CN104663544A - Artificial indoor seedling breeding method for charybdis feriatus - Google Patents

Artificial indoor seedling breeding method for charybdis feriatus Download PDF

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CN104663544A
CN104663544A CN201510064119.XA CN201510064119A CN104663544A CN 104663544 A CN104663544 A CN 104663544A CN 201510064119 A CN201510064119 A CN 201510064119A CN 104663544 A CN104663544 A CN 104663544A
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magna zoea
zoea larva
water
crab
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CN104663544B (en
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邹雄
夏连军
岳彦峰
陆建学
马洪雨
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East China Sea Fishery Research Institute Chinese Academy of Fishery Sciences
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
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    • Y02A40/81Aquaculture, e.g. of fish

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Abstract

The invention relates to an artificial indoor seedling breeding method for charybdis feriatus and belongs to field of charybdis feriatus cultivation. The method comprises the following three steps: preparing a breeding pond; hatching larvae, and putting the larvae into the pond; breeding seedlings. By taking the technical measures of water quality regulation, water temperature regulation, salinity regulation, illumination regulation, bait reinforcement and the like in the indoor breeding pond, artificial indoor seedling breeding is carried out on the charybdis feriatus. The artificial indoor seedling breeding method for the charybdis feriatus has the technical advantages that the specification of the seedlings is neat, the survival rate is high, and the vitality is high; the technology is mature, safe and easy to master, can be quickly absorbed and mastered by farmers, and is applied to development of large-scale artificial seedling breeding; the healthy and quick development of national artificial seedling breeding jobs for the charybdis feriatus is facilitated prospectively.

Description

The method of a kind of Xiu Ban Charybdis artificial room nursery
Technical field
The invention belongs to Xiu Ban Charybdis and cultivate field, the method for particularly a kind of Xiu Ban Charybdis artificial room nursery.
Background technology
Xiu Ban Charybdis (Charybdis feriatus) is the large-scale sea crabs class that a kind of economic worth is higher, is mainly distributed in southern East China Sea and South China Sea in China.Xiu Ban Charybdis delicious meat, mouthfeel is good, individuality is large, color red gorgeous celebrating, be of high nutritive value, be regarded as high-grade precious marine product, the market price one is to high.Xiu Ban Charybdis is one of four large economic crab of Western Pacific-the most applicable cultivation in the Indian Ocean.At present, the commodity crab overwhelming majority that market is sold derives from natural sea area, rarely has the commodity crab propagated artificially.By the change of overfishing and briny environment, Xiu Ban Charybdis wild resource is damaged, and Population and catch decline year by year.And along with Chinese society expanding economy, the raising of people's living standard, constantly increases the eating requirements of Xiu Ban Charybdis, and the quantity of the catch of its natural waters can not meet the demand in market.
Therefore, be necessary that the artificial seed carrying out Xiu Ban Charybdis breeds research, to promote that wild resource protection and sustainable use, alleviation relation between market supply and demand are of great importance.
Application number is 201010622099.0, publication number is a kind of method that the method for the Chinese patent application Xiu Ban Charybdis artificial breeding of CN 102106277A discloses Xiu Ban Charybdis artificial breeding, artificial culture or buy Xiu Ban Charybdis ovigerous crab, the inflation of band water is transported to nursery, after antifungal sterilization, put into hatchery hatching; Magna zoea larva after hatching, to add in EDTA (2-8mg/L) and heavy metal ion in water, utilizes phototaxis to carry out young separation, and the energetic young proceeds to be cultivated pond and cultivate; When Magna zoea larva is developed to megalops larva, by hang anti-residual mesh sheet, reduce unit of water body seedling density, strengthen aeration quantity, strengthen daily ration, feeding quantity carry out cutting one another's throat between anti-megalops larva, young crab and Magna zoea larva; The first phase-third phase young crab, be Xiu Ban Charybdis juvenile crab, can emerge.But this invention walks seedling density, bait feeding amount, feeding method, quantity of exchanged water with the present invention cultivating pond, there is greatly difference the aspects such as intensity of illumination.And there is not the technological approaches mentioning disease control in seedling raising process, megalops larva and the aspect such as young crab phase bait feeding amount and quantity of exchanged water in this invention, therefore can not ensure normally carrying out of nursery.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of Xiu Ban Charybdis artificial room breeding method, and the method has that seed size is neat, survival rate is high, energetic; Technology maturation, safety, easily to grasp, can by the advantage of raiser's rapid digestion and grasp.
