CN104611397A - Deer blood peptide extraction method - Google Patents
Deer blood peptide extraction method Download PDFInfo
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- CN104611397A CN104611397A CN201510039907.3A CN201510039907A CN104611397A CN 104611397 A CN104611397 A CN 104611397A CN 201510039907 A CN201510039907 A CN 201510039907A CN 104611397 A CN104611397 A CN 104611397A
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Abstract
The invention relates to a deer blood peptide extraction method which comprises the following steps: washing, boiling, extracting decoction, hydrolyzing, filtering, concentrating and performing spray drying. By adopting the deer blood peptide extraction method, the problem of effectively absorbing and utilizing macromolecular protein by human bodies is effectively solved, and macromolecules of protein can be converted and extracted. The deer blood peptide provided by the invention is prepared by extracting animal protein in a pure natural biological enzymolysis manner, is free of chemical component or hormone, free of toxic or side effect on human bodies, and is a very pure nutrient substance. The deer blood peptide can be rapidly dissolved in water without stirring, the decoction can be clear and transparent and is a white or light yellow transparent liquid, and no impurities or precipitates is separated out.
Description
Technical field
The present invention relates to bioengineering field, particularly a kind of method extracting small-molecular peptides from deer blood.
Background technology
In deer blood, organism accounts for 16% ~ 17%, wherein mainly protein, is rich in 19 seed amino acids and multiple enzyme in protein, in addition also containing multiple lipid, free acid kind, steroid, phospholipid, hormones, vitamins and polyose etc.Ash content accounts for 3% ~ 4%, and containing multiple constant and healthy trace elements with household.Particularly in deer blood also containing Y-sphaeroprotein, Gelucystine, Methionin, the creatine phosphokinase relevant to heart body, cover enzyme etc.
At present deer blood products is on the market mostly deer blood meal, air-dry being used as medicine directly drink into wine or blood, and can not change the macromolecular structure of original protein in essence, such product is difficult to be absorbed and utilized by the body as a kind of macromolecular cpd.
Summary of the invention
In order to solve in prior art the problem how allowing high molecular weight protein how effectively be absorbed by human body and to utilize, micromolecular conversion and extraction being carried out to albumen, the invention provides a kind of deer hemepeptide extracting method.Described technical scheme is as follows:
A kind of deer hemepeptide extracting method, comprises the following steps:
Cleaning step, cleans deer blood according to setting cleaning duration, forms the deer blood after cleaning;
Boiling step, water is put into steaming plant, the heavy described deer blood of meter is put into while heating up, and synchronously stir, stop heating up after being warming up to setting heat-preserving range, and be incubated according to setting insulation duration, insulation terminates rear stopping and stirring, then leave standstill duration according to setting to leave standstill, form a soup;
Row's soup step, utilizes draw-out device to extract a described soup, and pumps into a soup storing unit;
Hydrolysing step, a soup in a described soup storing unit is pumped in hydrolysis device, be warming up to setting sterilising temp and keep according to setting sterilizing duration, then be cooled to setting hydrolysis temperature, add configuration liquid and a soup pH value is adjusted to neutrality, drop into animal proteinum zymin and be hydrolyzed, and be retained to setting enzymolysis duration, be warming up to setting enzyme-removal temperature after enzymolysis terminates, and be retained to setting and go out enzyme duration, form the hydrolyzed solution after the enzyme that goes out;
Filtration step, leaves standstill after the hydrolyzed solution after the described enzyme that goes out being dropped into diatomite and/or gac, and utilizes filtration devices to clear liquid storing unit, is then utilized by described clear liquid circulation filter to carry out circulating filtration according to setting circulating filtration duration;
Enrichment step, is concentrated into the concentrated concentration of setting, forms the concentrated solution after clarification by the clear liquid after described circulating filtration;
Spray drying step, carries out spraying dry by the concentrated solution after described clarification according to setting inlet temperature and setting air outlet temperature, forms deer hemepeptide pulvis.
In cleaning step, setting cleaning duration scope is 20 ~ 40 minutes.
