CN104198742A - Standard machine CCD setting method-enzyme hole marking method - Google Patents
Standard machine CCD setting method-enzyme hole marking method Download PDFInfo
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- CN104198742A CN104198742A CN201410413235.3A CN201410413235A CN104198742A CN 104198742 A CN104198742 A CN 104198742A CN 201410413235 A CN201410413235 A CN 201410413235A CN 104198742 A CN104198742 A CN 104198742A
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Abstract
The invention discloses a standard machine CCD setting method-enzyme hole marking method. CCD parameters calculated by enzyme-labeled immunosorbent assay (ELISA) are better than those calculated by the prior art, and the time needed by the ELISA is short and is shortened to 3 hours from original 16 hours.
Description
Technical field
The present invention relates to technical field, particularly relate to a kind of Standard Machine CCD method to set up-enzyme mark hole method.
Background technology
Available technology adopting conventional method, is used CCD detector to carry out the detection of immunology, and required time is long, approximately need 16 hours, bring the working pressure of burdensome to operating personnel, do not met efficient mode of operation, therefore needed existing detection method to improve.
Summary of the invention
Object of the present invention is exactly to provide a kind of Standard Machine CCD method to set up-enzyme mark hole method for the defect of above-mentioned existence.The CCD parameter that enzyme linked immunosorbent assay calculates is slightly better than art methods, and required time is short, originally needs to shorten to now 3 hours in 16 hours and completes.
A kind of Standard Machine CCD method to set up-enzyme mark of the present invention hole law technology scheme is to comprise the following steps:
(1) preliminary work: be ready to He Meibiao hole, enzyme mark hole frame, one of pipettor, feed head is some, luminescent solution, HRP-IgG;
(2) application of sample: get 8, symmetrical enzyme mark hole; Add HRP-IgG, luminescent solution, after wait 1-5min, start to take;
(3) detector is prepared: set test template, and set the time shutter;
(4) start every group of test: eight symmetrical Kong Weiyi group tests simultaneously, take middle four holes of rear exchange and other four holes, then taken once;
(5) data derive: taken after one group, from data management the inside derived data, and carried out mark (detector sequence number, relevant position, which group); Until complete three groups of shootings of each position;
(6) data importing: the related data that imports corresponding detector from data management interface;
(7) data read: open the data of importing, select the corresponding position of taking, read result of calculation, and export to EXCEL file;
(8) data processing: taking middle 4 holes as reference point, the signal value that same sample is taken for first and second times is divided by and calculates ratio, and both average computation go out CCD value;
(9) data verification and comparison: after amendment CCD parameter, again add enzyme and luminescent solution to calculate CV at eight points of co-located, the CCD parameter comparison calculating with original method; After institute a little all calculates, justifying adds enzyme and luminescent solution checking.
In step (2), get 8, symmetrical enzyme mark hole, be distributed in the D3/E3/D4/E4 of enzyme frame, and other 4 points.
In step (2), HRP-IgG adds 10ul, adds luminescent solution 50ul.
In step (2), after wait 2min, start to take.
In step (3), time shutter 5-20s.
In step (3), time shutter 10s.
In step (8), data handling procedure CCD value is all made as 1000.
In step (9), require CV<3.
Beneficial effect of the present invention is: adopt a kind of Standard Machine CCD method to set up-enzyme mark of the present invention hole method, significantly shorten detection time.Originally needing to shorten to now 3 hours in 16 hours completes.
brief description of the drawings:
Figure 1 shows that the CCD parameter that enzyme linked immunosorbent assay of the present invention calculates schemes compared with the prior art.
embodiment:
In order to understand better the present invention, describe technical scheme of the present invention in detail with instantiation below, but the present invention is not limited thereto.
Embodiment 1
A kind of Standard Machine CCD method to set up-enzyme mark of the present invention hole method, comprises the following steps:
(1) preliminary work: be ready to He Meibiao hole, enzyme mark hole frame, one of pipettor, feed head is some, luminescent solution, HRP-IgG;
(2) application of sample: get 8, enzyme mark hole, be distributed in the D3/E3/D4/E4 of enzyme frame, and other 4 points; HRP-IgG adds 10ul, adds luminescent solution 50ul, after wait 2min, starts to take;
(3) detector is prepared: set test template, and set time shutter 10s;
(4) start every group of test: eight symmetrical Kong Weiyi group tests simultaneously, take middle four holes of rear exchange and other four holes, then taken once;
(5) data derive: taken after one group, from data management the inside derived data, and carried out mark (detector sequence number, relevant position, which group); Until complete three groups of shootings of each position;
(6) data importing: the related data that imports corresponding detector from data management interface;
(7) data read: open the data of importing, select the corresponding position of taking, read result of calculation, and export to EXCEL file;
(8) data processing: taking D3/E3/D4/E4 as reference point (CCD value is all made as 1000), the signal value that same sample is taken for first and second times is divided by and calculates ratio, and both average computation go out CCD value.
(9) data verification and comparison: after amendment CCD parameter, again add enzyme and luminescent solution to calculate CV at eight points of co-located, require CV<3, the CCD parameter comparison calculating with original method; After institute a little all calculates, justifying adds enzyme and luminescent solution checking.
