CN104173547A - Traditional Chinese medicine composition for treating prostatitis and application thereof - Google Patents

Traditional Chinese medicine composition for treating prostatitis and application thereof Download PDF

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CN104173547A
CN104173547A CN201410461218.7A CN201410461218A CN104173547A CN 104173547 A CN104173547 A CN 104173547A CN 201410461218 A CN201410461218 A CN 201410461218A CN 104173547 A CN104173547 A CN 104173547A
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chinese medicine
medicine composition
traditional chinese
semen
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王宪成
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SHANDONG WEIKANG BIOMEDICAL SCIENCE AND TECHNOLOGY CO LTD
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SHANDONG WEIKANG BIOMEDICAL SCIENCE AND TECHNOLOGY CO LTD
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Priority to CN201610149971.1A priority Critical patent/CN105535215A/en
Priority to CN201610020124.5A priority patent/CN105412321A/en
Priority to CN201610149899.2A priority patent/CN105687438A/en
Priority to CN201610150077.6A priority patent/CN105582136A/en
Priority to CN201610145713.6A priority patent/CN105582134A/en
Priority to CN201610145710.2A priority patent/CN105582133A/en
Priority to CN201610145694.7A priority patent/CN105687437A/en
Priority to CN201410461218.7A priority patent/CN104173547A/en
Priority to CN201610020122.6A priority patent/CN105412320A/en
Priority to CN201610149975.XA priority patent/CN105582135A/en
Publication of CN104173547A publication Critical patent/CN104173547A/en
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Abstract

The invention discloses a traditional Chinese medicine composition for treating prostatitis and an application thereof. The traditional Chinese medicine composition comprises a traditional Chinese medicine extract and pharmaceutic adjuvants, wherein the traditional Chinese medicine extract is prepared from the following raw materials in parts by weight: 9-15 parts of adenophora tetraphylla, 9-15 parts of semen brassicae, 17-24 parts of Chinese pholidota herb, 10-18 parts of haliotidis concha, 20-30 parts of evening primrose, 20-30 parts of folium turpiniae, 9-15 parts of conyza blinii and 6-10 parts of salix leaf, by means of water extract and alcohol precipitate. The therapeutic action on chronic abacterial prostatitis is achieved by reducing TNF-alpha and IL-8 levels in the prostate tissue of a patient, and the inflammatory reaction degree is reduced; therefore, restoration of a prostate tissue structure is promoted.

