CN104488829A - Culture method of moina organism food for aquaculture - Google Patents
Culture method of moina organism food for aquaculture Download PDFInfo
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- CN104488829A CN104488829A CN201510007369.XA CN201510007369A CN104488829A CN 104488829 A CN104488829 A CN 104488829A CN 201510007369 A CN201510007369 A CN 201510007369A CN 104488829 A CN104488829 A CN 104488829A
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- 241001302187 Moina Species 0.000 title claims abstract description 67
- 238000009360 aquaculture Methods 0.000 title claims abstract description 11
- 244000144974 aquaculture Species 0.000 title claims abstract description 11
- 235000013305 food Nutrition 0.000 title abstract description 9
- 238000012136 culture method Methods 0.000 title abstract 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 37
- 238000009395 breeding Methods 0.000 claims abstract description 31
- 230000001488 breeding effect Effects 0.000 claims abstract description 31
- 241000894006 Bacteria Species 0.000 claims abstract description 11
- 239000007788 liquid Substances 0.000 claims abstract description 9
- 235000007091 Chlorella pyrenoidosa Nutrition 0.000 claims abstract description 6
- 241000195652 Auxenochlorella pyrenoidosa Species 0.000 claims abstract 2
- 239000012531 culture fluid Substances 0.000 claims description 13
- 238000000034 method Methods 0.000 claims description 13
- 239000012141 concentrate Substances 0.000 claims description 8
- ATJFFYVFTNAWJD-UHFFFAOYSA-N Tin Chemical compound [Sn] ATJFFYVFTNAWJD-UHFFFAOYSA-N 0.000 claims description 5
- 230000004913 activation Effects 0.000 claims description 4
- 241000003595 Aurantiochytrium limacinum Species 0.000 claims description 3
- 239000006260 foam Substances 0.000 claims description 3
- 230000008014 freezing Effects 0.000 claims description 3
- 238000007710 freezing Methods 0.000 claims description 3
- 230000000243 photosynthetic effect Effects 0.000 claims description 3
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 2
- 238000004140 cleaning Methods 0.000 claims description 2
- 238000001914 filtration Methods 0.000 claims 1
- 238000007599 discharging Methods 0.000 abstract 1
- 230000001105 regulatory effect Effects 0.000 abstract 1
- 239000008399 tap water Substances 0.000 abstract 1
- 235000020679 tap water Nutrition 0.000 abstract 1
- 241001247197 Cephalocarida Species 0.000 description 9
- 238000012360 testing method Methods 0.000 description 9
- 241000195649 Chlorella <Chlorellales> Species 0.000 description 5
- 230000037396 body weight Effects 0.000 description 5
- 230000004083 survival effect Effects 0.000 description 5
- 235000019786 weight gain Nutrition 0.000 description 5
- 244000249214 Chlorella pyrenoidosa Species 0.000 description 4
- 241000238571 Cladocera Species 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- 241000251468 Actinopterygii Species 0.000 description 3
- 241000239250 Copepoda Species 0.000 description 3
- 239000000796 flavoring agent Substances 0.000 description 3
- 235000019634 flavors Nutrition 0.000 description 3
- 239000012535 impurity Substances 0.000 description 3
- 230000000050 nutritive effect Effects 0.000 description 3
- 241000133262 Nauplius Species 0.000 description 2
- 241000861914 Plecoglossus altivelis Species 0.000 description 2
- 241001441722 Takifugu rubripes Species 0.000 description 2
- 241000607598 Vibrio Species 0.000 description 2
- 206010047400 Vibrio infections Diseases 0.000 description 2
- 230000000366 juvenile effect Effects 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 230000035764 nutrition Effects 0.000 description 2
- 235000019629 palatability Nutrition 0.000 description 2
- 235000020995 raw meat Nutrition 0.000 description 2
- 230000000384 rearing effect Effects 0.000 description 2
- 231100000033 toxigenic Toxicity 0.000 description 2
- 230000001551 toxigenic effect Effects 0.000 description 2
- 235000001674 Agaricus brunnescens Nutrition 0.000 description 1
- 244000025254 Cannabis sativa Species 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 102000002322 Egg Proteins Human genes 0.000 description 1
- 108010000912 Egg Proteins Proteins 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- 241001628273 Moina macrocopa Species 0.000 description 1
- 241000700141 Rotifera Species 0.000 description 1
- 241000607142 Salmonella Species 0.000 description 1
- 241000607768 Shigella Species 0.000 description 1
- 241000191967 Staphylococcus aureus Species 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- NIXOWILDQLNWCW-UHFFFAOYSA-N acrylic acid group Chemical group C(C=C)(=O)O NIXOWILDQLNWCW-UHFFFAOYSA-N 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 235000019789 appetite Nutrition 0.000 description 1
