CN104623673A - Preparation method of composite hollow microspheres - Google Patents
Preparation method of composite hollow microspheres Download PDFInfo
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- CN104623673A CN104623673A CN201410750137.9A CN201410750137A CN104623673A CN 104623673 A CN104623673 A CN 104623673A CN 201410750137 A CN201410750137 A CN 201410750137A CN 104623673 A CN104623673 A CN 104623673A
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- gelatin
- microsphere
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- hollow microspheres
- pah
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Abstract
The invention discloses a preparation method of composite hollow microspheres, which belongs to the field of drug carrier preparation. The invention provides a method for preparing composite hollow microspheres by taking a gelatin microsphere as a template. According to the invention, by taking a gelatin microsphere as a template, a gelatin/Si02 / PAH (PSS) composite microsphere is prepared by using a layer-by-layer self-assembling technology, and the template is dissolved by using hot water so as to obtain a Si02 / PAH (PSS) composite hollow microsphere. The method is implemented by taking gelatin as a carrier, because the gelatin has good biocompatibility, biodegradability and no toxicity, even if the template is not completely removed, no negative impact is brought to later drug-loading applications; and the hollow microsphere prepared by using the gelatin template can be controlled in a few hundred nanometers, therefore, the method has important significance to the design of nano drug carriers.
Description
Technical field
The invention belongs to pharmaceutical carrier preparation field.
Background technology
Nano-medicament carrier has height targeting, drug controlled release, the advantages such as the dissolution rate of raising insoluble medicine and absorbance, demonstrate in biological and field of medicaments in recent years widely application prospect (vii) Hollow Microspheres as a kind of special pharmaceutical carrier, high because having drug loading, the features such as slow release effect is obvious have caused people to pay close attention to greatly. at present, the method preparing Hollow Microspheres has multiple, mainly contain drop stove technology, xerogel stove technology, degraded mandrel technology and interfacial polymerization techniques, a kind of brand-new electrostatic self-assembled technology has been applied to this preparation process of mesopore of preparing of Hollow Microspheres in recent years take colloid micro ball as template, after effective surface modification is carried out to template, in template, alternately assembling has the material of opposite charges, prepare complex microsphere, then calcine or obtain Hollow Microspheres by dissolution with solvents removing template.
At present, be that the compound of template and the preparation of Hollow Microspheres have bibliographical information with polystyrene microsphere, melamine resin microsphere, cell, various enzyme and silicon dioxide microsphere, in principle, generally can assemble with the material of electric charge, the material being commonly used to assemble has the polyelectrolyte such as poly (sodium 4-styrenesulfonate) (PSS), PAH (PAH), diallyl dimethyl ammoniumchloride (PDADMAC), polymine (PEI), and Si0
2and Fe
30
4deng inorganic matter nanoparticle.
Summary of the invention
The object of this invention is to provide a kind of take gelatine microsphere as the method for Template preparation Composite Hollow Microspheres.
The present invention is achieved by the following technical programs: a kind of Composite Hollow Microspheres preparation method, it is gelatin solution preheating in the water-bath of 55 DEG C of 5% by weight ratio, be quickly poured into the acetone of equivalent volumes, stratification, remove the turbid liquid of white and the float on upper strata, remaining part is dissolved again in a water bath, adjust ph to 3, drips acetone soln to liquid is creamy white, by magnetic stirrer, add the glutaraldehyde solidification of 8%, obtain gelatine microsphere; Get a certain amount of scattered gelatine microsphere suspension, adsorb poly (sodium 4-styrenesulfonate) successively, PAH solution, be used for carrying out surface modification, make it with more surface charge. then add appropriate nano Si 0
2suspension, constant temperature oscillator adsorbs 0.5h, the Si0 that centrifugal segregation is unnecessary
2particle, scattered in ultrasonic disperser, then centrifuge washing three times, similar step also for adsorbing PAH hydrochloric acid, then alternating sorbent Si0
2with PAH hydrochloric acid, until required thickness, finally, at its surperficial alternating sorbent poly (sodium 4-styrenesulfonate)/polyallyl amine salt; Get complex microsphere suspension, adjust ph is 3, is placed in 60 DEG C of water-baths, and dissolve gelatine microsphere, centrifugal segregation gelatin, obtains Composite Hollow Microspheres.
The present invention has following beneficial effect:
This preparation method adopts gelatin as template, and gelatin has good biocompatibility, degradability and avirulence, even if the non-Ex-all of template, also can not bring negative impact to the medicine carrying application in later stage; The Hollow Microspheres that common various Template preparation go out is mostly at micron order, and the Hollow Microspheres gone out with gelatin Template preparation is smaller comparatively speaking, can be controlled in hundreds of nanometer, significant to design nano-medicament carrier.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention will be further described.
