CN104306732A - A roxburgh anoectochilus terminal bud-dendrobium officinale soft capsule and a preparing method thereof - Google Patents
A roxburgh anoectochilus terminal bud-dendrobium officinale soft capsule and a preparing method thereof Download PDFInfo
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- CN104306732A CN104306732A CN201410405246.7A CN201410405246A CN104306732A CN 104306732 A CN104306732 A CN 104306732A CN 201410405246 A CN201410405246 A CN 201410405246A CN 104306732 A CN104306732 A CN 104306732A
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/898—Orchidaceae (Orchid family)
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
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- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/898—Orchidaceae (Orchid family)
- A61K36/8984—Dendrobium
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- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/15—Preparation or pretreatment of starting material involving mechanical treatment, e.g. chopping up, cutting or grinding
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- A—HUMAN NECESSITIES
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- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/55—Liquid-liquid separation; Phase separation
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Abstract
A roxburgh anoectochilus terminal bud-dendrobium officinale soft capsule is disclosed. The soft capsule comprises roxburgh anoectochilus terminal bud and dendrobium officinale which are mixed according to a weight ratio of 1-1.5:2-3. The soft capsule is prepared by following steps of: mixing the roxburgh anoectochilus terminal bud and the dendrobium officinale according to the ratio, drying until the weight is constant, smashing to obtain composite medicine powder, adding distilled water into the composite medicine powder, dipping, putting into a thermostat water bath, filtering, keeping an extracting solution, adding distilled water with the same amount, repeating the above steps, combining the extracting solutions, filtering with gauze to remove impurities, centrifuging at a high speed, subjecting the liquid supernatant to rotary evaporation and concentration to obtain a concentrate liquid, adding absolute ethyl alcohol into the concentrate liquid to obtain a concentrate solution, subjecting the concentrate solution to alcohol precipitation, performing centrifugal separation to separate the ethyl alcohol and a precipitate, performing alcohol precipitation to obtain the precipitate, adding ethyl alcohol and acetone into the precipitate to wash the precipitate, subjecting the precipitate to evaporation in a boiling water bath, putting the precipitate into a drying oven, drying until the weight is constant to obtain a roxburgh anoectochilus terminal bud-dendrobium officinale polysaccharide composite, and preparing the soft capsule. The soft capsule is enhanced in medicine effects, saved in medicine materials, accurate in dosage and convenient to eat.
Description
Technical field
The present invention relates to a kind of golden dry measure used in former times soft capsule and preparation method thereof, especially the formulated in combination technology of Herba Dendrobii and Herba Anoectochili roxburghii.
Background technology
Herba Anoectochili roxburghii is a kind of rare Chinese medicine, have the title of the king of medicine, for a long time all with all herbal medicine, among the people in order to treat hand-foot-mouth disease. diabetes. hypertension. apoplexy. heart disease. tracheitis. pulmonary tuberculosis. acute, chronic hepatitis. comedo. senile plaque. black speck etc., all there is good therapeutic effect, Herba Dendrobii is one of China's nine large Herba mesonae chinensis, to laryngopharyngeal diseases cataract. cardiovascular diseases. and diabetes. and gastrointestinal disease has significant curative effect, can resist aging strenuously by enhancing human body immunity simultaneously. and resisting fatigue. anti-hypoxia brain nourishing and eyesight improving.This patent prescription pressed by these two kinds of medicines, and its drug effect is complementary, can support kidney yin, nourishing the stomach improving eyesight skin care of promoting the production of body fluid again by suppressing the hyperactive liver sun.Stew because conventional use decocts, the expense electricity of taking a lot of work is Portable belt not, and because decocting method is different, medical material extracts active ingredients is incomplete, causes herb resource to waste.
Summary of the invention
In order to solve prior art Problems existing, the object of the invention is to provide a kind of golden dry measure used in former times soft capsule and preparation method thereof, and the golden dry measure used in former times soft capsule adopting this preparation method to extract, not only improves drug effect, saves medical material, and dosage is accurate, instant.
