CN104522089A - Green pollution-free plant compound preparation for preventing and treating virus, fungal and bacterial diseases of crops and preparation method and application thereof - Google Patents
Green pollution-free plant compound preparation for preventing and treating virus, fungal and bacterial diseases of crops and preparation method and application thereof Download PDFInfo
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Abstract
The invention relates to a green pollution-free plant compound preparation for preventing and treating virus, fungal and bacterial diseases of crops. The green pollution-free plant compound preparation for preventing and treating virus, fungal and bacterial diseases of the crops is prepared through, by weight, 100-300 parts of momordica grosvenori, 50-200 parts of polygonatum sibiricum, 50-200 parts of coptis chinensis, 500-1500 parts of asarum sieboldii, 1000-3000 parts of lonicera japonica, 1000-3000 parts of rosin, 500-1500 parts of folium ginkgo, 500-1500 parts of rhizoma dryopteris crassirhizomae, 5000-15000 parts of colza oil, 50-150 parts of hot pepper, 1000-3000 parts of green Chinese onions and 1-10 parts of brassin. The invention further relates to a preparation method and application of the green pollution-free plant compound preparation. The green pollution-free plant compound preparation is applied to preventing virus disease of the crops, the effect is novel and unique, and the products are green, environment-friendly, harmfulless and pollution-free.
Description
Technical field
The present invention relates to agriculture field, specifically, relating to a kind of green non-pollution plant compound formulation and its preparation method and application for preventing and treating crop virosis and fungi, bacteriosis.
Background technology
In the process of plant crop growth, the growth of the frequent damage to crops such as fungi, bacterium, causes serious injury the growth of crop.At present, conventional chemical agricultural chemicals, the requirement to people and animals and Environmental security can not be reached, due to plant virus obligate parasitism in plant cell, it copies required material, quantity, place are provided by host cell completely, and the immune system that plant does not have animal complete like that, the disease of plant virus is generally occurred, cause crops quality to decline, output reduces, and has had a strong impact on the income of peasant and the development of agricultural.4,000,000,000 U.S. dollars of the loss that the whole world is caused by the viroses of plant every year.
Summary of the invention
The object of this invention is to provide a kind of green non-pollution plant compound formulation for preventing and treating crop virosis and fungi, bacteriosis.
In order to realize object of the present invention, the invention provides a kind of green non-pollution plant compound formulation for preventing and treating crop virosis and fungi, bacteriosis, it is made up of the material of following weight portion: Momordica grosvenori 100-300 part, sealwort 50-200 part, Radix Astragali 50-200 part, coptis 50-200 part, root of Chinese wild ginger 500-1500 part, honeysuckle 1000-3000 part, rosin 1000-3000 part, ginkgo leaf 500-1500 part, thick wood-fern rhizome 500-1500 part, rapeseed oil 5000-15000 part, hot pepper 50-150 part, shallot 1000-3000 part and 1-10 part brassin.
Preferably, of the present invention for preventing and treating crop virosis and fungi, the green non-pollution plant compound formulation of bacteriosis is made up of the material of following weight portion: Momordica grosvenori 200 parts, sealwort 100 parts, the Radix Astragali 100 parts, the coptis 100 parts, the root of Chinese wild ginger 1000 parts, honeysuckle 2000 parts, rosin 2000 parts, ginkgo leaf 1000 parts, thick wood-fern rhizome 1000 parts, rapeseed oil 10000 parts, hot pepper 100 parts, 2000 parts, shallot and 4 parts of brassins.
More preferably, described compound formulation can be liquid preparation.
