CN104512859A - Manufacturing and integrating method of multichannel high-efficient biological sensor - Google Patents

Abstract

The invention provides a manufacturing and integrating method of a multichannel high-efficient biological sensor. The method is characterized by comprising following steps: (1) preparing silicon nanowires; (2) preparing gold nanoparticles; (3) decorating the silicon nanowires with the gold nanoparticles; and (4) finally connecting a wire onto a silicon chip through electric-conductive silver slurry and epoxy resin to manufacture the biological sensor based on nano silicon. The method is simple and can manufacture the biological sensor in large scale without one-by-one production in the prior art, thereby increasing production efficiency and meanwhile reducing production cost. In the multichannel high-efficient biological sensor, specificity and biocompatibility among nano gold wires, nano gold particles and DNA are cleverly utilized, so that the method is easy to carry out and has a wide applicability.

Description

The making integrated approach of multichannel high-efficient biology sensor

Technical field

the present invention relates to biological field, particularly relate to a kind of making integrated approach of multichannel high-efficient biology sensor.

Background technology

Biology sensor is the sensor of a class special shape, is made up of biomolecule recognition component and all kinds of physics, chemical transducer, for the analysis and resolution of various living matter and chemical substance.Biology sensor melts biology, chemistry, physics, information science and correlation technique in one, has developed into a very active research field.Biology sensor (biosensor) is responsive to biological substance and its concentration is converted to the instrument that the signal of telecommunication detects, and is to make analysis tool that recognition component bioactivators such as () such as enzyme, antibody, antigen, microorganism, cell, tissue, nucleic acid and suitable physics and chemistry transducer (as oxygen electrode, photosensitive tube, FET, piezo-electric crystal etc.) and signal amplifying apparatus form or system by immobilized biological sensitive materials.Biology sensor has the function accepted with conversion.But biology sensor manufacture craft is now numerous and diverse, and can only singlely produce during production, production cost is high.

Summary of the invention

According to above technical problem, the invention provides a kind of making integrated approach of multichannel high-efficient biology sensor, it is characterized in that the method comprises following concrete steps:

1) preparation of silicon nanowires

First silicon chip is put into cleaning solution to clean, acetone soln ultrasonic cleaning 3-5 minute, then absolute ethyl alcohol ultrasonic cleaning 3-5 minute, 4 times repeatedly, front and back, reusable heat, cold deionization solution rinse, after drying up, and dry for standby;

2) preparation of golden nanometer particle

In a heated condition, be n(reducing agent respectively to adding PVP protective agent and not adding admixture mass concentration ratio in the protectant standard chlorauric acid solution of PVP) reducing agent of Bn (AU)=1B1,2B1,4B1, generate mauve solution, namely obtain golden nanometer particle colloidal sol;

3) silicon nanowires is decorated with golden nanometer particle

Later silicon nanowires is put into the ethanolic solution being added with silane coupler 3-aminopropyl trimethoxy alkane, the volume ratio of silane coupler and ethanol is 1:1000, ethanolic solution is heated to boiling, time 40-45 minute, take out silicon chip, rinse with ethanol, then wash ethanol off by deionized water; This silicon chip is put into solution of gold nanoparticles to soak 12 hours, clean by deionized water after taking-up, then put into drying box and carry out drying;

4) finally with conductive silver paste and epoxy resin, wire is connected on silicon chip, the obtained biology sensor based on silicon nanometer.

Beneficial effect of the present invention is: preparation method of the present invention is simple, can produce in enormous quantities, need not produce one by one as before, improve production efficiency and reduce production cost simultaneously; Biology sensor prepared by the present invention applies gold nanowire, golden nanometer particle and DNA specificity each other and biocompatibility cleverly, is easy to realize, and has applicability widely.

Detailed description of the invention

According to following examples, the present invention is further described:

Preparation method is as follows;

First silicon chip is put into cleaning solution to clean, acetone soln ultrasonic cleaning 3-5 minute, then absolute ethyl alcohol ultrasonic cleaning 3-5 minute, 4 times repeatedly, front and back, reusable heat, cold deionization solution rinse, after drying up, and dry for standby;

Then in a heated condition, be n(reducing agent respectively to adding PVP protective agent and not adding admixture mass concentration ratio in the protectant standard chlorauric acid solution of PVP) reducing agent of Bn (AU)=1B1,2B1,4B1, generate mauve solution, namely obtain golden nanometer particle colloidal sol;

Again later silicon nanowires is put into the ethanolic solution being added with silane coupler 3-aminopropyl trimethoxy alkane, the volume ratio of silane coupler and ethanol is 1:1000, ethanolic solution is heated to boiling, time 40-45 minute, take out silicon chip, rinse with ethanol, then wash ethanol off by deionized water; This silicon chip is put into solution of gold nanoparticles to soak 12 hours, clean by deionized water after taking-up, then put into drying box and carry out drying;

Finally with conductive silver paste and epoxy resin, wire is connected on silicon chip, the obtained biology sensor based on silicon nanometer.

The above is only the preferred embodiment of the present invention, it should be pointed out that for those skilled in the art, under the premise without departing from the principles of the invention, can also make some improvement, and these improvement also should be considered as protection scope of the present invention.

Claims (1)

1. a making integrated approach for multichannel high-efficient biology sensor, is characterized in that the method comprises following concrete steps:

1) preparation of silicon nanowires

First silicon chip is put into cleaning solution to clean, acetone soln ultrasonic cleaning 3-5 minute, then absolute ethyl alcohol ultrasonic cleaning 3-5 minute, 4 times repeatedly, front and back, reusable heat, cold deionization solution rinse, after drying up, and dry for standby;

2) preparation of golden nanometer particle

In a heated condition, be n(reducing agent respectively to adding PVP protective agent and not adding admixture mass concentration ratio in the protectant standard chlorauric acid solution of PVP) reducing agent of Bn (AU)=1B1,2B1,4B1, generate mauve solution, namely obtain golden nanometer particle colloidal sol;

3) silicon nanowires is decorated with golden nanometer particle

Later silicon nanowires is put into the ethanolic solution being added with silane coupler 3-aminopropyl trimethoxy alkane, the volume ratio of silane coupler and ethanol is 1:1000, ethanolic solution is heated to boiling, time 40-45 minute, take out silicon chip, rinse with ethanol, then wash ethanol off by deionized water; This silicon chip is put into solution of gold nanoparticles to soak 12 hours, clean by deionized water after taking-up, then put into drying box and carry out drying;

4) finally with conductive silver paste and epoxy resin, wire is connected on silicon chip, the obtained biology sensor based on silicon nanometer.