CN104450666A - Biological mariculture substrate improver and preparation method thereof - Google Patents
Biological mariculture substrate improver and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a biological mariculture substrate improver and a preparation method thereof, for solving the problem that a mariculture substrate cannot be thoroughly purified. The preparation method comprises the following steps: 1) acclimatizing bacillus in a normal state by using a culture medium prepared from sea water, thereby enabling the bacillus to grow and reproduce in sea water and obtaining a substrate modification engineering bacterial liquid; 2) uniformly mixing zeolite powder, medical stone powder and montmorillonite, and performing superfine grinding on the mixture, thereby obtaining immobilized carrier micro powder of 500-1000 meshes; 3) mixing the immobilized carrier micro powder with the substrate modification engineering bacterial liquid, sufficiently adsorbing, and drying, thereby preparing granules A; 4) mixing magnesium silicate nano powder with a liquid adhesive, thereby preparing a nano colloid coating agent; and 5) uniformly wrapping the nano colloid coating agent on the surfaces of the granules A, and pelleting and molding by using pelleting equipment, thereby obtaining granules B, that is, the biological mariculture substrate improver. The biological mariculture substrate improver disclosed by the invention can be used for thoroughly purifying mariculture substrates.
Description
Technical field
The present invention relates to technical field of aquaculture, particularly a kind of sea farming substrate biological modification agent and preparation method thereof.
Background technology
In recent years, along with culture fishery is flourish, breed variety is aging and degenerate, and aquatic animal disease frequently occurs, and aquaculture water environment goes from bad to worse, and this three large problem has become the bottleneck hindering China's culture fishery development.Especially the aquaculture model based on bait throwing in, the eutrophy factors such as the residual body of residual bait, ight soil, dead organisms, the settling of cultivating pool substrate, nitrogen (N), phosphorus (P) enter water body, chemical oxygen demand (COD) (COD) in aquaculture water, biological oxygen demand (BOD) severe overweight, water body is gone from bad to worse, and aquatic animal disease frequently occurs.
According to statistics, the nitrogen in artificial bait throwing in input shrimp pond accounts for about 90% of total input, 19% is wherein only had to be converted into nitrogen in shrimp body, remaining nitrogen of 62% ~ 68% accumulates in the bottom sludge of shrimp pond, in addition have 8% ~ 12% to be present in Chi Shuizhong with forms such as suspended particle nitrogen dissolved organic nitrogen, dissolved Inorganic Nitrogen, cause the severe exacerbation of pond water quality.
" breed fish and first support water, modify-water first changes the end ", this is aquaculture industry " jargon ", and in visible sea farming, the difference of water surface for aquaculture substrate directly affects the benefit of aquaculture.Even polluted seawater, welding.Therefore improve and optimize aquaculture ecotope, promoting that the bacteriostatic and disease prevention functional meaning of aquaculture organisms growth and increase aquaculture organisms is great.
The Chinese invention patent that Authorization Notice No. is " CN100484395C " discloses a kind of culture fishery preparation and preparation method thereof, and the components by weight of described preparation is: zeolite 70 ~ 80kg, medical stone 0.5 ~ 2kg, CuSO
40.5 ~ 2kg, CaO
20.5 ~ 2kg, preparation method is by zeolite, medical stone, CuSO according to the weight ratio of above-mentioned formulation component
4, CaO
2add stirrer respectively, be pulverized and mixed through mechanical stirring and form, described preparation have absorption aquaculture water in objectionable impurities as ammonia nitrogen, hydrogen sulfide; Increase dissolved oxygen content; Sterilization; Increase the comprehensive action such as micronutrient levels, significantly improve breeding ecological environment, but to improve effect not remarkable for said preparation.
Summary of the invention
The object of the present invention is to provide a kind of sea farming substrate biological modification agent and preparation method thereof, to solve the problem of thorough purifying sea water cultivation substrate.
Technical scheme of the present invention is achieved in that
A preparation method for sea farming substrate biological modification agent, comprises the following steps:
1) the substratum domestication made through seawater by the genus bacillus under normality, purification, enable described genus bacillus growth and breeding in the seawater, obtain and change engineering bacteria bacterium liquid on earth;
2) according to weight parts, by 30 ~ 100 parts of zeolite powders, 3 ~ 15 parts of medical stone powders, 8 ~ 20 parts of montmorillonite Homogeneous phase mixing, then this mixture is carried out micronizing, obtain 500 order ~ 1000 object fixation support micro mists;
3) engineering bacteria bacteria liquid is changed by described fixation support micro mist and the described end long-pending than mixing for 1:1 ~ 5:1, fully after absorption, dry, granulate, obtain particle A;
4) be that 1:1 ~ 5:1 mixes by Magnesium Silicate q-agent nano powder and liquid adhesive weight ratio, prepare nanometer colloid coating agent;
5) described nanometer colloid coating agent is coated on described particle A surface, described nanometer colloid coating agent and described particle A weight ratio are 5:3 ~ 9:3, through facility for granulating pelletizing forming, obtain particle B, i.e. sea farming substrate biological modification agent.
