CN104170730A - Rooting cultivation technology for blueberry microbody virus-free seedling - Google Patents
Rooting cultivation technology for blueberry microbody virus-free seedling Download PDFInfo
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- CN104170730A CN104170730A CN201310211239.9A CN201310211239A CN104170730A CN 104170730 A CN104170730 A CN 104170730A CN 201310211239 A CN201310211239 A CN 201310211239A CN 104170730 A CN104170730 A CN 104170730A
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Abstract
The invention discloses a rooting cultivation technology for blueberry microbody virus-free seedlings. The technology comprises the following steps: (1) seedbed arrangement; (2) virus-free seedling transplantation; (3) preparation of an ANI medicinal liquid; and (4) transplantation. The invention simplifies the rooting step in bottles, greatly increases the number of cultivation, saves time and cost for bottle rooting cultivation, and can effectively reduce the direct damage of external environment change to tender virus-free seedlings, and reach the cultivating survival rate of above 98%.
Description
Technical field
The present invention relates to field of planting, be specifically related to the blueberry microbody detoxic seedling cultivation technology of taking root.
Background technology
Along with the fast development of blueberry industry, increasing to the demand of blueberry high quality seedling.Although blueberry can be used cuttage breeding method, can obtain virus-free nursery stock by stem eye micropropagation, and reproduction coefficient is large, speed is fast, is not subject to seasonal restrictions, be applicable to industrial fast breeding.But, the seedling culture of rootage conditional request of blueberry micropropagation is higher, technical difficulty is large, generally that explant is bred and cultivated after several weeks on medium, getting the long sprouting section of 2cm is inoculated in improved culture medium, first dark processing is transferred to after 20 days again and under illumination, is cultivated 40-50 days (rooting rate reaches 30%-50%), then bottled bud seedling was taken out after adaptive processes through one week, removing of not taking root, after the bud seedling of taking root is washed to medium with clear water, transplant, transplanting survival rate reaches 50%-80%.In the bottle of the method, rooting rate is low, and the seedling that takes root of transplanting is difficult to adapt to new matrix and culture environment, easy infection, and survival rate 70%～80%, cost is relatively high.Do not increasing on the basis of seedling cost, how at the hot and humid regional rapid batch of south China, cultivating the blueberry microbody detoxification seedling of taking root, becoming the problem of our research.
Summary of the invention
The object of the invention is to overcome the shortcomings and deficiencies of traditional technology, and propose the blueberry microbody detoxic seedling cultivation technology of taking root, to solve the problem in background technology.
The blueberry microbody detoxic seedling cultivation technology of taking root, comprise the following steps: (1), seedbed arranges: in standard plastic booth, smooth good soil, wide 140cm is set on the ground, length because of canopy length different, surrounding becomes the incubator seedbed of height 10cm～15cm with the cement brick brick work that specification is 24cm (length) * 11cm (wide) * 5c (thick), bed case bottom additionally arranges microlith sand or the river sand that 5cm～8cm is thick, on fine sand face, cover the short silk water conservancy project cloth that ventilative water permeability is good, on water conservancy project cloth flat auxiliary through carbendazol soak 12 hours and the sphagna or the fountain moss 6～10cm that are filtered dry thick, and compress compacting, make culture medium,
(2), detoxic seedling is transplanted: transplant, by taking out in bottle through rejuvenation and the dark place reason cultivation bottled micropropagation aseptic detoxic seedling of 7--10 days, with clear water, medium is cleaned, neatly discharge standby;
(3), ANI liquid preparation: get respectively auxin ABT20%, NAA30%, IBA50% fully mixes, and is mixed with the ANI liquid of 1000mg/L～2000mg/L, wherein ANI liquid optium concentration is: 1300mg/L～1500mg/L;
(4), trasplanting method and step: by being ready to the aseptic bud seedling of bottled micropropagation, in the ANI liquid middling speed preparing, soak base portion 5s～10s, take out and implant on the sphagna of seedbed, implantation depth 2cm～3cm, seeding row spacing 2cm, plant good after spray painting pure water immediately, buckle the little shed moisturizing of rising 60cm, fine day will be added a cover 80% above sunshade net and be sheltered from heat or light, as canopy temperature reaches 30 degree when above, add a cover double-deck shade net, when temperature is low, remove in time sunshade net, make air humidity in little shed remain on 80--90%, temperature is 22-28 ℃, the cultivation time is removed little shed about 60 days, the rooting rate of microbody detoxic seedling reaches more than 98%.
