CN104306732B - A kind of golden dry measure used in former times soft capsule and preparation method thereof - Google Patents
A kind of golden dry measure used in former times soft capsule and preparation method thereof Download PDFInfo
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- CN104306732B CN104306732B CN201410405246.7A CN201410405246A CN104306732B CN 104306732 B CN104306732 B CN 104306732B CN 201410405246 A CN201410405246 A CN 201410405246A CN 104306732 B CN104306732 B CN 104306732B
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/898—Orchidaceae (Orchid family)
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
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- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/898—Orchidaceae (Orchid family)
- A61K36/8984—Dendrobium
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- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/15—Preparation or pretreatment of starting material involving mechanical treatment, e.g. chopping up, cutting or grinding
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- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/55—Liquid-liquid separation; Phase separation
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Abstract
The present invention discloses a kind of golden dry measure used in former times soft capsule, including by 1 1.5:Roxburgh anoectochilus terminal bud and dendrobium candidum of 23 weight than mixing.Preparation process is as follows：Mix in proportion after roxburgh anoectochilus terminal bud and dendrobium candidum, drying to constant weight is simultaneously crushed and composite powder is made；Composite powder adds distilled water immersion, is then placed in thermostat water bath, and filtering preserves and identical amount of distilled water is added after extract solution, and repeats said process and merge extract solution；After filtered through gauze impurity, high speed centrifugation takes its supernatant and concentrate is made in rotary evaporation concentration；Absolute ethyl alcohol is added in concentrate, alcohol precipitation after concentrate is obtained, ethanol and precipitation, alcohol analysis gained sediment are centrifuged thereafter；Ethanol and acetone washing are first added into alcohol analysis gained sediment, its sediment is taken, is put into drying box and is dried to constant weight after being evaporated on boiling water bath, golden dry measure used in former times polysaccharide compound is produced and golden dry measure used in former times soft capsule is made.The golden dry measure used in former times soft capsule that the present invention is extracted, not only improves drug effect, saves medicinal material, and dosage is accurate, instant.
Description
Technical field
The present invention relates to a kind of golden dry measure used in former times soft capsule and preparation method thereof, the especially formulated in combination of dendrobium candidum and roxburgh anoectochilus terminal bud
Technology.
Background technology
Roxburgh anoectochilus terminal bud is a kind of rare traditional Chinese medicine, have the title of the king of medicine, for a long time with all herbal medicine, among the people to treat
Hand-foot-and-mouth disease diabetes hypertension headstroke heart disease tracheitis pulmonary tuberculosis acute, chronic hepatitis whelk senile plaque expellings
Blackspot etc., has good therapeutic effect, dendrobium candidum is one of Chinese nine big celestial grass, to pharyngolaryngitis cataract cardiovascular disease glycosurias
Sick and gastrointestinal disease have significant curative effect, while can strengthen the antifatigue anti anoxias brain nourishing and eyesight improvings of immunity of organisms anti-aging.This
Two kinds of medicines press this patent prescription, and its drug effect is complementary, can Pinggan Yang support kidney yin, can promote the production of body fluid nourishing the stomach improving eyesight beauty treatment again.Due to passing
Usage of uniting, which is decocted, stewes, power-consuming not Portable belt of taking a lot of work, and because decocting method is different, medicinal material extracts active ingredients not exclusively, cause medicine
The material wasting of resources.
The content of the invention
In order to solve the problem of prior art is present, present invention aims at provide a kind of golden dry measure used in former times soft capsule and its preparation side
Method, the golden dry measure used in former times soft capsule extracted using the preparation method not only improves drug effect, saves medicinal material, and dosage is accurate, eats side
Just.
To achieve the above object, technical solution of the present invention is：
A kind of golden dry measure used in former times soft capsule, it is characterised in that：Including roxburgh anoectochilus terminal bud and dendrobium candidum, the roxburgh anoectochilus terminal bud and dendrobium candidum are pressed
1-1.5:2-3 weight is than mixing.