The present invention also provides a kind of preparation method of green non-pollution plant compound formulation for preventing and treating crop virosis and fungi, bacteriosis, and the method comprises the following steps:
1) compound concentration is the chitosan oligosaccharide sugar aqueous solution 500 kilograms of 1 % by weight;
2) 200 grams of Momordica grosvenoris are put in 50 DEG C of hot water of 100 kilograms, soak after four hours, filter and obtain filtrate;
3) by sealwort, the Radix Astragali, each 1000 grams of the coptis, be put in 50 DEG C of hot water of 100 kilograms, soak after four hours, filter and obtain filtrate;
4) 1000 grams of roots of Chinese wild ginger are put in the absolute ethyl alcohol of 10 kilograms, soak after four hours, filter and obtain filtrate;
5) 2000 grams of honeysuckles are put in 50 DEG C of hot water of 100 kilograms, soak four hours, filter and obtain filtrate;
6) 2000 grams of colophony powders are dissolved in 20 kilograms of absolute ethyl alcohols;
7), after 1000 grams of ginkgo leaves and 1000 grams of thick wood-fern rhizomes being soaked four hours with 20 kg ethanol, filter and obtain filtrate;
8) rapeseed oil of 10 kilograms is added in the Tween-80 of 20 kilograms, stir;
9) hot pepper of 100 grams is added water 5000 grams, heat 0.5 hour under the condition adding hot reflux, filter and obtain filtrate;
10) 2000 grams of shallots are added water 2000 grams, mash, filter and obtain filtrate;
11) by step 1) to 10) in obtained liquid put in 2000 liters of reactors together, add the brassin aqueous solution 1000 grams of 0.4 % by weight, mix and blend 20 minutes, add pure water 500 kilograms, stir after 40 minutes and leave standstill 2 hours, rear inspection bottling, vanning.
The present invention also provides composition for the preparation of control crop virosis and fungi, application in the green non-pollution plant compound formulation of bacteriosis, it is characterized in that, described composition is made up of the material of following weight portion: Momordica grosvenori 100-300 part, sealwort 50-200 part, Radix Astragali 50-200 part, coptis 50-200 part, root of Chinese wild ginger 500-1500 part, honeysuckle 1000-3000 part, rosin 1000-3000 part, ginkgo leaf 500-1500 part, thick wood-fern rhizome 500-1500 part, rapeseed oil 5000-15000 part, hot pepper 50-150 part, shallot 1000-3000 part and 1-10 part brassin.
Preferably, described composition is made up of the material of following weight portion: Momordica grosvenori 200 parts, sealwort 100 parts, the Radix Astragali 100 parts, the coptis 100 parts, the root of Chinese wild ginger 1000 parts, honeysuckle 2000 parts, rosin 2000 parts, ginkgo leaf 1000 parts, thick wood-fern rhizome 1000 parts, rapeseed oil 10000 parts, hot pepper 100 parts, 2000 parts, shallot and 4 parts of brassins.
More preferably, described compound formulation can be liquid preparation.
Chitosan oligosaccharide: can the immune system of stimulating plant, activate defense reaction, regulating plant produces antibacterial, antitoxin material, and the harm of inducing plant opposing fungi, bacterium and virus, can make fruits and vegetables, cereal crops, melon dish volume increase 10%-30%, improve product quality.
Momordica grosvenori: sugary, amino acid, flavonoids and fatty acid mixed also contain iodine, molybdenum and 26 kinds of trace elements.Flavonoids and fatty acid mixed all have very strong inhibitory action to virus.Polysaccharide, amino acid, iodine and trace element, except supply plant growth, and also have certain control action to virus.
The Radix Astragali: containing the various trace elements such as saponin, sucrose, polysaccharide, several amino acids, folic acid and selenium, zinc, copper, have strengthen plant immune function, antibacterial, antiviral, yellow over sixty years of age polysaccharide have and significantly strengthen crop cell immunologic function.
Sealwort: include Siberian solomonseal rhizome polysaccharide, has antivirus action.
The coptis: containing phenol and alkaloid, can press down malicious antibacterial desinsection.
The root of Chinese wild ginger: containing phenols and ketone compounds, antibacterial, antiviral and can pesticide-germicide.
Honeysuckle: be main antimicrobial component containing chlorogenic acid and isochlorogenic acid, antitoxin sterilization.
Colophony powder: pure natural pine tree output object, containing the acid of rosin acid anhydride, Kaempferol and antiviral substance.
Ginkgo leaf: have very strong inhibitory action to virus containing flavonoids.
Thick wood-fern rhizome: containing phenol, ketone compounds, desinsection also has stronger antivirus action.
Rapeseed oil: can prevent and treat virus disease containing fatty acid mixed, main component C13-C15 fatty acid mixt is antiviral derivant, simultaneously also containing phytohormone activity, can stimulate plant growth.
Brassin: be Nantural non-toxic plant growth regulator, photosynthesis of plant and resistance can be improved, Promoting plant growth, thus the generation and the development that reduce virus.
Capsicum: add hydro-thermal with sharp green pepper and boil, include capsaicine, can effectively prevent eliminating aphis, thus effectively can prevent and treat crop and encroach on infected plant virus by aphid.
