CN104237442A - 试剂盒及其应用以及联合检测多种氨基酸和肉毒碱的方法 - Google Patents
试剂盒及其应用以及联合检测多种氨基酸和肉毒碱的方法 Download PDFInfo
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Abstract
Description
氨基酸(AA) | 浓度 | 肉毒碱(AC) | 浓度 |
2H4-Ala | 2.50μmol/L | 2H9-C0 | 0.76μmol/L |
2H4,13C-Arg | 2.50μmol/L | 2H3-C2 | 0.19μmol/L |
2H2-Cit | 2.50μmol/L | 2H3-C3 | 0.038μmol/L |
15N,2-13C-Gly | 12.50μmol/L | 2H3-C4 | 0.038μmol/L |
2H3-Leu | 2.50μmol/L | 2H9-C5 | 0.038μmol/L |
2H3-Met | 2.50μmol/L | 2H3-C8 | 0.038μmol/L |
2H2-Orn | 2.50μmol/L | 2H9-C14 | 0.038μmol/L |
2H6-Phe | 2.50μmol/L | 2H3-C16 | 0.076μmol/L |
13C6-Tyr | 2.50μmol/L | ||
2H8-Val | 2.50μmol/L |
平均值 | SD | CV% | 靶值 | 范围上限 | 下限 | |
VAL | 326.77 | 47.52 | 14.54 | 312.00 | 447.00 | 177.00 |
TYR | 305.75 | 23.56 | 7.70 | 287.00 | 382.29 | 209.00 |
PHE | 209.33 | 16.31 | 7.79 | 198.00 | 271.95 | 144.00 |
MET | 99.53 | 7.28 | 7.32 | 103.00 | 146.36 | 58.00 |
LEU | 477.49 | 38.64 | 8.09 | 425.00 | 600.63 | 305.00 |
GLY | 574.01 | 40.73 | 7.09 | 526.00 | 695.07 | 412.00 |
CIT | 165.61 | 11.50 | 6.94 | 168.00 | 217.02 | 120.00 |
ALA | 558.26 | 57.55 | 10.31 | 603.00 | 733.33 | 399.00 |
C8 | 0.55 | 0.05 | 9.60 | 0.60 | 0.91 | 0.30 |
C6 | 0.55 | 0.04 | 8.06 | 0.65 | 0.90 | 0.35 |
C5 | 1.05 | 0.15 | 14.36 | 1.20 | 1.60 | 0.60 |
C4 | 2.82 | 0.28 | 10.01 | 3.10 | 3.02 | 2.80 |
C3 | 9.52 | 0.74 | 7.79 | 10.50 | 13.76 | 6.00 |
C2 | 39.27 | 3.03 | 7.72 | 42.30 | 45.48 | 34.80 |
C10 | 0.44 | 0.04 | 8.87 | 0.56 | 0.61 | 0.41 |
C0 | 166.67 | 18.95 | 11.37 | 182.00 | 221.46 | 125.00 |
C5DC | 0.55 | 0.06 | 11.64 | 0.68 | 0.71 | 0.53 |
C18 | 2.36 | 0.20 | 8.38 | 2.55 | 3.07 | 1.95 |
C16 | 11.80 | 0.96 | 8.14 | 12.50 | 12.85 | 12.20 |
C14 | 1.77 | 0.15 | 8.28 | 1.90 | 2.20 | 1.60 |
C12 | 1.99 | 0.21 | 10.75 | 2.20 | 2.67 | 1.60 |
Claims (11)
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Cited By (5)
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CN106353424A (zh) * | 2016-08-31 | 2017-01-25 | 武汉生物技术研究院管理有限责任公司 | 一种临床样本中游离氨基酸的定量检测方法 |
CN107621500A (zh) * | 2016-07-14 | 2018-01-23 | 上海可力梅塔生物医药科技有限公司 | 氨基酸与肉碱串联质谱衍生化检测法 |
CN108152380A (zh) * | 2016-12-05 | 2018-06-12 | 山东国际生物科技园发展有限公司 | 串联质谱非衍生化法多种氨基酸和肉碱检测试剂盒 |
CN108645924A (zh) * | 2017-06-22 | 2018-10-12 | 南方医科大学 | 基于超高效液相色谱串联质谱技术的新生儿代谢物的检测方法 |
CN110118845A (zh) * | 2018-02-06 | 2019-08-13 | 北京金则医学科技发展有限公司 | 检测多种氨基酸和酰基肉碱、甲基丙二酸的方法和试剂盒 |
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Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107621500A (zh) * | 2016-07-14 | 2018-01-23 | 上海可力梅塔生物医药科技有限公司 | 氨基酸与肉碱串联质谱衍生化检测法 |
CN106353424A (zh) * | 2016-08-31 | 2017-01-25 | 武汉生物技术研究院管理有限责任公司 | 一种临床样本中游离氨基酸的定量检测方法 |
CN108152380A (zh) * | 2016-12-05 | 2018-06-12 | 山东国际生物科技园发展有限公司 | 串联质谱非衍生化法多种氨基酸和肉碱检测试剂盒 |
CN108645924A (zh) * | 2017-06-22 | 2018-10-12 | 南方医科大学 | 基于超高效液相色谱串联质谱技术的新生儿代谢物的检测方法 |
CN108645924B (zh) * | 2017-06-22 | 2021-07-20 | 南方医科大学 | 基于超高效液相色谱串联质谱技术的新生儿代谢物的检测方法 |
CN110118845A (zh) * | 2018-02-06 | 2019-08-13 | 北京金则医学科技发展有限公司 | 检测多种氨基酸和酰基肉碱、甲基丙二酸的方法和试剂盒 |
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Address after: 510000 No. 6, Yinyi street, economic and Technological Development Zone, Guangzhou, Guangdong Patentee after: Guangzhou Fenghua Biological Co.,Ltd. Address before: No.6, Yinyi street, economic and Technological Development Zone, Guangzhou, Guangdong 510730 Patentee before: GUANGZHOU FENGHUA BIOENGINEERING Co.,Ltd. |