Wherein, step 2) in, according to weight parts, by 30 ~ 100 parts of zeolite powders, 3 ~ 15 parts of medical stone powders, 8 ~ 20 parts of montmorillonite Homogeneous phase mixing.Zeolite has the function such as ion-exchange, absorption, can adsorb objectionable impurities in aquaculture water preferably as ammonia nitrogen, hydrogen sulfide etc.Wheat stone flour has biological activity, adsorptivity, mineralising, water quality survey function; The adsorptivity of wheat stone flour is embodied in can to important meals ion Pb, Hg, Cr, Cd, the As in water, and the hazardous and noxious substances such as organic matter, germ have very strong adsorption; The mineralising sex expression of wheat stone flour is for be immersed in water by medical stone powder, and water will be mineralized into mineralized water; Water quality survey sex expression is the effect that medical stone has two-ways regulation PH, acidity or alkaline water can be made to regulate in neutral, water quality is refined, sensitization.And montmorillonite has cleaning action and adsorption.By the mixture micronizing of three, obtaining granular size is 500 order ~ 1000 object fixation support micro mists, specific surface area is very big, greatly improve the purification function of described fixation support micro mist, described fixation support micro mist can in active adsorption aquaculture water objectionable impurities as ammonia nitrogen, hydrogen sulfide, and there is sterilization, increase dissolved oxygen content, increase the effects such as micronutrient levels, can obviously improve and Optimal culture ecotope.
It is adopt genus bacillus to tame in the substratum prepared by seawater to cultivate gained that the end described in step 3) changes engineering bacteria bacterium liquid.Genus bacillus has strong decomposition carbon system, nitrogen system, phosphorus system, sulphur system pollutent, and the ability of decomposing protein, complicated polysaccharide, water soluble organic substance can well purifying sea water environment.Genus bacillus also produces useful thalline while N element, S element and plurality of heavy metal element absorption, eats for hydrobiont and Aquatic farming animals.In addition, in step 3), particle A can be prepared in the following manner: the end changes after engineering bacteria bacterium liquid and zeolite powder fully adsorb, counter current drying, after drying powdered ingredients, described powdered ingredients pelletizing forming in bowling machine.Preferably, the diameter of described particle A is 1-5mm.
Be mixed and made into nanometer colloid coating agent (polymer binder coating agent) by Magnesium Silicate q-agent nano powder and described nanometer colloidal particle described in step 4), be used for bag by described particle A, the shelf time of microbial preparation can be extended on the one hand; On the other hand, Magnesium Silicate q-agent nano powder is feed grade photochemical catalysis Magnesium Silicate q-agent, and intermolecular space can be made to increase, and has the function increasing dissolved oxygen.Photochemical catalysis can make capsulating material reach effect without harmful bacteria, can also increase product uniformity coefficient.Photochemical catalysis is all effective to the huge number organism from alkyl to carboxylic acid, nine large classes, 114 kinds of pollutents that Environmental Protection Agency announces all are proved and are governed by photochemical catalysis, even if to atom organism as halohydrocarbon, dyestuff, itrogenous organic substance, organic phosphorous insecticide also have good removal effect, generally can reach through sustained reaction and purify completely.Mainly use removing miscellaneous bacteria (10000W UV-light) function of light-catalysed encrusting substance itself, oxygen-supplying function in the product and extended the product stock time.
Preferably, the described end changes the concentration of engineering bacteria bacterium liquid is 1*10
7~ 5*10
9cfu/ml.In this concentration range, the described end, changes engineering bacteria bacterium liquid and just effectively can decompose carbon system in cultivation substrate, nitrogen system, phosphorus system, sulphur system pollutent, and can effectively decomposing protein, complicated polysaccharide, water soluble organic substance.
Preferably, described genus bacillus is subtilis, slowly genus bacillus and Bacillus licheniformis.These three kinds of genus bacillus of experimental verification to breeding seawater and breeding seawater substrate decontamination effect improving very good.
Preferably, bacillus living under described normality is cultivated through defined medium, these substratum details are: after wheat bran 50.0-80.0g and soybean cake powder 30.0-50.0g mixes, add 0.2-0.3%NaOH solution 1.0-1.5L, boil 1h, neutralize pH7 with HCl, then add agar 20.0-30.0g, after fusing, packing sterilizing.
Preferably, step 1) described in agar-agar soln or the full sea water of the substratum that makes of seawater to be seawater weight content be 10-99%, the genus bacillus under described normality is carried out domestication cultivation successively in seawater content sea water medium from low to high.
Preferably, step 2) in, according to weight parts, by 80 parts of zeolite powders, 8 parts of medical stone powders, 12 parts of montmorillonite Homogeneous phase mixing, then this mixture is carried out micronizing, obtain 500 object fixation support micro mists.
As preferably, the liquid adhesive described in step 4) is mixed according to weight ratio 2:1 ~ 8:1 with xanthan gum by sodium polyacrylate.
As preferably, in the nano powder of Magnesium Silicate q-agent described in step 4), mix titanic oxide nano, described titanic oxide nano and described Magnesium Silicate q-agent nano powder weight ratio 1:12 ~ 1:8; Step 5) in, described nanometer colloid coating agent adopts spray pattern to be evenly coated on described particle A surface.Described titanium dioxide is feed grade titanium dioxide, in addition, titanium dioxide is insoluble compound, studies several five kinds of comprising people, both do not had a large amount of absorptions not have organized deposition after display picked-up titanium dioxide, absorbing a small amount of titanium ion does not have toxic effect yet.Magnesium Silicate q-agent, pharmaceutically as relieving haperacidity medicine, with hydrochloric acid in gastric juice and protection ulcer surface in energy, also can cook deodorizing composition, discoloring agent and food grade anti-caking agent, flocculating aids, fruit glaze agent.Seawater is weakly alkaline, when product is slightly acidic can slowly in and weak acid, its reaction principle is as follows: 2MgO3SiO
2xH
2o+4HCl=2MgCl
2+ 3SiO
2+ (X+2) H
2o.Titanium dioxide, silicon-dioxide are again good photocatalyst materials, in substrate improved, process, under low temperature, sunlight, thoroughly can purify substrate.Step 5) in, described nanometer colloid coating agent can adopt spray pattern to be coated on described particle A surface.Spray pattern is coated, evenly coated, simple and feasible.
