CN104381697A - Microecological preparation used in prawn feeds and application thereof - Google Patents
Microecological preparation used in prawn feeds and application thereof Download PDFInfo
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- CN104381697A CN104381697A CN201410719316.6A CN201410719316A CN104381697A CN 104381697 A CN104381697 A CN 104381697A CN 201410719316 A CN201410719316 A CN 201410719316A CN 104381697 A CN104381697 A CN 104381697A
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Abstract
The invention provides a microecological preparation used in prawn feeds and an application thereof. The microecological preparation comprises the following ingredients: bacillus subtilis powder, bacillus licheniformis powder, a honeysuckle flower extract (10 to 1) and astragalus polysaccharides (60%) in the mass ratio of 5 to 3 to 1 to 1. By adding the microecological preparation to the prawn feeds, the growth and the anti-stress capacities of prawns can be improved, the immune functions can be enhanced and the survival rates of the prawns can be increased.
Description
Technical field
The invention belongs to prawn feed technical field, be specifically related to a kind of probiotics for prawn feed and application thereof.
Background technology
Along with the expansion of prawn seed-rearing, cultivation, disease becomes the key factor that restriction aquaculture develops in a healthy way.Long-Time Service antibiotic causes pathogenic bacteria drug resistance to strengthen, and cultivated animals immunity reduces, and finding antibiotic substitute is current research emphasis.
Probio has maintenance gut flora balance, strengthens the effect such as immunity of organism and premunition; Chinese medicine contains various active composition, has the features such as antibacterial, antiviral, noresidue.There is certain synergy between probio and Chinese medicine, probio can promote absorbing of Chinese medicine, and some composition of Chinese medicine has facilitation to bacterial growth metabolism, can promote the propagation of probio; Both reasonable employment, can reach both collaborative optimum efficiencies used.
Summary of the invention
The object of the present invention is to provide a kind of probiotics for prawn feed and application thereof, this probiotics can add in prawn basal feed, has the effect promoting prawn growth, improve prawn premunition.
The present invention also aims to provide probiotics preparing the application in prawn feed.
For achieving the above object, the present invention adopts following technical proposals to be achieved:
For a probiotics for prawn feed, bacillus subtilis bacterium powder, Bacillus licheniformis powder, Honegsukle flower P.E, astragalus polyose form.
In described probiotics, the quality proportioning of each component is withered grass: lichens: honeysuckle: astragalus polyose=5:3:1:1; Bacillus subtilis bacterium amount >3 × 10
9cFU/g, Bacillus licheniformis amount >1 × 10
9cFU/g.
Present invention also offers described probiotics and preparing the application in prawn feed, the addition of described probiotics is 0.5 ‰-2 ‰ of prawn basal feed quality.
Bacillus has stronger protease, lipase, diastatic activity, improves efficiency of feed utilization; Bacillus subtilis has very strong inhibitory action to vibrios, Escherichia coli and baculoviral etc.; Stimulate the growth of aquatic livestock immune organ, strengthen immunity of organisms; Reduce shrimp disease, improve prawn output.
Honegsukle flower P.E main component is chlorogenic acid, has obvious inhibitory action to vibrio parahaemolytious, Aeromonas hydrophila; Astragalus polyose has effects such as strengthening function of immune system and antiviral, resisting stress, protect the liver.
Compared with prior art, advantage of the present invention and technique effect are: on the basis of existing probiotics, composite Chinese medical extract, and both synergies can effectively promote that prawn grows, and suppress pathogen, strengthen immunocompetence, improve prawn survival rate.
Accompanying drawing explanation
Prawn PAS (ACP) determination of activity in Fig. 1 embodiment of the present invention 2.
Prawn serum alkaline phosphatase (AKP) determination of activity in Fig. 2 embodiment of the present invention 2.
Prawn serum superoxide dismutases (SOD) determination of activity in Fig. 3 embodiment of the present invention 2.
Gut of shrimp protease activity determination in Fig. 4 embodiment of the present invention 2.
In Fig. 5 embodiment of the present invention 2, gut of shrimp amylase activity measures.
Detailed description of the invention
In conjunction with following specific embodiment, the present invention is further detailed explanation in the present invention.