- 230000036528 appetite Effects 0.000 description 1
- 229910052785 arsenic Inorganic materials 0.000 description 1
- RQNWIZPPADIBDY-UHFFFAOYSA-N arsenic atom Chemical compound [As] RQNWIZPPADIBDY-UHFFFAOYSA-N 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 239000004568 cement Substances 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 230000003031 feeding effect Effects 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 239000013505 freshwater Substances 0.000 description 1
- 229910002804 graphite Inorganic materials 0.000 description 1
- 239000010439 graphite Substances 0.000 description 1
- -1 growth instability Substances 0.000 description 1
- 230000012447 hatching Effects 0.000 description 1
- 230000008676 import Effects 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000001418 larval effect Effects 0.000 description 1
- 239000002932 luster Substances 0.000 description 1
- QSHDDOUJBYECFT-UHFFFAOYSA-N mercury Chemical compound [Hg] QSHDDOUJBYECFT-UHFFFAOYSA-N 0.000 description 1
- 229910052753 mercury Inorganic materials 0.000 description 1
- 239000008239 natural water Substances 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 210000004681 ovum Anatomy 0.000 description 1
- 235000013406 prebiotics Nutrition 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Fodder In General (AREA)
- Feed For Specific Animals (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention provides a culture method of a moina organism food for aquaculture. The culture method comprises the steps of adding tap water to a breeding pool and a culture pool; regulating the temperature to be 25 to 30 DEG C; charging compressed air; feeding chlorella pyrenoidosa condensate and EM bacterium liquid into a food tank; charging compressed air to activate for 24 to 48 hours under the temperature of 25 to 30 DEG C to obtain a mixed food; feeding the mixed food which is 1 to 5 thousandths of the mass of water into the breeding pool; feeding young moina which is 1 to 3 thousandths of the mass of water into the breeding pool; continuously adding the mixed food to the breeding pool by a flowing manner; transferring moina from the breeding pool into the culture pool after 48 to 72h of culture; keeping the temperature of the culture pool to be 25 to 30 DEG C; discharging and picking when moina in the culture pool is more than 10 thousandths of the mass of the culture water. The moina organism food is the most ideal moina organism initial food of the aquaculture industry in the future.
Description
Technical field
The present invention relates to a kind of cultural method of the Moina biological feed for aquaculture, belong to biological technical field.
Background technology
In the seed rearing of aquaculture, the biological feed after wheel animalcule that natural world finds has artemia larvae, copepoda and cladocera (i.e. Moina).Artemia larvae (being formed by artemia ovum hatching) because of resource day by day rare, price rises violently year by year, and there is the pathogenic bacteria such as vibrios and pollute; Though copepoda, cladocera, with the nutrition abundant for bait has of micro-algae, are fished for by the restriction such as season, weather by natural water area, originate very unstable.So rely on sufficient micro-algae biological, become the dream of culture fishery indoor high-density cultivation copepoda and cladocera in conjunction with prebiotic mushroom.
Moina (Moina macrocopa), also known as cladocera, belong to invertebrate, the long 0.2-l.0mm of body, majority is grown in freshwater, lake, rivers, is the important bait of fish, therefore is commonly called as " worm for fishing ".The wide adaptability of Moina, fertility is strong, and growth rapidly, and is of high nutritive value, and is the agreeable to the taste bait in aquaculture larval rearing stage.Moina indoor high-density is cultivated, overcome the defect such as field self-sow environment living contaminants, growth instability, product production capacity is high, nutrition is agreeable to the taste, clean nothing is dirty, easy to use, and being the excellent substitute of artemia larvae, is the optimal biological open-mouthed bait of following culture fishery.
Summary of the invention
In view of background technology Problems existing, the invention provides a kind of cultural method of the Moina biological feed for aquaculture, its can turn out have toxigenic capacity low, be of high nutritive value, biological feed that palatability is strong, pollution-free, easy to use.
