CN104303845B - A kind of method for considering culture ganoderma lucidum to be worth doing using the twigs of the chaste tree - Google Patents
A kind of method for considering culture ganoderma lucidum to be worth doing using the twigs of the chaste tree Download PDFInfo
- Publication number
- CN104303845B CN104303845B CN201410611995.5A CN201410611995A CN104303845B CN 104303845 B CN104303845 B CN 104303845B CN 201410611995 A CN201410611995 A CN 201410611995A CN 104303845 B CN104303845 B CN 104303845B
- Authority
- CN
- China
- Prior art keywords
- twigs
- chaste tree
- ganoderma lucidum
- culture medium
- culture
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 235000001667 Vitex agnus castus Nutrition 0.000 title claims abstract description 95
- 240000008397 Ganoderma lucidum Species 0.000 title claims abstract description 43
- 235000001637 Ganoderma lucidum Nutrition 0.000 title claims abstract description 42
- 238000000034 method Methods 0.000 title claims abstract description 18
- 244000063464 Vitex agnus-castus Species 0.000 title description 2
- 241000532412 Vitex Species 0.000 claims abstract description 94
- 239000007788 liquid Substances 0.000 claims abstract description 38
- 239000001963 growth medium Substances 0.000 claims abstract description 36
- ODINCKMPIJJUCX-UHFFFAOYSA-N Calcium oxide Chemical compound [Ca]=O ODINCKMPIJJUCX-UHFFFAOYSA-N 0.000 claims abstract description 30
- 235000013339 cereals Nutrition 0.000 claims abstract description 26
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 19
- 235000015099 wheat brans Nutrition 0.000 claims abstract description 17
- 229920000742 Cotton Polymers 0.000 claims abstract description 16
- 238000002360 preparation method Methods 0.000 claims abstract description 16
- 239000000292 calcium oxide Substances 0.000 claims abstract description 15
- 235000012255 calcium oxide Nutrition 0.000 claims abstract description 15
- 239000000758 substrate Substances 0.000 claims abstract description 10
- 230000006698 induction Effects 0.000 claims abstract description 7
- 239000000203 mixture Substances 0.000 claims abstract description 5
- 239000002361 compost Substances 0.000 claims description 12
- 239000002054 inoculum Substances 0.000 claims description 10
- 239000000463 material Substances 0.000 claims description 10
- 241000209140 Triticum Species 0.000 claims description 9
- 235000021307 Triticum Nutrition 0.000 claims description 9
- 230000001954 sterilising effect Effects 0.000 claims description 9
- 241000894006 Bacteria Species 0.000 claims description 8
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 8
- 244000061456 Solanum tuberosum Species 0.000 claims description 8
- 235000002595 Solanum tuberosum Nutrition 0.000 claims description 8
- 239000008103 glucose Substances 0.000 claims description 8
- 239000000843 powder Substances 0.000 claims description 8
- 229920002134 Carboxymethyl cellulose Polymers 0.000 claims description 7
- 239000007836 KH2PO4 Substances 0.000 claims description 7
- 235000010948 carboxy methyl cellulose Nutrition 0.000 claims description 7
- 238000003306 harvesting Methods 0.000 claims description 7
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 7
- CSNNHWWHGAXBCP-UHFFFAOYSA-L magnesium sulphate Substances [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 7
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 7
- 239000007921 spray Substances 0.000 claims description 6
- 235000001674 Agaricus brunnescens Nutrition 0.000 claims description 5
- 244000000231 Sesamum indicum Species 0.000 claims description 5
- 235000003434 Sesamum indicum Nutrition 0.000 claims description 5
- 238000009630 liquid culture Methods 0.000 claims description 5
- -1 Polypropylene Polymers 0.000 claims description 4
- 239000000470 constituent Substances 0.000 claims description 4
- 238000001816 cooling Methods 0.000 claims description 4
- 239000002609 medium Substances 0.000 claims description 4
- 229920000573 polyethylene Polymers 0.000 claims description 4
- 239000002689 soil Substances 0.000 claims description 4
- 241000894007 species Species 0.000 claims description 4
- 238000009423 ventilation Methods 0.000 claims description 4
- 239000004743 Polypropylene Substances 0.000 claims description 3
- 239000001768 carboxy methyl cellulose Substances 0.000 claims description 3
- 239000008112 carboxymethyl-cellulose Substances 0.000 claims description 3
- 235000015097 nutrients Nutrition 0.000 claims description 3
- 229920001155 polypropylene Polymers 0.000 claims description 3
- 238000004659 sterilization and disinfection Methods 0.000 claims description 3
- 230000002159 abnormal effect Effects 0.000 claims description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 2
- 238000009413 insulation Methods 0.000 claims description 2
- 229910052760 oxygen Inorganic materials 0.000 claims description 2
- 239000001301 oxygen Substances 0.000 claims description 2
- 238000012549 training Methods 0.000 claims description 2
- 238000005303 weighing Methods 0.000 claims description 2
- 235000013399 edible fruits Nutrition 0.000 claims 1
- 239000012530 fluid Substances 0.000 claims 1
- 239000010985 leather Substances 0.000 claims 1
- 230000008901 benefit Effects 0.000 abstract description 7
- 239000004615 ingredient Substances 0.000 abstract description 3
- 239000002699 waste material Substances 0.000 abstract description 3
- 238000005516 engineering process Methods 0.000 abstract description 2
- 230000000694 effects Effects 0.000 description 15
- 108090000790 Enzymes Proteins 0.000 description 10
- 102000004190 Enzymes Human genes 0.000 description 10