In boiling step, setting heat-preserving range is 55 ~ 85 DEG C, and setting insulation duration scope is 0.5 ~ 1.5 hour, and it is 0.5 ~ 1.5 hour that setting leaves standstill duration scope.
In row's soup step, draw-out device is specially vacuum extraction tank.
In hydrolysing step, setting sterilising temp scope is 90 ~ 110 DEG C, setting sterilizing duration scope is 5 ~ 20 minutes, setting hydrolysis temperature scope is 55 ~ 75 DEG C, configuration liquid is specially NaoH, setting enzymolysis duration scope is 4 ~ 6 hours, and setting enzyme-removal temperature scope is 80 ~ 100 DEG C, and setting enzyme duration scope of going out is 5 ~ 25 minutes.
In filtration step, filtration unit is specially tub strainer and/or two-stage plate filter, and circulation filter is specially plate filter, and setting circulating filtration duration scope is 1 ~ 3 hour.
In enrichment step, the concentrated concentration range of setting is 10 ~ 20 Baumes, need recycle plate filter and filters or utilize tubular-bowl centrifuge to be separated, the concentrated solution of described muddiness is formed the concentrated solution of clarification to the concentrated solution of muddiness.
In spray drying step, setting inlet temperature scope is 190 ~ 210 DEG C, and setting air outlet temperature scope is 90-100 DEG C.
The present invention efficiently solves the problem how high molecular weight protein is effectively absorbed by human body and to utilize, and carries out micromolecular conversion and extraction to albumen.Deer hemepeptide of the present invention is that animal proteinum pure-natural biological enzymolysis and extraction forms, and not containing any chemical composition and hormone, to human body without any toxic side effect, is very pure nutritive substance.Can be water-soluble rapidly without the need to stirring, the aqueous solution is limpid transparent, is white or light yellow transparent liquid, can not separates out any impurity and throw out.
Deer hemepeptide molecular weight, all below 1000 dalton, has the high property digested and assimilated and utilization ratio.It without gastro-intestinal digestion, directly can be absorbed by the body.Effectively can ensure human body supplementing protein.Reasonable supplement small-molecular peptides, can make the abundance keeping nutrition in human body, and each histoorgan of supply health runs well, and maintains form, body is kept fit and vigor.Rich amino acids in small-molecular peptides and not fatty, does not need to digest direct absorption; Biological activity by force can absorbed intact; Uncompetitive active absorption; Consuming energy lowly does not increase stomach burden.Supplementary small-molecular peptides can play obvious regulation of physiological functions, has vital effect to the physiological activity such as growth, growth, metabolism, procreation of human body.
Should be understood that, it is only exemplary that above general description and details hereinafter describe, and can not limit the present invention.
Accompanying drawing explanation
Accompanying drawing to be herein merged in specification sheets and to form the part of this specification sheets, shows embodiment according to the invention, and is used from specification sheets one and explains principle of the present invention.
Fig. 1 is method flow schematic diagram of the present invention;
Fig. 2 is the method flow schematic diagram of the embodiment of the present invention.
Embodiment
Here will be described exemplary embodiment in detail, its sample table shows in the accompanying drawings.When description below relates to accompanying drawing, unless otherwise indicated, the same numbers in different accompanying drawing represents same or analogous key element.Embodiment described in following exemplary embodiment does not represent all embodiments consistent with the present invention.On the contrary, they only with as in appended claims describe in detail, the example of apparatus and method that aspects more of the present invention are consistent.
Fig. 1 is according to method flow schematic diagram of the present invention, relates to a kind of deer hemepeptide extracting method, comprises the following steps:
Cleaning step 101: deer blood is cleaned according to setting cleaning duration, forms the deer blood after cleaning.Setting cleaning duration scope is 20 ~ 40 minutes.
Boiling step 102: water is put into steaming plant, the heavy described deer blood of meter is put into while heating up, and synchronously stir, stop heating up after being warming up to setting heat-preserving range, and be incubated according to setting insulation duration, insulation terminates rear stopping and stirring, and then leaves standstill duration according to setting and leaves standstill, form a soup.Setting heat-preserving range is 55 ~ 85 DEG C, and setting insulation duration scope is 0.5 ~ 1.5 hour, and it is 0.5 ~ 1.5 hour that setting leaves standstill duration scope.
Row's soup step 103: utilize draw-out device to extract a described soup, and pump into a soup storing unit.Draw-out device is specially vacuum extraction tank.
Hydrolysing step 104 a: soup in a described soup storing unit is pumped in hydrolysis device, be warming up to setting sterilising temp and keep according to setting sterilizing duration, then setting hydrolysis temperature is cooled to, add configuration liquid and a soup pH value is adjusted to neutrality, drop into animal proteinum zymin to be hydrolyzed, and be retained to setting enzymolysis duration, after enzymolysis terminates, be warming up to setting enzyme-removal temperature, and be retained to setting and go out enzyme duration, form the hydrolyzed solution after the enzyme that goes out.Setting sterilising temp scope is 90 ~ 110 DEG C, setting sterilizing duration scope is 5 ~ 20 minutes, setting hydrolysis temperature scope is 55 ~ 75 DEG C, configuration liquid is specially NaoH, setting enzymolysis duration scope is 4 ~ 6 hours, setting enzyme-removal temperature scope is 80 ~ 100 DEG C, and setting enzyme duration scope of going out is 5 ~ 25 minutes.
Filtration step 105: leave standstill after the hydrolyzed solution after the described enzyme that goes out being dropped into diatomite and/or gac, and utilize filtration devices to clear liquid storing unit, then utilizes circulation filter to carry out circulating filtration according to setting circulating filtration duration by described clear liquid.Filtration unit is specially tub strainer and/or two-stage plate filter, and circulation filter is specially plate filter, and setting circulating filtration duration scope is 1 ~ 3 hour.
Enrichment step 106: the clear liquid after described circulating filtration is concentrated into the concentrated concentration of setting, forms the concentrated solution after clarification.The concentrated concentration range of setting is 10 ~ 20 Baumes, need recycle plate filter and filters or utilize tubular-bowl centrifuge to be separated, the concentrated solution of described muddiness is formed the concentrated solution of clarification to the concentrated solution of muddiness.
Spray drying step 107: the concentrated solution after described clarification is carried out spraying dry according to setting inlet temperature and setting air outlet temperature, forms deer hemepeptide pulvis.Setting inlet temperature scope is 190 ~ 210 DEG C, and setting air outlet temperature scope is 90-100 DEG C.
Fig. 2 is the method flow schematic diagram of embodiment provided by the invention, relates to a kind of deer hemepeptide extracting method, comprises the following steps:
Cleaning step 201: cleaned according to 30 minutes by deer blood, forms the deer blood after cleaning.
Boiling step 202: water is put into steaming plant, puts into the heavy described deer blood of meter, and synchronously stirs while intensification, stop after being warming up to 65 ~ 75 DEG C heating up, and be incubated according to 1 hour, insulation terminates rear stopping and stirring, then left standstill according to 1 hour, form a soup.
Row's soup step 203: utilize vacuum extraction tank to extract a described soup, and pump into a soup storing unit.
Hydrolysing step 204 a: soup in a described soup storing unit is pumped in hydrolysis device, be warming up to 98 ~ 100 DEG C and kept according to 10 ~ 15 minutes, then 60 ~ 65 DEG C are cooled to, add NaoH and a soup pH value is adjusted to neutrality, drop into animal proteinum zymin to be hydrolyzed, and be retained to 5 hours, after enzymolysis terminates, be warming up to 90 DEG C, and be retained to 15 minutes, form the hydrolyzed solution after the enzyme that goes out.
Filtration step 205: leave standstill after the hydrolyzed solution after the described enzyme that goes out being dropped into diatomite and/or gac, and utilize tub strainer and/or two-stage sheet frame metre filter to clear liquid storing unit, then utilize plate filter to carry out circulating filtration according to 2 hours described clear liquid.
Enrichment step 206: the clear liquid after described circulating filtration is concentrated into 13 ~ 15 Baumes, forms the concentrated solution after clarification.
Spray drying step 207: the concentrated solution after described clarification is carried out spraying dry according to inlet temperature 200 DEG C and air outlet temperature 93 ~ 95 DEG C, forms deer hemepeptide pulvis.
The present invention efficiently solves the problem how high molecular weight protein is effectively absorbed by human body and to utilize, and carries out micromolecular conversion and extraction to albumen.Deer hemepeptide of the present invention is that animal proteinum pure-natural biological enzymolysis and extraction forms, and not containing any chemical composition and hormone, to human body without any toxic side effect, is very pure nutritive substance.Can be water-soluble rapidly without the need to stirring, the aqueous solution is limpid transparent, is white or light yellow transparent liquid, can not separates out any impurity and throw out.
Deer hemepeptide molecular weight, all below 1000 dalton, has the high property digested and assimilated and utilization ratio.It without gastro-intestinal digestion, directly can be absorbed by the body.Effectively can ensure human body supplementing protein.Reasonable supplement small-molecular peptides, can make the abundance keeping nutrition in human body, and each histoorgan of supply health runs well, and maintains form, body is kept fit and vigor.Rich amino acids in small-molecular peptides and not fatty, does not need to digest direct absorption; Biological activity by force can absorbed intact; Uncompetitive active absorption; Consuming energy lowly does not increase stomach burden.Supplementary small-molecular peptides can play obvious regulation of physiological functions, has vital effect to the physiological activity such as growth, growth, metabolism, procreation of human body.
Those skilled in the art, at consideration specification sheets and after putting into practice invention disclosed herein, will easily expect other embodiment of the present invention.The application is intended to contain any modification of the present invention, purposes or adaptations, and these modification, purposes or adaptations are followed general principle of the present invention and comprised the undocumented common practise in the art of the present invention or conventional techniques means.Specification sheets and embodiment are only regarded as exemplary, and true scope of the present invention and spirit are pointed out by claim below.Should be understood that, the present invention is not limited to precision architecture described above and illustrated in the accompanying drawings, and can carry out various amendment and change not departing from its scope.Scope of the present invention is only limited by appended claim.
Claims (10)
1. a deer hemepeptide extracting method, is characterized in that, said method comprising the steps of:
Cleaning step, cleans deer blood according to setting cleaning duration, forms the deer blood after cleaning;
Boiling step, water is put into steaming plant, the heavy described deer blood of meter is put into while heating up, and synchronously stir, stop heating up after being warming up to setting heat-preserving range, and be incubated according to setting insulation duration, insulation terminates rear stopping and stirring, then leave standstill duration according to setting to leave standstill, form a soup;
Row's soup step, utilizes draw-out device to extract a described soup, and pumps into a soup storing unit;
Hydrolysing step, a soup in a described soup storing unit is pumped in hydrolysis device, be warming up to setting sterilising temp and keep according to setting sterilizing duration, then be cooled to setting hydrolysis temperature, add configuration liquid and a soup pH value is adjusted to neutrality, drop into animal proteinum zymin and be hydrolyzed, and be retained to setting enzymolysis duration, be warming up to setting enzyme-removal temperature after enzymolysis terminates, and be retained to setting and go out enzyme duration, form the hydrolyzed solution after the enzyme that goes out;
Filtration step, leaves standstill after the hydrolyzed solution after the described enzyme that goes out being dropped into diatomite and/or gac, and utilizes filtration devices to clear liquid storing unit, is then utilized by described clear liquid circulation filter to carry out circulating filtration according to setting circulating filtration duration;
Enrichment step, is concentrated into the concentrated concentration of setting, forms the concentrated solution after clarification by the clear liquid after described circulating filtration;
Spray drying step, carries out spraying dry by the concentrated solution after described clarification according to setting inlet temperature and setting air outlet temperature, forms deer hemepeptide pulvis.