For achieving the above object, technical solution of the present invention is:
A kind of golden dry measure used in former times soft capsule, is characterized in that: comprise Herba Anoectochili roxburghii and Herba Dendrobii, and described Herba Anoectochili roxburghii and Herba Dendrobii press the weight ratio mixing of 1-1.5:2-3.
The preparation method of described golden dry measure used in former times soft capsule, its preparation process is as follows:
Step one, after being mixed in proportion Herba Anoectochili roxburghii and Herba Dendrobii, drying to constant weight for 80-85 DEG C, pulverizes with pulverizer, cross 30-50 mesh sieve, obtained compound medicated powder;
Step 2, every 100 grams of compound medicated powder add 10-15 distilled water immersion 24-30H doubly, then put into 90-100 DEG C of thermostat water bath 1-2H, filter and preserve extracting solution; Extract melt cinder and add the identical distillation water yield, and repeat said process 2-3 time, merge extractive liquid;
Step 3, the extracting solution after merging, after 4-6 layer filtered through gauze impurity, high speed centrifugation 16-18 minute under 3000-5000r./min, gets its supernatant, supernatant rotary evaporation is concentrated into the 1/3-1/4 of original volume, obtained concentrated solution;
Step 4, adds dehydrated alcohol in concentrated solution, obtains the concentrated solution of 70%-75% concentration, and at 4-6 DEG C of stand at low temperature 24-28H precipitate with ethanol, centrifugalize ethanol and precipitation under 3000-5000r/.min condition thereafter, gained precipitate analysed by alcohol;
Step 5, the 95%-98% washing with alcohol first adding 2-2.5 times of volume in gained precipitate is analysed to alcohol, then add precipitate 2-2.5 times of vol acetone washing again, get its precipitate, drying baker is put into after boiling water bath is evaporated to water content 15%-25%, dry to constant weight, obtain golden dry measure used in former times polysaccharides compound for 50-55 DEG C;
Step 6, obtains golden dry measure used in former times soft capsule by the golden dry measure used in former times polysaccharides compound of above-mentioned gained through soft capsule standard technology.
The preparation method of aforesaid a kind of golden dry measure used in former times soft capsule, each golden dry measure used in former times soft capsule 0.5 gram, suitable crude drug 10 grams.
The present invention adopts decoction and alcohol sedimentation technique extraction Herba Anoectochili roxburghii and Herba Dendrobii effective ingredient to make golden dry measure used in former times soft capsule; to effective ingredient such as the alkaloid in Herba Anoectochili roxburghii and Herba Dendrobii, polysaccharide, steroidal, luxuriant and rich with fragrance class, Cardiac glycosides, aminoacid, trace element, there is protective effect; avoid causing because water-soluble degree is lower extracting not exclusively, cause herb resource to waste.Moreover Herba Anoectochili roxburghii and the collaborative use of these two kinds of medical materials of Herba Dendrobii, can improve drug effect, solve it and be used alone the biased harmful effect of property of medicine negative and positive.
In tradition, two medicines are private, and the composition that dissociates of alkaloid in Herba Anoectochili roxburghii or Herba Dendrobii is increased, comparatively large to GI irritation, stew and drink the even performance having intestinal discomfort to answer.After both Herba Anoectochili roxburghii and Herba Dendrobii mix by the present invention, its alkaloid component inlays effect because of functional group, and molecular structure model changes, and lipophilic free radical activity strengthens, and to GI irritation declines, its antiinflammatory is antibacterial, and clearing heat and expelling damp function significantly improves.The decoction and alcohol sedimentation technique that the present invention adopts makes polysaccharide extract rate significantly improve, and taking this product greatly can improve human body immune function, has significant curative effect to inhibition tumor cell growth.And adopt decoction and alcohol sedimentation technique to make effective component extraction rate high, medical material utilization rate is high, to extract polysaccharide composite microbic activity high, clinical efficacy is good.
Both Herba Anoectochili roxburghii and Herba Dendrobii are the food materials of medicine-food two-purpose, and its effective ingredient makes soft capsule, can accomplish quantitatively accurately, and easily to carry, instant edible.
