CN104605195B - A kind of feed additive for weaning piglet and its preparation method and application - Google Patents

A kind of feed additive for weaning piglet and its preparation method and application Download PDF

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CN104605195B
CN104605195B CN201510025538.2A CN201510025538A CN104605195B CN 104605195 B CN104605195 B CN 104605195B CN 201510025538 A CN201510025538 A CN 201510025538A CN 104605195 B CN104605195 B CN 104605195B
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feed
piglet
weaning
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feed additive
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CN104605195A (en
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王定发
周汉林
周璐丽
侯冠彧
周雄
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Tropical Crops Genetic Resources Institute CATAS
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Abstract

The present invention relates to domestic animal feed additive field, a kind of feed additive for weaning piglet and its preparation method and application is disclosed.Jia Betel extracts and carrier, feed addictive obtained are added in additive can be directly appended in weanling pig feed, can improve the growth performance of weanling pig, enhance anti-oxidant, the anti-inflammatory and immunocompetence of weanling pig, using convenient, significant effect.Feed additive for weaning piglet of the present invention is added, the work difficulty of weaning pig feeding is reduced, has saved feeding cost.

Description

A kind of feed additive for weaning piglet and its preparation method and application
Technical field
The present invention relates to pig domestic animal feed additive field, a kind of feed additive for weaning piglet and its preparation are particularly related to Methods and applications.
Background technology
Jia Betel(Piper sarmentosum), it is the plant of Piperaceae Piper, is distributed widely in India, Vietnam, India Nicaea, Philippine, Papua New Guinea, Malaysia and the Yunnan of China's Mainland, Guizhou, Fujian, Tibet, Guangdong, The ground such as Guangxi.Investigation shows , Jia Betel plants point in the Eco-Environment System in Hainan Province based on artificial natural rubber woods For cloth occupancy volume in occupation of the maximum group in shade plants type, distribution is relatively broad.
It is reported that each tissue such as Jia Betel roots, stem, leaf, fruit ear has effects that detoxification sore treatment, it is for oral administration effectively to treat abdomen The illnesss such as swollen, enteritis, diarrhea, rheumatalgia, external application can treat bleeding caused by wound, silt blueness and pernio etc..Recent study table Bright , Folium Piperis sarmentosis water-soluble extractive has anti-inflammatory activity, and the amides compound in extract has apparent anti-oxidant, anti- Tuberculous acts on.
Weaned piglet is a vital stage during feeding piglet.During wean, due in food gradually Feed is added, the incidence of the diseases such as grice diarrhoea greatly increases, and growth performance substantially reduces, and has seriously affected the growth of piglet Development.Therefore, improve weanling pig intestinal immunity early-weaned piglets are smoothly spent early weaning close it is most important.
Invention content
The present invention proposes a kind of feed additive for weaning piglet and its preparation method and application, has and improves weanling pig Immunity, the effect for improving piglet growth performance.
The technical proposal of the invention is realized in this way:
A kind of feed additive for weaning piglet, including following component:Jia Betel extracts and carrier.
Further, the Jia Betel extract formulations, include the component of following parts by weight:1-3 parts of Jia Betel extracts carry 1-3 parts of body.
Further, the carrier is silica or calcium carbonate.
Further, the Jia Betel extracts are made as dry in the shade Folium Piperis sarmentosi piece supercritical carbon dioxide extraction methods or Soxhlet Extraction method obtains Jia Betel extracts.
The preparation method of the feed additive for weaning piglet, includes the following steps:
Formula ratio Jia Betel extracts and carrier are taken, is uniformly mixed and is added to get weanling pig feed of the present invention Agent.
Application of the feed additive for weaning piglet on preparing the feed for improving Growth Performance of Weaning Piglets.
Application of the feed additive for weaning piglet on preparing the feed for improving weanling pig immunity.
Application of the feed additive for weaning piglet on preparing the feed for improving weanling pig anti-inflammatory power.
Application of the feed additive for weaning piglet on preparing the feed for improving oxidation resistance.
Further, the feed additive for weaning piglet dosage is that per kilogram feed adds 100-200 mg.
