CN100587075C - Production of edible cellulose by two-step method - Google Patents
Production of edible cellulose by two-step method Download PDFInfo
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- CN100587075C CN100587075C CN200810100225A CN200810100225A CN100587075C CN 100587075 C CN100587075 C CN 100587075C CN 200810100225 A CN200810100225 A CN 200810100225A CN 200810100225 A CN200810100225 A CN 200810100225A CN 100587075 C CN100587075 C CN 100587075C
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Abstract
The invention discloses a two-step fermentation method of producing edible cellulose, and more particularly the edible cellulose is produced through the two-step fermentation method. Firstly, a fluidnutrient medium is prepared by adding nitrogen source, carbon source and inorganic salt into water; after high temperature disinfection sterilization and refrigeration, the fluid nutrient medium is inoculated by microorganism strains to process ferment; secondly, zymotic fluid obtained from the first step fermentation is filtered, and then the nitrogen source, the carbon source and the inorganic salt are replenished in the zymotic fluid; based on the steps, the second step fermentation nutrient medium is prepared; and then the PH value is adjusted; cellulose gelatin is obtained by inoculatingthe microorganism strains producing fiber to process ferment after boiling sterilization and refrigeration. The method adopts artificial prepared nutrient medium to produce the edible cellulose; the method breaks the limitation caused by taking coconut water as production materials; the method solves the material problem of tract and season. The two-step fermentation for producing the edible cellulose has the advantages of low cost, security and sanitation. The produced edible cellulose can completely compare beauty with the traditional nata de coco in aspects of yield and quality.
Description
Technical field
The present invention belongs to the microbial fermentation technology field, specifically by microorganism substep fermentative production edible fibre.
Background technology
Sucus Cocois, is commonly called as " coconut palm fruit " or " Natta " through zymotechnique production oyster white or transparent edible cellulose gel by action of microorganisms.At present, the production of " coconut palm fruit " mainly is that acetobacter xylinum is inserted in the Sucus Cocois of spontaneous fermentation, utilize the Sucus Cocois unique nutrient components after the spontaneous fermentation, and acetobacter xylinum grows in Sucus Cocois and can produce cellulosic characteristic, obtain edible Mierocrystalline cellulose.As: the described production method of patent CN 1066926C, just set forth the production method of utilizing Sucus Cocois production " coconut palm fruit ".Aforesaid method needs a large amount of Sucus Cocois as raw materials for production, and China only abounds with coconut in torrid areas, the South Sea, along with the continuous expansion of " coconut palm fruit " industry size, the coconut resource is becoming tight day, utilizes Sucus Cocois to be subjected to the bigger restriction in region and season as raw material production " coconut palm fruit ".In addition, the spontaneous fermentation in storage, transportation of fresh Sucus Cocois is subjected to the influence of the various assorted bacterium of occurring in nature bigger, and the safety and sanitation of raw material are difficult to be protected.
" Hainan Teachers College's journal " 2005 the 3rd phase 71-75 pages or leaves, the production method of a kind of " Natta " is disclosed: watermelon husk juice, soya bean juice, orange juice or pineapple juice and Sucus Cocois are arranged in pairs or groups by a certain percentage as the main component of substratum (the wherein shared large percentage of Sucus Cocois), insert the bacterial strain that produces fiber then and produce " Natta ", but present method still needs a large amount of Sucus Cocois as substratum, in addition, higher and be subjected to the restriction in season with fruit juice or vegetables juice as the substratum production cost.