Detailed description of the invention
In order to make technical problem to be solved by this invention, technical scheme and beneficial effect clearly, understand, by the following examples the present invention is further elaborated.Should be appreciated that specific embodiment described herein only in order to explain the present invention, be not intended to limit the present invention.
Embodiment one
A kind of golden dry measure used in former times soft capsule of the present invention, comprises Herba Anoectochili roxburghii and Herba Dendrobii, and described Herba Anoectochili roxburghii and Herba Dendrobii are mixed into 500 grams by weight 1:2.
Above-mentioned golden dry measure used in former times soft capsule adopts decoction and alcohol sedimentation technique, and its preparation process is as follows:
Step one, after mixing Herba Anoectochili roxburghii and Herba Dendrobii according to the above ratio, dries the constant weight to 458 grams at 80 DEG C, pulverizes with FW800 type high speed disintegrator, cross 40 mesh sieves, obtained compound medicated powder;
Step 2, every 100 grams of compound medicated powder are placed in beaker, add the distilled water of 10 times, i.e. the distilled water immersion 24H of 1000ml, then put into 95 DEG C of thermostat water bath 1.5H, filter and preserve extracting solution; Extract melt cinder and add the identical distillation water yield, and repeat said process 2-3 time, merge extractive liquid;
Step 3, the extracting solution after merging, after 4 layers of filtered through gauze impurity, under 3000r./min, high speed centrifugation 16 minutes, gets its supernatant, and supernatant rotary evaporation is concentrated into 1/3 volume ratio of original volume, obtained concentrated solution;
Step 4, adds dehydrated alcohol in concentrated solution, obtains the concentrated solution of 72% concentration, and at 4 DEG C of stand at low temperature 24H precipitate with ethanol, centrifugalize ethanol and precipitation under 3000r/.min condition thereafter, gained precipitate analysed by alcohol;
Step 5,95% ethanol adding 2 times of volumes is analysed in gained precipitate to alcohol, the acetone then adding precipitate 2 times of volumes again washs respectively, get its precipitate, electrically heated drying cabinet (DZF6020) is put in boiling water bath (HH-S6) upper evaporation h water content 15%, dry to 25.63 grams of constant weights, obtain golden dry measure used in former times polysaccharides compound for 50 DEG C;
Step 6, obtains golden dry measure used in former times soft capsule by the golden dry measure used in former times polysaccharides compound of above-mentioned gained through soft capsule standard technology.Each golden dry measure used in former times soft capsule 0.5 gram, suitable crude drug 10 grams.
Embodiment two
A kind of golden dry measure used in former times soft capsule of the present invention, comprises Herba Anoectochili roxburghii and Herba Dendrobii, and described Herba Anoectochili roxburghii and Herba Dendrobii are by weight 1.2; 2.6 are mixed into 500 grams.
The above-mentioned golden dry measure used in former times soft capsule of above-mentioned golden dry measure used in former times soft capsule adopts decoction and alcohol sedimentation technique, its preparation process is as follows:
Step one, after mixing Herba Anoectochili roxburghii and Herba Dendrobii according to the above ratio, dries to 456 constant weights at 82 DEG C, pulverizes with FW800 type high speed disintegrator, cross 50 mesh sieves, obtained compound medicated powder;
Step 2, every 100 grams of compound medicated powder are placed in beaker, add the distilled water immersion 25H of 12 times, then put into 90 DEG C of thermostat water bath 1H, filter and preserve extracting solution; Extract melt cinder and add the identical distillation water yield, and repeat said process 2-3 time, merge extractive liquid;
Step 3, the extracting solution after merging, after 5 layers of filtered through gauze impurity, under 3500r./min, high speed centrifugation 17 minutes, gets its supernatant, and supernatant rotary evaporation is concentrated into 7/12 volume ratio of original volume, obtained concentrated solution;
Step 4, adds dehydrated alcohol in concentrated solution, obtains the concentrated solution of 75% concentration, and at 5 DEG C of stand at low temperature 26H precipitate with ethanol, centrifugalize ethanol and precipitation under 4500r/.min condition thereafter, gained precipitate analysed by alcohol;
Step 5,96% ethanol adding 2.25 times of volumes is analysed in gained precipitate to alcohol, the acetone then adding precipitate 2.25 times of volumes again washs respectively, get its precipitate, electrically heated drying cabinet (DZF6020) is put into) in boiling water bath (HH-S6 model) upper evaporation water content 18%, dry to constant weight 25.36 grams, obtain golden dry measure used in former times polysaccharides compound for 55 DEG C;
Step 6, obtains golden dry measure used in former times soft capsule by the golden dry measure used in former times polysaccharides compound of above-mentioned gained through soft capsule standard technology.Each golden dry measure used in former times soft capsule 0.5 gram, suitable crude drug 10 grams.
