CN104212870B - Process for fermentation production of lysine hydrochloride - Google Patents
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Abstract
The invention belongs to the field of amino acid production and discloses a process for fermentation production of lysine hydrochloride. The process comprises the following steps of 1 producing a crystalline dextrose mother solution; 2 preparing a culture medium and performing fermentation; 3 performing filtration sterilization; 4 performing decoloration; 5 performing crystallization and 6 preparing a feed. A product produced by means of the process is high in yield and purity, a large amount of industrial waste is used, the enterprise cost is reduced, pollution is reduced, and the resource utilization rate is improved.
Description
Technical field
The invention belongs to technical field of amino acid production, and in particular to a kind of technique of fermenting and producing lysine hydrochloride.
Background technology
Lysine is one of essential amino acid, can promote human development, strengthen immunologic function, and is improved maincenter god
The effect of Jing function of organizations.Lysine is alkaline essential amino acids.Due to the lysine content in cereal foods it is very low, and plus
Easily it is destroyed during work and lacks, therefore referred to as the first limiting amino acids.Lysine be human body need a kind of aminoacid, one
Plant indispensable nutrient substance.It is known that protein is the Main Ingredients and Appearance for constituting human body cell, the protein in food enters
Enter through digesting first breaks down into amino acids after human body, then human body utilizes the new human body protein of these aminoacid resynthesis again,
Such as immune antibody, digestive enzyme, plasma protein, growth hormone are all the human body proteins after synthesis.In each of synthetic protein
In planting aminoacid, 1B is most important one kind, has lacked it, and other aminoacid are just restricted or are not used, section
Scholar it be called the essential amino acids of human body first.Scientist also has found that 1B is the important substance suppression for controlling growth in humans
Most important in long element (Somatotation, ss) is also most required composition, the nervus centraliss and peripheral nervous system to people
System all plays an important role.Human body itself can not synthesize 1B, it is necessary to lysine is drawn from food and is to aid in other battalion
The key substance that foster material is fully absorbed and utilized by human body, human body only supplements enough 1Bs and could improve food
The absorption and utilization of protein, reaches balanced nutritious, enhancing development.At present, producing the main method of lysine includes two
Step fermentation method, direct fermentation, enzyme process etc..But, relatively costly, the enterprise of many production aminoacid of current fermentation medium
Loss is faced, the problem that fermentation costs are that enterprise needs to solve how is reduced.
In crystal glucose production, after Crystallization Separation glucose, substantial amounts of mother solution can be retained, these mother solution dry concentration
50% or so, reducing sugar（DE values）Content is 88% or so.Because the mother solution dry concentration is low, easily ferment, storage period is not
Can exceed that two days, in addition its water content 50% or so, limits remote way and transport, and commodity value is extremely low, and becomes new pollution
It is possible and very big.Disposing mother liquor recrystallizes extraction crystal glucose, and the quality of crystal glucose can be affected again, does not utilize and meeting
Increase production cost, cause greatly waste.
At present in crystal glucose manufacturing enterprise a part by the mother solution for producing return saccharifying operation again saccharifying, concentration,
Crystallization production crystal glucose, with the increase of mother solution recycle time, the quality of crystal glucose can decline to a great extent and preserve
Easily lump in journey, therefore need periodically outer row's mother solution；Remaining enterprise's production total sugar powder, is mainly used in the food sweet taste of low and middle-grade food
In agent, solid beverage and cookiess, but market demand is little and distribution relatively dissipates.
The content of the invention
In order to solve the above problems, entreprise cost is reduced, reduce pollution, improve resource utilization, the present invention proposes a kind of
The technique of fermenting and producing lysine hydrochloride.
