CN104649760A - Soil high carbon-based additive prepared from fermented corn straw - Google Patents

Soil high carbon-based additive prepared from fermented corn straw Download PDF

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CN104649760A
CN104649760A CN201510059816.6A CN201510059816A CN104649760A CN 104649760 A CN104649760 A CN 104649760A CN 201510059816 A CN201510059816 A CN 201510059816A CN 104649760 A CN104649760 A CN 104649760A
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liquid
carbon back
soil
bacterium liquid
back additive
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CN104649760B (en
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陈泽鹏
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China National Tobacco Corp Guangdong Branch
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China National Tobacco Corp Guangdong Branch
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    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F17/00Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F11/00Other organic fertilisers
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C05G3/80Soil conditioners
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02WCLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00Technologies for solid waste management
    • Y02W30/40Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

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  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Health & Medical Sciences (AREA)
  • Soil Sciences (AREA)
  • Biochemistry (AREA)
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  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Fertilizers (AREA)

Abstract

The invention belongs to the technical field of soil conditioners, and in particular discloses a soil high carbon-based additive prepared from fermented corn straw. According to the nutritional component composition of corn straw, the corn straw is degraded by selecting a microorganism bacterium agent which consists of a specific formula, cellulose and lignin in the straw can be fully transformed, and thus organic matters capable of being absorbed by a plant root system can be obtained. By adopting the soil high carbon-based additive disclosed by the invention, the soil fertility can be greatly improved, and waste plant straw can also be reasonably used so as to avoid the occurrence of environmental pollution.