Shallot: add water, mash and get liquid, all have control efficiency to various pests such as aphids, can prevent and treat because of aphid, thus can prevent and treat and encroach on the infected viroses of plant by aphid.
Tween-80 is surfactant, and second alcohol and water is solvent.
In proportion of crop planting process, plant virus is the second largest viroids being only second to fungi in plant virus, and plant virus can reach nearly 1000 kinds, and the characteristic of each viroids is not identical to the greatest extent, therefore multielement compound preparation must be adopted just to reach good control efficiency.Chitosan oligosaccharide, Momordica grosvenori, sealwort, Huang Qi, the coptis, honeysuckle, colophony powder, ginkgo leaf, rhizome of cyrtomium, rapeseed oil, capsicum, shallot, several raw material science combination, add ethanol, pure water, Tween-80 again, manufactured product, for preventing and treating crop virosis, have novel and unique effect, Product Green environmental protection, low toxicity are nuisanceless.
This preparation, by the application on apple tree, pear tree, peach, apricot, pawpaw, has all showed good control efficiency to virus disease.Working concentration is 500-1000 times of liquid, even spraying, after using this preparation, and various crop volume increase per mu 20-30%.This preparation passes through to tomato in green house, capsicum, cucumber, application on the crop such as muskmelon, watermelon, strawberry, leek, good to preventing and treating the equal expression effect of virus disease, working concentration is 500-1000 times of liquid, even spraying, uses rear various crop on average every mu of volume increase 20-30%.The use of this preparation on tobacco, also show respond well, working concentration is 500-1000 times of liquid, even spraying.Use rear tobacco leaf on average every mu of volume increase 15-20%.The application of this preparation on corn, potato is also cashed respond well, and working concentration is 500-1000 times of liquid, uses rear on average every mu of volume increase 20-30%.The application of this preparation on flowers, the control efficiency of all kinds of flowers is all 100%, and working concentration is 500-1000 times, even spraying.The feature that this product is different from other products is: nuisanceless, good to environment, instant effect, and control viral species wide spectrum, virus does not produce resistance to this agent.
This preparation is non-harmful product, environmentally safe, harmless to crop, fruit tree, vegetables.And grain, cotton and oil crop to spray after this preparation the control efficiency of virus disease more than 98%.After applying this product, can the income of user greatly, also bring great economic benefit to society simultaneously.
By in national some areas test of 3 years application, be used alone the rare 500-1000 of this product times liquid, evenly spray in susceptible crop, interval spray in four days once, effectively can control generation and the development of virus disease for general 2-3 days.This preparation can be mutually composite with foliage fertilizer, and can synergy.This preparation can with trace element, zinc sulphate, copper sulphate, mutually composite, and can synergy.This preparation can be mutually composite with moroxydine, Ningnanmycin, triacontanol, enadenine, and can synergy.
Embodiment
Below by way of the description of embodiment, the invention will be further described, but this is not limitation of the present invention, those skilled in the art are according to basic thought of the present invention, various amendment or improvement can be made, but only otherwise depart from basic thought of the present invention, all within the scope of the present invention.
The preparation of embodiment compound formulation of the present invention
1) compound concentration is the chitosan oligosaccharide sugar aqueous solution 500 kilograms of 1 % by weight;
2) 200 grams of Momordica grosvenoris are put in 50 DEG C of hot water of 100 kilograms, soak after four hours, filter and obtain filtrate;
3) by sealwort, the Radix Astragali, each 1000 grams of the coptis, be put in 50 DEG C of hot water of 100 kilograms, soak after four hours, filter and obtain filtrate;
4) 1000 grams of roots of Chinese wild ginger are put in the absolute ethyl alcohol of 10 kilograms, soak after four hours, filter and obtain filtrate;
5) 2000 grams of honeysuckles are put in 50 DEG C of hot water of 100 kilograms, soak four hours, filter and obtain filtrate;
6) 2000 grams of colophony powders are dissolved in 20 kilograms of absolute ethyl alcohols;
7), after 1000 grams of ginkgo leaves and 1000 grams of thick wood-fern rhizomes being soaked four hours with 20 kg ethanol, filter and obtain filtrate;
8) rapeseed oil of 10 kilograms is added in the Tween-80 of 20 kilograms, stir;
9) hot pepper of 100 grams is added water 5000 grams, heat 0.5 hour under the condition adding hot reflux, filter and obtain filtrate;
10) 2000 grams of shallots are added water 2000 grams, mash, filter and obtain filtrate;
11) by step 1) to 10) in obtained liquid put in 2000 liters of reactors together, add the brassin aqueous solution 1000 grams of 0.4 % by weight, mix and blend 20 minutes, add pure water 500 kilograms, stir after 40 minutes and leave standstill 2 hours, to obtain final product.