" biotechnology " 1999 the 3rd phase 27-30 page or leaf, disclose and utilized the substratum of artificial preparation to produce cellulosic method, its method is that directly being linked into the glue acetobacter with sucrose, yeast extract, peptone, Sodium phosphate dibasic, citric acid, ethanol, water etc. is in the substratum of main component, cultivated 6-7 days, and obtained Mierocrystalline cellulose; " Xibei College of Light Industry's journal " 2002 the 5th phase 69-71 pages or leaves disclose a kind of bacillus aceticus that utilizes and have produced cellulosic method, its production method is for adding a certain amount of apple fumet in the substratum of main component at sucrose, peptone, yeast extract paste, Sodium phosphate dibasic, ethanol, citric acid etc., make bacillus aceticus in the process of growth, obtain enough nutrition sources, thereby obtain the Mierocrystalline cellulose of certain output." foodstuffs industry science and technology " 2006 the 6th phase 121-122 pages or leaves disclose a kind of method of utilizing Aqua Folium Camelliae sinensis, white sugar for the raw material production bacteria cellulose, its method is that a certain amount of active dry yeast is added as substratum in sterilization cooling backs such as black tea, white sugar, water, insert acetobacter xylinum then, fermenting obtains the Mierocrystalline cellulose of certain output after 9-10 days.Aforesaid method all is that the microorganism that produces fiber is inserted in the substratum of preparation, obtain Mierocrystalline cellulose by the microorganism direct fermentation of producing fiber, the substratum of being prepared does not contain the Sucus Cocois composition, but the nutritive ingredient of substratum and the nutrient component difference of the Sucus Cocois after the spontaneous fermentation are bigger, therefore the production cycle is long, yield poorly, the cellulose gel of producing is of poor quality, often is difficult to satisfy industrial production requirement.
Summary of the invention
The objective of the invention is provides a kind of production of edible cellulose by two-step method at the deficiency that has " coconut palm fruit " production technology now, and it is to utilize microorganism to carry out substep fermentative production edible fibers element.
The technical scheme that the present invention proposes is such:
The first step is fermented: the needed nitrogenous source of microorganism growth, carbon source, inorganic salt are added to the water is made into liquid nutrient medium, contains nitrogenous source 0.1～10%, carbon source 2～10%, inorganic salt 0～10% by weight percentage in the liquid nutrient medium; The liquid nutrient medium that is made into, adjusting the medium pH value is 3.0～6.5, and through the high-temperature sterilization sterilization, after the cooling, the inoculum size by 0.1～5% inserts microbial strains and ferments, and leavening temperature is 20～50 degrees centigrade, ferments 1～7 day.
Described nitrogenous source is inorganic nitrogen or organonitrogen, as: inorganic nitrogen is ammonium sulfate or ammonium chloride, and organonitrogen is protein or amino acid, also can be a kind of or the two above mixing the in yeast powder, yeast extract, peptone, protein powder or the extractum carnis of rich in proteins; Described carbon source is various carbohydrate, comprises Dian Fentang, sucrose or high fructose syrup etc.; Described inorganic salt are meant the mineral compound that microorganism growth is had promoter action, comprise a kind of or the two above mixing the in sal epsom, calcium chloride, potassium primary phosphate, Sodium phosphate dibasic or the sodium-chlor; Described microbial strains is acetic bacteria, milk-acid bacteria or aspergillus niger mould.
Add straight juice or the vegetables juice that accounts for gross weight 10～65% as required in the liquid medium within.
Second step fermentation: with the filtering fermentation liquor of the first step fermentation acquisition, in fermented liquid, replenish nitrogenous source then, carbon source and inorganic salt, wherein nitrogenous source 0.1～10%, carbon source 2～10%, inorganic salt 0～10%, fermented liquid is as the main component of the second step fermention medium, fermented liquid accounts for 30～100% of the second step fermention medium gross weight, preferred 60%-80%, surplus is a water, then with acid for adjusting pH value to 2.0～5.5, preferred 4.0, boiling sterilization, the microbial strains that produces fiber is inserted in the cooling back, leavening temperature is 20～40 degrees centigrade, ferments 2～10 days, can obtain cellulose gel.
Described nitrogenous source is inorganic nitrogen or organonitrogen, as: inorganic nitrogen is ammonium sulfate or ammonium chloride, and organonitrogen is protein or amino acid, also can be a kind of or the two above mixing the in the yeast powder, yeast extract, peptone, protein powder, extractum carnis of rich in proteins; Described carbon source is various carbohydrate, comprises Dian Fentang, sucrose or high fructose syrup etc.; Described inorganic salt are meant the mineral compound that microorganism growth is had promoter action, comprise a kind of or the two above mixing the in sal epsom, calcium chloride, potassium primary phosphate, Sodium phosphate dibasic, the sodium-chlor.The microbial strains of described product fiber is an acetobacter xylinum.
