CN104561209A - Extraction method of black-bone chicken peptide - Google Patents
Extraction method of black-bone chicken peptide Download PDFInfo
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- CN104561209A CN104561209A CN201510039899.2A CN201510039899A CN104561209A CN 104561209 A CN104561209 A CN 104561209A CN 201510039899 A CN201510039899 A CN 201510039899A CN 104561209 A CN104561209 A CN 104561209A
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Abstract
The invention relates to an extraction method of a black-bone chicken peptide. The extraction method comprises a washing and crushing step, a cooking step, a soup drainage step, a hydrolysis step, a filtration step, a concentration step and a spray-drying step. By adopting the extraction method provided by the invention, the problem of effective absorption and utilization of macromolecular proteins in a human body is effectively solved, and small molecular conversion and extraction are performed on the proteins. The black-bone chicken peptide is a small molecular peptide type substance formed by performing biological enzymolysis extraction on a black-bone chicken, does not contain chemical components or hormones, has no toxic or side effects to the human body, is a very pure nutritional substance and can be rapidly dissolved in water without stirring, and a water solution is clear and transparent, is white or canary yellow transparent liquid, and cannot precipitate impurities and precipitates. The black-bone chicken peptide can improve physiological functions, delay aging and strengthen tendons and bones, and has obvious effects for preventing and treating osteoporosis, rickets, iron deficiency anemia of women and the like.
Description
Technical field
The present invention relates to bioengineering field, particularly a kind of method extracting small-molecular peptides from black-bone chicken.
Background technology
Black-bone chicken includes abundant melanochrome, protein, vitamin B group etc. 18 seed amino acid and 18 kinds of trace elements, and wherein the content of nicotinic acid, vitamin-E, phosphorus, iron, potassium, sodium is all higher than common chicken, and cholesterol and lipid content are but very low.The total serum protein of black-bone chicken and globular protein content are all apparently higher than common chicken.Amino acid is contained higher than common chicken in blackbone Chicken, and also higher than common chicken a lot of containing ferro element, be the invigorant of high nutritive value.Edible black-bone chicken can be improved physiological function, delayed senility, strengthening the muscles and bones.Obvious effect is had to preventing and treating osteoporosis, rickets, women's iron deficiency anemia etc.
The purposes of current black-bone chicken mostly on the market is direct heating and eats or be used as medicine, and effective constituent wherein can not play completely and utilize, can not change the macromolecular structure of original protein in essence, traditional using method expends the one of black-bone chicken effective constituent.
Summary of the invention
In order to solve in prior art the problem how allowing high molecular weight protein how effectively be absorbed by human body and to utilize, micromolecular conversion and extraction being carried out to albumen, the invention provides a kind of black-bone chicken peptide extracting method.Described technical scheme is as follows:
A kind of black-bone chicken peptide extracting method, comprises the following steps:
Cleaning destruction step, cleans black-bone chicken according to setting cleaning duration, and the black-bone chicken after cleaning is carried out fragmentation according to the broken size of setting;
Boiling step, water is put into steaming plant, the heavy described black-bone chicken of meter is put into while heating up, and synchronously stir, stop heating up after being warming up to setting heat-preserving range, and be incubated according to setting insulation duration, insulation terminates rear stopping and stirring, then leave standstill duration according to setting to leave standstill, form a soup;
Row's soup step, utilizes draw-out device to extract a described soup, and pumps into a soup storing unit;
Hydrolysing step, a soup in a described soup storing unit is pumped in hydrolysis device, be warming up to setting sterilising temp and keep according to setting sterilizing duration, then be cooled to setting hydrolysis temperature, add configuration liquid and a soup pH value is adjusted to neutrality, drop into animal proteinum zymin and be hydrolyzed, and be retained to setting enzymolysis duration, be warming up to setting enzyme-removal temperature after enzymolysis terminates, and be retained to setting and go out enzyme duration, form the hydrolyzed solution after the enzyme that goes out;
Filtration step, leaves standstill after the hydrolyzed solution after the described enzyme that goes out being dropped into diatomite and/or gac, and utilizes filtration devices to clear liquid storing unit, is then utilized by described clear liquid circulation filter to carry out circulating filtration according to setting circulating filtration duration;
Enrichment step, is concentrated into the concentrated concentration of setting, forms the concentrated solution after clarification by the clear liquid after described circulating filtration;
Spray drying step, carries out spraying dry by the concentrated solution after described clarification according to setting inlet temperature and setting air outlet temperature, forms black-bone chicken peptide pulvis.
In cleaning destruction step, setting cleaning duration scope is 20 ~ 40 minutes, and setting broken size range is 5 ~ 15 centimetres.
In boiling step, setting heat-preserving range is 50 ~ 70 DEG C, and setting insulation duration scope is 0.5 ~ 1.5 hour, and it is 0.5 ~ 1.5 hour that setting leaves standstill duration scope.
In row's soup step, draw-out device is specially vacuum extraction tank.
In hydrolysing step, setting sterilising temp scope is 70 ~ 90 DEG C, setting sterilizing duration scope is 5 ~ 20 minutes, setting hydrolysis temperature scope is 55 ~ 75 DEG C, configuration liquid is specially NaoH, setting enzymolysis duration scope is 0.5 ~ 1.5 hour, and setting enzyme-removal temperature scope is 70 ~ 90 DEG C, and setting enzyme duration scope of going out is 5 ~ 20 minutes.
In filtration step, filtration unit is specially tub strainer and/or two-stage plate filter, and circulation filter is specially plate filter, and setting circulating filtration duration scope is 0.5 ~ 1.5 hour.
In enrichment step, the concentrated concentration range of setting is 10 ~ 20 Baumes, need recycle plate filter and filters or utilize tubular-bowl centrifuge to be separated, the concentrated solution of described muddiness is formed the concentrated solution of clarification to the concentrated solution of muddiness.
In spray drying step, setting inlet temperature scope is 140 ~ 160 DEG C, and setting air outlet temperature scope is 90-100 DEG C.
The present invention efficiently solves the problem how high molecular weight protein is effectively absorbed by human body and to utilize, and carries out micromolecular conversion and extraction to albumen.Black-bone chicken peptide of the present invention carries out biological enzymolysis extraction to black-bone chicken to form small-molecule peptide material, not containing any chemical composition and hormone, to human body without any toxic side effect, is very pure nutritive substance.Can be water-soluble rapidly without the need to stirring, the aqueous solution is limpid transparent, is white or light yellow transparent liquid, can not separates out any impurity and throw out.Physiological function can be improved, delay senility, strengthening the muscles and bones.Obvious effect is had to preventing and treating osteoporosis, rickets, women's iron deficiency anemia etc.
Small active peptides is made after the extraction of special proteasome degradation by fresh for black-bone chicken kindred, and its molecular weight, all below 1000 dalton, has the high property digested and assimilated and utilization ratio.It without gastro-intestinal digestion, directly can be absorbed by the body.Effectively can ensure human body supplementing protein.
Black-bone chicken peptide can improve physiological function, delays senility, strengthening the muscles and bones.Obvious effect is had to preventing and treating osteoporosis, rickets, women's iron deficiency anemia etc.Reasonable supplement black-bone chicken peptide, can make the abundance keeping nutrition in human body, and each histoorgan of supply health runs well, and maintains form, body is kept fit and vigor.Rich amino acids in black-bone chicken peptide and not fatty, does not need to digest direct absorption; Biological activity by force can absorbed intact; Uncompetitive active absorption; Consuming energy lowly does not increase stomach burden.
Should be understood that, it is only exemplary that above general description and details hereinafter describe, and can not limit the present invention.
Accompanying drawing explanation
Accompanying drawing to be herein merged in specification sheets and to form the part of this specification sheets, shows embodiment according to the invention, and is used from specification sheets one and explains principle of the present invention.
Fig. 1 is method flow schematic diagram of the present invention;
Fig. 2 is the method flow schematic diagram of the embodiment of the present invention.
Embodiment
Here will be described exemplary embodiment in detail, its sample table shows in the accompanying drawings.When description below relates to accompanying drawing, unless otherwise indicated, the same numbers in different accompanying drawing represents same or analogous key element.Embodiment described in following exemplary embodiment does not represent all embodiments consistent with the present invention.On the contrary, they only with as in appended claims describe in detail, the example of apparatus and method that aspects more of the present invention are consistent.