Experimental example
Filter paper enzyme classical in this area is adopted to carry out bacteriostatic test, wherein:
1. material
1.1 for examination bacterial classification
Micrococcus luteus ATCC 9341 and bacillus subtilis ATCC 6633, purchased from Central Plains, Beijing company.
1.2 culture fluid
Nutrient agar and nutrient broth, purchased from Chen Yu experimental facilities Co., Ltd of BeiJing ZhongKe.
1.3 test method
Micrococcus luteus ATCC 9341 and bacillus subtilis ATCC 6633 is inoculated in agar plate nutrient solution plane, evenly gathers during inoculation.The aseptic circular filter paper sheet of tweezer, sprays the compound formulation prepared in embodiment respectively, to complete wetting, is attached in the agar plate nutrient solution plane of inoculated bacteria.Agar plate is placed in the incubator of 37 DEG C, incubation 4 hours.Measure the diameter of bacterial restrain.Measure and average for 3 times.
Found that, for micrococcus luteus ATCC 9341, the diameter of bacterial restrain is 13.5cm, and for bacillus subtilis ATCC 6633, the diameter of bacterial restrain is 12.9cm.
Claims (7)
1. one kind for preventing and treating the green non-pollution plant compound formulation of crop virosis and fungi, bacteriosis, it is characterized in that, it is made up of the material of following weight portion: Momordica grosvenori 100-300 part, sealwort 50-200 part, Radix Astragali 50-200 part, coptis 50-200 part, root of Chinese wild ginger 500-1500 part, honeysuckle 1000-3000 part, rosin 1000-3000 part, ginkgo leaf 500-1500 part, thick wood-fern rhizome 500-1500 part, rapeseed oil 5000-15000 part, hot pepper 50-150 part, shallot 1000-3000 part and 1-10 part brassin.
2. the green non-pollution plant compound formulation for preventing and treating crop virosis and fungi, bacteriosis according to claim 1, it is characterized in that, it is made up of the material of following weight portion: Momordica grosvenori 200 parts, sealwort 100 parts, the Radix Astragali 100 parts, the coptis 100 parts, the root of Chinese wild ginger 1000 parts, honeysuckle 2000 parts, rosin 2000 parts, ginkgo leaf 1000 parts, thick wood-fern rhizome 1000 parts, rapeseed oil 10000 parts, hot pepper 100 parts, 2000 parts, shallot and 4 parts of brassins.
3. the green non-pollution plant compound formulation for preventing and treating crop virosis and fungi, bacteriosis according to claim 1 and 2, it is characterized in that, described compound formulation can be liquid preparation.
4., for preventing and treating the preparation method of green non-pollution plant compound formulation for crop virosis and fungi, bacteriosis, the method comprises the following steps:
1) compound concentration is the chitosan oligosaccharide sugar aqueous solution 500 kilograms of 1 % by weight;
2) 200 grams of Momordica grosvenoris are put in 50 DEG C of hot water of 100 kilograms, soak after four hours, filter and obtain filtrate;
3) by sealwort, the Radix Astragali, each 1000 grams of the coptis, be put in 50 DEG C of hot water of 100 kilograms, soak after four hours, filter and obtain filtrate;
4) 1000 grams of roots of Chinese wild ginger are put in the absolute ethyl alcohol of 10 kilograms, soak after four hours, filter and obtain filtrate;
5) 2000 grams of honeysuckles are put in 50 DEG C of hot water of 100 kilograms, soak four hours, filter and obtain filtrate;
6) 2000 grams of colophony powders are dissolved in 20 kilograms of absolute ethyl alcohols;
7), after 1000 grams of ginkgo leaves and 1000 grams of thick wood-fern rhizomes being soaked four hours with 20 kg ethanol, filter and obtain filtrate;
8) rapeseed oil of 10 kilograms is added in the Tween-80 of 20 kilograms, stir;
9) hot pepper of 100 grams is added water 5000 grams, heat 0.5 hour under the condition adding hot reflux, filter and obtain filtrate;
10) 2000 grams of shallots are added water 2000 grams, mash, filter and obtain filtrate;
11) by step 1) to 10) in obtained liquid put in 2000 liters of reactors together, add the brassin aqueous solution 1000 grams of 0.4 % by weight, mix and blend 20 minutes, add pure water 500 kilograms, stir after 40 minutes and leave standstill 2 hours, rear inspection bottling, vanning.
