CN104258061A - 一种具有降血糖作用的组合物及其制备方法 - Google Patents
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Abstract
本发明公开了一种具有降血糖作用的组合物及其制备方法。由如下组分组成:西洋参10%-80%,麦冬10%-80%,黄精10%-80%。上述组合物的制备方法包括如下步骤:(1)取西洋参、麦冬和黄精,干燥,去杂,粉碎,过筛,置于超临界萃取提取罐中,以超临界CO2萃取,提取液静置待用;(2)残渣置于超高压提取罐中,加16倍体积水提取三次,合并提取液,浓缩;(3)合并超临界CO2萃取物和超高压水提物即得。本发明的组合物具有显著的降血糖效果,并且可以明显改善消渴症状。本发明采取超临界CO2萃取方法和超高压两种提取方法,超临界CO2可以充分提取脂溶性成分,超高压水提法可以提取水溶性多糖等成分,可以充分提取药材中的有效成分,发挥最大的功效作用。
Description
技术领域
本发明属于中医药保健品领域,具体涉及到西洋参、麦冬和黄精提取物的降血糖作用和提取方法及制剂方法。
背景技术
西洋参(Panax Quinqefollium L)系五加科(Araliacae)人参属(Panax)植物,其根是驰名中外的医疗保健药品。西洋参具有滋补强壮、养血生津、宁神益智的功能,我国使用西洋参已有200多年的历史。近年来国内外学者对西洋参的用途做了大量的研究,取得了许多可喜的成果,其提高免疫力作用、耐缺氧和抗疲劳及抗癌作用已有大量文献报道和专利产品,但是迄今为止,很少见其在糖尿病方面作用的文献报道和专利产品。
麦冬为百合科沿阶草属植物麦冬Ophiopogonjaponicus(L.F)Ker-Gawl的干燥块根,味甘、微苦、性微寒,归心、肺、胃经,具有养阴生津、润肺清心的功效,用于消渴、心烦失眠等。麦冬为常用的中药之一,主产地为四川和浙江。麦冬的有效部位为甾体皂苷、多糖、黄酮等,具有多种药理功能,已广泛应用于糖尿病、心血管等疾病。
黄精(rhizoma polygonti)是百合科(liliaceae)黄精属(polygonatum)多种植物的根茎,主要分布于北温带和北亚热带,我国约有31种,分布于除南方热带以外的我国广大地区,其资源蕴藏极为丰富。黄精在我们的药用历史已逾2000年,中医认为,黄精性平、味甘,入脾、肾、肺经,具有补肾益精、滋阴润燥的功效。随着现代药理研究的不断深入,现已证明黄精具有降低血糖、血脂,调节和增强免疫功能,延缓衰老,抗炎和抗病毒等重要药理作用。
发明内容
本发明的目的在于提供一种降血糖效果显著的组合物及其提取方法。
为了实现上述目的,本发明采用如下技术方案:
一种具有降血糖作用的组合物,由如下重量百分数的组分组成:西洋参10%-80%,麦冬10%-80%,黄精10%-80%。
上述组合物的制备方法,包括如下步骤:
(1)取西洋参、麦冬和黄精,干燥,去杂,粉碎,过筛,置于超临界萃取提取罐中,以超临界CO2萃取,提取液静置待用;
(2)残渣置于超高压提取罐中,加16倍体积水提取三次,合并提取液,浓缩;
(3)合并超临界CO2萃取物和超高压水提物即得。
具有降血糖作用的乳剂的制备方法,包括如下步骤:取上述混合提取物、加入聚山梨酯-80、豆油及蒸馏水,置于乳匀机中,研磨、搅匀即得。
与现有技术相比,本发明具有如下有益效果:本发明经过大量实验,充分考虑君臣佐使以及物质混配的协同作用,首次将西洋参,麦冬和黄精三者混配,并确定了其混配比例,所得组合物具有显著的降血糖效果,并且可以明显改善消渴症状。另外,本发明采取超临界CO2萃取方法和超高压两种提取方法,超临界CO2可以充分提取脂溶性成分,超高压水提法可以提取水溶性多糖等成分,合并两种提取液,可以充分提取药材中的有效成分,发挥最大的功效作用。本发明的混合提取物可以根据需要制成多种剂型,比如乳剂,用于辅助治疗糖尿病具有副作用少,不易产生耐受性等优点。
具体实施方式
实施例1:(重量百分数)