Preferably, described nanometer colloid coating agent and described particle A weight ratio are 7:3.
Utilize the sea farming substrate biological modification agent that the preparation method of described sea farming substrate biological modification agent obtains, clad nano colloid coating agent outside described particle A.
Further, the sea farming substrate biological modification agent that the inventive method obtains, in ball-type, diameter is 1.5-7mm.Further, the even coatedparticles A of described nanometer colloid coating agent.
Beneficial effect of the present invention is:
The sea farming substrate biological modification agent that the inventive method is obtained, directly with microbial project fermentation genus bacillus and photochemical catalysis Magnesium Silicate q-agent nanometre glue powder particles purifying sea water and bottom environment, noresidue, without rebounding; Utilize natural zeolite to carry genus bacillus uniform fold seawater substrate top layer, fixation support micro mist energy adsorbing contaminant, genus bacillus utilizes its microorganism exceptional function removal of contamination, thus thorough purifying sea water substrate.The shelf time of microbial preparation can be extended with nanometer colloid coating agent, intermolecular space can also be made to increase, there is the function increasing dissolved oxygen.In addition, the agent of sea farming substrate biological modification does not use any chemical feedstocks and microbiotic, fully use biotechnology and microbial engineering makes ecological, green, efficiently, environmental protection, safe marine organisms additive product.
Accompanying drawing explanation
Fig. 1 is sea farming substrate biological modification agent preparation technology schema of the present invention;
Fig. 2 is the time dependent comparison diagram of breeding seawater pH value in control group and experimental group;
Fig. 3 is the time dependent comparison diagram of breeding seawater dissolved oxygen DO in control group and experimental group;
Fig. 4 is the time dependent comparison diagram of chemical oxygen demand COD of breeding seawater in control group and experimental group;
Fig. 5 is bed mud organic rising situation comparison diagram in control group and experimental group.
Embodiment
Be clearly and completely described to the technical scheme in the embodiment of the present invention below, obviously, described embodiment is only a part of embodiment of the present invention, instead of whole embodiments.Based on the embodiment in the present invention, those of ordinary skill in the art, not making the every other embodiment obtained under creative work prerequisite, belong to the scope of protection of the invention.
As shown in Figure 1, the preparation method of a kind of sea farming substrate biological modification agent, comprises the following steps:
1) the substratum domestication made through seawater by the genus bacillus under normality, purification, enable described genus bacillus growth and breeding in the seawater, obtain and change engineering bacteria bacterium liquid on earth;
2) according to weight parts, by 30 ~ 100 parts of zeolite powders, 3 ~ 15 parts of medical stone powders, 8 ~ 20 parts of montmorillonite Homogeneous phase mixing, then this mixture is carried out micronizing, obtain 500 order ~ 1000 object fixation support micro mists;
3) engineering bacteria bacteria liquid is changed by described fixation support micro mist and the described end long-pending than mixing for 1:1 ~ 5:1, fully after absorption, dry, granulate, obtain particle A;
4) be that 1:1 ~ 5:1 mixes by Magnesium Silicate q-agent nano powder and liquid adhesive weight ratio, prepare nanometer colloid coating agent;
5) described nanometer colloid coating agent is coated on described particle A surface, described nanometer colloid coating agent and described particle A weight ratio are 5:3 ~ 9:3, through facility for granulating pelletizing forming, obtain particle B, i.e. sea farming substrate biological modification agent.
Preferably, the end changes the concentration of engineering bacteria bacterium liquid is 1*10
7~ 5*10
9cfu/ml.
Preferably, genus bacillus is subtilis, slowly genus bacillus and Bacillus licheniformis.
Preferably, bacillus living under normality is cultivated through defined medium, these substratum details are: after wheat bran 50.0-80.0g and soybean cake powder 30.0-50.0g mixes, add 0.2-0.3%NaOH solution 1.0-1.5L, boil 1h, neutralize pH7 with HCl, then add agar 20.0-30.0g, after fusing, packing sterilizing.
Preferably, step 1) agar-agar soln or the full sea water of the substratum that makes of seawater to be seawater weight content be 10-99%, the genus bacillus under normality is carried out domestication cultivation successively in seawater content sea water medium from low to high.
Preferably, step 2) in, according to weight parts, by 80 parts of zeolite powders, 8 parts of medical stone powders, 12 parts of montmorillonite Homogeneous phase mixing, then this mixture is carried out micronizing, obtain 500 object fixation support micro mists.
As preferably, the liquid adhesive described in step 4) is mixed according to weight ratio 2:1 ~ 8:1 with xanthan gum by sodium polyacrylate.
Preferably, in the nano powder of Magnesium Silicate q-agent described in step 4), mix titanic oxide nano, described titanic oxide nano and described Magnesium Silicate q-agent nano powder weight ratio 1:12 ~ 1:8; Step 5) in, described nanometer colloid coating agent adopts spray pattern to be evenly coated on described particle A surface.
Preferably, described nanometer colloid coating agent and described particle A weight ratio are 7:3.
Utilize the sea farming substrate biological modification agent that the preparation method of sea farming substrate biological modification of the present invention agent obtains, clad nano colloid coating agent outside component A.
