CN104222529A - Feed for preventing porcine reproductive and respiratory syndrome - Google Patents
Feed for preventing porcine reproductive and respiratory syndrome Download PDFInfo
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- CN104222529A CN104222529A CN201410536149.1A CN201410536149A CN104222529A CN 104222529 A CN104222529 A CN 104222529A CN 201410536149 A CN201410536149 A CN 201410536149A CN 104222529 A CN104222529 A CN 104222529A
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Abstract
The invention provides a feed for preventing porcine reproductive and respiratory syndrome. The feed includes the following raw materials: corn, bean pulp, salt, fish meal, soybean oil, zinc methionine, copper lysine, tryptophane, vitamin A, vitamin E, ethoxyquin and a traditional Chinese medicine feed additive for preventing the porcine reproductive and respiratory syndrome. The traditional Chinese medicine feed additive and the feed containing the traditional Chinese medicine feed additive have the advantages of being small in animal poisoning, less in drug residue and obvious in prevention and treatment effect compared with antiviral western medicine.
Description
Technical field
The present invention relates to field of feed, particularly relating to a kind of feed for preventing and treating porcine reproductive and respiratory syndrome.
Background technology
Porcine reproductive and respiratory syndrome (Porcine Reproductive and Respiration Syndrome, PRRS) be a kind of Acute exposure sexually transmitted disease caused by porcine reproductive and respiratory syndrome virus, mainly cause the breeding difficulty of in-pig and the respiratory symptom of piglet.Clinical symptoms main manifestations is sow heating, apocleisis, miscarriage or stillborn foetus, mummy tire, and Respiratory symptoms and high mortality often appear in piglet.Since this disease was found first from 1987, all over the world each is raised pigs state now, and the pathology that it causes causes catastrophic strike to pig industry, and this sick viral pathogenesis power is strong, and spread speed is fast.Swinery M & M is high, and the piglet incidence of disease can reach 100%, the death rate can reach more than 50%, and Sow abortion rate can reach more than 30%, and growing and fattening pigs, sow also can be fallen ill death.
The infection main damage macrophage of this disease, reduce immunity of organisms, cause scabies secondary infection, difficulty of prevention and cure is large. and use antibiotic and antiviral agent easily to produce pollution and toxic and side effect. mainly by vaccine inoculation, this disease is prevented at present, there is no effective medicine.Attenuated vaccine immunity effect is better, but it is dangerous itself to there is loose poison, and after inactivated vaccine inoculation, the titre that specific antibody produces is low, and speed is slow, can only provide part immunoprotection.
The protection antibody horizontal and the cellular immunity that improve vaccine immunity are the keys that anti-porcine reproductive and respiratory syndrome virus infects.Chinese medicine aboundresources, in treatment disease of viral infection, have unique advantage, more Chinese medicine has immunoregulation effect and antivirus action, as health care anti-disease medicines and additive, the immunologic function of body can be improved, improve premunition and the resistance of body.The single medicinal material of viral infection resisting has virus replication or deactivation, stops the effect of virus absorption onto cell.
Theory of traditional Chinese medical science is thought, though the generation of porcine reproductive and respiratory syndrome and change intricate, be attributed to 2 points, i.e. body internal factor and the interactional result of external factor.Traditional Chinese veterinary medicine thinks that this is the result that " healthy tendency " struggles against with " perverse trend "." healthy tendency " is the functional activity of each internal organs organ of livestock and poultry body, and adaptive capacity, the resistivity to paathogenic factor, the rehabilitation ability of its environment to external world, be the identification foreign matter based on the material such as function and qi-blood-body fluid, get rid of foreign matter, maintain self homeostasis and stable function; " perverse trend " refers to the extraneous six external factors which cause diseases, the gas of epidemic disease, and whether the health comprising again pathological change that body imbalance of yin and yang produces and pathological product body depends on the power of " healthy tendency " and " perverse trend ".From traditional Chinese veterinary medicine angle analysis, porcine reproductive and respiratory syndrome belongs to Epidemic Febrile Disease, and " empty evil mutual aid " is predisposing factors, and when the five internal organs that namely traditional Chinese medical science is said are in " void ", virus is taken advantage of a weak point, and causes pathogen being excessive but vital QI weak, disease-resistant unable; This disease is multiple for summer, how because of diseases caused by external factors damp and hotization fire, causes caused by Lung Qi obstruction, disturbance in ascending and descending, imbalance of yin and yang.In control, with strengthening vital QI to eliminate pathogenic factors, giving consideration to both the incidental and fundamental, by antiviral and immunity moderation function, realizes the preventing and controlling to this disease.Chinese medicine derives from natural, and poison the features such as little, medicament residue is few to animal, compare antiviral Western medicine, Chinese medicine has more obvious advantage in anti-reproductive and respiratory syndrome processed.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of Chinese medicine composition feed addictive for preventing and treating porcine reproductive and respiratory syndrome and the feed containing this Chinese medicine composition feed addictive, compare antiviral Western medicine, it is little to animal murder by poisoning, medicament residue is few, prevention and therapy successful, has advantage.
For solving the problems of the technologies described above, the present invention also provides a kind of feed for preventing and treating porcine reproductive and respiratory syndrome, and its raw material comprises corn, dregs of beans, salt, fish meal, soya-bean oil, zinc methionine, Copper lysinate, tryptophan, VitAVitE, ethoxyquinoline and the Chinese medicine composition feed addictive for preventing and treating porcine reproductive and respiratory syndrome.
Wherein, in described feed, the weight portion of each raw material is respectively corn 280 weight portion ~ 320 weight portion, dregs of beans 140 weight portion ~ 160 weight portion, salt 3 weight portion ~ 5 weight portion, fish meal 30 weight portion ~ 40 weight portion, soya-bean oil 20 weight portion ~ 30 weight portion, zinc methionine 0.5 weight portion ~ 1.5 weight portion, Copper lysinate 0.5 weight portion ~ 1.5 weight portion, tryptophan 0.5 weight portion ~ 1.5 weight portion, retinol1 weight portion ~ 2 weight portion, vitamin e1 weight portion ~ 2 weight portion, ethoxyquinoline 2 weight portion ~ 4 weight portion, described Chinese medicine composition feed addictive 50 weight portion ~ 70 weight portion.
Wherein, in described Chinese medicine composition feed addictive, each bulk drug comprises honeysuckle, cape jasmine, the wind-weed, Commeline communis, golden cypress, the red sage root, the vine of multiflower knotweed, the Radix Astragali, sweet wormwood, myrrh, giant knotweed, the membrane of a chicken's gizzard, eclipta, Radix Liriopes, Sculellaria barbata, Companumoea root, Samson St Johswort, parasitic loranthus, cassia twig, windproof, Centipeda minima, Chinese yam, Morinda officinalis, hairy euphorbia, Hygrophila salicifolia, Radix picrorrhizae and Radix Glycyrrhizae.
Wherein, in described Chinese medicine composition feed addictive, the weight of each bulk drug is respectively honeysuckle 55 weight portion ~ 65 weight portion, cape jasmine 30 weight portion ~ 40 weight portion, the wind-weed 25 weight portion ~ 35 weight portion, Commeline communis 20 weight portion ~ 30 weight portion, golden cypress 30 weight portion ~ 40 weight portion, the red sage root 25 weight portion ~ 35 weight portion, the vine of multiflower knotweed 30 weight portion ~ 40 weight portion, the Radix Astragali 55 weight portion ~ 65 weight portion, sweet wormwood 25 weight portion ~ 35 weight portion, myrrh 15 weight portion ~ 25 weight portion, giant knotweed 20 weight portion ~ 30 weight portion, the membrane of a chicken's gizzard 30 weight portion ~ 40 weight portion, eclipta 25 weight portion ~ 35 weight portion, Radix Liriopes 20 weight portion ~ 30 weight portion, Sculellaria barbata 25 weight portion ~ 35 weight portion, Companumoea root 20 weight portion ~ 30 weight portion, Samson St Johswort 25 weight portion ~ 35 weight portion, parasitic loranthus 30 weight portion ~ 40 weight portion, cassia twig 20 weight portion ~ 30 weight portion, windproof 25 weight portion ~ 35 weight portions, Centipeda minima 25 weight portion ~ 35 weight portion, Chinese yam 20 weight portion ~ 30 weight portion, Morinda officinalis 20 weight portion ~ 30 weight portion, hairy euphorbia 25 weight portion ~ 35 weight portion, Hygrophila salicifolia 20 weight portion ~ 30 weight portion, Radix picrorrhizae 15 weight portion ~ 25 weight portion and Radix Glycyrrhizae 20 weight portion ~ 30 weight portion.