- 241000233866 Fungi Species 0.000 description 9
- 229940088598 enzyme Drugs 0.000 description 7
- 238000012360 testing method Methods 0.000 description 7
- 241000222336 Ganoderma Species 0.000 description 6
- 108010029541 Laccase Proteins 0.000 description 5
- 238000012258 culturing Methods 0.000 description 5
- 229910052799 carbon Inorganic materials 0.000 description 4
- 235000013599 spices Nutrition 0.000 description 4
- 230000009466 transformation Effects 0.000 description 4
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 3
- 241000222341 Polyporaceae Species 0.000 description 3
- 150000004676 glycans Chemical class 0.000 description 3
- 238000011534 incubation Methods 0.000 description 3
- 238000011835 investigation Methods 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 239000010815 organic waste Substances 0.000 description 3
- 229920001282 polysaccharide Polymers 0.000 description 3
- 239000005017 polysaccharide Substances 0.000 description 3
- 239000002994 raw material Substances 0.000 description 3
- 238000004064 recycling Methods 0.000 description 3
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 2
- 238000009826 distribution Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 241000222382 Agaricomycotina Species 0.000 description 1
- 241000221198 Basidiomycota Species 0.000 description 1
- 244000025254 Cannabis sativa Species 0.000 description 1
- 108010059892 Cellulase Proteins 0.000 description 1
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 244000287680 Garcinia dulcis Species 0.000 description 1
- 229920002488 Hemicellulose Polymers 0.000 description 1
- 206010062717 Increased upper airway secretion Diseases 0.000 description 1
- 102000011782 Keratins Human genes 0.000 description 1
- 108010076876 Keratins Proteins 0.000 description 1
- 208000012902 Nervous system disease Diseases 0.000 description 1
- 239000004698 Polyethylene Substances 0.000 description 1
- AUNGANRZJHBGPY-SCRDCRAPSA-N Riboflavin Chemical compound OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-SCRDCRAPSA-N 0.000 description 1
- 240000005319 Sedum acre Species 0.000 description 1
- 235000014327 Sedum acre Nutrition 0.000 description 1
- 241001091465 Sempervivum Species 0.000 description 1
- 229930003268 Vitamin C Natural products 0.000 description 1
- 229930003316 Vitamin D Natural products 0.000 description 1
- QYSXJUFSXHHAJI-XFEUOLMDSA-N Vitamin D3 Natural products C1(/[C@@H]2CC[C@@H]([C@]2(CCC1)C)[C@H](C)CCCC(C)C)=C/C=C1\C[C@@H](O)CCC1=C QYSXJUFSXHHAJI-XFEUOLMDSA-N 0.000 description 1
- 244000248021 Vitex negundo Species 0.000 description 1
- 235000010363 Vitex negundo Nutrition 0.000 description 1
- 244000029684 Vitex negundo var. negundo Species 0.000 description 1
- LPQOADBMXVRBNX-UHFFFAOYSA-N ac1ldcw0 Chemical compound Cl.C1CN(C)CCN1C1=C(F)C=C2C(=O)C(C(O)=O)=CN3CCSC1=C32 LPQOADBMXVRBNX-UHFFFAOYSA-N 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 238000005273 aeration Methods 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000036772 blood pressure Effects 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 229940106157 cellulase Drugs 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003610 charcoal Substances 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003245 coal Substances 0.000 description 1
- FDJOLVPMNUYSCM-WZHZPDAFSA-L cobalt(3+);[(2r,3s,4r,5s)-5-(5,6-dimethylbenzimidazol-1-yl)-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl] [(2r)-1-[3-[(1r,2r,3r,4z,7s,9z,12s,13s,14z,17s,18s,19r)-2,13,18-tris(2-amino-2-oxoethyl)-7,12,17-tris(3-amino-3-oxopropyl)-3,5,8,8,13,15,18,19-octamethyl-2 Chemical compound [Co+3].N#[C-].N([C@@H]([C@]1(C)[N-]\C([C@H]([C@@]1(CC(N)=O)C)CCC(N)=O)=C(\C)/C1=N/C([C@H]([C@@]1(CC(N)=O)C)CCC(N)=O)=C\C1=N\C([C@H](C1(C)C)CCC(N)=O)=C/1C)[C@@H]2CC(N)=O)=C\1[C@]2(C)CCC(=O)NC[C@@H](C)OP([O-])(=O)O[C@H]1[C@@H](O)[C@@H](N2C3=CC(C)=C(C)C=C3N=C2)O[C@@H]1CO FDJOLVPMNUYSCM-WZHZPDAFSA-L 0.000 description 1
- 238000002485 combustion reaction Methods 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000008030 elimination Effects 0.000 description 1
- 238000003379 elimination reaction Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 230000035784 germination Effects 0.000 description 1
- 230000002218 hypoglycaemic effect Effects 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 238000002386 leaching Methods 0.000 description 1
- 229920005610 lignin Polymers 0.000 description 1
- 238000011068 loading method Methods 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 238000005065 mining Methods 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- 210000005036 nerve Anatomy 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 208000026435 phlegm Diseases 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 150000003254 radicals Chemical class 0.000 description 1
- 230000001850 reproductive effect Effects 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 239000004575 stone Substances 0.000 description 1
- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 125000000391 vinyl group Chemical group [H]C([*])=C([H])[H] 0.000 description 1
- 229920002554 vinyl polymer Polymers 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 235000019154 vitamin C Nutrition 0.000 description 1
- 239000011718 vitamin C Substances 0.000 description 1
- 235000019166 vitamin D Nutrition 0.000 description 1
- 239000011710 vitamin D Substances 0.000 description 1
- 150000003710 vitamin D derivatives Chemical class 0.000 description 1
- 229940046008 vitamin d Drugs 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D3/00—Calcareous fertilisers
