CN104542966B - Acidified milk comprising Kefir grains lactobacillus of whole intestines effect and preparation method thereof - Google Patents
Acidified milk comprising Kefir grains lactobacillus of whole intestines effect and preparation method thereof Download PDFInfo
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a kind of acidified milk comprising Kefir grains lactobacillus of whole intestines effect and preparation method thereof, its raw material, which includes Kefir grains lactobacillus KL22 and streptococcus thermophilus KV30, its preparation method, to be included（1）The processing of raw material milk,（2）Fermentation,（3）The steps such as the preparation of acidified milk.Kefir grains lactobacillus KL22 and streptococcus thermophilus KV30 are with the addition of, the viscosity of acidified milk can be improved, itself and other bacterial strains are compounded, the function of improving enteron aisle well can be played.Obtained acidified milk has the features such as unique flavor, exocellular polysaccharide content is high, and the acidified milk can be good at promoting the growth of beneficial bacterium in enteron aisle, suppress the growth of harmful bacteria.The present invention is applied to the preparation of acidified milk.
Description
Technical field
The invention belongs to dairy produce field, is related to a kind of acidified milk, and in particular to a kind of whole intestines effect includes Kefir grains
Acidified milk of lactobacillus and preparation method thereof.
Background technology
Kefir grains are a kind of containing alcohol prepared by by the use of Kefir granule as leavening, acid and few using milk, goat milk as raw material
Measure the acidified milk of carbon dioxide, also known as posset.Kefir fermented milk has many prebiotic functions, can such as suppress on blood pressure
Rise, prevent heart disease, reduce serum cholesterol levels, prevention enterogastric diseases, antitumor, raising immunity of organisms etc..Make
For a kind of traditional dairy products, Kefir fermented milk has considerably long history, but it there is also some problems, first, be permitted so far
The countries and regions of more production Kefir fermented milks can not still break away from Kefir granule, be fermented as production Yoghourt using pure culture
Agent carries out industrialized production；Secondly, bacterium is mutually complicated in Kefir granule, and fermentation process is difficult to control, in the product especially produced
Saccharomycete be present, cause product storing and the problems such as swollen bag easily occurring in transportation, it is difficult to ensure the steady of product quality
It is fixed；Again, acetic acid bacteria and saccharomycete are contained in Kefir granule, so as to cause Kefir grains that there is special penetrating odor, it is difficult to quilt
China consumer receives.Therefore, the lactic acid bacteria of prebiotic function is screened from Kefir granule and is applied to acidified milk
Production, has very important realistic meaning.
The prior art that screening lactobacillus carries out acidified milk preparation from Kefir granule has Chinese invention patent
CN102056486A, disclose a kind of " novel fermented milk product and its application ", using in Kefir grains lactobacillus and related yeasts bacterium extremely
A kind of few fermentation is prepared, and the acidified milk can suppress the growth for the intestinal bacterium being had undesirable effect to enteral environment.But
It is that for the leavening used in the patent for Kefir grains lactobacillus and its saccharomycete, the probiotic properties of bacterial strain first are horizontal in strain
On, although being Kefir grains lactobacillus, very big otherness functionally be present in bacterial strain；Secondly as saccharomycetes to make fermentation
Distinctive yeasty notes, alcohol and CO can be produced2Specific stimulation flavor after being combined, this allows China consumer to the acidified milk
Acceptance level reduce.
Chinese invention patent CN102524387A is disclosed " a kind of Kefir fermented dairy product and its preparation technology ", is used
Strain be the agent of Kefir grains basal fermentation and saccharomycete particle（For Danisco and section's Hansen）, both are external import bacterium
Kind, using Kefir grains lactobacillus and saccharomycete as strain, due to saccharomycetes to make fermentation can produce distinctive yeasty notes, alcohol and
CO2, be not suitable for all groups, therefore, the technology disclosed in the patent document, which is applied, has limitation.
Therefore, the preparation that a kind of bacterial strain isolated from Kefir granule carries out acidified milk is studied, is significant
's.
The content of the invention
The technical problem to be solved in the present invention, be to provide a kind of whole intestines effect the acidified milk comprising Kefir grains lactobacillus and
Its preparation method, with the addition of Kefir grains lactobacillus KL22 and streptococcus thermophilus KV30, the viscosity of acidified milk can be improved, by its with
Other bacterial strains compound, and can play the function of improving enteron aisle well.Obtained acidified milk has unique flavor, exocellular polysaccharide
The features such as content is high, it can be good at promoting the growth of beneficial bacterium in enteron aisle, suppress the growth of harmful bacteria.
