CN104307841B - A kind of low cost kills the method that house refuse infects pathogeny - Google Patents

A kind of low cost kills the method that house refuse infects pathogeny Download PDF

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CN104307841B
CN104307841B CN201410434224.3A CN201410434224A CN104307841B CN 104307841 B CN104307841 B CN 104307841B CN 201410434224 A CN201410434224 A CN 201410434224A CN 104307841 B CN104307841 B CN 104307841B
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bacteria liquid
composite bacteria
rubbish
low cost
house refuse
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CN104307841A (en
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林小明
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Guangdong spring ecological science and Technology Group Co., Ltd.
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GUANGDONG YUANSHENG ECOLOGICAL ENVIRONMENT PROTECTION TECHNOLOGY Co Ltd
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Abstract

The present invention relates to waste disposal method, be specifically related to a kind of low cost and kill the method that house refuse infects pathogeny, the aeration tower of several tapers is built in garbage pool, rubbish can be deposited in above aeration tower, the bottom in rubbish pond arranges several injector heads, and first above rubbish heap, spray, containing the first composite bacteria liquid, then sprays the second composite bacteria liquid by injector head to rubbish heap after 2-4 hour, rubbish heap fermentation produces heat, rubbish heap temperature 60-80 DEG C; Described first composite bacteria liquid includes bacillus subtilis, bacillus licheniformis, VT bacterium; Described second composite bacteria liquid comprises streptomyces cellulosae, ammonia nitrogen degradation bacterium, Thermophilic Bacteria, and it adopts the mode of biological bacteria aerobic fermentation to produce heat, and rubbish heap temperature is raised, reach the object killed and infect pathogeny, have cost low, environmental pollution is little, the feature that treatment cycle is short.

