CN104472175A - Method for improving cadmium-contaminated orchard soil remediation capability of solanum photeinocarpum - Google Patents

Method for improving cadmium-contaminated orchard soil remediation capability of solanum photeinocarpum Download PDF

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CN104472175A
CN104472175A CN201410720445.7A CN201410720445A CN104472175A CN 104472175 A CN104472175 A CN 104472175A CN 201410720445 A CN201410720445 A CN 201410720445A CN 104472175 A CN104472175 A CN 104472175A
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photeinocarpum
abscisic acid
orchard
cadmium
soil
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CN104472175B (en
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廖明安
林立金
罗丽
任雅君
汪志辉
吕秀兰
邓群仙
汤福义
杨代宇
张潇
刘春阳
何静
马倩倩
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Sichuan Agricultural University
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00Cultivation of specific crops or plants not otherwise provided for
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00Botany in general
    • A01G7/06Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants

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  • Biodiversity & Conservation Biology (AREA)
  • Ecology (AREA)
  • Forests & Forestry (AREA)
  • Botany (AREA)
  • Environmental Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Cultivation Of Plants (AREA)

Abstract

The invention discloses a method for improving cadmium-contaminated orchard soil remediation capability of solanum photeinocarpum and belongs to the field of remediation of heavy metal pollution environment. The method includes the following steps: planting mining ecotype solanum photeinocarpum in cadmium-contaminated orchard soil by means of direct transplantation or direct seed broadcasting, and after the mining ecotype solanum photeinocarpum enters a rapid growth stage, spraying 1-20umol/L abscisic acid solution on leaf surfaces; at least 30 days after spraying, harvesting overground parts of the mining ecotype solanum photeinocarpum. The method for improving cadmium-contaminated orchard remediation of hyperaccumulator is low in cost, high in operability, environment friendly and effective.