Beneficial effects of the present invention:
Feed additive for weaning piglet of the present invention contains asarone, cinnamic acid, meat in Jia Betel extracts therein The plurality of active ingredients such as cardamom ether, carypohyllene, phellandrene have the effects that antibacterial anti-inflammatory, anti-oxidant, promotion peristalsis of the digest tract. But since gained Jia Betel extracts are in oily, are added in feed and are difficult to be uniformly mixed, influence quality of the fodder, it is also unfavorable It searches for food in piglet.The carrier added in the present invention has absorption, diluted effect, can adsorb oily Jia Betel extracts, energy It is enough effectively to avoid generating caking after being added in feed, there is the problems such as mixing is uneven.
Feed additive for weaning piglet proposed by the present invention is added in weanling pig feed, the growth of weanling pig It can greatly increase, oxidation resistance, anti-inflammatory power and immunity are improved, and can effectively improve the digestive system of weanling pig Function, enhancing weanling pig reduce various diseases and help weanling pig and smoothly spend early stage to the resistivity of disease Weaning period reduces the work difficulty of weaning pig feeding, has saved feeding cost.
Specific implementation mode
Below in conjunction with the embodiment of the present invention, the technical solution in the present invention is clearly and completely described, it is clear that Described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.Based on the implementation in the present invention Example, the every other embodiment that those of ordinary skill in the art are obtained without creative efforts belong to The scope of protection of the invention.
Embodiment 1
A kind of feed additive for weaning piglet, including following component:Jia Betel extracts 10 g, 0 g of silica 1;It is described The Jia Betel extracts Folium Piperis sarmentosis piece that is made as drying in the shade obtains Jia Betel extracts with supercritical carbon dioxide extraction method;Specific extraction Condition is as follows:The granularity of Jia Betel cauline leaf powder is 20-40 mesh, and extracting pressure 15-40MPa, extraction temperature is 35-60 °C, dioxy Change carbon flow is 40-60L/h, and extraction time is 1.0-3.0 h.
The preparation method of the feed additive for weaning piglet, includes the following steps:
The oily Jia Betel extracts and carrier of formula ratio are taken, is uniformly mixed to get weanling pig feed of the present invention Additive.
Above-mentioned obtained feed additive for weaning piglet, can be directly appended in weanling pig feed, additive amount is every Kg diet adds 100 mg, and feed obtained, which has, improves pigling immunity, anti-inflammatory power, oxidation resistance, and can Improve the growth performance of weanling pig.
Embodiment 2
A kind of feed additive for weaning piglet, including following component:30 g of Jia Betel extracts, silica or calcium carbonate 10 G, the Jia Betel extracts are made as drying in the shade the oily Jia Betel objects that Folium Piperis sarmentosis piece is obtained with supercritical carbon dioxide extraction method;Tool Body extraction conditions are same as Example 1
The preparation method of the feed additive for weaning piglet, includes the following steps:
Formula ratio Jia Betel extracts and carrier are taken, is uniformly mixed and is added to get weanling pig feed of the present invention Agent.
Above-mentioned obtained feed additive for weaning piglet, can be directly appended in weanling pig feed, additive amount is every Kg diet adds 100 mg, and feed obtained, which has, to be improved pigling immunity, improves piglet growth performance, and can improve wean Anti-oxidant, the anti-inflammatory power of piglet.
Embodiment 3
A kind of feed additive for weaning piglet, including following component:10 g of Jia Betel extracts, silica 30 g, it is described Jia Betel extracts are made as drying in the shade the oily Jia Betel objects that Folium Piperis sarmentosis piece is obtained with soxhlet extraction method;Concrete operations condition is: The granularity of Jia Betel cauline leaf powder is 20-40 mesh, the ethyl alcohol that volumetric concentration is 70-80% is added, Soxhlet reflux 2-3 h, it is in oily to be made Jia Betel extracts.
The preparation method of the feed additive for weaning piglet, includes the following steps:
Formula ratio Jia Betel extracts and carrier are taken, is uniformly mixed and is added to get weanling pig feed of the present invention Agent.
Above-mentioned obtained feed additive for weaning piglet, can be directly appended in weanling pig feed, additive amount is every Kg diet adds 200 mg, and feed obtained, which has, to be improved pigling immunity, improves piglet growth performance, and can improve wean Anti-oxidant, the anti-inflammatory power of piglet.