Description

The soil height carbon back additive that a kind of fermented maize stalk prepares
Technical field
The present invention relates to soil improvement agent technical field, particularly, relate to the soil height carbon back additive that a kind of fermented maize stalk prepares.
Background technology
Along with the raising of China's farm output, the output of agricultural crop straw is also being multiplied.Made according to the relevent statistics, the output of the current all kinds of agricultural crop straw in the whole nation is reaching 7 ~ 1,000,000,000 tons every year, and its quantity is equivalent to 60 times of Northern grassland output.For a long time, the process problem of agricultural crop straw is the problem making vast farmers and governments at all levels feel more headache always, past is due to reasons such as the shortage of evaluation and exploration technology, market information are inaccessible and peasant's autumn ploughing sowing time is of short duration, most stalk goes out of use in river course, rot or directly destroyed by fire in field in roadside, not only block river course and road traffic, and severe contamination environment cause the huge waste of resource.
Due to stalk not easily decomposition in a short time, cause stalk resource reasonably not developed for a long time, except being on a small quantity used for heap making compost for packing ring, pasture, part, major part has all been burnt as life fuel, in the area that plant husbandry is main, crop material has become the obstacle in production.So crop straw burning, throughout vast rural area, causes the serious waste of environmental pollution and organic resource thus.According to statistics, the crop material that the whole nation is used as also field and organic fertilizer only accounts for about 20% of total amount, burns and accounts for 17%, after straw burning, primary product is carbonic acid gas, carbon monoxide, the obnoxious flavoures such as sulfurous gas, these gases cause serious environmental pollution after entering air.Crop straw burning do four large harms: one is waste resource, causes large amount of organic not go back to field; Two is serious environment pollutions; Three is frequent initiation fire and traffic accident; Four is destroy farmland ecosystem material cycle, causes soil fertility to decline.
Impact for the meticulousr crop of those nutritional requirements after soil and fertilizer declines is large especially, such as tobacco, tobacco is very strong to the adaptability of soil, from light sandy soil to heavy clay, from sour earth to alkaline soil, from light gray to the various soil such as yellow, red, black is native, the needs that tobacco growing is grown can be met.But the tobacco leaf local flavor that different soils is produced is different, the peppery people that chokes of some hardships, some aromatic alcohols and, what have is greyish white bright, the but grey black had stop working.So consider from the angle of quality of tobacco, the requirement of tobacco leaf production to edaphic condition is stricter.In order to improve the fertility of tobacco planting soil, tobacco grower tries to add in soil by after the crushed stalk such as paddy rice, corn, but, because stalk needs long time just can be completely broken down in soil, so stalk add the fertility that not only can not increase soil in time, what can affect tobacco on the contrary cultivates management.
At present, the multiple-microorganism Treating straw that is mixed is utilized to be popular solution.But the vigor of current published straw degradative bacterium is not very high, and is combined by multiple-microorganism, and the effect between microorganism is not simple additive effect, can produce antagonistic action time some bacterium mixes on the contrary.
Goal of the invention
The present invention, in order to overcome above-mentioned deficiency of the prior art, provides a kind of soil height carbon back additive; This additive is added in plant soil, especially in Tobacco, effectively can improve the fertility of soil, promote the growth of plant, and high carbon back additive of the present invention can also reduce the use of mineral manure.
To achieve these goals, the present invention is achieved by following scheme:
A kind of composite fungus agent of degrading maize straws, each bacterium liquid containing following weight percent: 10 ~ 30% penicillium oxalicum liquid, 5 ~ 20% succsinic acid thread bacillus bacterium liquid, 10 ~ 30% long handle Trichoderma liquid, 5 ~ 20% Bacillus licheniformis liquid, 15 ~ 35% rhodococcus bacterium liquid, in each bacterium liquid, viable count is 1 ~ 9 × 10 8individual/gram.
The trophic component more complicated of maize straw, the Mierocrystalline cellulose comprising 40%, 20 ~ 30% hemicelluloses, 24% xylogen, the crude protein of surplus, crude fat, nitrogen-free extract.The present invention is according to the trophic component of maize straw, through a large amount of shaker test, carry out reasonable compatibility to bacterial strain, the microbial inoculum formula of acquisition is reasonable, between each bacterial strain, there is good synergistic effect, the complex micro organism fungicide obtained has that strain combinations is new, structure is steady, enzyme lives strong, bacterium number high.Wherein, the thread bacillus of succsinic acid is one of rumen bacteria, it can not only produce multifilament element enzyme body (comprising cellulase, inscribe hemicellulase and circumscribed hemicellulase), the cellulose binding protein be positioned in a large number on cell outer surface can also be produced, this bacterium is made to stick on stalk cellulose, improve the degradation capability to stalk cellulose, therefore, the thread bacillus of succsinic acid of the present invention can carry out efficient degradation to Mierocrystalline cellulose, hemicellulose; And a large amount of cellulase