Wherein, in described Chinese medicine composition feed addictive, the weight of each bulk drug is respectively honeysuckle 60 weight portion, cape jasmine 35 weight portion, the wind-weed 30 weight portion, Commeline communis 25 weight portion, golden cypress 35 weight portion, the red sage root 30 weight portion, the vine of multiflower knotweed 35 weight portion, the Radix Astragali 60 weight portion, sweet wormwood 30 weight portion, myrrh 20 weight portion, giant knotweed 25 weight portion, the membrane of a chicken's gizzard 35 weight portion, eclipta 30 weight portion, Radix Liriopes 25 weight portion, Sculellaria barbata 30 weight portion, Companumoea root 25 weight portion, Samson St Johswort 30 weight portion, parasitic loranthus 35 weight portion, cassia twig 25 weight portion, windproof 30 weight portions, Centipeda minima 30 weight portion, Chinese yam 25 weight portion, Morinda officinalis 25 weight portion, hairy euphorbia 30 weight portion, Hygrophila salicifolia 25 weight portion, Radix picrorrhizae 20 weight portion and Radix Glycyrrhizae 25 weight portion.
Present invention also offers the preparation method of above-mentioned feed, be specially:
The first step, mixes by weight by each raw material components, gets 100g mixture, add the water of 500g ~ 600g, boil 3 ~ 4 hours, filter, obtain the first filtrate, filter residue adds the water of 200g ~ 300g again, again boils 1 ~ 2 hour, filter, obtain the second filtrate, by the first filtrate and the mixing of the second filtrate, concentrated removing aqueous solvent, pulverize, dry, obtain Chinese medicine composition fodder additive;
Second step, puts into fodder mixing machine together with the described Chinese medicine composition fodder additive other raw material components in feedstuff and the first step obtained, and continuous uniform stirred after 1 ~ 3 hour, put into pulverizer and pulverized, and obtains feed.
Present invention also offers a kind of Chinese medicine composition for preventing and treating porcine reproductive and respiratory syndrome, its bulk drug comprises honeysuckle, cape jasmine, the wind-weed, Commeline communis, golden cypress, the red sage root, the vine of multiflower knotweed, the Radix Astragali, sweet wormwood, myrrh, giant knotweed, the membrane of a chicken's gizzard, eclipta, Radix Liriopes, Sculellaria barbata, Companumoea root, Samson St Johswort, parasitic loranthus, cassia twig, windproof, Centipeda minima, Chinese yam, Morinda officinalis, hairy euphorbia, Hygrophila salicifolia, Radix picrorrhizae and Radix Glycyrrhizae.
Wherein, in described Chinese medicine composition, the weight of each bulk drug is respectively honeysuckle 55 weight portion ~ 65 weight portion, cape jasmine 30 weight portion ~ 40 weight portion, the wind-weed 25 weight portion ~ 35 weight portion, Commeline communis 20 weight portion ~ 30 weight portion, golden cypress 30 weight portion ~ 40 weight portion, the red sage root 25 weight portion ~ 35 weight portion, the vine of multiflower knotweed 30 weight portion ~ 40 weight portion, the Radix Astragali 55 weight portion ~ 65 weight portion, sweet wormwood 25 weight portion ~ 35 weight portion, myrrh 15 weight portion ~ 25 weight portion, giant knotweed 20 weight portion ~ 30 weight portion, the membrane of a chicken's gizzard 30 weight portion ~ 40 weight portion, eclipta 25 weight portion ~ 35 weight portion, Radix Liriopes 20 weight portion ~ 30 weight portion, Sculellaria barbata 25 weight portion ~ 35 weight portion, Companumoea root 20 weight portion ~ 30 weight portion, Samson St Johswort 25 weight portion ~ 35 weight portion, parasitic loranthus 30 weight portion ~ 40 weight portion, cassia twig 20 weight portion ~ 30 weight portion, windproof 25 weight portion ~ 35 weight portions, Centipeda minima 25 weight portion ~ 35 weight portion, Chinese yam 20 weight portion ~ 30 weight portion, Morinda officinalis 20 weight portion ~ 30 weight portion, hairy euphorbia 25 weight portion ~ 35 weight portion, Hygrophila salicifolia 20 weight portion ~ 30 weight portion, Radix picrorrhizae 15 weight portion ~ 25 weight portion and Radix Glycyrrhizae 20 weight portion ~ 30 weight portion.
Present invention also offers the preparation method of above-mentioned Chinese medicine composition, be specially: each raw material components is mixed by weight, get 100g mixture, add the water of 500g ~ 600g, boil 3 ~ 4 hours, filter, obtain the first filtrate, filter residue adds the water of 200g ~ 300g again, again boils 1 ~ 2 hour, filter, obtain the second filtrate, by the first filtrate and the mixing of the second filtrate, concentrated removing aqueous solvent, pulverize, dry, obtain Chinese medicine composition.
Beneficial effect of the present invention:
Chinese medicine composition feed addictive for preventing and treating porcine reproductive and respiratory syndrome provided by the invention and the feed containing this Chinese medicine composition feed addictive, compare antiviral Western medicine, it is little to animal murder by poisoning, medicament residue is few, and prevention and therapy successful, has advantage.
Detailed description of the invention
The invention provides a kind of Chinese medicine composition for preventing and treating porcine reproductive and respiratory syndrome, its bulk drug comprises honeysuckle, cape jasmine, the wind-weed, Commeline communis, golden cypress, the red sage root, the vine of multiflower knotweed, the Radix Astragali, sweet wormwood, myrrh, giant knotweed, the membrane of a chicken's gizzard, eclipta, Radix Liriopes, Sculellaria barbata, Companumoea root, Samson St Johswort, parasitic loranthus, cassia twig, windproof, Centipeda minima, Chinese yam, Morinda officinalis, hairy euphorbia, Hygrophila salicifolia, Radix picrorrhizae and Radix Glycyrrhizae.
Further preferably, described Chinese medicine composition feed addictive is only made up of above-mentioned raw materials medicine.
In described Chinese medicine composition, the weight of each bulk drug is respectively honeysuckle 55 weight portion ~ 65 weight portion, cape jasmine 30 weight portion ~ 40 weight portion, the wind-weed 25 weight portion ~ 35 weight portion, Commeline communis 20 weight portion ~ 30 weight portion, golden cypress 30 weight portion ~ 40 weight portion, the red sage root 25 weight portion ~ 35 weight portion, the vine of multiflower knotweed 30 weight portion ~ 40 weight portion, the Radix Astragali 55 weight portion ~ 65 weight portion, sweet wormwood 25 weight portion ~ 35 weight portion, myrrh 15 weight portion ~ 25 weight portion, giant knotweed 20 weight portion ~ 30 weight portion, the membrane of a chicken's gizzard 30 weight portion ~ 40 weight portion, eclipta 25 weight portion ~ 35 weight portion, Radix Liriopes 20 weight portion ~ 30 weight portion, Sculellaria barbata 25 weight portion ~ 35 weight portion, Companumoea root 20 weight portion ~ 30 weight portion, Samson St Johswort 25 weight portion ~ 35 weight portion, parasitic loranthus 30 weight portion ~ 40 weight portion, cassia twig 20 weight portion ~ 30 weight portion, windproof 25 weight portion ~ 35 weight portions, Centipeda minima 25 weight portion ~ 35 weight portion, Chinese yam 20 weight portion ~ 30 weight portion, Morinda officinalis 20 weight portion ~ 30 weight portion, hairy euphorbia 25 weight portion ~ 35 weight portion, Hygrophila salicifolia 20 weight portion ~ 30 weight portion, Radix picrorrhizae 15 weight portion ~ 25 weight portion and Radix Glycyrrhizae 20 weight portion ~ 30 weight portion.