To achieve these goals, the present invention is achieved by the following technical solution：
A kind of technique of fermenting and producing lysine hydrochloride, it comprises the steps：1）The generation of crystal glucose mother solution,
2）Preparation culture medium and fermentation, 3）Filtration sterilization, 4）Decolourize, 5）Crystallization and 6）Prepare feedstuff；
Specifically, comprise the following steps：
1）The generation of crystal glucose mother solution:
A：By sugar liquid activated carbon decolorizing 30min, then through ion exchange, salt and pigment are removed so that light transmittance
≥98%；
B：Pearlite filtering aid, stirring is added to decolourize 30 minutes, then filter；
C：By filtrate concentration and evaporation to 70-72%, then crystallize, centrifugation crystal and mother solution, after the drying of gained crystalline solid
Sell as finished product, it is mother liquid obtained to be crystal glucose mother solution；
2）Prepare culture medium and fermentation：
Corynebacterium glutamicum ATCC14997 and brevibacterium flavum ATCC14067 is chosen as fermentation strain；By two kinds of bacterium
Liquid is 1: 1 mixing according to volume ratio, and the concentration of two kinds of bacterium solutions is 1 × 108Individual/mL, mixed bacteria liquid is accessed in seed culture medium,
Inoculum concentration 5%, cultivates to logarithmic (log) phase, and seed liquor is obtained；The seed culture medium：Crystal glucose mother solution 2t, yeast powder 140kg,
Semen Maydis pulp 420 kg, MgSO435 kg、KH2PO463kg, para-amino benzoic acid 9.1kg, ammonium sulfate 35kg, ferrous sulfate heptahydrate
70kg, 120 DEG C of sterilizing 15min, obtains final product.
Seed liquor is seeded to into fermentation tank according to 10% inoculum concentration, aerobic fermentation is carried out, is fermented 48 hours and fermentation is obtained
Liquid, persistently adds ammonium chloride as fermentation liquid nertralizer in sweat, make zymotic fluid discharge pH in 7.0-7.2；Fermentation tank culture medium：
Glucose mother liquid 2t, the kg of Semen Maydis pulp 300, ammonium chloride 200kg, bean cake 120kg, MgSO450 kg、KH2PO4 38kg, to amino
Benzoic acid 15kg, ferrous sulfate heptahydrate 10kg, 120 DEG C of sterilizing 15min, obtain final product；
3）Filtration sterilization：
Fermentation liquid Jing micro-filtrate membrane filtrations are degerming, and trapped substance and filtered solution are collected respectively；Then filtered solution is proceeded to surpass
Membrane filtration, collects concentrated solution and penetrating fluid；Above-mentioned trapped substance and concentrated solution are merged into into mixed liquor standby；Wherein, micro-filtration membrane
For inorganic ceramic film, molecular cut off is 2000MW, and microfiltration temperature is 40 DEG C；Ultrafiltration retaining molecular weight be 300MW, ultrafiltration temperature
Spend for 40 DEG C；
4）Decolourize：
Penetrating fluid is pumped into into bleacher carries out desolventing technology, and addition in bleacher accounts for the activated carbon of penetrating fluid quality 0.5%,
The temperature controlled in bleacher is 45-50 DEG C, removes activated carbon with plate-and-frame filtration after decolouring 30min and obtains liquid A；
5）Crystallization：It is 70% that liquid A is concentrated in vacuo to into solid content, is then adjusted with hydrochloric acid and connect into multiple-effect after PH7.0
Continuous crystallizing evaporator, centrifugation obtains wet brilliant and mother solution；Finally will be wet brilliant with 50 DEG C of drying of ebullated bed, obtain final product；Running
In, multiple-effect continuous evaporating crystallizer Steam pressure control is in 0.20-0.55MPa, inlet amount 30-35m/h, effect temperature 85-96
DEG C, quadruple effect temperature 49-65 DEG C, 25Be ° of discharge concentration；
6）Prepare feedstuff：
By step 3）Gained mixed liquor and step 5）Mother liquid obtained merging obtains liquid B, then adds little toward liquid B
Wheat Straw powder, bean cake, peanut shell powder and Testa Tritici, 1000-1200 turns/min stirring 4-5min, is passed through steam and is warming up to 100 DEG C,
Distillation 15 minutes；Then distillation is dried, pulverizer is crushed and obtained final product；Wherein, wheat stalk powder, bean cake, peanut shell powder and
Testa Tritici accounts for respectively 8-10%, 5-6%, 3-4% and the 1-2% of liquid B quality.