The preparation method of the golden dry measure used in former times soft capsule, its preparation process is as follows：
Step one, mix in proportion after roxburgh anoectochilus terminal bud and dendrobium candidum, 80-85 DEG C of drying is crushed, mistake to constant weight with pulverizer
30-50 mesh sieves, are made composite powder；
Step 2, every 100 grams of composite powders add 10-15 times of distilled water immersion 24-30H, are then placed in 90-100 DEG C of perseverance
1-2H in warm water bath, filtering preserves extract solution；Extract liquid slag adds identical amount of distilled water, and repeats said process 2-3 times,
Merge extract solution；
Step 3, the extract solution after merging, after 4-6 layers of filtered through gauze impurity, under 3000-5000r./min at a high speed from
Heart 16-18 minutes, takes its supernatant, and supernatant rotary evaporation is concentrated into the 1/3-1/4 of original volume, and concentrate is made；
Step 4, adds absolute ethyl alcohol in concentrate, obtains the concentrate of 70%-75% concentration, and in 4-6 DEG C of low temperature
24-28H alcohol precipitations are stood, ethanol and precipitation, alcohol analysis gained sediment is centrifuged under the conditions of 3000-5000r/.min thereafter；
Step 5, the 95%-98% ethanol washing that 2-2.5 times of volume is first added in gained sediment is analysed to alcohol, then again
2-2.5 times of vol acetone washing of sediment is added, its sediment is taken, is evaporated on boiling water bath after water content 15%-25%
It is put into drying box, 50-55 DEG C of drying produces golden dry measure used in former times polysaccharide compound to constant weight；
Step 6, golden dry measure used in former times soft capsule is made by the golden dry measure used in former times polysaccharide compound of above-mentioned gained through soft capsule standard technology.
A kind of foregoing golden dry measure used in former times soft capsule preparation method, each 0.5 gram of gold dry measure used in former times soft capsule, suitable 10 grams of crude drug.
The present invention uses water extraction and alcohol precipitation method to extract roxburgh anoectochilus terminal bud and dendrobium candidum active ingredient so that golden dry measure used in former times soft capsule is made, to gold
The active ingredient tools such as alkaloid, polysaccharide, steroidal, luxuriant and rich with fragrance class, Cardiac glycosides, amino acid, trace element in line lotus and dendrobium candidum
There is protective effect, it is to avoid cause to extract incomplete because water solubility is relatively low, cause herb resource to waste.Furthermore, roxburgh anoectochilus terminal bud and iron sheet
The stem of noble dendrobium both medicinal materials collaboration is used, and can improve drug effect, solves it and the biased harmful effect of property of medicine negative and positive is used alone.
Two medicines are private in tradition, alkaloid is dissociated composition increase, to GI irritation compared with
Greatly, stew to drink and occasionally have the unconformable performance of enteron aisle.The present invention by both roxburgh anoectochilus terminal bud and dendrobium candidum mix after, its alkaloid into
Divide because effect is inlayed by functional group, molecular structure model changes, the enhancing of lipophilic free radical activity, to GI irritation declines,
Its anti-inflammatory is antibacterial, and clearing heat and expelling damp function is significantly improved.The water extraction and alcohol precipitation method that the present invention is used causes polysaccharide extract rate to greatly improve,
Human body immune function can be greatly improved by taking this product, have significant curative effect to suppressing growth of tumour cell.And use water extract-alcohol precipitation
Method makes effective component extraction rate high, and medicinal material utilization rate is high, extracts polysaccharide composite microbic activity height, and clinical efficacy is good.
Both roxburgh anoectochilus terminal bud and dendrobium candidum are the food materials of medicine-food two-purpose, and soft capsule is made in its active ingredient, can accomplish fixed
Amount is accurate, and easily to carry, instant edible.
Embodiment
In order that technical problems, technical solutions and advantages to be solved are clearer, clear, lead to below
Crossing embodiment, the present invention will be described in further detail.It should be appreciated that specific embodiment described herein is only to explain
The present invention, is not intended to limit the present invention.
Embodiment one
A kind of golden dry measure used in former times soft capsule of the present invention, including roxburgh anoectochilus terminal bud and dendrobium candidum, the roxburgh anoectochilus terminal bud and dendrobium candidum are by weight
Than 1：2 are mixed into 500 grams.
Above-mentioned golden dry measure used in former times soft capsule uses water extraction and alcohol precipitation method, and its preparation process is as follows：
Step one, mix after roxburgh anoectochilus terminal bud and dendrobium candidum, in 80 DEG C of drying to 458 grams of constant weight, use according to the above ratio
FW800 types high speed disintegrator is crushed, and crosses 40 mesh sieves, and composite powder is made；
Step 2, every 100 grams of composite powders are placed in beaker, plus 10 times of distilled water, i.e. 1000ml distilled water immersion
24H, is then placed in 1.5H in 95 DEG C of thermostat water baths, and filtering preserves extract solution；Extract liquid slag adds identical amount of distilled water, and
Repeat said process 2-3 times, merge extract solution；
Step 3, the extract solution after merging, after 4 layers of filtered through gauze impurity, 16 points of high speed centrifugation under 3000r./min
Clock, takes its supernatant, and supernatant rotary evaporation is concentrated into 1/3 volume ratio of original volume, and concentrate is made；
Step 4, adds absolute ethyl alcohol in concentrate, obtains the concentrate of 72% concentration, and in 4 DEG C of stand at low temperature 24H
Alcohol precipitation, centrifuges thereafter ethanol and precipitation, alcohol analysis gained sediment under the conditions of 3000r/.min；
Step 5,95% ethanol that 2 times of volumes are added in gained sediment is analysed to alcohol, 2 times of bodies of sediment are then added
Long-pending acetone is washed respectively, takes its sediment, and h water content 15% is evaporated on boiling water bath (HH-S6) and is put into electrically heated drying cabinet
(DZF6020) in, 50 DEG C of drying produce golden dry measure used in former times polysaccharide compound to 25.63 grams of constant weights；
Step 6, golden dry measure used in former times soft capsule is made by the golden dry measure used in former times polysaccharide compound of above-mentioned gained through soft capsule standard technology.Each
Golden 0.5 gram of dry measure used in former times soft capsule, suitable 10 grams of crude drug.