Description

Improve the method that S. photeinocarpum repairs orchard cadmium pollution soil ability
Technical field
The invention belongs to the reparation field of Heavy-Metal-Contaminated Environments, particularly relate to the method for repairing orchard cadmium pollution soil.
Background technology
Along with the factors such as industrial pollution in recent years, field irrigation, material used for agriculture cause cadmium pollution problem to be on the rise.China's cadmium pollution soil area has reached 200,000 square kilometres, accounts for 1/6 of total area under cultivation.As a kind of restorative procedure of emerging and environmental protection, phytoremediation is risen gradually, phytoremediation is exactly utilize specified plant to the accumulation ability of the super common plant far away of specific heavy metal, and by heavy metal, soil enrichment is in plant again, and heavy metal can depart from by plant harvesting from soil the most at last.
S. photeinocarpum (Solanum photeinocarpum) is annual plant of Solanaceae, Gao Keda 1 meter, and be a kind of common orchard weed, known have stronger accumulation ability to cadmium.
But regrettably, it is a kind of potential Cd-hyperaccumulator, because its transhipment coefficient from 1 also distant (evaluation super enriching plant important indicator, namely the cadmium accumulation ability of acrial part exceedes under ground portion, because harvesting is all only gather in acrial part usually, if also gather under ground portion, although cadmium can be removed the most fully simultaneously, but because workload greatly increases, be difficult to possess reality and repair meaning).
And under high concentration cadmium pollution condition, the accumulation ability of S. photeinocarpum to cadmium also declines further.
Therefore how improving S. photeinocarpum to orchard cadmium soil pollution repair ability is that prior art is thirsted for solving but open question for a long time.
Summary of the invention
The object of example of the present invention is to provide and improves the method that S. photeinocarpum repairs orchard cadmium pollution soil ability, is intended to improve the ability that S. photeinocarpum repairs orchard cadmium pollution soil further.
In order to realize foregoing invention object, the technical solution used in the present invention is as follows:
Improve the method that S. photeinocarpum repairs orchard cadmium pollution soil ability, comprise the following steps:
Directly transplanting or seed is adopted directly to broadcast sowing mode by Mine ecology type S. photeinocarpum kind in cadmium pollution orchard soil, after Mine ecology type S. photeinocarpum enters fast growing period, the abscisic acid solution of foliage-spray 1 ~ 20umol/L; After spraying at least 30 days, the acrial part of Mine ecology type S. photeinocarpum is gathered in.
As selection, described abscisic acid solution concentration is 20umol/L.
As further selection, adopt the method for direct transplanting method preparation plant seedlings as follows:
Under natural conditions, by the Mine ecology type S. photeinocarpum seedling directly transplanting collected in cadmium pollution orchard soil, control density is 15 × 15cm.
Select further as another, the method that seed sowing mode prepares seedling is as follows:
Under field conditions (factors), by the Mine ecology type S. photeinocarpum planting seed collected in cadmium pollution orchard soil, seedling grows rear even seedling, and density domination is at 15 × 15cm.
In such scheme, described Mine ecology type S. photeinocarpum refers to source or is grown on the S. photeinocarpum in mining area.Described fast growing period is usually in plantation after one month, and the planting type of S. photeinocarpum is generally: water and guarantee that field soil moisture water-holding capacity is 80%, carry out the daily management of Mine ecology type S. photeinocarpum; Described daily management comprises even seedling, weeds a garden.
Abscisic acid (abscisic acid, ABA), a kind of plant hormone of Developing restraint, gains the name because impelling leaves.Its Main Function has: promote to come off, Developing restraint, promotion dormancy, cause stomatal closure, regulate the growth of seed embryo, increase resistance and impact property and break up.Wherein, as the use increasing resistance, mainly strengthen the freeze proof ability of plant cold resistance, and improve drought resistance and the Salt-endurance of plant.
The mechanism of action: the physiological action of abscisic acid mainly causes dormancy and promotion to come off.With the sprig that abscisic acid process plant growth is vigorous, the state identical with dormancy can be caused; The leaf producing perula shape replaces the trophophyll launched; Reduce the mitotic event of apical meristem; And leaves below and the releasing preventing dormancy can be caused.With the seed that abscisic acid process can be sprouted, dormancy can be made it.This inhibitory action to sprouting can be offset with gibberellin or basic element of cell division process or reverse.The effect that replacement long-day of abscisic acid energy antagonism gibberellin causes long day plant bolting to be bloomed.It can also make minority short day plant bloom under the condition in non-induced cycle.Otherwise several effects of abscisic acid also can be offset with gibberellin.Such as use gibberellin just can overcome abscisic acid to the elongation of inheritance height stalk corn and the inhibitory action to seed germination and potato sprouting.In addition, the effect of abscisic acid is also contrary with the basic element of cell division, and the effect of abscisic acid existing antagonism gibberellin in plant corpus, also has the effect of antagonize cellular mitogen.But these antagonisms are very complicated.Such as Germination of Lettuce Seeds needs light, and gibberellin can replace light.And the effect that the promotion that abscisic acid can offset gibberellin is sprouted, but continue to improve the effect that the concentration of gibberellin can not overcome abscisic acid, the promotion recovering sprouting.