The beneficial effect that the present invention is obtained mainly includes：
Crystal glucose waste liquor is used as the present invention raw material of fermenting lysine, reduces cost of material so that waste
Recycle, whole process is produced without garbage, and being truly realized turns waste into wealth and energy-saving and emission-reduction；The present invention adopts two kinds of zymocyte
Strain reasonable compatibility, synergism is strong, has been greatly improved fermentation efficiency；In crystal glucose mother solution of the present invention it is contained it is a large amount of also
Raw sugar, a small amount of protein, biotin just can be utilized by lysine production bacterium, and the present invention had both avoided the waste section of raw material
About cost, has produced lysine and high protein feed product again, and to enterprise rich profit is brought, and has reversed aminoacid life
Produce the loss present situation of enterprise.The present invention considerably reduces the raw material such as ammonium sulfate, liquefied ammonia, sulphuric acid in lysine hydrochloride production
Consume, overall yield had both been improve, while realizing bad ammonia salt hydrochlorate cleanly production.
Specific embodiment
In order that those skilled in the art more fully understand the technical scheme in the application, below in conjunction with the application tool
Body embodiment, is more clearly and completely described to the present invention, it is clear that described embodiment is only the application one
Divide embodiment, rather than the embodiment of whole.Based on the embodiment in the application, those of ordinary skill in the art are not making
The every other embodiment obtained under the premise of creative work, should all belong to the scope of protection of the invention.
Embodiment 1
A kind of technique of fermenting and producing lysine hydrochloride, it comprises the steps：1）The generation of crystal glucose mother solution,
2）Preparation culture medium and fermentation, 3）Filtration sterilization, 4）Decolourize, 5）Crystallization and 6）Prepare feedstuff；Specifically include following steps：
1）The generation of crystal glucose mother solution:
A：By the obtained sugar liquid activated carbon decolorizing 30min of corn starch enzymolysis, then through ion exchange, by salt and color
Element is removed so that light transmittance >=98%；
B：Pearlite filtering aid, stirring is added to decolourize 30 minutes, then filter；
C：By filtrate concentration and evaporation to 70%, then crystallize, centrifugation crystal and mother solution, make after the drying of gained crystalline solid
For finished product sale, mother liquid obtained as crystal glucose mother solution；
2）Prepare culture medium and fermentation：
Choose corynebacterium glutamicum ATCC14997（Can be found in Appl Microbiol Biotechnol. 2010
Aug;87(5):1855-66. wait）With brevibacterium flavum ATCC14067（Can be found in the texts such as CN1305009A and CN1284560A
Offer）As fermentation strain；According to volume ratio it is 1: 1 mixing by two kinds of bacterium solutions, the concentration of two kinds of bacterium solutions is 1 × 108Individual/mL,
Mixed bacteria liquid is accessed in seed culture medium, and inoculum concentration 5% is cultivated to logarithmic (log) phase, and seed liquor is obtained；The seed culture medium：Crystallization
Glucose mother liquid 2t, yeast powder 140kg, Semen Maydis pulp 420 kg, MgSO435 kg、KH2PO463kg, para-amino benzoic acid 9.1kg,
Ammonium sulfate 35kg, ferrous sulfate heptahydrate 70kg, 120 DEG C of sterilizing 15min, obtain final product.
