CN106309505A - Microecologics and preparing method thereof - Google Patents
Microecologics and preparing method thereof Download PDFInfo
- Publication number
- CN106309505A CN106309505A CN201610718899.XA CN201610718899A CN106309505A CN 106309505 A CN106309505 A CN 106309505A CN 201610718899 A CN201610718899 A CN 201610718899A CN 106309505 A CN106309505 A CN 106309505A
- Authority
- CN
- China
- Prior art keywords
- culture medium
- lactobacillus
- bacillus bifidus
- volume ratio
- microbial ecological
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/66—Microorganisms or materials therefrom
- A61K35/74—Bacteria
- A61K35/741—Probiotics
- A61K35/744—Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs
- A61K35/745—Bifidobacteria
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/66—Microorganisms or materials therefrom
- A61K35/74—Bacteria
- A61K35/741—Probiotics
- A61K35/744—Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs
- A61K35/747—Lactobacilli, e.g. L. acidophilus or L. brevis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Chemical & Material Sciences (AREA)
- Mycology (AREA)
- Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Veterinary Medicine (AREA)
- Zoology (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Epidemiology (AREA)
- Pharmacology & Pharmacy (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Tropical Medicine & Parasitology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Virology (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention discloses microecologics and a preparing method thereof. The microecologics is prepared by the following culture medium and strain: the the culture medium is prepared from,100kg of mineral water, 40kg of soybean milk, 30kg of beef soup, 3kg of glucose and 2kg of vitamin C. The strain is bifidobacterium and lactobacillus. The preparing method is provided. The microecologics is in high strain activity, reliable effect, wide function, no toxic and side effect, irritation, etc.
Description
Technical field
The present invention relates to a kind of microbial ecological agent, more particularly relate to a kind of Tiny ecosystem high containing profitable probliotics and viable count
Preparation, belongs to biological medicament field.
Background technology
Microbial ecological agent is relative with " antibiotic " concept, has another name called active bacteria formulation or bacteria-promoting agent, refers to according to micro-
Ecological principle and the active bacteria formulation containing a large amount of probioticss made, the metabolite possibly together with them having or (with) add
Useful positive growth factor, has maintenance animal and plant and human body and the microecological balance of inside and outside environment thereof or adjusts its micro-life
State is lacked of proper care, and improves their health level and the function of protection environment.For relatively antibiotic, microbial ecological agent has nontoxic, without secondary
The feature such as act on, do not develop immunity to drugs, can effectively regulate microecological environment in human body, strengthen resistance of human body, treat multiple disease
Sick.The microorganism formulation that domestic market can be seen is tablet, powder or liquid oral liquid mostly, and manufacturing process is mostly passed through
Inactivation technology, therefore in medicine, viable bacteria content is low, and strain activity is poor, and therapeutic effect is undesirable.
Summary of the invention
The purpose of the present invention is aiming at above-mentioned defect of the prior art, it is provided that a kind of microbial ecological agent and preparation thereof
Method.
To achieve these goals, the technical scheme that the present invention provides is: a kind of microbial ecological agent, described microbial ecological agent
Being prepared from by following media and strain, culture medium configuration proportion is mineral water 100kg, soybean milk 40kg, steamed beef soup
30kg, glucose 3kg, vitamin C 0.2kg, strain is bacillus bifidus and lactobacillus.
The preparation method of described microbial ecological agent comprises the following steps:
1) bacillus bifidus and culture medium are seeded in the seed tank of sterilizing according to the volume ratio of 15%, cultivate 48 hours for 37 DEG C,
To bacillus bifidus seed liquid；
2) lactobacillus and culture medium are seeded in the seed tank of sterilizing according to the volume ratio of 10%, cultivate 24 hours for 37 DEG C,
To lactobacillus seed liquid；
3) bacillus bifidus seed liquid step 1) obtained and culture medium be inoculated into sterilizing according to the ratio of volume ratio 10% after send out
In ferment tank, obtain bacillus bifidus culture fluid；
4) by step 2) the lactobacillus seed liquid that obtains is inoculated into, according to the ratio of volume ratio 10%, the bifid bar that step 3) obtains
In bacteria culture fluid, cultivate 24 hours, i.e. obtain microbial ecological agent for 37 DEG C.
The invention have the benefit that and replace Chinese and western drugs with microbial ecological agent, do not hinder internal probiotic bacteria and cell, tissue
Organ, breaks away from the Chinese and western drugs toxic and side effects to human body；Strengthen population and the quantity of beneficial bacteria of intestinal tract, maintain the life that intestinal is good
State balances, and improves immunity, rehabilitation pathological changes；Microbial ecological agent processing technology is without inactivation technology, and in preparation, thalline quantity is many,
Activity is good.In a word, to provide technical scheme to have strain activity height, reliable effect, function extensive, without any side effects for the present invention
With series of advantages such as zests.