Description

A kind of low cost kills the method that house refuse infects pathogeny
Technical field
The present invention relates to waste disposal method, be specifically related to a kind of low cost and kill the method that house refuse infects pathogeny.
Background technology
Rubbish is the waste product that human lives certainly leads to, and China produces rubbish up to ten million ton every day, even if the cleaning in time of these rubbish is to large-scale soot.In short supply along with land resource, the landfill of house refuse is more and more near the residence of resident, and rubbish produces stench, and can produce more infection pathogeny, to the life of the mankind with healthyly form larger threat.Current is generally take the sterilization of spray medicine to the processing mode of rubbish, or compost etc., the cost not only processed is high, and environmental pollution is serious, and the time span of compost is very long, cannot process the rubbish increased day by day every day in time.
Summary of the invention
The object of the invention is for deficiency of the prior art, a kind of low cost is provided to kill the method for house refuse infection pathogeny, it adopts the mode of biological bacteria aerobic fermentation to produce heat, rubbish heap temperature is raised, reach the object killed and infect pathogeny, have cost low, environmental pollution is little, the feature that treatment cycle is short.
Object of the present invention is achieved through the following technical solutions:
A kind of low cost kills the method that house refuse infects pathogeny, the aeration tower of several tapers is built in garbage pool, rubbish can be deposited in above aeration tower, the bottom in rubbish pond arranges several injector heads, first above rubbish heap, spray contains the first composite bacteria liquid, then spray the second composite bacteria liquid by injector head to rubbish heap after 2-4 hour, rubbish heap fermentation produces heat, rubbish heap temperature 60-80 DEG C; Described first composite bacteria liquid includes bacillus subtilis, bacillus licheniformis, VT bacterium; Described second composite bacteria liquid comprises streptomyces cellulosae, ammonia nitrogen degradation bacterium, Thermophilic Bacteria.The deposit number of cellulose strepto-is 4.381, is preserved in China General Microbiological culture presevation administrative center, from Institute of Microorganism, Academia Sinica.
When first spraying the first composite bacteria liquid, make the surface uniform of rubbish heap cover upper first composite bacteria liquid, and slowly penetrate in rubbish heap, ferment gradually.Then upwards spray rubbish by the injector head of garbage pool to rubbish heap, the second composite bacteria liquid is penetrated in rubbish heap further, strengthen ferment effect.And after jetted the first composite bacteria liquid, the temperature of rubbish heap raises gradually, then when spraying the second composite bacteria liquid, its ferment effect is more obvious, also rapider.Biological bacteria activity when temperature is lower of the second composite bacteria liquid is low, and the time has grown the easy inactivation of meeting, thus needs separately spray.
The subtilin produced in bacillus subtilis thalli growth process, polymyxins, nystatin, gramicidins isoreactivity material, the conditioned pathogen of these active materials to pathogenic bacteria or autogenous infection has obvious inhibitory action.Directly commercially availablely buy, bacillus subtilis buying of the present invention is to North Sea Qun Lin bioengineering Co., Ltd.
Bacillus licheniformis (formal name used at school: Bacilluslicheniformis) is a kind of bacterium common in soil.Birds, particularly live in the birds (as Fringillidae) on ground and the feather of aquatic birds (as duck) and also can find in the feather at this bacterium, particularly chest and back.Bacillus licheniformis is a kind of gram-positive thermophilic bacteria.Its optimum growth temperature is approximately 30 DEG C, but also can survive at higher temperatures.The optimum temperature of enzyme secretion is 37 DEG C.It may exist with spore form, thus opposing rugged environment; Under good environment, then can grow state and exist.Bacillus licheniformis can decompose the poisonous and harmful substance in culturing pool, purifies water.Bacillus licheniformis has stronger protease, lipase, diastatic activity, promote the degraded of feed Middle nutrition element, make aquatic product animal more abundant to absorbing of feed, resistant activity material can be produced, and the biology with uniqueness takes oxygen mechanism of action by force, can suppress the growth and breeding of pathogenic bacteria.Bacillus licheniformis of the present invention is produced by the industrial Bioisystech Co., Ltd in Cangzhou.
VT bacterium is selected from the VT-1000 bacterium of commercially available Beijing Voto Sky & Land Biotech Co., Ltd.'s exploitation as the induction bacterium producing microbial population.Containing a large amount of bacterial strains and metabolite enzyme thereof in this microbial inoculum, by the effect of these viable bacterias and enzyme, crude protein, cellulose, lignin and the carbohydrate that can degrade in organic waste material on the one hand, produce the inorganic states nutrient and humus that easily utilize, thus improve the content of available nutrient, be conducive to absorbing of crop; On the other hand, these viable bacterias along with its breeding in rubbish heap can the growth of spoilage organisms always effectively, both alleviated generation and the environment bad-smell problem thereof of corrupt substance, had the problem causing soil-borne disease after these harmful bacterias can be suppressed to enter soil.