Seed liquor is seeded to into fermentation tank according to 10% inoculum concentration, aerobic fermentation is carried out, is fermented 48 hours and fermentation is obtained
Liquid, persistently adds ammonium chloride as fermentation liquid nertralizer in sweat, make fermentation liquid discharge pH in 7.0-7.2；Fermentor cultivation
Base：Glucose mother liquid 2t, the kg of Semen Maydis pulp 300, ammonium chloride 200kg, bean cake 120kg, MgSO450 kg、KH2PO4 38kg, to ammonia
Yl benzoic acid 15kg, ferrous sulfate heptahydrate 10kg, 120 DEG C of sterilizing 15min, obtain final product；Wherein, lysine content is in fermentation liquid
18.6g/100ml, compared with corynebacterium glutamicum ATCC14997 or the fermentation raising of brevibacterium flavum ATCC14067 single bacterial strains
More than 30%.
3）Filtration sterilization：
Fermentation liquid Jing micro-filtrate membrane filtrations are degerming, and trapped substance and filtered solution are collected respectively；Then filtered solution is proceeded to surpass
Membrane filtration, collects concentrated solution and penetrating fluid；Above-mentioned trapped substance and concentrated solution are merged into into mixed liquor standby；Wherein, micro-filtration membrane
For inorganic ceramic film, molecular cut off is 2000MW, and microfiltration temperature is 40 DEG C；Ultrafiltration retaining molecular weight be 300MW, ultrafiltration temperature
Spend for 40 DEG C；
4）Decolourize：
Penetrating fluid is pumped into into bleacher carries out desolventing technology, and addition in bleacher accounts for the activated carbon of penetrating fluid quality 0.5%,
The temperature controlled in bleacher is 50 DEG C, removes activated carbon with plate-and-frame filtration after decolouring 30min and obtains liquid A；
5）Crystallization：It is 70% that liquid A is concentrated in vacuo to into solid content, is then adjusted with hydrochloric acid and connect into multiple-effect after PH7.0
Continuous crystallizing evaporator, centrifugation obtains wet brilliant and mother solution；Finally will be wet brilliant with 50 DEG C of drying of ebullated bed, obtain final product；Running
In, multiple-effect continuous evaporating crystallizer Steam pressure control is in 0.35MPa, inlet amount 30m/h, 85 DEG C of a temperature of effect, quadruple effect temperature
55 DEG C, 25Be ° of discharge concentration；Gained crystalline content is more than 98.5%；
6）Prepare feedstuff：
By step 3）Gained mixed liquor and step 5）Mother liquid obtained merging obtains liquid B, then adds little toward liquid B
Wheat Straw powder, bean cake, peanut shell powder and Testa Tritici, 1000-1200 turns/min stirring 4-5min, is passed through steam and is warming up to 100 DEG C,
Distillation 15 minutes；Then distillation is dried, pulverizer is crushed and obtained final product；Wherein, wheat stalk powder, bean cake, peanut shell powder and
Testa Tritici accounts for respectively the 8%, 5%, 3% and 1% of liquid B quality.After testing, the forage protein content 36.7%, inorganic mineral content
5.4%, lysine component content 6.2%, remaining is starch, glucide and a small amount of other aminoacid.
Embodiment 2
A kind of technique of fermenting and producing lysine hydrochloride, it comprises the steps：1）The generation of crystal glucose mother solution,
2）Preparation culture medium and fermentation, 3）Filtration sterilization, 4）Decolourize, 5）Crystallization and 6）Prepare feedstuff；
Specifically include following steps：
1）The generation of crystal glucose mother solution:
A：By sugar liquid activated carbon decolorizing 30min, then through ion exchange, salt and pigment are removed so that light transmittance
≥98%；
B：Pearlite filtering aid, stirring is added to decolourize 30 minutes, then filter；
C：By filtrate concentration and evaporation to 72%, then crystallize, centrifugation crystal and mother solution, make after the drying of gained crystalline solid
For finished product sale, mother liquid obtained as crystal glucose mother solution；
2）Prepare culture medium and fermentation：
Corynebacterium glutamicum ATCC14997 and brevibacterium flavum ATCC14067 is chosen as fermentation strain；By two kinds of bacterium
Liquid is 1: 1 mixing according to volume ratio, and the concentration of two kinds of bacterium solutions is 1 × 108Individual/mL, mixed bacteria liquid is accessed in seed culture medium,
Inoculum concentration 5%, cultivates to logarithmic (log) phase, and seed liquor is obtained；The seed culture medium：Crystal glucose mother solution 2t, yeast powder 140kg,
Semen Maydis pulp 420 kg, MgSO435 kg、KH2PO463kg, para-amino benzoic acid 9.1kg, ammonium sulfate 35kg, ferrous sulfate heptahydrate
70kg, 120 DEG C of sterilizing 15min, obtains final product.
