CN104256056B - Utilize animal feed prepared by glutamic acid fermentation waste material - Google Patents
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Abstract
The invention belongs to amino acid fermentation field of waste liquid treatment, disclose a kind of animal feed utilizing glutamic acid fermentation waste material to prepare, it is prepared as follows and forms: step 1) filtering fermentation liquor, step 2) decolour, concentrate and crystallize, step 3) hydrolyzes, the compound medicated powder of step 4) preparation, step 5) prepares major ingredient, and step 6) prepares feedstuff.Feedstuff prepared by the present invention is effectively utilized amino acid fermentation waste material, reduces Feed Manufacturing cost, it is achieved that turn waste into wealth, and adds without antibiont and hormone, it is ensured that the edible safety of meat products.
Description
Technical field
The invention belongs to biofermentation industry Wastewater treating field, particularly relate to animal feed prepared by glutamic acid fermentation waste material.
Background technology
Glutamic acid, is a kind of acidic amino acid.Intramolecular contains two carboxyls, and chemical name is alpha-amido 1,3-propanedicarboxylic acid.Glutamic acid is that inner Suo Xun finds for 1856, and for clear crystal, having delicate flavour, be present in a large number in grain protein, in animal brain, content is the most more.Account for critical role during glutamic acid protein metabolism in vivo, participate in the important chemical reaction of many in animal, plant and microorganism.Sodium glutamate is commonly called as monosodium glutamate, is important tasty agents, fragrance is had potentiation.Sodium glutamate is widely used in food flavor, both can be used alone, again can be with other aminoacid etc. using.In food, there is flavouring effect.Development along with amino acid fermentation technology, China has become as the production and consumption big country of monosodium glutamate, but the wastewater flow rate discharged during glutamate production is big, fermented gournet powder liquid mother solution of discharge after waiting electricity to extract glutamic acid has that CODCr is high, BOD5 is high, thalline content is high, content is high for sulfate radical (using sulphuric acid instead to adjust before pH as chloride ion), ammonia-nitrogen content height and the feature of pH value low " five high low ".It it is the industrial wastewater that a kind of difficulty of governance is the biggest.Owing to can not effectively administer gourmet powder waste water, many Gourmet Powder Factories are put into the row of major polluting sources, whole nation unit, and the improvement of gourmet powder waste water has become as the great difficult problem of restriction glutamate production enterprise development.The a large amount of thalline contained in waste water, it is a kind of single cell protein, containing rich in protein.
In recent years, livestock feed price rises steadily, and the benefit making raiser is year after year low, causes aquaculture very risky, has dampened the enthusiasm of numerous raisers significantly.Aquaculture cost the productivity effect of each raiser always in left and right, and it returns root to be that feed cost is too high eventually.Modern cultivates, casting aside traditional raising the most completely is that to use coarse grain, grain offal, the rotten dish of grass be main animal diets, and support isotype slowly, it is to fully rely on full price commercial feed to carry out throwing something and feeding and carry out quickly raising, substantial amounts of consumables feedstuff in breeding process the most mostly.So, the height of feed cost determines the good with bad of benefit completely with low.Current full price commercial feed be mostly with concentrate be main be equipped with various auxin again, hormone is formed by combining.The drug residue such as antibiotic, growth hormone in pig feed so that quality and the mouthfeel of Carnis Sus domestica significantly decline, can not meet again people's requirement to healthy diet.
In prior art CN103224454A " separation-extraction technology of a kind of glutamic acid ", utilize tropina to be prepared for feedstuff, but this feed product is not isolated tropina and processed, and digests and assimilates effect bad, and resistance against diseases is not enough；And create substantial amounts of mother solution and need to discharge, do not accomplish the purpose of energy-saving and emission-reduction.How developing the feedstuff that a kind of with low cost and antibiotic-free hormonal medicaments adds is prior art technical problem urgently to be resolved hurrily.
Summary of the invention
In order to overcome the defect of prior art, the invention provides a kind of animal feed utilizing glutamic acid fermentation waste material to prepare, this feedstuff is effectively utilized amino acid fermentation waste material, reduce Feed Manufacturing cost, achieve and turn waste into wealth, and nonreactive bio-hormone adds, it is ensured that the edible safety of meat products.