The spore non-helical of streptomyces cellulosae.Spore is spherical, oval, smooth surface, bacterium colony lemon look or yellow lemon yellow, and pigment permeates, and gas silk is light grey to lead.
Ammonia nitrogen degradation bacterium is chosen from Bi Wofeng biotechnology joint-stock company, and nitrobacteria can be divided into two large kinds: nitrosomonas and bacterium nitrobacter.Ammonium oxidation is become nitrite by nitrosomonas, and bacterium nitrobacter is then that nitrite-oxidizing is become nitrate.Nitrobacteria belongs to autotrophic bacterium (taking CO2 as carbon source), obtain the energy needed for growth and breeding, but its utilization rate is not high in above-mentioned oxidizing process, and thus accretion rate is very slow, is 2 times of the Conventional bacteria metabolism time, is approximately 8-16 hour.In addition, the activity of nitrobacteria and the speed of growth also can be subject to the impact of the aspects such as dissolved oxygen (DO), pH value, temperature and organic concentration.Generation cycle of nitrobacteria is long, the speed of growth is slow, does not have competitiveness completely compared with other heterotrophs, and in sewerage, nitrobacteria is often run off due to the impact of load or toxicant, or even dead.
Ammonia nitrogen degradation bacterium of the present invention is by the complete nitrobacteria screened from occurring in nature, adopt unique enzyme treatment technique formulated, nitrobacteria through screening and culturing has higher biologically active than conventional nitrobacteria, and the ability of stronger tolerance harmful toxic matter; Also with the addition of the nutrient of growth promoting effects simultaneously in product, greatly improve the growth and breeding speed of nitrobacteria, resist the impact of load or harmful toxic matter better, stablize the nitrification efficiency of garbage system.
Thermophilic Bacteria is also known as thermophilic bacteria, thermophilic microorganism, and it is the microorganism that a class lives in hot environment, and Thermophilic Bacteria is added in the rear hot environment of rubbish heap fermentation, contributes to some organic degradeds.Utilize Thermophilic Bacteria to carry out Anaerobic Treatment to waste water waste material, can reaction speed be improved, eliminate the pathogenic microorganism in sewage dirt.
During the fermentation, its resistant to elevated temperatures characteristic can be utilized, improve reaction temperature, augmenting response speed, the chance of warm type living contaminants in minimizing.
Wherein, the part by weight of the bacillus subtilis in described first composite bacteria liquid, bacillus licheniformis, VT bacterium is 1:1:0.5-1.Mixing composite bacteria liquid can make bacillus subtilis, bacillus licheniformis, VT bacterium mutually promote, and mutually restrict, thus reach the object of stimulus pool activity, ferment effect is good, due to the restriction of various thalline, the transition of single thalline can not be caused to spread unchecked.
Wherein, described second composite bacteria liquid comprise cellulose-degrading bacteria, ammonia nitrogen degradation bacterium, Thermophilic Bacteria part by weight be 1:1.5-2:2-2.5, the activity characteristic interdependence of various thalline and restriction, form the fermentation system of a simulated environment, ferment effect can be made stronger, kill the successful of pathogen.
Wherein, the compound bacteria content of described first composite bacteria liquid is 5-10%, and compound bacteria refers to the Mixed Microbes of bacillus subtilis, bacillus licheniformis and VT bacterium.The excessive concentration of bacterium liquid, then can cause waste, and can restrict the system of fermentation, affects fermentation rate.
Wherein, the compound bacteria content of described second composite bacteria liquid is 1-5%.Compound bacteria refers to that bacterium liquid comprises the Mixed Microbes of cellulose-degrading bacteria, ammonia nitrogen degradation bacterium and Thermophilic Bacteria.The excessive concentration of bacterium liquid, then can cause waste, and can restrict the system of fermentation, affects fermentation rate.
Wherein, the spray flux spraying the first composite bacteria liquid is 100-150 liters/min, continues spray 10 minutes, is conducive to the uniformly penetrating of bacterium liquid.
Wherein, the spray flux of spraying the second composite bacteria liquid is 80-130 liters/min, continues spray 5 minutes.Be conducive to the uniformly penetrating of bacterium liquid.
Wherein, the locality that described cellulose-degrading bacteria takes from rubbish source the rot stalk, trees, soil and cow dung one or more; Preserving number numbering 4.381, China General Microbiological culture presevation administrative center.
Concrete culture medium is: soluble starch 20.0g
KNO31.0g
K2HPO40.5g
MgSO4·7H2O0.5g
NaCl0.5g
FeSO40.01g
Agar 15.0g
Distilled water 1.0L
PH7.2-7.4, carries out enrichment culture to cellulose-degrading bacteria and is carrying out separation screening.
Beneficial effect of the present invention: what 1, the present invention adopted is that biological aerobic fermentation produces heat, thus utilize high temperature to reach to kill the object infecting pathogeny and the four pests, the ferment effect of the aerobic fermentation bacterium that tradition is single is not strong, easily lose activity, the present invention is by the microorganism compound use of several aerobic fermentation, sprays in rubbish heap, simulate natural environment, various biological bacteria synergy, mutual Developing restraint, rubbish heap provides nutritional labeling.