In order to realize object of the present invention, the invention provides a kind of cultural method of the Moina biological feed for aquaculture, this cultural method comprises step: joined by running water in breeding pond and culture pond, temperature is adjusted to 25-30 DEG C, and passing into compressed air, throughput is advisable with the unhurried current forming water body 3-5cm/s; Chlorella pyrenoidosa concentrate and EM bacterium liquid are put in bait tin according to the ratio that volume ratio is 1:1, at the temperature of 25-30 DEG C, passes into compressed air activation 24-48h, form the mixed feed of Moina; Joined by mixed feed in breeding pond, addition is the 1-5 ‰ cultivating Water quality; Put into by the seedling of Moina in breeding pond, input amount is the 1-3 ‰ of breeding pond Water quality, and adds mixed feed to the relaying afterflow of breeding pond; After the Moina in breeding pond cultivates 48-72h, render in culture pond by culture fluid together with Moina, culture pond temperature keeps 25-30 DEG C; When Moina in culture pond is cultivate Water quality more than 10 ‰, Moina polypide and culture fluid are discharged from the overfall of culture pond.
By above technical scheme, beneficial effect of the present invention is as follows: have compared with traditional bait such as artemia larvae toxigenic capacity low, be of high nutritive value, palatability is strong, the feature such as pollution-free, easy to use, is the optimal biological open-mouthed bait of following culture fishery.
Embodiment
Be further described the specific embodiment of the present invention below in conjunction with embodiment, advantage and disadvantage of the present invention will be more clear along with description.But these embodiments are only exemplary, do not form any restriction to scope of the present invention.
The invention provides a kind of cultural method of the Moina biological feed for aquaculture, this cultural method comprises step: joined by running water in breeding pond and culture pond, temperature is adjusted to 25-30 DEG C, and passes into compressed air, and throughput is advisable with the unhurried current forming water body 3-5cm/s; Chlorella pyrenoidosa concentrate and EM bacterium liquid are put in bait tin according to the ratio that volume ratio is 1:1, at the temperature of 25-30 DEG C, passes into compressed air activation 24-48h, form the mixed feed of Moina; Joined by mixed feed in breeding pond, addition is the 1-5 ‰ cultivating Water quality; Put into by the seedling of Moina in breeding pond, input amount is the 1-3 ‰ of breeding pond Water quality, and continues to add mixed feed in breeding pond; After the Moina in breeding pond cultivates 48-72h, render in culture pond by culture fluid together with Moina, culture pond temperature keeps 25-30 DEG C; When Moina in culture pond is cultivate Water quality more than 10 ‰, Moina polypide and culture fluid are discharged from the overfall of culture pond.
Wherein, EM bacterium liquid comprises hay bacillus, photosynthetic bacteria and schizochytrium limacinum.
Wherein, the PH of culture fluid is 6.5-8.0.
Wherein also comprise step:
The Moina polypide that overfall is discharged is through 80-100 object sieves; The Moina filtered out, after running water embathes, is filled in plastic sack, lies in refrigerator tray, then IQF with the thickness of 1-2cm, and quick freezing temperature controls below-30 DEG C; The Moina polypide vanning freezed is deposited, and storage temperature is subzero 15-20 DEG C.
Filter the culture fluid obtained after foam separator purified treatment, return the use of culture pond cycle.
Inventionculture tools and facility
Cultivate utensil: Moina High Density Cultivation uses breeding pond and large-scale culture pond two kinds of containers, breeding pond adopts acrylic water vat, for cultivation and the amplification of Moina seedling, is generally 1-2m
3; Culture pond adopts cement pit, and cultivate for industrialization, volume is 10-20m
3.
Cultivate water body: concrete water level is determined depending on Moina seedling quantity, between the 30-40% caning be controlled in capacity in advance, add to gradually afterwards pond dark 80%.
Cultivate bait: the cultivation bait of mixed liquor as Moina utilizing chlorella pyrenoidosa concentrate and probio (containing withered grass bacterium, photosynthetic bacteria, schizochytrium limacinum).
Hot-water boiler: for culture pond provides thermal source, utilizes circulating hot water to meet the thermostatic control of Moina culture pond by heat exchanging pipe.
Bait tin and measuring pump: for equably for Moina culture pond provides bait, feeding rate is determined according to the density of Moina, and daily ration, feeding quantity should control, in the appetite more than needed meeting Moina 12-24 hour all the time, to avoid lacking food and excessive residual bait pollution.
Foam separator: for removing the organic matter in culture fluid, to reduce the Organic Pollutions such as polypide ight soil, maintains water cleaning.
