CN104697822A - Expired air sampling bottle for solid phase microextraction - Google Patents
Expired air sampling bottle for solid phase microextraction Download PDFInfo
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- CN104697822A CN104697822A CN201510044964.0A CN201510044964A CN104697822A CN 104697822 A CN104697822 A CN 104697822A CN 201510044964 A CN201510044964 A CN 201510044964A CN 104697822 A CN104697822 A CN 104697822A
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- bottle
- vacuum valve
- phase microextraction
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- outlet pipe
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Abstract
The invention relates to an expired air sampling bottle for solid phase microextraction. The expired air sampling bottle comprises an air inlet pipe, a first vacuum valve, a bottle body, an air outlet pipe, a second vacuum valve and a solid phase microextraction connecting opening, wherein the air inlet pipe is located at one end of the bottle body, and the first vacuum valve is used for sealing the air inlet pipe; the air outlet pipe is located at the other end of the bottle body, and the second vacuum valve is used for sealing the air outlet pipe; the solid phase microextraction connecting opening is formed in the middle of the bottle body, and mainly comprises a threaded end, an insulating pad and a threaded cap, and small holes are left in the middle of the threaded cap. The expired air sampling bottle is good in tightness, and can be used for collecting air expired from human bodies, and the sampling process is convenient; the sampling bottle is mainly made of glass, so that the impurity interference can be reduced; and the sampling bottle can be used with a solid phase microextraction technology.
Description
Technical field
The invention belongs to analysis detection field, be specifically related to a kind of expiration sampling bottle that can be used for solid-phase microextraction.
Background technology
Containing many kinds of volatile organic matters (VOCs) in human exhaled breath, these materials reflect metabolic processes and the health status of human body.The auxiliary diagnosis that VOCs in being exhaled by human body in recent years carries out disease more and more comes into one's own.Because the VOCs content in exhaling is very low, detect again after often needing to use solid phase micro-extraction technique to carry out enrichment to VOCs.Traditional method is generally first gather human exhaled breath with sampler bag, again solid-phase microextraction syringe needle is penetrated sampler bag to extract the VOCs in expiration, but sampler bag also can discharge some VOCs, interference will be brought to the testing result of specimen of breath like this.
Summary of the invention
The present invention deals with problems: reduce exhale sampling extraction process in may bring impurity interference, the expiration sampling bottle that a kind of impermeability of glass material is good is provided, this sampling bottle can with solid phase micro-extraction technique coupling.
Technical solution of the present invention: a kind of expiration sampling bottle that can be used for solid-phase microextraction, comprises admission line, the first vacuum valve, bottle, outlet pipe, the second vacuum valve and solid-phase microextraction connectivity port.Admission line is positioned at bottle one end, and the first vacuum valve is used for being sealed into feed channel, and the first vacuum valve is detachable, and its bonnet is with internal thread, and the glass mouth of pipe be attached thereto, with external thread, controls the opening and closing of admission line by rotating bonnet; Outlet pipe is positioned at the other one end of bottle, and the second vacuum valve is used for sealing outlet pipe, and the second vacuum valve also can be dismantled, identical with the first vacuum valve with the connected mode of the glass mouth of pipe, controls the opening and closing of outlet pipe by rotating bonnet; Solid-phase microextraction connectivity port is positioned in the middle of bottle, and it stays foraminate thread cap to form primarily of threaded end (TE), dottle pin and centre.The material of admission line, bottle, outlet pipe and threaded end (TE) is glass, and the main material of the first vacuum valve and the second vacuum valve is teflon, and dottle pin adopts pure silica gel material, to reduce the impurity interference that may cause.The length of threaded end (TE) is 1.4-1.6 (usually can get 1.5cm), and dottle pin is placed in the female lid of band, and leave aperture in the middle of lid, thread cap and threaded end (TE) can tighten sealing.Solid-phase microextraction syringe needle penetrates in bottle by little aperture dottle pin and extracts.
Use procedure of the present invention is as follows: experimenter is slowly blown in bottle by the disposable blow gun be connected with admission line, exhaling enters in expiration bottle by admission line, in bottle, original air is discharged from outlet pipe, blow a few implication to ensure that air is drained more, blow and completely tighten the first vacuum valve and the second vacuum valve immediately, then in expiration end alveolar, gas is out sealed in bottle.The expiration sampling bottle sealed after collection is taken back laboratory and carries out breath analysis, solid-phase microextraction syringe needle can be penetrated in bottle by the little aperture dottle pin in the middle of lid and VOCs is extracted.