For achieving the above object, the present invention is by the following technical solutions:
A kind of animal feed utilizing glutamic acid fermentation waste material to prepare, it is prepared as follows and forms: step 1) filtering fermentation liquor, step 2) decolour, concentrate and crystallize, step 3) hydrolyzes, the compound medicated powder of step 4) preparation, step 5) prepares major ingredient, and step 6) prepares feedstuff.
Specifically, described feedstuff is prepared as follows and forms:
Step 1) filtering fermentation liquor: glutami acid fermentation liquor is degerming through micro-filtrate membrane filtration, collects trapped substance and filtered solution respectively；Then filtered solution is proceeded ultrafiltration membrance filter, collects concentrated solution and penetrating fluid；Above-mentioned trapped substance and concentrated solution are merged into feed liquid A standby；
Step 2) decolour, concentrate and crystallize: step (1) gained penetrating fluid pumps into bleacher and carries out desolventing technology, bleacher adds the activated carbon of penetrating fluid quality 0.5%, the temperature controlled in bleacher is 45 DEG C, after decolouring 30min, plate-and-frame filtration removes activated carbon, then concentrate, crystallization, finally centrifugal acquisition glutamic acid crystal and mother solution；
Step 3) hydrolyzes: by step 2) drying of gained feed liquid A, pulverizer is ground into powder, it is subsequently placed in reactor, add the hydrochloric acid of 6-8mol/L, not have raw material to be as the criterion, stirring hydrolysis 12-16 hour at a temperature of 70 DEG C, mixing speed is 100 turns/min, then heating volatilizes HCl gas to the pH of solution is 2-3, and finally adding sodium hydrate regulator solution is 6-7 to pH, obtains Freamine Ⅲ；
The compound medicated powder of step 4) preparation:
A, weigh the Radix Astragali 10 parts, Flos Lonicerae 8 parts, Herba Taraxaci 6 parts, Radix Bupleuri 5 parts, 3 parts of Radix Glycyrrhizae, Flos Campsis 2 parts and the Rhizoma Anemarrhenae 1 part according to weight portion, standby；
B, taking the Radix Astragali, Flos Lonicerae, Herba Taraxaci and Radix Bupleuri, mix homogeneously, the water intense fire adding 3 times of weight boils, and then slow fire boiling 30 minutes, are collected by filtration filtrate, is condensed into the extractum that density is 1.2g/ml；After 70 DEG C dry, it is ground into powder, is complex 1；
C, extracting liquorice, Flos Campsis and the Rhizoma Anemarrhenae, mix homogeneously, it is heated to reflux 1 hour with 80% ethanol (v/v), filtrate, filtrate evaporative removal ethanol are recovered by filtration, then concentrating and obtaining density is 1.1-1.2g/ml condensed cream, and crushed after being dried obtains complex 2；
D, by complex 1 and complex 2 mix homogeneously, obtain compound medicated powder；
Step 5) prepares major ingredient: Testa Tritici, bean cake, Sorghum vulgare Pers., Pericarppium arachidis hypogaeae and corn straw are mixed, and pulverizes with pulverizer, crosses 100 mesh sieves, and then 500 turns/min stirs 10min, is subsequently added step 2) mother liquid obtained, 100 turns/min stirs 24 hours, obtains major ingredient；
Step 6) prepares feedstuff: adds in major ingredient by compound medicated powder and Freamine Ⅲ, heats while stirring, stops heating, granulator granulation, dry, pack and get final product when being 30-40% to moisture.
Preferably, in described step 5), the weight ratio of Testa Tritici, bean cake, Sorghum vulgare Pers., Pericarppium arachidis hypogaeae, corn straw and mother solution is 5-8:3-4:1-2:1-2:1-2:10-15.In described step 6), the weight ratio of compound medicated powder, Freamine Ⅲ and major ingredient is 1-2:30-45:350-500.Described animal can be pig.