5. composition for the preparation of control crop virosis and fungi, bacteriosis green non-pollution plant compound formulation in application, it is characterized in that, described composition is made up of the material of following weight portion: Momordica grosvenori 100-300 part, sealwort 50-200 part, Radix Astragali 50-200 part, coptis 50-200 part, root of Chinese wild ginger 500-1500 part, honeysuckle 1000-3000 part, rosin 1000-3000 part, ginkgo leaf 500-1500 part, thick wood-fern rhizome 500-1500 part, rapeseed oil 5000-15000 part, hot pepper 50-150 part, shallot 1000-3000 part and 1-10 part brassin.
6. application according to claim 5, it is characterized in that, described composition is made up of the material of following weight portion: Momordica grosvenori 200 parts, sealwort 100 parts, the Radix Astragali 100 parts, the coptis 100 parts, the root of Chinese wild ginger 1000 parts, honeysuckle 2000 parts, rosin 2000 parts, ginkgo leaf 1000 parts, thick wood-fern rhizome 1000 parts, rapeseed oil 10000 parts, hot pepper 100 parts, 2000 parts, shallot and 4 parts of brassins.
7. the application according to claim 5 or 6, is characterized in that, described compound formulation can be liquid preparation.
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Cited By (8)
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| CN105028506A (en) * | 2015-06-27 | 2015-11-11 | 蚌埠市双墩农业生物科技开发有限责任公司 | Composition for preventing and controlling leaf spot disease of pumpkin |
| CN105394093A (en) * | 2015-12-22 | 2016-03-16 | 关慧明 | Moroxydine hydrochloride vegetable crop virus disease prevention and control pesticide |
| CN105815343A (en) * | 2016-04-12 | 2016-08-03 | 安徽林海园林绿化股份有限公司 | Michelia chapensis seedling damping-off preventing and treating agent |
| CN106305802A (en) * | 2016-08-19 | 2017-01-11 | 北海市伟恒生态农业有限公司 | Fertilizer for preventing banana mosaic heart rot |
| CN107980496A (en) * | 2017-12-04 | 2018-05-04 | 江西宋氏灵斛生物科技有限公司 | A kind of implantation methods of dendrobium candidum |
| CN108041095A (en) * | 2017-12-08 | 2018-05-18 | 柳州雅瑞科技有限公司 | A kind of botanical fungicide for preventing edible lily virosis and preparation method thereof |
| JP7109827B1 (en) | 2021-11-16 | 2022-08-01 | 株式会社ハニック・ホワイトラボ | antiviral agent |
| CN115299459A (en) * | 2022-07-26 | 2022-11-08 | 云南省农业科学院农业环境资源研究所 | Composition for preventing and treating tobacco virus diseases and application thereof |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105028506A (en) * | 2015-06-27 | 2015-11-11 | 蚌埠市双墩农业生物科技开发有限责任公司 | Composition for preventing and controlling leaf spot disease of pumpkin |
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| CN105815343A (en) * | 2016-04-12 | 2016-08-03 | 安徽林海园林绿化股份有限公司 | Michelia chapensis seedling damping-off preventing and treating agent |
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| CN108041095A (en) * | 2017-12-08 | 2018-05-18 | 柳州雅瑞科技有限公司 | A kind of botanical fungicide for preventing edible lily virosis and preparation method thereof |
| JP7109827B1 (en) | 2021-11-16 | 2022-08-01 | 株式会社ハニック・ホワイトラボ | antiviral agent |
| JP2023073719A (en) * | 2021-11-16 | 2023-05-26 | 株式会社ハニック・ホワイトラボ | antiviral agent |
| CN115299459A (en) * | 2022-07-26 | 2022-11-08 | 云南省农业科学院农业环境资源研究所 | Composition for preventing and treating tobacco virus diseases and application thereof |
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Application publication date: 20150422 |