Wherein, because abscisic acid is for the raising of Salt-endurance, mainly because abscisic acid can by the signal transduction on guard cell's film, impel stomatal closure, and the synthesis of induced resistance protein, the expression of portion gene can be regulated and controled simultaneously, strengthen the ability of plant resistant adverse circumstance with this.Therefore, in saline land, abscisic acid is sprayed to common plant and can suppress the absorption of sodium at high concentration ion: suppress too much sodium ion to enter in plant corpus on the one hand, suppress the Competitive assays of other soil nutrient elements of sodium at high concentration ion pair on the other hand, other nutritive elements can smoothly be absorbed by ground cover plant.Final result improves the biomass of plant in saline land.
And heavy metal ion is to the toxic action of plant, also similar saline land, because be all the metal ion of higher concentration: being the murder by poisoning that heavy metal ion enters to plant after in plant corpus on the one hand, is also the Competitive assays of heavy metal ion to other soil nutrient elements on the other hand.
And for heavy metal accumulation plant, but require this plant can as much as possible by heavy metal accumulation in body, obviously from known knowledge, abscisic acid can suppress this process.
Therefore, although common in agricultural production in prior art, by spraying abscisic acid to improve the resistance of the high salt concentration of common plant, but foregoing reason, never someone want to spray abscisic acid by heavy metal enriching plant, to improve the accumulation ability of its heavy metal ion, particularly improve its transhipment coefficient, obviously this deviates from mutually with existing knowledge.
Beneficial effect of the present invention: the present inventor is surprised to find that under study for action, the abscisic acid (1 ~ 20umol/L) spraying certain concentration to the particular type (Mine ecology type) of specific plant (S. photeinocarpum) specifically can improve its accumulation ability to specific heavy metal ion (cadmium), special more expectation other places improves its movement system, makes the S. photeinocarpum originally without remarkable cadmium pollution reparation meaning possess real practical value.
Embodiment
In order to make object of the present invention, technical scheme and advantage clearly understand, below in conjunction with embodiment, the present invention is further elaborated.Should be appreciated that specific embodiment described herein only in order to explain the present invention, be not intended to limit the present invention.
Improve the method that S. photeinocarpum repairs orchard cadmium pollution soil ability, comprise the following steps:
Adopt and directly to transplant or seed directly broadcasts sowing mode by Mine ecology type S. photeinocarpum kind in cadmium pollution orchard soil, after Mine ecology type S. photeinocarpum enters fast growing period, the abscisic acid solution (preferred abscisic acid solution concentration is 20umol/L) of foliage-spray 1 ~ 20umol/L; After spraying at least 30 days, the acrial part of Mine ecology type S. photeinocarpum is gathered in.
Wherein, adopt the method for direct transplanting method preparation plant seedlings as follows:
Under natural conditions, by the Mine ecology type S. photeinocarpum seedling directly transplanting collected in cadmium pollution orchard soil, control density is 15 × 15cm.
Wherein, to prepare the method for seedling as follows for seed sowing mode:
Under field conditions (factors), by the Mine ecology type S. photeinocarpum planting seed collected in cadmium pollution orchard soil, seedling grows rear even seedling, and density domination is at 15 × 15cm.
For convenience of explanation, illustrate only part related to the present invention.
Example 1:(Mine ecology type S. photeinocarpum is tested)
The enforcement place of the embodiment of the present invention: be located at farm, school district, Sichuan Agricultural University Yaan.
Material: Mine ecology type S. photeinocarpum, its seed takes from Hanyuan County, sichuan Province county Tangjiashan Pb-Zn deposits.Man of Tang of Hanyuan County, sichuan Province county Pb-Zn ore district (east longitude: 102 ° 38 ', north latitude: 29 ° 24 ') is positioned at Ya'an, Sichuan Province Hanyuan County Tang local, mean sea level 890m.Now possessing Pb-Zn deposits reserves is 145.8 ten thousand t, and year mining 100,000 t, have exploited 15 years continuously, sporadicly piled up about more than 60 ten thousand m 3abandoned mine slag.Mining area is located in the subtropical climate district, mountain region between north temperate zone and monsoon zone, have typical dry-hot valley climatic characteristic, for many years mean temperature of air 17.9 DEG C, Multi-year average precipitation is 741.8mm, for many years average 1475.8 hours sunshine, average evaporation capacity is 1248.2mm for many years.
The embodiment of the present invention adopts the method for potted plant simulated soil heavy metal pollution, after air-dry for the soil taking from orchard, crushing, crossing 5mm sieve, takes 4.0kg respectively and is loaded in the plastic basin of 18cm × 21cm (height × diameter), add and analyze pure CdCl 22.5H 2o solution, makes its cadmium concentration be 10mg/kg, and fully mixes with soil, naturally places balance mixing for standby use again after 4 weeks.By Mine ecology type S. photeinocarpum seed broadcasting in farmland, grow after 2 true leaves until Mine ecology type S. photeinocarpum and transplant in basin, 2 strains planted by every basin.Enter after fast growing period (planting month) until Mine ecology type S. photeinocarpum, foliage-spray abscisic acid solution.Test arranges 6 concentration process, is respectively: 0,1,5,10,20,40umol/L, each process repeats 3 times, and amount to 18 basins, every basin sprays 50mL abscisic acid solution.Mine ecology type S. photeinocarpum is all positioned in transparent rain shade whole vegetative period, irregularly water according to soil moisture actual conditions and guarantee that soil moisture remains on about 80% of field capacity, spray abscisic acid after one month Mine ecology type S. photeinocarpum be in full-bloom stage, whole strain results.