- C05D3/02—Calcareous fertilisers from limestone, calcium carbonate, calcium hydrate, slaked lime, calcium oxide, waste calcium products
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Inorganic Chemistry (AREA)
- Mycology (AREA)
- Environmental Sciences (AREA)
- Mushroom Cultivation (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The present invention provides a kind of method using twigs of the chaste tree bits for major ingredient culture ganoderma lucidum, and innovative point is that the culture medium for cultivating weight composition of ganoderma lucidum includes：Twigs of the chaste tree bits 60%, cotton seed hull 20%, wheat bran 19%, quick lime 1%；Preparation method is that the twigs of the chaste tree are ground into sawdust shape, is equipped with wheat bran and quick lime, material-water ratio 1: 1.4.Further included as twigs of the chaste tree bits for the supporting technology of major ingredient culture ganoderma lucidum：In the liquid spawn culture medium and grain spawn culture medium of ganoderma lucidum, twigs of the chaste tree bits also serve as substrate for induction, add in advance.The present invention takes full advantage of the forestry waste twigs of the chaste tree, forms Mode of Circular, avoids the wasting of resources, reduces cultivation cost, and yield is high, the cycle is short.With significant economic benefit and ecological benefits, have broad application prospects.
Description
Technical field
The invention belongs to edible fungus cultivation technical field, and in particular to it is a kind of comprising the twigs of the chaste tree bits Ganodermataceae Dook culture material and
It is used for the method for cultivating ganoderma lucidum.
Background technology
Ganoderma lucidum (Ganoderma lucidum) belongs to fungus circle in genealogical classification, Basidiomycota, Hymenomycetes, ganoderma lucidum mesh,
Ganodermataceae, Ganoderma, is a kind of very high fungi of medical value.The fungus resource of Chinese Ganodermataceae is especially abundant, belongs to now with 4
98 kinds.Ganoderma lucidum is otherwise known as " polyporus lucidus ", " celestial grass ", " seocho ", is also houseleek, whorlleaf stonecrop herb, there is long medicine in China
Use history.Contain monose and polysaccharide in ganoderma lucidum carbohydrate, wherein mainly polysaccharide, the polysaccharide that work constitute about ganoderma lucidum carbon
The 2.5%-5% of hydrate.Amino acid content is very high in ganoderma lucidum, and species is very much, has very high nutritive value.It is in addition, clever
Containing a variety of vitamins in sesame, including vitamin C, B1、B2、B12And vitamin D, they are the indispensable battalion of human body
Support material.Ganoderma lucidum fruitbody, conidia powder and its extract are safe and do not have toxic side effect, have antitumor；Body is adjusted to exempt from
Epidemic disease power；Calmness, calm the nerves；Blood pressure lowering；Antibechic, eliminating the phlegm；Hypoglycemic, elimination free radical；Prevent many sides such as disease of nervous system
The pharmacological action in face, ganoderma lucidum market prospects are boundless at present.
Cotton seed hull is the most common culture material of edible mushroom high-yield culturing because its is full of nutrition, aeration is good, but due in recent years
Come cotton seed hull rise in price, the rise of planting edible mushroom cost, economic benefit declines, so finding can replace the cultivation of cotton seed hull new
Raw material, becomes a big problem at present in the industry, meanwhile, it is also beneficial to the recycling exploitation of other agriculture and forestry organic waste materials.
The twigs of the chaste tree (Vitex negundo var ineica) popular name Jing Zi, the chaste tree tip, strip etc., classification belongs to Verenaceae, male
Chaste tree category, a mutation for five-leaved chaste tree, machaka or dungarunga, several times whole nations of distribution, especially with the coastal area of Jiaodong Peninsula more
Significantly, the Ji Beishan Mountain, southeast Shanxi mountainous region, Taihang Mountain, the ground such as the western mountain and hill in Henan and Shanxi Shan loess plateau is distributed.In river
Northern hilly region in Taihang Mountains distribution is extremely wide, constitutes the dominant shrub species seeds that early tailo is done in the area.We are using generaI investigation
The method combined with focal investigation is investigated, and the twigs of the chaste tree are machaka, and light, drought resisting impoverishment tolerant are thin, are mainly grown in mountain
The ground such as slope, roadside, border earthen mound.Twigs of the chaste tree adaptability is extremely strong, not tight to soil requirement, has growth on all kinds of soil.Twigs of the chaste tree kind
Sub- rudiment power is strong, and Seedling propagation is easy, artificially closes the deserted mountain containing a small amount of twigs of the chaste tree weeds etc., 3-5 can plants burst out in profusion.Hebei
Taihang Mountain twigs of the chaste tree aboundresources, it is widely distributed, it is according to investigations to be in wild state more, larger utilization is there is no, it is main at present to utilize way
Footpath is:
(1) chaste tree is compiled:The twigs of the chaste tree producing region masses, adopt bar and weave various basket baskets, based on personal, seldom export trade.In addition, some are small
Moulded coal is mining, and the twigs of the chaste tree weave float, do frame use for tunnel top surface, but dosage is seldom.