synthesis of the long handle mould energy of wood, hemicellulase, its cellulase comprises exoglucanase, endoglucanase, but the exoglucanase activity produced is low, and the activity of the exoglucanase that the thread bacillus of penicillium oxalicum, succsinic acid produces is high, so, can efficient degradation Mierocrystalline cellulose and xylogen by triplicity.In addition, in growth metabolism process, Bacillus licheniformis can produce the multiple enzymatic factor, increases the activity of other enzymes, and can produce activity resistent material, has and regulates flora to coordinate, suppress the effect of varied bacteria growing breeding; Rhodococcus energy lignin degrading, play the barrier action of breaking xylogen and protein and being formed, make Mierocrystalline cellulose and hemicellulose be exposed, in addition simultaneously, rhodococcus can also be degraded many halogenated biphenyls, hydro carbons, coal and petroleum substance, plays the effect that edatope is repaired.The present invention utilizes the synergistic effect between above-mentioned five kinds of bacterial classifications, the composite bacteria obtained can degrading maize straws more efficiently, effectively can also improve the physico-chemical property of soil simultaneously, promote crop growth, induction crop strengthens resistance, strengthen cold-resistant, drought resisting, disease-resistant and anti-adversity ability, increase yield, improves product quality.
Preferably, described composite fungus agent contains each bacterium liquid of following weight percent: 15 ~ 25% penicillium oxalicum liquid, 10 ~ 20% succsinic acid thread bacillus bacterium liquid, 20 ~ 30% long handle Trichoderma liquid, 10 ~ 20% Bacillus licheniformis liquid, 20 ~ 30% rhodococcus bacterium liquid.
More preferably, described composite fungus agent contains each bacterium liquid of following weight percent: 20% penicillium oxalicum liquid, 15% succsinic acid thread bacillus bacterium liquid, 25% long handle Trichoderma liquid, 15% Bacillus licheniformis liquid, 25% rhodococcus bacterium liquid.
A kind of soil height carbon back additive, prepare by the following method: pile up after maize straw being cut into stalk section, mixture is obtained after being mixed with wheat-flour by mix bacterium agent as above, mixture is joined stalk heap, regulate heap body water-content, normal temperature fermentation obtains soil height carbon back additive.
When mix bacterium agent of the present invention is used for degrading maize straws, nutrition in stalk is poorly degraded, in order to make each microorganism energy amount reproduction, need to provide suitable nutrition to various microorganism, the present invention is found by research, and wheat-flour can provide good nutritional needs for each bacterial strain, and if the words of wheat bran, Semen Maydis powder, effect is just so not good, and this may be because each microbial strains of the present invention more easily absorbs the nutritive substance in wheat-flour.
Preferably, described composite fungus agent and the mixing quality of wheat-flour are than being 1:5 ~ 10.
Preferably, described mixture be by weight percentage 1 ~ 10% add to stalk heap in.
More preferably, described mixture be by weight percentage 2 ~ 5% add to stalk heap in.
Under suitable moisture condition, due to the various extracellular enzymes increases that the reproduction speed of various microorganism is accelerated, secreted, so the also corresponding raising of the ability of the materials such as degraded cellulose, preferably, heap body water-content is regulated to be 50 ~ 65%.
Preferably, the condition of described fermentation is that normal temperature fermentation carried out turning after 2 ~ 3 days; Then turning in every 10 ~ 15 days 1 time, altogether turning 2 times; Namely soil height carbon back additive is obtained after last turning completes.
Compared with prior art, the present invention has following beneficial effect:
Contriver is according to the trophic component of maize straw, through a large amount of shaker test, carry out reasonable compatibility to bacterial strain, the microbial inoculum formula of acquisition is reasonable, between each bacterial strain, there is good synergistic effect, the complex micro organism fungicide obtained has that strain combinations is new, structure is steady, enzyme lives strong, bacterium number high.This complex micro organism fungicide can accelerate the degraded of maize straw, has efficient degradation property to being difficult to degrading maize straws.
Soil height carbon back additive of the present invention effectively can increase soil organic matter content, improvement soil, culture fertility, especially to alleviating Tobacco nitrogen, phosphorus, potash fertilizer contradiction out of proportion, making up phosphorus, potassium fertilizer deficiency plays an important role.In addition, soil height carbon back additive of the present invention can also improve soil materialization property, increases soil aggregate effect, strengthens Soil Humus content, improves Soil Microorganism activity and enzymic activity.Therefore the plantation output of tobacco is improved.
Embodiment
Further illustrate content of the present invention below in conjunction with specific embodiment, but should not be construed as limitation of the present invention.Without departing from the spirit and substance of the case in the present invention, the simple modification do the inventive method, step or condition or replacement, all belong to scope of the present invention; If do not specialize, the conventional means that technique means used in embodiment is well known to those skilled in the art.
The thread bacillus of penicillium oxalicum of the present invention, succsinic acid, long handle wood are mould, Bacillus licheniformis, rhodococcus are common commercially available bacterial classification.
Embodiment 1