The invention process result is as follows:
Abscisic acid on the impact of Mine ecology type S. photeinocarpum Cd accumulation amount in table 1:
Table 1 abscisic acid is on the impact of Mine ecology type S. photeinocarpum Cd accumulation amount
With the increase of abscisic acid concentration, Mine ecology type S. photeinocarpum root system Cd accumulation amount, also along with rising (table 1), when to be up to abscisic acid concentration be 40umol/L, compares according to exceeding 97.93%.With the increase of abscisic acid concentration, Mine ecology type S. photeinocarpum cane, blade and acrial part Cd accumulation amount in first increasing the trend of falling afterwards, all with abscisic acid concentration for the highest during 20umol/L, improve 60.60%, 68.36% and 63.94% than contrast respectively.From whole strain Cd accumulation amount, the abscisic acid spraying variable concentrations all improves the Cd accumulation amount of Mine ecology type S. photeinocarpum, maximum when abscisic acid concentration is 20umol/L, is secondly 40umol/L, improves 67.50% and 66.20% respectively than contrast.
Abscisic acid affects in table 2 Mine ecology type S. photeinocarpum cadmium content:
Table 2 abscisic acid affects Mine ecology type S. photeinocarpum cadmium content
Note: transhipment coefficient=acrial part cadmium content/root system cadmium content.
Can be obtained by table 2, when abscisic acid concentration is between 0umol/L-20umol/L, Mine ecology type S. photeinocarpum root system, cane, blade and overground part cadmium content all increase with the increase of abscisic acid concentration; When abscisic acid concentration reaches 40umol/L, decrease when comparatively abscisic acid concentration is 20umol/L, but still be significantly higher than contrast.After the abscisic acid of foliage-spray variable concentrations, Mine ecology type S. photeinocarpum root system cadmium content difference is little; The cadmium content of cane and blade all with abscisic acid concentration for the highest during 20umol/L, improve 34.39%, 32.46% than contrast respectively.The size order of the Mine ecology type S. photeinocarpum acrial part cadmium content of different abscisic acid concentration process is: 20umol/L > 10umol/L > 40umol/L > 5umol/L > 1umol/L > 0umol/L.From transhipment coefficient, still with abscisic acid concentration for the highest during 20umol/L.
Example conclusion: in summary, when abscisic acid concentration is 20umol/L, the cadmium accumulation ability of Mine ecology type S. photeinocarpum is increased dramatically, and particularly transports coefficient more notable lifting, and to the utmost is the standard of super enriching plant close to 1.
Comparison example 1:(agricultural ecological type S. photeinocarpum is tested)
The enforcement place of the embodiment of the present invention: be located at farm, school district, Sichuan Agricultural University Yaan.
Material: agricultural ecological type S. photeinocarpum, its seed takes from farm, school district, Sichuan Agricultural University Yaan.Farm, Sichuan Agricultural University new district (north latitude 30 ° 23 ', east longitude 103 ° 48 '), be positioned at Sichuan Province's YAAN RAIN CITY DISTRICT, mean sea level 620m, belong to moistening monsoon climatic region, subtropics, for many years mean temperature of air 16.2 DEG C, Multi-year average precipitation is 1743.3mm, for many years average 1035 hours sunshine, average evaporation capacity is 1011.2mm for many years.
The embodiment of the present invention adopts the method for potted plant simulated soil heavy metal pollution, after air-dry for the soil taking from orchard, crushing, crossing 5mm sieve, takes 4.0kg respectively and is loaded in the plastic basin of 18cm × 21cm (height × diameter), add and analyze pure CdCl 22.5H 2o solution, makes its cadmium concentration be 10mg/kg, and fully mixes with soil, naturally places balance mixing for standby use again after 4 weeks.By agricultural ecological type S. photeinocarpum seed broadcasting in farmland, grow after 2 true leaves until agricultural ecological type S. photeinocarpum and transplant in basin, 2 strains planted by every basin.Enter after fast growing period (planting month) until agricultural ecological type S. photeinocarpum, foliage-spray abscisic acid solution.Test arranges 6 concentration process, is respectively: 0,1,5,10,20,40umol/L, each process repeats 3 times, and amount to 18 basins, every basin sprays 50mL abscisic acid solution.Agricultural ecological type S. photeinocarpum is all positioned in transparent rain shade whole vegetative period, irregularly water according to soil moisture actual conditions and guarantee that soil moisture remains on about 80% of field capacity, spray abscisic acid after one month agricultural ecological type S. photeinocarpum be in full-bloom stage, whole strain results.
The invention process result is as follows:
Abscisic acid on the impact of agricultural ecological type S. photeinocarpum Cd accumulation in table 3.
Table 3 abscisic acid is on the impact of agricultural ecological type S. photeinocarpum Cd accumulation