Embodiment 1:
A preparation method for sea farming substrate biological modification agent, comprises the following steps:
1) subtilis is inoculated in defined medium cultivates at 30 DEG C, then the subtilis under the normality obtained is cultivated respectively through domestication in the agar-agar soln of seawater content 10%, 50%, finally cultivate in the seawater of 100%, can growth and breeding in the seawater to genus bacillus.Finally to be able to seawater be the concentration of solute is 1-9*10
7the end of cfu/ml, changes engineering bacteria bacterium liquid;
Wherein, said defined medium details are: wheat bran 50.0-80.0g and soybean cake powder 30.0-50.0g adds 0.2-0.3%NaOH solution 1.0-1.5L, boils 1h, neutralize pH7 with HCl, then add agar 20.0-30.0g after mixing, after fusing, and packing sterilizing.
2) by 60kg zeolite powder, 6kg medical stone powder, 16kg montmorillonite Homogeneous phase mixing, then this mixture is carried out micronizing, obtain 500 object fixation support micro mists;
3) change by fixation support micro mist and the end that engineering bacteria bacteria liquid is long-pending mix than for 1:1, abundant adsorb after, counter current drying, obtains powdered ingredients, by this powdered ingredients pelletizing forming in bowling machine, obtains the particle A that particle diameter is 1-3mm;
4) be that 1:1 mixes by Magnesium Silicate q-agent nano powder and liquid adhesive weight ratio, prepare nanometer colloid coating agent; Wherein, in Magnesium Silicate q-agent nano powder, mix feed grade titanic oxide nano, feed grade titanic oxide nano mixes by 1:12 weight ratio with Magnesium Silicate q-agent nano powder; Liquid-containing binder is mixed by sodium polyacrylate and xanthan gum weight ratio 2:1.
5) nanometer colloid coating agent certain mass step 4) prepared adopts spray pattern to be evenly coated on particle A surface, nanometer colloid coating agent and particle A weight ratio are 5:3, through facility for granulating pelletizing forming, obtain the spherical granules B that particle diameter is 1.5-5mm, i.e. sea farming substrate biological modification agent.The sea farming substrate biological modification agent that the present embodiment is obtained, even clad nano colloid coating agent outside component A.
Embodiment 2:
A preparation method for sea farming substrate biological modification agent, comprises the following steps:
1) slow genus bacillus to be inoculated in defined medium and to cultivate at 30 DEG C, then the slow genus bacillus under the normality obtained is cultivated respectively through domestication in the agar-agar soln of seawater content 10%, 50%, 99%, finally cultivate in the seawater of 100%, can growth and breeding in the seawater to genus bacillus.Finally to be able to seawater be the concentration of solute is 1-5*10
9the end of cfu/ml, changes engineering bacteria bacterium liquid;
Wherein, said defined medium details are: wheat bran 50.0g and soybean cake powder 30.0g adds 0.2%NaOH solution 1.0L, boils 1h, neutralize pH7 with HCl, then add agar 20.0g after mixing, after fusing, and packing sterilizing.
2) by 100kg zeolite powder, 15kg medical stone powder, 20kg montmorillonite Homogeneous phase mixing, then this mixture is carried out micronizing, obtain 1000 object fixation support micro mists;
3) change by fixation support micro mist and the end that engineering bacteria bacteria liquid is long-pending mix than for 5:1, abundant adsorb after, counter current drying, obtains powdered ingredients, by this powdered ingredients pelletizing forming in bowling machine, obtains the particle A that particle diameter is 3-5mm;
4) be that 5:1 mixes by Magnesium Silicate q-agent nano powder and nanometer colloidal particle weight ratio, prepare nanometer colloid coating agent; Wherein, in Magnesium Silicate q-agent nano powder, mix feed grade titanic oxide nano, feed grade titanic oxide nano mixes by 1:8 weight ratio with Magnesium Silicate q-agent nano powder; Liquid-containing binder is mixed by sodium polyacrylate and xanthan gum weight ratio 8:1;
5) nanometer colloid coating agent certain mass step 4) prepared adopts spray pattern to be evenly coated on particle A surface, nanometer colloid coating agent and particle A weight ratio are 9:3, through facility for granulating pelletizing forming, obtain the spherical granules B that particle diameter is 5-7mm, i.e. sea farming substrate biological modification agent.The sea farming substrate biological modification agent that the present embodiment is obtained, even clad nano colloid coating agent outside component A.
Embodiment 3:
A preparation method for sea farming substrate biological modification agent, comprises the following steps:
1) subtilis is inoculated in defined medium cultivates at 30 DEG C, then the subtilis under the normality obtained is cultivated respectively through domestication in the agar-agar soln of seawater content 10%, 50%, finally cultivate in the seawater of 100%, can growth and breeding in the seawater to genus bacillus.Finally to be able to seawater be the concentration of solute is 1-5*10
7the end of cfu/ml, changes engineering bacteria bacterium liquid;
Wherein, said defined medium details are: wheat bran 50.0g and soybean cake powder 30.0g adds 0.2%NaOH solution 1.0L, boils 1h, neutralize pH7 with HCl, then add agar 20.0g after mixing, after fusing, and packing sterilizing.
2) by 80kg zeolite powder, 8kg medical stone powder, 12kg montmorillonite Homogeneous phase mixing, then this mixture is carried out micronizing, obtain 500 object fixation support micro mists;
3) change by fixation support micro mist and the end that engineering bacteria bacteria liquid is long-pending mix than for 3:1, abundant adsorb after, counter current drying, obtains powdered ingredients, by this powdered ingredients pelletizing forming in bowling machine, obtains the particle A that particle diameter is 1-3mm;
4) be that 3:1 mixes by Magnesium Silicate q-agent nano powder and nanometer colloidal particle weight ratio, prepare nanometer colloid coating agent; Wherein, in Magnesium Silicate q-agent nano powder, mix feed grade titanic oxide nano, feed grade titanic oxide nano mixes by 1:10 weight ratio with Magnesium Silicate q-agent nano powder; Liquid-containing binder is mixed by sodium polyacrylate and xanthan gum weight ratio 5:1;
5) nanometer colloid coating agent certain mass step 4) prepared adopts spray pattern to be evenly coated on particle A surface, nanometer colloid coating agent and particle A weight ratio are 7:3, through facility for granulating pelletizing forming, obtain the spherical granules B that particle diameter is 1.5-5mm, i.e. sea farming substrate biological modification agent.The sea farming substrate biological modification agent that the present embodiment is obtained, even clad nano colloid coating agent outside component A.