The beneficial effect that the present invention obtains specifically includes that
Feedstuff prepared by the present invention make use of glutamic acid fermentation waste material dexterously, reduces Feed Manufacturing cost, it is achieved that turn waste into wealth, and nonreactive bio-hormone adds, it is ensured that the edible safety of meat products；Glutamic acid fermentation technique of the present invention eliminates mother liquid disposal process, reduces production cost, it is achieved that turn waste into wealth, the utilization rate of resource is greatly improved；The tropina being difficult to digest is hydrolyzed to amino acid profiles by the present invention, and supplements salinity dexterously, and nutrition is more comprehensive, improves the appetite of letting animals feed, strengthens digestion, enhances metabolism；The present invention avoids using antibiotic and hormone, with the addition of natural Chinese medicine component, uses different extracting method to obtain its effective ingredient to greatest extent, and compatibility is reasonable, possesses heat-clearing and toxic substances removing, improves immunity and prevents and treats effect of pestilence.
Detailed description of the invention
For the technical scheme making those skilled in the art be more fully understood that in the application, below in conjunction with the application specific embodiment, the present invention is more clearly and completely described, it is clear that, described embodiment is only some embodiments of the present application rather than whole embodiments.Based on the embodiment in the application, the every other embodiment that those of ordinary skill in the art are obtained under not making creative work premise, all should belong to the scope of protection of the invention.
Embodiment 1
A kind of animal feed utilizing glutamic acid fermentation waste material to prepare, it is prepared as follows and forms:
Step 1) filtering fermentation liquor: glutami acid fermentation liquor is degerming through micro-filtrate membrane filtration, collects trapped substance and filtered solution respectively；Then filtered solution is proceeded ultrafiltration membrance filter, collects concentrated solution and penetrating fluid；Above-mentioned trapped substance and concentrated solution are merged into feed liquid A standby；Wherein, micro-filtration membrane is inorganic ceramic film, and molecular cut off is 2000MW, and microfiltration temperature is 40 DEG C；Ultrafilter membrane molecular cut off is 300MW, and ultrafiltrate temperature is 40 DEG C；
Step 2) decolour, concentrate and crystallize: step (1) gained penetrating fluid pumps into bleacher and carries out desolventing technology, bleacher adds the activated carbon accounting for penetrating fluid quality 0.5%, the temperature controlled in bleacher is 45 DEG C, after decolouring 30min, plate-and-frame filtration removes activated carbon, then concentrate, crystallization, finally centrifugal acquisition glutamic acid crystal and mother solution；Described concentration parameter is: temperature 60 C, and vacuum is-0.1kpa；
Step 3) hydrolyzes: feed liquid A dried, pulverizer is ground into powder, it is subsequently placed in reactor, add the hydrochloric acid of 6mol/L, not have raw material to be as the criterion, stirring hydrolysis 16 hours at a temperature of 70 DEG C, mixing speed is 100 turns/min, then heating volatilizes HCl gas to the pH of solution is 2, and finally adding sodium hydrate regulator solution is 6 to pH, obtains Freamine Ⅲ；
The compound medicated powder of step 4) preparation:
A, weigh the Radix Astragali 10 parts, Flos Lonicerae 8 parts, Herba Taraxaci 6 parts, Radix Bupleuri 5 parts, 3 parts of Radix Glycyrrhizae, Flos Campsis 2 parts and the Rhizoma Anemarrhenae 1 part respectively according to weight portion, standby；
B, taking the Radix Astragali, Flos Lonicerae, Herba Taraxaci and Radix Bupleuri, mix homogeneously, the water intense fire adding 3 times of weight boils, and then slow fire boiling 30 minutes, are collected by filtration filtrate, is condensed into the extractum that density is 1.2g/ml；After 70 DEG C dry, it is ground into powder, is complex 1；
C, extracting liquorice, Flos Campsis and the Rhizoma Anemarrhenae, mix homogeneously, with 80% alcohol heating reflux 1 hour, filtrate, filtrate evaporative removal ethanol are recovered by filtration, then concentrating and obtaining density is 1.1g/ml condensed cream, and crushed after being dried obtains complex 2；
D, by complex 1 and complex 2 mix homogeneously, obtain compound medicated powder；
Step 5) prepares major ingredient:
Testa Tritici, bean cake, Sorghum vulgare Pers., Pericarppium arachidis hypogaeae and corn straw being mixed, pulverize with pulverizer, cross 100 mesh sieves, then 500 turns/min stirs 10min, is subsequently added step 2) mother liquid obtained, 100 turns/min stirs 24 hours, obtains major ingredient；Wherein, the weight ratio of Testa Tritici, bean cake, Sorghum vulgare Pers., Pericarppium arachidis hypogaeae, corn straw and mother solution is 5:3:1:1:1:10；
Step 6) prepares feedstuff: adds in major ingredient by compound medicated powder and Freamine Ⅲ, heats while stirring, is 30%(w/w to moisture) time stop heating, granulator granulation, dry, pack and get final product；Wherein, the weight ratio of compound medicated powder, Freamine Ⅲ and major ingredient is 1:30:350.