In order to solve the above technical problems, the technical solution used in the present invention is：
A kind of acidified milk for include Kefir grains lactobacillus of whole intestines effect, its raw material include Kefir grains lactobacillus KL22 with
Streptococcus thermophilus KV30.
A kind of as the present invention limits, described Kefir grains lactobacillus KL22（Lactobacilus kefiri）In
On September 16th, 2014, is preserved in China Committee for Culture Collection of Microorganisms's General Microbiological Culture collection, and preservation is compiled
Number：CGMCC No.9669, depositary institution address are：Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3.
Another as the present invention limits, described streptococcus thermophilus KV30（Streptococcus thermophilus）In September in 2014 16 days, China Committee for Culture Collection of Microorganisms common micro-organisms bacterium is preserved in
Kind collection, deposit number：CGMCC No.9670, depositary institution address are：Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3
Number.
Kefir grains lactobacillus KL22 and streptococcus thermophilus KV30 is isolated from the assorted Kefir granule taken back in Polish Cali, is led to
Bio-chemical characteristics and 16S rDNA homology analysis are crossed, its nucleotide sequence is compared on NCBI websites using BLAST,
KL22 bacterial strains 16S rDNA sequences have 99% similarity with Lactobacilus kefiri strain NM180-3, therefore
It is accredited as Kefir grains lactobacillus.KV30 bacterial strains 16S rDNA sequences have with Streptococcus thermophilus
100% similarity, therefore it is accredited as streptococcus thermophilus.
As the third restriction of the present invention, it includes the raw material of following parts by weight：
Fresh milk or reconstituted milk 400-500 parts,
Stabilizer 2-4 parts, white granulated sugar 35-39 parts,
Kefir grains lactobacillus KL22 1 × 105-3×107Cfu/ml,
Streptococcus thermophilus KV30 1 × 105-3×107Cfu/ml,
Wherein, reconstituted milk is that skimmed milk power and distilled water are 1 by weight:7-13 reduction is made.
As the further restriction of above-mentioned restriction, described stabilizer is in gelatin, pectin, xanthans or converted starch
A kind of, two or more combination.
As the further restriction of above-mentioned restriction, described Kefir grains lactobacillus KL22 and streptococcus thermophilus KV30 addition
Weight ratio is 0.5-3：1.
As the further restriction of above-mentioned restriction, the raw material of described acidified milk also includes lactobacillus bulgaricus, thermophilic
One or more mixing in streptococcus, lactobacillus acidophilus, Bifidobacterium, Lactobacillus casei, Lactobacillus rhamnosus, total inoculum concentration
For the 1-5% of total acidified milk.
Present invention also offers the preparation method of above-mentioned acidified milk, and it is carried out according to following steps order：
A, it is uniform with stabilizer, white granulated sugar allotment by fresh milk or reconstituted milk, form material x；
B, deployed material x being subjected to pre- thermally homogenising, homogenizing temperature is 55-65 DEG C, homogenization pressure 15-20MPa, in
300s is sterilized at 93~97 DEG C, is cooled to 38~43 DEG C, forms material y；
（2）Fermentation
By material y inoculation Kefir grains lactobacillus KL22, streptococcus thermophilus KV30 and the other strains after cooling, sealing insulation
Fermentation, fermentation temperature are 30-42 DEG C, are 4.3-4.5 to pH scopes, form acidified milk p；
（3）The preparation of acidified milk
A, it is acidified milk p is stored refrigerated in 4 DEG C；
B, sterile filling, acidified milk is made.
A kind of as the present invention limits, and final obtained acidified milk, lactic acid bacteria sum is 1 × 107—8×108cfu/
Ml, viscosity scope are 150-300mPas.
As a result of above-mentioned technical scheme, compared with prior art, acquired technological progress is the present invention：
The present invention with the addition of Kefir grains lactobacillus KL22 and streptococcus thermophilus KV30, due to KL22 have well it is acidproof,
Bile tolerance and the effect for suppressing pathogen, KV30 have the glutinous characteristic of production well, and therefore, the collocation of two bacterial strains has regulation people very well
The beneficial function of body enteron aisle, the constraint of Kefir granule is broken away from, solved the shortcomings that directly preparing acidified milk using Kefir granule；
The viscosity of acidified milk can be improved by carrying out fermentation as strain using KL22 and KV30, itself and other bacterial strains be compounded, Ke Yiqi
To the good function of improving enteron aisle.Obtained acidified milk has the features such as unique flavor, exocellular polysaccharide content is high, the fermentation
Breast can be good at promoting the growth of beneficial bacterium in enteron aisle, suppress the growth of harmful bacteria.Simultaneously as in this special acidified milk not
Saccharomycete is added, therefore, can be very good to improve the stability of product, prevent the swollen bag during transport and storage.