Embodiment two
A kind of golden dry measure used in former times soft capsule of the present invention, including roxburgh anoectochilus terminal bud and dendrobium candidum, the roxburgh anoectochilus terminal bud and dendrobium candidum are by weight
Than 1.2；2.6 are mixed into 500 grams.
The above-mentioned above-mentioned golden dry measure used in former times soft capsule of golden dry measure used in former times soft capsule uses water extraction and alcohol precipitation method, its preparation process is as follows：
Step one, mix according to the above ratio after roxburgh anoectochilus terminal bud and dendrobium candidum, in 82 DEG C of drying to 456 constant weights, use FW800 types
High speed disintegrator is crushed, and crosses 50 mesh sieves, and composite powder is made；
Step 2, every 100 grams of composite powders are placed in beaker, plus 12 times of distilled water immersion 25H, are then placed in 90 DEG C of perseverances
1H in warm water bath, filtering preserves extract solution；Extract liquid slag adds identical amount of distilled water, and repeats said process 2-3 times,
Merge extract solution；
Step 3, the extract solution after merging, after 5 layers of filtered through gauze impurity, 17 points of high speed centrifugation under 3500r./min
Clock, takes its supernatant, and supernatant rotary evaporation is concentrated into 7/12 volume ratio of original volume, and concentrate is made；
Step 4, adds absolute ethyl alcohol in concentrate, obtains the concentrate of 75% concentration, and in 5 DEG C of stand at low temperature 26H
Alcohol precipitation, centrifuges thereafter ethanol and precipitation, alcohol analysis gained sediment under the conditions of 4500r/.min；
Step 5,96% ethanol that 2.25 times of volumes are added in gained sediment is analysed to alcohol, sediment is then added
The acetone of 2.25 times of volumes is washed respectively, takes its sediment, and water content 18% is evaporated on boiling water bath (HH-S6 models) and is put into
Electrically heated drying cabinet (DZF6020)) in, 55 DEG C of drying produce golden dry measure used in former times polysaccharide compound to 25.36 grams of constant weight；
Step 6, golden dry measure used in former times soft capsule is made by the golden dry measure used in former times polysaccharide compound of above-mentioned gained through soft capsule standard technology.Each
Golden 0.5 gram of dry measure used in former times soft capsule, suitable 10 grams of crude drug.
Embodiment three
A kind of golden dry measure used in former times soft capsule of the present invention, including roxburgh anoectochilus terminal bud and dendrobium candidum, the roxburgh anoectochilus terminal bud and dendrobium candidum are by weight
Than 1.5：3 are mixed into 500 grams.
The above-mentioned above-mentioned golden dry measure used in former times soft capsule of golden dry measure used in former times soft capsule uses water extraction and alcohol precipitation method, and its preparation process is as follows：
Step one, mix according to the above ratio after roxburgh anoectochilus terminal bud and dendrobium candidum, in 85 DEG C of drying to 455 grams of constant weights, use FW800
Type high speed disintegrator is crushed, and crosses 30 mesh sieves, and composite powder is made；
Step 2, every 100 grams of composite powders are placed in beaker, plus 15 times of distilled water immersion 30H, are then placed in 100 DEG C
2H in thermostat water bath, filtering preserves extract solution；Extract liquid slag adds identical amount of distilled water, and repeats said process 2-3 times,
Merge extract solution；
Step 3, the extract solution after merging, after 6 layers of filtered through gauze impurity, 18 points of high speed centrifugation under 5000r./min
Clock, takes its supernatant, and supernatant rotary evaporation is concentrated into 1/4 volume ratio of original volume, and concentrate is made；
Step 4, adds absolute ethyl alcohol in concentrate, obtains 70% concentrate, and in 6 DEG C of stand at low temperature 28H alcohol
It is heavy, ethanol and precipitation, alcohol analysis gained sediment are centrifuged under the conditions of 5000r/.min thereafter；
Step 5,98% ethanol that 2.5 times of volumes are added in gained sediment is analysed to alcohol, sediment 2.5 is then added
The acetone of times volume is washed respectively, takes its sediment, and water content 25% is evaporated on boiling water bath (model HH-S6) and is put into electric heating
In drying box (DZF6020), 53 DEG C of drying produce golden dry measure used in former times polysaccharide compound to 25.12 grams；
Step 6, golden dry measure used in former times soft capsule is made by the golden dry measure used in former times polysaccharide compound of above-mentioned gained through soft capsule standard technology.Each
Golden 0.5 gram of dry measure used in former times soft capsule, suitable 10 grams of crude drug.