Embodiment 2
A kind of animal feed utilizing glutamic acid fermentation waste material to prepare, it is prepared as follows and forms:
Step 1) filtering fermentation liquor: glutami acid fermentation liquor is degerming through micro-filtrate membrane filtration, collects trapped substance and filtered solution respectively；Then filtered solution is proceeded ultrafiltration membrance filter, collects concentrated solution and penetrating fluid；Above-mentioned trapped substance and concentrated solution are merged into feed liquid A standby；Wherein, micro-filtration membrane is inorganic ceramic film, and molecular cut off is 2000MW, and microfiltration temperature is 40 DEG C；Ultrafilter membrane molecular cut off is 300MW, and ultrafiltrate temperature is 40 DEG C；
Step 2) decolour, concentrate and crystallize: step (1) gained penetrating fluid pumps into bleacher and carries out desolventing technology, bleacher adds the activated carbon of penetrating fluid quality 0.5%, the temperature controlled in bleacher is 45 DEG C, after decolouring 30min, plate-and-frame filtration removes activated carbon, then concentrate, crystallization, finally centrifugal acquisition glutamic acid crystal and mother solution；Described concentration parameter is: temperature 60 C, and vacuum is-0.1kpa；
Step 3) hydrolyzes: feed liquid A dried, pulverizer is ground into powder, it is subsequently placed in reactor, add the hydrochloric acid of 8mol/L, not have raw material to be as the criterion, stirring hydrolysis 12 hours at a temperature of 70 DEG C, mixing speed is 100 turns/min, then heating volatilizes HCl gas to the pH of solution is 3, and finally adding sodium hydrate regulator solution is 7 to pH, obtains Freamine Ⅲ；
The compound medicated powder of step 4) preparation:
A, weigh the Radix Astragali 10 parts, Flos Lonicerae 8 parts, Herba Taraxaci 6 parts, Radix Bupleuri 5 parts, 3 parts of Radix Glycyrrhizae, Flos Campsis 2 parts and the Rhizoma Anemarrhenae 1 part according to weight portion, standby；
B, taking the Radix Astragali, Flos Lonicerae, Herba Taraxaci and Radix Bupleuri, mix homogeneously, the water intense fire adding 3 times of weight boils, and then slow fire boiling 30 minutes, are collected by filtration filtrate, is condensed into the extractum that density is 1.2g/ml；After 70 DEG C dry, it is ground into powder, is complex 1；
C, extracting liquorice, Flos Campsis and the Rhizoma Anemarrhenae, mix homogeneously, with 80% alcohol heating reflux 1 hour, filtrate, filtrate evaporative removal ethanol are recovered by filtration, then concentrating and obtaining density is 1.2g/ml condensed cream, and crushed after being dried obtains complex 2；
D, by complex 1 and complex 2 mix homogeneously, obtain compound medicated powder；
Step 5) prepares major ingredient:
Testa Tritici, bean cake, Sorghum vulgare Pers., Pericarppium arachidis hypogaeae and corn straw being mixed, pulverize with pulverizer, cross 100 mesh sieves, then 500 turns/min stirs 10min, is subsequently added step 2) mother liquid obtained, 100 turns/min stirs 24 hours, obtains major ingredient；Wherein, the weight ratio of Testa Tritici, bean cake, Sorghum vulgare Pers., Pericarppium arachidis hypogaeae, corn straw and mother solution is 5-8:3-4:1-2:1-2:1-2:10-15；
Step 6) prepares feedstuff: adds in major ingredient by compound medicated powder and Freamine Ⅲ, heats while stirring, is 40%(w/w to moisture) time stop heating, granulator granulation, dry, pack and get final product；Wherein, the weight ratio of compound medicated powder, Freamine Ⅲ and major ingredient is 2:45:500.