Specific embodiment: preparation process of the present invention is, it is gelatin solution preheating in the water-bath of 55 DEG C of 5% by weight ratio, be quickly poured into the acetone of equivalent volumes, stratification, remove the turbid liquid of white and the float on upper strata, remaining part is dissolved again in a water bath, adjust ph to 3, drips acetone soln to liquid is creamy white, by magnetic stirrer, add the glutaraldehyde solidification of 8%, obtain gelatine microsphere; Get a certain amount of scattered gelatine microsphere suspension, adsorb poly (sodium 4-styrenesulfonate) successively, PAH solution, be used for carrying out surface modification, make it with more surface charge. then add appropriate nano Si 0
2suspension, constant temperature oscillator adsorbs 0.5h, the Si0 that centrifugal segregation is unnecessary
2particle, scattered in ultrasonic disperser, then centrifuge washing three times, similar step also for adsorbing PAH hydrochloric acid, then alternating sorbent Si0
2with PAH hydrochloric acid, until required thickness, finally, at its surperficial alternating sorbent poly (sodium 4-styrenesulfonate)/polyallyl amine salt; Get complex microsphere suspension, adjust ph is 3, is placed in 60 DEG C of water-baths, and dissolve gelatine microsphere, centrifugal segregation gelatin, obtains Composite Hollow Microspheres.
This preparation method take gelatin as Template preparation Composite Hollow Microspheres, and gelatin has good biocompatibility, degradability and avirulence, even if the non-Ex-all of template, also can not bring negative impact to the medicine carrying application in later stage; The Hollow Microspheres that common various Template preparation go out is mostly at micron order, and the Hollow Microspheres gone out with gelatin Template preparation is smaller comparatively speaking, can be controlled in hundreds of nanometer, significant to design nano-medicament carrier.
Above content is the further description done the present invention in conjunction with concrete embodiment, can not assert that specific embodiment of the invention is confined to these explanations.For general technical staff of the technical field of the invention, without departing from the inventive concept of the premise, some simple deduction or replace can also be made, all should be considered as belonging to protection scope of the present invention.
Claims (1)
1. a Composite Hollow Microspheres preparation method, it is characterized in that: be gelatin solution preheating in the water-bath of 55 DEG C of 5% by weight ratio, be quickly poured into the acetone of equivalent volumes, stratification, remove the turbid liquid of white and the float on upper strata, remaining part is dissolved again in a water bath, adjust ph to 3, drips acetone soln to liquid is creamy white, by magnetic stirrer, add the glutaraldehyde solidification of 8%, obtain gelatine microsphere; Get a certain amount of scattered gelatine microsphere suspension, adsorb poly (sodium 4-styrenesulfonate) successively, PAH solution, be used for carrying out surface modification, make it with more surface charge. then add appropriate nano Si 0
2suspension, constant temperature oscillator adsorbs 0.5h, the Si0 that centrifugal segregation is unnecessary
2particle, scattered in ultrasonic disperser, then centrifuge washing three times, similar step also for
Absorption PAH hydrochloric acid, then alternating sorbent Si0
2with PAH hydrochloric acid, until required thickness, finally, at its surperficial alternating sorbent poly (sodium 4-styrenesulfonate)/polyallyl amine salt; Get complex microsphere suspension, adjust ph is 3, is placed in 60 DEG C of water-baths, and dissolve gelatine microsphere, centrifugal segregation gelatin, obtains Composite Hollow Microspheres.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410750137.9A CN104623673A (en) | 2014-12-10 | 2014-12-10 | Preparation method of composite hollow microspheres |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410750137.9A CN104623673A (en) | 2014-12-10 | 2014-12-10 | Preparation method of composite hollow microspheres |
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| CN104623673A true CN104623673A (en) | 2015-05-20 |
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| CN201410750137.9A Pending CN104623673A (en) | 2014-12-10 | 2014-12-10 | Preparation method of composite hollow microspheres |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107828715A (en) * | 2017-11-15 | 2018-03-23 | 佛山科学技术学院 | A kind of plant root edge cell processing method for being used to deposit nano silicon |
-
2014
- 2014-12-10 CN CN201410750137.9A patent/CN104623673A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107828715A (en) * | 2017-11-15 | 2018-03-23 | 佛山科学技术学院 | A kind of plant root edge cell processing method for being used to deposit nano silicon |
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| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20150520 |
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| WD01 | Invention patent application deemed withdrawn after publication |