Seed liquor is seeded to into fermentation tank according to 10% inoculum concentration, aerobic fermentation is carried out, is fermented 48 hours and fermentation is obtained
Liquid, persistently adds ammonium chloride as fermentation liquid nertralizer in sweat, make fermentation liquid discharge pH 7.2；Fermentation tank culture medium：Portugal
Grape sugar mother solution 2t, the kg of Semen Maydis pulp 300, ammonium chloride 200kg, bean cake 120kg, MgSO450 kg、KH2PO4 38kg, p-aminophenyl
Formic acid 15kg, ferrous sulfate heptahydrate 10kg, 120 DEG C of sterilizing 15min, obtain final product；Wherein, lysine content is 18.7g/ in fermentation liquid
100ml, compared with corynebacterium glutamicum ATCC14997 or brevibacterium flavum ATCC14067 single bacterial strains fermentation raising more than 30%.
3）Filtration sterilization：
Fermentation liquid Jing micro-filtrate membrane filtrations are degerming, and trapped substance and filtered solution are collected respectively；Then filtered solution is proceeded to surpass
Membrane filtration, collects concentrated solution and penetrating fluid；Above-mentioned trapped substance and concentrated solution are merged into into mixed liquor standby；Wherein, micro-filtration membrane
For inorganic ceramic film, molecular cut off is 2000MW, and microfiltration temperature is 40 DEG C；Ultrafiltration retaining molecular weight be 300MW, ultrafiltration temperature
Spend for 40 DEG C；
4）Decolourize：
Penetrating fluid is pumped into into bleacher carries out desolventing technology, and addition in bleacher accounts for the activated carbon of penetrating fluid quality 0.5%,
The temperature controlled in bleacher is 45 DEG C, removes activated carbon with plate-and-frame filtration after decolouring 30min and obtains liquid A；
5）Crystallization：It is 70% that liquid A is concentrated in vacuo to into solid content, is then adjusted with hydrochloric acid and connect into multiple-effect after PH7.0
Continuous crystallizing evaporator, centrifugation obtains wet brilliant and mother solution；Finally will be wet brilliant with 50 DEG C of drying of ebullated bed, obtain final product；Running
In, multiple-effect continuous evaporating crystallizer Steam pressure control is in 0.55MPa, inlet amount 35m/h, 96 DEG C of a temperature of effect, quadruple effect temperature
60 DEG C, 25Be ° of discharge concentration；Gained crystalline content is more than 98.5%；
6）Prepare feedstuff：
By step 3）Gained mixed liquor and step 5）Mother liquid obtained merging obtains liquid B, then adds little toward liquid B
Wheat Straw powder, bean cake, peanut shell powder and Testa Tritici, 1200 turns/min stirring 5min are passed through steam and are warming up to 100 DEG C, distillation 15
Minute；Distillation is dried, pulverizer is crushed and obtained final product；Wherein, wheat stalk powder, bean cake, peanut shell powder and Testa Tritici are accounted for respectively
The 10%, 6%, 4% and 1% of liquid B quality.After testing, the forage protein content 37.3%, inorganic mineral content 5.1% relies ammonia
Acid constituents content 6.7%, remaining is starch, glucide and a small amount of other aminoacid.
