CN104193519A - Formula of culture medium for Tricholoma matsutake and preparation method of culture medium - Google Patents
Formula of culture medium for Tricholoma matsutake and preparation method of culture medium Download PDFInfo
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- CN104193519A CN104193519A CN201410431909.2A CN201410431909A CN104193519A CN 104193519 A CN104193519 A CN 104193519A CN 201410431909 A CN201410431909 A CN 201410431909A CN 104193519 A CN104193519 A CN 104193519A
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- pine
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- culture material
- culture medium
- glucose
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- 238000002360 preparation method Methods 0.000 title claims abstract description 5
- 241000121220 Tricholoma matsutake Species 0.000 title abstract description 11
- 239000001963 growth medium Substances 0.000 title abstract 6
- 235000008331 Pinus X rigitaeda Nutrition 0.000 claims abstract description 29
- 235000011613 Pinus brutia Nutrition 0.000 claims abstract description 29
- 241000018646 Pinus brutia Species 0.000 claims abstract description 29
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims abstract description 24
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 13
- 240000002791 Brassica napus Species 0.000 claims abstract description 12
- 235000004977 Brassica sinapistrum Nutrition 0.000 claims abstract description 12
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims abstract description 12
- 241000209140 Triticum Species 0.000 claims abstract description 12
- 235000021307 Triticum Nutrition 0.000 claims abstract description 12
- YYRMJZQKEFZXMX-UHFFFAOYSA-L calcium bis(dihydrogenphosphate) Chemical compound [Ca+2].OP(O)([O-])=O.OP(O)([O-])=O YYRMJZQKEFZXMX-UHFFFAOYSA-L 0.000 claims abstract description 12
- 229910000389 calcium phosphate Inorganic materials 0.000 claims abstract description 12
- 239000008103 glucose Substances 0.000 claims abstract description 12
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims abstract description 12
- 235000019341 magnesium sulphate Nutrition 0.000 claims abstract description 12
- 235000019691 monocalcium phosphate Nutrition 0.000 claims abstract description 12
- 239000002689 soil Substances 0.000 claims abstract description 12
- 239000010902 straw Substances 0.000 claims abstract description 12
- 238000000855 fermentation Methods 0.000 claims abstract description 7
- 230000004151 fermentation Effects 0.000 claims abstract description 7
- SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 claims abstract description 7
- 235000000405 Pinus densiflora Nutrition 0.000 claims abstract description 6
- 240000008670 Pinus densiflora Species 0.000 claims abstract description 6
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims abstract description 6
- 235000001727 glucose Nutrition 0.000 claims abstract description 6
- 239000002245 particle Substances 0.000 claims abstract description 6
- 239000002994 raw material Substances 0.000 claims abstract description 5
- 239000000463 material Substances 0.000 claims description 25
- 241000894006 Bacteria Species 0.000 claims description 22
- 229920000742 Cotton Polymers 0.000 claims description 11
- 241000514697 Lepidothamnus laxifolius Species 0.000 claims description 5
- 235000010450 Pino mugo Nutrition 0.000 claims description 5
- 239000007864 aqueous solution Substances 0.000 claims description 5
- 239000000243 solution Substances 0.000 claims description 5
- 239000002023 wood Substances 0.000 claims description 5
- 238000000034 method Methods 0.000 claims description 3
- 239000000203 mixture Substances 0.000 claims description 2
- 235000001674 Agaricus brunnescens Nutrition 0.000 abstract description 2
- 235000012343 cottonseed oil Nutrition 0.000 abstract 2
- 238000002156 mixing Methods 0.000 abstract 2
- 241001236210 Pinus pumila Species 0.000 abstract 1
- 239000003617 indole-3-acetic acid Substances 0.000 abstract 1
- 235000015097 nutrients Nutrition 0.000 abstract 1
- 238000010298 pulverizing process Methods 0.000 abstract 1
- 238000002791 soaking Methods 0.000 abstract 1
- 235000016709 nutrition Nutrition 0.000 description 5
- 230000035764 nutrition Effects 0.000 description 4
- 241000233866 Fungi Species 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 208000024172 Cardiovascular disease Diseases 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000003886 Glycoproteins Human genes 0.000 description 1
- 108090000288 Glycoproteins Proteins 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 241001088563 Trichotoma Species 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000003712 anti-aging effect Effects 0.000 description 1
- 230000001093 anti-cancer Effects 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 206010012601 diabetes mellitus Diseases 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 230000003053 immunization Effects 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000000474 nursing effect Effects 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 235000021122 unsaturated fatty acids Nutrition 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Mushroom Cultivation (AREA)
Abstract
The invention relates to the field of edible mushrooms and particularly relates to a formula of a culture medium for Tricholoma matsutake. The formula of the culture medium for Tricholoma matsutake comprises the following raw materials in parts by mass: 30-45 parts of pine sawdust, 5-10 parts of cottonseed hull, 5-10 parts of rapeseed cakes, 5-10 parts of sandy soil, 5-10 parts of pine needles, 5-10 parts of wheat straw, 0.5-1 part of glucose, 0.2-0.3 part of calcium superphosphate and 0.2-0.4 part of magnesium sulfate, wherein the pine sawdust is preferably sawdust of roots of Japanese red pine and/or pinus pumila of which the tree age is above 20 year olds. The preparation method comprises the steps of mixing the pine sawdust, the cottonseed hull, the rapeseed cakes, the pine needles and the wheat straw together, pouring into a pulverizer for pulverizing into particles of which the sizes are 60-100 meshes, then placing into a fermentation tank for soaking in water and retting, airing until the moisture content is 10%-15%, then evenly mixing with sandy soil, glucose, calcium superphosphate and magnesium sulfate and adding 10<-10> mole/liter indole acetic acid solution according to the mass ratio of the culture medium to water being 5 to 1. The formula has balanced and sufficient nutrients and the growth of the Tricholoma matsutake can be significantly accelerated by virtue of the culture medium.