The method of a kind of Xiu Ban Charybdis artificial room of the present invention nursery, carry out according to following step:
Cultivation pond 30mg/L potassium permanganate, as cultivation pond, is sterilized 3 hours by A, the indoor half buried cement pit of employing, then washes away totally rear water inlet with 200 mesh sieve tulle filtering seas, depth of water 80cm.The water Inversion phenomenon of Chi Shui and the condition of culture of close crab are consistent, and use the EDTA of 5mg/L ~ 10mg/L simultaneously, and throw in Micro Algae to density 12 ~ 150,000/m 3water body.
If B, ovigerous crab have embryonic development to the later stage, in time ovigerous crab should be moved to hatching pail.Each hatching pail puts oogenesis parent crab 1, and micro-shape inflation of boiling, keeps darker light; Seawater in hatching pail is all replaced by fresh seawater by every morning, and Yi razor clam of throwing something and feeding; Ovigerous crab is pulled out after hatching Magna zoea larva by ovigerous crab, and utilizes phototaxis to carry out young separation, and nursery is carried out in the cultivation pond proceeded to by the energetic young described in steps A.
C, cultivation pond in, the main bait biology of Magna zoea larva I-II phase is wheel animalcule, Magna zoea larva III-VI phase artemia nauplii of throwing something and feeding is main, wheel animalcule is auxiliary, need therebetween progressively to increase the feeding volume of halogen worm without joint body, and the feeding volume of wheel animalcule gradually reduces, stop throwing something and feeding completely to Magna zoea larva IV end of term, the Magna zoea larva VI after date phase adds appropriate artemia imago.Megalopa stage is thrown something and fed the shrimp, shellfish meat or the flesh of fish etc. that rub.After the metamorphosis of Magna zoea larva is megalops larva, the mutual residual phenomenon between the young is serious.The attachments such as large mesh polyethylene mesh sheet can be thrown in water and at the bottom of pond, cut one another's throats for young attachment, minimizing; Strengthen aeration quantity simultaneously, and ensure the sufficient supplies of bait.Water is not changed, the 10cm that the adds water every day also inflation in wavy during Magna zoea larva I-II.From Magna zoea larva III phase, change water, and increase quantity of exchanged water and boiling water shape aeration quantity gradually.The prevention of disease will be noted in whole seedling raising process.Cultivate the first phase-third phase young crab, be Xiu Ban Charybdis juvenile crab, can emerge.
Area 14 ~ the 16m of the buried cement pit in indoor half in described steps A 2, be 1.5 ~ 1.8m deeply, install aerating device according to every square of 1 gas head and from the standard of 5cm at the bottom of pond, continuous charge in cultivating process, dissolved oxygen reaches more than 4mg/L, water temperature 27 ~ 30 DEG C, salinity 25 ~ 30.
In described step B embryonic development to the later stage be crab embryo heartbeat to 150 times/more than min, the high 1.2m of bucket of hatching pail, volume is 0.6m 3.The young density of cultivating in pond is 100,000/m 3left and right, intensity of illumination should control at 3000Lx ~ 10000Lx, avoids high light direct projection, in order to avoid cause the young become light, increase mutual residual probability.
Control water temperature 27-30 DEG C in described step B, salinity is 25-30, between pH value 7.0 to 9.0.
Utilize phototaxis to carry out the young in described step B to be separated and to refer to the young weak to the energetic young and vigor is separated, directly over distance hatching pail, 30cm eminence opens the incandescent lamp of a 150W, stop inflation, leave standstill 20 minutes, make the energetic young become light float, and sinking to bottom hatching pail under the young of dead ovum and vigor difference, namely the top layer high-quality young is transferred to cultivates pond nursery.