Description

One is treated prostatitic Chinese medicine composition and application thereof
Technical field
The invention belongs to Chinese medicine technical field, treat prostatitic Chinese medicine composition and application thereof in particular to one.
Background technology
Chronic nonbacterial prostatitis is a kind of common male's urological diseases, and main manifestations is pelvis area pain, is found in perineum, penis, crissum portion, urethra, pubis portion and lumbosacral region position.Paruria can show as urgent micturition, frequent micturition, dysurea and nocturia and increase etc.Due to chronic pain obstinate, patients ' life quality declines, and may have the symptom of sexual dysfunction, anxiety, depression, insomnia, hypomnesis.Multiplex antibiotic, analgesic are treated clinically at present, but antibiotic, analgesic have certain side effect, and therapeutic process patient is very painful.
Motherland's medical science has advantage to treatment of chronic prostatitis.The methods such as classical method mainly includes that oral medicinal herb, external stick, herbal enema and suppository rectally, make a general survey of current treatment thinking and method, taking blood circulation promoting and blood stasis dispelling, clearing away heat-damp and promoting diuresis is treating stranguria, the kidney invigorating etc. is that treatment rule is generally used in the treatment of clinical chronic prostatitis.And Chinese medicine prostatitis, has the advantages such as cheap and easy to get, untoward reaction is little, therefore becomes one of current study hotspot and trend.
Summary of the invention
Modern medicine is thought, in the pathogenesis of chronic nonbacterial prostatitis and prostatic special anatomical structure, rear urethral smooth muscle spasm, prostate urine instead flow, the factor analysis such as cytokine mediated immunoreation, wherein Cytokine is further remarkable.The inventor, on the basis of cytokine theory, uses the method for motherland's medical science, has found the new method of a treatment chronic nonbacterial prostatitis.Based on this Therapeutic Method, the object of the present invention is to provide a kind of Chinese medicine composition and application thereof of resistance prostatitis.
In order to realize object of the present invention, inventor studies and persistent exploration by lot of experiments, has finally obtained following technical scheme:
One is treated prostatitic Chinese medicine composition, comprise Chinese medicine extract and pharmaceutic adjuvant, described Chinese medicine extract is prepared from through decoction and alcohol sedimentation technique by the traditional Chinese medicinal material raw materials of following weight portion: Radix Adenophorae 9-15 part, Semen Sinapis Albae 9-15 part, stone Semen Benincasae 17-24 part, extra large Semen Cassiae 10-18 part, Radix Oenotherae erythrosepalae 20-30 part, leaf of Turpinia pomifera (Roxb) D O. 20-30 part, conyza blinii 9-15 part, Folium Salicis Babylonicae 6-10 part.
Preferably, the prostatitic Chinese medicine composition for the treatment of as mentioned above, described Chinese medicine extract is prepared from through decoction and alcohol sedimentation technique by the traditional Chinese medicinal material raw materials of following weight portion: Radix Adenophorae 11-13 part, Semen Sinapis Albae 11-13 part, stone Semen Benincasae 19-21 part, extra large Semen Cassiae 13-15 part, Radix Oenotherae erythrosepalae 23-26 part, leaf of Turpinia pomifera (Roxb) D O. 23-26 part, conyza blinii 11-13 part, Folium Salicis Babylonicae 7-9 part.
In a most preferred embodiment of the present invention, Chinese medicine extract as above is prepared from through decoction and alcohol sedimentation technique by the traditional Chinese medicinal material raw materials of following weight portion: 12 parts of Radix Adenophoraes, 12 parts of Semen Sinapis Albaes, 20 parts, stone Semen Benincasae, 14 parts of extra large Semen Cassiae, 25 parts of Radix Oenotherae erythrosepalaes, 25 parts of leaf of Turpinia pomifera (Roxb) D O., 12 parts of conyza bliniis, 8 parts of Folium Salicis Babylonicaves.
Preferably, the prostatitic Chinese medicine composition of above-mentioned treatment is oral formulations.Again further preferably, described oral formulations comprises oral liquid, granule, Tablet and Capsula agent.
Above-mentioned each Chinese crude drug is prepared into the preparation method of compound granular agent of the present invention, comprise the steps: to take the Radix Adenophorae 9-15 part cleaning up, Semen Sinapis Albae 9-15 part, stone Semen Benincasae 17-24 part, sea Semen Cassiae 10-18 part, Radix Oenotherae erythrosepalae 20-30 part, leaf of Turpinia pomifera (Roxb) D O. 20-30 part, conyza blinii 9-15 part, Folium Salicis Babylonicae 6-10 part, merge, decoct with water twice, collecting decoction, be evaporated to extractum, adding ethanol makes to reach 70-80% (v/v) containing alcohol amount, stir, leave standstill, filter, when filtrate decompression is condensed into 65 DEG C, relative density is 1.20-1.30, and reclaim ethanol, obtain concentrated solution, by concentrated solution lyophilization, pulverized 80 mesh sieves, obtain clear paste powder, clear paste powder is dropped in trough type mixing machine together with pharmaceutically acceptable adjuvant, stir, slowly add while stirring binding agent, to making soft material, granulate with 12 order stainless steel sifts, forced air drying under 60 DEG C of conditions, takes out, and cools and obtain granule.