Detailed description of the invention
The present invention is further described in conjunction with the embodiments:
The present invention is a kind of microbial ecological agent, and concrete preparation method is as follows:
1) bacillus bifidus and culture medium are seeded in the seed tank of sterilizing according to the volume ratio of 15%, cultivate 48 hours for 37 DEG C,
To bacillus bifidus seed liquid；
2) lactobacillus and culture medium are seeded in the seed tank of sterilizing according to the volume ratio of 10%, cultivate 24 hours for 37 DEG C,
To lactobacillus seed liquid；
3) bacillus bifidus seed liquid step 1) obtained and culture medium be inoculated into sterilizing according to the ratio of volume ratio 10% after send out
In ferment tank, obtain bacillus bifidus culture fluid；
4) by step 2) the lactobacillus seed liquid that obtains is inoculated into, according to the ratio of volume ratio 10%, the bifid bar that step 3) obtains
In bacteria culture fluid, cultivate 24 hours, i.e. obtain microbial ecological agent for 37 DEG C.
Claims (2)
1. a microbial ecological agent, it is characterised in that: it is to be prepared from by following media and strain, culture medium configuration proportion
For mineral water 100kg, soybean milk 40kg, steamed beef soup 30kg, glucose 3kg, vitamin C 0.2kg, strain is bacillus bifidus
And lactobacillus.
The preparation method of a kind of microbial ecological agent the most according to claim 1, it is characterised in that comprise the following steps:
1) bacillus bifidus and culture medium are seeded in the seed tank of sterilizing according to the volume ratio of 15%, cultivate 48 hours for 37 DEG C,
To bacillus bifidus seed liquid；
2) lactobacillus and culture medium are seeded in the seed tank of sterilizing according to the volume ratio of 10%, cultivate 24 hours for 37 DEG C,
To lactobacillus seed liquid；
3) bacillus bifidus seed liquid step 1) obtained and culture medium be inoculated into sterilizing according to the ratio of volume ratio 10% after send out
In ferment tank, obtain bacillus bifidus culture fluid；
4) by step 2) the lactobacillus seed liquid that obtains is inoculated into, according to the ratio of volume ratio 10%, the bifid bar that step 3) obtains
In bacteria culture fluid, cultivate 24 hours, i.e. obtain microbial ecological agent for 37 DEG C.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610718899.XA CN106309505A (en) | 2016-08-25 | 2016-08-25 | Microecologics and preparing method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610718899.XA CN106309505A (en) | 2016-08-25 | 2016-08-25 | Microecologics and preparing method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106309505A true CN106309505A (en) | 2017-01-11 |
Family
ID=57791259
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610718899.XA Pending CN106309505A (en) | 2016-08-25 | 2016-08-25 | Microecologics and preparing method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106309505A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114631595A (en) * | 2022-04-18 | 2022-06-17 | 江苏恒丰强生物技术有限公司 | Veterinary composite microecological preparation and preparation method and application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101496822A (en) * | 2009-02-18 | 2009-08-05 | 上海谱莱生物技术有限公司 | Composite probiotics micro-ecological formulation and preparation method |
| CN101856369A (en) * | 2010-05-31 | 2010-10-13 | 遵义陆圣康源科技开发有限责任公司 | Stachyose tablets for calcium supplementation and intestinal micro-ecological regulation |
| CN103006713A (en) * | 2012-12-11 | 2013-04-03 | 内蒙古双奇药业股份有限公司 | Preparation method of microecological agent capable of reducing human body serum cholesterol content |
-
2016
- 2016-08-25 CN CN201610718899.XA patent/CN106309505A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101496822A (en) * | 2009-02-18 | 2009-08-05 | 上海谱莱生物技术有限公司 | Composite probiotics micro-ecological formulation and preparation method |
| CN101856369A (en) * | 2010-05-31 | 2010-10-13 | 遵义陆圣康源科技开发有限责任公司 | Stachyose tablets for calcium supplementation and intestinal micro-ecological regulation |
| CN103006713A (en) * | 2012-12-11 | 2013-04-03 | 内蒙古双奇药业股份有限公司 | Preparation method of microecological agent capable of reducing human body serum cholesterol content |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114631595A (en) * | 2022-04-18 | 2022-06-17 | 江苏恒丰强生物技术有限公司 | Veterinary composite microecological preparation and preparation method and application thereof |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170111 |