CN104273318A - Method for producing bacterial protein based on succinic acid fermentation waste liquid - Google Patents
Method for producing bacterial protein based on succinic acid fermentation waste liquid Download PDFInfo
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- CN104273318A CN104273318A CN201310287286.1A CN201310287286A CN104273318A CN 104273318 A CN104273318 A CN 104273318A CN 201310287286 A CN201310287286 A CN 201310287286A CN 104273318 A CN104273318 A CN 104273318A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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Abstract
A method for producing bacterial protein based on a succinic acid fermentation waste liquid is disclosed. The method comprises: taking the waste liquid generated in the process of producing succinic acid by fermenting white spirit distillers' grains as a raw material, adding a flocculating agent into the fermentation waste liquid, and separating flocculated bacteria, so as to obtain a bacteria concentrated liquid, heating, then performing filtering dewatering, adjusting pH, inoculating geotrichum candidum bacteria to perform solid aerobic fermentation, and then performing separation and drying to obtain the bacteria protein. Compared with conventional bacteria protein, the method mainly comprises two innovation points: (1) the method employs the waste liquid generated in the process of producing succinic acid by fermenting white spirit distillers' grains as the raw material, a new protein resource is added, and the environment pollution problem caused by a large amount of wastewater generated after production of succinic acid is also solved; and (2) the method employs a geotrichum candidum solid aerobic fermentation process, and the bacteria protein prepared by using the process has the protein content higher by 3%-5% than the protein content of bacterial protein prepared by using other methods, and amino acid variety is relatively abundant.
Description
Technical field
The present invention relates to a kind of method of producing mycoprotein based on succinic acid fermented waste fluid, with waste liquid after distillers ' grains fermentation production of succinic acid for raw material, carry out technique and method that solid-state aerobic fermentation produces mycoprotein, belong to technical field of bioengineering.
Background technology
Distillers ' grains is the waste liquid that liquor production fermentation produces, at present only as the use of domestic animal, utilization rate and added value lower, and can occupied ground, summer can produce peculiar smell, contaminated environment.
China is a wine brewing big country, and vinasse resource is very abundant, but the utilization rate of vinasse is but very low, the restraining factors that restriction distillers ' grains resource makes full use of have two: one to be fresh poor acidity large (pH is about 3.8), moisture height 60%-65%, easily putrid and deteriorated, be unfavorable for storage and transport.Two is because white wine adds a large amount of rice husks in alcoholic process, and after measured, in air-dry thing, the weight of rice husk accounts for about 30%, and volume accounts for about 50%, and crude fiber content is up to more than 17.8%, low as digestibility, palatability is poor, is difficult to absorb.
The present invention with waste liquid after distillers ' grains fermentation production of succinic acid for raw material, carry out technique and method that solid-state aerobic fermentation produces mycoprotein, adopt the advanced technologies of bioengineering fermentation technique and production process product complete utilization, zero-emission, develop the mycoprotein product of high protein, high amino acid content.The mycoprotein produced in the present invention, its albumen safety non-toxic, protein content is high, good palatability, can not only promote the development of animal husbandry, but also can solve the awkward situation of white wine producer vinasse process, and this technique and method have good promotional value.
Summary of the invention
For the problems referred to above, the invention provides a kind of method of producing mycoprotein based on succinic acid fermented waste fluid, its object is to: new protein sources can be developed, promote the development of animal husbandry, but also the awkward situation of the vinasse process of white wine producer and succinic acid Wastewater treating can be solved.
Technical solution of the present invention:
A kind of method of producing mycoprotein based on succinic acid fermented waste fluid, it is characterized in that: described mycoprotein is for raw material with the waste liquid of distillers ' grains fermentation production of succinic acid generation, flocculant mixing is added in fermented waste fluid, the thalline of flocculation is separated, obtain concentration liquid, carry out filtering means dehydration again after heating, then regulate pH, inoculation geotrichum candidum thalline carries out solid-state aerobic fermentation, then carries out being separated, dry and obtain.
The preparation technology of described mycoprotein is as follows:
(1) pretreatment: the wheat bran adding about 13%-17% in the poor slag after dehydration is separated.
(2) adjust pH: the pH to 5.5-5.9 regulating poor liquid with the pulverized limestone of 1.5%-2%, add the urea of 1%-1.5% as nitrogenous source simultaneously, regulate the moisture of compost to moisture content 60%-70%.
(3) production of hybrid seeds: geotrichum candidum bacterial classification, with liquid seed production way, carries out the production of hybrid seeds according to the order of inclined-plane kind activation → one-level shaking flask kind → second-level shake flask kind → seedlet tank → large seeding tank.
(4) inoculation fermentation: the compost after nutrition-allocated proportion is inoculated above-mentioned geotrichum candidum liquid spawn, and inoculum concentration is 5%-10%, and fermentation temperature remains on 28-33 DEG C, ventilates, fermentation 24-36 hour.