The preparation of embodiment 1 probiotics feed
Probiotics for prawn feed described in the present embodiment comprises: bacillus subtilis 4 × 10
9cFU/g, bacillus licheniformis 1 × 10
9cFU/g, Honegsukle flower P.E (10:1), astragalus polyose (60%).
Probiotics of the present invention is preparing the application in prawn feed: the addition of described probiotics is 1 ‰ of feed gross weight.Detecting bacillus subtilis after feed granulating is 2.4 × 10
8cFU/g, bacillus licheniformis 3.8 × 10
7cFU/g, extractive content does not detect.
Embodiment 2, probiotics feed prawn application test
Test aquarium volume is 60L, uninterruptedly inflates during cultivation.Test is divided into 2 groups, and control group (basal feed) and test group (probiotics feed), often organize 5 repetitions.Each aquarium cultivates 5 tail Penaeus Vannmeis.Select healthy Penaeus Vannmei 50 tail like body weight, body appearance before test, average weight (8.6 ± 0.1) g, divide at random with 10 aquariums, start test after raising and train 1 week, feeding cycle is 40d.Weigh after off-test; Blood sampling measures serum superoxide dismutases (SOD), alkaline phosphatase (AKP), acid phosphatase (ACP) activity; Get intestinal samples and measure protease, amylase; Kit is purchased from Nanjing and builds up Bioengineering Research Institute.
Table 1 Penaeus Vannmei body weight increase rate
| Group | Initial weight | End is heavy | Growth rate % |
| Test group | 43.62±2.13 | 85.59±3.27 | 96.22±3.42 a |
| Control group | 44.28±3.59 | 76.09±5.91 | 71.84±1.02 |
As shown in table 1, test group prawn body weight growth rate is 96.22%, be significantly higher than control group (
p<0.05), illustrate that this probiotics has certain facilitation to prawn growth.
Phosphatase is the enzyme of the various phosphorus-containing compound hydrolysis of a kind of energy catalysis, is removing toxic substances system important in animal body, comprises acid phosphatase (ACP) and alkaline phosphatase (AKP).Superoxide dismutase (SOD) is a kind of important oxygen free radical scavenger in body, can oxygen radical in balanced body.As can be seen from Fig. 1-3, add ACP, AKP, SOD of prawn serum after probiotics and all increase, and ACP, AKP activity be significantly higher than control group (
p<0.05).
Add protease, the amylase activity (see Fig. 4, Fig. 5) that can improve enteron aisle after probiotics in various degree, and then improve efficiency of feed utilization, wherein proteinase activity be significantly higher than control group (
p<0.05).
In sum, probiotics can promote that prawn grows, and has certain immunoregulation effect, can improve prawn serum ascites albumin gradient, AKP, SOD are active.
Above embodiment only in order to technical scheme of the present invention to be described, but not is limited; Although with reference to previous embodiment to invention has been detailed description, for the person of ordinary skill of the art, still can modify to the technical scheme described in previous embodiment, or equivalent replacement is carried out to wherein portion of techniques feature; And these amendments or replacement, do not make the essence of appropriate technical solution depart from the spirit and scope of the present invention's technical scheme required for protection.
Claims (4)
1. for a probiotics for prawn feed, it is characterized in that: it is made up of bacillus subtilis bacterium powder, Bacillus licheniformis powder, Honegsukle flower P.E, astragalus polyose.
2. a kind of probiotics for prawn feed according to claim 1, is characterized in that: in described probiotics, the quality proportioning of each component is withered grass: lichens: honeysuckle: astragalus polyose=5:3:1:1.
3. a kind of probiotics for prawn feed according to claim 1, is characterized in that: bacillus subtilis bacterium amount >3 × 10
9cFU/g, Bacillus licheniformis amount >1 × 10
9cFU/g.