(2) firewood charcoal:The twigs of the chaste tree have the characteristics that resistance to stump, Fuelwood product are high, so local use as combustion bavin more, but utilization
It is extremely limited.
For traditional Land use systems chaste tree is compiled, total utilizations of the twigs of the chaste tree also insufficient stock number 10%, research shows the twigs of the chaste tree
In have nutriment necessary to the growth of cellulose, lignin, hemicellulose these edible and medicinal fungis, also containing abundant organic matter
With the required nutrient of crop growth.But up to the present, the twigs of the chaste tree, which are always treated as agriculture and forestry organic waste material, not to be had
To fully effectively developing, resource is seriously wasted.As low-carbon environment-friendly and living resources recycle idea
Deeply, it using twigs of the chaste tree culturing edible fungus, can not only the twigs of the chaste tree is turned waste into wealth, it is substituted traditional cultivation matrix cotton seed hulls, into
For the new medium of culturing edible fungus, while more opportunities are created for increasing peasant income.
The content of the invention
The object of the present invention is to provide a kind of method using twigs of the chaste tree culture ganoderma lucidum, solves the wild chaste tree of current Taihang mountain areas
The problem of bar wastes, realizes the living resources circulation profit of agriculture and forestry organic waste material on the premise of environmentally safe and high yield
With.
In order to realize purpose of the present invention, the present invention provides following technical scheme.
This method for considering culture ganoderma lucidum to be worth doing using the twigs of the chaste tree, innovative point are that the culture medium for cultivating weight composition of ganoderma lucidum includes：
Twigs of the chaste tree bits 60%, cotton seed hull 20%, wheat bran 19%, quick lime 1%；Preparation method is that the twigs of the chaste tree are ground into sawdust, is equipped with wheat bran
And quick lime, material-water ratio 1: 1.4.
Described considers the method for cultivating ganoderma lucidum to be worth doing using the twigs of the chaste tree, and substrate for induction is also served as in the liquid spawn culture medium of ganoderma lucidum
Thing, add twigs of the chaste tree bits in advance；It optimizes liquid spawn culture medium formulation weight ratio：Potato 20%, twigs of the chaste tree powder 5%, wheat bran
3%, glucose 2%, KH2PO40.3%, MgSO40.15%, VB110mg/L, carboxymethyl cellulose 0.3%, water 1000mL, pH
It is natural.
Described considers the method for cultivating ganoderma lucidum to be worth doing using the twigs of the chaste tree, and substrate for induction is also served as in the grain spawn culture medium of ganoderma lucidum
Thing, add twigs of the chaste tree bits in advance；Its optimize grain spawn culture medium prescription weight ratio be：Wheat 69%, twigs of the chaste tree bits 30%, raw stone
Ash 1%.
The method for being considered to be worth doing culture ganoderma lucidum using the twigs of the chaste tree, is included the following steps：
A. the preparation of liquid spawn
A. the preparation of level liquid strain
Under aseptic condition, commercially available parent species are accessed in level liquid culture medium, 28 DEG C, 160r/min constant-temperature shaking cultures
5d, that is, be made level liquid strain；
The level liquid bacterium culture medium includes：By weight percentage, potato 20%, wheat bran 3%, glucose
2%, KH2PO40.3%, MgSO40.15%, CMC0.3%, VB110mg/L；
B. the preparation of secondary liquid strain
Under aseptic condition, level liquid strain is accessed in secondary liquid culture medium with 10% inoculum concentration, 28 DEG C,
160r/min constant-temperature shaking culture 7d, that is, be made secondary liquid strain；
The secondary liquid Spawn incubation based formulas is：By weight percentage, potato 20%, twigs of the chaste tree bits powder 5%, bran
Skin 3%, glucose 2%, KH2PO40.3%, MgSO40.15%, CMC0.3%, VB110mg/L；
B. the preparation of grain spawn
The secondary liquid strain that step A is obtained accesses grain spawn culture medium, inoculum concentration 10%, 22-25 DEG C of culture
To completely, grain spawn is obtained；
The grain spawn culture medium includes：Wheat 69%, twigs of the chaste tree bits 30%, quick lime 1%.
C. the preparation of twigs of the chaste tree bits compost
It is sawdust shape that twigs of the chaste tree branch crushes in advance；
Twigs of the chaste tree bits compost composition includes：Twigs of the chaste tree bits 60%, cotton seed hull 20%, wheat bran 19%, quick lime 1%；
The accurate each constituent for weighing twigs of the chaste tree bits culture medium, after being mixed with water, vexed 30 min of heap, loads container
Interior, sterilizing is spare after cooling；
D. it is inoculated with, cultivates, harvesting
Under aseptic condition, the grain spawn access step C that step B the is prepared twigs of the chaste tree being prepared are considered to be worth doing compost,
Cultivated, management of producing mushroom, harvesting.