Further, in described Chinese medicine composition, the weight of each bulk drug is respectively honeysuckle 58 weight portion ~ 62 weight portion, cape jasmine 33 weight portion ~ 37 weight portion, the wind-weed 28 weight portion ~ 32 weight portion, Commeline communis 23 weight portion ~ 27 weight portion, golden cypress 33 weight portion ~ 37 weight portion, the red sage root 28 weight portion ~ 32 weight portion, the vine of multiflower knotweed 33 weight portion ~ 37 weight portion, the Radix Astragali 58 weight portion ~ 62 weight portion, sweet wormwood 28 weight portion ~ 32 weight portion, myrrh 18 weight portion ~ 22 weight portion, giant knotweed 23 weight portion ~ 27 weight portion, the membrane of a chicken's gizzard 33 weight portion ~ 37 weight portion, eclipta 28 weight portion ~ 32 weight portion, Radix Liriopes 23 weight portion ~ 27 weight portion, Sculellaria barbata 28 weight portion ~ 32 weight portion, Companumoea root 23 weight portion ~ 27 weight portion, Samson St Johswort 28 weight portion ~ 32 weight portion, parasitic loranthus 33 weight portion ~ 37 weight portion, cassia twig 23 weight portion ~ 27 weight portion, windproof 28 weight portion ~ 32 weight portions, Centipeda minima 28 weight portion ~ 32 weight portion, Chinese yam 23 weight portion ~ 27 weight portion, Morinda officinalis 23 weight portion ~ 27 weight portion, hairy euphorbia 28 weight portion ~ 32 weight portion, Hygrophila salicifolia 23 weight portion ~ 27 weight portion, Radix picrorrhizae 18 weight portion ~ 22 weight portion and Radix Glycyrrhizae 23 weight portion ~ 27 weight portion.
Most preferably, in described Chinese medicine composition, the weight of each bulk drug is respectively honeysuckle 60 weight portion, cape jasmine 35 weight portion, the wind-weed 30 weight portion, Commeline communis 25 weight portion, golden cypress 35 weight portion, the red sage root 30 weight portion, the vine of multiflower knotweed 35 weight portion, the Radix Astragali 60 weight portion, sweet wormwood 30 weight portion, myrrh 20 weight portion, giant knotweed 25 weight portion, the membrane of a chicken's gizzard 35 weight portion, eclipta 30 weight portion, Radix Liriopes 25 weight portion, Sculellaria barbata 30 weight portion, Companumoea root 25 weight portion, Samson St Johswort 30 weight portion, parasitic loranthus 35 weight portion, cassia twig 25 weight portion, windproof 30 weight portions, Centipeda minima 30 weight portion, Chinese yam 25 weight portion, Morinda officinalis 25 weight portion, hairy euphorbia 30 weight portion, Hygrophila salicifolia 25 weight portion, Radix picrorrhizae 20 weight portion and Radix Glycyrrhizae 25 weight portion.
Present invention also offers the preparation method of above-mentioned Chinese medicine composition, be specially:
Each raw material components is mixed by weight, gets 100g mixture, add the water of 500g ~ 600g, boil 3 ~ 4 hours, filter, obtain the first filtrate, filter residue adds the water of 200g ~ 300g again, again boils 1 ~ 2 hour, filters, obtain the second filtrate, by the first filtrate and the mixing of the second filtrate, concentrated removing aqueous solvent, pulverizes, drying, obtains Powdered additive.
This Chinese medicine composition uses as feed addictive.
Present invention also offers the parenteral solution formulation of above-mentioned Chinese medicine composition, its bulk drug comprises honeysuckle, cape jasmine, the wind-weed, Commeline communis, golden cypress, the red sage root, the vine of multiflower knotweed, the Radix Astragali, sweet wormwood, myrrh, giant knotweed, the membrane of a chicken's gizzard, eclipta, Radix Liriopes, Sculellaria barbata, Companumoea root, Samson St Johswort, parasitic loranthus, cassia twig, windproof, Centipeda minima, Chinese yam, Morinda officinalis, hairy euphorbia, Hygrophila salicifolia, Radix picrorrhizae and Radix Glycyrrhizae, its preparation method is specially: mixed by weight by each raw material components, get 100g mixture, add the water of 500g ~ 600g, boil 3 ~ 4 hours, filter, obtain the first filtrate, filter residue adds the water of 200g ~ 300g again, again boil 1 ~ 2 hour, filter, obtain the second filtrate, by the first filtrate and the mixing of the second filtrate, concentrated removing aqueous solvent, pulverize, dry, obtain Chinese medicine composition, described Chinese medicine composition is dissolved in water, adjust pH 7.0, filter, embedding, sterilizing, obtaining crude drug concentration is the parenteral solution of 1g/ml.
The present invention also provides a kind of feed for preventing and treating porcine reproductive and respiratory syndrome, and its raw material comprises corn, dregs of beans, salt, fish meal, soya-bean oil, zinc methionine, Copper lysinate, tryptophan, VitAVitE, ethoxyquinoline and the Chinese medicine composition feed addictive for preventing and treating porcine reproductive and respiratory syndrome.
Further, in described feed, the weight portion of each raw material is respectively corn 280 weight portion ~ 320 weight portion, dregs of beans 140 weight portion ~ 160 weight portion, salt 3 weight portion ~ 5 weight portion, fish meal 30 weight portion ~ 40 weight portion, soya-bean oil 20 weight portion ~ 30 weight portion, zinc methionine 0.5 weight portion ~ 1.5 weight portion, Copper lysinate 0.5 weight portion ~ 1.5 weight portion, tryptophan 0.5 weight portion ~ 1.5 weight portion, retinol1 weight portion ~ 2 weight portion, vitamin e1 weight portion ~ 2 weight portion, ethoxyquinoline 2 weight portion ~ 4 weight portion, Chinese medicine composition feed addictive 50 weight portion ~ 70 weight portion.
Further, in described feed, the weight portion of each raw material is respectively corn 300 weight portion, dregs of beans 150 weight portion, salt 4 weight portion, fish meal 35 weight portion, soya-bean oil 25 weight portion, zinc methionine 1 weight portion, Copper lysinate 1 weight portion, tryptophan 1 weight portion, vitamin A 2 weight portion, vitamin E 2 weight portion, ethoxyquinoline 3 weight portion, Chinese medicine composition feed addictive 60 weight portion.
Present invention also offers the preparation method of above-mentioned feed, be specially:
The first step, prepares described Chinese medicine composition feed addictive;
Second step, puts into fodder mixing machine together with the described Chinese medicine composition feed addictive other raw material components in feedstuff and the first step obtained, and continuous uniform stirred after 1 ~ 3 hour, put into pulverizer and pulverized, and obtains feed.
The pharmacology of described each Chinese medicine material medicine is as follows:
Honeysuckle: latin name lonicerae japonicae flos, taste is sweet, cold in nature, enters the heart, lung, stomach, large intestine channel, has clearing heat and detoxicating effect, doublely can remove heat from the blood and relieve diarrhea.Be mainly used in affection of exogenous wind-heat, the hot poison of warm heat disease is comparatively contained, or when warm disease is still not descending at the part of the body cavity above the diaphragm housing the heart and lungs from the beginning of heat evil; The malicious diarrhea of heat, the lower blood of stool; Heat temperature is demonstrate,proved; The sore carbuncle that hot poison gathers is from the beginning of the, inflammation heat person of swelling and ache etc., there is good antipyretic effect, energy anti-inflammatory, what control inflammation oozes out and inflammatory hyperplasia, leukocytic phagocytic function can be promoted, there is stronger bacteriostasis to multiple pyococcus and pathogenic entero becteria, comprise staphylococcus, hemolytic streptococcus, diplococcus meningitidis, shigella dysenteriae, typhoid bacillus, Pseudomonas aeruginosa, tubercle bacillus etc.
Cape jasmine: latin name gardeniae fructus, bitter, cold in nature, enter the heart, lung, three burnt, have the effect of relieving restlessness purging intense heat, soothing liver-qi stagnation, clearing heat and promoting diuresis, cooling blood and hemostasis, be mainly used in the vexed uneasiness of the strongly fragrant chest gastral cavity of warm heat disease heat, bother not peaceful; Liver depression is not relaxed or Liver Channel has having a dizzy spell of heat, pain in chest and hypochondrium, hot eyes dry throat; Jaundice with damp-heat pathogen; The cards such as the abdominal distention of qi depression to blood stasis.
The wind-weed: bitter, sweet, cold in nature, enters lung, stomach, kidney channel, has relieving restlessness of purging heat, the effect of nourshing Yin and drynsessmoistening prescription, removing heat from the lung to relieve cough, and cure mainly fever caused by exogenous pathogenic factors, high heat is irritated, lung-heat type cough, and interior heat such as to be quenched one's thirst at the symptom; Its bitter cold matter is moistened, and property sedimentation can heat-clearing, can promote the production of body fluid again, kindly controls warm disease and evil and enters gas and divide, and heat is contained and hindered Tianjin; Also diabetes is used for the treatment of; Phlegm-heat obstructing lung and breathing heavily, xeropulmonary cough, phlegm is few unfavorable; Fire excess from yin deficiency, the cards such as flaring up of deficient fire.