The present invention is applied to the preparation of acidified milk.
The present invention is described in further detail below in conjunction with specific embodiment.
Embodiment
Acidified milk comprising Kefir grains lactobacillus of 1 whole intestines of embodiment effect and preparation method thereof
The acidified milk for including Kefir grains lactobacillus of whole intestines effect, its raw material include the raw material of following parts by weight：
Fresh milk 400g, distilled water 30g, gelatin 3g, white granulated sugar 35g,
Kefir grains lactobacillus KL22 2 × 105Cfu/ml, streptococcus thermophilus KV30 105cfu/ml。
The raw material of acidified milk also includes lactobacillus bulgaricus and Bifidobacterium, and total inoculum concentration is the 1% of total acidified milk.
Wherein, Kefir grains lactobacillus KL22 has been preserved in China Committee for Culture Collection of Microorganisms common micro-organisms bacterium
Kind collection, deposit number：CGMCC No.9669.
Streptococcus thermophilus KV30 has been preserved in China Committee for Culture Collection of Microorganisms's General Microbiological Culture preservation
Center, deposit number：CGMCC9670.
The preparation method of the acidified milk comprising Kefir grains lactobacillus of above-mentioned whole intestines effect, it enters according to following steps order
OK：
（1）The processing of raw material milk：
A, common raw material milk and stabilizer, white granulated sugar are allocated into uniform, formation material x；
B, deployed material x is subjected to pre- thermally homogenising, homogenizing temperature is 60 DEG C, homogenization pressure 15MPa, and sterilization is cold
But, sterilization temperature be 95 DEG C, sterilizing time 300s, be cooled to 40 DEG C, form material y；
（2）Fermentation
Material y after cooling is inoculated with Kefir grains lactobacillus KL22 and streptococcus thermophilus KV30, seals heat-preservation fermentation, fermentation
Temperature is 35 DEG C, is 4.5 to pH, forms acidified milk p；
（3）The preparation of acidified milk
A, it is acidified milk p is stored refrigerated in 4 DEG C；
B, sterile filling, the acidified milk for including Kefir grains lactobacillus of whole intestines effect is made.
The sum containing lactic acid bacteria is 8 × 10 in final obtained acidified milk7Cfu/ml, viscosity 200mPas.
By experiment, the raw material fresh milk in the present embodiment can be replaced with reconstituted milk, and the dosage of reconstituted milk is 400g, other
Raw material dosage and preparation method it is same as described above, reconstituted milk is whole milk powder, skimmed milk power or Evamilk albumen
It is 1 by weight with water：7 reduction are made, and in made acidified milk, lactic acid bacteria sum is 6 × 107Cfu/ml, viscosity are
230mPa·s。
Acidified milk comprising Kefir grains lactobacillus of the whole intestines effects of embodiment 2-9 and preparation method thereof
Embodiment 2-9 is respectively a kind of acidified milk comprising Kefir grains lactobacillus of whole intestines effect and preparation method thereof, with
The formula of embodiment 1 is similar with preparation method, the difference is that only：Institute in the component and dosage and preparation method that are wherein formulated
The technical parameter being related to is different, shown in table specific as follows：
Comparative example 1
The acidified milk that embodiment 1 is provided（1#）, Kefir granule acidified milk（2#）, lactobacillus bulgaricus and thermophilic chain
Coccus acidified milk（3#）Carry out suppressing pathogenic bacteria experiment.Respectively by 108Escherichia coli, shigella dysenteriae, golden yellow grape
Coccus is coated on MRS flat boards, is punched using card punch, respectively by the supernatant adding hole of above-mentioned three kinds of acidified milks
（Each sample do three it is parallel）, 37 DEG C of culture 24h, determine antibacterial circle diameter.Result of the test is as shown in the table：
The various acidified milk antibacterial circle diameters of table 1
As can be seen from the above table, compared with 2# samples, 1# samples, which have, is suppressing Escherichia coli, Staphylococcus aureus
Bacterium, shigella dysenteriae have more obvious effect, illustrate what is prepared using acidified milk prepared by KL22 with using Kefir granule
Difference is not present in acidified milk in terms of harmful bacteria is suppressed；And 3# has certain otherness compared with 1#, 2#, illustrate and common hair
Kefir milk is compared, and there is Kefir fermented milk the obvious harmful bacteria that suppresses to act on.
