditional sampling
The impurity interference that bag is brought；Bottle sealability of sampling is good, can prevent that specimen of breath from being polluted by outside air；Sampling process letter
It is single, and can be combined with solid phase micro-extraction technique.
Brief description of the drawings
Fig. 1 is integrally-built diagrammatic cross-section of the invention；
Fig. 2 is the diagrammatic cross-section of three components in SPME connectivity port in the present invention；
Fig. 3 for the present invention in will exhale sealing after with diagrammatic cross-section associated with solid phase micro-extraction technique.
Embodiment
As shown in Figure 1, 2, 3, a kind of expiration sampling bottle embodiment available for SPME of the invention includes：Air inlet
Pipeline 1, the first vacuum valve 2, bottle 3, outlet pipe 4, the second vacuum valve 5 and SPME connectivity port 6.Air inlet pipe
Road 1 is located at the one end of bottle 3, and the first vacuum valve 2 is used for sealing admission line 1, and the first vacuum valve 2 is detachable, its bonnet band
There is internal thread, the glass mouth of pipe being attached thereto carries external screw thread, and unlatching and the pass of admission line 1 are controlled by rotating bonnet
Close；Outlet pipe 4 is located at the other end of bottle 3, and the second vacuum valve is used for sealing outlet pipe 4, and the second vacuum valve 5 also may be used
It is identical with the connected mode and the first vacuum valve 2 of the glass mouth of pipe to dismantle, outlet pipe 4 is controlled by rotating bonnet
Open and close；SPME connectivity port 6 is located in the middle of bottle.As shown in Fig. 2 SPME connectivity port 6 is by spiral shell
The line mouth of pipe 7, dottle pin 8 and centre stay foraminate thread cap 9 to constitute.Stay placement in foraminate thread cap 9 in the centre
Foraminate thread cap 9 is stayed to tighten sealing, the micro- extraction of solid phase by internal thread and threaded end (TE) 7 in sizeable dottle pin 8, centre
Take syringe needle to penetrate in bottle 3 through dottle pin 8 by the aperture in the middle of lid to extract.Admission line 1, bottle 3, outlet pipe 4 and spiral shell
The line mouth of pipe 7 is constituted by columned glass tube, and wherein admission line 1 and outlet pipe 4 are all by two orthogonal glass
Glass pipe is formed by connecting, and the first vacuum valve 2 and the second vacuum valve 5 carry out air seal, valve and pipeline by sealing ring
The material of interior contact portion is polytetrafluoroethylene (PTFE), and dottle pin 8 uses pure silica gel material.Because the organic matter of such material is separated out
It is few, the pollution to expiration can be reduced.Admission line 1 can connect disposable blow gun, facilitate subject toward air blowing in bottle.Screwed pipe
The length about 1.5cm of mouth 7, its length is unsuitable oversize, because SPME needle length is certain, if threaded end (TE) is oversize, pin
Head will be difficult to reach in the middle of bottle 3, it is impossible to fully extraction.
The course of work of the present invention is：Subject is by the disposable blow gun that is connected with admission line 1 toward slowly blowing in bottle 3
Gas, exhales and is entered by admission line 1 in expiration bottle, original air is discharged from outlet pipe 4 in bottle, and air blowing is finished tightens immediately
First vacuum valve 2 and the second vacuum valve 5, the then gas come out in the alveolar of expiration end are sealed in bottle.After gathering
The expiration sampling bottle of sealing takes back laboratory and carries out breath analysis, as shown in figure 3, SPME syringe needle can be passed through into lid 9
Middle aperture is penetrated in bottle through dottle pin 8 and VOCs is extracted.
Above example is provided just for the sake of the description purpose of the present invention, and is not intended to limit the scope of the present invention.This
The scope of invention is defined by the following claims.The various equivalents that do not depart from spirit and principles of the present invention and make and repair
Change, all should cover within the scope of the present invention.