Finally, in addition it is also necessary to it is noted that listed above is only several specific embodiments of the invention.Obviously, this
It is bright to be not limited to above example, there can also be many deformations.One of ordinary skill in the art can be from present disclosure
The all deformations directly derived or associate, are considered as protection scope of the present invention.
Claims (1)
1. a kind of technique of fermenting and producing lysine hydrochloride, it is characterised in that the technique comprises the steps：1）Crystallization Portugal
The generation of grape sugar mother solution, 2）Preparation culture medium and fermentation, 3）Filtration sterilization, 4）Decolourize, 5）Crystallization and 6）Prepare feedstuff；
Described 1）The generation of crystal glucose mother solution, comprises the steps：
A：By sugar liquid activated carbon decolorizing 30min, then through ion exchange, salt and pigment are removed so that light transmittance >=
98%；
B：Pearlite filtering aid, stirring is added to decolourize 30 minutes, then filter；
C：By filtrate concentration and evaporation to 70-72%, then crystallize, centrifugation crystal and mother solution, conduct after the drying of gained crystalline solid
Finished product is sold, mother liquid obtained to be crystal glucose mother solution；
Described 2）Culture medium and fermentation are prepared, is comprised the steps：
Corynebacterium glutamicum ATCC14997 and brevibacterium flavum ATCC14067 are mixed to get into mixing according to volume ratio for 1: 1
Bacterium solution, mixed bacteria liquid is accessed in seed culture medium, inoculum concentration 5%, and culture is obtained seed liquor；By seed liquor according to 10% inoculation
Amount is seeded to fermentation tank, carries out aerobic fermentation, and ferment 48 hours prepared fermentation liquids, in sweat persistently plus ammonium chloride is used as sending out
Zymotic fluid nertralizer, makes fermentation liquid discharge pH in 7.0-7.2；
The seed culture medium is prepared as follows：2 tons of crystal glucose mother solution, yeast powder 140kg, Semen Maydis pulp 420
kg、MgSO435 kg、KH2PO463kg, para-amino benzoic acid 9.1kg, ammonium sulfate 35kg, ferrous sulfate heptahydrate 70kg, 120 DEG C go out
Bacterium 15min, obtains final product；
The fermentation tank culture medium is prepared as follows：2 tons of glucose mother liquid, Semen Maydis pulp 300kg, ammonium chloride 200kg, bean
The dregs of rice 120kg, MgSO4 50 kg、KH2PO4 38kg, para-amino benzoic acid 15kg, ferrous sulfate heptahydrate 10kg, 120 DEG C of sterilizings
15min, obtains final product；
Described 3）Filtration sterilization, comprises the steps：
Fermentation liquid Jing micro-filtrate membrane filtrations are degerming, and trapped substance and filtered solution are collected respectively；Then filtered solution is proceeded into ultrafilter membrane
Filter, collect concentrated solution and penetrating fluid；Above-mentioned trapped substance and concentrated solution are merged into into mixed liquor standby；Wherein, the microfiltration
Film is inorganic ceramic film, and molecular cut off is 2000MW, and microfiltration temperature is 40 DEG C；The ultrafiltration retaining molecular weight is 300MW,
Ultrafiltrate temperature is 40 DEG C；
Described 4）Decolourize, comprise the steps：
Penetrating fluid is pumped into into bleacher carries out desolventing technology, and addition in bleacher accounts for the activated carbon of penetrating fluid quality 0.5%, control
Temperature in bleacher is 45-50 DEG C, removes activated carbon with plate-and-frame filtration after decolouring 30min and obtains liquid A；
Described 5）Crystallization, comprises the steps：
It is 70% that liquid A is concentrated in vacuo to into solid content, is then adjusted with hydrochloric acid and enter after PH7.0 multiple-effect continuous evaporative crystallization
Device, centrifugation obtains wet brilliant and mother solution；Finally will be wet brilliant with 50 DEG C of drying of ebullated bed, obtain final product lysine hydrochloride；
Described 6）Feedstuff is prepared, is comprised the steps：
By step 3）Gained mixed liquor and step 5）Mother liquid obtained merging obtains liquid B, then adds wheat straw toward liquid B
Stalk powder, bean cake, peanut shell powder and Testa Tritici, 1000-1200 turns/min stirring 4-5min, is subsequently passed steam and is warming up to 100 DEG C,
Distillation 15 minutes；Finally distillation is dried, pulverizer is crushed and obtained final product；Wherein, wheat stalk powder, bean cake, peanut shell powder and
Testa Tritici accounts for respectively 8-10%, 5-6%, 3-4% and the 1-2% of liquid B quality.