The present invention utilizes production of edible cellulose by two-step method, and need not with the Sucus Cocois is that raw material just can be produced, and can break away from tradition " coconut palm fruit " fully and produce in the restriction aspect region and season, and cost is lower.Because the Artificial Control whole fermentation process is compared as raw materials for production with the Sucus Cocois that adopts spontaneous fermentation, has much cleaner, safe advantage.The edible fibre that adopts this law to produce is in output with can compare favourably with traditional " coconut palm really " fully qualitatively.
Embodiment
Below in conjunction with specific embodiment, further set forth the present invention.Should be appreciated that these embodiment only to be used to the present invention is described and be not used in restriction the scope of protection of present invention.
Embodiment 1
Protein powder 50 grams, sucrose 100 grams, sal epsom 10 grams, calcium chloride 10 grams, potassium primary phosphate 10 grams, sodium-chlor 10 grams, 1 kilogram in water with salt acid for adjusting pH value to 5.0, makes the first step fermentation substratum, with substratum 121 degrees centigrade of following heat kill bacterium 10 minutes, acetic bacteria is inserted in the cooling back, and 28 degrees celsius bottom fermentations 3 days are standby behind the filtering fermentation liquor.Add white sugar 50 grams to above-mentioned in the fermented liquid after the acetic bacteria fermentation, sal epsom 5 grams, stirring and dissolving, with salt acid for adjusting pH value to 3.0, boil sterilization in 20 minutes, take advantage of hot charging to go into tray, the acetobacter xylinum bacterial classification is inserted in the cooling back, and standing for fermentation is gathered in the crops the edible fibers gel under 30 degrees celsius after 4 days.
Embodiment 2
Yeast extract paste 10 grams, sucrose 50 grams, sal epsom 5 grams, calcium chloride 5 grams, potassium primary phosphate 5 grams, sodium-chlor 5 grams, pineapple juice 500 grams, water 500 grams, with salt acid for adjusting pH value to 4.0, make the first step fermentation substratum, with substratum 110 degrees centigrade of following heat kill bacterium 5 minutes, cooling back access milk-acid bacteria, 28 degrees celsius bottom fermentations 2 days, standby behind the filtering fermentation liquor.Add white sugar 80 grams to above-mentioned in the fermented liquid behind the lactobacillus-fermented, ammonium sulfate 5 grams, stirring and dissolving, with salt acid for adjusting pH value to 4.0, boil sterilization in 20 minutes, take advantage of hot charging to go into tray, the acetobacter xylinum bacterial classification is inserted in the cooling back, and standing for fermentation is gathered in the crops the edible fibers gel under 28 degrees celsius after 5 days.
Embodiment 3
Yeast powder 50 grams, sucrose 50 grams, sal epsom 10 grams, potassium primary phosphate 10 grams, ammonium sulfate 10 grams, 1 kilogram in water, make the first step fermentation substratum with salt acid for adjusting pH value to 5.5, with substratum at 121 degrees centigrade of following heat kill bacterium 5 minutes, cooling back access aspergillus niger, 25 degrees celsius bottom fermentations 3 days, standby behind the filtering fermentation liquor.Add white sugar 50 grams to above-mentioned in the fermented liquid behind the fermentation of Aspergillus niger, sal epsom 10 grams, stirring and dissolving, with salt acid for adjusting pH value to 4.0, boil sterilization in 15 minutes, take advantage of hot charging to go into tray, the acetobacter xylinum bacterial classification is inserted in the cooling back, and standing for fermentation is gathered in the crops the edible fibers gel under 30 degrees celsius after 5 days.