S1. mould to penicillium oxalicum, the thread bacillus of succsinic acid, long handle wood, Bacillus licheniformis, rhodococcus activated respectively, be cultured to viable count in bacterium liquid and reach 8 × 10 8individual/gram;
S2. 75g penicillium oxalicum liquid, 100g succsinic acid thread bacillus bacterium liquid, 50g long handle Trichoderma liquid, 100g Bacillus licheniformis liquid, 175g rhodococcus bacterium liquid are mixed to obtain mixed bacteria liquid, the wheat-flour of mixed bacteria liquid and 2.5kg is mixed to get composite fungus agent.
S3. a soil height carbon back additive, prepares by the following method: pile up after 100kg maize straw being cut into the long stalk section of about 25cm, add mixture by stalk weight percent 3%, regulates heap body water-content 55%; Normal temperature fermentation carried out turning after 3 days; Then turning in every 15 days 1 time, altogether turning 2 times; Namely soil height carbon back additive is obtained after last turning completes.
Embodiment 2
S1. mould to penicillium oxalicum, the thread bacillus of succsinic acid, long handle wood, Bacillus licheniformis, rhodococcus activated respectively, be cultured to viable count in bacterium liquid and reach 1 × 10 8individual/gram;
S2. 300g penicillium oxalicum liquid, 200g succsinic acid thread bacillus bacterium liquid, 300g long handle Trichoderma liquid, 50g Bacillus licheniformis liquid, 150g rhodococcus bacterium liquid are mixed to obtain mixed bacteria liquid, the wheat-flour of mixed bacteria liquid and 8kg is mixed to get composite fungus agent.
S3. a soil height carbon back additive, prepares by the following method: pile up after 100kg maize straw being cut into the long stalk section of about 25cm, add mixture by stalk weight percent 9%, regulates heap body water-content 55%; Normal temperature fermentation carried out turning after 3 days; Then turning in every 15 days 1 time, altogether turning 2 times; Namely soil height carbon back additive is obtained after last turning completes.
Embodiment 3
S1. mould to penicillium oxalicum, the thread bacillus of succsinic acid, long handle wood, Bacillus licheniformis, rhodococcus activated respectively, be cultured to viable count in bacterium liquid and reach 5 × 10 8individual/gram;
S2. 100g penicillium oxalicum liquid, 75g succsinic acid thread bacillus bacterium liquid, 150g long handle Trichoderma liquid, 75g Bacillus licheniformis liquid, 125g rhodococcus bacterium liquid are mixed to obtain mixed bacteria liquid, the wheat-flour of mixed bacteria liquid and 2.5kg is mixed to get composite fungus agent.
S3. a soil height carbon back additive, prepares by the following method: pile up after 100kg maize straw being cut into the long stalk section of about 25cm, add mixture by stalk weight percent 3%, regulates heap body water-content 60%; Normal temperature fermentation carried out turning after 3 days; Then turning in every 10 days 1 time, altogether turning 2 times; Namely soil height carbon back additive is obtained after last turning completes.
Embodiment 4
S1. mould to penicillium oxalicum, the thread bacillus of succsinic acid, long handle wood, Bacillus licheniformis, rhodococcus activated respectively, be cultured to viable count in bacterium liquid and reach 3 × 10 8individual/gram;
S2. 90g penicillium oxalicum liquid, 30g succsinic acid thread bacillus bacterium liquid, 180g long handle Trichoderma liquid, 120g Bacillus licheniformis liquid, 180g rhodococcus bacterium liquid are mixed to obtain mixed bacteria liquid, the wheat-flour of mixed bacteria liquid and 5.4kg is mixed to get composite fungus agent.
S3. a soil height carbon back additive, prepares by the following method: pile up after 100kg maize straw being cut into the long stalk section of about 20cm, add mixture by stalk weight percent 6%, regulates heap body water-content 65%; Normal temperature fermentation carried out turning after 3 days; Then turning in every 15 days 1 time, altogether turning 2 times; Namely soil height carbon back additive is obtained after last turning completes.
Comparative example 1
S1. by penicillium oxalicum, the thread bacillus of succsinic acid, long handle wood is mould, Bacillus licheniformis activates respectively, be cultured to viable count in bacterium liquid reaches 5 × 10 8individual/gram;
S2. 100g penicillium oxalicum liquid, 75g succsinic acid thread bacillus bacterium liquid, 150g long handle Trichoderma liquid, 75g Bacillus licheniformis liquid are mixed to obtain mixed bacteria liquid, the wheat-flour of mixed bacteria liquid and 2.625kg is mixed to get composite fungus agent.
S3. a soil height carbon back additive, prepares by the following method: pile up after 100kg maize straw being cut into the long stalk section of about 25cm, add mixture by stalk weight percent 3%, regulates heap body water-content 60%; Normal temperature fermentation carried out turning after 3 days; Then turning in every 10 days 1 time, altogether turning 2 times; Namely soil height carbon back additive is obtained after last turning completes.
Comparative example 2
S1. mould for long handle wood, Bacillus licheniformis, rhodococcus activated respectively, be cultured to viable count in bacterium liquid and reach 5 × 10 8individual/gram;
S2. 150g long handle Trichoderma liquid, 75g Bacillus licheniformis liquid, 125g rhodococcus bacterium liquid are mixed to obtain mixed bacteria liquid, the wheat-flour of mixed bacteria liquid and 2.675kg is mixed to get composite fungus agent.
S3. a soil height carbon back additive, prepares by the following method: pile up after 100kg maize straw being cut into the long stalk section of about 25cm, add mixture by stalk weight percent 3%, regulates heap body water-content 60%; Normal temperature fermentation carried out turning after 3 days; Then turning in every 10 days 1 time, altogether turning 2 times; Namely soil height carbon back additive is obtained after last turning completes.
Comparative example 3