Described step C Magna zoea larva I-II phase main bait biology is wheel animalcule, the density of wheel animalcule is 15/about ml, after this, throw something and feed 1 ~ 2 time every day according to actual conditions, Feeding time be morning 8:00 and afternoon 4:00, wheel animalcule is remained between 15/ml ~ 20/ml cultivating the density in pond.Magna zoea larva III-VI phase artemia nauplii of throwing something and feeding is main, wheel animalcule is auxiliary, need therebetween progressively to increase the feeding volume of halogen worm without joint body, Magna zoea larva III phase is 5/ml, Magna zoea larva IV phase is 10/ml, Magna zoea larva V phase is 15/ml, be 20/ml before Magna zoea larva VI phase, progressively be increased to the 25/ml in Magna zoea larva VI end of term, and the feeding volume of wheel animalcule gradually reduces, Magna zoea larva III phase is 15/ml, be 10/ml before Magna zoea larva IV phase, stop completely throwing something and feeding to Magna zoea larva IV end of term, the Magna zoea larva VI after date phase adds appropriate artemia imago, density is 0.5/ml.Megalopa stage is thrown something and fed the shrimp, shellfish meat or the flesh of fish etc. that rub, megalopa stage day feeding volume be 150% of crab body body weight, the young crab phase is 200% of young body weight.Each phase feeding volume controls flexibly with the young residual bait etc. of ingesting in situation, water.
Water is not changed, the 10cm that adds water every day also trace inflation during described step C Magna zoea larva I-II.From Magna zoea larva III phase, change water, and increase quantity of exchanged water and aeration quantity gradually.Each every day phase, quantity of exchanged water was respectively: Magna zoea larva III phase-30%; Magna zoea larva IV phase-40%; Magna zoea larva V phase-50%; Magna zoea larva VI phase-80%; Megalops larva-100%.Notice when changing water that the temperature difference is less than 0.5 DEG C.The Magna zoea larva VI after date phase starts as strong inflation, and Chi Shui is boiling-like.Change the water time be morning 7:00 and afternoon 3:00.
In described step C, water temperature 27-30 DEG C, salinity is 25-30, pH value 7.0-0.9, and intensity of illumination controls at 3000Lx ~ 10000Lx, more than dissolved oxygen 4mg/L.
Described step C, prophylactic agent is the conventional medicines such as Enrofloxacin HCL, Florfenicol, povidone iodine.Be used alternatingly, make that germ is unlikely to develop immunity to drugs.General consumption is at 1g/m 3~ 2g/m 3if morbidity can strengthen dose.
1st jaw foot and the 2nd jaw foot exopodite end bilimbate seta number carry out zoea principal character by stages.Magna zoea larva the I to VI phase the 1st jaw foot exopodite end bilimbate seta number is respectively 4,6,8,9-10,13-14 and 15-16; 2nd jaw foot exopodite end tool bilimbate seta number is respectively 4,6,8,10-11,14-15 and 17-18.Megalops larva is characterized as: carapace circle is blunt, and periproct trailing edge bristle number is 7.The young crab phase: build is like adult, and belly respectively saves and is curvedly affixed on below carapace, the tooth that cephalolateral margin tool 6 grades of carapace are large, margo frontalis is tool 6 tooth also.
Beneficial effect
The present invention by technical measures such as regulation and control water quality, water Inversion phenomenon, illumination, bait strengthenings, carries out rust spot Charybdis artificial room nursery in indoor culture pond.One is rational food density, both ensure that the energy required for the normal development by metamorphosis of the Xiu Ban Charybdis young, again reduces environmental pressure and ensures the good of water quality; Two is rational intensities of illumination, ensure that the needs that the young preys on, can prevent again the young become light assemble, cause hypoxia death; Three is suitable quantity of exchanged water, provides the condition of water quality of high-quality.
The extensive high-quality of the present invention cultivates that Xiu Ban Charybdis seed seed size is neat, survival rate is high, energetic; To propagate artificially for Xiu Ban Charybdis and Xiu Ban Charybdis stock enhancement is released, seed is provided.
Embodiment
In order to be technological means of the present invention, feature, reach and be easy to understand with effect, below in conjunction with specific embodiment, set forth the present invention further.
Embodiment 1
A kind of Xiu Ban Charybdis artificial room breeding method, comprises the following steps:
(1) cultivate pond to prepare:
In cultivation pond, long 5m, wide 3m, degree of depth 1.5m, if 15 gas stones (Fengxin Heng Anqi stone Co., Ltd full corundum gas stone (40*40*4)), make dissolved oxygen reach more than 4mg/L.After using 30mg/L potassium permanganate to sterilize 3 hours, cleaning pool wall.Inject fresh husky filtering sea, height of water level is 80cm.Cultivating workshop water temperature is 28.5 DEG C, seawater salinity 30.Use the EDTA of 5mg/L simultaneously, and throw in Micro Algae to density 130,000/m 3water body, makes water body be micro-green.