It should be noted that, Chinese medicine composition of the present invention in preparation process, adopt traditional Chinese medicinal material raw materials source as follows: Radix Adenophorae is selected Campanulaceae adenophora tetraphylla adenophora tetraphylla (Thunb.) Fisch.dry root.Semen Sinapis Albae is selected crucifer Caulis et Folium Sinapis albee sinapis alba L.dry seed.Stone Semen Benincasae are selected asclepiadaceae eye tree nelumbium eye tree lotus dischidia chinensis Champ. ex Benth.dry herb.Sea Semen Cassiae is selected Trochidae animal Chlorostoma nigerrima (Gmelin) chlorostoma nigerrinma (Gmelin)shell.Radix Oenotherae erythrosepalae is selected Oenotheraceae Radix Oenotherae erythrosepalae oenothera erythrosepala Borbdry root.Leaf of Turpinia pomifera (Roxb) D O. is selected the sharp sharp Turpinia pomifera(Roxb) D O. of Staphyleaceae plant turpinia arguta(Lindl.) Seem. ［ Ochranthe arguta Lindl. ］dried leaves.Conyza blinii is selected the dry herb of the little Herba Acroptili repentis Conyza of the false fluffy platymiscium of Compositae blinii Levl..Folium Salicis Babylonicae is selected Salicaceous Plants Salix babylonica L. salix babylonica L.dried leaves.
The secretion of cytokine is the key factor that tissue is inflamed, and TNF-α and IL-8 proinflammatory gene representation play very important effect in the immunomodulating of body and the pathogenic process of chronic nonbacterial prostatitis.Inflammation can produce TNF-α under the effects such as infection, toxin, wound, these cytokines can increase chemotactic factor as the expression of IL-8, expression product is caused and damages and cause pain body tissue by mechanism separately, and regulation and control inflammatory factor indirectly inflammation-inhibiting reacts.Therefore the inventor selects TNF-α in prostata tissue, IL-8 level as the contrast index to the treatment of chronic nonbacterial prostatitis rat, found that: compared with sham operated rats, in model control group rat prostate tissue TNF-α and IL-8 level increase ( p< 0.01); Compared with model control group, adopt TNF-α and IL-8 level in the treatment group rat prostate tissue of Chinese medicine extract intervention of the present invention significantly reduce ( p< 0.01).Therefore, the present invention also provides a kind of pharmaceutical applications, that is: the application of above-mentioned Chinese medicine extract in the medicine of preparation treatment chronic nonbacterial prostatitis.
Compared with prior art, Chinese medicine composition of the present invention is brought into play the therapeutical effect to chronic nonbacterial prostatitis by TNF-α and IL-8 level in reduction patient prostata tissue, reduces inflammatory reaction degree, thereby promotes the reparation of prostata tissue structure.
Detailed description of the invention
Be below Preparation Example and the test of pesticide effectiveness example of Chinese medicine composition of the present invention, technical scheme of the present invention is done to further description, but protection scope of the present invention be not limited to these embodiment.
The preparation of embodiment 1 granule
Get the raw materials ready: Radix Adenophorae 1.2kg, Semen Sinapis Albae 1.2kg, stone Semen Benincasae 2.0kg, extra large Semen Cassiae 1.4kg, Radix Oenotherae erythrosepalae 2.5kg, leaf of Turpinia pomifera (Roxb) D O. 2.5kg, conyza blinii 1.2kg, Folium Salicis Babylonicae 0.8kg.
Preparation: take the above-mentioned Chinese crude drug cleaning up by recipe quantity, merge, pulverize, decoct with water twice, add water for the first time as 10 times of amounts of medical material weight, decoct 2h, add water for the second time as 8 times of amounts of medical material weight, decoct 1h, collecting decoction, 0.07MPa, at 65 DEG C of temperature, be evaporated to relative density and be 1.15 extractum, adding 95% (v/v) ethanol makes to reach 75% (v/v) containing alcohol amount, stir, leave standstill 24 hours, filter, filtrate is at 0.07MPa, under 65 DEG C of conditions concentrating under reduced pressure become to relative density be 1.25, and reclaim ethanol, obtain concentrated solution, by concentrated solution lyophilization, dry thing was pulverized 80 mesh sieves, obtain clear paste powder, take clear paste powder 10g, lactose 80g, microcrystalline Cellulose 80g, sodium carboxymethyl cellulose 15g, drop in trough type mixing machine, stir 20 minutes, slowly add while stirring 55% ethanol (ethanol water that volumetric concentration is 55%), to making soft material, granulate with 12 order stainless steel sifts, forced air drying under 60 DEG C of conditions, takes out, cool and obtain granule, by every bag of 10g packaging.
The preparation of embodiment 2 granules
Get the raw materials ready: Radix Adenophorae 1.5kg, Semen Sinapis Albae 1.2kg, stone Semen Benincasae 1.8kg, extra large Semen Cassiae 1.8kg, Radix Oenotherae erythrosepalae 2.0kg, leaf of Turpinia pomifera (Roxb) D O. 3.0kg, conyza blinii 1.0kg, Folium Salicis Babylonicae 1.0kg.
