CN104350948A - Method for culturing mushroom cultivated species - Google Patents
Method for culturing mushroom cultivated species Download PDFInfo
- Publication number
- CN104350948A CN104350948A CN201410647963.0A CN201410647963A CN104350948A CN 104350948 A CN104350948 A CN 104350948A CN 201410647963 A CN201410647963 A CN 201410647963A CN 104350948 A CN104350948 A CN 104350948A
- Authority
- CN
- China
- Prior art keywords
- mushroom
- days
- liquid
- culturing
- strain
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/40—Fertilisers incorporated into a matrix
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Mycology (AREA)
- Environmental Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Mushroom Cultivation (AREA)
Abstract
The invention discloses a method for culturing mushroom cultivated species. The method comprises the steps of firstly, culturing a liquid mushroom strain for 3-4 days; producing a particle culture medium by using dry feces, soil, cross-linked sodium polyacrylate, starch coagulant and the like; directly putting the liquid mushroom strain in sterilized particle culture medium for culturing for 7-10 days, thus preparing a mushroom liquid-particle strain, wherein the whole seed production period is 10-14 days. The method for culturing the mushroom cultivated species, disclosed by the invention, is suitable for seed production of double-spore mushroom, four-spore mushroom and spring mushroom and is also suitable for the seed production of oyster mushroom and shiitake mushroom.
Description
Technical field
The present invention relates to a kind of cultural method of mushroom cultivating seed.
Background technology
The mushroom of most countries all adopts grain spawn in the world now, also has a few countries to adopt excrement grass bacterial classification.The use of grain spawn is being bottled, digging bottle and is being sowed all than excrement grass bacterial classification conveniently.Grain spawn energy mixed seeding is simultaneously in planting material, and strain development point is many, and mycelia healing is fast, and output is also high.But grain spawn is the same with excrement grass bacterial classification, and be also rely on constantly spreading and breeding of vaccination mycelia, incubation time reaches 42 ~ 45 days, and cell age differs.Top mycelia is easily old and feeble, and microbiological contamination rate is high.
Summary of the invention
Technical problem to be solved by this invention is: provide a kind of production of hybrid seeds time short, strain development point is many, and mycelia healing is fast, the cultural method of the mushroom cultivating seed that output is also high.
For solving the problems of the technologies described above, the technical solution used in the present invention is: a kind of cultural method of mushroom cultivating seed, taking liquid fungus culture as original seed, in the particulate media directly after access sterilizing, be trained mushroom liquid---granular bacteria strain, the whole production of hybrid seeds cycle is 9 ~ 12 days.
Preferably, particulate media is made up of cross linked sodium polyacrylate, argol, earth, starch coagulating agent.
Preferably, the deep drainpipe time of liquid fungus culture is 3 ~ 4 days.It is 7 ~ 10 days that liquid fungus culture directly accesses the incubation time after the particulate media after sterilizing.
The invention has the beneficial effects as follows: the present invention adopts deep drainpipe mycelium to prepare the cultural method of mushroom cultivating seed, is a kind of fast method for preparing of mushroom.Mushroom liquid is prepared by the method---particle cultivation strain, the whole production of hybrid seeds time only needs 10 ~ 14 days, faster than the mushroom producing method for seed of routine more than 5 times.With mushroom cultivating seed prepared by the method, water suction moisture retention is strong, can adapt in the composts or fertilisers of cultivating of direct mixed seeding after secondary fermentation, strain development point is many, and mycelium germination power is strong, and mycelia healing early, output improves 16% than conventional bacterial classification, is applicable to the production of hybrid seeds needs of commerial growing mushroom.
Embodiment
Embodiment 1:
The agaricus bisporus bacterial strain access mushroom liquid culture medium A after domestication, at 25 DEG C, deep drainpipe 3 ~ 4 days, can be trained liquid fungus culture.Liquid fungus culture is directly accessed in the particulate media A after sterilizing, cultivates at 23 DEG C and just make mushroom liquid in 7 ~ 10 days---particle cultivation strain.
Wherein the formula of mushroom liquid culture medium A is:
Soy sauce 5g, yeast extract powder 1g, peptone 1g, maltose 20g, glucose 10g, rape oil 3g, corn flour 10g, calcium carbonate 2g, add water to 1000ml, and PH is 7.0.
The formula of particulate media A is:
Argol 45g, dry clay 45g, flour 10g, calcium carbonate 1g, calcium sulphate 1g, cross linked sodium polyacrylate 0.5g, water 45ml.
Embodiment 2:
The agaricus bisporus bacterial strain access mushroom liquid medium B after domestication, at 23 DEG C, deep drainpipe 3 ~ 4 days, can be trained liquid fungus culture.Liquid fungus culture is directly accessed in the particulate media B after sterilizing, cultivates at 22 DEG C and just make mushroom liquid in 7 ~ 10 days---particle cultivation strain.
Wherein the formula of mushroom liquid culture medium A is:
Yeast extract 1g, peptone 2g, maltose 5g, thiamine 100mg, potassium hydrogen phosphate 0.5g, magnesium sulfate 0.5g, wheat bran liquor with 5% add to 1000ml, and PH is 7.0.
The formula of particulate media A is:
Argol 45g, dry clay 45g, flour 5g, corn flour 5g, calcium carbonate 0.2g, calcium sulphate 12g, cross linked sodium polyacrylate 0.5g, water 40ml.
Claims (3)
1. a cultural method for mushroom cultivating seed, is characterized in that: take liquid fungus culture as original seed, is trained mushroom liquid in the particulate media directly after access sterilizing---granular bacteria strain, the whole production of hybrid seeds cycle is 9 ~ 12 days.