(2) hatching larvae enters pond
Embryo heartbeat being reached 150 times/min ovigerous crab, to be moved to volume be 0.6m 3hatching pail.Each hatching pail puts oogenesis parent crab 1, and micro-shape inflation of boiling, hides sunshade net; Yi razor clam, 100% of throwing something and feeding every day changes water; Cultivate two days later, ovigerous crab hatches Magna zoea larva, is taken out by ovigerous crab, removes sunshade net, stops inflation, leaves standstill a few minutes, utilizes phototaxis to carry out young separation, the energetic young is proceeded to the ready cultivation pond of step (1) and carry out nursery.The young density of cultivating in pond is 100,000/m 3left and right, intensity of illumination should control at 3000Lx ~ 10000Lx.
(3) seed rearing
During seed rearing, fresh food of mainly throwing something and feeding: wheel animalcule, halogen worm etc., and throw in Micro Algae to density 130,000/m 3water body, makes water body be micro-green.Bait feeding is thrown something and fed 1 ~ 2 time according to actual conditions every day, Feeding time be morning 8:00 and afternoon 4:00, change the water time be morning 7:00 and afternoon 3:00.
During seed rearing concrete bait and feeding method as follows.Magna zoea larva I-II phase wheel animalcule remains between 15/ml ~ 20/ml cultivating the density in pond, does not change water, and every morning, 7:00 added water 10cm, and trace inflation, Magna zoea larva I phase first day is according to 1g/m 3full pool spilling head Enrofloxacin HCL.Magna zoea larva III-VI phase artemia nauplii of throwing something and feeding is main, and wheel animalcule is auxiliary, and needing therebetween progressively increases the feeding volume of halogen worm without joint body.Magna zoea larva III phase starts the artemia nauplii 5/ml of just having hatched that throws something and feeds, and wheel animalcule density is 15/ml, and quantity of exchanged water is that 30%, Magna zoea larva III phase first day is according to 1g/m 3full pool spilling head Florfenicol; Magna zoea larva IV phase artemia nauplii is 10/ml, and early stage, wheel animalcule density was 10/ml, and later stage stopping is thrown something and fed, and quantity of exchanged water is 40%; Magna zoea larva V phase artemia nauplii is 15/ml, and quantity of exchanged water is that 50%, Magna zoea larva V phase first day is according to 1g/m 3full pool spilling head povidone iodine; Early stage Magna zoea larva VI phase, halogen worm was 20/ml, and be progressively increased to the 25/ml in Magna zoea larva VI end of term, and add appropriate artemia imago, density is 0.5/ml.Quantity of exchanged water is 80%, inflates by force, and Chi Shui is boiling-like.Megalopa stage is thrown something and fed the shrimp, shellfish meat or the flesh of fish etc. that rub, megalopa stage day feeding volume be 150% of crab body body weight, the young crab phase is 200% of young body weight, and quantity of exchanged water is 100%.After the metamorphosis of Magna zoea larva is megalops larva, the mutual residual phenomenon between the young is serious.Throw in the attachments such as large mesh polyethylene mesh sheet in water and at the bottom of pond, cut one another's throats for young attachment, minimizing; Strengthen aeration quantity simultaneously, and ensure the sufficient supplies of bait.Cultivate the first phase-third phase young crab, be Xiu Ban Charybdis juvenile crab, can emerge.
(4) fry rearing result
In September, 2014-November, the rust spot Charybdis ovigerous crab that oneself is cultivated, use 4, indoor culture pond, indoor total seedling water 60 squares, the cultivation time is 45 days, according to as above method nursery 2 batches, cultivate altogether the first phase-third phase young crab 400,000.