Claims (9)
1, a kind of method of two-step fermentation production edible fibers element is characterized in that: comprise two steps:
The first step is fermented: the needed nitrogenous source of microorganism growth, carbon source, inorganic salt are added to the water is made into liquid nutrient medium, contains nitrogenous source 0.1～10%, carbon source 2～10%, inorganic salt 0～10% by weight percentage in the liquid nutrient medium; The liquid nutrient medium that is made into, adjusting the medium pH value is 3.0～6.5, and through the high-temperature sterilization sterilization, after the cooling, the inoculum size by 0.1～5% inserts microbial strains and ferments, and leavening temperature is 20～50 degrees centigrade, ferments 1～7 day; Described microbial strains is acetic bacteria, milk-acid bacteria or black-koji mould;
Second step fermentation: with the filtering fermentation liquor of the first step fermentation acquisition, in fermented liquid, replenish nitrogenous source, carbon source and inorganic salt then, wherein nitrogenous source 0.1～10%, carbon source 2～10%, inorganic salt 0～10%, fermented liquid is as the main component of the second step fermention medium, fermented liquid accounts for 30～100% of the second step fermention medium gross weight, surplus is a water, then with acid for adjusting pH value to 2.0～5.5, boiling sterilization, the microbial strains that produces fiber is inserted in the cooling back, leavening temperature is 20～40 degrees centigrade, ferments 2～10 days, can obtain cellulose gel.
2, the method for two-step fermentation production edible fibers element according to claim 1, it is characterized in that: described nitrogenous source is inorganic nitrogen or organonitrogen.
3, the method for two-step fermentation production edible fibers element according to claim 2, it is characterized in that: described inorganic nitrogen is ammonium sulfate or ammonium chloride.
4, the method for two-step fermentation production edible fibers element according to claim 2, it is characterized in that: described organonitrogen is protein or amino acid.
5, the method for two-step fermentation production edible fibers element according to claim 2 is characterized in that: described organonitrogen is a kind of or the two above mixing the in yeast powder, yeast extract, peptone, protein powder or the extractum carnis.
6, the method for two-step fermentation production edible fibers element according to claim 1, it is characterized in that: described carbon source is various carbohydrate.
7, the method for two-step fermentation production edible fibers element according to claim 6, it is characterized in that: described carbohydrate is Dian Fentang, sucrose or high fructose syrup.
8, the method for two-step fermentation production edible fibers element according to claim 1 is characterized in that: described inorganic salt are a kind of or the two above mixing the in sal epsom, calcium chloride, potassium primary phosphate, Sodium phosphate dibasic or the sodium-chlor.
9, the method for two-step fermentation production edible fibers element according to claim 1, it is characterized in that: the microbial strains of described product fiber is an acetobacter xylinum.
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Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102127576B (en) * | 2010-12-04 | 2013-04-24 | 钟春燕 | Colored bacteria cellulose product and preparation method thereof |
| CN102329832A (en) * | 2011-09-28 | 2012-01-25 | 吴力克 | Culture medium of bacterial cellulose and applications thereof |
| CN106912558B (en) * | 2017-03-07 | 2020-08-25 | 海南椰国食品有限公司 | Fruit Natta rehydration method |
| CN107201020A (en) * | 2017-07-25 | 2017-09-26 | 安徽省科晟生态木装饰材料有限公司 | A kind of preparation method of antibacterial ecological composite plate |
| CN112515099A (en) * | 2019-09-18 | 2021-03-19 | 钟春燕 | Method for processing instant noodles at low temperature |
| CN113088545A (en) * | 2019-12-23 | 2021-07-09 | 湖北香园食品有限公司 | Method for producing bacterial cellulose by pre-fermentation of coconut pulp |
| CN117051056A (en) * | 2022-05-06 | 2023-11-14 | 海南大学 | Method for producing bacterial cellulose gel by utilizing coconut food processing tail water |
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Non-Patent Citations (2)
| Title |
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| 蔗糖二步发酵法提高细菌纤维素产量的研究. 邓毛程等.甘蔗糖业，第2期. 2004 |
| 蔗糖二步发酵法提高细菌纤维素产量的研究. 邓毛程等.甘蔗糖业，第2期. 2004 * |
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Assignee: Hainan Yeguo Foods Co., Ltd. Assignor: Zhong Chunyan Contract record no.: 2010990000535 Denomination of invention: Production of edible cellulose by two-step method Granted publication date: 20100203 License type: Common License Open date: 20080917 Record date: 20100722 |