2, by transforming the packed structures of rubbish heap, by rubbish heap on aeration tower, being then conducive to the air circulation in rubbish, providing sufficient oxygen.Abandoned the operator scheme needing air blast in traditional aerobic fermentation, the pathogen in rubbish heap can take away by traditional air blast pattern, propagates in the environment of surrounding, and natural air circulation of the present invention then solves this technical problem.
3, most of biological bacteria that the present invention adopts is all conventional biological bacteria, and Costco Wholesale is low, and it is convenient to cultivate.The temperature of rubbish heap fermentation can up to 60-80 DEG C, quick heating, and kill pathogen rapid, treatment effect is good, and efficiency is high, has very strong market prospects, also plays great effect to environmental protection.
4, biological inoculum of the present invention adopts multiple bacterial classification to mix, and from biology angle, not only can go out natural ecological environment by real simulation, be conducive to the fermentation of biological bacteria, can also make to produce mutual inhibitory action between each biological bacteria, prevent single biological bacteria excessive multiplication, cause biohazard.
5, aerobic fermentation of the present invention, produces stink few, can be close to residential block and arrange, greatly alleviate the problem of land used anxiety, and can improve the efficiency of garbage disposal to a certain extent, solve refuse pollution problem as soon as possible.
6, the present invention can effectively killing mosquito, cockroach, mouse can common evil, is the effective measures preventing infectious disease to infect.
7, the garbage energy after fermentation is enough in fertilizer, recycles, high financial profit.
Detailed description of the invention
The invention will be further described with the following Examples.
embodiment 1
A kind of low cost kills the method that house refuse infects pathogeny, the aeration tower of several tapers is built in garbage pool, rubbish can be deposited in above aeration tower, the bottom in rubbish pond arranges several injector heads, first above rubbish heap spray containing 5% the first composite bacteria liquid 10 minutes, spray flux is 100 liters/min, described first composite bacteria liquid include bacillus subtilis, bacillus licheniformis, VT bacterium ratio be: 1:1:1; Then the second composite bacteria liquid 5 minutes containing 4% is sprayed by injector head to rubbish heap after 2 hours, spray flux is 80 liters/min, described second composite bacteria liquid comprises streptomyces cellulosae, ammonia nitrogen degradation bacterium, Thermophilic Bacteria, its ratio is 1:1.5:2.5, rubbish heap fermentation produces heat, and rubbish heap temperature is 65 DEG C.
embodiment 2
A kind of low cost kills the method that house refuse infects pathogeny, the aeration tower of several tapers is built in garbage pool, rubbish can be deposited in above aeration tower, the bottom in rubbish pond arranges several injector heads, first above rubbish heap spray containing 8% the first composite bacteria liquid 10 minutes, spray flux is 120 liters/min, described first composite bacteria liquid include bacillus subtilis, bacillus licheniformis, VT bacterium ratio be: 1:1:0.5; Then the second composite bacteria liquid 5 minutes containing 1% is sprayed by injector head to rubbish heap after 3 hours, spray flux is 120 liters/min, described second composite bacteria liquid comprises streptomyces cellulosae, ammonia nitrogen degradation bacterium, Thermophilic Bacteria, its ratio is 1:2:2, rubbish heap fermentation produces heat, and rubbish heap temperature is 73 DEG C.
embodiment 3
A kind of low cost kills the method that house refuse infects pathogeny, the aeration tower of several tapers is built in garbage pool, rubbish can be deposited in above aeration tower, the bottom in rubbish pond arranges several injector heads, first above rubbish heap spray containing 10% the first composite bacteria liquid 10 minutes, spray flux is 150 liters/min, described first composite bacteria liquid include bacillus subtilis, bacillus licheniformis, VT bacterium ratio be: 1:1:1; Then the second composite bacteria liquid 5 minutes containing 5% is sprayed by injector head to rubbish heap after 4 hours, spray flux is 80 liters/min, described second composite bacteria liquid comprises streptomyces cellulosae, ammonia nitrogen degradation bacterium, Thermophilic Bacteria, its ratio is 1:1.5:2, rubbish heap fermentation produces heat, and rubbish heap temperature is: 75 DEG C.
embodiment 4
A kind of low cost kills the method that house refuse infects pathogeny, the aeration tower of several tapers is built in garbage pool, rubbish can be deposited in above aeration tower, the bottom in rubbish pond arranges several injector heads, first above rubbish heap spray containing 5% the first composite bacteria liquid 10 minutes, spray flux is 150 liters/min, described first composite bacteria liquid include bacillus subtilis, bacillus licheniformis, VT bacterium ratio be: 1:1:1; Then the second composite bacteria liquid 5 minutes containing 3% is sprayed by injector head to rubbish heap after 2 hours, spray flux is 130 liters/min, described second composite bacteria liquid comprises streptomyces cellulosae, ammonia nitrogen degradation bacterium, Thermophilic Bacteria, its ratio is 1:2:2.5, rubbish heap fermentation produces heat, and rubbish heap temperature is 80 DEG C.
Finally should be noted that; above embodiment is only in order to illustrate technical scheme of the present invention; but not limiting the scope of the invention; although done to explain to the present invention with reference to preferred embodiment; those of ordinary skill in the art is to be understood that; can modify to technical scheme of the present invention or equivalent replacement, and not depart from essence and the scope of technical solution of the present invention.