Commeline communis: taste is sweet, light, cold in nature, enter the heart, liver,spleen,kidney, intestine and small intestine warp, have clearing heat and detoxicating, effect of inducing diuresis for removing edema.Cure mainly oedema, beriberi, difficult urination, flu, erysipelas, mumps, icterohepatitis, hot dysentery, malaria, nosebleed epistaxis, hematuria, metrorrhagia, leukorrhea, abscess of throat, the cards such as ulcer furunculosis.
Golden cypress: bitter, cold in nature, enter kidney, bladder, large intestine channel, heat-clearing and damp-drying drug, purging intense heat and detonicating, except hectic fever due to yin clearind deficient heat.For damp-heat dysentery, jaundice, under band, heat is drenched, beriberi, and impotence is warded off, hectic fever due to yin labor heat, night sweat, seminal emission, sore swollen toxin, eczema itch.Stir-baked CORTEX PHELLODENDRI with salt solution is nourishing Yin and falling fire.For fire excess from yin deficiency, night sweat hectic fever due to yin.
The red sage root: bitter, cold nature, enters the heart, Liver Channel, has promoting blood circulation for regulating menstruation, stasis-dispelling and pain-killing, cool blood disappears carbuncle, and clear away heart-fire relieving restlessness, the effect of nourishing blood and tranquilization, for irregular menstruation, dysmenorrhoea, through closing, the stagnant stomachache of the stasis of blood in postpartum, trusted subordinate's pain, hot numbness swells and ache, traumatic injury, and pyreticosis is irritated, the cards such as palpitation and insomnia, red sage root energy coronary artery dilator, increases coronary flow, improves myocardial ischemia, infraction and cardiac function, regulate the rhythm of the heart, and can peripheral vascular be expanded, improve microcirculation; Body's hypoxia tolerance can be improved; There is anticoagulation, promote fibrinolytic, suppress platelet aggregation, the effect of inhibition thrombosis; Can blood fat be reduced, suppress coronary atherosclerosis to be formed; Can suppress or alleviate degeneration of liver cells, necrosis and inflammatory reaction, promote liver cell regeneration, and have anti-fibrosis effect; The convalescence of red blood cell and hemochrome can be shortened, Reticulocyte is increased, the reparation organized can be promoted, accelerate the healing of fracture; Nervous centralis is had to have inhibitory action; There is antitumor action; Energy enhanced machine body immunity function; Blood sugar can be reduced; Inhibitory action is had to various bacteria such as tubercle bacillus, Gold production staphylococcuses.
The vine of multiflower knotweed: sweet, the micro-hardship of taste, property is put down; The thoughts of returning home, Liver Channel, have effect of energy mental-tranquilization, dispelling wind and removing obstruction in the meridians, cure mainly insomnia and dreamful sleep, deficiency of blood bodily pain, numbness, arthralgia due to wind-dampness, the cards such as wind sore mange.
The Radix Astragali: latin name astragali radix, taste is sweet, warm in nature, returns lung, the spleen channel, has help spleen soil, elevate a turnable ladder yang-energy, strengthening exterior and reducing sweat, the effects such as holder sore myogenic, are mainly used in treating spleen-lung Qi deficiency caused weak anorexia and loose stool, palpitation, the visceral organ ptosis of the sinking of qi of middle-jiao, Qi dysfunction in blood control uterine bleeding is had blood in stool, and rushes down prolapse of the anus for a long time; The spontaneous sweating of exterior deficiency; Insufficiency of vital energy and blood, yang-energy sore on the decline does not rise, purulence becomes not burst, burst and do not hold back for a long time; And the deficiency of vital energy loses the oedema of fortune, the cards such as difficult urination.
Sweet wormwood: latin name Artemisiae Annuae Herba, bitter, pungent, cold in nature, return liver, gallbladder channel, have abnormal heat clearly thoroughly, cool blood is except steaming, and except steaming, effect of preventing malaria, cures mainly summer-heat evil heating, fever due to yin deficiency, night fever abating at dawn, hectic fever due to yin labor heat, malaria fever and chills, the cards such as jaundice with damp-heat pathogen.
Myrrh: bitter, pungent, property is put down, and enters liver, spleen, the heart, kidney channel, has effect of promoting blood circulation and stopping pain, detumescence and promoting granulation, cures mainly chest abdomen stasis of blood pain, dysmenorrhoea, through closing, abdominal mass, traumatic injury, carbuncle swell the cards such as sore, acute appendicitis, red eye, swell pain.
Giant knotweed: latin name Polygoni Cuspidati Rhizoma et Radix, micro-hardship, is slightly cold.Return liver, courage, lung channel, have clearing heat and detoxicating, normalizing gallbladder to cure jaundice, expelling wind and removing dampness, loose stasis of blood analgesic therapy, relieving cough and reducing sputum effect, for joint pain, jaundice with damp-heat pathogen, through Bi ， wei lump in the abdomen, coughing with a lot of sputum, scald, injury from falling down, the cards such as carbuncle sore tumefacting virus.
The membrane of a chicken's gizzard: taste is sweet, cold in nature, return spleen, stomach, small intestine, urinary bladder channel, have reinforcing spleen to promote digestion, puckery essence stops the effect of drowning, cure mainly dyspepsia turgor, vomiting gastric disorder causing nausea, rushes down dysentery, infantile malnutrition due to digestive disturbances or intestinalparasites, quenches one's thirst, and loses and drowns, larynx numbness acute tonsillitis, the cards such as noma aphtha.
Eclipta: taste is sweet, sour, cold in nature, returns liver, kidney channel, plays not liver and kidney benefiting, the effect of cooling blood and hemostasis, for having a dizzy spell caused by the deficiency of liver-yin and kidney-yin, blurring of vision, and soreness and weakness of waist and knees; Be suitable for the deficiency of Yin and have hot person, be used for the treatment of the haematemesis of accumulated heat in stomach, the cards such as the hemoptysis of heat impairing pulmonary collateral.
Radix Liriopes: latin name liriopes radix, sweet, the micro-hardship of taste, cold nature, the thoughts of returning home, lung, stomach warp, have nourishing Yin and promoting production of body fluid, effect that moistening lung clears away heart-fire, and for dryness of the lung dry cough, cough due to consumptive disease, Tianjin wound is thirsty, vexed insomnia, the cards such as the dry constipation of intestines.
Sculellaria barbata: taste is pungent, bitter, cold in nature, enters lung channel, Liver Channel, kidney channel, has the effect of clearing heat and detoxicating, loose stasis of blood hemostasis, inducing diuresis to reduce edema, cures mainly: hot malicious carbuncle swells; Have sore throat; Lung carbuncle; Acute appendicitis; Scrofula; Venomous snake bite; Traumatic injury; Spit blood; Bleeding from five sense organs or subcutaneous tissue; Blood drenches; Oedema; Ascites and cancer.
Companumoea root: latin name radix campanumoea javanicae, be the root of Campanulaceae leopard platymiscium Campanumoea javanica and leopard, taste is sweet, property is put down, and returns lung, the spleen channel, has strengthening the spleen and replenishing qi, tonifies the lung to relieve cough, effect of lactogenesis, cure mainly the empty internal injury of labor, the deficiency of vital energy is weak, palpitaition, hidrosis, splenasthenic diarrhea, leukorrhea, milk is rare, the cards such as insufficiency of the spleen cough.
Samson St Johswort: the herb for Guttiferae hypericum Samson St Johswort (nontoxic): bitter, pungent, cold in nature, return liver, the spleen channel, there is removing pattogenic heat from the blood and toxic material from the body, promoting blood circulation for regulating menstruation, effect of dispelling wind and removing obstruction in the meridians, cure mainly various hemorrhage, enteritis and dysentery, acute mastitis, carbuncle swells serious case of furuncle, scalds, snake bite, irregular menstruation, dysmenorrhoea, leukorrhea, traumatic injury, arthralgia due to wind-dampness, the cards such as aphtha.
Parasitic loranthus: bitter, sweet, property is put down, and enters liver, kidney channel, have the effect of filling liver kidney, strengthening the bones and muscles, wines used as antirheumatic, antiabortive unit, for the soreness of waist pain caused by kidney deficiency and liver, muscles and bones impotence is soft, the foot and knee being feeble, kidney deficiency and liver, essence and blood void is lost, the fetal irritability of liability to be abortion, the cards such as waist abdomen pain.