The present invention's advantage is compared with prior art as follows: the container of glass material is relatively cleaner, the impurity interference that traditional sampling bag can be avoided to bring; Sampling bottle sealing is good, specimen of breath can be prevented to be subject to outside air and pollute; Sampling process is simple, and can with solid phase micro-extraction technique coupling.
Accompanying drawing explanation
Fig. 1 is the integrally-built diagrammatic cross-section of the present invention;
Fig. 2 is the diagrammatic cross-section of three assemblies in solid-phase microextraction connectivity port in the present invention;
Fig. 3 is the diagrammatic cross-section by rear for sealing of exhaling and solid phase micro-extraction technique coupling in the present invention.
Embodiment
As shown in Figure 1, 2, 3, a kind of expiration sampling bottle embodiment that can be used for solid-phase microextraction of the present invention comprises: admission line 1, first vacuum valve 2, bottle 3, outlet pipe 4, second vacuum valve 5 and solid-phase microextraction connectivity port 6.Admission line 1 is positioned at bottle 3 one end, first vacuum valve 2 is used for being sealed into feed channel 1, and the first vacuum valve 2 is detachable, and its bonnet is with internal thread, the glass mouth of pipe be attached thereto, with external thread, controls the opening and closing of admission line 1 by rotating bonnet; Outlet pipe 4 is positioned at bottle 3 one end in addition, second vacuum valve is used for sealing outlet pipe 4, second vacuum valve 5 also can be dismantled, identical with the first vacuum valve 2 with the connected mode of the glass mouth of pipe, controls the opening and closing of outlet pipe 4 by rotating bonnet; Solid-phase microextraction connectivity port 6 is positioned in the middle of bottle.As shown in Figure 2, solid-phase microextraction connectivity port 6 stays foraminate thread cap 9 to form by threaded end (TE) 7, dottle pin 8 and centre.Described centre is stayed in foraminate thread cap 9 and is placed sizeable dottle pin 8, and centre stays foraminate thread cap 9 to tighten sealing by internal thread and threaded end (TE) 7, and solid-phase microextraction syringe needle is penetrated in bottle 3 by the little aperture dottle pin 8 in the middle of lid and extracts.Admission line 1, bottle 3, outlet pipe 4 and threaded end (TE) 7 are formed by columned glass tube, wherein admission line 1 and outlet pipe 4 are all be formed by connecting by two orthogonal glass tubes, first vacuum valve 2 and the second vacuum valve 5 carry out air seal by O-ring seal, the material of valve and pipe interior contact portion is teflon, and dottle pin 8 adopts pure silica gel material.Because the organism of this type of material is separated out few, the pollution to exhaling can be reduced.Admission line 1 can connect disposable blow gun, facilitates experimenter to blow in bottle.The length of threaded end (TE) 7 is about 1.5cm, and its length is unsuitable oversize, because solid-phase microextraction needle length is certain, if threaded end (TE) is oversize, syringe needle will be difficult to reach in the middle of bottle 3, can not fully extract.
The course of work of the present invention is: experimenter is slowly blown in bottle 3 by the disposable blow gun be connected with admission line 1, exhaling enters in expiration bottle by admission line 1, in bottle, original air is discharged from outlet pipe 4, blow and completely tighten the first vacuum valve 2 and the second vacuum valve 5 immediately, then in expiration end alveolar, gas is out sealed in bottle.The expiration sampling bottle sealed after collection is taken back laboratory and carries out breath analysis, as shown in Figure 3, solid-phase microextraction syringe needle can be penetrated in bottle by the little aperture dottle pin 8 in the middle of lid 9 and VOCs is extracted.
There is provided above embodiment to be only used to describe object of the present invention, and do not really want to limit the scope of the invention.Scope of the present invention is defined by the following claims.Do not depart from spirit of the present invention and principle and the various equivalent substitutions and modifications made, all should contain within the scope of the present invention.
Claims (6)
1. can be used for an expiration sampling bottle for solid-phase microextraction, it is characterized in that comprising: admission line (1), the first vacuum valve (2), bottle (3), outlet pipe (4), the second vacuum valve (5) and solid-phase microextraction connectivity port (6); Admission line (1) is positioned at bottle (3) one end, first vacuum valve (2) is used for being sealed into feed channel (1), first vacuum valve (2) is detachable, its bonnet is with internal thread, the glass mouth of pipe be attached thereto, with external thread, controls the opening and closing of admission line (1) by rotating bonnet; Outlet pipe (4) is positioned at bottle (3) one end in addition, second vacuum valve (5) is used for sealing outlet pipe (4), second vacuum valve (5) also can be dismantled, identical with the first vacuum valve (2) with the connected mode of the glass mouth of pipe, the opening and closing of outlet pipe (4) are controlled by rotating bonnet; Solid-phase microextraction connectivity port (6) is positioned in the middle of bottle, it stays foraminate thread cap (9) to form primarily of threaded end (TE) (7), dottle pin (8) and centre, described centre is stayed in foraminate thread cap (9) and is placed sizeable dottle pin (8), centre stays foraminate thread cap (9) to tighten sealing by internal thread and threaded end (TE) (7), and solid-phase microextraction syringe needle penetrates extraction in bottle (3) by the little aperture dottle pin (8) in the middle of lid.