The method for considering culture ganoderma lucidum to be worth doing using the twigs of the chaste tree, the inoculum concentration of grain spawn is 5-10% in step D.
It is described using twigs of the chaste tree bits culture ganoderma lucidum method, contain in step C twigs of the chaste tree bits compost container select Polythene Bag,
Polypropylene Bag or vial.
The method for considering culture ganoderma lucidum to be worth doing using the twigs of the chaste tree, the sterilising conditions of twigs of the chaste tree bits compost are 121 DEG C of high pressures in step C
Sterilize 150min or 100 DEG C of normal-pressure sterilization 24h.
The present invention is used for the culture medium of cultivating ganoderma, and using twigs of the chaste tree bits as primary raw material, effect has three：1st, for edible mushroom and
Speech, carbon source needed for growth is provided for it.2nd, the twigs of the chaste tree are not yet developed at present, and aboundresources, provides for edible fungus culturing
A kind of cheap culturing raw material, production cost is low, high financial profit.3rd, from the perspective of ecology, agricultural are realized
The recycling of discarded object, has considerable economic benefit, has a extensive future.
Embodiment
Content of the present invention is further described in detail with reference to specific embodiment.
Embodiment 1, consider the method for cultivating ganoderma lucidum to be worth doing using the twigs of the chaste tree, and lucidum strain is market purchasing
A. the preparation of liquid spawn
Level liquid Spawn incubation based formulas：Potato 20%, wheat bran 3%, glucose 2%, KH2PO40.3%,
MgSO40.15%, VB110mg/L, CMC0.3%, pH are natural；
Secondary liquid Spawn incubation based formulas：Twigs of the chaste tree bits powder 5%, potato 20%, wheat bran 3%, glucose 2%,
KH2PO40.3%, MgSO40.15%, VB110mg/L, CMC0.3%, pH are natural；Wherein twigs of the chaste tree bits crushed 80 mesh sieves and obtain powder
Shape, boils 30min, filter and remove residue obtains leaching liquor altogether with potato, wheat bran.
Level liquid strain uses 500mL conical flasks when preparing, after liquid amount 200mL, 121 DEG C of autoclaving 30min,
Commercially available PDA slant strains are accessed under aseptic condition, every bottle of inoculum concentration is 4 pieces of 0.5cm × 0.5cm strain blocks.28 after inoculation
DEG C, under conditions of 160r/min, constant-temperature shaking culture 5d.
Secondary liquid strain liquid amount specification, sterilising conditions and condition of culture are same as above, inoculum concentration 10%.
Laccase activity and carboxymethyl cellulose in secondary liquid strain preparatory phase, measure optimization liquid fungus seed liquid
Enzyme enzyme activity (CMC enzyme activity), to be not added with the culture medium of twigs of the chaste tree crumbs powder as control, to find that twigs of the chaste tree powder is in liquid spawn
The no advantage for thering is substrate for induction to act on.The results are shown in Table 1 for enzyme activity determination.
Laccase activity defines：Under certain condition, the enzyme amount per minute for making reaction solution OD values increase by 0.001 is defined as one
A laccase activity unit of force (U).
Carboxymethylcelluloenzyme enzyme enzyme activity (CMC enzyme activity) defines：Under certain condition, enzymolysis substrate per minute produces 1 μ g
The enzyme amount of glucose is defined as a carboxymethylcelluloenzyme enzyme enzyme activity unit (U).
The enzyme-activity data of 1 ganoderma strain of table
As shown in Table 1, the laccase activity of secondary liquid culture medium of the present invention, CMC enzyme activity (are not added with than control group
The twigs of the chaste tree are considered to be worth doing) height, it is seen that secretion of the twigs of the chaste tree bits to the laccase and cellulase of Ganoderma lucidum mycelium is added in liquid medium within and is played
Certain substrate for induction effect, allows mycelia to adapt to substrate (twigs of the chaste tree bits) in advance, so as to accelerate long speed of the mycelia in culture material, contracting
Short cultivation period.
B. the preparation of grain spawn
Grain spawn culture medium prescription：Wheat 69%, twigs of the chaste tree bits 30%, quick lime 1%.
Accurate to weigh each constituent of culture medium, 1 ︰ 1.4 of material-water ratio carries out spice, loads 17cm × 32cm × 0.05cm and gathers
Vinyl bag, per packed siccative 300g, prepares 120 bags.Obtained secondary liquid strain will be optimized and be not added with twigs of the chaste tree bits
Control group liquid spawn be respectively connected to grain spawn culture medium, be inoculated with 60 bags respectively, measure bacterium length speed, and when recording full shoulder
Between, the purseful time.
In order to investigate the advantage of grain spawn culture medium of the present invention, the present invention has carried out contrast test, compares tissue culture
It is wheat culture medium to support base：Wheat 99%, quick lime 1%；Comparative test result is as shown in table 2.