(5) be separated: the mode of the mycoprotein plate compression after fermentation is separated.
(6) dry: filtrate is carried out pneumatic conveying drying, and temperature of charge is no more than 65 DEG C.
(7) finished product: dried mycoprotein carried out pulverizing and crosses 40 mesh sieves, getting product after packaging.
Beneficial effect of the present invention:
Advantage of the present invention is the waste liquid after utilizing distillers ' grains fermentation production of succinic acid is raw material, by adding appropriate carbon source and nitrogenous source, inoculation geotrichum candidum bacterial classification carries out solid-state aerobic fermentation and produces mycoprotein, both can prevent vinasse and fermented waste fluid contaminated environment, improve again the use value of fermented waste fluid.The mycoprotein crude protein content that the present invention produces is more than 35%, and amino acid content is more than 23%.
The present invention not only can develop new protein sources, promotes the development of animal husbandry, but also can solve the awkward situation of the vinasse process of white wine producer and succinic acid Wastewater treating.Therefore this production technology and method have good promotional value.
Compare to existing mycoprotein, the innovation of two aspects is mainly contained: 1. on raw material in the present invention, what the present invention adopted is for raw material with the waste liquid produced after distillers ' grains fermentation succinic acid, not only increase new protein sources, but also solve a large amount of waste water pollution on the environment problems produced after succinic acid is produced; 2., in technique, what the present invention adopted is the method for geotrichum candidum solid-state aerobic fermentation, and the mycoprotein protein content obtained by the method exceeds 3%-5% than additive method, and amino acid classes is abundanter.
Accompanying drawing explanation
Fig. 1: present invention process flow chart.
Detailed description of the invention
Below in conjunction with drawings and Examples, the present invention is described further:
A kind of method of producing mycoprotein based on succinic acid fermented waste fluid, described mycoprotein is for raw material with the waste liquid of distillers ' grains fermentation production of succinic acid generation, flocculant mixing is added in fermented waste fluid, the thalline of flocculation is separated, obtain concentration liquid, carry out filtering means dehydration again after heating, then regulate pH, inoculation geotrichum candidum thalline carries out solid-state aerobic fermentation, then carries out being separated, dry and obtain.
The preparation technology of described mycoprotein is as follows:
(1) pretreatment: the carbon source that can be used for microorganism utilization in the waste liquid due to distillers ' grains fermentation production of succinic acid is less, therefore the wheat bran carbon source as a supplement of about 13%-17% is added in the poor slag after being separated that dewaters.
(2) pH is adjusted: because the acidity of poor liquid is comparatively large, is unfavorable for normal fermentation, therefore the pH to 5.5-5.9 of poor liquid need be regulated with the pulverized limestone of 1.5%-2%.Add the urea of 1%-1.5% as nitrogenous source simultaneously, regulate the moisture of compost to moisture content 60%-70%.
(3) production of hybrid seeds: geotrichum candidum bacterial classification, with liquid seed production way, carries out the production of hybrid seeds according to the order of inclined-plane kind activation → one-level shaking flask kind → second-level shake flask kind → seedlet tank → large seeding tank.
(4) inoculation fermentation: the compost after nutrition-allocated proportion is inoculated above-mentioned geotrichum candidum liquid spawn, and inoculum concentration is 5%-10%, and fermentation temperature remains on 28-33 DEG C, ventilates, fermentation 24-36 hour.
(5) be separated: the mode of the mycoprotein plate compression after fermentation is separated.
(6) dry: filtrate is carried out pneumatic conveying drying, and temperature of charge is no more than 65 DEG C.
(7) finished product: dried mycoprotein carried out pulverizing and crosses 40 mesh sieves, getting product after packaging.
Embodiment one:
Get the waste liquid of 100kg distillers ' grains fermentation production of succinic acid, add the wheat bran carbon source as a supplement of about 13% wherein, the pH to 5.5 of poor liquid is regulated again with the pulverized limestone of 1.5%, add the urea of 1% as nitrogenous source simultaneously, regulate the moisture of compost to moisture content 60%, the geotrichum candidum adding 5% carries out solid-state aerobic fermentation 24 hours, the mode of the mycoprotein plate compression after fermentation is separated, filtrate is carried out pneumatic conveying drying, temperature of charge 58 DEG C, mycoprotein moisture is less than 10%, is carried out pulverizing by dried mycoprotein and cross 40 mesh sieves getting product.The product protein content that example is obtained is thus 33.5%.
Embodiment two:
Get the waste liquid of 100kg distillers ' grains fermentation production of succinic acid, add the wheat bran carbon source as a supplement of about 15% wherein, the pH to 5.7 of poor liquid is regulated again with the pulverized limestone of 1.8%, add the urea of 1.3% as nitrogenous source simultaneously, regulate the moisture of compost to moisture content 65%, the geotrichum candidum adding 8% carries out solid-state aerobic fermentation 30 hours, the mode of the mycoprotein plate compression after fermentation is separated, filtrate is carried out pneumatic conveying drying, temperature of charge 60 DEG C, mycoprotein moisture is less than 12%, is carried out pulverizing by dried mycoprotein and cross 40 mesh sieves getting product.The product protein content that example is obtained is thus 34.3%.