Comparative example 2
The acidified milk that embodiment 1 is provided（1#）, lactobacillus bulgaricus and streptococcus thermophilus fermentation breast（3#）Carry out
Zoopery, verify its experiment of improvement to gut flora.
30 mouse are chosen altogether, are randomly divided into 3 groups, 3 groups of given lows are 8g/kgBW；Once a day, continuous 14 days.
Microflora in the forward and backward inspection mouse intestinal of gavage, including：Bifidobacterium, lactobacillus, enterobacteria, enterococcus, perfringens shuttle
Bacterium.
The experimental results are shown inthe following table：
Influence of the 2 special acidified milk of table to the common Microflora of mouse intestinal
Note：## represents that same group of gavage is front and rear and pole significant difference, p itself be present<0.01；
# represents that same group of gavage is front and rear and significant difference, p itself be present<0.05；
* represents that the test group after gavage has pole significant difference, p compared with control group<0.01；
* represent that the test group after gavage has significant difference, p compared with control group<0.05.
As can be seen from the above table, 1# groups after gavage compared with control group, the increasing of bifidobacteria, lactobacillus quantity
Add and be respectively provided with statistical significance（Bifidobacterium is respectively p<0.05 and p<0.01, lactobacillus is respectively p<0.05 and p<0.01）；
Experiment it is front and rear itself relatively in, 1# groups bifidobacteria, which increases, has statistical significance（p<0.05）, 1# group enterobacteria quantity
Reduction there is statistical significance（Respectively p<0.05 and p<0.01）.3# groups before and after gavage itself and compared with control group,
Difference that there are no significant, illustrate Kefir fermented milk compared with common fermentation breast, have in terms of gut flora is improved obvious
Function.
The above described is only a preferred embodiment of the present invention, being not the limitation for making other forms to the present invention, appoint
What those skilled in the art is changed or is modified as equivalent variations possibly also with above-mentioned technology contents as enlightenment
Equivalent embodiment.But it is every without departing from technical solution of the present invention content, the technical spirit according to the present invention is to above example
Made simple modification, equivalent variations and remodeling, still fall within the protection domain of the claims in the present invention.
Claims (7)
- A kind of 1. acidified milk for including Kefir grains lactobacillus of whole intestines effect, it is characterised in that：Its raw material includes Kefir grains breast Bacillus KL22 and streptococcus thermophilus KV30；It also includes the raw material of following parts by weight：Fresh milk or reconstituted milk 400-500 parts,Stabilizer 2-4 parts, white granulated sugar 35-39 parts,Kefir grains lactobacillus KL22 1 × 105 -3×107Cfu/ml,Streptococcus thermophilus KV30 1 × 105 -3×107 Cfu/ml,Wherein, reconstituted milk is that skimmed milk power and distilled water are 1 by weight:7-13 reduction is made；Wherein, it is commonly micro- to be preserved in China Committee for Culture Collection of Microorganisms by described Kefir grains lactobacillus KL22 Biological inoculum collection, deposit number：CGMCC No.9669；Described streptococcus thermophilus KV30 has been preserved in China Committee for Culture Collection of Microorganisms's General Microbiological Culture Collection, deposit number：CGMCC No.9670.
- 2. the acidified milk for including Kefir grains lactobacillus of the whole intestines effect according to claim 1, it is characterised in that：Institute The stabilizer stated is one kind in gelatin, pectin, xanthans or converted starch, two or more combination.
- 3. the acidified milk for including Kefir grains lactobacillus of the whole intestines effect according to claim 1, it is characterised in that：Institute The Kefir grains lactobacillus KL22 and streptococcus thermophilus KV30 addition weight ratio stated is 0.5-3：1.
- 4. the acidified milk for including Kefir grains lactobacillus of the whole intestines effect according to claim 1, it is characterised in that：Institute The raw material for the acidified milk stated also includes lactobacillus bulgaricus, streptococcus thermophilus, lactobacillus acidophilus, Bifidobacterium, cheese breast bar One kind, two or more mixing in bacterium, Lactobacillus rhamnosus, total inoculum concentration are the 1-5% of total acidified milk.