Cassia twig: be the dry spray of canella Chinese cassia tree: latin name Cinnamomi Ramulus, taste is pungent, sweet, warm in nature, the thoughts of returning home, lung, urinary bladder channel, has sweating expelling pathogenic factors from muscles and skin, warming meridians and promoting circulation of qi, helps yang transforming qi, effect of eliminating cold to stop pain.
Windproof: taste is pungent, sweet, slightly warm in nature, return bladder, liver, the spleen channel, have and expel pathogenic wind from the body surface, removing dampness to relieve pain, stop effect that convulsion is jerked surely, cure mainly diseases caused by external factors illness that has not attacked the vital organs of the human body, rubella itch, arthralgia due to wind-dampness, the cards such as lockjaw.
Centipeda minima: taste is pungent, warm in nature, enter lung, Liver Channel, there is cold-dispelling, clearing the nasal passage, cough-relieving apophlegmatic, the effect of eliminating stasis to subdue swelling, for exterior syndrome due to wind-cold, nasal obstruction runny nose and nasosinusitis, coughing with a lot of sputum, pertussis, the cards such as sore, carbuncle and painful swelling.
Chinese yam: latin name dioscoreae rhizoma, taste is sweet, property is flat, and enter lung, spleen, stomach warp, have tonifying spleen nourishing the stomach, beneficial lung reinforces the kidney, and the effect of nourishing Yin and promoting production of body fluid cures mainly weakness of the spleen and the stomach, and the cards such as difference, the vexed diarrhoea of gastral cavity received less by the food of the dysfunction of the spleen in transport.
Morinda officinalis: taste is pungent, sweet, slightly warm in nature, enters liver, kidney channel, has the effect of kidney-replenishing, strengthening the bones and muscles, wines used as antirheumatic, and for impotence due to deficiency of the kidney, seminal emission premature ejaculation, few abdomen crymodynia, palace is cold infertile, the cards such as the muscles and bones impotence caused by kidney deficiency and liver is soft, waist knee crymodynia.
Hairy euphorbia: mildly bitter flavor, micro-acid, cool in nature, have clearing heat and detoxicating, effect that dampness removing is antipruritic, for bacillary dysentery, amoebic dysentery, enteritis, enteron aisle trichomonad, indigestion, bronchitis, pyelonephritis; The cards such as eczema, dermatitis, pruitus are controlled in external application.
Hygrophila salicifolia: sweet, the micro-hardship of taste, cool in nature, have clearing heat and detoxicating, effect of eliminating stasis to subdue swelling, row heat poison when curing mainly, erysipelas, jaundice, aphtha, abscess of throat, acute mastitis, traumatic injury, fracture, snake venom such as to bite at the card.
Radix picrorrhizae: latin name Picrorhizae Rhizoma, bitter, cold in nature, return liver, stomach, large intestine channel, have reducing the asthenic fever, treating infantile malnutrition wide fever, effect of clearing away damp-heat, cures mainly osteopyrexia and fever, children's's infantile malnutrition due to digestive disturbances or intestinalparasites heat, and damp-heat dysentery, jaundice urine is red, the cards such as hemorrhoid gall.
Radix Glycyrrhizae: latin name Glycyrrhizae Radix et Rhizoma, taste is sweet, property put down, enter 12 warps, have clearing heat and detoxicating, be in harmonious proportion the property of medicine, the effect of expelling phlegm and arresting coughing, and can invigorating the spleen and in, relieving spasm to stop pain, is mainly used in weakness of the spleen and the stomach abdominal distension and anorexia, weak; Contraction pain in abdomen being alleviated, relax the strong and toxicity of some drugs, alleviate medicine to the toxic and side effect of body or to GI stimulation, tonifying Qi neutralization can be played by adding Radix Glycyrrhizae, be in harmonious proportion the work of each component.
Below adopt embodiment to describe embodiments of the present invention in detail, to the present invention, how application technology means solve technical problem whereby, and the implementation procedure reaching technique effect can fully understand and implement according to this.
Embodiment 1 Chinese medicine composition feed addictive 1
By honeysuckle 60g, cape jasmine 35g, wind-weed 30g, Commeline communis 25g, golden cypress 35g, red sage root 30g, vine of multiflower knotweed 35g, Radix Astragali 60g, sweet wormwood 30g, myrrh 20g, giant knotweed 25g, the membrane of a chicken's gizzard 35g, eclipta 30g, Radix Liriopes 25g, Sculellaria barbata 30g, Companumoea root 25g, Samson St Johswort 30g, parasitic loranthus 35g, cassia twig 25g, windproof 30g, Centipeda minima 30g, Chinese yam 25g, Morinda officinalis 25g, hairy euphorbia 30g, Hygrophila salicifolia 25g, Radix picrorrhizae 20g and Radix Glycyrrhizae 25g mixes, get 100g mixture, add the water of 600g, boil 4 hours, filter, obtain the first filtrate, filter residue adds the water of 300g again, again boil 2 hours, filter, obtain the second filtrate, by the first filtrate and the mixing of the second filtrate, concentrated removing aqueous solvent, pulverize, dry, obtain Powdered additive.
Embodiment 2 Chinese medicine composition feed addictive 2
By honeysuckle 55g, cape jasmine 30g, wind-weed 25g, Commeline communis 20g, golden cypress 30g, red sage root 25g, vine of multiflower knotweed 30g, Radix Astragali 55g, sweet wormwood 25g, myrrh 15g, giant knotweed 20g, the membrane of a chicken's gizzard 30g, eclipta 25g, Radix Liriopes 20g, Sculellaria barbata 25g, Companumoea root 20g, Samson St Johswort 25g, parasitic loranthus 30g, cassia twig 20g, windproof 25g, Centipeda minima 25g, Chinese yam 20g, Morinda officinalis 20g, hairy euphorbia 25g, Hygrophila salicifolia 20g, Radix picrorrhizae 15g and Radix Glycyrrhizae 20g mixes, get 100g mixture, add the water of 600g, boil 4 hours, filter, obtain the first filtrate, filter residue adds the water of 300g again, again boil 2 hours, filter, obtain the second filtrate, by the first filtrate and the mixing of the second filtrate, concentrated removing aqueous solvent, pulverize, dry, obtain Powdered additive.
Embodiment 3 Chinese medicine composition feed addictive 3
By honeysuckle 65g, cape jasmine 40g, wind-weed 35g, Commeline communis 30g, golden cypress 40g, red sage root 35g, vine of multiflower knotweed 40g, Radix Astragali 65g, sweet wormwood 35g, myrrh 25g, giant knotweed 30g, the membrane of a chicken's gizzard 40g, eclipta 35g, Radix Liriopes 30g, Sculellaria barbata 35g, Companumoea root 30g, Samson St Johswort 35g, parasitic loranthus 40g, cassia twig 30g, windproof 35g, Centipeda minima 35g, Chinese yam 30g, Morinda officinalis 30g, hairy euphorbia 35g, Hygrophila salicifolia 30g, Radix picrorrhizae 25g and Radix Glycyrrhizae 30g mixes, get 100g mixture, add the water of 600g, boil 4 hours, filter, obtain the first filtrate, filter residue adds the water of 300g again, again boil 2 hours, filter, obtain the second filtrate, by the first filtrate and the mixing of the second filtrate, concentrated removing aqueous solvent, pulverize, dry, obtain Powdered additive.
Embodiment 4 pharmacological toxicity is tested
Acute toxicity test data
Detect reagent
Serum AST, ALT detection kit are purchased from Rui Sheng bio tech ltd, Qingdao.
Checkout equipment
Micro-200 semiautomatic biochemistry detector, assay balance, disscting instrument, syringe, test tube etc.
Test method
After the adaptability of 3 days are raised, be divided at random 3 groups (male and female half and half), often organize each 20; Control group, 2 different pharmaceutical groups (are respectively 400g/kg body weight, 300g/kg body weight), control group gavage physiological saline, the Chinese medicine composition feed addictive 1 of 2 the medicine group gavage embodiment of the present invention 1 preparations, is dissolved in Chinese medicine composition feed addictive in water and gavages mouse.
Testing index and method
(1) clinicing symptom observation: every day viewing test mouse the state of mind, diet situation, every day entry morbidity death condition.
(2) pathological observation: dead mouse is carried out cuing open inspection and observes internal organ change.