2. a kind of deer hemepeptide extracting method according to claim 1, it is characterized in that, the setting heat-preserving range in described boiling step is 55 ~ 85 DEG C, and setting insulation duration scope is 0.5 ~ 1.5 hour.
3. a kind of deer hemepeptide extracting method according to claim 1, it is characterized in that, it is 0.5 ~ 1.5 hour that the setting in described boiling step leaves standstill duration scope.
4. a kind of deer hemepeptide extracting method according to claim 1, is characterized in that, the setting sterilising temp scope in described hydrolysing step is 90 ~ 110 DEG C, and setting sterilizing duration scope is 5 ~ 20 minutes.
5. a kind of deer hemepeptide extracting method according to claim 1, is characterized in that, the setting hydrolysis temperature scope in described hydrolysing step is 55 ~ 75 DEG C, and setting enzymolysis duration scope is 4 ~ 6 hours.
6. a kind of deer hemepeptide extracting method according to claim 1, is characterized in that, the setting enzyme-removal temperature scope in described hydrolysing step is 80 ~ 100 DEG C, and setting enzyme duration scope of going out is 5 ~ 25 minutes.
7. a kind of deer hemepeptide extracting method according to claim 1, it is characterized in that, the filtration unit in described filtration step is specially tub strainer and/or two-stage plate filter.
8. a kind of deer hemepeptide extracting method according to claim 1, is characterized in that, the setting circulating filtration duration scope in described filtration step is 1 ~ 3 hour.
9. a kind of deer hemepeptide extracting method according to claim 1, it is characterized in that, it is 10 ~ 20 Baumes that the setting in described enrichment step concentrates concentration range.
10. a kind of deer hemepeptide Advanced method according to claim 1, is characterized in that, the setting inlet temperature scope in described spray drying step is 190 ~ 210 DEG C, and setting air outlet temperature scope is 90-100 DEG C.
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| CN201510039907.3A CN104611397A (en) | 2015-01-27 | 2015-01-27 | Deer blood peptide extraction method |
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| CN201510039907.3A CN104611397A (en) | 2015-01-27 | 2015-01-27 | Deer blood peptide extraction method |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108967795A (en) * | 2018-07-04 | 2018-12-11 | 长春金荷药业有限公司 | A kind of deer hemepeptide solid beverage |
| CN111296849A (en) * | 2020-03-24 | 2020-06-19 | 中科花鹿农业发展有限公司 | Anti-aging bird nest snow clam deer blood collagen compound peptide beverage and preparation method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102199648A (en) * | 2011-03-25 | 2011-09-28 | 伊通满族自治县吉云鹿业发展有限公司 | Method for acquiring bioactive peptide by sequentially hydrolyzing deer haemproteins with two enzymes |
| CN102367465A (en) * | 2011-10-18 | 2012-03-07 | 得利斯集团有限公司 | Extraction method of pig blood protein peptide |
| CN103266156A (en) * | 2013-05-07 | 2013-08-28 | 黑龙江省野生动物研究所 | Method for preparing deer blood protein peptide by using fractional enzymolysis technology |
-
2015
- 2015-01-27 CN CN201510039907.3A patent/CN104611397A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102199648A (en) * | 2011-03-25 | 2011-09-28 | 伊通满族自治县吉云鹿业发展有限公司 | Method for acquiring bioactive peptide by sequentially hydrolyzing deer haemproteins with two enzymes |
| CN102367465A (en) * | 2011-10-18 | 2012-03-07 | 得利斯集团有限公司 | Extraction method of pig blood protein peptide |
| CN103266156A (en) * | 2013-05-07 | 2013-08-28 | 黑龙江省野生动物研究所 | Method for preparing deer blood protein peptide by using fractional enzymolysis technology |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108967795A (en) * | 2018-07-04 | 2018-12-11 | 长春金荷药业有限公司 | A kind of deer hemepeptide solid beverage |
| CN111296849A (en) * | 2020-03-24 | 2020-06-19 | 中科花鹿农业发展有限公司 | Anti-aging bird nest snow clam deer blood collagen compound peptide beverage and preparation method thereof |
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Application publication date: 20150513 |