Description
Technical field
The present invention relates to edible mushrooms field, be specifically related to a kind of loose bacterium culture material formula.
Background technology
Pine bacterium, formal name used at school Trichotoma matsutake, another name pine mushroom, closes bacterium, platform bacterium, is rare famous and precious natural medicinal fungus, China's secondary protection species in imminent danger in the world.There is the area such as Chan Rong district, Shangri-la, He Yan Bian Chanrong district of Chan Rong district, Chuxiong in main Chan Rong district of China, and wherein Chan Rong district in Shangri-la accounts for 70% of national ultimate production, is the matsutake outlet champion of continuous 30 years.Matsutake contains 18 seed amino acids, 14 kinds of micro elements needed by human, 49 kinds of active nutritional materials, 5 kinds of unsaturated fatty acidss, 8 kinds of VITAMIN, 2 kinds of glycoprotein, abundant food fibre and various active enzyme, separately contain 3 kinds of precious active substances, being respectively double-stranded blazei polysaccharide, the unique cancer-resisting substance of matsutake polypeptide and the whole world---matsutakealcohol, is most precious in the world natural medical fungus.Matsutake is not only balanced in nutrition, sufficient, protects the liver the multiple efficacies such as dirty but also be improved immunizing power, anticancer antitumor, treatment diabetes and cardiovascular disorder, anti-aging nursing face, short stomach.Matsutake is the natural nourishing category of globalization, and industry mainly concentrates on Europe and Japan, because there is no the comparatively culture material formula of science equilibrium, therefore the artificial large-scale cultivation of loose bacterium is restricted always.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of loose bacterium culture material formula, and this culture material formula component is easy to get, comprehensive nutrition.
Technical problem to be solved by this invention is achieved by the following technical programs:
A kind of loose bacterium culture material formula, comprises the raw material of following mass fraction:
Pine sawdust 30-45;
Cotton seed hulls 5-10;
Rapeseed cake 5-10;
Sandy soil 10-20;
Pine needle 5-10;
Wheat Straw 5-10;
Glucose 0.5-1;
Calcium superphosphate 0.2-0.3;
Magnesium sulfate 0.2-0.4;
Described pine sawdust, cotton seed hulls, rapeseed cake, pine needle, Wheat Straw is mixed and pours the particle that is ground into 80 order left and right in pulverizer into, put into again the fermentation vat retting that is soaked in water, after retting, dry to water ratio 10%-15%, mix with sandy soil, glucose, calcium superphosphate, magnesium sulfate again, then, add the 10E-10 mol/L indolylacetic acid aqueous solution by the mass ratio 5:1 of culture material and solution.
Further, preferably the more than 20 years Japanese red pine of the age of tree and/or the root of siberian dwarf pine are pulverized the root wood chip of gained to described pine sawdust.
Further, described retting process is 10-30 degrees Celsius, 20-50 days; Be preferably 25 degrees Celsius, 30 days.
Preferably, a kind of preparation method of loose bacterium culture material formula as above, comprise the raw material of following mass fraction: the more than 20 years Japanese red pine of the age of tree and/or siberian dwarf pine root wood chip 30, cotton seed hulls 8, rapeseed cake 7, sandy soil 15, pine needle 10, Wheat Straw 7, glucose 0.7, calcium superphosphate 0.3, magnesium sulfate 0.3, described pine sawdust, cotton seed hulls, rapeseed cake, pine needle, Wheat Straw is mixed to pour into and in pulverizer, is ground into 60-100 object particles, under 20-25 degrees celsius, put into be soaked in water retting 30 days of fermentation vat, after retting, dry to water ratio 10%-15%, again with sandy soil, glucose, calcium superphosphate, magnesium sulfate mixes, then, add the 10E-10 mol/L indolylacetic acid aqueous solution by the mass ratio 5:1 of culture material and solution.