The purseful time of 2 ganoderma lucidum grain spawn of table
As shown in Table 2, mycelia is faster than the long speed of pure wheat culture medium in " wheat+twigs of the chaste tree bits " grain culture medium, this be because
To add twigs of the chaste tree bits in liquid medium within, enable mycelia faster Adaptable growth substrate, so as to accelerate bacterium germination speed, shorten life
Produce the cycle and improve production efficiency.To sum up, wheat+twigs of the chaste tree bits can replace cotton seed hull to become the master that ganoderma lucidum plantation Central Plains kind makes
Material, this discarded object of the twigs of the chaste tree have also obtained recycling, have been of great importance to the Efficient Cultivation of ganoderma lucidum.
C. the preparation of twigs of the chaste tree bits compost
In mass ratio, twigs of the chaste tree bits 60%, cotton seed hull 20%, wheat bran 19%, quick lime 1% are weighed, quick lime 1%, expects water
Than carrying out spice for 1 ︰ 1.4, after mixing, it is fitted into after vexed 30 min of heap in container and is sterilized and be inoculated with work.
Above-mentioned compost is loaded in Polythene Bag, Polypropylene Bag or vial, bore is unlimited, and conventional tying, high pressure is gone out
Bacterium is generally with 121 DEG C of sterilizing 150min；If normal-pressure sterilization generally with 100 DEG C of sterilizings, keeps 24h；It is inoculated with after cooling.
The present invention in the course of the research investigates the dosage of twigs of the chaste tree bits, specific as follows：
Each constituent is accurately weighed as shown in table 3, is mixed, adds water, material-water ratio is 1 ︰ 1.4, the training configured
Nutriment loads test tube, and each formula is repeated 5 times, 121 DEG C of 150 min of autoclaving.Grain spawn described in step B is respectively connected to
In test tube containing compost, test tube nutrient growth experiment is carried out.
3 culture medium prescription of table (siccative, %)
| Formula | The twigs of the chaste tree are considered to be worth doing | Cotton seed hull | Wheat bran | Quick lime |
| P0 | 0 | 20 | 19 | 1 |
| P1 | 30 | 20 | 19 | 1 |
| P2 | 40 | 20 | 19 | 1 |
| P3 | 50 | 20 | 19 | 1 |
| P4 | 60 | 20 | 19 | 1 |
| P5 | 70 | 20 | 19 | 1 |
| P6 | 0 | 80 | 19 | 1 |
Every the long speed and growing way situation of mycelia in the test tube of observation in 2 days, surveyed with vernier caliper (accuracy 0.02mm)
Determine the long speed of mycelia.The results are shown in Table 4 for Growth rata and potential of hypha.
The mycelial growth situation of 4 ganoderma lucidum of table
| Formula | Mycelium length (mm/d) | Mycelium growth vigor |
| P6 | 7.65±0.01 | +++ |
| P4 | 7.21±0.02 | +++ |
| P5 | 7.15±0.03 | +++ |
| P3 | 6.92±0.05 | +++ |
| P1 | 6.47±0.04 | ++ |
| P2 | 6.15±0.01 | ++ |
| P0 | 6.03±0.02 | + |
(note：It is average value ± standard error to list long speed；+++ represent that growth is fine and close, ++ represent that growth is finer and close ,+represent
Growth is general.)
As can be seen from Table 4, when twigs of the chaste tree bits additive amount is increased slightly, its growing way is preferable, and mycelia is pure white, finer and close, with
Pure cotton seed peel culture medium is comparable, and mycelia grows most fast on formula P6.
Each component is weighed by the formula in table 3, is uniformly mixed, 1 ︰ 1.4 of material-water ratio progress spices, loading 17cm × 32cm ×
0.05cm polyethylene plastic bags, per about 300 g of packed siccative, inoculum concentration is " liquid spawn → twigs of the chaste tree bits+cotton seed hull " production of hybrid seeds
Grain spawn one is inoculated with spoon, observes and records the growing state of each formula and calculate biological transformation ratio.The result of the test such as institute of table 5
Show.
The biological transformation ratio of 5 ganoderma lucidum of table
| Culture medium is numbered | Average product (g) | Average organism efficiency (%) |
| P6 | 223.62 | 74.54 |
| P4 | 219.30 | 73.10 |
| P3 | 194.85 | 64.95 |
| P5 | 186.9 | 62.30 |
| P1 | 179.64 | 59.88 |
| P2 | 166.29 | 55.43 |
| P0 | 155.16 | 51.72 |
As can be seen from Table 5, when reaching 60% by primary carbon source of twigs of the chaste tree bits, the biological transformation ratio of ganoderma strain reaches
73.10%, although the not up to biological transformation ratio 74.54% of culture material major ingredient pure cotton seed peel, is comparable.Illustrate the twigs of the chaste tree
Bits can partly replace cotton seed hull cultivating ganoderma.
Finally definite Optimum formulae of the invention is twigs of the chaste tree bits 60%, cotton seed hull 20%, wheat bran 19%, quick lime 1%, expects water
Than carrying out spice for 1 ︰ 1.4.
D. it is inoculated with, cultivates, harvesting
To be sterilized fruiting bag after cooling, according to grain bacterium obtained by the inoculum concentration access step B of 5-10% under sterile working
Kind.The bacterium bag for connecting kind is placed in 25-28 DEG C of progress lucifuge culture at room temperature, strain sprouted, be colonized as early as possible.Treat mycelia purseful
Afterwards, open wide sack and carry out reproductive growth management phase.