2. a kind of expiration sampling bottle that can be used for solid-phase microextraction according to claim 1, it is characterized in that: described admission line (1), bottle (3), outlet pipe (4), threaded end (TE) (7) are formed by columned glass tube, wherein admission line (1) and outlet pipe (4) are all be formed by connecting by two orthogonal glass tubes.
3. a kind of expiration sampling bottle that can be used for solid-phase microextraction according to claim 1, it is characterized in that: described admission line (1) is expiration admission passage, expiration admission passage upper end connects disposable blow gun.
4. a kind of expiration sampling bottle that can be used for solid-phase microextraction according to claim 1, it is characterized in that: the first vacuum valve (2) and the second vacuum valve (5) carry out air seal by O-ring seal, the material of valve and pipe interior contact portion is teflon.
5. a kind of expiration sampling bottle that can be used for solid-phase microextraction according to claim 1, is characterized in that: the length of described threaded end (TE) (7) is 1.4-1.6cm.
6. a kind of expiration sampling bottle that can be used for solid-phase microextraction according to claim 1, is characterized in that: described dottle pin (8) adopts pure silica gel material.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510044964.0A CN104697822B (en) | 2015-01-28 | 2015-01-28 | A kind of expiration sampling bottle available for SPME |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510044964.0A CN104697822B (en) | 2015-01-28 | 2015-01-28 | A kind of expiration sampling bottle available for SPME |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104697822A true CN104697822A (en) | 2015-06-10 |
| CN104697822B CN104697822B (en) | 2017-07-14 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510044964.0A Active CN104697822B (en) | 2015-01-28 | 2015-01-28 | A kind of expiration sampling bottle available for SPME |
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| Country | Link |
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| CN (1) | CN104697822B (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107036852A (en) * | 2016-02-03 | 2017-08-11 | 奎特龙设备有限公司 | Breath tester device |
| CN113447321A (en) * | 2021-08-17 | 2021-09-28 | 古海燕 | Oil refining chemical industry enterprise safety ring protects gaseous sampling device |
| CN114354736A (en) * | 2022-01-11 | 2022-04-15 | 深圳市步锐生物科技有限公司 | System and method for detecting metabolic difference foreign matters in exhaled air |
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| CN202903554U (en) * | 2012-10-29 | 2013-04-24 | 中国石油大学(北京) | High-pressure gas sampling device |
| CN203122406U (en) * | 2013-01-11 | 2013-08-14 | 安徽养和医疗器械设备有限公司 | Exhaled gas sampling device |
| CN203849059U (en) * | 2014-05-08 | 2014-09-24 | 李海涛 | Device for collecting pollutants in air |
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2015
- 2015-01-28 CN CN201510044964.0A patent/CN104697822B/en active Active
Patent Citations (5)
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| CN202903554U (en) * | 2012-10-29 | 2013-04-24 | 中国石油大学(北京) | High-pressure gas sampling device |
| CN203122406U (en) * | 2013-01-11 | 2013-08-14 | 安徽养和医疗器械设备有限公司 | Exhaled gas sampling device |
| CN203849059U (en) * | 2014-05-08 | 2014-09-24 | 李海涛 | Device for collecting pollutants in air |
| CN203874645U (en) * | 2014-06-13 | 2014-10-15 | 国家电网公司 | Absorption bottle device for experimental tail gas in laboratory |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107036852A (en) * | 2016-02-03 | 2017-08-11 | 奎特龙设备有限公司 | Breath tester device |
| CN107036852B (en) * | 2016-02-03 | 2022-03-08 | 奎特龙设备有限公司 | Expiration testing device |
| CN113447321A (en) * | 2021-08-17 | 2021-09-28 | 古海燕 | Oil refining chemical industry enterprise safety ring protects gaseous sampling device |
| CN114354736A (en) * | 2022-01-11 | 2022-04-15 | 深圳市步锐生物科技有限公司 | System and method for detecting metabolic difference foreign matters in exhaled air |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104697822B (en) | 2017-07-14 |
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