Embodiment three
A kind of golden dry measure used in former times soft capsule of the present invention, comprises Herba Anoectochili roxburghii and Herba Dendrobii, and described Herba Anoectochili roxburghii and Herba Dendrobii are mixed into 500 grams by weight 1.5:3.
The above-mentioned golden dry measure used in former times soft capsule of above-mentioned golden dry measure used in former times soft capsule adopts decoction and alcohol sedimentation technique, and its preparation process is as follows:
Step one, after mixing Herba Anoectochili roxburghii and Herba Dendrobii according to the above ratio, dries to 455 grams of constant weights at 85 DEG C, pulverizes with FW800 type high speed disintegrator, cross 30 mesh sieves, obtained compound medicated powder;
Step 2, every 100 grams of compound medicated powder are placed in beaker, add the distilled water immersion 30H of 15 times, then put into 100 DEG C of thermostat water bath 2H, filter and preserve extracting solution; Extract melt cinder and add the identical distillation water yield, and repeat said process 2-3 time, merge extractive liquid;
Step 3, the extracting solution after merging, after 6 layers of filtered through gauze impurity, under 5000r./min, high speed centrifugation 18 minutes, gets its supernatant, and supernatant rotary evaporation is concentrated into 1/4 volume ratio of original volume, obtained concentrated solution;
Step 4, adds dehydrated alcohol in concentrated solution, obtains the concentrated solution of 70%, and at 6 DEG C of stand at low temperature 28H precipitate with ethanol, centrifugalize ethanol and precipitation under 5000r/.min condition thereafter, gained precipitate analysed by alcohol;
Step 5,98% ethanol adding 2.5 times of volumes is analysed in gained precipitate to alcohol, the acetone then adding precipitate 2.5 times of volumes again washs respectively, get its precipitate, electrically heated drying cabinet (DZF6020) is put in boiling water bath (model HH-S6) upper evaporation water content 25%, dry to 25.12 grams, obtain golden dry measure used in former times polysaccharides compound for 53 DEG C;
Step 6, obtains golden dry measure used in former times soft capsule by the golden dry measure used in former times polysaccharides compound of above-mentioned gained through soft capsule standard technology.Each golden dry measure used in former times soft capsule 0.5 gram, suitable crude drug 10 grams.
Above-mentioned explanation illustrate and describes the preferred embodiments of the present invention, as previously mentioned, be to be understood that the present invention is not limited to the form disclosed by this paper, should not regard the eliminating to other embodiments as, and can be used for other combinations various, amendment and environment, and can in invention contemplated scope described herein, changed by the technology of above-mentioned instruction or association area or knowledge.And the change that those skilled in the art carry out and change do not depart from the spirit and scope of the present invention, then all should in the protection domain of claims of the present invention.
Claims (3)
1. a golden dry measure used in former times soft capsule, is characterized in that: comprise Herba Anoectochili roxburghii and Herba Dendrobii, and described Herba Anoectochili roxburghii and Herba Dendrobii press the weight ratio mixing of 1-1.5:2-3.