Embodiment 4
A kind of feed additive for weaning piglet, including following component:20 g of Jia Betel extracts, silica 30 g, it is described The Jia Betel extracts Folium Piperis sarmentosis piece that is made as drying in the shade obtains Jia Betel objects with supercritical carbon dioxide extraction method;Specific extraction conditions It is same as Example 1.
The preparation method of the feed additive for weaning piglet, includes the following steps:
Formula ratio Jia Betel extracts and carrier are taken, is uniformly mixed and is added to get weanling pig feed of the present invention Agent.
Above-mentioned obtained feed additive for weaning piglet, can be directly appended in weanling pig feed, additive amount is every Kg diet adds 150 mg, and feed obtained, which has, to be improved pigling immunity, improves piglet growth performance, and can improve wean Anti-oxidant, the anti-inflammatory power of piglet.
Embodiment 5
A kind of feed additive for weaning piglet, including following component:30 g of Jia Betel extracts, calcium carbonate 20 g, it is described The Jia Betel extracts Folium Piperis sarmentosis piece that is made as drying in the shade obtains Jia Betel objects with soxhlet extraction.
The preparation method of the feed additive for weaning piglet, includes the following steps:
Formula ratio Jia Betel extracts and carrier are taken, is uniformly mixed and is added to get weanling pig feed of the present invention Agent.
Above-mentioned obtained feed additive for weaning piglet, can be directly appended in weanling pig feed, additive amount is every Kg diet adds 100 mg, and feed obtained, which has, to be improved pigling immunity, improves piglet growth performance, and can improve wean Anti-oxidant, the anti-inflammatory power of piglet.
Influence of the additive amount to Growth Performance of Weaning Piglets
Pair the weanling pig (21d wean) 80 that parity, Confinement Time are closer to is chosen, 4 processing groups are randomly divided into, i.e., According to group, 1 group is handled(T100), handle 2 groups(T200), handle 3 groups(T400), 4 repetitions are each handled, often repeatedly 5 pigs, often Repeat separated raising.Control group fed sucking pig material handles 1 group, 2 groups, 3 groups and adds 1 gained of embodiment in sucking pig material respectively Feed additive for weaning piglet 100,200 and 400 mg/kg.All piglets are allowed to be freely eaten and drink water, and feed 29 d.Sucking pig Any other medicated premix is no longer added in material.
Respectively with 30d early mornings on the day of on-test, the weighing piglet empty body weight as unit of each repetition;Daily with It is each to repeat to be that unit weighs feed relative, record piglet feed intake.Investigate influence of the different disposal to piglet growth performance.Its It the results are shown in Table 1.
Influence of the 1 feed addictive additive amount of table to Growth Performance of Weaning Piglets
Group Control group T100 T200 T400
First starting weight(kg) 6.39±0.01 6.43±0.03 6.47±0.06 6.43±0.05
Terminate weight(kg) 12.84±0.86ab 13.89±0.08a 12.24±0.31b 12.85±0.34ab
Average daily gain(g) 222.56±19.74b 257.39±3.65a 198.99±8.48b 221.52±10.03b
Average daily gain(g) 373.55±1.75c 421.95±4.63a 331.20±6.43d 387.64±5.63b
Feed-weight ratio 1.70±0.04 1.64±0.04 1.67±0.10 1.75±0.10
Note:Data mark difference lowercase letter indication difference of going together is notable(P<0.05), containing identical lowercase or without word Matrix shows that difference is not notable(P>0.05).
The result shows that the group piglet of 100 mg/kg of addition and 200 mg/kg feed additive for weaning piglet has higher put down Equal daily gain, and feed-weight ratio also decreases;And the group of 400 mg/kg feed additive for weaning piglet is added, although piglet Average daily gain is also improved, but feed-weight ratio is higher.Therefore consider feed cost, heretofore described weanling pig is raised The additive amount of feed additives is 100-200 mg/kg.
Influence of the feed additive for weaning piglet of gained of the invention to Growth Performance of Weaning Piglets
It chooses parity, the weanling pig (21d wean) 120 that Confinement Time is closer to, is randomly divided into 6 processing groups, i.e., Control group handles 1 group(T1), handle 2 groups(T2), handle 3 groups(T3), handle 4 groups(T4), handle 5 groups(T5), each handle 4 A repetition, often repeatedly 5 pigs, often repeatedly separated raising.Control group fed sucking pig material, 5 processing groups are distinguished in sucking pig material respectively Feed addictive made from 5 embodiments is added, and is added in weanling pig feed according to corresponding additive capacity.All sons Pig is allowed to be freely eaten and drink water, and feeds 29d.Any other medicated premix is no longer added in sucking pig material.