Embodiment 3
Feed efficiency test prepared by the present invention:
One, choosing 150 pigs infecting swine fevers, pig age is between the 2-4 month；Symptoms is: innominate high fever, loss of appetite or disappearance, occurs eye conjunctivitis also to have purulent secretion, and nasal cavity permanent current goes out mycopus, has vomiting, has blood in stool；Skin inside the basal part of the ear, abdominal part, stock often has many petechial hemorrhages or bright redder point.Mesenteric adenophyma is swollen, hemorrhagic enteritis often occurs, with the button-type ulcer in colorectal mucosa.It is randomly divided into three groups, often group 50；Three groups all use antipyretic and antitoxic powder treatment, and every 1kg body weight is taken 20g orally, is used in conjunction 7 days.
Experimental group: give the feedstuff of the embodiment of the present invention 1 preparation；Matched group 1: give big swine feed in logical prestige；Matched group 2: give embodiment 1 feedstuff, without Chinese medicine component.
Efficacy assessment standard: cure: symptom is wholly absent, and main physico-chemical examination index is normal；Take a turn for the better: each side symptom makes moderate progress compared with before treatment；Without positive effect: each side symptom is all not improved compared with before treatment.Concrete case statistics is shown in Table 1:
Table 1
| Group | Number of cases | Cure | Take a turn for the better | Without positive effect | Obvious effective rate |
| Experimental group | 50 | 25 | 18 | 7 | 86% |
| Matched group 1 | 50 | 13 | 17 | 20 | 60% |
| Matched group 2 | 50 | 15 | 16 | 19 | 62% |
Two, choose the piglet 200 of 60 days, be divided into two groups, often group 100, the diet that wherein experimental group is prepared with the present embodiment 1, matched group hog grower feed in logical prestige；Other rearing conditions two group is identical.Detect indices after raising 9 weeks and see table 2.
Table 2
| Index | Experimental group | Matched group |
| Test initial individuals weight (kg) | 21.80 | 22.30 |
| Test terminates individual weight (kg) | 70.50 | 68.04 |
| Daily gain (kg) | 0.773 | 0.726 |
| Head all consumes material (kg) | 113 | 115 |
| Feed-weight ratio | 2.32 | 2.51 |
Conclusion: diet effect prepared by the present invention is preferable, and daily gain and feed-weight ratio are better than market and commonly use pig feed, and cost is cheap compared with commercial diet.
Listed above is only the optimal specific embodiment of the present invention.It is clear that the invention is not restricted to above example, it is also possible to there are many deformation.All deformation that those of ordinary skill in the art can directly derive from present disclosure or associate, are all considered as protection scope of the present invention.
