CN104558227A - Method for extracting ganoderan - Google Patents
Method for extracting ganoderan Download PDFInfo
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- CN104558227A CN104558227A CN201410778126.1A CN201410778126A CN104558227A CN 104558227 A CN104558227 A CN 104558227A CN 201410778126 A CN201410778126 A CN 201410778126A CN 104558227 A CN104558227 A CN 104558227A
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Abstract
The invention discloses a method for extracting ganoderan. The method comprises the following steps: (1) pretreatment; (2) crude extraction; (3) freezing extraction; (4) alcohol precipitation; (5) protein removal; (6) decolorization; (7) ultrafiltration; and (8) vacuum drying. The method provided by the invention is simple to operate and low in production cost, and the extracted ganoderan is stable in quality and good in activity, is safe, and can be widely used in the fields of health products, biological medicines and the like.
Description
Technical field
The present invention relates to Pharmaceutical Polysaccharides and extract field, specifically a kind of Extraction method of ganoderan.
Background technology
The fungus resource of China is very abundant, and fungi is the precious resources storehouse finding novel drugs.Wherein, the primary medicinal component ganoderan of glossy ganoderma has one and strengthens the body resistance to consolidate the constitution, the effect of strengthening by means of tonics, is widely used for treating hepatopathy, ephrosis, hypertension, hyperlipidemia, neurasthenia etc., and achieves good clinical efficacy.In the Application and Development of ganoderan, separation and Extraction is one of step of key, and it not only affects the purification rate of product, and is directly connected to economic benefit, is also worth closely related with the activity of product.Traditional extracting method not only extraction yield is low, and purity is inadequate, and medical active is also bad.
Summary of the invention
For solving the problems of the technologies described above, the invention provides a kind of simple to operate, production cost is low, the Extraction method of ganoderan that refining effect is good.
The present invention is by the following technical solutions:
An extracting method for ganoderan, comprises the following steps:
(1) pre-treatment: add 95% alcohol immersion 3 days in Ganoderma sporophore, elimination ethanol.
(2) slightly extract: in the Ganoderma sporophore that Ethanol Treatment is crossed, add prozyme, at 40 ~ 60 DEG C, process 2-4h, add the distilled water of 12-15 times amount, 100 degree of refluxing extraction 1-2h, repeat 2 times, centrifuging and taking supernatant liquor after united extraction liquid.
(3) freezing extraction: the supernatant liquor that step (2) obtains is placed 24h under 0 degree, forms flocculation sediment, with whizzer with the centrifugal 15-20min of 1000r/min, crosses and filters precipitation, obtain filtrate.
(4) alcohol precipitation: place after the filtrate that step (3) obtains under agitation being added 80% ethanol and separate out precipitation, cryodrying.
(5) removing protein: be dissolved in distilled water by dried precipitation, adds tan-liquor under stirring, boils solids removed by filtration impurity after 15min, obtains supernatant liquor.
(6) decolour: add activated carbon decolorizing in the supernatant liquor obtained after tannic acid process, heat filtering collects filtrate.
(7) ultrafiltration: the filtrate after decolouring is carried out ultrafiltration, molecular weight cut-off 30000, filtered solution concentrates.
(8) vacuum-drying: the ganoderan of purifying will be obtained after the concentrated solution vacuum-drying of step (7).
In step (2) described composite enzyme solution, prozyme is cellulase and polygalacturonase.
Prozyme add-on described in step (2) is 20 microlitres/g sporophore,
Described in step (5), the concentration of tan-liquor is 0.2%-0.3%
Beneficial effect of the present invention: in scheme provided by the invention, first with after Ethanol Treatment, to remove lipid material, makes Glycosylase lose activity simultaneously, prevents the degraded of polysaccharide; Adopt complex enzyme degradation outer layer fiber and pectin, be more conducive to the extraction of polysaccharide, the extraction yield of ganoderan and content can be made to obtain larger raising.
Embodiment
Below in conjunction with embodiment, the present invention is further elaborated, but the scope of protection of present invention is not limited to following embodiments.
Embodiment 1
An extracting method for ganoderan, comprises the following steps:
(1) pre-treatment: add 95% alcohol immersion 3 days in Ganoderma sporophore, elimination ethanol.
