CN104324058A

CN104324058A - Preparation method of ganoderma lucidum spores oil

Info

Publication number: CN104324058A
Application number: CN201310623484.0A
Authority: CN
Inventors: 钟延华
Original assignee: Individual
Current assignee: Individual
Priority date: 2013-11-30
Filing date: 2013-11-30
Publication date: 2015-02-04

Abstract

The invention discloses a preparation method of ganoderma lucidum spores oil, and belongs to the technical field of extraction of effective components from medicinal plants. The preparation method of the ganoderma lucidum spores oil comprises the steps of breaking cell walls of ganoderma lucidum spores by using an enzymolysis method; then carrying out wet granulation by using water as a solvent; extracting with supercritical CO2; centrifuging and refining to obtain a light yellow oily material being the ganoderma lucidum spores oil. The preparation method overcomes the problems that active substances in the spores are difficult to extract due to double-layer cell walls of the ganoderma lucidum spores. With the preparation method provided by the invention, the yield of the ganoderma lucidum spores oil is higher than 30%.

Description

The preparation method of Ganoderma spore oil

Technical field

The present invention relates to active ingredient of autonomic drug extractive technique field, especially a kind of preparation method of Ganoderma spore oil.

Background technology

Ganoderma spore is the dry spore of Polyporaceae (Ployporaceae) Ganoderma Ganoderma Lucium (Ley S.ex Fr.) Karest, for collecting when tame Ganoderma bullet puts spore, drying and obtaining.Ganoderma spore has whole genetic activities materials of Ganoderma, is the elite of Ganoderma.Ganoderma spore oil extracts from Ganoderma spore, and its main component is triterpenes Ganodenic acid, unsaturated fatty acid, organic germanium and a small amount of ganoderan.

Ganoderma spore has double-layer cell wall, its main component is chitin, lignin and cellulose, Si, Ca, Fe, Mg, Al etc., make sporoderm very solid hard, can acidproof, alkaline-resisting, withstand voltage, heatproof, also highly stable to digestive enzyme, in spore, active substance is wrapped and not easily extracts and be prepared into Ganoderma spore oil.

Summary of the invention

The object of this invention is to provide one can be quick, the preparation method of efficient Ganoderma spore oil, solve because Ganoderma spore has the existence of double-layer cell wall and make the problem that active substance in spore is not easily extracted, the yield of its Ganoderma spore oil reaches more than 30%.

The technical scheme of technical solution problem of the present invention is:

A preparation method for Ganoderma spore oil, comprises the following steps:

Being raw material with Ganoderma spore, through enzymatic shell-broken, take water as solvent wet granulation, supercritical CO ₂after extraction, centrifuge refining, obtain pale yellow oil i.e. this Ganoderma spore oil;

Wherein, described enzymatic shell-broken process is specially: first Ganoderma spore is immersed in 75% ethanol the 15min ~ 20min that carries out disinfection, and then puts into enzyme liquid enzymolysis 18h ~ 24h; Described enzyme digestion reaction carries out at dark place; The temperature of described enzyme digestion reaction is 28 DEG C ~ 31 DEG C; Described enzyme liquid cellulase by account for enzyme liquid gross mass 2% ~ 5%, sucrose account for enzyme liquid gross mass 12% ~ 16%, inorganic salt accounts for 0.5% ~ 0.8% of enzyme liquid gross mass, remaining is made up of buffer.

Owing to adopting technique scheme, the beneficial effect that the present invention obtains is:

The cell wall of Ganoderma spore in the present invention, does not need to adopt Mechanical Crushing, saves and buy the expense of main equipment and the human and material resources spent by Mechanical Crushing, but adopt enzymatic isolation method to destroy its cell wall, cost is low, effective, and the yield of its Ganoderma spore oil reaches more than 30%.

Detailed description of the invention

Below in conjunction with specific embodiment, the invention will be further described:

Embodiment 1

The preparation method of Ganoderma spore oil comprises the following steps:

1Kg Ganoderma spore is immersed in 75% ethanol the 15min that carries out disinfection, put into by the cellulase accounting for enzyme liquid gross mass 2% again, account for the sucrose of enzyme liquid gross mass 12%, account for the inorganic salt of enzyme liquid gross mass 0.5%, to account in the enzyme liquid of the buffer composition of enzyme liquid gross mass 85.5% and to be placed in dark place, be enzymolysis 18h under the condition of 28 DEG C in temperature, then filter and be solvent wet granulation with water, supercritical CO ₂after extraction, centrifuge refining, obtain 0.31Kg pale yellow oil i.e. this Ganoderma spore oil.

In the present embodiment, the yield of Ganoderma spore oil is 31%.

Embodiment 2

The preparation method of Ganoderma spore oil comprises the following steps:

1Kg Ganoderma spore is immersed in 75% ethanol the 20min that carries out disinfection, put into by the cellulase accounting for enzyme liquid gross mass 3% again, account for the sucrose of enzyme liquid gross mass 14%, account for the inorganic salt of enzyme liquid gross mass 0.6%, to account in the enzyme liquid of the buffer composition of enzyme liquid gross mass 82.4% and to be placed in dark place, be enzymolysis 124h under the condition of 31 DEG C in temperature, then filter and be solvent wet granulation with water, supercritical CO ₂after extraction, centrifuge refining, obtain 0.35Kg pale yellow oil i.e. this Ganoderma spore oil.

In the present embodiment, the yield of Ganoderma spore oil is 35%.

Embodiment 3

The preparation method of Ganoderma spore oil comprises the following steps:

1Kg Ganoderma spore is immersed in 75% ethanol the 18min that carries out disinfection, put into by the cellulase accounting for enzyme liquid gross mass 5% again, account for the sucrose of enzyme liquid gross mass 16%, account for the inorganic salt of enzyme liquid gross mass 0.8%, to account in the enzyme liquid of the buffer composition of enzyme liquid gross mass 78.2% and to be placed in dark place, be enzymolysis 21h under the condition of 30 DEG C in temperature, then filter and be solvent wet granulation with water, supercritical CO ₂after extraction, centrifuge refining, obtain 0.404Kg pale yellow oil i.e. this Ganoderma spore oil.

In the present embodiment, the yield of Ganoderma spore oil is 40.4%.

Claims

1. a preparation method for Ganoderma spore oil, comprising with Ganoderma spore is raw material, through enzymatic shell-broken, take water as solvent wet granulation, supercritical CO ₂after extraction, centrifuge refining, obtain pale yellow oil i.e. this Ganoderma spore oil; It is characterized in that described enzymatic shell-broken process is specially: first Ganoderma spore is immersed in 75% ethanol the 15min ~ 20min that carries out disinfection, and then put into enzyme liquid enzymolysis 18h ~ 24h; Described enzyme digestion reaction carries out at dark place; The temperature of described enzyme digestion reaction is 28 DEG C ~ 31 DEG C; Described enzyme liquid cellulase by account for enzyme liquid gross mass 2% ~ 5%, sucrose account for enzyme liquid gross mass 12% ~ 16%, inorganic salt accounts for 0.5% ~ 0.8% of enzyme liquid gross mass, remaining is made up of buffer.