Embodiment 1
Joined by running water in breeding pond and culture pond, temperature is adjusted to 28 DEG C, and passes into compressed air, and throughput is advisable with the unhurried current forming water body 4cm/s; Chlorella pyrenoidosa concentrate and EM bacterium liquid are put in bait tin according to the ratio that volume ratio is 1:1, at the temperature of 28 DEG C, passes into compressed air activation 36h, form the mixed feed of Moina; Joined by mixed feed in breeding pond, addition is cultivate Water quality 3 ‰; Put into by the seedling of Moina in breeding pond, input amount is 4.6 ‰ of breeding pond Water quality, and continues to add mixed feed in breeding pond; After the Moina in breeding pond cultivates 72h, render in culture pond by culture fluid together with Moina, culture pond temperature keeps 25-30 DEG C; When Moina in culture pond is cultivate Water quality more than 10 ‰, Moina polypide and culture fluid are discharged from the overfall of culture pond.
Condition of culture: the water yield: 10L; Water temperature: 28.0 DEG C; Incubation time: 72 hours.
Cultivation results: throw in Moina seedling 460g, account for 4.6% of water body, can output be expected: 46kg/T; Feed coefficient is 1:1.
Embodiment 2
All the other are with embodiment 1
Condition of culture: water yield 1000L, water temperature 25-26 DEG C; Cultivation time 48h.
Result of the test: throw in Moina seedling 3kg, output 21kg, net production 18kg, consume chlorella concentrate 30kg, feed coefficient (mass ratio of net production and chlorella concentrate) is 1:1.7.
Embodiment 3
All the other are with embodiment 1
Condition of culture: water yield 20000L, water temperature 25-26 DEG C; Cultivation time 54h.
Result of the test: throw in Moina seedling 50kg, output 318kg, net production 268kg, consume chlorella concentrate 500kg, feed coefficient is 1:1.87.
1 embodiment 1-3 testing result:
Sense environmental conditions: temperature 19 DEG C of relative moisture 45%
Detect instrument: EK-200i electronic balance AA600 sampling Graphite Furnace Atomic Absorption device
LRH-150 biochemical cultivation case
The testing result of Moina biological feed prepared by table 1 embodiment 1-3
| Project | Embodiment 1 | Embodiment 2 | Embodiment 3 | Evaluation index |
| Color and luster | Bronzing | Bronzing | Bronzing | Bronzing |
| Flavour and smell | Tool raw meat delicate flavour, free from extraneous odour | With embodiment 1 | With embodiment 1 | Tool raw meat delicate flavour, free from extraneous odour |
| Shape | Paste liquid or ice cube, polypide is intact | With embodiment 1 | With embodiment 1 | Paste liquid or ice cube, polypide is intact |
| Impurity | Without macroscopic exogenous impurity | With embodiment 1 | With embodiment 1 | Without macroscopic exogenous impurity |
| Protein (g/100g) | 6.8 | 6.5 | 6.4 | ≥5.0 |
| Total arsenic (mg/kg) | 0.2 | 0.2 | 0.2 | ≤1 |
| Plumbous (mg/kg) | 0.1 | 0.18 | 0.2 | ≤0.5 |
| Mercury (mg/kg) | 0.01 | 0.01 | 0.01 | ≤0.05 |
| Total plate count (cfu/g) | 2.4*10 4 | 3.2*10 4 | 2.8*10 4 | ≤50000 |
| Escherichia coli (MPN/100g) | Do not detect | Do not detect | Do not detect | ≤90 |
| Shigella | Do not detect | Do not detect | Do not detect | Must not detect |
| Salmonella | Do not detect | Do not detect | Do not detect | Must not detect |
| Staphylococcus aureus | Do not detect | Do not detect | Do not detect | Must not detect |
| Secondary dissolubility vibrios | Do not detect | Do not detect | Do not detect | Must not detect |
As seen from Table 1, the Moina using the cultural method of Moina bait of the present invention to obtain all meets Q/DZ 0002-2014 Moina biological feed company standard.
2, the comparative test of Moina biological feed and traditional bait result of use
Test 1:2009 year February in December to 2010, adopt the 3 kinds of bait examinations such as Moina, artemia nauplii, import " the first under heaven " board manufactured feed through chlorella of feeding to support sweetfish (Plecoglossus altivelis) young, juvenile fish, carry out the comparison of survival rate and growth rate.8 weeks test periods, result shows: the survival rate of 3 kinds of bait is identical, is 100%.The growth result of Moina of feeding is best, and body weight gain rate is 1882.84%; The body weight gain rate of feeding artemia nauplius is 363.24%; The body weight gain rate of manufactured feed of feeding is 493.14%.