With the increase of abscisic acid concentration, agricultural ecological type S. photeinocarpum root system Cd accumulation amount is in the trend increased, but cane, blade, acrial part and whole strain Cd accumulation amount are in first increasing the trend (table 1) of falling afterwards.Agricultural ecological type S. photeinocarpum cane and blade Cd accumulation amount all reach maximum when abscisic acid concentration is 20umol/L, improve 34.02%, 49.85% respectively than contrasting separately; And root system Cd accumulation is maximum when abscisic acid concentration is 40umol/L, improve 44.35% than contrast, but be only the Cd accumulation amount of 20umol/L a little more than abscisic acid concentration.With regard to overground part Cd accumulation amount, when abscisic acid concentration is 20umol/L, Cd accumulation amount is maximum, and comparatively contrast improves 42.60%.The size order of agricultural ecological type S. photeinocarpum whole strain Cd accumulation amount is: 20umol/L > 40umol/L > 10umol/L > 5umol/L > 1umol/L > 0umol/L.
Abscisic acid affects in table 4 agricultural ecological type S. photeinocarpum cadmium content:
Table 4 abscisic acid affects agricultural ecological type S. photeinocarpum cadmium content
Note: transhipment coefficient=acrial part cadmium content/root system cadmium content.
As shown in Table 4, when abscisic acid concentration is between 0umol/L-20umol/L, agricultural ecological type S. photeinocarpum root system, cane, blade and overground part cadmium content all increase with the increase of abscisic acid concentration, reaching maximum when abscisic acid concentration reaches 20umol/L, improve 23.20%, 17.82%, 30.13%, 24.71% than contrasting separately respectively; When concentration reaches 40umol/L, each several part cadmium content comparatively abscisic acid concentration is that 20umol/L decreases, but is all significantly higher than contrast.Secondly with regard to regard to transhipment coefficient, with abscisic acid concentration for maximum during 10umol/L, for abscisic acid concentration is 20umol/L, minimum for abscisic acid concentration be 40umol/L process.
Example conclusion: in summary, when abscisic acid concentration is 20umol/L, the cadmium accumulation ability of agricultural ecological type S. photeinocarpum is the highest, and transports coefficient and also promote to some extent.But to show obvious inconsistent compared with Mine ecology type S. photeinocarpum: the cadmium accumulation ability under this abscisic acid concentration is lower than Mine ecology type S. photeinocarpum, maximum difference is, the transhipment coefficient of abscisic acid to agricultural ecological type S. photeinocarpum does not unexpectedly have essence and promotes, the highest at 40umol/L, and when 20umol/L almost without lifting.
Comparison example 2: abscisic acid is on S. photeinocarpum biomass impact (processing mode is as front):
Abscisic acid affects in table 5 agricultural ecological type S. photeinocarpum biomass.
Table 5 abscisic acid affects agricultural ecological type S. photeinocarpum biomass
With the rising of abscisic acid concentration, agricultural ecological type S. photeinocarpum root biomass, cane biomass, Leaf biomass, the upperground part biomass and total biomass all significantly increase (table 5) with the rising of abscisic acid concentration.When abscisic acid concentration is 40umol/L, agricultural ecological type S. photeinocarpum root biomass, the upperground part biomass and whole strain biomass all reach maximum, add 18.62%, 16.96%, 17.33% respectively than contrasting separately.With regard to agricultural ecological type S. photeinocarpum root system and cane biomass, when abscisic acid concentration is slow more than increasing trend during 20umol/L.From agricultural ecological type S. photeinocarpum root/shoot ratio, between each process, difference is little, but all higher than contrast.
Abscisic acid the results are shown in Table 6 to the impact of Mine ecology type S. photeinocarpum biomass:
Table 6 abscisic acid affects Mine ecology type S. photeinocarpum biomass
As shown in Table 6, along with the increase of abscisic acid concentration, Mine ecology type S. photeinocarpum root biomass significantly increases, and when abscisic acid concentration reaches 40umol/L, root biomass adds 82.05% than contrast.Mine ecology type S. photeinocarpum cane, blade, Aboveground Biomass of Young and whole strain biomass are identical with root system variation tendency, and this is consistent with the performance of agricultural ecological type S. photeinocarpum.Single with regard to cane biomass, during abscisic acid low concentration time (1umol/L) with contrast difference not quite, biomass is still maximum when abscisic acid concentration reaches 40umol/L, comparatively contrasts and adds 31.07%.With regard to Leaf biomass, still when abscisic acid low concentration (1umol/L), change is not remarkable, and increases with the increase of abscisic acid concentration.The whole strain biomass of Mine ecology type S. photeinocarpum is that 1umol/L, 5umol/L, 10umol/L, 20umol/L, 40umol/L add 4.15%, 14.92%, 23.98%, 29.49%, 42.20% than contrast respectively in abscisic acid concentration.
Example conclusion: abscisic acid on the impact of S. photeinocarpum biomass and abscisic acid basically identical to common plant, no matter be agricultural ecological type or Mine ecology type, along with the raising of concentration, biomass substantially linear improve.But its performance is but unexpectedly completely different with the impact of transporting coefficient on the cadmium accumulation ability of the S. photeinocarpum of different ecological type with abscisic acid.
It can thus be appreciated that, although abscisic acid improves the cadmium accumulation ability of S. photeinocarpum and the agnogenio of transhipment coefficient, but identifiable, the biomass brought with abscisic acid promotes irrelevant, and namely the characteristic that can improve salt resistance of known abscisic acid to common plant there is no instruction meaning.
The foregoing is only preferred embodiment of the present invention, not in order to limit the present invention, all any amendments done within the spirit and principles in the present invention, equivalent replacement and improvement etc., all should be included within protection scope of the present invention.