Embodiment 4:
A preparation method for sea farming substrate biological modification agent, comprises the following steps:
1) Bacillus licheniformis is inoculated in defined medium cultivates at 30 DEG C, then the Bacillus licheniformis under the normality obtained is cultivated respectively through domestication in the agar-agar soln of seawater content 10%, 50%, finally cultivate in the seawater of 100%, can growth and breeding in the seawater to genus bacillus.Finally to be able to seawater be the concentration of solute is 5*10
8~ 1*10
9the end of cfu/ml, changes engineering bacteria bacterium liquid;
Wherein, said defined medium details are: wheat bran 50.0g and soybean cake powder 30.0g adds 0.2%NaOH solution 1.0L, boils 1h, neutralize pH7 with HCl, then add agar 20.0g after mixing, after fusing, and packing sterilizing.
2) according to weight parts, by 100kg zeolite powder, 10kg part medical stone powder, 15kg montmorillonite Homogeneous phase mixing, then this mixture is carried out micronizing, obtain 800 object fixation support micro mists;
3) change by fixation support micro mist and the end that engineering bacteria bacteria liquid is long-pending mix than for 2:1, abundant adsorb after, counter current drying, obtains powdered ingredients, by this powdered ingredients pelletizing forming in bowling machine, obtains the particle A that particle diameter is 1-3mm;
4) be that 2:1 mixes by Magnesium Silicate q-agent nano powder and nanometer colloidal particle weight ratio, prepare nanometer colloid coating agent; Wherein in Magnesium Silicate q-agent nano powder, mix feed grade titanic oxide nano, feed grade titanic oxide nano mixes by 1:11 weight ratio with Magnesium Silicate q-agent nano powder; Liquid-containing binder is mixed by sodium polyacrylate and xanthan gum weight ratio 5:1;
5) nanometer colloid coating agent certain mass step 4) prepared adopts spray pattern to be evenly coated on particle A surface, nanometer colloid coating agent and particle A weight ratio are 8:3, through facility for granulating pelletizing forming, obtain the spherical granules B that particle diameter is 1.5-5mm, i.e. sea farming substrate biological modification agent.The sea farming substrate biological modification agent that the present embodiment is obtained, even clad nano colloid coating agent outside component A.
Embodiment 5
A preparation method for sea farming substrate biological modification agent, comprises the following steps:
1) Bacillus coagulans is inoculated in defined medium cultivates at 30 DEG C, then the Bacillus coagulans under the normality obtained is cultivated respectively through domestication in the agar-agar soln of seawater content 10%, 60%, 90%, finally cultivate in the seawater of 100%, can growth and breeding in the seawater to genus bacillus.Finally to be able to seawater be the concentration of solute is 8*10
8the end of cfu/ml, changes engineering bacteria bacterium liquid;
Wherein, said defined medium details are: wheat bran 80.0g and soybean cake powder 50.0g adds 0.3%NaOH solution 1.5L, boils 1h, neutralize pH7 with HCl, then add agar 30.0g after mixing, after fusing, and packing sterilizing.
2) according to weight parts, by 70kg zeolite powder, 12kg part medical stone powder, 13kg montmorillonite Homogeneous phase mixing, then this mixture is carried out micronizing, obtain 600 object fixation support micro mists;
3) change by fixation support micro mist and the end that engineering bacteria bacteria liquid is long-pending mix than for 4:1, abundant adsorb after, counter current drying, obtains powdered ingredients, by this powdered ingredients pelletizing forming in bowling machine, obtains the particle A that particle diameter is 2-2.5mm;
4) be that 4:1 mixes by Magnesium Silicate q-agent nano powder and nanometer colloidal particle weight ratio, prepare nanometer colloid coating agent; Wherein in Magnesium Silicate q-agent nano powder, mix feed grade titanic oxide nano, feed grade titanic oxide nano mixes by 1:9 weight ratio with Magnesium Silicate q-agent nano powder; Liquid-containing binder is mixed by sodium polyacrylate and xanthan gum weight ratio 3:1;
5) nanometer colloid coating agent certain mass step 4) prepared adopts spray pattern to be evenly coated on particle A surface, nanometer colloid coating agent and particle A weight ratio are 8:3, through facility for granulating pelletizing forming, obtain the spherical granules B that particle diameter is 2-3.5mm, i.e. sea farming substrate biological modification agent.The sea farming substrate biological modification agent that the present embodiment is obtained, even clad nano colloid coating agent outside component A.
Embodiment 6
A preparation method for sea farming substrate biological modification agent, comprises the following steps:
1) Bacillus circulans is inoculated in defined medium cultivates at 30 DEG C, then the Bacillus circulans under the normality obtained is cultivated respectively through domestication in the agar-agar soln of seawater content 10%, 30%, 70%, finally cultivate in the seawater of 100%, can growth and breeding in the seawater to genus bacillus.Finally to be able to seawater be the concentration of solute is 2*10
9the end of cfu/ml, changes engineering bacteria bacterium liquid;
Wherein, said defined medium details are: wheat bran 60.0g and soybean cake powder 40.0g adds 0.2%NaOH solution 1.0L, boils 1h, neutralize pH7 with HCl, then add agar 25.0g after mixing, after fusing, and packing sterilizing.