The present invention has the following advantages:
1, this pine bacterium culture material formula is balanced in nutrition, comprehensively abundant, meets the growth demand of loose bacterium, can add quick-release bacteria growing speed, shortens growth cycle, expands economic benefit.
2, the raw material in this pine bacterium culture material formula is cheap and easy to get, and collocation method is simple, easy handling.
3, this culture material formula, adopts natural matter to add that the collocation of chemical supplement is used, and under the sufficient prerequisite of comprehensive nutrition, has taken into full account the self-sow environment of loose bacterium, makes this culture material loose bacteria growing preferably.
4, in this culture material layoutprocedure, natural to pine sawdust, cotton seed hulls, pine needle etc. batching is pulverized, then put into the fermentation vat retting that is soaked in water, culture material is more easily absorbed by loose bacterium.
Embodiment
Below in conjunction with embodiment, further illustrate the present invention.
A kind of loose bacterium culture material formula, the mass fraction proportioning of preferred various components is: the more than 20 years Japanese red pine of the age of tree and/or siberian dwarf pine root wood chip 30, cotton seed hulls 8, rapeseed cake 7, sandy soil 15, pine needle 10, Wheat Straw 7, glucose 0.7, calcium superphosphate 0.3, magnesium sulfate 0.3, described pine sawdust, cotton seed hulls, rapeseed cake, pine needle, Wheat Straw is mixed and pours the particle that is ground into 80 order left and right in pulverizer into, under 20-25 degrees celsius, put into be soaked in water retting 30 days of fermentation vat, after retting, dry to water ratio 10%-15%, again with sandy soil, glucose, calcium superphosphate, magnesium sulfate mixes, then, add the 10E-10 mol/L indolylacetic acid aqueous solution by the mass ratio 5:1 of culture material and solution.
The above embodiment has only expressed embodiments of the present invention; it describes comparatively concrete and detailed; but can not therefore be interpreted as the restriction to the scope of the claims of the present invention; in every case the technical scheme that adopts the form that is equal to replacement or equivalent transformation to obtain, within all should dropping on protection scope of the present invention.
Claims (4)
1. a loose bacterium culture material formula, the raw material that it is characterized in that comprising following mass fraction:
Pine sawdust 25-35;
Cotton seed hulls 5-10;
Rapeseed cake 5-10;
Sandy soil 10-20;
Pine needle 5-10;
Wheat Straw 5-10;
Glucose 0.5-1;
Calcium superphosphate 0.2-0.3;
Magnesium sulfate 0.2-0.4;
Described pine sawdust, cotton seed hulls, rapeseed cake, pine needle, Wheat Straw is mixed to pour into and in pulverizer, is ground into 60-100 object particles, put into again the fermentation vat retting that is soaked in water, after retting, dry to water ratio 10%-15%, mix with sandy soil, glucose, calcium superphosphate, magnesium sulfate again, then, add the 10E-10 mol/L indolylacetic acid aqueous solution by culture material and solution quality than 5:1.
2. the loose bacterium culture material formula of one according to claim 1, is characterized in that: described pine sawdust is the more than 20 years Japanese red pine of the age of tree and/or siberian dwarf pine root wood chip preferably.
3. the loose bacterium culture material formula of one according to claim 1, is characterized in that: described retting process is 10-30 degrees Celsius, 20-50 days; Be preferably 20-25 degrees Celsius, 30 days.