Reference examples:
In June, 2014, the Xiu Ban Charybdis ovigerous crab that oneself is cultivated, use 4, indoor culture pond, indoor total seedling water 60 squares, according to as above method nursery 1 batch, during seed rearing, only bait feeding has following difference: Magna zoea larva I-II phase wheel animalcule remains between 10/ml ~ 15/ml cultivating the density in pond; Magna zoea larva III phase starts the artemia nauplii 5/ml of just having hatched that throws something and feeds, and stops throwing something and feeding wheel animalcule.Find in seedling raising process that Magna zoea larva VI phase is not neat to megalopa stage metamorphosis, megalops larva is severe to the cannibalisation of Magna zoea larva VI phase, this nursery only cultivate the first phase-third phase young crab 40,000.
In July, 2014, the Xiu Ban Charybdis ovigerous crab that oneself is cultivated, use 4, indoor culture pond, indoor total seedling water 60 squares, according to as above method nursery 1 batch, owing to being subject to the impact of typhoon weather, cultivate pond intensity of illumination daytime lower than 3000Lx, seawater salinity bust is 15, cause this nursery only cultivate the first phase-third phase young crab 50,000, and seed size is not neat, seed vigor is poor.
Below the preferred embodiment of the invention is illustrated, but the invention is not limited to described embodiment, those of ordinary skill in the art also can make all equivalent modification or replacement under the prerequisite without prejudice to the invention spirit, and these equivalent modification or replacement are all included in the application's claim limited range.

Claims (9)

1. to become rusty the method for spot Charybdis artificial room nursery, carry out according to following step:
A, the indoor half buried cement pit of employing are as cultivation pond, cultivation pond 30mg/L potassium permanganate is sterilized 3 hours, then washes away clean rear water inlet with 200 mesh sieve tulle filtering seas, depth of water 80cm, use the EDTA of 5mg/L ~ 10mg/L simultaneously, and throw in Micro Algae to density 12 ~ 150,000/m 3water body;
Ovigerous crab, to the later stage, is moved to hatching pail by B, ovigerous crab embryonic development in time; Each hatching pail puts ovigerous crab 1, and micro-shape inflation of boiling, keeps darker light; Seawater in hatching pail is all replaced by fresh seawater by every morning, and Yi razor clam of throwing something and feeding; Ovigerous crab is pulled out after hatching Magna zoea larva by ovigerous crab, and utilizes phototaxis to carry out young separation, and nursery is carried out in the cultivation pond proceeded to by the energetic young described in steps A;
C, cultivation pond in, the main bait biology of Magna zoea larva I-II phase is wheel animalcule, Magna zoea larva III-VI phase artemia nauplii of throwing something and feeding is main, wheel animalcule is auxiliary, need therebetween progressively to increase the feeding volume of halogen worm without joint body, and the feeding volume of wheel animalcule gradually reduces, stop throwing something and feeding completely to Magna zoea larva IV end of term, the Magna zoea larva VI after date phase adds appropriate artemia imago; Megalopa stage throw something and feed rub shrimp, shellfish meat or the flesh of fish; After the metamorphosis of Magna zoea larva is megalops larva; Large mesh polyethylene mesh sheet attachment is thrown in water and at the bottom of pond; Water is not changed, the 10cm that the adds water every day also inflation in wavy during Magna zoea larva I-II; From Magna zoea larva III phase, change water, and increase quantity of exchanged water and the inflation of boiling water shape gradually; Cultivate the first phase-third phase young crab, be Xiu Ban Charybdis juvenile crab, can emerge; The prevention of disease will be noted in whole seedling raising process.
2. the method for a kind of Xiu Ban Charybdis artificial room according to claim 1 nursery, is characterized in that, the area 14 ~ 16m of the buried cement pit in indoor half in described steps A 2, be 1.5 ~ 1.8m deeply, install aerating device according to every square of 1 gas head and from the standard of 5cm at the bottom of pond, continuous charge in cultivating process, dissolved oxygen reaches more than 4mg/L, water temperature 27 ~ 30 DEG C, salinity 25 ~ 30.
3. the method for a kind of Xiu Ban Charybdis artificial room according to claim 1 nursery, is characterized in that, embryonic development described in step B to the later stage is that crab embryo heartbeat is to 150 times/more than min; The high 1.2m of bucket of described hatching pail, volume is 0.6m 3; Young density in described cultivation pond is 100,000/m 3left and right, intensity of illumination controls at 3000Lx ~ 10000Lx.