S1. mould to penicillium oxalicum, long handle wood, Bacillus licheniformis, rhodococcus activated respectively, be cultured to viable count in bacterium liquid and reach 5 × 10 8individual/gram;
S2. 100g penicillium oxalicum liquid, 150g long handle Trichoderma liquid, 75g Bacillus licheniformis liquid, 125g rhodococcus bacterium liquid are mixed to obtain mixed bacteria liquid, the wheat-flour of mixed bacteria liquid and 2.575kg is mixed to get composite fungus agent.
S3. a soil height carbon back additive, prepares by the following method: pile up after 100kg maize straw being cut into the long stalk section of about 25cm, add mixture by stalk weight percent 3%, regulates heap body water-content 60%; Normal temperature fermentation carried out turning after 3 days; Then turning in every 10 days 1 time, altogether turning 2 times; Namely soil height carbon back additive is obtained after last turning completes.
Comparative example 4
S1. mould to penicillium oxalicum, the thread bacillus of succsinic acid, long handle wood, Bacillus licheniformis, rhodococcus activated respectively, be cultured to viable count in bacterium liquid and reach 5 × 10 8individual/gram;
S2. 100g penicillium oxalicum liquid, 75g succsinic acid thread bacillus bacterium liquid, 150g long handle Trichoderma liquid, 75g Bacillus licheniformis liquid, 125g rhodococcus bacterium liquid are mixed to obtain mixed bacteria liquid, the wheat bran of mixed bacteria liquid and 2.5kg is mixed to get composite fungus agent.
S3. a soil height carbon back additive, prepares by the following method: pile up after 100kg maize straw being cut into the long stalk section of about 25cm, add mixture by stalk weight percent 3%, regulates heap body water-content 60%; Normal temperature fermentation carried out turning after 3 days; Then turning in every 10 days 1 time, altogether turning 2 times; Namely soil height carbon back additive is obtained after last turning completes.
Comparative example 5
S1. mould to penicillium oxalicum, the thread bacillus of succsinic acid, long handle wood, Bacillus licheniformis, rhodococcus activated respectively, be cultured to viable count in bacterium liquid and reach 5 × 10 8individual/gram;
S2. 100g penicillium oxalicum liquid, 75g succsinic acid thread bacillus bacterium liquid, 150g long handle Trichoderma liquid, 75g Bacillus licheniformis liquid, 125g rhodococcus bacterium liquid are mixed to obtain mixed bacteria liquid, the Semen Maydis powder of mixed bacteria liquid and 2.5kg is mixed to get composite fungus agent.
S3. a soil height carbon back additive, prepares by the following method: pile up after 100kg maize straw being cut into the long stalk section of about 25cm, add mixture by stalk weight percent 3%, regulates heap body water-content 60%; Normal temperature fermentation carried out turning after 3 days; Then turning in every 10 days 1 time, altogether turning 2 times; Namely soil height carbon back additive is obtained after last turning completes.
Application examples 1
The various composite fungus agents prepared by the S2 of embodiment 1 ~ 4 and comparative example 1 ~ 3 are forwarded to the bacterium amount that connects that volume percent is 2% and add shaking culture in Hutchinson substratum that corn stalk powder (5 order) is sole carbon source, within 25 days, nutrient solution is collected in cultivation, and with the rate of weight loss of the Van Voest method mensuration rice straw powder differing from heavy method and improvement, cellulose degradation rate, hemicellulose degradation rate and Lignin degradation rate, finally obtain the actual degradation effect comprehensive test result (see table 1) of various composite fungus agent to corn stalk powder.Table 1 result shows: the thread bacillus of penicillium oxalicum, succsinic acid, long handle wood are mould, Bacillus licheniformis, rhodococcus have significant synergistic function when degrading maize straws.
The various composite fungus agent of table 1. is to the degradation effect of corn stalk powder
Process Rate of weight loss Cellulose degradation rate Hemicellulose degradation rate Lignin degradation rate
Embodiment 1 86.7% 92.3% 90.8% 90.7%
Embodiment 2 82.7% 90.4% 89.6% 88.6%
Embodiment 3 92.1% 94.6% 95.7% 96.7%
Embodiment 4 84.8% 91.7% 92.7% 89.4%
Comparative example 1 76.2% 80.2% 79.4% 54.6%
Comparative example 2 65.4% 67.2% 66.4% 85.7%
Comparative example 3 70.5% 76.7% 76.4% 81.5%
Application examples 2
Test site: Shaoguan, Guangdong Province Nanxiong City
Test period: 2012 ~ 2013 years
Tobacco bred: K326
For examination soil: beef liver soil field
Testing program: the tobacco seedling that embodiment 1 ~ 4 and each treatment group of comparative example 1 ~ 5 all select 3 mu of growing ways identical is experimental subjects.Establish control treatment, control treatment, for comparing the management of normal tobacco cultivation, reduces by the normal rate of fertilizer application of 25% simultaneously; Embodiment 1 ~ 4 manages for comparing normal tobacco cultivation with each treatment group of comparative example 1 ~ 5, and reduce by the rate of fertilizer application of 25% but the various high carbon back additive prepared of interpolation 25%, other cultivation conditions are identical.Measure various soil height carbon back additive to the impact of tobacco growing, gather in the crops after tobacco maturation, measure tobacco per mu yield and the average yield per mu value of each treatment group, and, produce the flue-cured tobacco that obtains please the tobacco leaf expert that smokes panel test smoke panel test, measure the quality of the flue-cured tobacco that each treatment group obtains, result is as shown in table 2.
The tobacco results total amount of each treatment group of table 2 and quality evaluation
As shown in Table 2, output, quality and the output value etc. that high carbon back additive of the present invention adds tobacco leaf all is to a certain extent used.