Claims (1)
1. one kind utilizes animal feed prepared by glutamic acid fermentation waste material, it is characterised in that described feedstuff is prepared as follows and forms:
Step 1) filtering fermentation liquor: glutami acid fermentation liquor is degerming through micro-filtrate membrane filtration, collects trapped substance and filtered solution respectively；Then filtered solution is proceeded ultrafiltration membrance filter, collects concentrated solution and penetrating fluid；Above-mentioned trapped substance and concentrated solution are merged into feed liquid A standby；
Step 2) decolour, concentrate and crystallize: step 1) gained penetrating fluid pumps into bleacher and carries out desolventing technology, bleacher adds the activated carbon accounting for penetrating fluid quality 0.5%, the temperature controlled in bleacher is 45 DEG C, after decolouring 30min, plate-and-frame filtration removes activated carbon, then concentrate, crystallization, finally centrifugal acquisition glutamic acid crystal and mother solution；
Step 3) hydrolyzes: step 1) gained feed liquid A dried, pulverizer is ground into powder, it is subsequently placed in reactor, add the hydrochloric acid of 6-8mol/L, not have raw material to be as the criterion, stirring hydrolysis 12-16 hour at a temperature of 70 DEG C, mixing speed is 100 turns/min, then heating volatilizes HCl gas to the pH of solution is 2-3, and finally adding sodium hydrate regulator solution is 6-7 to pH, obtains Freamine Ⅲ；
The compound medicated powder of step 4) preparation:
A, weigh the Radix Astragali 10 parts, Flos Lonicerae 8 parts, Herba Taraxaci 6 parts, Radix Bupleuri 5 parts, 3 parts of Radix Glycyrrhizae, Flos Campsis 2 parts and the Rhizoma Anemarrhenae 1 part according to weight portion, standby；
B, taking the Radix Astragali, Flos Lonicerae, Herba Taraxaci and Radix Bupleuri, mix homogeneously, the water intense fire adding 3 times of weight boils, and then slow fire boiling 30 minutes, are collected by filtration filtrate, is condensed into the extractum that density is 1.2g/ml；After 70 DEG C dry, it is ground into powder, is complex 1；
C, extracting liquorice, Flos Campsis and the Rhizoma Anemarrhenae, mix homogeneously, with 80% alcohol heating reflux 1 hour, filtrate, filtrate evaporative removal ethanol are recovered by filtration, then concentrating and obtaining density is 1.1-1.2g/ml condensed cream, and crushed after being dried obtains complex 2；
D, by complex 1 and complex 2 mix homogeneously, obtain compound medicated powder；
Step 5) prepares major ingredient: Testa Tritici, bean cake, Sorghum vulgare Pers., Pericarppium arachidis hypogaeae and corn straw are mixed, and pulverizes with pulverizer, crosses 100 mesh sieves, and then 500 turns/min stirs 10min, is subsequently added step 2) mother liquid obtained, 100 turns/min stirs 24 hours, obtains major ingredient；
Step 6) prepares feedstuff: adds in major ingredient by compound medicated powder and Freamine Ⅲ, heats while stirring, stops heating, granulator granulation, dry, pack and get final product when being 30-40% to moisture；
In described step 5), the weight ratio of Testa Tritici, bean cake, Sorghum vulgare Pers., Pericarppium arachidis hypogaeae, corn straw and mother solution is 5-8:3-4:1-2:1-2:1-2:10-15；
In described step 6), the weight ratio of compound medicated powder, Freamine Ⅲ and major ingredient is 1-2:30-45:350-500.
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| CN106819398A (en) * | 2016-12-27 | 2017-06-13 | 新昌县拜瑞珂生物技术有限公司 | The animal feed prepared using glutamic acid fermentation waste material |
| CN106946976A (en) * | 2017-05-19 | 2017-07-14 | 卢松 | Recycle the method that glutamic acid fermentation discards thalline |
| CN109275782A (en) * | 2018-05-18 | 2019-01-29 | 山阳县恒桓生物科技有限公司 | A kind of processing method of waste water of mgs |
| CN111513193A (en) * | 2020-05-11 | 2020-08-11 | 南通励成生物工程有限公司 | Animal feed and preparation method thereof |
| CN112772780A (en) * | 2020-12-27 | 2021-05-11 | 新疆阜丰生物科技有限公司 | Hydrolysis method of amino acid fermentation waste and application of amino acid fermentation waste in feed additive |
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| CN101168515B (en) * | 2007-11-15 | 2010-08-25 | 山东阜丰生物科技开发有限公司 | Technique for utilizing glutamic acid production waste liquid |
| CN101671705A (en) * | 2009-07-23 | 2010-03-17 | 阜新豪森生物科技有限公司 | Extraction technology of glutamic acid recovered by combining crystal transformation and ion exchange |
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