Preparation: take the above-mentioned traditional Chinese medicinal material raw materials cleaning up by recipe quantity, merge, pulverize, decoct with water twice, add water for the first time as 10 times of amounts of medical material weight, decoct 2h, add water for the second time as 8 times of amounts of medical material weight, decoct 1h, collecting decoction, 0.07MPa, at 65 DEG C, be evaporated to relative density and be 1.15 extractum, adding 95% (v/v) ethanol makes to reach 75% (v/v) containing alcohol amount, stir, leave standstill 24 hours, filter, filtrate is at 0.07MPa, under 65 DEG C of conditions concentrating under reduced pressure become to relative density be 1.25, and reclaim ethanol, obtain concentrated solution, by concentrated solution lyophilization, pulverized 80 mesh sieves, obtain clear paste powder, take clear paste powder 10g, lactose 80g, microcrystalline Cellulose 80g, sodium carboxymethyl cellulose 15g, drop in trough type mixing machine, stir 20 minutes, slowly add while stirring 55% ethanol (ethanol water that volumetric concentration is 55%), to making soft material, granulate with 12 order stainless steel sifts, forced air drying under 60 DEG C of conditions, takes out, cool and obtain granule, by every bag of 10g packaging.
The clinical trial research of embodiment 3 Chinese medicine granules to chronic nonbacterial prostatitis rat model
2 month female SD rat 6O, initial weight 210-250g, ad lib, drinking-water also keep 12h light and shade to replace illumination, 18～25 DEG C of indoor temperatures, humidity 45%-65%, well-ventilated, adapts to 1 week at this feeding environment before experiment.Adopt table of random number method to be only divided into the each 2O of model control group, treatment group and sham operated rats.Take after lumbar injection 10% chloral hydrate anesthesia rat, under aseptic condition in hypogastric region median incision, expose both sides seminal vesicle and prostate, rats in sham-operated group in lateral lobe of prostate gland injecting normal saline 0.2mL/ only, all the other rats are injected to respectively lateral lobe of prostate gland with 25% XIAOZHILING ZHUSHEYE 0.2mL, close abdomen.After 5 weeks, can form chronic nonbacterial prostatitis model.Pathological change is inflammatory cell infiltration in body of gland, and secretions reduces, and lumen of gland blocks, dwindles, interstitial fibrosis.Postoperative the 5th day each group start the correspondingly tested material of the following dosage of gavage: model control group is normal saline 10mL/kg; Sham operated rats is normal saline 10mL/kg; Treatment group is that clear paste powder 0.75g/kg(clear paste powder prepared by embodiment 1 is dissolved in normal saline).Respectively organize all by 1 time/d administration totally 5 weeks.
After last administration 60min, disconnected rat neck is put to death, isolate prostata tissue, shred, the ratio that adds normal saline 400 μ L in every 100mg prostata tissue mixes.Draw 20 μ L suspensions, add numeration of leukocyte liquid 0.38mL, mix, fill pond, on cell counting count board, count four jiaos of total white blood cellses in 4 large grids.ELISA test kit detects TNF-α, IL-8 expression in rat blood serum and prostata tissue homogenate.
Administration finishes the ordinary circumstance of rear observation rat, model control group rat and rats in sham-operated group comparison, movable minimizing, hypotrichosis tarnish; Treatment group rat and the comparison of model control group rat, hair is relatively glossy, and ordinary circumstance still can.In modeling process, 1 for the treatment of group unexpected death, dead 2 of model control group.
In addition, respectively organize in rat prostate tissue TNF-α and the horizontal statistical result of IL-8 in table 1.Result of the test by table 1 can find out, compared with sham operated rats, in model control group rat prostate tissue TNF-α and IL-8 level increase ( p< 0.01).Compared with model control group, treatment group rat TNF-α and IL-8 level significantly reduce ( p< 0.01).
TNF-α and the comparison of IL-8 level in the each group of table 1 rat prostate tissue
Group Sample size TNF-α（ng/L） IL-8（ng/L）
Sham operated rats 20 3.71±0.46 24.15±2.94
Model control group 18 149.28±1.02 ★★ 98.20±17.33 ★★
Treatment group 19 12.40±1.69 ▼▼ 37.09±5.18 ▼▼
Model control group and sham operated rats comparison, ★ p< 0.05; ★ ★ p< 0.01; Treatment group and model control group comparison, ▼ p< 0.05, ▼ ▼ p< 0.01.
Each group rat leukocyte counting statistics the results are shown in Table 2.Test statistics result by table 2 can find out, sham operated rats is starkly lower than other two groups; The quantity of leucocyte for the treatment of group significantly declines, with model control group relatively have significant difference ( p< 0.01).
Table 2 each group rat leukocyte and serium inorganic phosphorus lipophore are counted comparison
Group Sample size Leukocyte (10 9Individual)
Sham operated rats 20 1.80±0.21
Model control group 18 9.49±1.13 ★★
Treatment group 19 4.37±0.66 ▼▼
Model control group and sham operated rats comparison, ★ p< 0.05; ★ ★ p< 0.01;
Treatment group and model control group comparison, ▼ p< 0.05, ▼ ▼ p< 0.01.