(3) growth indexes is observed: carry out routine weighing to test mouse; Off-test, slaughters that mouse weighs in, the heavy organ index of internal organs measures: cut open inspection rat, core dirty, spleen, liver, kidney, testis, ovary, weigh, calculate organ index according to the following formula: organ index=internal organs weight/body weight × 100.
(4) liver function change is observed: after off-test, slaughter mouse, take blood separation of serum, measures AST, ALT.
Data process&analysis
Each group of data all use mean soil standard deviation
represent, each group mean does test of normality and duplicate measurements homogeneity test of variance, with inspection younger between one-way analysis of variance group), P < 0.05 is for differing significantly, and P < 0.01 is difference highly significant.Statistical computation all adopts SPSS12.0 software to complete.
Clinicing symptom observation
Control group and test group mouse, the state of mind and diet wish, to external world respond have no obvious exception.Each group of mouse morbidity and death condition are as following table 1.
Table 1
| Grouping | Sum | Dead (only) | The death rate (%) |
| 1(400g/kg) | 20 | 0 | 0 |
| 2(300g/kg) | 20 | 0 | 0 |
| Control group | 20 | 0 | 0 |
Pathological observation: control group and test group, mouse carries out cuing open inspection and observes to have no and observe the obvious pathological change of internal organs after slaughtering.
Growth indexes is observed: off-test, slaughters that mouse weighs in, internal organs weight analysis is in table 2.
Table 2
| Grouping | Liver index | Cardiac index | Renal index | Lungs index | Index and spleen index |
| 1(400g/kg) | 5.79±0.22 | 0.83±0.08 | 1.23±0.21 | 0.72±0.09 | 0.51±0.06 |
| 2(300g/kg) | 5.73±0.17 | 0.80±0.11 | 1.21±0.19 | 0.74±0.12 | 0.52±0.08 |
| Control group | 5.72±0.25 | 0.82±0.09 | 1.26±0.25 | 0.73±0.10 | 0.50±0.05 |
Liver function change is observed
After off-test, slaughter mouse, take blood separation of serum, measure AST (glutamic-oxalacetic transaminease), ALT (glutamic-pyruvic transaminase) in table 3.It is not remarkable that result shows each group of data difference compared with normal healthy controls group, is all in normal range (NR).
Table 3
| Grouping | Sum | ALT | AST |
| 1(400g/kg) | 20 | 30.91±2.17 | 132.11±10.32 |
| 2(300g/kg) | 20 | 32.63±2.53 | 130.57±9.96 |
| Control group | 20 | 31.97±2.09 | 131.91±12.03 |
Long term toxicity test data
Chinese medicine composition feed addictive 20g/kg, 100g/kg of the present invention of preparing according to embodiment 1 were to the continuous gastric infusion of rat 3 months, the fur of animal, the colour of skin, ingest, activity, the blood parameters such as routine blood test, Liver and kidney function of the animal all without exception such as urine, feces and animal main organs index all in normal range (NR), and normal kinetic indifference histopathologic examination shows, the important organs such as the heart, liver, spleen, lung, kidney, adrenal gland, tracheae, testis, ovary are without pathological change.Drug withdrawal checks above indices all without overt toxicity reaction after two weeks, show without cumulative toxicity.
Embodiment 5 feed
Chinese medicine composition feed addictive 60kg corn 300kg, dregs of beans 150kg, salt 4kg, fish meal 35kg, soya-bean oil 25kg, zinc methionine 1kg, Copper lysinate 1kg, tryptophan 1kg, vitamin A 2kg, vitamin E 2kg, ethoxyquinoline 3kg, embodiment 1 prepared puts into fodder mixing machine together, after continuous uniform stirs 3 hours, put into pulverizer to pulverize, obtain feed.
Embodiment 6 Viral Assay
Strain, cell line
PRRSV PX strain, Marc-145 cell are preserved .PRRSV PX strain by Guangdong Provincial Academy Of Agricultural Sciences Veterinary Institute infecting both domestic animals and human ward and are thawed in 37 DEG C before use, are diluted to 100TCID50 for subsequent use with Marc-145 cell maintenance medium.
Medicine, reagent and culture medium
Ribavirin injection is provided by Shanghai Ha Sen Pharma Inc., and content is 0.1g/ml.Culture medium is DMEM+10%FBS.
Chinese medicine composition feed addictive embodiment 1 prepared is dissolved in water, adjust pH 7.0, filters, embedding, sterilizing, and obtaining crude drug concentration is the parenteral solution of 1g/ml.
To the mensuration of Marc-145 cell maximum safe concentration
Marc-145 cell is inoculated on 96 porocyte culture plates, cultivates 24h and form fine and close individual layer, abandon supernatant.Respectively Chinese traditional medicine composition composition injection, 0.1g/ml ribavirin injection DMEM maintenance medium are first done 10 times of dilutions, then continuous 2 doubling dilutions are remake, make 12 diluted concentrations respectively, namely parenteral solution diluted concentration is: 112,56,28,14,7,3.5,1.75,0.875,0.4375,0.2188,0.1094,0.055mg/ml; Ribavirin injection diluted concentration is: 10,5,2.5,1.25,0.625,0.3125,0.156,0.078,0.039,0.0195,0.00975,0.0049mg/ml.Each dilution factor solution is added to length have on 96 porocyte culture plates of individual layer Marc-145 cell, each dilution factor repeats 4 holes, every hole 100 μ L.Separately replace liquid as normal cell controls using DMEM cell maintenance medium, put 37 DEG C, 5%CO
2continuous 3d observation of cell pathology situation after cultivating 24h in constant temperature cell culture incubator.Finally judge that medicine is to the toxicity of cell, not occur that cytopathic maximum drug concentration is for maximum safe concentration according to cytopathy.
PRRSV standard strain TCID
50mensuration
Marc-145 cell is inoculated on 96 porocyte culture plates, cultivates 24h and form fine and close cell monolayer, abandon supernatant.PRRSV standard strain is made continuous 10 doubling dilutions and become 6 concentration, be added on 96 porocyte culture plates, each dilution factor repeats 6 holes, every hole 100 μ L, and other are all set to cell controls group, put 37 DEG C, 5%CO
2cultivate in constant temperature cell culture incubator.By inverted microscope observation of cell pathology situation after 72h, the cytopathy (CPE) that record produces, till the most high dilution of virus no longer produces new pathology.According to Reed-Muench formula, calculate the half cell infection amount (TCID of PRRSV
50).
Chinese medicine composition feed addictive is tested the interaction in vitro of PRRSV
Chinese medicine composition measures PRRSV inhibitory action on a cellular level
Marc-145 cell is inoculated on 96 porocyte culture plates, cultivates 24h and form fine and close cell monolayer, abandon supernatant.First Chinese traditional medicine composition composition injection, ribavirin injection are made continuous 2 doubling dilutions from maximum safe concentration, add on 96 porocyte culture plates, every hole 100 μ l, is placed in 37 DEG C of 2h; Again will containing 100 × TCID
50virus liquid be added to above-mentioned having added in the cell hole of medicine, each dilution factor repeats 4 holes, every hole 100 μ l.All set up normal cell controls and virus control, put 37 DEG C, 5%CO
2cultivate in constant incubator.After 24h, continuous 4d observes pathology situation.
Chinese traditional medicine composition composition injection measures PRRSV killing action on a cellular level
Marc-145 cell is inoculated on 96 porocyte culture plates, cultivates 24h and form fine and close cell monolayer, abandon supernatant.Chinese traditional medicine composition composition injection, Ribavirin solution are made 2 doubling dilutions from maximum safe concentration, adds equivalent 200 × TCID
50virus liquid, mixing is placed on 37 DEG C and hatches 2h, and add in 96 porocyte culture plates, each dilution factor repeats 4 holes, every hole 100 μ l.All set up normal cell controls and virus control, put 37 DEG C, 5%CO
2cultivate in constant incubator.After 24h, continuous 4d observes pathology situation.
Chinese traditional medicine composition composition injection measures PRRSV blocking effect on a cellular level
Marc-145 cell is inoculated on 96 porocyte culture plates, cultivates 24h and form fine and close cell monolayer, abandon supernatant.First will containing 100 × TCID
50virus liquid be added in 96 porocyte culture plates, every hole 100 μ L, be placed in 37 DEG C effect 2h after, abandon supernatant.Again radix bupleuri removing toxic substances, ribavirin injection are made 2 doubling dilutions from maximum safe concentration, be added in the above-mentioned cell hole having infected virus, each dilution factor repeats 4 holes, every hole 100 μ L.All set up normal cell controls and virus control, put 37 DEG C, 5%CO
2cultivate in constant incubator.Continuous 4d observation of cell pathology situation after 24h.
