CN101817809A - Method for producing flavonoids by using pectin-extracted orange peel - Google Patents
Method for producing flavonoids by using pectin-extracted orange peel Download PDFInfo
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- CN101817809A CN101817809A CN200910256055A CN200910256055A CN101817809A CN 101817809 A CN101817809 A CN 101817809A CN 200910256055 A CN200910256055 A CN 200910256055A CN 200910256055 A CN200910256055 A CN 200910256055A CN 101817809 A CN101817809 A CN 101817809A
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Abstract
The invention discloses a method for extracting, separating and purifying flavonoids from orange peel. In the method, the method for extracting an organic solvent is adopted and extraction is performed under the most optimal process conditions of extraction temperature of 90 DEG C, extraction time of 5 hours, 3 extraction times, 95 percent of alcohol concentration and a ratio of liquid to material of 1:30. The separation and purification adopt macroporous absorbent resin distributed elution to finally obtain orange flavonoids having purity of 90 percent. The method has the advantages of obtaining relatively higher yield and purity, utilizing industrial wastes, reducing environmental pollution and fulfilling the aim of resource comprehensive utilization.
Description
Technical field:
The present invention relates to the extractive technique field of flavones, is a kind of method that flavones is produced in the peel of Citrus reticulata Blanco separation and purification behind the pectin of extracting of utilizing specifically.
Technical background:
Peel of Citrus reticulata Blanco has another name called dried orange peel, the TAO Hong-Jing meaning: " orange peel is good with old person of a specified duration " claims dried orange peel so practise.Traditional Chinese medicine thinks that dried orange peel has dispelling cold and eliminating phlegm, and cough-relieving is promoted the production of body fluid, and regulates the flow of vital energy to be good for the stomach, the logical effect of the temperature of loosing.Modern medicine study shows that the orange peel extract has anti-oxidant, and is anti-ageing, antitumor, and antianaphylaxis is antiviral, and anti-inflammatory prevents the cardiovascular disorder isoreactivity.What wherein mainly act in fact is flavonoid compound.Tangeritin is the natural green compound that extracts from peel of Citrus reticulata Blanco, and it is potential much to have immeasurable pharmacology to be worth to we mankind.According to research reports, the various physiological functions of tangeritin are as follows: (1) oxidation-resistance comprises that removing singly revolves oxygen, superoxide, hydroxyl radical free radical and other free radical etc.; (2) anticancer and antitumor action comprises that resistance is different, antiproliferative and anticancer invasion etc.; (3) prevention recycle system illness effect comprises strengthening capillary vessel toughness, suppresses platelet aggregation and regulates effects such as cardiovascular; (4) anti-inflammatory and anti-allergic effects; (5) anti-microbial effect comprises fungi and bacterium or the like.
It all is directly will press the dry peel of Citrus reticulata Blanco of orange juice as raw material that existing peel of Citrus reticulata Blanco extracts flavones, and contain a large amount of pectin in the peel of Citrus reticulata Blanco, as the pectin that is present in plant cell wall karyocyte internal layer, it is a kind of material that provides support for the inside plants cell, under existing extraction conditions, pectin can suppress the destruction of extracting solution to the inside plants cell, causes flavones extraction yield and purity all lower.
Summary of the invention:
The present invention has overcome the deficiency of above-mentioned prior art, and a kind of method that the peel of Citrus reticulata Blanco behind the pectin is produced flavones of extracting of utilizing is provided, yield and the lower problem of purity when mainly solving existing method and extracting flavones.
In order to achieve the above object, the present invention is achieved in that a kind of the utilization extract the method that the peel of Citrus reticulata Blanco behind the pectin is produced flavones, and its special character is that it comprises following processing step:
A raw material: the peel of Citrus reticulata Blanco that has extracted pectin in the industrial production;
B extracts: 95% the alcohol that adds 30 times of weight of peel of Citrus reticulata Blanco extracts, and 90 ℃ of temperature are extracted 5h, extract 3 times;
C filters: solid-liquid separation, remove waste residue, and keep extracting solution;
D purifying: extracting solution is adsorbed (AB-8) with macroporous resin;
The e dissolving: the alcohol with 80% will be adsorbed on the flavones dissolving on the resin column;
F concentrates: the spirituous solution of flavones is concentrated;
G alcohol reclaims: the alcohol in reclaiming extracting solution and concentrating;
H drying: the concentrated solution of flavones is carried out vacuum-drying, make the finished product.
Compared with the prior art a kind of method of utilizing the peel of Citrus reticulata Blanco that extracts behind the pectin to produce flavones of the present invention has outstanding substantive distinguishing features and marked improvement: adopt the extraction process of the flavones in the peel of Citrus reticulata Blanco behind the intact pectin of alcoholic extraction and the method for separation and purification, higher yield and purity have not only been obtained, make yield bring up to 3% by present 2%, purity brings up to 90% by present 70%, also industrial waste has been carried out utilization of waste material simultaneously, reduce environmental pollution, reached the purpose of comprehensive utilization of resources.
Embodiment:
In order to understand better and to implement, a kind of method that the peel of Citrus reticulata Blanco behind the pectin is produced flavones of extracting of utilizing of the present invention is described below in conjunction with embodiment.
Embodiment 1, the peel of Citrus reticulata Blanco of pectin will have been extracted in the industrial production, 95% the alcohol that adds 30 times of weight of peel of Citrus reticulata Blanco extracts, 90 ℃ of temperature, extract 5h, extract 3 times, filter then, solid-liquid separation, remove waste residue, keep extracting solution, extracting solution is adsorbed with macroporous resin, the macroporous resin model is AB-8, to be adsorbed on flavones dissolving on the resin column with 80% alcohol again, the spirituous solution with flavones concentrates then, reclaim extracting solution and concentrate in alcohol, concentrated solution with flavones carries out vacuum-drying at last, makes the finished product.