Fig. 1 is according to method flow schematic diagram of the present invention, relates to a kind of black-bone chicken peptide extracting method, comprises the following steps:
Cleaning destruction step 101: cleaned according to setting cleaning duration by black-bone chicken, carries out fragmentation by the black-bone chicken after cleaning according to the broken size of setting.Setting cleaning duration scope is 20 ~ 40 minutes, and setting broken size range is 5 ~ 15 centimetres.
Boiling step 102: water is put into steaming plant, the heavy described black-bone chicken of meter is put into while heating up, and synchronously stir, stop heating up after being warming up to setting heat-preserving range, and be incubated according to setting insulation duration, insulation terminates rear stopping and stirring, and then leaves standstill duration according to setting and leaves standstill, form a soup.Setting heat-preserving range is 50 ~ 70 DEG C, and setting insulation duration scope is 0.5 ~ 1.5 hour, and it is 0.5 ~ 1.5 hour that setting leaves standstill duration scope.
Row's soup step 103: utilize draw-out device to extract a described soup, and pump into a soup storing unit.Draw-out device is specially vacuum extraction tank.
Hydrolysing step 104 a: soup in a described soup storing unit is pumped in hydrolysis device, be warming up to setting sterilising temp and keep according to setting sterilizing duration, then setting hydrolysis temperature is cooled to, add configuration liquid and a soup pH value is adjusted to neutrality, drop into animal proteinum zymin to be hydrolyzed, and be retained to setting enzymolysis duration, after enzymolysis terminates, be warming up to setting enzyme-removal temperature, and be retained to setting and go out enzyme duration, form the hydrolyzed solution after the enzyme that goes out.Setting sterilising temp scope is 70 ~ 90 DEG C, setting sterilizing duration scope is 5 ~ 20 minutes, setting hydrolysis temperature scope is 55 ~ 75 DEG C, configuration liquid is specially NaoH, setting enzymolysis duration scope is 0.5 ~ 1.5 hour, setting enzyme-removal temperature scope is 70 ~ 90 DEG C, and setting enzyme duration scope of going out is 5 ~ 20 minutes.
Filtration step 105: leave standstill after the hydrolyzed solution after the described enzyme that goes out being dropped into diatomite and/or gac, and utilize filtration devices to clear liquid storing unit, then utilizes circulation filter to carry out circulating filtration according to setting circulating filtration duration by described clear liquid.Filtration unit is specially tub strainer and/or two-stage plate filter, and circulation filter is specially plate filter, and setting circulating filtration duration scope is 0.5 ~ 1.5 hour.
Enrichment step 106: the clear liquid after described circulating filtration is concentrated into the concentrated concentration of setting, forms the concentrated solution after clarification.The concentrated concentration range of setting is 10 ~ 20 Baumes, need recycle plate filter and filters or utilize tubular-bowl centrifuge to be separated, the concentrated solution of described muddiness is formed the concentrated solution of clarification to the concentrated solution of muddiness.
Spray drying step 107: the concentrated solution after described clarification is carried out spraying dry according to setting inlet temperature and setting air outlet temperature, forms black-bone chicken peptide pulvis.Setting inlet temperature scope is 140 ~ 160 DEG C, and setting air outlet temperature scope is 90-100 DEG C.
Fig. 2 is the method flow schematic diagram of embodiment provided by the invention, relates to a kind of black-bone chicken peptide extracting method, comprises the following steps:
Cleaning destruction step 201: cleaned according to 30 minutes by black-bone chicken, carries out fragmentation by the black-bone chicken after cleaning according to 10 centimetres.
Boiling step 202: water is put into steaming plant, puts into the heavy described black-bone chicken of meter, and synchronously stirs while intensification, stop after being warming up to 55 ~ 65 DEG C heating up, and be incubated according to 1 hour, insulation terminates rear stopping and stirring, then left standstill according to 1 hour, form a soup.
Row's soup step 203: utilize vacuum extraction tank to extract a described soup, and pump into a soup storing unit.
Hydrolysing step 204 a: soup in a described soup storing unit is pumped in hydrolysis device, be warming up to 80 DEG C and kept according to 15 minutes, then 60 ~ 65 DEG C are cooled to, add NaoH and a soup pH value is adjusted to neutrality, drop into animal proteinum zymin to be hydrolyzed, and be retained to 1 hour, after enzymolysis terminates, be warming up to 80 DEG C, and be retained to 15 minutes, form the hydrolyzed solution after the enzyme that goes out.