Canopy temperature is maintained at 26-30 DEG C when going out sesame, and relative air humidity wants 90%-95%, and provides scattering light and fill
The oxygen of foot.Keep ground to have shoaling layer, to spray water once to soil surrounding and space daily.During every morning 8 before and
Isolated film is raised after during afternoon 4 and carrys out ventilating opening ventilation.Former base has been expanded 3-5 days, slowly forms cap, to increase water spray
Insulation, if the excessive temperature control that to spray water of temperature.If improper ventilation easily goes out lopsided sesame, to be sliced off at once if there is abnormal bud.
When cap is by white-pale yellow-yellow liter bronzing, the edge white of cap is basic to disappear, and edge starts to redden, cap keratin
Change, when the cap back side has ejected vaporific spore russet, illustrate that ganoderma lucidum fruitbody is ripe, so that it may harvesting in time.
In conclusion using the twigs of the chaste tree as the living resources of reusable edible, the industrialization of Ganoderma is eaten for high temperature modification
Exploitation, has important innovative significance.
Claims (4)
1. a kind of method for being considered to be worth doing culture ganoderma lucidum using the twigs of the chaste tree, is included the following steps：
A. the preparation of liquid spawn
A. the preparation of level liquid strain
Under aseptic condition, commercially available parent species are accessed in level liquid culture medium, 28 DEG C, 160r/min constant-temperature shaking culture 5d, i.e.,
Level liquid strain is made；
The level liquid bacterium culture medium includes：By weight percentage, potato 20%, wheat bran 3%, glucose 2%,
KH2PO40.3%, MgSO40.15%, CMC 0.3%, VB110mg/L；
B. the preparation of secondary liquid strain
Twigs of the chaste tree bits are added in advance as substrate for induction thing in the liquid spawn culture medium of ganoderma lucidum；It optimizes liquid spawn culture medium and matches somebody with somebody
Square weight ratio is：Potato 20%, twigs of the chaste tree powder 5%, wheat bran 3%, glucose 2%, KH2PO40.3%, MgSO40.15%,
VB110mg/L, carboxymethyl cellulose 0.3%, water 1000mL, pH are natural；Twigs of the chaste tree bits used, crush in advance in fluid nutrient medium
And cross 80 mesh sieves；
Under aseptic condition, level liquid strain is accessed in secondary liquid culture medium with 10% inoculum concentration, 28 DEG C, 160r/min
Constant-temperature shaking culture 7d, that is, be made secondary liquid strain；
B. the preparation of grain spawn
Substrate for induction thing is also served as in the grain spawn culture medium of ganoderma lucidum, adds twigs of the chaste tree bits in advance；Its grain spawn optimized training
Supporting based formulas weight ratio is：Wheat 69%, twigs of the chaste tree bits 30%, quick lime 1%, twigs of the chaste tree bits used, thing in grain spawn culture medium
It is sawdust shape first to crush；
The secondary liquid strain that step A is obtained accesses grain spawn culture medium, inoculum concentration 10%, 22-25 DEG C of culture to full,
Obtain grain spawn；
C. the preparation of twigs of the chaste tree bits compost
Twigs of the chaste tree bits compost composition includes：Twigs of the chaste tree bits 60%, cotton seed hull 20%, wheat bran 19%, quick lime 1%；Preparation method is
The twigs of the chaste tree are ground into sawdust, are equipped with wheat bran and quick lime, material-water ratio 1: 1.4；
The accurate each constituent for weighing twigs of the chaste tree bits culture medium, after being mixed with water, the vexed 30min of heap, loads in container, goes out
Bacterium, it is spare after cooling；
D. it is inoculated with, cultivates, harvesting
Under aseptic condition, consider the grain spawn access step C that step B the is prepared twigs of the chaste tree being prepared to be worth doing compost, carry out
Culture, management of producing mushroom, harvesting：
Canopy temperature is maintained at 26-30 DEG C when going out sesame, and relative air humidity wants 90%-95%, and provides scattering light and abundance
Oxygen, will keep ground to have shoaling layer, spray water once to soil surrounding and space daily；
Isolated film is raised after during before during every morning 8 and afternoon 4 and carrys out ventilating opening ventilation；Former base has expanded 3-5 days, slowly
It is slow to form cap, to increase water spray insulation, if the excessive temperature control that to spray water of temperature；If improper ventilation easily goes out lopsided sesame, such as
, which there is abnormal bud, in fruit to be sliced off at once；
When cap is by white-pale yellow-yellow liter bronzing, the edge white of cap is basic to disappear, and edge starts to redden, cap leather
Matter, when the cap back side has ejected vaporific spore russet, illustrates that ganoderma lucidum fruitbody is ripe, so that it may harvesting in time.
2. the method for twigs of the chaste tree bits culture ganoderma lucidum is utilized according to claim 1, it is characterised in that grain spawn in step D
Inoculum concentration is 5-10%.
3. the method for twigs of the chaste tree bits culture ganoderma lucidum is utilized according to claim 1, it is characterised in that twigs of the chaste tree bits are contained in step C
The container of compost selects Polythene Bag, Polypropylene Bag or vial.