Test 2:2010 year May in March to 2010, adopt and support Fugu rubripes (Takifugu rubripes) young, juvenile fish through the Moina of chlorella of feeding, artemia nauplii 2 kinds of bait examinations, carry out survival rate and growth rate compares.Test 6 weeks by a definite date, result shows: the feeding effect of Moina is best, and survival rate is 86%, and body weight gain rate is 1680%; The survival rate of feeding artemia nauplius is 51%, and body weight gain rate is 820%.
It will be understood by those skilled in the art that and can modify to the details of technical solution of the present invention and form or replace down without departing from the spirit and scope of the present invention, but these amendments and replacement all fall within the scope of protection of the present invention.
Claims (5)
1., for a cultural method for the Moina biological feed of aquaculture, it is characterized in that, comprise step:
Joined by running water in breeding pond and culture pond, temperature is adjusted to 25-30 DEG C, and passes into compressed air, and throughput is advisable with the unhurried current forming water body 3-5cm/s;
Chlorella pyrenoidosa concentrate and EM bacterium liquid are put in bait tin according to the ratio that volume ratio is 1:1, at the temperature of 25-30 DEG C, passes into compressed air activation 24-48h, form the mixed feed of Moina;
Joined by mixed feed in breeding pond, addition is the 1-5 ‰ cultivating Water quality;
Put into by the seedling of Moina in breeding pond, input amount is the 1-3 ‰ of breeding pond Water quality, and adds mixed feed to the relaying afterflow of breeding pond;
After the Moina in breeding pond cultivates 48-72h, render in culture pond by culture fluid together with Moina, culture pond temperature keeps 25-30 DEG C;
When Moina in culture pond is cultivate Water quality more than 10 ‰, Moina polypide and culture fluid are discharged from the overfall of culture pond.
2. cultural method according to claim 1, is characterized in that, EM bacterium liquid comprises hay bacillus, photosynthetic bacteria and schizochytrium limacinum.
3. cultural method according to claim 1, is characterized in that,
The PH of culture fluid is 6.5-8.0.
4. cultural method according to claim 1, is characterized in that, also comprises step:
The Moina polypide that overfall is discharged is filtered through 80-100 object sieves and gathers;
By the Moina polypide cleaning filtered out, freezing below-30 DEG C;
Deposit in the freezer of-15 DEG C to-20 DEG C through freezing Moina polypide.
5. cultural method according to claim 1, is characterized in that,
The culture fluid that filtration obtains returns culture pond and recycles after foam separator purified treatment.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510007369.XA CN104488829B (en) | 2015-01-07 | 2015-01-07 | A kind of cultural method of the naked abdomen biological feed for aquaculture |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510007369.XA CN104488829B (en) | 2015-01-07 | 2015-01-07 | A kind of cultural method of the naked abdomen biological feed for aquaculture |
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| Publication Number | Publication Date |
|---|---|
| CN104488829A true CN104488829A (en) | 2015-04-08 |
| CN104488829B CN104488829B (en) | 2016-07-06 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105284740A (en) * | 2015-10-27 | 2016-02-03 | 长江大学 | Method for quickly identifying sex of young daphnia of daphnia magna |
| CN107751057A (en) * | 2017-10-17 | 2018-03-06 | 广东工业大学 | A kind of Daphnia magna food chain type single cylinder conservation cultural method |
| CN108184767A (en) * | 2018-02-28 | 2018-06-22 | 王景春 | A kind of method of cowboying |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105284740A (en) * | 2015-10-27 | 2016-02-03 | 长江大学 | Method for quickly identifying sex of young daphnia of daphnia magna |
| CN105284740B (en) * | 2015-10-27 | 2018-03-02 | 长江大学 | Quick discriminating Daphnia magna children's Magna method for distinguishing |
| CN107751057A (en) * | 2017-10-17 | 2018-03-06 | 广东工业大学 | A kind of Daphnia magna food chain type single cylinder conservation cultural method |
| CN107751057B (en) * | 2017-10-17 | 2021-02-09 | 广东工业大学 | Chain type single-cylinder seed-preserving culture method for daphnia magna food |
| CN108184767A (en) * | 2018-02-28 | 2018-06-22 | 王景春 | A kind of method of cowboying |
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| Publication number | Publication date |
|---|---|
| CN104488829B (en) | 2016-07-06 |
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