CN104224852A - Efficient composite micro-ecological preparation and preparation method thereof - Google Patents

Abstract

The invention relates to an efficient composite micro-ecological preparation and a preparation method thereof. According to the efficient composite micro-ecological preparation, bifidobacteria and lactobacillus are fermented by a mixed fermentation mode of deep liquid fermentation and solid fermentation; stachyose is added into a liquid fermentation culture medium; the stachyose is a natural tetrose and is functional oligose capable of remarkably promoting proliferation of beneficial bacteria such as bifidobacteria and lactobacillus; meanwhile, the stachyose has the function of conditioning gastrointestinal tracts of animals.

Description

Microbial ecological agent of a kind of composite highly effective and preparation method thereof

Technical field

The invention belongs to microbial technique application.

Background technology

Microbial ecological agent have safe and efficient, without residual hazard, without advantages such as Drug resistance, fully show and utilize Tiny ecosystem to keep healthy the superiority of diseases prevention technology.

Summary of the invention

The object of this invention is to provide microbial ecological agent of a kind of composite highly effective and preparation method thereof, this microbial ecological agent can the intestinal of mediator, and intestinal is kept fit.

Technical scheme

Microbial ecological agent of a kind of composite highly effective of the present invention and preparation method thereof, its step is as follows:

(1) bacillus bifidus activated and lactobacillus be inoculated in culture medium by the inoculum concentration of 5%-10% and carry out shaking table cultivation, this culture medium is: Semen Maydis powder 15-20g/l, bean cake powder 3-5g/l, stachyose 1-3g/l, sodium chloride 5-10g/l, PH=7.0-7.2, temperature are 35-37 degree, quiescent culture 24-48 hour;

(2) cultured for step 1 bacterium liquid is carried out centrifugalize, then add the sorbester p18 of 1% ~ 10% and the Tween 80 of 1% ~ 10%, namely the post-drying that stirs obtains the microbial ecological agent of composite highly effective.

Advantage of the present invention: the present invention adds stachyose in the medium, threose is naturally occurring a kind of tetrose, be a kind of functional oligose that significantly can promote the probiotics propagation such as bacillus bifidus, stachyose has extremely significantly proliferation function to profitable strains such as the bacillus bifidus in human gastrointestinal tract, lactobacilluss.

Detailed description of the invention:

Embodiment 1:

(1) by the bacillus bifidus activated and lactobacillus by 5% inoculum concentration be inoculated in culture medium and carry out shaking table cultivation, this culture medium is: Semen Maydis powder 15g/l, bean cake powder 5g/l, stachyose 3g/l, sodium chloride 5g/l, PH=7.0, temperature are 35 degree, quiescent culture 24 hours;

(2) cultured for step 1 bacterium liquid is carried out centrifugalize, then add the sorbester p18 of 1% and the Tween 80 of 1%, namely the post-drying that stirs obtains the microbial ecological agent of composite highly effective.

Claims (2)

1. the microbial ecological agent and preparation method thereof of a composite highly effective, is characterized in that: add stachyose in culture medium.

2. the microbial ecological agent and preparation method thereof of a kind of composite highly effective according to claim 1, is characterized in that following steps:

(1) bacillus bifidus activated and lactobacillus be inoculated in culture medium by the inoculum concentration of 5%-10% and carry out shaking table cultivation, this culture medium is: Semen Maydis powder 15-20g/l, bean cake powder 3-5g/l, stachyose 1-3g/l, sodium chloride 5-10g/l, PH=7.0-7.2, temperature are 35-37 degree, quiescent culture 24-48 hour;

(2) cultured for step 1 bacterium liquid is carried out centrifugalize, then add the sorbester p18 of 1% ~ 10% and the Tween 80 of 1% ~ 10%, namely the post-drying that stirs obtains the microbial ecological agent of composite highly effective.