Embodiment three:
Get the waste liquid of 100kg distillers ' grains fermentation production of succinic acid, add the wheat bran carbon source as a supplement of about 17% wherein, the pH to 5.9 of poor liquid is regulated again with the pulverized limestone of 2.0%, add the urea of 1.5% as nitrogenous source simultaneously, regulate the moisture of compost to moisture content 70%, the geotrichum candidum adding 10% carries out solid-state aerobic fermentation 36 hours, the mode of the mycoprotein plate compression after fermentation is separated, filtrate is carried out pneumatic conveying drying, temperature of charge 62 DEG C, mycoprotein moisture is less than 15%, is carried out pulverizing by dried mycoprotein and cross 40 mesh sieves getting product.The product protein content that example is obtained is thus 35.2%.
To sum up, the present invention accomplishes the end in view.
Claims (4)
1. produce a method for mycoprotein based on succinic acid fermented waste fluid, it is characterized in that: the preparation technology of described mycoprotein is as follows:
(1) pretreatment: the wheat bran adding about 13%-17% in the poor slag after dehydration is separated.
(2) adjust pH: the pH to 5.5-5.9 regulating poor liquid with the pulverized limestone of 1.5%-2%, add the urea of 1%-1.5% as nitrogenous source simultaneously, regulate the moisture of compost to moisture content 60%-70%.
(3) production of hybrid seeds: geotrichum candidum bacterial classification, with liquid seed production way, carries out the production of hybrid seeds according to the order of inclined-plane kind activation → one-level shaking flask kind → second-level shake flask kind → seedlet tank → large seeding tank.
(4) inoculation fermentation: the compost after nutrition-allocated proportion is inoculated above-mentioned geotrichum candidum liquid spawn, and inoculum concentration is 5%-10%, and fermentation temperature remains on 28-33 DEG C, ventilates, fermentation 24-36 hour.
(5) be separated: the mode of the mycoprotein plate compression after fermentation is separated.
(6) dry: filtrate is carried out pneumatic conveying drying, and temperature of charge is no more than 65 DEG C.
(7) finished product: dried mycoprotein carried out pulverizing and crosses 40 mesh sieves, getting product after packaging.
2. a kind of method of producing mycoprotein based on succinic acid fermented waste fluid according to claim 1, is characterized in that: add the wheat bran of about 15% in the poor slag in described step (1) after dehydration is separated.
3. a kind of method of producing mycoprotein based on succinic acid fermented waste fluid according to claim 1, it is characterized in that: the pH to 5.8 regulating poor liquid in described step (2) with the pulverized limestone of 1.8%, add the urea of 1.3% as nitrogenous source simultaneously, regulate the moisture of compost to moisture content 65%.
4. a kind of method of producing mycoprotein based on succinic acid fermented waste fluid according to claim 1, it is characterized in that: in described step (4), the compost after nutrition-allocated proportion is inoculated above-mentioned geotrichum candidum liquid spawn, inoculum concentration is 8%, and fermentation temperature remains on 30 DEG C, ventilate, ferment 30 hours.
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| CN201310287286.1A CN104273318A (en) | 2013-07-10 | 2013-07-10 | Method for producing bacterial protein based on succinic acid fermentation waste liquid |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1285156A (en) * | 1999-08-23 | 2001-02-28 | 中国环境科学研究院 | Technology for producing vinasse somatic protein fodder by solide aerobic fermentation |
| CN102643872A (en) * | 2012-04-01 | 2012-08-22 | 江苏谷硅新材料股份有限公司 | Method for comprehensively utilizing grains of distillers based on biotechnology |
| CN102943049A (en) * | 2012-11-23 | 2013-02-27 | 山东宏河圣齐生物工程有限公司 | Process for producing cryytococcus neoformans culture through solid fermentation |
-
2013
- 2013-07-10 CN CN201310287286.1A patent/CN104273318A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1285156A (en) * | 1999-08-23 | 2001-02-28 | 中国环境科学研究院 | Technology for producing vinasse somatic protein fodder by solide aerobic fermentation |
| CN102643872A (en) * | 2012-04-01 | 2012-08-22 | 江苏谷硅新材料股份有限公司 | Method for comprehensively utilizing grains of distillers based on biotechnology |
| CN102943049A (en) * | 2012-11-23 | 2013-02-27 | 山东宏河圣齐生物工程有限公司 | Process for producing cryytococcus neoformans culture through solid fermentation |
Non-Patent Citations (1)
| Title |
|---|
| 洪坚平等: "《应用微生物学（第2版）》", 28 February 2011, 中国林业出版社 * |
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