Piglet empty stomach body is weighed as unit of each repetition with the 30th d early morning off-tests on the day of on-test respectively Weight;Feed relative is weighed as unit of each repetition daily, records piglet feed intake.Different disposal is investigated to piglet growth performance Influence.The result is shown in tables 2.
Influence of the feed addictive of 2 present invention gained of table to Growth Performance of Weaning Piglets
Group First starting weight(kg) Terminate weight(kg) Average daily gain(g) Average daily gain(g) Feed-weight ratio
Control group 6.39±0.01 12.84±0.86 222.56±19.74bc 373.55±1.75c 1.70±0.04
T1 6.43±0.03 13.89±0.08 257.39±3.65a 421.95±4.63a 1.64±0.04
T2 6.41±0.07 13.09±0.25 230.27±6.58bc 390.65±4.10b 1.70±0.06
T3 6.47±0.10 13.09±0.18 228.39±6.79bc 386.33±6.07b 1.69±0.06
T4 6.45±0.06 13.45±0.20 241.46±5.36b 396.33±4.92b 1.64±0.04
T5 6.45±0.08 13.32±0.34 236.76±9.34bc 389.33±7.79b 1.64±0.03
Note:It is notable with column data mark difference lowercase letter indication difference(P<0.05), containing identical lowercase or without word Matrix shows that difference is not notable(P>0.05).Similarly hereinafter
The result shows that being compared with control group, the group weanling pig of the feed additive for weaning piglet obtained by embodiment is added There are higher average daily gain and daily ingestion amount, the wherein average daily gain of T1 processing group and feed intake increase is most notable, and compares Group significant difference.Meanwhile compared with the control group, the feed-weight ratio of processing group also decreases.Illustrate, the wean of present invention gained is young Pig feed additive can improve the growth performance of weanling pig, improve the digestion power of weanling pig, carry to a certain extent The utilization rate of high feed.
Influence of the feed additive for weaning piglet of gained of the invention to weanling pig oxidation resistance
It chooses parity, the weanling pig (21d wean) 120 that Confinement Time is closer to, is randomly divided into 6 processing(I.e. pair According to group, 1 group is handled(T1), handle 2 groups(T2), handle 3 groups(T3), handle 4 groups(T4), handle 5 groups(T5)), each processing 4 It repeats, often repeatedly 5 pigs, often repeatedly separated raising.Control group fed sucking pig material, 5 processing groups add in sucking pig material respectively respectively Add feed addictive made from 5 embodiments, and is added in weanling pig feed according to corresponding additive capacity.All piglets It is allowed to be freely eaten and drink water, feed 29d.Any other medicated premix is no longer added in sucking pig material.
In 14d, 30d early morning, often repeating to randomly select 2 pigs, jugular vein blood collection, 37 DEG C stand 2h, 4 DEG C of 4h, centrifuge, 3500rpm centrifuges 8 min, and serum, -20 DEG C of preservations are precipitated.Measure MDA, T-SOD, GSH-Px concentration in serum.Investigate the present invention Influence of the feed additive for weaning piglet of gained to weanling pig oxidation resistance.The result is shown in tables 3 and table 4.
The 14th d weanling pig Serum antioxidant indices of table 3
Group SOD(U/ml) GSH-Px(U/ml) MDA(nmol/ml)
Control group 108.33±2.92b 333.63±39.78 5.40±0.20a
T1 126.52±5.56a 389.93±31.23 3.02±0.80c
T2 110.42±3.03b 408.30±32.31 4.26±0.44b
T3 108.27±5.77b 384.00±22.20 4.44±0.15b
T4 115.35±4.65b 385.56±28.95 4.17±0.23bc
T5 118.87±5.34b 359.11±37.85 3.95±0.35bc
The 30th d weanling pig Serum antioxidant indices of table 4
Group SOD(U/ml) GSH-Px(U/ml) MDA(nmol/ml)
Control group 105.25±3.12b 522.11±35.79b 4.38±0.05a
T1 111.75±2.83a 637.89±42.11a 2.86±0.21c
T2 108.26±3.30ab 566.32±31.01ab 2.70±0.22c
T3 107.75±2.35ab 536.84±23.73b 2.73±0.27c
T4 109.75±2.19a 549.47±35.32ab 3.27±0.18b
T5 108.75±3.50ab 551.58±30.79ab 3.11±0.21bc
The result shows that being compared with control group, the group for adding the feed additive for weaning piglet obtained by the embodiment of the present invention is young There is SOD and GSH-Px concentration in Swine serum increases in various degree, and MDA concentration reduces.Illustrate to add feed addictive of the present invention The antioxygenic property of weanling pig is improved in various degree.