2) according to weight parts, by 90kg zeolite powder, 8kg part medical stone powder, 10kg montmorillonite Homogeneous phase mixing, then this mixture is carried out micronizing, obtain 900 object fixation support micro mists;
3) change by fixation support micro mist and the end that engineering bacteria bacteria liquid is long-pending mix than for 2:1, abundant adsorb after, counter current drying, obtains powdered ingredients, by this powdered ingredients pelletizing forming in bowling machine, obtains the particle A that particle diameter is 1-2mm;
4) be that 3:1 mixes by Magnesium Silicate q-agent nano powder and nanometer colloidal particle weight ratio, prepare nanometer colloid coating agent; Wherein in Magnesium Silicate q-agent nano powder, mix feed grade titanic oxide nano, feed grade titanic oxide nano mixes by 1:9 weight ratio with Magnesium Silicate q-agent nano powder; Liquid-containing binder is mixed by sodium polyacrylate and xanthan gum weight ratio 6:1;
5) nanometer colloid coating agent certain mass step 4) prepared adopts spray pattern to be evenly coated on particle A surface, nanometer colloid coating agent and particle A weight ratio are 8:3, through facility for granulating pelletizing forming, obtain the spherical granules B that particle diameter is 1.5-3mm, i.e. sea farming substrate biological modification agent.The sea farming substrate biological modification agent that the present embodiment is obtained, even clad nano colloid coating agent outside component A.
Embodiment 7
A preparation method for sea farming substrate biological modification agent, comprises the following steps:
1) bacillus pumilus is inoculated in defined medium cultivates at 30 DEG C, then the bacillus pumilus under the normality obtained is cultivated respectively through domestication in the agar-agar soln of seawater content 10%, 30%, 50%, 90%, finally cultivate in the seawater of 100%, can growth and breeding in the seawater to genus bacillus.Finally to be able to seawater be the concentration of solute is 1*10
8~ 5*10
9the end of cfu/ml, changes engineering bacteria bacterium liquid;
Wherein, said defined medium details are: wheat bran 68.0g and soybean cake powder 35.0g adds 0.2%NaOH solution 1.0L, boils 1h, neutralize pH7 with HCl, then add agar 20.0g after mixing, after fusing, and packing sterilizing.
2) according to weight parts, by 93kg zeolite powder, 7kg part medical stone powder, 12kg montmorillonite Homogeneous phase mixing, then this mixture is carried out micronizing, obtain 650 object fixation support micro mists;
3) change by fixation support micro mist and the end that engineering bacteria bacteria liquid is long-pending mix than for 2:1, abundant adsorb after, counter current drying, obtains powdered ingredients, by this powdered ingredients pelletizing forming in bowling machine, obtains the particle A that particle diameter is 1.5-2mm;
4) be that 3:1 mixes by Magnesium Silicate q-agent nano powder and nanometer colloidal particle weight ratio, prepare nanometer colloid coating agent; Wherein in Magnesium Silicate q-agent nano powder, mix feed grade titanic oxide nano, feed grade titanic oxide nano mixes by 1:10 weight ratio with Magnesium Silicate q-agent nano powder; Liquid-containing binder is mixed by sodium polyacrylate and xanthan gum weight ratio 5:1;
5) nanometer colloid coating agent certain mass step 4) prepared adopts spray pattern to be evenly coated on particle A surface, nanometer colloid coating agent and particle A weight ratio are 7:3, through facility for granulating pelletizing forming, obtain the spherical granules B that particle diameter is 2-3.5mm, i.e. sea farming substrate biological modification agent.The sea farming substrate biological modification agent that the present embodiment is obtained, even clad nano colloid coating agent outside component A.
Embodiment 8
A preparation method for sea farming substrate biological modification agent, comprises the following steps:
1) subtilis is inoculated in defined medium cultivates at 30 DEG C, then the subtilis under the normality obtained is cultivated respectively through domestication in the agar-agar soln of seawater content 10%, 50%, 80%, finally cultivate in the seawater of 100%, can growth and breeding in the seawater to genus bacillus.Finally to be able to seawater be the concentration of solute is 5*10
8~ 1*10
9the end of cfu/ml, changes engineering bacteria bacterium liquid;
Wherein, said defined medium details are: wheat bran 50.0g and soybean cake powder 30.0g adds 0.2%NaOH solution 1.0L, boils 1h, neutralize pH7 with HCl, then add agar 25.0g after mixing, after fusing, and packing sterilizing.
2) according to weight parts, by 70kg zeolite powder, 10kg part medical stone powder, 13kg montmorillonite Homogeneous phase mixing, then this mixture is carried out micronizing, obtain 500 object fixation support micro mists;
3) change by fixation support micro mist and the end that engineering bacteria bacteria liquid is long-pending mix than for 3:1, abundant adsorb after, counter current drying, obtains powdered ingredients, by this powdered ingredients pelletizing forming in bowling machine, obtains the particle A that particle diameter is 1-2mm;
4) be that 3:1 mixes by Magnesium Silicate q-agent nano powder and nanometer colloidal particle weight ratio, prepare nanometer colloid coating agent; Wherein in Magnesium Silicate q-agent nano powder, mix feed grade titanic oxide nano, feed grade titanic oxide nano mixes by 1:12 weight ratio with Magnesium Silicate q-agent nano powder; Liquid-containing binder is mixed by sodium polyacrylate and xanthan gum weight ratio 5:1;
5) nanometer colloid coating agent certain mass step 4) prepared adopts spray pattern to be evenly coated on particle A surface, nanometer colloid coating agent and particle A weight ratio are 6:3, through facility for granulating pelletizing forming, obtain the spherical granules B that particle diameter is 1.5-3mm, i.e. sea farming substrate biological modification agent.The sea farming substrate biological modification agent that the present embodiment is obtained, even clad nano colloid coating agent outside component A.
Embodiment 9
A preparation method for sea farming substrate biological modification agent, comprises the following steps:
1) subtilis is inoculated in defined medium cultivates at 30 DEG C, then the subtilis under the normality obtained is cultivated respectively through domestication in the agar-agar soln of seawater content 10%, 30%, 80%, finally cultivate in the seawater of 100%, can growth and breeding in the seawater to genus bacillus.Finally to be able to seawater be the concentration of solute is 5*10
8the end of cfu/ml, changes engineering bacteria bacterium liquid;
Wherein, said defined medium details are: wheat bran 55.0g and soybean cake powder 40.0g adds 0.2%NaOH solution 1.0L, boils 1h, neutralize pH7 with HCl, then add agar 28.0g after mixing, after fusing, and packing sterilizing.
2) according to weight parts, by 100kg zeolite powder, 6kg part medical stone powder, 18kg montmorillonite Homogeneous phase mixing, then this mixture is carried out micronizing, obtain 600 object fixation support micro mists;
3) change by fixation support micro mist and the end that engineering bacteria bacteria liquid is long-pending mix than for 4:1, abundant adsorb after, counter current drying, obtains powdered ingredients, by this powdered ingredients pelletizing forming in bowling machine, obtains the particle A that particle diameter is 1-3mm;
4) be that 2:1 mixes by Magnesium Silicate q-agent nano powder and nanometer colloidal particle weight ratio, prepare nanometer colloid coating agent; Wherein in Magnesium Silicate q-agent nano powder, mix feed grade titanic oxide nano, feed grade titanic oxide nano mixes by 1:10 weight ratio with Magnesium Silicate q-agent nano powder; Liquid-containing binder is mixed by sodium polyacrylate and xanthan gum weight ratio 4:1;
5) nanometer colloid coating agent certain mass step 4) prepared adopts spray pattern to be evenly coated on particle A surface, nanometer colloid coating agent and particle A weight ratio are 2:1, through facility for granulating pelletizing forming, obtain the spherical granules B that particle diameter is 1.5-4mm, i.e. sea farming substrate biological modification agent.The sea farming substrate biological modification agent that the present embodiment is obtained, even clad nano colloid coating agent outside component A.
Below further illustrate beneficial effect of the present invention:
One, different fineness fixation support micro mist changes the contrast of engineering bacteria bacterium liquid absorption situation to the described end:
At the same temperature (seawater natural temperature), respectively quality is M
020-40 order (four parts) and 500 orders more than the fixation support micro mist of (four parts) put into seawater bacillus subtilis bacteria culture fluid, every 30min get successively a aqua containing fixation support micro mist carry out membrane filtration, claim absorption after weight M
1, according to the fixation support micro mist that calculates of poor quality to seawater bacillus subtilis bacteria culture fluid adsorptive capacity, adsorptive capacity=(M
1-M
0)/M
0* 100%.The fixation support micro mist of two kinds of different fineness soaks the adsorptive capacity after 30min, 60min, 90min and 120min respectively in table 1:
Table 1. different fineness fixation support micro mist is to seawater bacillus subtilis bacteria culture fluid isothermal adsorption amount %;
Obtained by table 1, the adsorptive capacity of fixation support micro mist more than granularity 500 order to seawater bacillus subtilis bacteria culture fluid is obviously greater than 20-40 object fixation support micro mist, and reason is the particle of more than granularity 500 order, and specific surface area is very big.In the inventive method, fixation support micro mist is crushed to more than 500 orders, greatly increases the absorption to seawater bacillus subtilis bacteria culture fluid, thus carry more subtilis, add the detergent power to seawater largely.
Two, control group and experimental group contrast seawater and the purge cases that cultivates substrate:
Choose the pond that 6 areas are 50 mu, the depth of water is 1.2 meters, and wherein 3 ponds as a control group (CK), and other 3 ponds are as experimental group (process A), and experimental session does not all change water, and every morning 11:00 gathers water sample and bed mud sample.
Control group (CK) pond bottom does not put sea farming substrate biological modification agent prepared by the inventive method;
The sea farming substrate biological modification agent that embodiment 3 is prepared according to the amount of 20kg/ mu by experimental group (process A) pond bottom is evenly spilt at the bottom of pond.
Conclusion:
1) as shown in Figure 2, the PH of control group (CK) breeding seawater is apparently higher than the PH of experimental group (process A) breeding seawater.PH value is the important indicator weighing seawater, and experimental group (process A) is obvious to seawater cleaning effect.
2) as shown in Figure 3, in control group (CK) breeding seawater, dissolved oxygen DO is starkly lower than the dissolved oxygen DO in experimental group (process A) breeding seawater.Dissolved oxygen DO is the important hydrochemistry factor of sea farming, and experimental group (process A) is obvious to seawater cleaning effect.
3) as shown in Figure 4, the chemical oxygen demand COD decline degree of control group (CK) breeding seawater is starkly lower than the decline degree of experimental group (process A) breeding seawater chemical oxygen demand COD.
4) as shown in Figure 5, experimental group (process A), control group (CK) substrate organic content all have rising when off-test, ascensional range is respectively 15.65%, 25.26%, show that the accumulation phenomenon of control group (CK) substrate organic matter is apparently higher than experimental group (process A), sea farming substrate biological modification of the present invention agent is excellent to sea farming substrate decontamination effect improving.
The foregoing is only preferred embodiment of the present invention, not in order to limit the present invention, within the spirit and principles in the present invention all, any amendment done, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (10)
1. a preparation method for sea farming substrate biological modification agent, is characterized in that: comprise the following steps:
1) the substratum domestication made through seawater by the genus bacillus under normality, purification, enable described genus bacillus growth and breeding in the seawater, obtain and change engineering bacteria bacterium liquid on earth;
2) according to weight parts, by 30 ~ 100 parts of zeolite powders, 3 ~ 15 parts of medical stone powders, 8 ~ 20 parts of montmorillonite Homogeneous phase mixing, then this mixture is carried out micronizing, obtain 500 order ~ 1000 object fixation support micro mists;
3) engineering bacteria bacteria liquid is changed by described fixation support micro mist and the described end long-pending than mixing for 1:1 ~ 5:1, fully after absorption, dry, granulate, obtain particle A;
4) be that 1:1 ~ 5:1 mixes by Magnesium Silicate q-agent nano powder and liquid adhesive weight ratio, prepare nanometer colloid coating agent;
5) described nanometer colloid coating agent is coated on described particle A surface, described nanometer colloid coating agent and described particle A weight ratio are 5:3 ~ 9:3, through facility for granulating pelletizing forming, obtain particle B, i.e. sea farming substrate biological modification agent.
2. the preparation method of sea farming substrate biological modification agent as claimed in claim 1, is characterized in that: the concentration that the described end changes engineering bacteria bacterium liquid is 1*10
7~ 5*10
9cfu/ml.
3. the preparation method of sea farming substrate biological modification agent as claimed in claim 1, is characterized in that: described genus bacillus is subtilis, slowly genus bacillus and Bacillus licheniformis.
4. sea farming substrate biological modification agent as claimed in claim 1, it is characterized in that: the bacillus living under described normality is cultivated through defined medium, these substratum details are: after wheat bran 50.0-80.0g and soybean cake powder 30.0-50.0g mixes, add 0.2-0.3%NaOH solution 1.0-1.5L, boil 1h, neutralize pH7 with HCl, then add agar 20.0-30.0g, after fusing, packing sterilizing.
5. the preparation method of sea farming substrate biological modification agent as claimed in claim 1, it is characterized in that: step 1) described in agar-agar soln or the full sea water of the substratum that makes of seawater to be seawater weight content be 10-99%, the genus bacillus under described normality is carried out domestication cultivation successively in seawater content sea water medium from low to high.
6. the preparation method of sea farming substrate biological modification agent as claimed in claim 1, it is characterized in that: step 2) in, according to weight parts, by 80 parts of zeolite powders, 8 parts of medical stone powders, 12 parts of montmorillonite Homogeneous phase mixing, then this mixture is carried out micronizing, obtain 500 object fixation support micro mists.
7. the preparation method of sea farming substrate biological modification agent as claimed in claim 1, is characterized in that: the liquid adhesive described in step 4) is mixed according to weight ratio 2:1 ~ 8:1 with xanthan gum by sodium polyacrylate.
8. the preparation method of sea farming substrate biological modification agent as claimed in claim 1, it is characterized in that: in the nano powder of Magnesium Silicate q-agent described in step 4), mix titanic oxide nano, described titanic oxide nano and described Magnesium Silicate q-agent nano powder weight ratio 1:12 ~ 1:8; Step 5) in, described nanometer colloid coating agent adopts spray pattern to be evenly coated on described particle A surface.
9. the preparation method of sea farming substrate biological modification agent as claimed in claim 1, is characterized in that: described nanometer colloid coating agent and described particle A weight ratio are 7:3.。
10. the sea farming substrate biological modification agent utilizing the preparation method of the sea farming substrate biological modification agent described in any one of claim 1-9 to obtain, is characterized in that: clad nano colloid coating agent outside described particle A.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106186347A (en) * | 2016-07-12 | 2016-12-07 | 山东胜伟园林科技有限公司 | A kind of used in mariculture desulfurated plaster and preparation method thereof |
| CN112741231A (en) * | 2021-01-20 | 2021-05-04 | 广西民族师范学院 | Probiotic-based antibiotic-free eel feed and preparation method thereof |
| CN112741230A (en) * | 2021-01-20 | 2021-05-04 | 广西民族师范学院 | Compound feed for improving survival rate and weight gain rate of eel in high temperature period and preparation method thereof |
| CN114212957A (en) * | 2021-11-23 | 2022-03-22 | 重庆誉康欣生物技术有限公司 | Formula and production process for decomposing bottom modification |
-
2013
- 2013-09-22 CN CN201310434778.9A patent/CN104450666B/en active Active
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106186347A (en) * | 2016-07-12 | 2016-12-07 | 山东胜伟园林科技有限公司 | A kind of used in mariculture desulfurated plaster and preparation method thereof |
| CN112741231A (en) * | 2021-01-20 | 2021-05-04 | 广西民族师范学院 | Probiotic-based antibiotic-free eel feed and preparation method thereof |
| CN112741230A (en) * | 2021-01-20 | 2021-05-04 | 广西民族师范学院 | Compound feed for improving survival rate and weight gain rate of eel in high temperature period and preparation method thereof |
| CN114212957A (en) * | 2021-11-23 | 2022-03-22 | 重庆誉康欣生物技术有限公司 | Formula and production process for decomposing bottom modification |
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