The advantage of this method: the one, the bottled blueberry seedling of detoxification is directly shifted in the plastic tunnel of planting and carries out culture of rootage, simplified the step of taking root in bottle, greatly increased cultivation quantity, saved the cultivation cost of taking root in time and bottle; The 2nd, by the high concentration hormone drug liquid speed of taking root of allotment, soak processing, medication is few, and speed is fast, and treating capacity is large, suitable batch production batch jobs; The 3rd, after planting seedlings in southern area, to detain immediately little shed and add a cover sunshade net regulating illumination, temperature and humidity, not only economy but also material benefit, easily grasp, and can effectively alleviate external environment condition and change the direct injury to the tender detoxic seedling of children, and its cultivating survival rate is reached more than 98%.
Key problem in technology point: the one, the setting of seedling bed and the processing of matrix; The preparation of the 2nd, ANI (abbreviation of ABT, NAA, IBA) root-promoting hormone liquid, i.e. ABT20%, NAA30%, IBA50%, and solution concentration 1000mg/L～2000mg/L; The 3rd, the control of moisture and temperature and management.
Beneficial effect:
The present invention has following advantage: the one, and the bottled blueberry seedling of detoxification is directly shifted in the plastic tunnel of planting and carries out culture of rootage, simplified the step of taking root in bottle, greatly increased cultivation quantity, saved the cultivation cost of taking root in time and bottle; The 2nd, by the high concentration hormone drug liquid speed of taking root of allotment, soak processing, medication is few, and speed is fast, and treating capacity is large, suitable batch production batch jobs; The 3rd, after planting seedlings in southern area, to detain immediately little shed and add a cover sunshade net regulating illumination, temperature and humidity, not only economy but also material benefit, easily grasp, and can effectively alleviate external environment condition and change the direct injury to the tender detoxic seedling of children, and its cultivating survival rate is reached more than 98%.
Embodiment
In order to make technological means of the present invention, creation characteristic, workflow, using method reach object and effect is easy to understand, below in conjunction with tool embodiment, further set forth the present invention.
The blueberry microbody detoxic seedling cultivation technology of taking root, comprise the following steps: (1), seedbed arranges: in standard plastic booth, smooth good soil, wide 140cm is set on the ground, length because of canopy length different, surrounding becomes the incubator seedbed of height 10cm～15cm with the cement brick brick work that specification is 24cm (length) * 11cm (wide) * 5c (thick), bed case bottom additionally arranges microlith sand or the river sand that 5cm～8cm is thick, on fine sand face, cover the short silk water conservancy project cloth that ventilative water permeability is good, on water conservancy project cloth flat auxiliary through carbendazol soak 12 hours and the sphagna or the fountain moss 6～10cm that are filtered dry thick, and compress compacting, make culture medium, (2), detoxic seedling is transplanted: transplant, by taking out in bottle through rejuvenation and the dark place reason cultivation bottled micropropagation aseptic detoxic seedling of about 1 week, with clear water, medium is cleaned, neatly discharge standby, (3), ANI liquid preparation: get respectively auxin ABT20%, NAA30%, IBA50% fully mixes, and is mixed with the ANI liquid of 1500mg/L,
(4), trasplanting method and step: by being ready to the aseptic bud seedling of bottled micropropagation, in the ANI liquid middling speed preparing, soak base portion 10s, take out and implant on the sphagna of seedbed, implantation depth 3cm, seeding row spacing 2cm, plant good after spray painting pure water immediately, buckle the little shed moisturizing of rising 60cm, fine day will be added a cover 80% above sunshade net and be sheltered from heat or light, as canopy temperature reaches 30 degree when above, add a cover double-deck shade net, when temperature is low, remove in time sunshade net, make air humidity in little shed remain on 85% left and right, temperature is 22-28 ℃, the cultivation time is removed little shed about 60 days, the rooting rate of microbody detoxic seedling reaches more than 98%.