4. the method for a kind of Xiu Ban Charybdis artificial room according to claim 1 nursery, it is characterized in that, control water temperature 27-30 DEG C in described step B, salinity is 25-30, between pH value 7.0 to 9.0.
5. the method for basis-a kind of Xiu Ban Charybdis artificial room's according to claim 1 nursery, it is characterized in that, utilize phototaxis to carry out the young in described step B to be separated and to refer to the young weak to the energetic young and vigor is separated, directly over distance hatching pail, 30cm eminence opens the incandescent lamp of a 150W, stop inflation, leave standstill 20 minutes, make the energetic young become light float, and sinking to bottom hatching pail under the young of dead ovum and vigor difference, namely the top layer high-quality young is transferred to cultivates pond nursery.
6. the method for a kind of Xiu Ban Charybdis artificial room according to claim 1 nursery, it is characterized in that, described step C Magna zoea larva I-II phase main bait biology is wheel animalcule, the density of wheel animalcule is 15/about ml, after this, throw something and feed 1 ~ 2 time every day according to actual conditions, Feeding time be morning 8:00 and afternoon 4:00, make wheel animalcule remain between 15/ml ~ 20/ml cultivating the density in pond, Magna zoea larva III-VI phase artemia nauplii of throwing something and feeding is main, wheel animalcule is auxiliary, need therebetween progressively to increase the feeding volume of halogen worm without joint body, Magna zoea larva III phase is 5/ml, Magna zoea larva IV phase is 10/ml, Magna zoea larva V phase is 15/ml, be 20/ml before Magna zoea larva VI phase, progressively be increased to the 25/ml in Magna zoea larva VI end of term, and the feeding volume of wheel animalcule gradually reduces, Magna zoea larva III phase is 15/ml, be 10/ml before Magna zoea larva IV phase, stop completely throwing something and feeding to Magna zoea larva IV end of term, the Magna zoea larva VI after date phase adds appropriate artemia imago, density is 0.5/ml, megalopa stage throw something and feed rub shrimp, shellfish meat or the flesh of fish, megalopa stage day feeding volume be 200% of crab body body weight, the day first phase young crab phase feeding volume be 150% of crab body weight, second and third young day crab phase feeding volume be 100% of crab body weight.The first phase-third phase young crab, be Xiu Ban Charybdis juvenile crab, can emerge.
7. the method for a kind of Xiu Ban Charybdis artificial room according to claim 1 nursery, is characterized in that, does not change water during described step C Magna zoea larva I-II, the 10cm that adds water every day also trace inflation; From Magna zoea larva III phase, change water, and increase quantity of exchanged water and aeration quantity gradually; Each every day phase, quantity of exchanged water was respectively: Magna zoea larva III phase-30%; Magna zoea larva IV phase-40%; Magna zoea larva V phase-50%; Magna zoea larva VI phase-80%; Megalops larva-100%; The young crab phase-150%; When changing water, the temperature difference is less than 0.5 DEG C; The Magna zoea larva VI after date phase starts as strong inflation, and Chi Shui is boiling-like; Change the water time be morning 7:00 and afternoon 3:00.
8. the method for a kind of Xiu Ban Charybdis artificial room according to claim 1 nursery, is characterized in that, in described step C, water temperature 27-30 DEG C, salinity is 25-30, pH value 7.0-0.9, and intensity of illumination controls at 3000Lx ~ 10000Lx, more than dissolved oxygen 4mg/L.
9. the method for a kind of Xiu Ban Charybdis artificial room according to claim 1 nursery, it is characterized in that, described step C, prophylactic agent is Enrofloxacin HCL, Florfenicol or povidone iodine, is used alternatingly, and consumption is 1g/m 3~ 2g/m 3if morbidity can strengthen dose.
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CN106035164A (en) * 2016-06-16 2016-10-26 中国水产科学研究院下营增殖实验站 Method for improving breeding survival rate of Portunus trituberculatus larvae in northern areas
CN106852289A (en) * 2017-01-12 2017-06-16 淮海工学院 A kind of method of raising this Charybdis young survival rates
CN110338114A (en) * 2019-08-16 2019-10-18 浙江省海洋水产研究所 Portunus trituberculatus Miers anti-season isolating female from male culture method
CN110338113A (en) * 2019-08-16 2019-10-18 浙江省海洋水产研究所 Swimming crab season seeding method

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