CN104593520A - 一种肺癌miRNA检测试剂盒及其miRNA的应用 - Google Patents
一种肺癌miRNA检测试剂盒及其miRNA的应用 Download PDFInfo
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- 239000002679 microRNA Substances 0.000 title claims abstract description 53
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- 108091062762 miR-21 stem-loop Proteins 0.000 claims abstract description 28
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
- C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
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- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/178—Oligonucleotides characterized by their use miRNA, siRNA or ncRNA
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Abstract
Description
组分 | 体积 | 使用浓度 |
内参miRNA-39液 | 10μl | 0.2μM |
miR-39正向引物 | 100μl | 2μM |
组分 | 体积 | 使用浓度 |
polyA polymerase | 50μl | 2.5U/μl |
RTase Mix | 50μl | 原商品浓度 |
5×PAP/RT buffer | 250μl | 原商品浓度 |
RNase and DNase free H2O | 1000μl | — |
组分 | 体积 | 使用浓度 |
2×SYBR Green Mix | 500μl | 原商品浓度 |
各F primer | 各100μl | 2μM |
Universal Adaptor PCR Primer | 100μl | 2μM |
H2O | 1000μl | — |
组分 | 加入量 |
polyA polymerase | 0.5μl |
RTase Mix | 0.5μl |
5×PAP/RT buffer | 2.5μl |
miRNA样本 | 20ng |
Rnase and DNase free H2O | 补水至总体积为12.5μl |
组分 | 加入量 |
2×SYBR Green Mix | 5μl |
F primer | 1μl |
Universal Adaptor PCR Primer | 1μl |
cDNA模板 | 1μl |
Rnase and DNase free H2O | 补水至总体积为10μl |
Claims (7)
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Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108070654A (zh) * | 2017-04-13 | 2018-05-25 | 朱伟 | 肺癌miRNA检测的内参基因的鉴定 |
CN108165546A (zh) * | 2017-03-23 | 2018-06-15 | 上海立迪生物技术股份有限公司 | 一种miRNA生物标志物、组合物及其用途 |
CN110199024A (zh) * | 2016-11-25 | 2019-09-03 | 法国国家科学研究中心 | 治疗nsclc患者的药剂和预测反应的方法 |
CN110564859A (zh) * | 2019-06-13 | 2019-12-13 | 中国农业科学院兰州兽医研究所 | 一种用于片形吸虫感染检测的标志物及试剂盒 |
CN113930503A (zh) * | 2017-08-14 | 2022-01-14 | 江苏为真生物医药技术股份有限公司 | miR-126和miR-152联合在制备诊断和预示肺癌的试剂或试剂盒中的应用 |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109251964B (zh) * | 2018-04-25 | 2021-09-14 | 深圳市乾康医药科技有限公司 | 循环microRNAs检测试剂盒和特异性检测循环microRNAs的方法及应用 |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2012089630A1 (en) * | 2010-12-30 | 2012-07-05 | Fondazione Istituto Firc Di Oncologia Molecolare (Ifom) | A METHOD TO IDENTIFY ASYMPTOMATIC HIGH-RISK INDIVIDUALS WITH EARLY STAGE LUNG CANCER BY MEANS OF DETECTING miRNAs IN BIOLOGIC FLUIDS |
CN102899390A (zh) * | 2011-07-26 | 2013-01-30 | 中国科学院上海生命科学研究院 | 小细胞肺癌标志物及其检测 |
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2015
- 2015-02-28 CN CN201510090767.2A patent/CN104593520B/zh active Active
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
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WO2012089630A1 (en) * | 2010-12-30 | 2012-07-05 | Fondazione Istituto Firc Di Oncologia Molecolare (Ifom) | A METHOD TO IDENTIFY ASYMPTOMATIC HIGH-RISK INDIVIDUALS WITH EARLY STAGE LUNG CANCER BY MEANS OF DETECTING miRNAs IN BIOLOGIC FLUIDS |
CN102899390A (zh) * | 2011-07-26 | 2013-01-30 | 中国科学院上海生命科学研究院 | 小细胞肺癌标志物及其检测 |
Non-Patent Citations (1)
Title |
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KELLER A ET AL.: "miRNAs in lung cancer-studying complex fingerprints in patient"s blood cells by microarray experiments", 《BMC CANCER》, vol. 9, 6 October 2009 (2009-10-06), pages 1 - 10 * |
Cited By (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN110199024A (zh) * | 2016-11-25 | 2019-09-03 | 法国国家科学研究中心 | 治疗nsclc患者的药剂和预测反应的方法 |
CN108165546A (zh) * | 2017-03-23 | 2018-06-15 | 上海立迪生物技术股份有限公司 | 一种miRNA生物标志物、组合物及其用途 |
CN108070654A (zh) * | 2017-04-13 | 2018-05-25 | 朱伟 | 肺癌miRNA检测的内参基因的鉴定 |
CN108070654B (zh) * | 2017-04-13 | 2019-03-01 | 朱伟 | 肺癌miRNA检测的内参基因的鉴定 |
CN113930503A (zh) * | 2017-08-14 | 2022-01-14 | 江苏为真生物医药技术股份有限公司 | miR-126和miR-152联合在制备诊断和预示肺癌的试剂或试剂盒中的应用 |
CN113930503B (zh) * | 2017-08-14 | 2024-01-23 | 江苏为真生物医药技术股份有限公司 | miR-126和miR-152联合在制备诊断和预示肺癌的试剂或试剂盒中的应用 |
CN110564859A (zh) * | 2019-06-13 | 2019-12-13 | 中国农业科学院兰州兽医研究所 | 一种用于片形吸虫感染检测的标志物及试剂盒 |
CN110564859B (zh) * | 2019-06-13 | 2022-06-24 | 中国农业科学院兰州兽医研究所 | 一种用于片形吸虫感染检测的标志物及试剂盒 |
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Denomination of invention: A lung cancer miRNA detection kit and its application of miRNA Effective date of registration: 20231201 Granted publication date: 20161005 Pledgee: Industrial Bank Co.,Ltd. Shanghai Changning sub branch Pledgor: BASETRA MEDICAL TECHNOLOGY CO.,LTD. Registration number: Y2023310000796 |