Claims (4)

1. improve the method that S. photeinocarpum repairs orchard cadmium pollution soil ability, it is characterized in that comprising the following steps:
Directly transplanting or seed is adopted directly to broadcast sowing mode by Mine ecology type S. photeinocarpum kind in cadmium pollution orchard soil, after Mine ecology type S. photeinocarpum enters fast growing period, the abscisic acid solution of foliage-spray 1 ~ 20umol/L; After spraying at least 30 days, the acrial part of Mine ecology type S. photeinocarpum is gathered in.
2. a kind of method improving S. photeinocarpum reparation orchard cadmium pollution soil ability as claimed in claim 1, it is characterized in that, described abscisic acid solution concentration is 20umol/L.
3. a kind of method improving S. photeinocarpum reparation orchard cadmium pollution soil ability as claimed in claim 1 or 2, is characterized in that, adopts the method for direct transplanting method preparation plant seedlings as follows:
Under natural conditions, by the Mine ecology type S. photeinocarpum seedling directly transplanting collected in cadmium pollution orchard soil, control density is 15 × 15cm.
4. a kind of method improving S. photeinocarpum reparation orchard cadmium pollution soil ability as claimed in claim 1 or 2, it is characterized in that, the method that seed sowing mode prepares seedling is as follows:
Under field conditions (factors), by the Mine ecology type S. photeinocarpum planting seed collected in cadmium pollution orchard soil, seedling grows rear even seedling, and density domination is at 15 × 15cm.
CN201410720445.7A 2014-12-01 2014-12-01 Improve the method that S. photeinocarpum repairs orchard cadmium pollution soil ability Expired - Fee Related CN104472175B (en)

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CN107787754A (en) * 2017-11-10 2018-03-13 四川农业大学 A kind of method for promoting tree tomato to grow and reduce its aerial part cadmium content
CN108704928A (en) * 2018-05-21 2018-10-26 中南民族大学 It is a kind of to improve composite drug and its preparation method and application of the plant to Metal uptake turn-over capacity
CN114029338A (en) * 2021-11-08 2022-02-11 上海交通大学 Optimized restoration method for black nightshade in moderate cadmium polluted soil

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