Shooting record data are as shown in table 1:
Table 1
。
Result of calculation is as shown in table 2:
Table 2
。
The result is as shown in table 3:
Table 3
。
Justifying checking is as shown in table 4-1,4-2:
Table 4-1
Table 4-2
。
Can be found out by above data: a kind of Standard Machine CCD method to set up-enzyme mark of the present invention hole method, significantly shorten detection time.Originally needing to shorten to now 3 hours in 16 hours completes.
Claims (8)
1. Standard Machine CCD method to set up-enzyme mark hole method, is characterized in that, comprises the following steps:
(1) preliminary work: be ready to He Meibiao hole, enzyme mark hole frame, one of pipettor, feed head is some, luminescent solution, HRP-IgG;
(2) application of sample: get 8, symmetrical enzyme mark hole; Add HRP-IgG, luminescent solution, after wait 1-5min, start to take;
(3) detector is prepared: set test template, and set the time shutter;
(4) start every group of test: eight symmetrical Kong Weiyi group tests simultaneously, take middle four holes of rear exchange and other four holes, then taken once;
(5) data derive: taken after one group, from data management the inside derived data, and carried out mark; Until complete three groups of shootings of each position;
(6) data importing: the related data that imports corresponding detector from data management interface;
(7) data read: open the data of importing, select the corresponding position of taking, read result of calculation, and export to EXCEL file;
(8) data processing: taking middle 4 holes as reference point, the signal value that same sample is taken for first and second times is divided by and calculates ratio, and both average computation go out CCD value;
(9) data verification and comparison: after amendment CCD parameter, again add enzyme and luminescent solution to calculate CV at eight points of co-located, the CCD parameter comparison calculating with original method; After institute a little all calculates, justifying adds enzyme and luminescent solution checking.
2. a kind of Standard Machine CCD method to set up-enzyme mark according to claim 1 hole method, is characterized in that, in step (2), gets 8, symmetrical enzyme mark hole, is distributed in the D3/E3/D4/E4 of enzyme frame, and other 4 points.
3. a kind of Standard Machine CCD method to set up-enzyme mark according to claim 1 hole method, is characterized in that, in step (2), HRP-IgG adds 10ul, adds luminescent solution 50ul.
4. a kind of Standard Machine CCD method to set up-enzyme mark according to claim 1 hole method, is characterized in that, in step (2), after wait 2min, starts to take.
5. a kind of Standard Machine CCD method to set up-enzyme mark according to claim 1 hole method, is characterized in that, in step (3), and time shutter 5-20s.
6. a kind of Standard Machine CCD method to set up-enzyme mark according to claim 1 hole method, is characterized in that, in step (3), and time shutter 10s.
7. a kind of Standard Machine CCD method to set up-enzyme mark according to claim 1 hole method, is characterized in that, in step (8), data handling procedure CCD value is all made as 1000.
8. a kind of Standard Machine CCD method to set up-enzyme mark according to claim 1 hole method, is characterized in that, in step (9), requires CV<3.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410413235.3A CN104198742A (en) | 2014-08-20 | 2014-08-20 | Standard machine CCD setting method-enzyme hole marking method |
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| CN201410413235.3A CN104198742A (en) | 2014-08-20 | 2014-08-20 | Standard machine CCD setting method-enzyme hole marking method |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5265169A (en) * | 1990-10-31 | 1993-11-23 | Suzuki Motor Corporation | Method of discriminating particle aggregation pattern |
| JPH1137923A (en) * | 1997-07-18 | 1999-02-12 | Hitachi Koki Co Ltd | Method for discriminating aggregation image |
| JP2003083971A (en) * | 2001-09-14 | 2003-03-19 | Niigata Eng Co Ltd | Immunoreaction judging system |
| CN201184885Y (en) * | 2007-11-21 | 2009-01-21 | 上海理工大学 | Automatic detection instrument for enzyme label micropore board |
| CN103969188A (en) * | 2013-02-01 | 2014-08-06 | 苏州英敏基生物技术有限公司 | Enzyme-labeling measuring instrument detection system based on CCD or CMOS image sensor |
-
2014
- 2014-08-20 CN CN201410413235.3A patent/CN104198742A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5265169A (en) * | 1990-10-31 | 1993-11-23 | Suzuki Motor Corporation | Method of discriminating particle aggregation pattern |
| JPH1137923A (en) * | 1997-07-18 | 1999-02-12 | Hitachi Koki Co Ltd | Method for discriminating aggregation image |
| JP2003083971A (en) * | 2001-09-14 | 2003-03-19 | Niigata Eng Co Ltd | Immunoreaction judging system |
| CN201184885Y (en) * | 2007-11-21 | 2009-01-21 | 上海理工大学 | Automatic detection instrument for enzyme label micropore board |
| CN103969188A (en) * | 2013-02-01 | 2014-08-06 | 苏州英敏基生物技术有限公司 | Enzyme-labeling measuring instrument detection system based on CCD or CMOS image sensor |
Non-Patent Citations (2)
| Title |
|---|
| 杨敬燕等: "《微孔板-CCD 数码成像胶束增敏荧光法检测》", 《厦门大学学报(自然科学版)》 * |
| 苗丽等: "《酶标仪自校验方法探讨》", 《中国国境卫生检疫杂志》 * |
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Application publication date: 20141210 |