More than show and described basic principle of the present invention and principal character and advantage of the present invention.The technical staff of the industry should understand; the present invention is not restricted to the described embodiments; that in above-described embodiment and specification, describes just illustrates principle of the present invention; without departing from the spirit and scope of the present invention; the present invention also has various changes and modifications, and these changes and improvements all fall in the claimed scope of the invention.The claimed scope of the present invention is defined by appending claims and equivalent thereof.
Claims (2)
1. the blueberry microbody detoxic seedling cultivation technology of taking root, it is characterized in that, comprise the following steps: (1), seedbed arranges: in standard plastic booth, smooth good soil, wide 140cm is set on the ground, length because of canopy length different, surrounding becomes the incubator seedbed of height 10cm～15cm with the cement brick brick work that specification is 24cm (length) * 11cm (wide) * 5c (thick), bed case bottom additionally arranges microlith sand or the river sand that 5cm～8cm is thick, on fine sand face, cover the short silk water conservancy project cloth that ventilative water permeability is good, on water conservancy project cloth flat auxiliary through carbendazol soak 12 hours and the sphagna or the fountain moss 6～10cm that are filtered dry thick, and compress compacting, make culture medium,
(2), detoxic seedling is transplanted: transplant, by taking out in bottle through rejuvenation and the dark place reason cultivation bottled micropropagation aseptic detoxic seedling of 7--10 days, with clear water, medium is cleaned, neatly discharge standby;
(3), ANI liquid preparation: get respectively auxin ABT20%, NAA30%, IBA50% fully mixes, and is mixed with the ANI liquid of 1000mg/L～2000mg/L;
(4), trasplanting method and step: by being ready to the aseptic bud seedling of bottled micropropagation, in the ANI liquid middling speed preparing, soak base portion 5s～10s, take out and implant on the sphagna of seedbed, implantation depth 2cm～3cm, seeding row spacing 2cm, plant good after spray painting pure water immediately, buckle the little shed moisturizing of rising 60cm, fine day will be added a cover 80% above sunshade net and be sheltered from heat or light, as canopy temperature reaches 30 degree when above, add a cover double-deck shade net, when temperature is low, remove in time sunshade net, make air humidity in little shed remain on 80--90%, temperature is 22-28 ℃, the cultivation time is removed little shed about 60 days, the rooting rate of microbody detoxic seedling reaches more than 98%.
2. the blueberry microbody detoxic seedling according to claim 1 cultivation technology of taking root, is characterized in that, wherein ANI liquor strength is: 1300mg/L～1500mg/L.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106258434A (en) * | 2016-08-19 | 2017-01-04 | 江苏艺轩园林景观工程有限公司 | Blue berry booth Seedling inserts rear management method |
| CN109661960A (en) * | 2018-08-24 | 2019-04-23 | 伊春林业科学院 | A kind of wild bog bilberry spray micro cuttage seedling-raising technique |
| CN111631135A (en) * | 2020-07-02 | 2020-09-08 | 江苏农牧科技职业学院 | Blueberry seedling detoxification breeding method |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106258434A (en) * | 2016-08-19 | 2017-01-04 | 江苏艺轩园林景观工程有限公司 | Blue berry booth Seedling inserts rear management method |
| CN109661960A (en) * | 2018-08-24 | 2019-04-23 | 伊春林业科学院 | A kind of wild bog bilberry spray micro cuttage seedling-raising technique |
| CN109661960B (en) * | 2018-08-24 | 2021-06-15 | 伊春林业科学院 | Wild Vaccinium uliginosum twig micro-cutting seedling raising technology |
| CN111631135A (en) * | 2020-07-02 | 2020-09-08 | 江苏农牧科技职业学院 | Blueberry seedling detoxification breeding method |
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