Claims (6)

1. the prostatitic Chinese medicine composition for the treatment of, comprise Chinese medicine extract and pharmaceutic adjuvant, it is characterized in that: described Chinese medicine extract is prepared from through decoction and alcohol sedimentation technique by the traditional Chinese medicinal material raw materials of following weight portion: Radix Adenophorae 9-15 part, Semen Sinapis Albae 9-15 part, stone Semen Benincasae 17-24 part, extra large Semen Cassiae 10-18 part, Radix Oenotherae erythrosepalae 20-30 part, leaf of Turpinia pomifera (Roxb) D O. 20-30 part, conyza blinii 9-15 part, Folium Salicis Babylonicae 6-10 part.
2. the prostatitic Chinese medicine composition for the treatment of according to claim 1, is characterized in that: described Chinese medicine extract is prepared from through decoction and alcohol sedimentation technique by the traditional Chinese medicinal material raw materials of following weight portion: Radix Adenophorae 11-13 part, Semen Sinapis Albae 11-13 part, stone Semen Benincasae 19-21 part, extra large Semen Cassiae 13-15 part, Radix Oenotherae erythrosepalae 23-26 part, leaf of Turpinia pomifera (Roxb) D O. 23-26 part, conyza blinii 11-13 part, Folium Salicis Babylonicae 7-9 part.
3. treat according to claim 2 prostatitic Chinese medicine composition, it is characterized in that: described Chinese medicine extract is prepared from through decoction and alcohol sedimentation technique by the traditional Chinese medicinal material raw materials of following weight portion: 12 parts of Radix Adenophoraes, 12 parts of Semen Sinapis Albaes, 20 parts, stone Semen Benincasae, 14 parts of extra large Semen Cassiae, 25 parts of Radix Oenotherae erythrosepalaes, 25 parts of leaf of Turpinia pomifera (Roxb) D O., 12 parts of conyza bliniis, 8 parts of Folium Salicis Babylonicaves.
4. described in claim 1 or 2 or 3, treat prostatitic Chinese medicine composition, it is characterized in that: described Chinese medicine composition is oral formulations, described oral formulations comprises oral liquid, granule, Tablet and Capsula agent.
5. the application of Chinese medicine extract claimed in claim 1 in the prostatitic medicine of preparation treatment.
6. application according to claim 5, is characterized in that, described prostatitis is chronic nonbacterial prostatitis.
CN201410461218.7A 2014-09-12 2014-09-12 Traditional Chinese medicine composition for treating prostatitis and application thereof Pending CN104173547A (en)