4. the method for twigs of the chaste tree bits culture ganoderma lucidum is utilized according to claim 1, it is characterised in that twigs of the chaste tree bits culture in step C
The sterilising conditions of material are 121 DEG C of autoclaving 150min or 100 DEG C of normal-pressure sterilization 24h.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410611995.5A CN104303845B (en) | 2014-11-03 | 2014-11-03 | A kind of method for considering culture ganoderma lucidum to be worth doing using the twigs of the chaste tree |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410611995.5A CN104303845B (en) | 2014-11-03 | 2014-11-03 | A kind of method for considering culture ganoderma lucidum to be worth doing using the twigs of the chaste tree |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104303845A CN104303845A (en) | 2015-01-28 |
| CN104303845B true CN104303845B (en) | 2018-04-27 |
Family
ID=52359286
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410611995.5A Expired - Fee Related CN104303845B (en) | 2014-11-03 | 2014-11-03 | A kind of method for considering culture ganoderma lucidum to be worth doing using the twigs of the chaste tree |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104303845B (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104871829A (en) * | 2015-06-12 | 2015-09-02 | 河北大学 | Method for culturing Clitocybe maxima from cattail scraps |
| CN104871828A (en) * | 2015-06-12 | 2015-09-02 | 河北大学 | Method for culturing ganoderma lucidum from cattail scraps |
| CN107873394A (en) * | 2017-12-05 | 2018-04-06 | 罗成喜 | A kind of implantation methods of ganoderma lucidum |
| CN109845582A (en) * | 2019-01-07 | 2019-06-07 | 贵州正黔门大健康产业有限公司 | A kind of preparation method of Coriaria sinica mushroom liquid bacterial |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH119085A (en) * | 1997-06-20 | 1999-01-19 | Kameda Seika Kk | Culture medium for cultivating mushroom and culture medium for culturing spawn |
| CN1631078A (en) * | 2003-12-23 | 2005-06-29 | 马士贵 | Wild-imitating mushroom edible fungus seed and cultivation method thereof |
| CN102301913B (en) * | 2011-07-11 | 2012-11-07 | 广东省微生物研究所 | Method for artificially cultivating Ganoderma guinanense fruiting bodies |
| CN102835248B (en) * | 2012-08-09 | 2014-07-16 | 颍上县鸿涛菌业专业合作社 | Method for cultivating lucid ganoderma by using mulberry branches |
| CN103694016A (en) * | 2013-12-05 | 2014-04-02 | 何庚挺 | Ganoderma lucidum culture medium and culture method thereof |
| CN104025908B (en) * | 2014-06-27 | 2016-04-20 | 广西仙草堂制药有限责任公司 | Ganoderma lucidum cultivation method |
-
2014
- 2014-11-03 CN CN201410611995.5A patent/CN104303845B/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CN104303845A (en) | 2015-01-28 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103766114B (en) | Selenium-rich fragrant taro cultivating method | |
| CN104429591B (en) | A kind of method utilizing Vitex chinensis Mill. bits to cultivate Auricularia polytricha (Mout) Sacc. | |
| CN104041330B (en) | Ganoderma tsugae imitates wild juggle cultivation method | |
| CN103891524B (en) | The method of glossy ganoderma dish garden formula cultivation and the medium for cultivating ganoderma | |
| CN104478546B (en) | A kind of method utilizing Vitex chinensis Mill. bits to cultivate hedgehog hydnum | |
| CN103125251A (en) | Application method of arbuscular mycorrhizal fungus in large-scale tobacco cultivation | |
| CN107980546A (en) | A kind of blueberry cultivation matrix modifier and its preparation method and application | |
| CN104094774B (en) | A kind of litchi branch that utilizes considers the method for cultivating Agrocybe chaxingu to be worth doing | |
| CN102613054A (en) | Method for improving cold and disease resistance of tobacco | |
| CN102696379A (en) | Floating two-stage osmunda japonica spore growing method | |
| CN106258478B (en) | Morchella esculenta nutrition bag made of straw fermentation substrate and preparation method thereof | |
| CN104303845B (en) | A kind of method for considering culture ganoderma lucidum to be worth doing using the twigs of the chaste tree | |
| CN102379208A (en) | Pleurotus ferulae cultivation technology | |
| CN106399132A (en) | Irpex lacteus and application thereof | |
| CN112021073A (en) | Morchella esculenta external aid nutrition bag ingredient, nutrition bag and preparation method thereof | |
| CN104478547B (en) | A kind of method of the yellow umbrella of utilization twigs of the chaste tree bits culture | |
| CN109006180A (en) | A kind of mushroom culture medium and preparation method thereof | |
| CN105145257B (en) | A kind of golden Chinese scholartree woods builds method | |
| CN105993597A (en) | Cultivation method for coprinus comatus | |
| CN107810782A (en) | A kind of high-yield method of bracteal leaf of corn cultivating ganoderma | |
| CN104855142A (en) | Method for using cattail crumbs to cultivate stropharia rugoso-annulata | |
| CN105165387A (en) | Method for culturing pleurotus geesteranus by using wild-jujube branch sawdust | |
| CN110050665A (en) | A kind of Nutrition Soil and preparation method for desert plant cultivation | |
| CN105027971B (en) | A kind of method using wild jujube twig powder mushroom culture | |
| CN108713449A (en) | A kind of cultural method of Poria cocos |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20180427 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |