CN104642749A - Composite micro-ecological preparation for promoting animal digestion and absorption - Google Patents
Composite micro-ecological preparation for promoting animal digestion and absorption Download PDFInfo
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- CN104642749A CN104642749A CN201510049374.7A CN201510049374A CN104642749A CN 104642749 A CN104642749 A CN 104642749A CN 201510049374 A CN201510049374 A CN 201510049374A CN 104642749 A CN104642749 A CN 104642749A
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- bacillus subtilis
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- rice bran
- trypsase
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Abstract
The invention discloses a composite micro-ecological preparation for promoting animal digestion and absorption. The composite micro-ecological preparation is prepared from the following raw materials in parts by weight: 3-7 parts of alpha-amylase, 3-7 parts of trypsin, 4-7 parts of catalase, 3-7 parts of superoxide dismutase, 20-40 parts of rice bran, 2*10<8>CFU/g to 2*10<9>CFU/g of viable count of bacillus subtilis, and 100 parts of rice bran; and the composite micro-ecological preparation is prepared by the following preparation steps: a carrying out culture enlarge cultivation on the bacillus subtilis; b adding the rice bran to a bacillus subtilis liquid prepared from the step a, and concentrating into a suspension liquid; and c mixing the suspension liquid obtained from the step b with the alpha-amylase, the trypsin, the catalase and the superoxide dismutase. The micro-ecological preparation provided by the invention is capable of helping animal digestion and absorption, improving the utilization rate of a feed and improving the animal intestinal environment. The selected beneficial bacterium is the bacillus subtilis separated from an animal body, is capable of generating the nutrient substances such as vitamins and enzymes, and can be rapidly planted in the animal body; and the digestion, absorption and utilization rate of the feed can be improved by the amylase and protease.
Description
Technical field
The present invention relates to the compound micro-ecological preparation that a kind of promotion animal digestion absorbs.
Background technology
Bacillus is a kind of aerobic gram-positive bacteria forming gemma, and have resistance to variations in temperature, the fast feature such as resurrection and stronger secretase, microecological bacillus preparation is one of modal probiotics.Bacillus subtilis rapid field planting growth and breeding in animal and bird intestines, consume oxygen unnecessary in enteron aisle, reduce pH and oxidation-reduction potential, create the environment being suitable for the existence of physiological anaerobic bacteria, promote that the useful anaerobic bacterias such as Bifidobacterium grow, recover microecological balance, suppress some harmful pathogenic bacteria or conditioned pathogen simultaneously, regulating intestinal canal microecological balance, makes the bacterium colony integrated compensation of lacking of proper care because of disease to normal condition thus reaches the object for the treatment of and prevention of intestinal tract disease.Bacillus subtilis can secrete the multiple enzymes such as highly active protease, maltose, lipase, amylase, B-dextranase, phytase and cellulase, some more complicated carbohydrate in energy degrading plant feed, supplement the deficiency of animal endogenous enzymes, bacillus subtilis can synthesize the multiple nutrients materials such as multivitamin, protein, amino acid, polypeptide, somatomedin in GI metabolic process, improves digestibility and the utilization rate of protein, starch, cellulose, calcium, phosphorus in feed.
Superoxide dismutase (SOD) is antioxidase important in organism, is distributed widely in various organism, as animal, and plant, microorganism etc.SOD has special physiologically active, is the primary material of scavenging free radicals in organism.SOD level height in vivo means index directly perceived that is old and feeble and death; Now confirm, the disease caused by oxygen radical reaches kind more than 60.It can resist and the infringement blocked because oxygen radical causes cell, and repairs damaged cell in time, restore because of free radical cause to cell damage.Due to modern life pressure, environmental pollution, various radiation and excess motion all can cause oxygen radical to be formed in a large number; Therefore, in biological antioxidant mechanism, the status of SOD is more and more important.Probiotics is more and more welcome in aquaculture, how reasonably to prepare the focus that probiotics becomes research.
Summary of the invention
The present invention is to provide a kind of promotion animal digestion, improves a kind of compound micro-ecological preparation promoting animal digestion to absorb of animal health.
For achieving the above object, the present invention is by the following technical solutions:
Promote the compound micro-ecological preparation that animal digestion absorbs, comprise the raw material of following weight portion: AMS 3-7, trypsase 3-7, catalase 4-7, SOD-3-7, the content 2 × 10 of bacillus subtilis viable count
8-2 × 10
9cFU/g, rice bran 100, described AMS 30000-70000u/g, trypsase 30000-70000u/g, SOD-3 0000-70000u/g, catalase 40000-70000u/g.
The content 2 × 10 of AMS 5, trypsase 5, catalase 4, superoxide dismutase 5, rice bran 35, bacillus subtilis viable count
9cFU/g.
Promote the preparation method of the probiotics that animal digestion absorbs, comprise the steps:
A first by PDA culture medium at 120-125 DEG C, 1.0 × 10
5-1.1 × 10
5under Pa condition, steam sterilizing 20-25 min, then carries out shaking table cultivation by Bacillus subtilis strain in PDA culture medium, is cultured to exponential phase 2 × 10
8-2 × 10
9during CFU/g only, the inoculum concentration of bacillus subtilis is the 1-3% of fluid nutrient medium volume, fermentation temperature 36 DEG C-37 DEG C, fermentation time 2-3 days, fermentation tank pressure 0.01-0.03MPa, fermentation throughput 1: (1-3), fermentation speed of agitator: 150-155 rev/min, pH value 7-7.5;
B adds rice bran and carries out concentrate drying process in the bacillus subtilis bacterium liquid that step a is obtained, is prepared into the suspension that solid content is 15%-25%;
The suspension that step b obtains by c mixes with AMS, trypsase, catalase, superoxide dismutase.
In the present invention, bacillus subtilis is through liquid fermentation, take rice bran as carrier, mixes in proportion after drying is concentrated with AMS, trypsase, superoxide dismutase.Be separated in animal body based on bacillus subtilis, can field planting in animal body rapidly, improve gastrointestinal tract environment, gastrointestinal tract environment can be improved as beneficial bacterium, growth of animal desired nutritional material is provided, and colonization ability is strong in animal body, bacillus subtilis can also secrete digestive enzymes, then supplements amylase and protease, fully supplements the deficiency of body endogenous enzyme; Superoxide dismutase can not only improve immunity of organisms as the nospecific immunity factor, and can improve animal and digest and absorb rate to feed, transfer factor can improve animal immunizing power.Blended to beneficial bacterium, digestive enzymes and the nonspecific immune factors animal digestion that can help absorbs by the present invention, strengthens animal body resistance, prevent disease, saves aquaculture cost.
Detailed description of the invention
embodiment 1
Promote the compound micro-ecological preparation that animal digestion absorbs, comprise the raw material of following weight portion: AMS 3-7, trypsase 3-7, catalase 4-7, SOD-3-7, the content 2 × 10 of bacillus subtilis viable count
8-2 × 10
9cFU/g, rice bran 100, described AMS 30000-70000u/g, trypsase 30000-70000u/g, SOD-3 0000-70000u/g, catalase 40000-70000u/g.
The content 2 × 10 of AMS 5, trypsase 5, catalase 4, superoxide dismutase 5, rice bran 35, bacillus subtilis viable count
9cFU/g.
Promote the preparation method of the probiotics that animal digestion absorbs, comprise the steps:
A first by PDA culture medium at 120-125 DEG C, 1.0 × 10
5-1.1 × 10
5under Pa condition, steam sterilizing 20-25 min, then carries out shaking table cultivation by Bacillus subtilis strain in PDA culture medium, is cultured to exponential phase 2 × 10
8-2 × 10
9during CFU/g only, the inoculum concentration of bacillus subtilis is the 1-3% of fluid nutrient medium volume, fermentation temperature 36 DEG C-37 DEG C, fermentation time 2-3 days, fermentation tank pressure 0.01-0.03MPa, fermentation throughput 1: (1-3), fermentation speed of agitator: 150-155 rev/min, pH value 7-7.5;
B adds rice bran and carries out concentrate drying process in the bacillus subtilis bacterium liquid that step a is obtained, is prepared into the suspension that solid content is 15%-25%;
The suspension that step b obtains by c mixes with AMS, trypsase, catalase, superoxide dismutase.
During use, by 0.1%, product of the present invention is added in animal feed, be applicable to all livestock and poultry and use, monthly use one week continuously.
Embodiment 2
Promote the compound micro-ecological preparation that animal digestion absorbs, in the rice bran of 100 kg, comprise the raw material of following weight: the content 2 × 10 of AMS 5kg, trypsase 5kg, catalase 4kg, superoxide dismutase 5kg, bacillus subtilis viable count
9cFU/g.Described AMS 30000-70000u/g, trypsase 30000-70000u/g, SOD-3 0000-70000u/g, catalase 40000-70000u/g.
Promote the preparation method of the probiotics that animal digestion absorbs, comprise the steps:
A first by PDA culture medium at 121 DEG C, 1.0 × 10
5under Pa condition, steam sterilizing 20 min, then carries out shaking table cultivation by Bacillus subtilis strain in PDA culture medium, is cultured to exponential phase 2 × 10
9during CFU/g only, the inoculum concentration of bacillus subtilis be fluid nutrient medium volume 3%, fermentation temperature 36 DEG C, fermentation time 2 days, fermentation tank pressure 0.01MPa, fermentation throughput 1: 1, fermentation speed of agitator: 150 revs/min, pH value 7;
B adds rice bran and carries out concentrate drying process in the bacillus subtilis bacterium liquid that step a is obtained, is prepared into the suspension that solid content is 20%;
The suspension that step b obtains by c mixes with AMS, trypsase, catalase, superoxide dismutase.
During use, by 0.2%, product of the present invention is added in animal feed, be applicable to all livestock and poultry and use, monthly use one week continuously.
Embodiment 3
Promote the compound micro-ecological preparation that animal digestion absorbs, in the rice bran of 100 kg, comprise the raw material of following weight: the content 2 × 10 of AMS 5kg, trypsase 4kg, catalase 7kg, superoxide dismutase 6kg, bacillus subtilis viable count
9cFU/g.Described AMS 30000-70000u/g, trypsase 30000-70000u/g, SOD-3 0000-70000u/g, catalase 40000-70000u/g.
Promote the preparation method of the probiotics that animal digestion absorbs, comprise the steps:
A first by PDA culture medium at 121 DEG C, 1.0 × 10
5under Pa condition, steam sterilizing 20 min, then carries out shaking table cultivation by Bacillus subtilis strain in PDA culture medium, is cultured to exponential phase 2 × 10
9during CFU/g only, the inoculum concentration of bacillus subtilis be fluid nutrient medium 3%, fermentation temperature 36 DEG C, fermentation time 2 days, fermentation tank pressure 0.01MPa, fermentation throughput 1: 1, fermentation speed of agitator: 150 revs/min, pH value 7;
B adds rice bran and carries out concentrate drying process in the bacillus subtilis bacterium liquid that step a is obtained, is prepared into the suspension that solid content is 20%;
The suspension that step b obtains by c mixes with AMS, trypsase, catalase, superoxide dismutase.
During use, by 0.2%, product of the present invention is added in animal feed, be applicable to all livestock and poultry and use, monthly use one week continuously.
Embodiment 4-18
Detailed description of the invention is as embodiment 2, and composition and the technological parameter of raw material are as shown in table 1:
clinical testing
Select the normal piggy of health 120 with criticizing, the principle close according to body weight and sex is consistent, piggy is divided into A, B, C tri-groups, wherein A group is control group, if 3 repetitions, B test group, if 3 repetitions, often organize 15, male and female half and half, A to feed ordinary maize-soybean meal based diets according to group, and B group adds embodiment 2 product of 0.1% on the basis of A group basal diet.Personal management, two groups give identical feeding and management and environmental condition, Manual material feeding, free choice feeding, freely drink water.
Test period totally 37 days, wherein 7 days preliminary trial periods, 30 days positive experimental periods.
Record each repetition feed intake every day, record each repetition every day diarrhea of pigs head number, dead head number, test is just respectively weighed at end.
Result is as shown in table 2.
In sum, add in little swine rations that product of the present invention can promote that pig grows, improves food conversion ratio, reduces diarrhea rate, there is obvious effect the aspect such as to increase economic efficiency.
Claims (3)
1. the compound micro-ecological preparation promoting animal digestion to absorb, it is characterized in that, comprise the raw material of following weight portion: AMS 3-7, trypsase 3-7, catalase 4-7, SOD-3-7, the content 2 × 10 of bacillus subtilis viable count
8-2 × 10
9cFU/g, rice bran 100, described AMS 30000-70000u/g, trypsase 30000-70000u/g, SOD-3 0000-70000u/g, catalase 40000-70000u/g.
2. the probiotics promoting animal digestion absorption as claimed in claim 1, it is characterized in that, comprise the raw material of following weight portion: the content 2 × 10 of AMS 5, trypsase 5, catalase 4, superoxide dismutase 5, rice bran 35, bacillus subtilis viable count
9cFU/g.
3. the preparation method promoting the probiotics that animal digestion absorbs as claimed in claim 1 or 2, is characterized in that comprising the steps:
A first by PDA culture medium at 120-125 DEG C, 1.0 × 10
5-1.1 × 10
5under Pa condition, steam sterilizing 20-25 min, then carries out shaking table cultivation by Bacillus subtilis strain in PDA culture medium, is cultured to exponential phase 2 × 10
8-2 × 10
9during CFU/g only, the inoculum concentration of bacillus subtilis is the 1-3% of fluid nutrient medium volume, fermentation temperature 36 DEG C-37 DEG C, fermentation time 2-3 days, fermentation tank pressure 0.01-0.03MPa, fermentation throughput 1: (1-3), fermentation speed of agitator: 150-155 rev/min, pH value 7-7.5;
B adds rice bran and carries out concentrate drying process in the bacillus subtilis bacterium liquid that step a is obtained, is prepared into the suspension that solid content is 15%-25%;
The suspension that step b obtains by c mixes with AMS, trypsase, catalase, superoxide dismutase.
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Cited By (5)
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| CN107058181A (en) * | 2017-04-13 | 2017-08-18 | 中国农业科学院特产研究所 | A kind of bacillus subtilis and its separation method and application |
| CN108935323A (en) * | 2018-08-07 | 2018-12-07 | 南宁学院 | A kind of beef cattle breeding method improving efficiency of feed utilization |
| WO2020200322A1 (en) * | 2019-04-05 | 2020-10-08 | Novozymes A/S | Redox enzymes in animal feed compositions |
| WO2020200321A1 (en) * | 2019-04-05 | 2020-10-08 | Novozymes A/S | Redox enzymes in animal feed compositions |
| WO2022074170A1 (en) * | 2020-10-07 | 2022-04-14 | Novozymes A/S | Enzymatic preservation of probiotics in animal feed |
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Cited By (10)
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| WO2020200322A1 (en) * | 2019-04-05 | 2020-10-08 | Novozymes A/S | Redox enzymes in animal feed compositions |
| WO2020200321A1 (en) * | 2019-04-05 | 2020-10-08 | Novozymes A/S | Redox enzymes in animal feed compositions |
| CN113825406A (en) * | 2019-04-05 | 2021-12-21 | 诺维信公司 | Oxidoreductase in animal feed compositions |
| CN113840917A (en) * | 2019-04-05 | 2021-12-24 | 诺维信公司 | Oxidoreductase in animal feed compositions |
| US20220218001A1 (en) * | 2019-04-05 | 2022-07-14 | Novozymes A/S | Redox enzymes in animal feed compositions |
| EP3945844A4 (en) * | 2019-04-05 | 2023-11-22 | Novozymes A/S | Redox enzymes in animal feed compositions |
| WO2022074170A1 (en) * | 2020-10-07 | 2022-04-14 | Novozymes A/S | Enzymatic preservation of probiotics in animal feed |
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