2. a preparation method for golden dry measure used in former times soft capsule, is characterised in that: its preparation process is as follows:
Step one, after being mixed in proportion Herba Anoectochili roxburghii and Herba Dendrobii, drying to constant weight for 80-85 DEG C, pulverizes with pulverizer, cross 30-50 mesh sieve, obtained compound medicated powder;
Step 2, every 100 grams of compound medicated powder add 10-15 distilled water immersion 24-30H doubly, then put into 90-100 DEG C of thermostat water bath 1-2H, filter and preserve extracting solution; Extract melt cinder and add the identical distillation water yield, and repeat said process 2-3 time, merge extractive liquid;
Step 3, the extracting solution after merging, after 4-6 layer filtered through gauze impurity, high speed centrifugation 16-18 minute under 3000-5000r./min, gets its supernatant, supernatant rotary evaporation is concentrated into the 1/3-1/4 of original volume, obtained concentrated solution;
Step 4, adds dehydrated alcohol in concentrated solution, obtains the concentrated solution of 70%-75% concentration, and at 4-6 DEG C of stand at low temperature 24-28H precipitate with ethanol, centrifugalize ethanol and precipitation under 3000-5000r/.min condition thereafter, gained precipitate analysed by alcohol;
Step 5, the 95%-98% washing with alcohol first adding 2-2.5 times of volume in gained precipitate is analysed to alcohol, then add precipitate 2-2.5 times of vol acetone washing again, get its precipitate, drying baker is put into after boiling water bath is evaporated to water content 15%-25%, dry to constant weight, obtain golden dry measure used in former times polysaccharides compound for 50-55 DEG C;
Step 6, obtains golden dry measure used in former times soft capsule by the golden dry measure used in former times polysaccharides compound of above-mentioned gained through soft capsule standard technology.
3. the preparation method of a kind of golden dry measure used in former times soft capsule as claimed in claim 2, is characterised in that: each golden dry measure used in former times soft capsule 0.5 gram, suitable crude drug 10 grams.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104666934A (en) * | 2015-03-11 | 2015-06-03 | 劲牌生物医药有限公司 | Cordyceps sinensis compound lozenge and preparation method thereof |
| CN105192203A (en) * | 2015-10-16 | 2015-12-30 | 闽侯县农心农业科技示范园 | Formula of healthy tea containing moringa oleifera lam, anoectochilus roxburghii and dendrobium as well as making technology and application of healthy tea |
| CN106389870A (en) * | 2016-08-28 | 2017-02-15 | 广西吉光生态科技有限公司 | Anoectochilus roxburghii pill and preparation method thereof |
| CN115154553A (en) * | 2022-07-21 | 2022-10-11 | 福建稻鲜生文旅集团有限公司 | Anoectochilus roxburghii compound blood glucose reducing oral liquid, circulating extraction double-effect concentration preparation device and preparation method thereof |
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| CN103099285A (en) * | 2013-01-18 | 2013-05-15 | 六安同济生生物科技有限公司 | Preparation method of dendrobium huoshanense composite fresh juice and oral liquid thereof |
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| CN103099285A (en) * | 2013-01-18 | 2013-05-15 | 六安同济生生物科技有限公司 | Preparation method of dendrobium huoshanense composite fresh juice and oral liquid thereof |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104666934A (en) * | 2015-03-11 | 2015-06-03 | 劲牌生物医药有限公司 | Cordyceps sinensis compound lozenge and preparation method thereof |
| CN105192203A (en) * | 2015-10-16 | 2015-12-30 | 闽侯县农心农业科技示范园 | Formula of healthy tea containing moringa oleifera lam, anoectochilus roxburghii and dendrobium as well as making technology and application of healthy tea |
| CN106389870A (en) * | 2016-08-28 | 2017-02-15 | 广西吉光生态科技有限公司 | Anoectochilus roxburghii pill and preparation method thereof |
| CN115154553A (en) * | 2022-07-21 | 2022-10-11 | 福建稻鲜生文旅集团有限公司 | Anoectochilus roxburghii compound blood glucose reducing oral liquid, circulating extraction double-effect concentration preparation device and preparation method thereof |
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