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CN201610149899.2A CN105687438A (en) 2014-09-12 2014-09-12 Method for preparing traditional Chinese medicine composition for treating prostatitis
CN201610150077.6A CN105582136A (en) 2014-09-12 2014-09-12 Application of traditional Chinese medicine extract in preparation of medicine for treating prostatitis
CN201610145713.6A CN105582134A (en) 2014-09-12 2014-09-12 Traditional Chinese medicine composition for treating prostatitis
CN201610145710.2A CN105582133A (en) 2014-09-12 2014-09-12 Traditional Chinese medicine composition for treating prostatitis
CN201610145694.7A CN105687437A (en) 2014-09-12 2014-09-12 Traditional Chinese medicine composition for treating prostatitis
CN201410461218.7A CN104173547A (en) 2014-09-12 2014-09-12 Traditional Chinese medicine composition for treating prostatitis and application thereof
CN201610020122.6A CN105412320A (en) 2014-09-12 2014-09-12 Application of traditional Chinese medicine extract in preparation of medicine for treating chronic abacterial prostatitis
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CN201610149898.8A Division CN105663380A (en) 2014-09-12 2014-09-12 Method for preparing traditional Chinese medicine composition for treating prostatitis
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CN201610145694.7A Division CN105687437A (en) 2014-09-12 2014-09-12 Traditional Chinese medicine composition for treating prostatitis
CN201610150076.1A Division CN105663381A (en) 2014-09-12 2014-09-12 Application of Chinese herb extract to preparation of drug for treating prostatitis
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CN201610149899.2A Division CN105687438A (en) 2014-09-12 2014-09-12 Method for preparing traditional Chinese medicine composition for treating prostatitis
CN201610020122.6A Division CN105412320A (en) 2014-09-12 2014-09-12 Application of traditional Chinese medicine extract in preparation of medicine for treating chronic abacterial prostatitis
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105434982A (en) * 2015-12-22 2016-03-30 青岛市市立医院 Chronic nonbacterial prostatitis treating pharmaceutical composition

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3342407A1 (en) * 2017-01-03 2018-07-04 Dompé farmaceutici S.p.A. Il-8 inihibitors for use in the treatment of some urological disorders

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101695555A (en) * 2008-11-13 2010-04-21 赵子山 Medicament composition for treating prostatitis and prostatomegaly and preparation method thereof
CN102379965A (en) * 2011-08-14 2012-03-21 北京绿源求证科技发展有限责任公司 Traditional Chinese medicine for treating chronic prostatitis

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101695555A (en) * 2008-11-13 2010-04-21 赵子山 Medicament composition for treating prostatitis and prostatomegaly and preparation method thereof
CN102379965A (en) * 2011-08-14 2012-03-21 北京绿源求证科技发展有限责任公司 Traditional Chinese medicine for treating chronic prostatitis

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
左福贵: "辨证论治慢性***炎108例总结", 《甘肃中医》, vol. 18, no. 10, 31 December 2005 (2005-12-31), pages 25 - 26 *
黄继东、何伟珍: "中西医结合治疗慢性非淋菌性***炎168例临床分析", 《医学理论与实践》, vol. 20, no. 8, 31 December 2007 (2007-12-31), pages 928 - 929 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105434982A (en) * 2015-12-22 2016-03-30 青岛市市立医院 Chronic nonbacterial prostatitis treating pharmaceutical composition

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