Claims (5)
1. a kind of expiration sampling bottle available for SPME, it is characterised in that including：Admission line (1), the first vacuum valve
Door (2), bottle (3), outlet pipe (4), the second vacuum valve (5) and SPME connectivity port (6)；Admission line (1)
Positioned at bottle (3) one end, the first vacuum valve (2) is used for sealing admission line (1), and the first vacuum valve (2) is detachable, its valve
Hatband has internal thread, and the glass mouth of pipe being attached thereto carries external screw thread, and the unlatching of admission line (1) is controlled by rotating bonnet
And closing；Outlet pipe (4) is located at bottle (3) other end, and the second vacuum valve (5) is used for sealing outlet pipe (4), second
Vacuum valve (5) can also be dismantled, identical with the connected mode and the first vacuum valve (2) of the glass mouth of pipe, by rotating bonnet
To control the opening and closing of outlet pipe (4)；SPME connectivity port (6) is located in the middle of bottle, and it is mainly by screw thread
The mouth of pipe (7), dottle pin (8) and centre stay foraminate thread cap (9) to constitute, and foraminate thread cap (9) is stayed in the centre
Interior to place sizeable dottle pin (8), centre stays foraminate thread cap (9) to be tightened by internal thread with threaded end (TE) (7)
Sealing, SPME syringe needle penetrates extraction in bottle (3) by the aperture in the middle of lid through dottle pin (8)；
The admission line (1), bottle (3), outlet pipe (4), threaded end (TE) (7) are constituted by columned glass tube, its
Middle admission line (1) and outlet pipe (4) are formed by connecting by two orthogonal glass tubes.
2. a kind of expiration sampling bottle available for SPME according to claim 1, it is characterised in that：The air inlet
Pipeline (1) is to exhale to enter passage, exhales and enters the disposable blow gun of passage upper end connection.
3. a kind of expiration sampling bottle available for SPME according to claim 1, it is characterised in that：First vacuum
Valve (2) and the second vacuum valve (5) carry out air seal, the material of valve and pipeline interior contact portion by sealing ring
For polytetrafluoroethylene (PTFE).
4. a kind of expiration sampling bottle available for SPME according to claim 1, it is characterised in that：The screw thread
The length of the mouth of pipe (7) is 1.4-1.6cm.
5. a kind of expiration sampling bottle available for SPME according to claim 1, it is characterised in that：The dottle pin
(8) pure silica gel material is used.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510044964.0A CN104697822B (en) | 2015-01-28 | 2015-01-28 | A kind of expiration sampling bottle available for SPME |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510044964.0A CN104697822B (en) | 2015-01-28 | 2015-01-28 | A kind of expiration sampling bottle available for SPME |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104697822A CN104697822A (en) | 2015-06-10 |
| CN104697822B true CN104697822B (en) | 2017-07-14 |
Family
ID=53345179
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510044964.0A Active CN104697822B (en) | 2015-01-28 | 2015-01-28 | A kind of expiration sampling bottle available for SPME |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104697822B (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10413216B2 (en) * | 2016-02-03 | 2019-09-17 | Quintron Instrument Company, Inc. | Breath testing apparatus |
| CN113447321A (en) * | 2021-08-17 | 2021-09-28 | 古海燕 | Oil refining chemical industry enterprise safety ring protects gaseous sampling device |
| CN114354736A (en) * | 2022-01-11 | 2022-04-15 | 深圳市步锐生物科技有限公司 | System and method for detecting metabolic difference foreign matters in exhaled air |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN202903554U (en) * | 2012-10-29 | 2013-04-24 | 中国石油大学(北京) | High-pressure gas sampling device |
| CN203122406U (en) * | 2013-01-11 | 2013-08-14 | 安徽养和医疗器械设备有限公司 | Exhaled gas sampling device |
| CN203849059U (en) * | 2014-05-08 | 2014-09-24 | 李海涛 | Device for collecting pollutants in air |
| CN203874645U (en) * | 2014-06-13 | 2014-10-15 | 国家电网公司 | Absorption bottle device for experimental tail gas in laboratory |
| CN104101518A (en) * | 2014-07-23 | 2014-10-15 | 安徽养和医疗器械设备有限公司 | Sampling bag for expiration tester |
-
2015
- 2015-01-28 CN CN201510044964.0A patent/CN104697822B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN202903554U (en) * | 2012-10-29 | 2013-04-24 | 中国石油大学(北京) | High-pressure gas sampling device |
| CN203122406U (en) * | 2013-01-11 | 2013-08-14 | 安徽养和医疗器械设备有限公司 | Exhaled gas sampling device |
| CN203849059U (en) * | 2014-05-08 | 2014-09-24 | 李海涛 | Device for collecting pollutants in air |
| CN203874645U (en) * | 2014-06-13 | 2014-10-15 | 国家电网公司 | Absorption bottle device for experimental tail gas in laboratory |
| CN104101518A (en) * | 2014-07-23 | 2014-10-15 | 安徽养和医疗器械设备有限公司 | Sampling bag for expiration tester |
Non-Patent Citations (2)
| Title |
|---|
| 便携式固相微萃取现场采样装置及其应用;陶萍 等;《分析仪器》;20041231(第2期);第37-40页 * |
| 固相微萃取现场流动采样装置;许栋应 等;《分析化学（ FENXI HUAXUE） 仪器装置与实验技术》;20021130;第30卷(第11期);第1394-1398页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104697822A (en) | 2015-06-10 |
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