After testing, the flavones yield is 3%, and purity is 90%.
Claims (1)
1. one kind is utilized and extracts the method that the peel of Citrus reticulata Blanco behind the pectin is produced flavones, it is characterized in that it comprises following processing step:
A raw material: the peel of Citrus reticulata Blanco that has extracted pectin in the industrial production;
B extracts: 95% the alcohol that adds 30 times of weight of peel of Citrus reticulata Blanco extracts, and 90 ℃ of temperature are extracted 5h, extract 3 times;
C filters: solid-liquid separation, remove waste residue, and keep extracting solution;
The d purifying: extracting solution is adsorbed with macroporous resin, and the macroporous resin model is AB-8;
The e dissolving: the alcohol with 80% will be adsorbed on the flavones dissolving on the resin column;
F concentrates: the spirituous solution of flavones is concentrated;
G alcohol reclaims: the alcohol in reclaiming extracting solution and concentrating;
H drying: the concentrated solution of flavones is carried out vacuum-drying, make the finished product.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910256055A CN101817809A (en) | 2009-12-18 | 2009-12-18 | Method for producing flavonoids by using pectin-extracted orange peel |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910256055A CN101817809A (en) | 2009-12-18 | 2009-12-18 | Method for producing flavonoids by using pectin-extracted orange peel |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101817809A true CN101817809A (en) | 2010-09-01 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
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| CN200910256055A Pending CN101817809A (en) | 2009-12-18 | 2009-12-18 | Method for producing flavonoids by using pectin-extracted orange peel |
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| CN (1) | CN101817809A (en) |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102344429A (en) * | 2011-07-22 | 2012-02-08 | 浙江省农业科学院 | Method for extracting and purifying tangerine polymethoxyflavone |
| CN103263514A (en) * | 2013-05-23 | 2013-08-28 | 浙江省柑桔研究所 | Method for extracting flavones, low-molecule pectin and cellulose from orange peels in combined way |
| CN103304363A (en) * | 2013-06-18 | 2013-09-18 | 浙江省柑桔研究所 | Method for combined extraction of D-limonene, flavonoid and pectin from orange peel |
| CN103583936A (en) * | 2013-10-23 | 2014-02-19 | 浙江蓝美农业有限公司 | Blueberry jam added with citrus peel and preparation method thereof |
| CN104926767A (en) * | 2014-03-18 | 2015-09-23 | 五邑大学 | Method of purification preparation of citrus flavones by adopting macroporous adsorption resin |
| CN105663174A (en) * | 2016-01-05 | 2016-06-15 | 安徽宇宁生物科技有限公司 | Production technology for recovery of bioflavonoid from pectin extracted ethanol waste liquid |
| CN108129584A (en) * | 2017-12-27 | 2018-06-08 | 中国农业科学院农产品加工研究所 | It is a kind of to realize pectin and flavones while the method for extraction in citrus peel residue |
| CN111227247A (en) * | 2020-03-10 | 2020-06-05 | 中国农业科学院农产品加工研究所 | Citrus product with functions of improving intestinal flora structure and relieving atherosclerosis as well as preparation method and application of citrus product |
-
2009
- 2009-12-18 CN CN200910256055A patent/CN101817809A/en active Pending
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102344429A (en) * | 2011-07-22 | 2012-02-08 | 浙江省农业科学院 | Method for extracting and purifying tangerine polymethoxyflavone |
| CN102344429B (en) * | 2011-07-22 | 2013-11-20 | 浙江省农业科学院 | Method for extracting and purifying tangerine polymethoxyflavone |
| CN103263514A (en) * | 2013-05-23 | 2013-08-28 | 浙江省柑桔研究所 | Method for extracting flavones, low-molecule pectin and cellulose from orange peels in combined way |
| CN103304363A (en) * | 2013-06-18 | 2013-09-18 | 浙江省柑桔研究所 | Method for combined extraction of D-limonene, flavonoid and pectin from orange peel |
| CN103583936A (en) * | 2013-10-23 | 2014-02-19 | 浙江蓝美农业有限公司 | Blueberry jam added with citrus peel and preparation method thereof |
| CN103583936B (en) * | 2013-10-23 | 2014-12-03 | 浙江蓝美农业有限公司 | Blueberry jam added with citrus peel and preparation method thereof |
| CN104926767A (en) * | 2014-03-18 | 2015-09-23 | 五邑大学 | Method of purification preparation of citrus flavones by adopting macroporous adsorption resin |
| CN105663174A (en) * | 2016-01-05 | 2016-06-15 | 安徽宇宁生物科技有限公司 | Production technology for recovery of bioflavonoid from pectin extracted ethanol waste liquid |
| CN108129584A (en) * | 2017-12-27 | 2018-06-08 | 中国农业科学院农产品加工研究所 | It is a kind of to realize pectin and flavones while the method for extraction in citrus peel residue |
| CN111227247A (en) * | 2020-03-10 | 2020-06-05 | 中国农业科学院农产品加工研究所 | Citrus product with functions of improving intestinal flora structure and relieving atherosclerosis as well as preparation method and application of citrus product |
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Owner name: YANTAI ANDRE PECTIC CO., LTD. Free format text: FORMER OWNER: ANDELI PECTIN CO., LTD., YANTAI Effective date: 20110603 |
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Effective date of registration: 20110603 Address after: 264100 No. 889 Xincheng street, Muping District, Shandong, Yantai Applicant after: Yantai Andre Pectin Co., Ltd. Address before: 264100 No. 889 Xincheng street, Muping District, Shandong, Yantai Applicant before: Andeli Pectin Co., Ltd., Yantai |
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Open date: 20100901 |