Influence of the feed additive for weaning piglet of gained of the invention to weanling pig inflammatory factor
It chooses parity, the weanling pig (21d wean) 120 that Confinement Time is closer to, is randomly divided into 4 processing(Control Group handles 1 group, handles 2 groups, handles 3 groups), 4 repetitions are each handled, often repeatedly 5 pigs, often repeatedly separated raising.Control group Sucking pig material is fed, 5 processing groups add feed addictive made from 5 embodiments respectively in sucking pig material respectively, and according to corresponding Additive capacity be added in weanling pig feed.All piglets are allowed to be freely eaten and drink water, and feed 29d.In sucking pig material not Any other medicated premix is added again.
In 14d, 30d early morning, often repeating to randomly select 2 pigs, jugular vein blood collection, 37 DEG C stand 2h, 4 DEG C of 4h, centrifuge, 3500rpm centrifuges 8 min, and serum, -20 DEG C of preservations are precipitated.Measure serum in proinflammatory cytokine IL-1 β, IL-6, TNF-α with And anti-inflammatory cytokines IL-4, IL-10, TGF-β concentration.The feed additive for weaning piglet for investigating present invention gained is young to wean The influence of pig anti-inflammatory power.The result is shown in tables 5 and table 6
The 14th d weanling pig serum cytokines contents (ng/L) of table 5
Group IL-1β IL-6 TNF-α IL-4 IL-10 TGF-β
Control group 2300±492a 51.43±5.15a 2260±30a 12.08±2.60c 58.51±8.36c 210.00±10.00b
T1 1033±152b 23.62±5.06c 1954±29c 19.25±0.66ab 64.71±2.94ab 236.67±10.41ab
T2 560±105c 29.76±5.27bc 2052±25b 21.25±3.31a 73.53±1.47a 248.33±20.21a
T3 783±208bc 45.71±4.71a 2122±14b 18.75±1.75ab 61.29±3.20b 225.00±15.50b
T4 750±125bc 35.17±3.76b 2068±17b 20.12±1.25a 60.20±4.27b 265±10.00a
T5 816±57b 30.57±2.65bc 1981±20c 23.78±2.21a 67.65±4.53ab 255.40±12.65a
The 30th d weanling pig serum cytokines contents (ng/L) of table 6
Group IL-1β IL-6 TNF-α IL-4 IL-10 TGF-β
Control group 2050±450a 23.33±5.36a 2145±44a 14.58±2.89b 58.65±6.30bc 238.33±5.77b
T1 1250±180b 14.29±3.78b 1939±11c 27.50±5.00a 71.76±3.54ab 295.37±15.65a
T2 766±104c 17.14±3.11ab 2029±10b 35.00±4.51a 75.51±3.83a 251.67±17.56b
T3 1100±180b 19.24±1.28ab 2023±16b 16.25±3.25b 63.35±1.94b 245.00±5.00b
T4 850±230bc 18.57±3.29ab 2050±14b 32.50±2.58a 65.34±3.24b 310.00±15.00a
T5 800±185bc 20.71±1.43a 1944±14c 30.00±4.50a 70.62±5.88ab 270.85±12.35a
The result shows that the feed additive for weaning piglet group obtained by the addition embodiment of the present invention is compared with control group, piglet Anti-inflammatory cytokines IL-4, IL-10 and TGF-β concentration in serum are improved, and reduces proinflammatory cytokine IL-1 β, IL-6 With TNF-α concentration.Illustrate that the feed additive for weaning piglet obtained by the embodiment of the present invention can enhance the anti-inflammatory energy of weanling pig Power.