4. the preparation method of a loose bacterium culture material formula as above, it is characterized in that described loose bacterium culture material formula is preferably by mass fraction: the more than 20 years Japanese red pine of the age of tree and/or siberian dwarf pine root wood chip 30, cotton seed hulls 8, rapeseed cake 7, sandy soil 15, pine needle 10, Wheat Straw 7, glucose 0.7, calcium superphosphate 0.3, magnesium sulfate 0.3, described pine sawdust, cotton seed hulls, rapeseed cake, pine needle, Wheat Straw is mixed to pour into and in pulverizer, is ground into 60-100 object particles, under 25 degrees celsius, put into be soaked in water retting 30 days of fermentation vat, after retting, dry to water ratio 10%-15%, again with sandy soil, glucose, calcium superphosphate, magnesium sulfate mixes, then, add the 10E-10 mol/L indolylacetic acid aqueous solution by the mass ratio 5:1 of culture material and solution.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410431909.2A CN104193519B (en) | 2014-08-29 | 2014-08-29 | Formula of culture medium for Tricholoma matsutake and preparation method of culture medium |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410431909.2A CN104193519B (en) | 2014-08-29 | 2014-08-29 | Formula of culture medium for Tricholoma matsutake and preparation method of culture medium |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104193519A true CN104193519A (en) | 2014-12-10 |
| CN104193519B CN104193519B (en) | 2017-02-08 |
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| CN201410431909.2A Active CN104193519B (en) | 2014-08-29 | 2014-08-29 | Formula of culture medium for Tricholoma matsutake and preparation method of culture medium |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106866205A (en) * | 2017-01-24 | 2017-06-20 | 中国科学院昆明植物研究所 | A kind of stropharia rugoso-annulata plantation matrix and preparation method thereof |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2000224918A (en) * | 1999-02-04 | 2000-08-15 | Hyunyu Cho | Unsterilized culture solution composition and unsterilized cultivation of mushroom using the same |
| KR100420782B1 (en) * | 2001-11-23 | 2004-03-02 | 삼해상사주식회사 | The culture method of songi mushrooms hypha |
| KR100430412B1 (en) * | 2001-12-27 | 2004-05-04 | 김혁 | Media for cultivating mushroom |
| CN1910988A (en) * | 2006-06-05 | 2007-02-14 | 蒋中海 | Method for cultivation of jiangzhonghai-China |
| CN102815986A (en) * | 2011-06-08 | 2012-12-12 | 王桂芳 | Method for preparing matsutake nutrient |
| CN103467170A (en) * | 2013-08-12 | 2013-12-25 | 凤台县星展食用菌有限公司 | Tricholoma matsutake cultivation medium and preparation method thereof |
-
2014
- 2014-08-29 CN CN201410431909.2A patent/CN104193519B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2000224918A (en) * | 1999-02-04 | 2000-08-15 | Hyunyu Cho | Unsterilized culture solution composition and unsterilized cultivation of mushroom using the same |
| KR100420782B1 (en) * | 2001-11-23 | 2004-03-02 | 삼해상사주식회사 | The culture method of songi mushrooms hypha |
| KR100430412B1 (en) * | 2001-12-27 | 2004-05-04 | 김혁 | Media for cultivating mushroom |
| CN1910988A (en) * | 2006-06-05 | 2007-02-14 | 蒋中海 | Method for cultivation of jiangzhonghai-China |
| CN102815986A (en) * | 2011-06-08 | 2012-12-12 | 王桂芳 | Method for preparing matsutake nutrient |
| CN103467170A (en) * | 2013-08-12 | 2013-12-25 | 凤台县星展食用菌有限公司 | Tricholoma matsutake cultivation medium and preparation method thereof |
Non-Patent Citations (2)
| Title |
|---|
| 魏铁铮 等: "不同培养基对松口蘑菌丝生长的影响", 《食用菌学报》 * |
| 齐乐贤: "谈谈松茸及其栽培", 《辽宁林业科技》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106866205A (en) * | 2017-01-24 | 2017-06-20 | 中国科学院昆明植物研究所 | A kind of stropharia rugoso-annulata plantation matrix and preparation method thereof |
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| Publication number | Publication date |
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| CN104193519B (en) | 2017-02-08 |
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Inventor after: Ma Jianxiong Inventor after: Ma Jianhua Inventor before: Xu Weiming |
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Effective date of registration: 20160901 Address after: The three group of 221200 Jiangsu County town two village Yao Nanjing Osprey Applicant after: JIANGSU HONGSHENG EDIBLE MUSHROOMS Co.,Ltd. Address before: 516800, No. 16, South Road, Longmen County, Huizhou, Guangdong Applicant before: Xu Weiming |
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Address after: 221200 Group 3, Erbao Village, Yaoji Town, Juning County, Jiangsu Province Patentee after: Jiangsu Hongsheng Biotechnology Co.,Ltd. Address before: 221200 Group 3, Erbao Village, Yaoji Town, Juning County, Jiangsu Province Patentee before: JIANGSU HONGSHENG EDIBLE MUSHROOMS Co.,Ltd. |
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Address after: 221200, Group 3, Erbao Village, Yaoji Town, Juning County, Xuzhou City, Jiangsu Province Patentee after: Jiangsu Hongsheng Biotechnology Co.,Ltd. Country or region after: China Address before: 221200 Group 3, Erbao Village, Yaoji Town, Juning County, Jiangsu Province Patentee before: Jiangsu Hongsheng Biotechnology Co.,Ltd. Country or region before: China |