(2) slightly extract: the composite enzyme solution adding cellulase and polygalacturonase in the Ganoderma sporophore that Ethanol Treatment is crossed, add-on is 20 microlitres/g sporophore, then 2h is processed at 40 DEG C, add the distilled water of 12 times amount, 100 degree of refluxing extraction 1h, repeat 2 times, centrifuging and taking supernatant liquor after united extraction liquid.
(3) freezing extraction: the supernatant liquor that step (2) obtains is placed 24h under 0 degree, forms flocculation sediment, with whizzer with the centrifugal 15min of 1000r/min, crosses and filters precipitation, obtain filtrate.
(4) alcohol precipitation: place after the filtrate that step (3) obtains under agitation being added 80% ethanol and separate out precipitation, cryodrying.
(5) removing protein: be dissolved in distilled water by dried precipitation, adding mass concentration under stirring is 0.2% tan-liquor, boils solids removed by filtration impurity after 15min, obtains supernatant liquor.
(6) decolour: add activated carbon decolorizing in the supernatant liquor obtained after tannic acid process, heat filtering collects filtrate.
(7) ultrafiltration: the filtrate after decolouring is carried out ultrafiltration, molecular weight cut-off 30000, filtered solution concentrates.
(8) vacuum-drying: the ganoderan of purifying will be obtained after the concentrated solution vacuum-drying of step (7).
Embodiment 2
An extracting method for ganoderan, comprises the following steps:
(1) pre-treatment: add 95% alcohol immersion 3 days in Ganoderma sporophore, elimination ethanol.
(2) slightly extract: the composite enzyme solution adding cellulase and polygalacturonase in the Ganoderma sporophore that Ethanol Treatment is crossed, add-on is 20 microlitres/g sporophore, then 4h is processed at 60 DEG C, add the distilled water of 15 times amount, 100 degree of refluxing extraction 2h, repeat 2 times, centrifuging and taking supernatant liquor after united extraction liquid.
(3) freezing extraction: the supernatant liquor that step (2) obtains is placed 24h under 0 degree, forms flocculation sediment, with whizzer with the centrifugal 20min of 1000r/min, crosses and filters precipitation, obtain filtrate.
(4) alcohol precipitation: place after the filtrate that step (3) obtains under agitation being added 80% ethanol and separate out precipitation, cryodrying.
(5) removing protein: be dissolved in distilled water by dried precipitation, adding mass concentration under stirring is 0.3% tan-liquor, boils solids removed by filtration impurity after 15min, obtains supernatant liquor.
(6) decolour: add activated carbon decolorizing in the supernatant liquor obtained after tannic acid process, heat filtering collects filtrate.
(7) ultrafiltration: the filtrate after decolouring is carried out ultrafiltration, molecular weight cut-off 30000, filtered solution concentrates.
(8) vacuum-drying: the ganoderan of purifying will be obtained after the concentrated solution vacuum-drying of step (7).
Embodiment 3
An extracting method for ganoderan, comprises the following steps:
(1) pre-treatment: add 95% alcohol immersion 3 days in Ganoderma sporophore, elimination ethanol.
(2) slightly extract: the composite enzyme solution adding cellulase and polygalacturonase in the Ganoderma sporophore that Ethanol Treatment is crossed, add-on is 20 microlitres/g sporophore, then 3h is processed at 50 DEG C, add the distilled water of 14 times amount, 100 degree of refluxing extraction 1.5h, repeat 2 times, centrifuging and taking supernatant liquor after united extraction liquid.
(3) freezing extraction: the supernatant liquor that step (2) obtains is placed 24h under 0 degree, forms flocculation sediment, with whizzer with the centrifugal 18min of 1000r/min, crosses and filters precipitation, obtain filtrate.
(4) alcohol precipitation: place after the filtrate that step (3) obtains under agitation being added 80% ethanol and separate out precipitation, cryodrying.
(5) removing protein: be dissolved in distilled water by dried precipitation, adds the tan-liquor that mass concentration is 0.25% under stirring, boil solids removed by filtration impurity after 15min, obtain supernatant liquor.
(6) decolour: add activated carbon decolorizing in the supernatant liquor obtained after tannic acid process, heat filtering collects filtrate.
(7) ultrafiltration: the filtrate after decolouring is carried out ultrafiltration, molecular weight cut-off 30000, filtered solution concentrates.
(8) vacuum-drying: the ganoderan of purifying will be obtained after the concentrated solution vacuum-drying of step (7).