Claims (9)

1. the composite fungus agent of a degrading maize straws, it is characterized in that, each bacterium liquid containing following weight percent: 10 ~ 30% penicillium oxalicum liquid, 5 ~ 20% succsinic acid thread bacillus bacterium liquid, 10 ~ 30% long handle Trichoderma liquid, 5 ~ 20% Bacillus licheniformis liquid, 15 ~ 35% rhodococcus bacterium liquid, in each bacterium liquid, viable count is 1 ~ 9 × 10 8individual/gram.
2. composite fungus agent according to claim 1, it is characterized in that, composite fungus agent contains each bacterium liquid of following weight percent: 15 ~ 25% penicillium oxalicum liquid, 10 ~ 20% succsinic acid thread bacillus bacterium liquid, 20 ~ 30% long handle Trichoderma liquid, 10 ~ 20% Bacillus licheniformis liquid, 20 ~ 30% rhodococcus bacterium liquid.
3. composite fungus agent according to claim 1, it is characterized in that, composite fungus agent contains each bacterium liquid of following weight percent: 20% penicillium oxalicum liquid, 15% succsinic acid thread bacillus bacterium liquid, 25% long handle Trichoderma liquid, 15% Bacillus licheniformis liquid, 25% rhodococcus bacterium liquid.
4. a soil height carbon back additive, it is characterized in that, prepare by the following method: pile up after maize straw being cut into stalk section, mixture is obtained after being mixed with wheat-flour by mix bacterium agent described in any one of claims 1 to 3, mixture is joined stalk heap, regulate heap body water-content, normal temperature fermentation obtains soil height carbon back additive.
5. soil height carbon back additive according to claim 4, is characterized in that, described mixture be by weight percentage 1 ~ 10% add to stalk heap in.
6. soil height carbon back additive according to claim 4, is characterized in that, described mixture be by weight percentage 2 ~ 5% add to stalk heap in.
7. soil height carbon back additive according to claim 4, is characterized in that, regulates heap body water-content to be 50 ~ 65%.
8. soil height carbon back additive according to claim 4, is characterized in that, the condition of described fermentation is that normal temperature fermentation carried out turning after 2 ~ 3 days; Then turning in every 10 ~ 15 days 1 time, altogether turning 2 times; Namely soil height carbon back additive is obtained after last turning completes.
9. soil height carbon back additive according to claim 4, is characterized in that, described composite fungus agent is 1:5 ~ 10 with the mixing quality ratio of wheat-flour.
CN201510059816.6A 2015-02-04 2015-02-04 The high carbon-based additive of soil that a kind of fermented maize stalk is prepared Expired - Fee Related CN104649760B (en)

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CN112226473A (en) * 2020-07-15 2021-01-15 湖北大学 Method for improving enzymolysis efficiency of corn straw by using rhodococcus bifidus
CN117481005A (en) * 2023-09-21 2024-02-02 中国烟草总公司广东省公司 Planting method for combining hillside tobacco and upland rice

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Publication number Priority date Publication date Assignee Title
CN112226473A (en) * 2020-07-15 2021-01-15 湖北大学 Method for improving enzymolysis efficiency of corn straw by using rhodococcus bifidus
CN117481005A (en) * 2023-09-21 2024-02-02 中国烟草总公司广东省公司 Planting method for combining hillside tobacco and upland rice

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