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| CN105925632B (en) * | 2016-07-15 | 2019-07-05 | 呼伦贝尔东北阜丰生物科技有限公司 | A kind of glutamic acid high acid technique |
| CN106544373A (en) * | 2016-11-21 | 2017-03-29 | 长沙道勤生物科技有限公司 | A kind of method for producing lysine and feedstuff |
| CN108315368B (en) * | 2018-04-26 | 2020-11-10 | 齐齐哈尔龙江阜丰生物科技有限公司 | Preparation process of lysine fermentation medium |
| CN108315369B (en) * | 2018-04-26 | 2020-11-10 | 齐齐哈尔龙江阜丰生物科技有限公司 | Lysine fermentation medium |
| CN108796004A (en) * | 2018-06-20 | 2018-11-13 | 齐齐哈尔龙江阜丰生物科技有限公司 | A kind of technique of preparing lysine through fermentation |
| CN108796026A (en) * | 2018-06-29 | 2018-11-13 | 齐齐哈尔龙江阜丰生物科技有限公司 | Production, extraction and the purifying process of lysine |
| CN110079565A (en) * | 2019-05-08 | 2019-08-02 | 江苏博晟康生物科技有限公司 | A method of the bean curd yellow pulp water fermenting and producing amino acid of conversion ratio can be improved |
| CN110791535A (en) * | 2019-12-02 | 2020-02-14 | 齐齐哈尔龙江阜丰生物科技有限公司 | Process for producing and extracting lysine |
| CN112301068B (en) * | 2020-10-29 | 2022-10-04 | 齐齐哈尔龙江阜丰生物科技有限公司 | Hydrolysis comprehensive utilization process of glucose mother liquor |
| CN113402124B (en) * | 2021-07-02 | 2022-09-16 | 内蒙古阜丰生物科技有限公司 | Method for reducing blood sugar and collecting salt from amino acid wastewater |
| CN114456961A (en) * | 2022-03-14 | 2022-05-10 | 齐齐哈尔龙江阜丰生物科技有限公司 | Method for producing high-protein yeast by using crystallized sugar mother liquor and application of high-protein yeast in lysine fermentation |
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| CN1880466A (en) * | 2005-06-13 | 2006-12-20 | 山东西王糖业有限公司 | Process producing lysine by glucose mother liquor |
| CN100422347C (en) * | 2006-02-07 | 2008-10-01 | 三达膜科技（厦门）有限公司 | Crystalline glucose producing process based on whole membrane method |
| EA201001626A1 (en) * | 2008-04-14 | 2011-06-30 | Басф Се | METHOD OF OBTAINING AQUEOUS GLUCOSE SOLUTION OF PLANT GRAINS TRITICEAE |
| CN101491323B (en) * | 2008-11-12 | 2012-12-26 | 山东阜丰生物科技开发有限公司 | New production technique of sodium glutamate |
| CN103992964B (en) * | 2014-03-21 | 2016-10-12 | 山东寿光巨能金玉米开发有限公司 | A kind of resistant to high pH value strain and fermentative Production lysine method |
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