4. probiotics according to claim 1 and 2 is preparing the application in prawn feed, it is characterized in that: the addition of described probiotics is 0.5 ‰-2 ‰ of prawn basal feed quality.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410719316.6A CN104381697A (en) | 2014-12-03 | 2014-12-03 | Microecological preparation used in prawn feeds and application thereof |
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| CN201410719316.6A CN104381697A (en) | 2014-12-03 | 2014-12-03 | Microecological preparation used in prawn feeds and application thereof |
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| CN104381697A true CN104381697A (en) | 2015-03-04 |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104738314A (en) * | 2015-04-27 | 2015-07-01 | 清远海贝生物技术有限公司 | Transportation stress resisting Chinese herbal medicinal probiotics for penaeus vannamei boone as well as preparation method and application of Chinese herbal medicinal probiotics |
| CN105053514A (en) * | 2015-07-24 | 2015-11-18 | 河南仰韶生化工程有限公司 | Compound microbial feed additive and production method thereof |
| CN106550915A (en) * | 2016-11-11 | 2017-04-05 | 山东省海洋生物研究院 | A kind of microorganism regulation and control method of japonicus nursery |
| CN107950755A (en) * | 2017-11-23 | 2018-04-24 | 安徽金牧饲料有限公司 | A kind of organic shrimp feed of environmental protection and preparation method thereof |
| CN108514045A (en) * | 2018-03-12 | 2018-09-11 | 大连民族大学 | One kind having purification of water quality and the immunocompetent fancy carp microbial inoculum bait of raising and preparation method thereof |
| CN109463576A (en) * | 2018-12-30 | 2019-03-15 | 盐城工学院 | It is a kind of improve prawn survival rate additive and its application |
| CN114794331A (en) * | 2022-04-13 | 2022-07-29 | 扬州大学 | Composite micro-ecological bacteria agent for improving immunity of aquatic animals and preparation method thereof |
| CN114869963A (en) * | 2022-05-25 | 2022-08-09 | 河南省帝一方生物制药有限公司 | SOD enzyme fermentation preparation for oxidation resistance and stress resistance of livestock and poultry and preparation method thereof |
-
2014
- 2014-12-03 CN CN201410719316.6A patent/CN104381697A/en active Pending
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104738314A (en) * | 2015-04-27 | 2015-07-01 | 清远海贝生物技术有限公司 | Transportation stress resisting Chinese herbal medicinal probiotics for penaeus vannamei boone as well as preparation method and application of Chinese herbal medicinal probiotics |
| CN104738314B (en) * | 2015-04-27 | 2017-09-12 | 清远海贝生物技术有限公司 | Chinese herbal microecological preparation of the anti-transport stress of Penaeus Vannmei and its preparation method and application |
| CN105053514A (en) * | 2015-07-24 | 2015-11-18 | 河南仰韶生化工程有限公司 | Compound microbial feed additive and production method thereof |
| CN106550915A (en) * | 2016-11-11 | 2017-04-05 | 山东省海洋生物研究院 | A kind of microorganism regulation and control method of japonicus nursery |
| CN106550915B (en) * | 2016-11-11 | 2020-11-03 | 山东省海洋生物研究院 | Microbial regulation and control method for raising seedlings of penaeus japonicus |
| CN107950755A (en) * | 2017-11-23 | 2018-04-24 | 安徽金牧饲料有限公司 | A kind of organic shrimp feed of environmental protection and preparation method thereof |
| CN108514045A (en) * | 2018-03-12 | 2018-09-11 | 大连民族大学 | One kind having purification of water quality and the immunocompetent fancy carp microbial inoculum bait of raising and preparation method thereof |
| CN108514045B (en) * | 2018-03-12 | 2021-08-13 | 大连民族大学 | Koi microbial inoculum bait with water quality purification and immunocompetence improvement functions and preparation method thereof |
| CN109463576A (en) * | 2018-12-30 | 2019-03-15 | 盐城工学院 | It is a kind of improve prawn survival rate additive and its application |
| CN114794331A (en) * | 2022-04-13 | 2022-07-29 | 扬州大学 | Composite micro-ecological bacteria agent for improving immunity of aquatic animals and preparation method thereof |
| CN114869963A (en) * | 2022-05-25 | 2022-08-09 | 河南省帝一方生物制药有限公司 | SOD enzyme fermentation preparation for oxidation resistance and stress resistance of livestock and poultry and preparation method thereof |
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