- A kind of 5. system of the acidified milk comprising Kefir grains lactobacillus of whole intestines effect as any one of claim 1-4 Preparation Method, it is characterised in that it is carried out according to following steps order：（1）The processing of raw material milkA, it is uniform with stabilizer, white granulated sugar allotment by fresh milk or reconstituted milk, form material x；B, deployed material x being subjected to pre- thermally homogenising, homogenizing temperature is 55-65 DEG C, homogenization pressure 15-20MPa, in 300s is sterilized at 93~97 DEG C, is cooled to 38~43 DEG C, forms material y；（2）FermentationMaterial y inoculation Kefir grains lactobacillus KL22, streptococcus thermophilus KV30 after cooling and other strains, sealing are protected Temperature fermentation, fermentation temperature are 30-42 DEG C, are 4.3-4.5 to pH scopes, form acidified milk p；（3）The preparation of acidified milkA, it is acidified milk p is stored refrigerated in 4 DEG C；B, sterile filling, acidified milk is made.
- 6. the preparation method of the acidified milk comprising Kefir grains lactobacillus of whole intestines effect according to claim 5, its feature It is：Step（2）Described in other strains, including lactobacillus bulgaricus, streptococcus thermophilus, lactobacillus acidophilus, bifid One kind, two or more mixing in bacillus, Lactobacillus casei, Lactobacillus rhamnosus, total inoculum concentration are total acidified milk 1-5%.
- 7. the preparation method of the acidified milk comprising Kefir grains lactobacillus of the whole intestines effect according to claim 5, it is special Sign is：In final obtained acidified milk, lactic acid bacteria sum is 1 × 107 — 8×108Cfu/ml, viscosity 150- 300mPa·s。
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| CN106819107A (en) * | 2016-12-29 | 2017-06-13 | 石家庄君乐宝乳业有限公司 | Leben with the effect of whole intestines and preparation method thereof |
| CN106615116A (en) * | 2016-12-29 | 2017-05-10 | 石家庄君乐宝乳业有限公司 | Kefir yoghourt with probiotic function and preparation method thereof |
| CN107927168B (en) * | 2017-12-17 | 2021-03-26 | 石家庄君乐宝乳业有限公司 | Fermented milk containing lactobacillus kefir and preparation method thereof |
| CN108902310A (en) * | 2018-07-18 | 2018-11-30 | 吉林工商学院 | A kind of preparation method of Kefir grains fermentation purple perilla flavor yogurt |
| CN110742124A (en) * | 2019-12-02 | 2020-02-04 | 扬州大学 | Method for improving comprehensive bacteriostatic ability of milk wine fermentation liquor |
| CN112535214A (en) * | 2020-12-03 | 2021-03-23 | 石家庄君乐宝乳业有限公司 | Probiotic milk tablet with intestine regulating effect and preparation method thereof |
| CN114606157B (en) * | 2022-03-01 | 2023-07-14 | 湖北省微工生物技术研究有限公司 | Bifidobacterium microbial preparation and application thereof in kefir preparation |
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| BR102012032927A2 (en) * | 2012-12-21 | 2014-09-02 | Biologicus Indústria E Comércio De Produtos Naturais S/A | PROCESS FOR PRODUCTION OF GLYCERINATED EXTRACTS OF PROBIOTIC BIOACTIVES ADDED FROM AROMATIC ESSENTIAL OILS AND ANTIMICROBIAN CALLS PROBIIOTIC BIOACTIVE EXTRACTS (EBP) FOR USE IN COSMETIC PRODUCTS, BIOACTIC PRODUCTS, AND ALTERNATIVE PRODUCTS HUMAN AND ANIMAL BODY |
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| JP4519202B2 (en) * | 2008-06-10 | 2010-08-04 | 株式会社フィス | New fermented milk and its use |
| CN101720967A (en) * | 2008-11-03 | 2010-06-09 | 丹尼斯克（中国）有限公司 | Fruit and vegetable juice beverage fermented by Kefir fermenting agent and preparation method thereof |
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| CN102747000A (en) * | 2011-04-22 | 2012-10-24 | 天津科技大学 | Lactobacillus kefiranofaciens and its fermented milk preparation method |
| BR102012032927A2 (en) * | 2012-12-21 | 2014-09-02 | Biologicus Indústria E Comércio De Produtos Naturais S/A | PROCESS FOR PRODUCTION OF GLYCERINATED EXTRACTS OF PROBIOTIC BIOACTIVES ADDED FROM AROMATIC ESSENTIAL OILS AND ANTIMICROBIAN CALLS PROBIIOTIC BIOACTIVE EXTRACTS (EBP) FOR USE IN COSMETIC PRODUCTS, BIOACTIC PRODUCTS, AND ALTERNATIVE PRODUCTS HUMAN AND ANIMAL BODY |
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