Influence of the feed additive for weaning piglet of gained of the invention to weanling pig immunity
It chooses parity, the weanling pig (21d wean) 120 that Confinement Time is closer to, is randomly divided into 4 processing(Control Group handles 1 group, handles 2 groups, handles 3 groups), 4 repetitions are each handled, often repeatedly 5 pigs, often repeatedly separated raising.Control group Sucking pig material is fed, 5 processing groups add feed addictive made from 5 embodiments respectively in sucking pig material respectively, and according to corresponding Additive capacity be added in weanling pig feed.All piglets are allowed to be freely eaten and drink water, and feed 29d.
In 14d, 30d early morning, often repeating to randomly select 2 pigs, jugular vein blood collection, 37 DEG C stand 2h, 4 DEG C of 4h, centrifuge, 3500rpm centrifuges 8 min, and serum, -20 DEG C of preservations are precipitated.Measure IgA, IgG and IgM concentration in serum.Investigate present invention gained Influence of the feed additive for weaning piglet to weanling pig immunity.The result is shown in tables 7 and table 8.
The 14th d weanling pigs Immunoglobulins in Serum (g/L) of table 7
Group IgM IgG IgA
Control group 0.37±0.06c 2.30±0.14b 0.01±0.00b
T1 0.74±0.06a 2.63±0.16a 0.04±0.01a
T2 0.45±0.07bc 2.40±0.10ab 0.02±0.01ab
T3 0.71±0.09a 2.48±0.06ab 0.02±0.01ab
T4 0.50±0.02b 2.52±0.02ab 0.03±0.01ab
T5 0.51±0.03b 2.35±0.04b 0.03±0.01ab
The 30th d weanling pigs Immunoglobulins in Serum (g/L) of table 8
Group IgM IgG IgA
Control group 0.45±0.07b 2.03±0.14b 0.01±0.00b
T1 0.74±0.06a 2.84±0.07a 0.04±0.01a
T2 0.57±0.04ab 2.34±0.12b 0.03±0.01ab
T3 0.51±0.08ab 2.51±0.08ab 0.02±0.01ab
T4 0.52±0.03ab 2.54±0.09ab 0.04±0.01a
T5 0.55±0.04ab 2.40±0.06b 0.03±0.01ab
The result shows that adding group piglet and the control group phase of the feed additive for weaning piglet obtained by the embodiment of the present invention Than piglet Immunoglobulin in Serum content has different degrees of raising.
In summary test result adds the feed additive for weaning piglet of present invention gained, Growth Performance of Weaning Piglets It gets a promotion, weanling pig also improves to the utilization rate of feed, and digestive system function is improved, and is that piglet is smooth Spending the early weaning phase improves guarantee, and weanling pig digestive system is enable to adapt to forage feed as early as possible, reduces digestive system The generation of disease reduces the raising difficulty of weanling pig.In addition, the feed additive for weaning piglet of addition present invention gained, Oxidation resistance, anti-inflammatory power, the immunity of weanling pig have different degrees of raising, enhance weanling pig to disease Immunity reduces the generation of various diseases, has saved feeding cost.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all essences in the present invention With within principle, any modification, equivalent replacement, improvement and so on should all be included in the protection scope of the present invention god.

Claims (2)

1. a kind of application of feed additive for weaning piglet on preparing the feed for improving Growth Performance of Weaning Piglets, described is disconnected Milk additive for feed for piglets is made of the component of following parts by weight:1 part of Jia Betel extracts, 1 part of carrier, the Jia Betel extractions Object is that the Folium Piperis sarmentosis piece that dries in the shade extracts Jia Betel extracts with supercritical carbon dioxide extraction method, and the carrier is dioxy SiClx or calcium carbonate, the feed additive for weaning piglet dosage are that per kilogram feed adds 100mg.
2. a kind of application of feed additive for weaning piglet on preparing the feed for improving weanling pig immunity, the wean Additive for feed for piglets is made of the component of following parts by weight:1 part of Jia Betel extracts, 1 part of carrier, the Jia Betel extracts To dry in the shade, Folium Piperis sarmentosis piece extracts Jia Betel extracts with supercritical carbon dioxide extraction method, and the carrier is titanium dioxide Silicon or calcium carbonate, the feed additive for weaning piglet dosage are that per kilogram feed adds 100mg.
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