Claims (8)

1. a low cost kills the method that house refuse infects pathogeny, it is characterized in that: the aeration tower building several tapers in garbage pool, sanitary fill is on aeration tower, the bottom of garbage pool arranges several injector heads, first above rubbish heap, spray contains the first composite bacteria liquid, then spray the second composite bacteria liquid by injector head to rubbish heap after 2-4 hour, rubbish heap fermentation produces heat, rubbish heap temperature 60-80 DEG C; Described first composite bacteria liquid includes bacillus subtilis, bacillus licheniformis and VT bacterium; Described second composite bacteria liquid comprises streptomyces cellulosae, ammonia nitrogen degradation bacterium and Thermophilic Bacteria.
2. a kind of low cost according to claim 1 kills the method that house refuse infects pathogeny, it is characterized in that: the part by weight of the bacillus subtilis in described first composite bacteria liquid, bacillus licheniformis, VT bacterium is 1:1:0.5-1.
3. a kind of low cost according to claim 1 is killed house refuse and is infected the method for pathogeny, it is characterized in that: described second composite bacteria liquid comprise cellulose-degrading bacteria, ammonia nitrogen degradation bacterium, Thermophilic Bacteria part by weight be 1:1.5-2:2-2.5.
4. a kind of low cost according to claim 1 kills the method that house refuse infects pathogeny, it is characterized in that: the compound bacteria content of described first composite bacteria liquid is 5-10%.
5. a kind of low cost according to claim 1 kills the method that house refuse infects pathogeny, it is characterized in that: the compound bacteria content of described second composite bacteria liquid is 1-5%.
6. a kind of low cost according to claim 1 kills the method that house refuse infects pathogeny, it is characterized in that: the spray flux spraying the first composite bacteria liquid is 100-150 liters/min, continues spray 10 minutes.
7. a kind of low cost according to claim 1 kills the method that house refuse infects pathogeny, it is characterized in that: the spray flux of spraying the second composite bacteria liquid is 80-130 liters/min, continues spray 5 minutes.
8. a kind of low cost according to claim 1 is killed house refuse and is infected the method for pathogeny, it is characterized in that: the locality that streptomyces cellulosae takes from rubbish source place rot stalk, trees, soil and cow dung one or more; The culture medium of described streptomyces cellulosae is:
Soluble starch 20.0g
KNO 31.0g
K 2HPO 40.5g
MgSO 4·7H 2O0.5g
NaCl0.5g
FeSO 40.01g
Agar 15.0g
Distilled water 1.0L
PH value 7.2-7.4, carries out enrichment culture to streptomyces cellulosae and carries out separation screening again.
CN201410434224.3A 2014-08-29 2014-08-29 A kind of low cost kills the method that house refuse infects pathogeny Active CN104307841B (en)

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CN108580517A (en) * 2018-04-24 2018-09-28 广州市凯卫莎环保科技有限公司 Quaternity waste disposal agent and the preparation method and application thereof

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JP3297641B2 (en) * 1998-05-06 2002-07-02 エステック株式会社 Garbage processing apparatus and kitchen garbage processing method
CN1370829A (en) * 2001-02-27 2002-09-25 广州天蔚生物技术有限公司 Bacterial spawn for treating domestic organic garbage
JP4697945B2 (en) * 2005-06-21 2011-06-08 好美 篠原 Deodorizer and composting method that does not generate odor
JP5249567B2 (en) * 2007-12-06 2013-07-31 スターエンジニアリング株式会社 Organic waste composting process
CN101993831A (en) * 2009-08-10 2011-03-30 北京农学院 Composite fungicide used for treating house refuse compost and application method thereof
CN102093968B (en) * 2010-11-29 2012-09-19 中国农业科学院农业资源与农业区划研究所 Compound microbial inoculum for deodorization
CN102327630B (en) * 2011-09-15 2013-08-28 湖南省微生物研究所 Microbial strain and deodorant for municipal landfill, and preparation and application method thereof
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