取西洋参10%,麦冬占40%和黄精占50%,干燥,去杂,粉碎,过筛,置于超临界萃取提取罐中,以超临界CO2萃取,提取液静置待用;残渣置于超高压提取罐中,加16倍体积水提取三次,每次5分钟,合并提取液,浓缩;合并超临界CO2萃取物和超高压水提物即得。实施例2:(重量百分数)
取西洋参40%,麦冬占40%和黄精占20%,干燥,去杂,粉碎,过筛,置于超临界萃取提取罐中,以超临界CO2萃取,提取液静置待用;残渣置于超高压提取罐中,加16倍体积水提取三次,每次5分钟,合并提取液,浓缩;合并超临界CO2萃取物和超高压水提物即得。实施例3:(重量百分数)
取西洋参60%,麦冬占30%和黄精占10%,干燥,去杂,粉碎,过筛,置于超临界萃取提取罐中,以超临界CO2萃取,提取液静置待用;残渣置于超高压提取罐中,加16倍体积水提取三次,每次5分钟,合并提取液,浓缩;合并超临界CO2萃取物和超高压水提物即得。实施例4:取实施例1的提取物进行降血糖实验,结果表明该提取物对四氧嘧啶诱导的糖尿病大鼠的血糖具有降低作用。具体方法如下:(1)实验动物与糖尿病大鼠模型的制备:实验大鼠在室内温度为20-25度,相对湿度为50-70%的环境中,普通饲料喂养,适应新环境一个月后编号,依次取眼底静脉血、葡萄糖氧化酶法测空腹血糖值。然后随机抽取60只大鼠禁食12小时,以每只按0.4g/Kg新鲜配制的四氧嘧啶生理盐水溶液注入大鼠腹腔皮下,造成实验性糖尿病模型。恢复正常饮食后两天切尾,快速血糖仪测其全血血糖,筛选血糖>11.1mmol/L的大鼠。再观察60只造模大鼠1个月,结果共有50只纳入实验程序。
(2)血糖测定:造模成功后的第40天,取50只造模成功大鼠,分随机分成5组:二甲双胍对照组,模型对照组,治疗组1、2、3(本提取物50mg/kg、75mg/kg、100mg/kg),每组10只。以及正常对照大鼠10只,共60只大鼠眼底静脉取血,用氧化酶法测血糖,记录其值后,喂服60只大鼠每日基础饲料外,二甲双胍组每只每日灌服二甲双胍100mg/kg,1天食尽,本产品三个剂量组大鼠每日每次分别灌服本提取物50mg/kg/d、75mg/kg/d、100mg/kg/d,正常对照及模型对照组灌服等体积生理盐水,以上连续灌服15天,第16天禁食12小时,再取实验大鼠的眼底静脉血测血糖值。
(3)结果分析:
表1.提取物对四氧嘧啶糖尿病小鼠血糖的影响
与正常对照组比较,△△△:P<0.001。与模型对照组比较,**:P<0.01,***:P<0.001。
给药前,各治疗组与模型对照组小鼠血糖含量无明显差异(P>0.05),与正常对照组相比则有极显著性差异(P<0.001)。给药后,提取物治疗组大鼠血糖含量明显低于给药前,同时亦明显低于模型对照组(P<0.01)。说明本组合物的提取物具有明显地降血糖效果。
实施例5:参照实施例4,将实施例1替换为实施例2,其他不变,结果说明实施例2的组合物也具有显著的降血糖效果。
实施例6:参照实施例4,将实施例1替换为实施例3,其他不变,结果说明实施例3的组合物也具有显著的降血糖效果。
Claims (4)
1.一种具有降血糖作用的组合物,其特征在于,由如下重量百分数的组分组成:西洋参10%-80%,麦冬10%-80%,黄精10%-80%。
2.权利要求1所述组合物的制备方法,其特征在于包括如下步骤:
(1)取西洋参、麦冬和黄精,干燥,去杂,粉碎,过筛,置于超临界萃取提取罐中,以超临界CO2萃取,提取液静置待用;
(2)残渣置于超高压提取罐中,加16倍体积水提取三次,合并提取液,浓缩;
(3)合并超临界CO2萃取物和超高压水提物即得。
3.一种具有降血糖作用的乳剂的制备方法,其特征在于包括如下步骤:取权利要求2的混合提取物、加入聚山梨酯-80、豆油及蒸馏水,置于乳匀机中,研磨、搅匀即得。
4.权利要求1所述组合物在制备用于改善消渴症状药物中的应用。
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CN115227791A (zh) * | 2022-06-27 | 2022-10-25 | 中保健康科技(广东)有限公司 | 一种具有降血糖作用的组合物及其制备方法 |
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