2. the cultural method of mushroom cultivating seed according to claim 1, is characterized in that: described particulate media is made up of cross linked sodium polyacrylate, argol, earth, starch coagulating agent.
3. the cultural method of mushroom cultivating seed according to claim 1, is characterized in that: the deep drainpipe time of described liquid fungus culture is 3 ~ 4 days.It is 7 ~ 10 days that liquid fungus culture directly accesses the incubation time after the particulate media after sterilizing.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410647963.0A CN104350948A (en) | 2014-11-14 | 2014-11-14 | Method for culturing mushroom cultivated species |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410647963.0A CN104350948A (en) | 2014-11-14 | 2014-11-14 | Method for culturing mushroom cultivated species |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104350948A true CN104350948A (en) | 2015-02-18 |
Family
ID=52518345
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410647963.0A Pending CN104350948A (en) | 2014-11-14 | 2014-11-14 | Method for culturing mushroom cultivated species |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104350948A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105340580A (en) * | 2015-12-01 | 2016-02-24 | 仇颖超 | Method for cultivating agaricus bisporus through liquid strains |
| CN105766373A (en) * | 2016-03-30 | 2016-07-20 | 重庆三零三科技有限公司 | High-temperature agaricus campestris cultivation method |
| CN106171534A (en) * | 2016-09-08 | 2016-12-07 | 莘县富邦菌业有限公司 | A kind of cultural method of cultivation of agaricus bisporus kind |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN86100509A (en) * | 1986-02-04 | 1986-07-30 | 上海师范大学 | A kind of technology with deep culturing mycelium for mushroom breeding |
| JP2005261377A (en) * | 2004-03-22 | 2005-09-29 | Nippon Kinoko Kenkyusho | Method for culturing mushroom bed of mushroom |
| CN102369838A (en) * | 2010-08-11 | 2012-03-14 | 宝生物工程株式会社 | Manufacturing Method Of Liquid Seed Fungus Of Mushroom |
| CN102612994A (en) * | 2012-03-28 | 2012-08-01 | 何寒 | Method for cultivating oyster mushroom cultivation by directly mixing liquid spawns with raw materials |
-
2014
- 2014-11-14 CN CN201410647963.0A patent/CN104350948A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN86100509A (en) * | 1986-02-04 | 1986-07-30 | 上海师范大学 | A kind of technology with deep culturing mycelium for mushroom breeding |
| JP2005261377A (en) * | 2004-03-22 | 2005-09-29 | Nippon Kinoko Kenkyusho | Method for culturing mushroom bed of mushroom |
| CN102369838A (en) * | 2010-08-11 | 2012-03-14 | 宝生物工程株式会社 | Manufacturing Method Of Liquid Seed Fungus Of Mushroom |
| CN102612994A (en) * | 2012-03-28 | 2012-08-01 | 何寒 | Method for cultivating oyster mushroom cultivation by directly mixing liquid spawns with raw materials |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105340580A (en) * | 2015-12-01 | 2016-02-24 | 仇颖超 | Method for cultivating agaricus bisporus through liquid strains |
| CN105766373A (en) * | 2016-03-30 | 2016-07-20 | 重庆三零三科技有限公司 | High-temperature agaricus campestris cultivation method |
| CN106171534A (en) * | 2016-09-08 | 2016-12-07 | 莘县富邦菌业有限公司 | A kind of cultural method of cultivation of agaricus bisporus kind |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103539535B (en) | Active biological matrix product specially used for culture of cucumber seedlings | |
| CN1843073B (en) | Facility vegetable mycorrhiza production method | |
| CN105684847B (en) | A kind of watermelon seedling culturing substrate and preparation method thereof | |
| CN103190292B (en) | Forest cultivation method of morchella crassipes | |
| CN102613054B (en) | Method for improving cold and disease resistance of tobacco | |
| CN109136149B (en) | Application of the bacillus subtilis in terms of soil phosphorus decomposing and cellulose degradation | |
| CN102827794A (en) | Pseudomonas mediterranea strain and application thereof | |
| CN103159570A (en) | Solid microbial fertilizer and preparation method thereof | |
| CN103493685A (en) | Method for cultivating phellinus igniarius by using wild living tree stumps | |
| CN104350948A (en) | Method for culturing mushroom cultivated species | |
| CN105993530A (en) | Method for interplanting high-temperature type edible mushrooms with sugarcane | |
| CN104521562A (en) | Cultivation method for repeated oyster mushroom fruiting | |
| CN102487723B (en) | Method for industrially producing bottle-cultivated or bag-cultivated lentinus giganteus berk without earthing | |
| CN104230459A (en) | Preparation method for agrocybe aegirit nutritional medium | |
| CN104250121A (en) | Method for preparing Jishurong nutrient medium | |
| CN104250167A (en) | Agaricus blazei Murill nutritional medium preparation method | |
| CN107027518A (en) | A kind of method that pig manure slag batch cultivates flat mushroom | |
| CN104250163A (en) | Agaricus blazei Murill nutritional medium preparation method | |
| CN104250159A (en) | Method for preparing Jishurong nutrient medium | |
| CN104250164A (en) | Agaricus blazei Murill nutritional medium preparation method | |
| CN104250169A (en) | Agaricus blazei Murill nutritional medium preparation method | |
| CN104250124A (en) | Agaricus blazei Murill nutritional medium preparation method | |
| CN104250165A (en) | Agaricus blazei Murill nutritional medium preparation method | |
| CN104230467A (en) | Preparation method for agrocybe aegirit nutritional medium | |
| CN104250168A (en) | Agaricus blazei Murill nutritional medium preparation method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20150218 |