Filtration step 205: leave standstill after the hydrolyzed solution after the described enzyme that goes out being dropped into diatomite and/or gac, and utilize tub strainer and/or two-stage sheet frame metre filter to clear liquid storing unit, then utilize plate filter to carry out circulating filtration according to 1 hour described clear liquid.
Enrichment step 206: the clear liquid after described circulating filtration is concentrated into 13 ~ 15 Baumes, forms the concentrated solution after clarification.
Spray drying step 207: the concentrated solution after described clarification is carried out spraying dry according to inlet temperature 150 DEG C and air outlet temperature 90 ~ 95 DEG C, forms black-bone chicken peptide pulvis.
The present invention efficiently solves the problem how high molecular weight protein is effectively absorbed by human body and to utilize, and carries out micromolecular conversion and extraction to albumen.Black-bone chicken peptide of the present invention carries out biological enzymolysis to black-bone chicken to extract and form small-molecule peptide material, and the essential ingredient activity in black-bone chicken peptide is superpower, is very easily absorbed by the body, can adjusting immunity of human body comprehensively.The present invention containing any chemical composition and hormone, to human body without any toxic side effect, is not very pure nutritive substance.Can be water-soluble rapidly without the need to stirring, the aqueous solution is limpid transparent, is white or light yellow transparent liquid, can not separates out any impurity and throw out.
Its molecular weight of black-bone chicken peptide, all below 1000 dalton, has the high property digested and assimilated and utilization ratio.It without gastro-intestinal digestion, directly can be absorbed by the body.Cytologic experiments shows, black-bone chicken Toplink effectively prevents the infringement of radical pair human body cell film, the aging course of reducing human cell, can activity that is anti-oxidant, enhancement of SOD effectively.Effectively can improve the content of PGI2 in human body, prevent platelet aggregation, have provide protection to cardiovascular and cerebrovascular.Black-bone chicken peptide has the effect improving hemochrome, prevention anaemia.Animal experiment shows, black-bone chicken peptide has anti-inflammatory, the effect of anti-stain ulcer, has unusual effect to the rehabilitation of stomach stain ulcer and operation patients.Bone growth, prevention children rachitis, senile osteoporosis can be promoted, shorten the convalescence of fracture patient.
Those skilled in the art, at consideration specification sheets and after putting into practice invention disclosed herein, will easily expect other embodiment of the present invention.The application is intended to contain any modification of the present invention, purposes or adaptations, and these modification, purposes or adaptations are followed general principle of the present invention and comprised the undocumented common practise in the art of the present invention or conventional techniques means.Specification sheets and embodiment are only regarded as exemplary, and true scope of the present invention and spirit are pointed out by claim below.Should be understood that, the present invention is not limited to precision architecture described above and illustrated in the accompanying drawings, and can carry out various amendment and change not departing from its scope.Scope of the present invention is only limited by appended claim.
Claims (10)
1. a black-bone chicken peptide extracting method, is characterized in that, said method comprising the steps of:
Cleaning destruction step, cleans black-bone chicken according to setting cleaning duration, and the black-bone chicken after cleaning is carried out fragmentation according to the broken size of setting;
Boiling step, water is put into steaming plant, the heavy described black-bone chicken of meter is put into while heating up, and synchronously stir, stop heating up after being warming up to setting heat-preserving range, and be incubated according to setting insulation duration, insulation terminates rear stopping and stirring, then leave standstill duration according to setting to leave standstill, form a soup;
Row's soup step, utilizes draw-out device to extract a described soup, and pumps into a soup storing unit;
Hydrolysing step, a soup in a described soup storing unit is pumped in hydrolysis device, be warming up to setting sterilising temp and keep according to setting sterilizing duration, then be cooled to setting hydrolysis temperature, add configuration liquid and a soup pH value is adjusted to neutrality, drop into animal proteinum zymin and be hydrolyzed, and be retained to setting enzymolysis duration, be warming up to setting enzyme-removal temperature after enzymolysis terminates, and be retained to setting and go out enzyme duration, form the hydrolyzed solution after the enzyme that goes out;
Filtration step, leaves standstill after the hydrolyzed solution after the described enzyme that goes out being dropped into diatomite and/or gac, and utilizes filtration devices to clear liquid storing unit, is then utilized by described clear liquid circulation filter to carry out circulating filtration according to setting circulating filtration duration;
Enrichment step, is concentrated into the concentrated concentration of setting, forms the concentrated solution after clarification by the clear liquid after described circulating filtration;
Spray drying step, carries out spraying dry by the concentrated solution after described clarification according to setting inlet temperature and setting air outlet temperature, forms black-bone chicken peptide pulvis.