Above embodiment is only in order to illustrate that technical scheme of the present invention is not intended to limit, and to not departing from any amendment of spirit and scope of the invention or equivalent replacement, it all should be encompassed in the middle of right of the present invention.
Claims (4)
1. an extracting method for ganoderan, comprises the following steps:
(1) pre-treatment: add 95% alcohol immersion 3 days in Ganoderma sporophore, elimination ethanol;
(2) slightly extract: in the Ganoderma sporophore that Ethanol Treatment is crossed, add prozyme, at 40 ~ 60 DEG C, process 2-4h, add the distilled water of 12-15 times amount, 100 degree of refluxing extraction 1-2h, repeat 2 times, centrifuging and taking supernatant liquor after united extraction liquid;
(3) freezing extraction: the supernatant liquor that step (2) obtains is placed 24h under 0 degree, forms flocculation sediment, with whizzer with the centrifugal 15-20min of 1000r/min, crosses and filters precipitation, obtain filtrate;
(4) alcohol precipitation: place after the filtrate that step (3) obtains under agitation being added 80% ethanol and separate out precipitation, cryodrying;
(5) removing protein: be dissolved in distilled water by dried precipitation, adds tan-liquor under stirring, boils solids removed by filtration impurity after 15min, obtains supernatant liquor;
(6) decolour: add activated carbon decolorizing in the supernatant liquor obtained after tannic acid process, heat filtering collects filtrate;
(7) ultrafiltration: the filtrate after decolouring is carried out ultrafiltration, molecular weight cut-off 30000, filtered solution concentrates;
(8) vacuum-drying: the ganoderan of purifying will be obtained after the concentrated solution vacuum-drying of step (7).
2., according to a kind of Extraction method of ganoderan described in claim 1, it is characterized in that in step (2) described composite enzyme solution, prozyme is cellulase and polygalacturonase.
3. according to a kind of Extraction method of ganoderan described in claim 1, it is characterized in that, prozyme add-on described in step (2) is 20 microlitres/g sporophore.
4. according to a kind of Extraction method of ganoderan described in claim 1, it is characterized in that, described in step (5), the mass concentration of tan-liquor is 0.2-0.3%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410778126.1A CN104558227A (en) | 2014-12-17 | 2014-12-17 | Method for extracting ganoderan |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410778126.1A CN104558227A (en) | 2014-12-17 | 2014-12-17 | Method for extracting ganoderan |
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| CN104558227A true CN104558227A (en) | 2015-04-29 |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104982534A (en) * | 2015-05-08 | 2015-10-21 | 苏州葛家坞生物科技有限公司 | Ganoderma lucidum polysaccharide-containing infant formula milk powder |
| CN105017441A (en) * | 2015-08-31 | 2015-11-04 | 桂林茗兴生物科技有限公司 | Ganoderma lucidum polysaccharide extracting method |
| CN105233244A (en) * | 2015-11-06 | 2016-01-13 | 安徽生物肽产业研究院有限公司 | Compound silkworm chrysalis bioactive small peptide composition |
| CN109369823A (en) * | 2018-12-25 | 2019-02-22 | 黑龙江大学 | A kind of extraction separation method of Polysaccharide in Pleurotus eryngii |
-
2014
- 2014-12-17 CN CN201410778126.1A patent/CN104558227A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104982534A (en) * | 2015-05-08 | 2015-10-21 | 苏州葛家坞生物科技有限公司 | Ganoderma lucidum polysaccharide-containing infant formula milk powder |
| CN104982534B (en) * | 2015-05-08 | 2018-09-18 | 苏州葛家坞生物科技有限公司 | A kind of baby formula milk powder of addition ganoderma lucidum polysaccharide |
| CN105017441A (en) * | 2015-08-31 | 2015-11-04 | 桂林茗兴生物科技有限公司 | Ganoderma lucidum polysaccharide extracting method |
| CN105233244A (en) * | 2015-11-06 | 2016-01-13 | 安徽生物肽产业研究院有限公司 | Compound silkworm chrysalis bioactive small peptide composition |
| CN109369823A (en) * | 2018-12-25 | 2019-02-22 | 黑龙江大学 | A kind of extraction separation method of Polysaccharide in Pleurotus eryngii |
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Application publication date: 20150429 |
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