Cytopathy criterion
The percentage of cell generation pathology is represented with "+" number, 25% hereinafter referred to as CPE (+), 26% ~ 50% is expressed as CPE (++), and 51% ~ 75% is expressed as CPE (+++), and 76% ~ 100% is expressed as CPE (++++).Cell controls form is all normal, and be expressed as CPE (), virus control all produces pathology, is expressed as CPE (++++).
Results and analysis
Chinese traditional medicine composition composition injection is to the measurement result of Marc-145 cell maximum safe concentration
As known from Table 4, Chinese traditional medicine composition composition injection maximum safe concentration on Marc-145 cell is 6000 μ g/mL, and ribavirin injection maximum safe concentration is 39 μ g/mL.
Table 4
| Solution | Cell hole is without the maximum safety of medicine concentration (μ g/mL) during CPE |
| Chinese traditional medicine composition composition injection | 6000 |
| Ribavirin injection | 39 |
The TCID of PRRSV
50measurement result
According to Reed-Muench formula, calculate the TCID of PRRSV at Marc-14 cell
50for 105.5/0.1mL.
Result of the test, in Table 5-table 7.
The inhibition of table 5 couple PRRSV
| Solution | Cell hole is without the solution dilution degree (μ g/mL) during CPE |
| Chinese traditional medicine composition composition injection | 20 |
| Ribavirin injection | 0.14 |
As can be seen from Table 5, Chinese medicine composition feed addictive has certain inhibitory action to PRRSV.
The killing effect of table 6 couple PRRSV
| Solution | Cell hole is without the solution dilution degree (μ g/mL) during CPE |
| Chinese traditional medicine composition composition injection | All without CPE |
| Ribavirin injection | All without CPE |
As can be seen from Table 6, Chinese medicine composition feed addictive has good bactericidal action to PRRSV.
The blocking effect of table 7 couple PRRSV
| Solution | Cell hole is without the solution dilution degree (μ g/mL) during CPE |
| Chinese traditional medicine composition composition injection | 130 |
| Ribavirin injection | All without CPE |
As shown in Table 7, Chinese medicine composition feed addictive of the present invention has blocking effect to PRRSV.
Embodiment 7 effect test
Trial drug
Feed prepared by embodiment 4.
Interferon, 10ml (8,000,000 unit), is developed by Institute of Microorganism, Academia Sinica.
Case is originated
In December, 2010, the child care pig on multiple pig farm, the situations such as cluster generation expiratory dyspnea (abdominal respiration) in various degree, spirit are depressed, drowsiness, poor appetite, severe patient cough, asthma, respiratory rate up to 80 times/more than min, the symptoms such as ear, belly and leg cyanosis.Cut open the visible stethemia of the sick pig of inspection, occur oedema, focal pneumonia; What have has the symptom such as pleural effusion, enlargement of hilar lymph nodes.Once used multiple drug therapy as sulphadiazine clinically, penicillin and streptomycin etc. are showed no effect, and swinery carried out the immunity of swine fever, pig blue-ear disease and streptococcosis.
Random sterile blood sampling 10 parts, separation of serum, with reference to soup scape unit report method (mixed infection [J] of porcine reproductive and respiratory syndrome virus and pathogenic salmonella. Chinese animal doctor's journal, 2005 (7): 337-338.) RT-PCR that carries out PRRSV detects.
The extraction of pathological material of disease total serum IgE
Get serum 0.25ml and add 1ml Trizol reagent, incubated at room 5min, add 0.2ml chloroform, fully rock centrifuge tube 15s, incubated at room 2 ~ 3min, 4 DEG C, the centrifugal 15min of 12000r/min, transfer supernatant is in another new 1.5ml centrifuge tube, add 0.5ml isopropyl alcohol, fully rock, place 10min, 4 DEG C, the centrifugal 10min precipitate nucleic acids of 12000r/min, abandon supernatant
Add 75% ethanol 1ml, jolt, washing nucleic acid, 4 DEG C, the centrifugal 5min of 8000r/min, abandon supernatant, and drying at room temperature precipitation 5min, adds the water-soluble solution sediment of 40 μ l DEPC.
Reverse transcription
Get downstream primer GP5.20.5 μ l, 10mmol/l dNTPs0.25 μ l, 5 × reverse transcription buffer2.0 μ l and total serum IgE 7.0 μ l, 65 DEG C of water-bath 10min, then add M-MLV reverse transcriptase 0.25 μ l, mend to cumulative volume 10.0 μ l, 37 DEG C of water-bath 1h with aseptic double-distilled water.
PCR
Reaction cumulative volume 25 μ l.Upstream primer GP5.10.5 μ l, downstream primer GP5.20.5 μ l, 2.5mmol/l dNTPs 2.0 μ l, 10 × Mg
2+freebuffer 2.5 μ l, 25mmol/l Mg
2+1.5 μ l, rTaq enzyme 2.5U, cDNA 1 μ l, mends to cumulative volume 25 μ l with sterilizing distilled water.Reaction cycle parameter: 94 DEG C of denaturation 5min; 94 DEG C of sex change 45s, 59 DEG C of annealing 45s, 72 DEG C extend 60s, carry out 35 circulations altogether; Then 72 DEG C extend 10min.Get PCR primer and carry out 2% agarose gel electrophoresis.
RT-PCR testing result
10 parts of blood serum sample results are positive entirely.According to clinical symptoms, cut open inspection change and results of serological detection, being diagnosed as main pathogen is porcine reproductive and respiratory syndrome virus.
GP TH
Get disease pig 210 and be divided into 3 groups, put into 3 respectively and clean up and the isolating pig house of sterilizing.Chinese medicine fodder group (70), feed prepared by the feeding embodiment of the present invention 4, interferon group (70), injection of interferon 5ml, feeding feed simultaneously, the difference of the feed in this feed and embodiment 4 is only the Chinese medicine composition feed addictive not using embodiment 1 to prepare, and medication every day 2 times, is used in conjunction 5d.Blank group (70), not administration, feeding feed, the difference of the feed in this feed and embodiment 4 is only the Chinese medicine composition feed addictive not using embodiment 1 to prepare.
Efficacy determination
Observe the spirit of pig, search for food, body temperature, breathing, skin and the situation such as ear color and death, judge curative effect by following standard.Cure: breathe steadily after medication, spirit, diet, body temperature, skin and ear color recover normal.Take a turn for the better: after medication, symptom obviously alleviates, breathing, spirit, body temperature, diet take a turn for the better.Invalid: after medication, symptom does not alleviate, even increase the weight of or death.Total effective rate=cure rate+improvement rate.
Data processing
The difference of cure rate and total effective rate is respectively organized by t method of inspection statistical comparison.
Treatment results, in table 8.
Table 8 respectively organizes result for the treatment of
| Group | Quantity | Cure | Effectively | Total effective rate (%) |
| Chinese medicine fodder group | 70 | 53 | 13 | 94.29 |
| Interferon group | 70 | 39 | 19 | 82.86 |
| Blank group | 70 | 0 | 6 | 8.57 |
As can be seen from Table 8, adopt the feed of Chinese medicine composition provided by the invention, in control porcine reproductive and respiratory syndrome, there is significant effect.
All above-mentioned this intellectual properties of primary enforcement, not setting restriction this new product of other forms of enforcement and/or new method.Those skilled in the art will utilize this important information, and foregoing is revised, to realize similar implementation status.But all modifications or transformation belong to the right of reservation based on new product of the present invention.
The above is only preferred embodiment of the present invention, and be not restriction the present invention being made to other form, any those skilled in the art may utilize the technology contents of above-mentioned announcement to be changed or be modified as the Equivalent embodiments of equivalent variations.But everyly do not depart from technical solution of the present invention content, any simple modification, equivalent variations and the remodeling done above embodiment according to technical spirit of the present invention, still belong to the protection domain of technical solution of the present invention.
Claims (6)
1. for preventing and treating a feed for porcine reproductive and respiratory syndrome, it is characterized in that: raw material comprises corn, dregs of beans, salt, fish meal, soya-bean oil, zinc methionine, Copper lysinate, tryptophan, VitAVitE, ethoxyquinoline and the Chinese medicine composition feed addictive for preventing and treating porcine reproductive and respiratory syndrome.