2. a kind of black-bone chicken peptide extracting method according to claim 1, is characterized in that, the broken size range of the setting in described cleaning destruction step is 5 ~ 15 centimetres.
3. a kind of black-bone chicken peptide extracting method according to claim 1, it is characterized in that, the setting heat-preserving range in described boiling step is 50 ~ 70 DEG C, and setting insulation duration scope is 0.5 ~ 1.5 hour.
4. a kind of black-bone chicken peptide extracting method according to claim 1, is characterized in that, the setting sterilising temp scope in described hydrolysing step is 70 ~ 90 DEG C, and setting sterilizing duration scope is 5 ~ 20 minutes.
5. a kind of black-bone chicken peptide extracting method according to claim 1, is characterized in that, the setting hydrolysis temperature scope in described hydrolysing step is 55 ~ 75 DEG C, and setting enzymolysis duration scope is 0.5 ~ 1.5 hour.
6. a kind of black-bone chicken peptide extracting method according to claim 1, it is characterized in that, the configuration liquid in described hydrolysing step is specially NaoH.
7. a kind of black-bone chicken peptide extracting method according to claim 1, is characterized in that, the setting enzyme-removal temperature scope in described hydrolysing step is 70 ~ 90 DEG C, and setting enzyme duration scope of going out is 5 ~ 20 minutes.
8. a kind of black-bone chicken peptide extracting method according to claim 1, it is characterized in that, the filtration unit in described filtration step is specially tub strainer and/or two-stage plate filter.
9. a kind of black-bone chicken peptide extracting method according to claim 1, it is characterized in that, it is 10 ~ 20 Baumes that the setting in described enrichment step concentrates concentration range.
10. a kind of black-bone chicken peptide Advanced method according to claim 1, is characterized in that, the setting inlet temperature scope in described spray drying step is 140 ~ 160 DEG C, and setting air outlet temperature scope is 90-100 DEG C.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106755247A (en) * | 2016-12-13 | 2017-05-31 | 国肽生物科技(北京)有限公司 | The extracting method of crocodile peptide |
| CN108402459A (en) * | 2018-03-06 | 2018-08-17 | 深圳施之健生命科技有限公司 | Snakehead Gallus domesticlus brisson book on Chinese herbal medicine composition and its preparation method and application |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102787154A (en) * | 2011-05-20 | 2012-11-21 | 中国食品发酵工业研究院 | Preparation method of black-bone chicken oligopeptide and separation and identification method of active peptide fragment |
| CN104164466A (en) * | 2014-06-27 | 2014-11-26 | 宗树伟 | A ginseng peptide extraction method |
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2015
- 2015-01-27 CN CN201510039899.2A patent/CN104561209A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102787154A (en) * | 2011-05-20 | 2012-11-21 | 中国食品发酵工业研究院 | Preparation method of black-bone chicken oligopeptide and separation and identification method of active peptide fragment |
| CN104164466A (en) * | 2014-06-27 | 2014-11-26 | 宗树伟 | A ginseng peptide extraction method |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106755247A (en) * | 2016-12-13 | 2017-05-31 | 国肽生物科技(北京)有限公司 | The extracting method of crocodile peptide |
| CN108402459A (en) * | 2018-03-06 | 2018-08-17 | 深圳施之健生命科技有限公司 | Snakehead Gallus domesticlus brisson book on Chinese herbal medicine composition and its preparation method and application |
| CN108402459B (en) * | 2018-03-06 | 2021-12-10 | 深圳施之健生命科技有限公司 | Snakehead and black-bone chicken herbal composition and preparation method and application thereof |
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