The preferred embodiments of the present invention have shown and described in described above, as previously described, it should be understood that not office of the invention
Be limited to form disclosed herein, be not to be taken as the exclusion to other embodiment, and available for various other combinations, modification and
Environment, and can be changed in invention contemplated scope described herein by the technology or knowledge of above-mentioned teaching or association area
It is dynamic., then all should be appended by the present invention and the change and change that those skilled in the art are carried out do not depart from the spirit and scope of the present invention
In scope of the claims.
Claims (2)
1. a kind of golden dry measure used in former times soft capsule, it is characterised in that：The golden dry measure used in former times soft capsule includes roxburgh anoectochilus terminal bud and dendrobium candidum, the roxburgh anoectochilus terminal bud
1-1.5 is pressed with dendrobium candidum：2-3 weight is than mixing；The preparation process of the golden dry measure used in former times soft capsule is as follows：
Step one, mix in proportion after roxburgh anoectochilus terminal bud and dendrobium candidum, 80-85 DEG C of drying is crushed, mistake to constant weight with pulverizer
30-50 mesh sieves, are made composite powder；
Step 2, every 100 grams of composite powders add 10-15 times of distilled water immersion 24-30h, are then placed in 90-100 DEG C of constant temperature
1-2h in water-bath, filtering preserves extract solution；Extract liquid slag adds identical amount of distilled water, and repeats said process 2-3 times,
Merge extract solution；
Step 3, the extract solution after merging, after 4-6 layers of filtered through gauze impurity, the high speed centrifugation under 3000-5000r./min
16-18 minutes, its supernatant is taken, supernatant rotary evaporation is concentrated into the 1/3-1/4 of original volume, concentrate is made；
Step 4, adds absolute ethyl alcohol in concentrate, obtains the concentrate of 70%-75% concentration, and quiet in 4-6 DEG C of low temperature
24-28h alcohol precipitations are put, ethanol and precipitation, alcohol analysis gained sediment is centrifuged under the conditions of 3000-5000r/.min thereafter；
Step 5, the 95%-98% ethanol washing that 2-2.5 times of volume is first added in gained sediment is analysed to alcohol, is then added again
Enter 2-2.5 times of vol acetone washing of sediment, take its sediment, be evaporated on boiling water bath after water content 15%-25%
It is put into drying box, 50-55 DEG C of drying produces golden dry measure used in former times polysaccharide compound to constant weight；
Step 6, golden dry measure used in former times soft capsule is made by the golden dry measure used in former times polysaccharide compound of above-mentioned gained through soft capsule standard technology.
2. a kind of golden dry measure used in former times soft capsule as claimed in claim 1, is characterised by：Each 0.5 gram of gold dry measure used in former times soft capsule, suitable crude drug 10
Gram.
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| CN104666934B (en) * | 2015-03-11 | 2018-05-01 | 劲牌生物医药有限公司 | Cordyceps sinensis compound buccal lozenge and preparation method thereof |
| CN105192203A (en) * | 2015-10-16 | 2015-12-30 | 闽侯县农心农业科技示范园 | Formula of healthy tea containing moringa oleifera lam, anoectochilus roxburghii and dendrobium as well as making technology and application of healthy tea |
| CN106389870A (en) * | 2016-08-28 | 2017-02-15 | 广西吉光生态科技有限公司 | Anoectochilus roxburghii pill and preparation method thereof |
| CN115154553A (en) * | 2022-07-21 | 2022-10-11 | 福建稻鲜生文旅集团有限公司 | Anoectochilus roxburghii compound blood glucose reducing oral liquid, circulating extraction double-effect concentration preparation device and preparation method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103099285A (en) * | 2013-01-18 | 2013-05-15 | 六安同济生生物科技有限公司 | Preparation method of dendrobium huoshanense composite fresh juice and oral liquid thereof |
| CN103893567A (en) * | 2014-04-21 | 2014-07-02 | 广西浙商投资有限公司 | Method for preparing and processing dendrobium nobile capsule |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103099285A (en) * | 2013-01-18 | 2013-05-15 | 六安同济生生物科技有限公司 | Preparation method of dendrobium huoshanense composite fresh juice and oral liquid thereof |
| CN103893567A (en) * | 2014-04-21 | 2014-07-02 | 广西浙商投资有限公司 | Method for preparing and processing dendrobium nobile capsule |
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