2. feed as claimed in claim 1, it is characterized in that: the weight portion of each raw material is respectively corn 280 weight portion ~ 320 weight portion, dregs of beans 140 weight portion ~ 160 weight portion, salt 3 weight portion ~ 5 weight portion, fish meal 30 weight portion ~ 40 weight portion, soya-bean oil 20 weight portion ~ 30 weight portion, zinc methionine 0.5 weight portion ~ 1.5 weight portion, Copper lysinate 0.5 weight portion ~ 1.5 weight portion, tryptophan 0.5 weight portion ~ 1.5 weight portion, retinol1 weight portion ~ 2 weight portion, vitamin e1 weight portion ~ 2 weight portion, ethoxyquinoline 2 weight portion ~ 4 weight portion, described Chinese medicine composition feed addictive 50 weight portion ~ 70 weight portion.
3. feed as claimed in claim 1 or 2, is characterized in that: in described Chinese medicine composition feed addictive, each bulk drug comprises honeysuckle, cape jasmine, the wind-weed, Commeline communis, golden cypress, the red sage root, the vine of multiflower knotweed, the Radix Astragali, sweet wormwood, myrrh, giant knotweed, the membrane of a chicken's gizzard, eclipta, Radix Liriopes, Sculellaria barbata, Companumoea root, Samson St Johswort, parasitic loranthus, cassia twig, windproof, Centipeda minima, Chinese yam, Morinda officinalis, hairy euphorbia, Hygrophila salicifolia, Radix picrorrhizae and Radix Glycyrrhizae.
4. the feed as described in claims 1 to 3, is characterized in that: in described Chinese medicine composition feed addictive, the weight of each bulk drug is respectively honeysuckle 55 weight portion ~ 65 weight portion, cape jasmine 30 weight portion ~ 40 weight portion, the wind-weed 25 weight portion ~ 35 weight portion, Commeline communis 20 weight portion ~ 30 weight portion, golden cypress 30 weight portion ~ 40 weight portion, the red sage root 25 weight portion ~ 35 weight portion, the vine of multiflower knotweed 30 weight portion ~ 40 weight portion, the Radix Astragali 55 weight portion ~ 65 weight portion, sweet wormwood 25 weight portion ~ 35 weight portion, myrrh 15 weight portion ~ 25 weight portion, giant knotweed 20 weight portion ~ 30 weight portion, the membrane of a chicken's gizzard 30 weight portion ~ 40 weight portion, eclipta 25 weight portion ~ 35 weight portion, Radix Liriopes 20 weight portion ~ 30 weight portion, Sculellaria barbata 25 weight portion ~ 35 weight portion, Companumoea root 20 weight portion ~ 30 weight portion, Samson St Johswort 25 weight portion ~ 35 weight portion, parasitic loranthus 30 weight portion ~ 40 weight portion, cassia twig 20 weight portion ~ 30 weight portion, windproof 25 weight portion ~ 35 weight portions, Centipeda minima 25 weight portion ~ 35 weight portion, Chinese yam 20 weight portion ~ 30 weight portion, Morinda officinalis 20 weight portion ~ 30 weight portion, hairy euphorbia 25 weight portion ~ 35 weight portion, Hygrophila salicifolia 20 weight portion ~ 30 weight portion, Radix picrorrhizae 15 weight portion ~ 25 weight portion and Radix Glycyrrhizae 20 weight portion ~ 30 weight portion.
5. the feed as described in Claims 1-4, it is characterized in that: in described Chinese medicine composition feed addictive, the weight of each bulk drug is respectively honeysuckle 60 weight portion, cape jasmine 35 weight portion, the wind-weed 30 weight portion, Commeline communis 25 weight portion, golden cypress 35 weight portion, the red sage root 30 weight portion, the vine of multiflower knotweed 35 weight portion, the Radix Astragali 60 weight portion, sweet wormwood 30 weight portion, myrrh 20 weight portion, giant knotweed 25 weight portion, the membrane of a chicken's gizzard 35 weight portion, eclipta 30 weight portion, Radix Liriopes 25 weight portion, Sculellaria barbata 30 weight portion, Companumoea root 25 weight portion, Samson St Johswort 30 weight portion, parasitic loranthus 35 weight portion, cassia twig 25 weight portion, windproof 30 weight portions, Centipeda minima 30 weight portion, Chinese yam 25 weight portion, Morinda officinalis 25 weight portion, hairy euphorbia 30 weight portion, Hygrophila salicifolia 25 weight portion, Radix picrorrhizae 20 weight portion and Radix Glycyrrhizae 25 weight portion.
6. the preparation method of feed described in claim 1 to 5, is characterized in that, comprising:
The first step, mixes by weight by each raw material components, gets 100g mixture, add the water of 500g ~ 600g, boil 3 ~ 4 hours, filter, obtain the first filtrate, filter residue adds the water of 200g ~ 300g again, again boils 1 ~ 2 hour, filter, obtain the second filtrate, by the first filtrate and the mixing of the second filtrate, concentrated removing aqueous solvent, pulverize, dry, obtain Chinese medicine composition fodder additive;
Second step, puts into fodder mixing machine together with the described Chinese medicine composition fodder additive other raw material components in feedstuff and the first step obtained, and continuous uniform stirred after 1 ~ 3 hour, put into pulverizer and pulverized, and obtains feed.
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| CN201410536149.1A CN104222529A (en) | 2014-10-11 | 2014-10-11 | Feed for preventing porcine reproductive and respiratory syndrome |
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| CN (1) | CN104222529A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105053741A (en) * | 2015-09-23 | 2015-11-18 | 青岛华仁技术孵化器有限公司 | Feed capable of preventing and treating porcine reproductive and respiratory syndrome |
| CN105475651A (en) * | 2015-12-28 | 2016-04-13 | 青岛华仁技术孵化器有限公司 | Feed capable of preventing and treating porcine reproductive and respiratory syndrome |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101391057A (en) * | 2008-10-23 | 2009-03-25 | 田明军 | Medicine for preventing and treating porcine reproductive and respiratory syndrome |
| CN101698024A (en) * | 2009-10-28 | 2010-04-28 | 北京大北农动物保健科技有限责任公司 | Traditional Chinese medicine for treating respiratory diseases and promoting growth for swine and preparation method thereof |
| CN103495180A (en) * | 2013-09-18 | 2014-01-08 | 浙江美保龙生物技术有限公司 | Freezing and drying protecting agentCryoprotectant for porcine reproductive and respiratory syndrome compound inactivated vaccine |
| CN104069478A (en) * | 2014-07-26 | 2014-10-01 | 施瑞客（天津）生物技术有限公司 | Traditional Chinese medicine composition for preventing and treating porcine reproductive and respiratory syndrome and preparation method thereof |
| CN104082524A (en) * | 2014-05-28 | 2014-10-08 | 青岛华仁技术孵化器有限公司 | Feed for promoting growth of weaned piglets and improving immune function |
-
2014
- 2014-10-11 CN CN201410536149.1A patent/CN104222529A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101391057A (en) * | 2008-10-23 | 2009-03-25 | 田明军 | Medicine for preventing and treating porcine reproductive and respiratory syndrome |
| CN101698024A (en) * | 2009-10-28 | 2010-04-28 | 北京大北农动物保健科技有限责任公司 | Traditional Chinese medicine for treating respiratory diseases and promoting growth for swine and preparation method thereof |
| CN103495180A (en) * | 2013-09-18 | 2014-01-08 | 浙江美保龙生物技术有限公司 | Freezing and drying protecting agentCryoprotectant for porcine reproductive and respiratory syndrome compound inactivated vaccine |
| CN104082524A (en) * | 2014-05-28 | 2014-10-08 | 青岛华仁技术孵化器有限公司 | Feed for promoting growth of weaned piglets and improving immune function |
| CN104069478A (en) * | 2014-07-26 | 2014-10-01 | 施瑞客（天津）生物技术有限公司 | Traditional Chinese medicine composition for preventing and treating porcine reproductive and respiratory syndrome and preparation method thereof |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105053741A (en) * | 2015-09-23 | 2015-11-18 | 青岛华仁技术孵化器有限公司 | Feed capable of preventing and treating porcine reproductive and respiratory syndrome |
| CN105475651A (en) * | 2015-12-28 | 2016-04-13 | 青岛华仁技术孵化器有限公司 | Feed capable of preventing and treating porcine reproductive and respiratory syndrome |
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Application publication date: 20141224 |